LOCUS       NZ_KQ257863            11002 bp    DNA     linear   CON 02-JAN-2025
DEFINITION  Streptomyces stelliscabiei strain P3825 scf_652_52.1, whole genome
            shotgun sequence.
ACCESSION   NZ_KQ257863
VERSION     NZ_KQ257863.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces stelliscabiei
  ORGANISM  Streptomyces stelliscabiei
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 812938)
  AUTHORS   Harrison,J., Sapp,M., Thwaites,R. and Studholme,D.J.
  TITLE     Genome sequencing of plant-pathogenic Streptomyces species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 812938)
  AUTHORS   Harrison,J., Sapp,M., Thwaites,R. and Studholme,D.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2014) Biosciences, University of Exeter, Geoffrey
            Pope Building, Stocker Road, Exeter, Devon EX4 4QD, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Coverage                   :: 140.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001189035.1-RS_2025_01_02
            Annotation Date                   :: 01/02/2025 01:49:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,536
            CDSs (total)                      :: 9,455
            Genes (coding)                    :: 8,973
            CDSs (with protein)               :: 8,973
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 482
            CDSs (without protein)            :: 482
            Pseudo Genes (ambiguous residues) :: 0 of 482
            Pseudo Genes (frameshifted)       :: 129 of 482
            Pseudo Genes (incomplete)         :: 407 of 482
            Pseudo Genes (internal stop)      :: 20 of 482
            Pseudo Genes (multiple problems)  :: 69 of 482
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:19:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 11443
            Orig. end                         :: 22445
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KQ257863.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11002
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11002
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11002
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:6002](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6002
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    65..80
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.87"
                     /tool="antismash"
     misc_feature    complement(119..134)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.35"
                     /tool="antismash"
     gene            311..1162
                     /locus_tag="IQ64_RS20205"
                     /old_locus_tag="IQ64_21330"
     CDS             311..1162
                     /EC_number="1.1.1.25"
                     /GO_function="GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484469.1"
                     /locus_tag="IQ64_RS20205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_21330"
                     /product="shikimate dehydrogenase"
                     /protein_id="WP_233443476.1"
                     /transl_table=11
                     /translation="MAVLGSPVGRSLSPTLHRAAYAAMRLRWDYETIDCDASALPALFD
                     SLGDDWAGLCLTTPLKRAVLPLVDEVSDLALDVGGADTVVLRDGRSFGDHCGVHGIVAA
                     LRQAGLGAPGSAVVLGGGVAACAALAALREVGVREPVVVVRERGHAREAEAAAGRLGVS
                     VDLHRFEELGRLLRGVGVVVSTLPAGAADALAPTVARSGAAVLDAACSAWPTRLAKAAG
                     FVGGTVVHGLSPLLHRAERQIELLTGLTDVPVAAMRAAAHDELVRRAGLAAEPAVSGGQ
                     FV"
     gene            complement(1142..2353)
                     /locus_tag="IQ64_RS20210"
                     /old_locus_tag="IQ64_21335"
     CDS             complement(1142..2353)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484468.1"
                     /locus_tag="IQ64_RS20210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_21335"
                     /product="transposase"
                     /protein_id="WP_046919171.1"
                     /transl_table=11
                     /translation="MEGVESDDLAQTWGDVADDFVTDFCRRMLASLKRRDQRTRGEWYV
                     SGLLSVPGRKSMRALAAVTDHGAAEQSLHHFISDSSWAWAPVRQALARHLDRVLLPQAW
                     VIGSMAIPKAGAHSVGVEESFRFDLGRVVNSQQAYGVSLANEQLSVPVNWGLNLPAEWL
                     EDAERRRRARIPDDATAMSVEAGAVHTAAELADAAWGLRRRPVVLDARTLDSPALLEEI
                     TRRGIPFVMRVDGATRVLGFDPGQSGSGKQLFQARQLVERGKKLSKPVCWVDPEYSITR
                     SVLVSGTRVTMPAARRPGMPAQPDQALTLVGGWPMQKGGSPELWLSNLVDVPPAALLRL
                     GRLTRRASKDFTEVSTRVGMRDFEGRSYAGWHRHVTLCSVAHAILVLSSARRQRPGVSH
                     ELSA"
     misc_feature    complement(1751..1753)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(2446..2461)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.45"
                     /tool="antismash"
     gene            2560..3282
                     /locus_tag="IQ64_RS20215"
                     /old_locus_tag="IQ64_21340"
     CDS             2560..3282
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999108.1"
                     /locus_tag="IQ64_RS20215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_21340"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_050398603.1"
                     /transl_table=11
                     /translation="MPRQERAERTREQIVRAAAVCFDTKGFGATSLSDIVETAKVSKGA
                     LYFHFTSKEKLAQAVAARSSADWRIILRAARAPGGPVLQVLIDTTHGLAHRIRHDVVFR
                     AGLRLCDERRPGGSGHPGAYPSWRRAVALVLARAGRRGELLPGLDPRQAARMLTAATAG
                     VEMLSRQDRGWLAAATVTGVWESLLPALVPPERIGGLRPSGRPMLPAPRGPSPRGAGPR
                     ERGRPGERAGAPGRAGVG"
     gene            complement(4180..4824)
                     /locus_tag="IQ64_RS20220"
                     /old_locus_tag="IQ64_21345"
     CDS             complement(4180..4824)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326931.1"
                     /locus_tag="IQ64_RS20220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_21345"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_046918660.1"
                     /transl_table=11
                     /translation="MAQQARAVETRRLILQGAALVFDEMGYDAASIKEILSRVSVTKGA
                     LYFHFPSKEDLARGVLEEQTLHLHVSPQTSKLQEVIDLTMAVAHALPNDAMLRAGSRLA
                     LERGSIDFASDSPFLAWAKVCEELLEQARRDGEVLPHVDCHETAELIVGSFTGIQAFSQ
                     ISSALLDLEKRVSAMWKHILPSVAIPAALARLDTAPDRGARLQQAHQAAAV"
     gene            5001..6002
                     /locus_tag="IQ64_RS20225"
                     /old_locus_tag="IQ64_21350"
     CDS             5001..6002
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484462.1"
                     /locus_tag="IQ64_RS20225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_21350"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_046918659.1"
                     /sec_met_domain="AfsA (E-value: 1.2e-26, bitscore: 88.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLENDVELEAPLPLLDRPNSSDSSSKIFIPAQRGQQPRFTTTVPK
                     EYVHRAAVAEVLLTGWQRLDDTRFVASAQWPRAHSYFTSSCGEHYDPTLVAETIRQVGT
                     LLSHAEFGVPLNSHFILNGLEYTCEPEQLQIFGTPAELSLSITTANVRHRGGQLAGVDF
                     HVEIMRDGRPVGSGVAKASMLRPQVYSRIRGERLTPDARTQTPLAEPVEPALVDRRYAP
                     DVVLAPGDAPGRWLLRADQRHPILFEHPNDHIPGMVLLEAARQVTRVLVPGSTAISTDN
                     SFERYVEFDTPCLIEAEECESADPAVRVVSVRAVQDGRTAFTSTIVLAVPTA"
     misc_feature    5004..5022
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.79"
                     /tool="antismash"
     gene            complement(6201..6974)
                     /locus_tag="IQ64_RS20230"
                     /old_locus_tag="IQ64_21355"
     CDS             complement(6201..6974)
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (12-175). E-value: 1.8e-16.
                     Score: 52.5. Matches aSDomain:
                     nrpspksdomains_IQ64_RS20230_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999105.1"
                     /locus_tag="IQ64_RS20230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_21355"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_046918658.1"
                     /sec_met_domain="adh_short (E-value: 1.7e-37, bitscore:
                     124.6, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 9.1e-34, bitscore:
                     112.9, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSSGNGLLVDKVALITGASSGIGAAAARVFAREGARVLLVARRE
                     RQLADLVDALQSEGHKARYAVADVARGAEVAEAVRAAVEHFGRLDVAFNNAGYGVEQTP
                     LHLMDDETYDRIMDTNVRGVWNCLRHEITAMLETGGGSIVNTSSVGGLVATPVAAPYVT
                     SKHAVVGLTRAAAAEYAGQGIRVNAVAPGTTRSEMVNDWFRDNPGVEPMLHQLTPQSRT
                     AEPEEIAEAAAWLCSDRSSFATGVILPVDGGFTIL"
     aSDomain        complement(6450..6938)
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IQ64_RS20230_PKS_KR.1"
                     /evalue="1.80E-16"
                     /label="IQ64_RS20230_PKS_KR.1"
                     /locus_tag="IQ64_RS20230"
                     /protein_end="175"
                     /protein_start="12"
                     /score="52.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VALITGASSGIGAAAARVFAREGARVLLVARRERQLADLVDALQS
                     EGHKARYAVADVARGAEVAEAVRAAVEHFGRLDVAFNNAGYGVEQTPLHLMDDETYDRI
                     MDTNVRGVWNCLRHEITAMLETGGGSIVNTSSVGGLVATPVAAPYVTSKHAVVGLTRAA
                     "
     CDS_motif       complement(6843..6932)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IQ64_RS20230_0001"
                     /evalue="5.20E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="IQ64_RS20230"
                     /protein_end="44"
                     /protein_start="14"
                     /score="15.9"
                     /tool="antismash"
                     /translation="LITGASSGIGAAAARVFAREGARVLLVARR"
     misc_feature    7013..7027
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     gene            complement(7053..7757)
                     /locus_tag="IQ64_RS20235"
                     /old_locus_tag="IQ64_21360"
     CDS             complement(7053..7757)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484459.1"
                     /locus_tag="IQ64_RS20235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_21360"
                     /product="haloacid dehalogenase"
                     /protein_id="WP_046918657.1"
                     /transl_table=11
                     /translation="MTGGRLRHLRAAAVNIDGVMLNDTFSPVIHHFIVSRGGVYTAELE
                     RRIFSQRQAEAARVIAEAAGVAWSPEKVLEVYFRERAEYVERHGVRILDGALAMVGLLR
                     AAGLRTVCYGGLDRSHFDSALGEHADLFDDPGYVCTDAYRPGIRELVTETFGLRPDQVL
                     VIDDVARVAETARDLGVPFVGSPSDFEHGFQREAMRAVGVRHLVRSPAEIGADLLRTLD
                     AEAADGTCWQDR"
     gene            complement(7788..9818)
                     /locus_tag="IQ64_RS20240"
                     /old_locus_tag="IQ64_21365"
     CDS             complement(7788..9818)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018: isochorismate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484457.1"
                     /locus_tag="IQ64_RS20240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_21365"
                     /product="anthranilate synthase family protein"
                     /protein_id="WP_046918656.1"
                     /transl_table=11
                     /translation="MTQAVRDDPYAASPLGRILAAPHPEFALLHRPRTGPADRIEVLVG
                     DSAEVARLADIPLRSDTAGRDPGTGRPEHEVLVVIPYRQLSERGFEAVDDGAPVITLRV
                     RTQESLPVSEALAQLPDARIDLAGGGFDIDDAAYADLVRRVVEEEIGNGEGANFVVRRT
                     FVADISDYSPLLALAVFRRLLERERGAYWTFVVHVGGRTFVGATPERHLSLSDGVAVMN
                     PISGTYRYPAQGPTLTGVLSFLADAKEADELSMVVDEELKMMARVSDFGVRAKGPRLVE
                     MAGLAHTEYVVEGRATADVRDVLRETLFAPTVTGSPLESAAKVVRRFEPHGRGYYSGVL
                     ALIGRDAEGGRTLDSSILIRTAVIDAAGRMTLGVGATLVRDSDPWAEAAETRAKAAGLL
                     SALGHAPGPEPTEPPTGPGSRPASFARHPDVLAALRQRNSALSSFWLGDTGPRAHPVPA
                     LVDRRVLVVDAEDTFTAMWDHQLRALGPVVTVRRFDDAYDPDAYDLVVLGPGPGDPRRT
                     RHPKIARLRALTRRLLDEGRPFLSVCLSHQVLSGLLGLDLVRKEHPDQGVQRKIDLFGR
                     LELVGFYNTFAARANTDLIVAPGVADTVEVCRDQENGQVHAVRGPGFRSVQFHPESVLS
                     QNGLSVMADLLTSLLEERPSRPDRIRTGPRAERRDLHAPAH"
     gene            complement(9815..10492)
                     /locus_tag="IQ64_RS20245"
                     /old_locus_tag="IQ64_21370"
     CDS             complement(9815..10492)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027: isochorismatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484455.1"
                     /locus_tag="IQ64_RS20245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_21370"
                     /product="isochorismatase family protein"
                     /protein_id="WP_046918655.1"
                     /transl_table=11
                     /translation="MGIPAITAYPMPTEDELPRNTARWTVDPRRAVLLIHDMQKYFVEP
                     FPAGRSPVTELVDHVTALRERCAALGVPVAYTTQPGGMTEQQRGLLRDFWGSGMTVAQE
                     HREVVDPIRPGTGDLVLTKWRYSAFHRTRLLASMREWGRDQLIVCGVYAHVGILMTSAD
                     AFASDIQSFLVADAVADFTPEHHRFALRYAAERCAMTLTTAAVLDRLSPVPDGGHVRQE
                     AAP"
ORIGIN
        1 agcgattgag cgggccgcgc agaaaggact cttccgcgac ggccggtgcg acggcgaaca
       61 ggcggccacg atttcgtcac cttgcgggtc ggaagaagcg cggcccggcc accggaggga
      121 gaaaaagccg tgcgcgatcc gattcaccgc cggtctatgt caagtcggcg cgccacccgg
      181 gcgatccggc tcctaagctg tcccgatatc cgaaatccac cctccggcgc ggaaggagcg
      241 tcaacgatcc atggacacgg accgaaaccc cgtgggacgg acgccgcccg cggacagaag
      301 gagcaaccgc gtggcggtgc tcggatcgcc ggtgggccgc tccctgtcgc ccacgctgca
      361 tcgagccgcc tacgcggcga tgcgactgcg ctgggactac gagacgatcg actgcgacgc
      421 atccgccctg ccggcgctgt tcgactcgct cggcgacgac tgggccgggc tctgtctgac
      481 cactccgctg aagcgagccg tcctgccgct ggtcgacgag gtgtcggacc tggccctgga
      541 cgtgggcggc gcggacacgg tggtcctgcg ggacggccgg agtttcggtg accactgcgg
      601 cgtgcacggc atcgtcgccg ccctacggca ggccgggctc ggcgcgccgg gatccgccgt
      661 ggtgctgggg ggcggagtcg cggcctgcgc ggcgctggcc gccctgcggg aggtgggcgt
      721 cagggagccc gtcgtcgtgg tgcgcgagcg cggtcacgcc agggaggccg aggccgcggc
      781 cggacggctg ggcgtctcgg tcgacctgca ccgcttcgag gaactgggcc ggctgctccg
      841 gggtgtcgga gtggtggtct ccaccctgcc ggccggcgcg gccgacgccc tcgccccgac
      901 ggtcgcccgc tccggcgccg ccgtcctcga cgcggcgtgc tcggcctggc cgacccggct
      961 ggccaaggcg gcgggtttcg tcggcggcac cgtcgtccac ggactgtccc cgctgttgca
     1021 ccgggccgag cggcagatcg agctgctgac cggtctgacc gatgtgccgg tcgcggcgat
     1081 gcgcgccgcg gcccatgacg aactggtccg gcgagccggc ctggcggccg agcccgccgt
     1141 gtcaggcgga cagttcgtgt gagacaccgg ggcgctgccg gcgtgccgag gacagcacga
     1201 ggatcgcgtg cgccacggag cagagcgtca catggcggtg ccagccggcg taggagcgtc
     1261 cttcgaagtc gcgcatgccg acccgggtgc tgacctccgt gaagtccttg ctcgcccgcc
     1321 gggtcagccg tcccaggcgc agcagcgcgg caggcggcac gtccaccaga ttggacagcc
     1381 agagctcggg cgacccgccc ttctgcatcg gccatccgcc gacgagtgtc agcgcctggt
     1441 cgggctgtgc gggcatgccc ggcctgcggg cggccggcat ggtcaccctg gtaccgctca
     1501 ccaggaccga ccgggttatc gagtactccg ggtcgaccca gcagaccggc ttggacagct
     1561 tcttgccccg ctcgaccaac tgccgtgcct ggaacagctg tttgcccgaa ccggactggc
     1621 cggggtcgaa gccgagcacc cgggtcgccc cgtcgacacg catgacgaac gggattccgc
     1681 ggcgtgtgat ctcctccagc agggcggggg agtccagggt gcgggcgtcc agcacgaccg
     1741 gccgacgccg taacccccag gccgcgtcgg cgagttcggc cgccgtgtgc accgcgcccg
     1801 cctccacgct catggccgtg gcgtcgtccg ggatccgggc ccgccgtcgc cgctccgcgt
     1861 cctccagcca ctcggcgggc aggttgaggc cccagttgac gggcacgctc aactgctcgt
     1921 tggccaggga gaccccgtag gcctgctggc tgttgacgac ccggccgagg tcgaagcgga
     1981 aggactcctc gaccccgacg gagtgcgccc cggccttggg gatggccatg gagccgatca
     2041 cccatgcctg cggcagcagg acccggtcca gatggcgggc gagcgcttgc cgtacgggtg
     2101 cccacgccca ggaggagtca ctgatgaagt ggtgcagact ctgttcggcg gcgccgtggt
     2161 cggtcacggc ggccagggcg cgcatggact tgcggcccgg cacggacagc agtccggaga
     2221 cgtaccactc acccctggtg cgctggtcac gtcgcttcag tgaggccagc atccgccggc
     2281 agaagtcggt gacgaagtcg tccgcgacgt ctccccaggt ctgtgccagg tcatcgctct
     2341 ccacgccttc catggctctc cctccttctg ctgagcgtcg acagaccgac catacaatac
     2401 cggacggttg gttttctata cttcgtcatg tcgtctcgtc ccgcaagcgg tctcgacctg
     2461 aggcaataat ataccgcacg gttggtttat tgtgggagtg ttcaccggct gcactctggc
     2521 gcgggcccga ggtgagatcg ccgtgacgag ggagaggcca tgccacgcca agagcgcgcc
     2581 gaacgcacgc gcgagcagat cgtccgggcc gcggccgtgt gcttcgacac gaagggcttc
     2641 ggggcgacga gcctgagcga catcgtggag acggccaagg tctccaaagg ggccctgtac
     2701 ttccacttca cgtcgaagga gaagctggcc caggccgtgg cggcgcggtc gagcgcggac
     2761 tggcggatca tcctccgggc ggccagggca cccggcggtc cggtgctgca ggtcctgatc
     2821 gacacgacgc acggcctggc ccaccggatc aggcacgacg tcgtgttccg ggcgggcctg
     2881 cggctctgcg acgagcgccg gcccggcggc agcggccatc ccggcgcgta cccgagctgg
     2941 cggcgggccg tggcgctcgt gctcgcacgg gccggccgcc ggggcgaact actgcccggt
     3001 ctggaccccc ggcaggccgc ccgcatgctc acggcggcca ccgccggcgt cgagatgctc
     3061 tcccgccagg accgcggctg gctggccgcg gcgaccgtca ccggcgtatg ggagtccctg
     3121 ttgcccgcgc tcgtgccgcc ggagcggatc ggcggcctgc gcccctccgg gcgacccatg
     3181 ctcccggccc cgcgcgggcc gtcaccacgg ggcgccggac cgagggagcg cgggcgcccg
     3241 ggggaacggg ccggcgcgcc ggggcgggcc ggcgtgggct gagggcccgt gcgggggcgg
     3301 tgcggggcag gacggtgcgg agtgcgggcc cgcgcgccgt tgatcggcgc cgggcgcgtt
     3361 cccgatccgg cccggcccgg ccggggtggg gtccggccgc cggccgctgg tcagggcggc
     3421 cagggctcac ggggttctgg ggtgacgccg ggtgacggaa acggccctgg tgatgggggt
     3481 gtgtgccgac gcgcccgtcg ggttgccggg tcggtcggcg gtggggtggg gcggccgctg
     3541 ctcggcatcg gcggggggtt tcgcgccgct cggtggaggg ggcggggccc gtgcgccggc
     3601 tggtcgcggt gggggagggc tcccgtgctg cttcgtgggg gtggggccag gctcaggcgg
     3661 cttccgctcg ggtatcggcg gggggtgcct tgtcgtgcgt gggtgcgtgg gtgcgtgggt
     3721 gggtgcgtgg gtgggtgcgt acgggacccg tgcgctggtc gctgcgtggt gagcgccccc
     3781 acccgctcgt ccgggtcggt cgagggcggg gcggcttcgg ctcggcctgg ccgggggttc
     3841 cgtgccgcta cggccgtgcg agccggcccg tgcgcccgct cggcgcggtc aggtgagcgc
     3901 ccccgagccg cccgtgcgca tgggcgacgc ccgcccgtcg gctgctcgga ggcgcccgag
     3961 acccccgcgt cgccaccgac gtaggcaggg actcgcggcc tctgcccgaa gtgggctggg
     4021 cgctctcgcc gaacgaggtc gggcgagtgg ccgccgctgc cgctggtgct ggtccgggcg
     4081 gggccgtcct ggtggggcga gtgacctgca gccgtcgctc gcgtgggcgg gcccgggggc
     4141 ccgcgcgtcg ctggacgtcg ctgcgggccc ccggggtcac tacacggcgg ctgcctggtg
     4201 tgcctgttgc aggcgggcgc cacggtcggg ggccgtgtcg agccgggcga gggcggccgg
     4261 gatggcgacg gacggcagga tgtgcttcca catcgccgac acccgcttct ccagatccag
     4321 cagggccgag ctgatctggg agaacgcctg gatcccggtg aaggatccga cgatcagctc
     4381 cgccgtctca tggcagtcga cgtgcggcag cacctcgccg tcgcgcctgg cctgctccag
     4441 cagttcctcg cacaccttgg cccaggccag gaaggggctg tccgacgcga agtcgatgga
     4501 cccgcgctcc agcgccagac ggctcccggc gcgcagcatc gcgtcgttcg gcagtgcgtg
     4561 ggcgacggcc atggtcaggt cgatcacctc ctgcaacttg gacgtctggg gtgacacgtg
     4621 cagatggagc gtctgctcct ccagtacgcc gcgcgccagg tcctccttgg acgggaagtg
     4681 gaagtacagg gcgcccttgg tcaccgacac cctggacagg atctccttga tcgacgccgc
     4741 gtcgtagccc atctcgtcga agaccaacgc cgcgccctgc aggatgagcc gcctcgtctc
     4801 cactgcccgc gcctgctgtg ccatgcggtt tctcctgtgc tcgagtaatg ccaccgccga
     4861 ccggagcggt actcggaacg gccggtacga caacttatca agtcggtgcc ctcagacgat
     4921 ggaaaaaaac cggctgacct gtattgtgat ggagccgaca ggggttcgaa tctccttttg
     4981 ggggggacat gtccgtatca atgcttgaga acgacgtgga actggaagcc ccgcttccgc
     5041 tgctcgaccg gcccaacagc tctgattcca gctccaagat cttcatcccg gcccagagag
     5101 ggcagcagcc caggttcacc accacggtgc ccaaggagta cgtccaccgt gccgccgtgg
     5161 ccgaggtgct gctgacgggg tggcagcgcc tggacgacac ccgcttcgtg gcctccgcgc
     5221 aatggccgcg cgcccacagc tacttcacgt cctcatgcgg ggagcactac gacccgacgc
     5281 tggtagcgga gaccatccgc caggtcggca ccctgctctc gcacgccgag ttcggtgttc
     5341 cgctcaacag ccacttcatc ctgaacggcc tcgaatacac ctgtgagccc gagcagttgc
     5401 agatattcgg gacgccggcc gagctttcgc tgtccatcac caccgcgaac gtccggcaca
     5461 ggggcgggca gttggccggg gtggacttcc acgtcgagat catgcgcgac ggccggccgg
     5521 tgggctcggg cgtggcgaag gccagcatgc tccggccgca ggtctacagc cggatccgcg
     5581 gtgagcggct gacgccggac gcccggacgc agacgccgct ggccgaaccg gtcgagcccg
     5641 cgctggtcga ccgccgttac gccccggacg tggtgctcgc ccccggggac gctccgggtc
     5701 ggtggctcct gcgggccgac cagcgtcacc ccatcctctt cgagcacccc aacgaccaca
     5761 tccccgggat ggtgctgctg gaggccgccc ggcaggtcac gcgggtcctc gtccccggca
     5821 gtacggccat ctccaccgat aactccttcg agcggtacgt ggagttcgac acgccctgcc
     5881 tcatcgaggc ggaggagtgc gagtcggccg atccggcggt cagggtcgtg tccgtgcggg
     5941 ccgtacagga cggtcgtacg gcgttcacca gcaccatcgt ccttgcggtg cccaccgcgt
     6001 agaccggcgc cggggggcgg gctcgcccgg cgccacgacg acggcggcag cggcgagcat
     6061 cggacgcgag cgacgtgggc agcgcgagga gcacgacgaa cgcgatgagc acgacgagta
     6121 cttggacgcg agggcgagac gaacgtgagg agcacgacga acggggttcc gcaccacgcg
     6181 gaaccccgtt cgtcgtccgg tcagaggatc gtgaaaccgc cgtccaccgg caggatcaca
     6241 ccggtggcga acgaggagcg gtcgctgcac agccaggcgg ccgcctcggc tatctcctcg
     6301 ggctccgccg tgcgggactg cggggtgagc tgatggagca tcggctccac gcccgggttg
     6361 tcccggaacc agtcgttcac catctcgctg cgcgtcgtcc ccggagccac cgcgttgacc
     6421 cggatgccct ggccggcgta ctcggcggcc gcagcccggg tcagaccgac gaccgcgtgc
     6481 ttggacgtga cgtacggcgc cgcgaccggc gtcgccacca ggccgcccac gctgctggtg
     6541 ttgacgatgg agccgccgcc cgtctccagc atcgcggtga tctcgtggcg caggcagttc
     6601 cagacgccgc ggacgttcgt gtccatgatc cggtcgtatg tctcgtcgtc catcagatgg
     6661 agcggggtct gctccactcc gtatccggcg ttgttgaagg ccacgtccag ccgcccgaag
     6721 tgctcgacgg cagcccgtac cgcctccgcc acctcggcgc cgcgcgccac gtcggcgacc
     6781 gcgtaccggg ccttgtgtcc ctcggactgc aaggcgtcga ccaggtcggc cagttgtctc
     6841 tcccgtcggg cgacgagcag tacacgggcg ccttcgcgtg cgaagacccg ggcggcggcg
     6901 gcaccgatgc cgctgctcgc acccgtgatg agggccacct tgtcgacgag aagtccgttc
     6961 cctgaggatg tcatgcccac ttcatagcgc agtggggacg tcacggaacg gaatcacgtc
     7021 tatcaattgg tcgaagccgc aagatacttc gtttatcggt cctgccagca ggtcccgtcc
     7081 gccgcctcgg cgtccagggt gcgcagcagg tccgccccga tctcggccgg tgaccgcacc
     7141 agatgccgta cgcccaccgc gcgcatcgcc tcccgctgga agccgtgctc gaagtcgctg
     7201 gggctgccga cgaagggcac cccgaggtcg cgggcggtct cggcgaccct tgccacgtcg
     7261 tcgatgacga gcacctggtc gggccggagc ccgaacgtct cggtgacgag ttcgcggatc
     7321 ccggggcggt aggcgtcggt gcacacgtat ccgggatcgt cgaagaggtc ggcgtgctcg
     7381 ccgagggcgc tgtcgaagtg ggagcggtcg aggccgccgt agcagacggt ccgcagtccc
     7441 gcggcgcgga gcagtccgac catggcgagc gctccgtcga gaatccgcac cccgtgacgt
     7501 tcgacgtact cggcccgttc gcggaaatac acctccagga ccttctccgg ggaccaggcg
     7561 acgcccgccg cctcggcgat cacccgtgcg gcctcggcct gccgttggga gaagatccgc
     7621 cgttccagct cggccgtgta gacaccgcca cggctgacga tgaagtggtg gatcaccgga
     7681 ctgaacgtgt cgttcagcat caccccgtcg atgttcaccg cagccgcacg cagatgccgg
     7741 agccgtccgc ccgtcatgcc cttccccctt cagtcggcgg ggtcatccta gtgagcgggg
     7801 gcgtgaagat cccgtcgttc cgcgcgcggg ccggtgcgga tccggtccgg acgcgagggg
     7861 cgctcctcca ggagggaggt gagcagatcg gccatgacgc tcaggccgtt ctggctgagc
     7921 accgactccg ggtggaactg caccgagcgg aaacccggcc cgcgcacggc atgcacctgg
     7981 ccgttctcct ggtcccggca cacctccacg gtgtccgcca ccccgggggc gacgatcaga
     8041 tccgtgttcg cgcgggcggc gaaggtgttg tagaagccga ccagttcgag ccggccgaac
     8101 agatcgatct ttctctgcac cccctggtcg gggtgctcct tgcggaccag gtcgaggccg
     8161 aggagtccgc tcagtacctg gtggctcaga cacaccgaca gaaacggccg tccctcgtcc
     8221 agcagccggc gcgtgagcgc gcgcagacgg gcgatcttcg ggtgccgcgt ccgcctcggg
     8281 tccccgggcc ccggcccgag cacgacgagg tcgtaggcgt cggggtcgta ggcgtcgtcg
     8341 aagcggcgca cggtcacgac cggtcccagc gcgcgcagtt ggtggtccca catcgcggtg
     8401 aaggtgtcct ccgcgtccac caccagcacc ctccggtcga cgagcgcggg caccgggtgg
     8461 gccctcggcc cggtgtcccc cagccagaag gaggagaggg cgctgttgcg ctgccgcagg
     8521 gcggcgagga catcgggatg gcgggcgaac gacgccggcc gcgaacccgg accggtgggc
     8581 ggttcggtcg gctcgggccc gggcgcgtga ccgagggcgg acagcagccc cgccgccttc
     8641 gccctggtct cggccgcctc ggcccacgga tcggagtcgc ggaccagggt ggcgcccacg
     8701 ccgagagtca tccggccggc cgcgtcgatg acggcggttc ggatcaggat ggaggagtcc
     8761 agcgtacggc cgccctcggc gtcgcggccg atgagcgcca gcacaccgct gtagtagccg
     8821 cggccgtgcg gctcgaatct gcggacgacc ttggccgcgc tctccagcgg actgccggtc
     8881 accgtcggcg cgaacagggt ctcccgcagg acgtcgcgga cgtcggcggt cgcccggccc
     8941 tccacgacgt actccgtgtg cgccagaccg gccatctcga cgagccgcgg acccttcgcc
     9001 cggaccccga agtcgctgac ccgggccatc atcttcagtt cctcgtcgac gaccatcgac
     9061 agctcgtcgg cctccttggc gtcggccagg aaggacagca ccccggtcag cgtggggcct
     9121 tgcgccgggt accggtaggt gccgctgatc gggttcatga ccgcgacccc gtccgacagg
     9181 ctcagatgcc gctccggggt cgcgccgacg aacgtcctgc cgcccacgtg caccacgaag
     9241 gtccagtacg ccccccgctc ccgctccagc agacggcgga agaccgccag ggccagcagc
     9301 ggcgagtagt cggagatgtc cgcgacgaag gtccgtctga ccacgaagtt ggcgccctcg
     9361 ccgttgccga tctcctcctc gacgacgcgg cggaccaggt ccgcgtacgc cgcgtcgtcg
     9421 atgtcgaagc cacctccggc cagatcgatc cgggcgtccg gcaactgcgc cagtgcctcg
     9481 gagaccggga gcgactcctg cgtacggacc cgcagcgtga tcaccggggc cccgtcgtcc
     9541 accgcctcga agccgcgctc ggagagctgc cggtagggga tcacgaccag tacctcgtgc
     9601 tcggggcgcc cggtgcccgg gtcccggccg gcggtgtcgc tccgcagcgg gatgtcggcc
     9661 aggcgggcca cctccgccga gtcgccgacc agcacctcga tccggtccgc gggtccggtc
     9721 cgcggccggt gcagcaacgc gaactccggg tgcggcgccg ccaggatccg cccgagcggc
     9781 gacgcggcgt acgggtcgtc gcgcactgcc tgcgtcacgg cgccgcctcc tgtcggacgt
     9841 gtccgccgtc cggaaccggg gagagccggt ccaggacggc cgcggtcgtc agcgtcatcg
     9901 cgcaccgctc ggccgcgtag cgcagggcga accggtggtg ctcgggtgtg aagtcggcga
     9961 cggcgtcggc gacgaggaac gactggatgt ccgaggcgaa ggcgtccgcg ctggtcatga
    10021 ggattccgac atgggcgtac acaccgcaca cgatcagctg gtcgcgcccc cattcccgca
    10081 tggacgccag gagccgggtg cggtggaagg cgctgtagcg ccacttggtc agcaccaggt
    10141 cgcccgtacc gggccggatc gggtcgacca cctcacggtg ctcctgcgcg acggtcatcc
    10201 cggaacccca gaagtcccgc agcagaccgc gctgttgctc cgtcataccg ccgggctggg
    10261 tggtgtaggc gaccgggacg ccgagcgccg cgcaccgctc ccgcagggcg gtgacatggt
    10321 cgaccagttc cgtcaccggc gaccggccgg cggggaaggg ctcgacgaag tacttctgca
    10381 tgtcatggat cagcaggacc gcccgccgcg ggtccaccgt ccaccgggcg gtgttccgcg
    10441 gcaactcgtc ctcggtcggc atcgggtagg ccgtgatggc cgggattccc atacgggaac
    10501 tcctctctgg gagccggtgc ggccccgggg acggtgggat gcggactcgc accccacccc
    10561 gcgcccccgg ggcctctgtg atggatcgcc gcggcgtcgc gacaccgcct cggagacgac
    10621 ggccggcgaa ggcgccgtgc gggacttcgg aaagaccccc ctagtgaccg agcgccgcgc
    10681 cgccgtcgac ggtcaggtcc tgcatcgtga tgtgggcggc ccggtcggag agcaggaaca
    10741 ccaccgcgtc ggcgatgtgc tggggccgtg cgatgcgccc caacgggatg ccgacacgga
    10801 acgcggacgc gacgccgtcg atcgacgccc gctcggcgcc ctcgtcccag agcgcggtca
    10861 gcatcggggt gtcggtggag ccgggggcca ccacgttgca gcggataccg aaacgcgaca
    10921 cctccaggcc cagacacttg gtgaacatgg tcgaggcggc cttggacgcc gcgtacgcgg
    10981 ccatgtccac ccggggggtg cc
//
