LOCUS       NZ_KQ257855            10405 bp    DNA     linear   CON 02-JAN-2025
DEFINITION  Streptomyces stelliscabiei strain P3825 scf_652_34.1, whole genome
            shotgun sequence.
ACCESSION   NZ_KQ257855
VERSION     NZ_KQ257855.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces stelliscabiei
  ORGANISM  Streptomyces stelliscabiei
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 155366)
  AUTHORS   Harrison,J., Sapp,M., Thwaites,R. and Studholme,D.J.
  TITLE     Genome sequencing of plant-pathogenic Streptomyces species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 155366)
  AUTHORS   Harrison,J., Sapp,M., Thwaites,R. and Studholme,D.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2014) Biosciences, University of Exeter, Geoffrey
            Pope Building, Stocker Road, Exeter, Devon EX4 4QD, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Coverage                   :: 140.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001189035.1-RS_2025_01_02
            Annotation Date                   :: 01/02/2025 01:49:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,536
            CDSs (total)                      :: 9,455
            Genes (coding)                    :: 8,973
            CDSs (with protein)               :: 8,973
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 482
            CDSs (without protein)            :: 482
            Pseudo Genes (ambiguous residues) :: 0 of 482
            Pseudo Genes (frameshifted)       :: 129 of 482
            Pseudo Genes (incomplete)         :: 407 of 482
            Pseudo Genes (internal stop)      :: 20 of 482
            Pseudo Genes (multiple problems)  :: 69 of 482
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:19:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 84006
            Orig. end                         :: 94411
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KQ257855.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    70..84
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.71"
                     /tool="antismash"
     misc_feature    complement(184..198)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     gene            complement(559..1413)
                     /locus_tag="IQ64_RS09520"
                     /old_locus_tag="IQ64_10035"
     CDS             complement(559..1413)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338057.1"
                     /locus_tag="IQ64_RS09520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_10035"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_046919072.1"
                     /transl_table=11
                     /translation="MNSVLGRRTRTLAATTAAAGLLLVAGCGSEGDGGSGGTKTAAGGV
                     ELVKAGQLTTCTHLPYPPFQSEIDGKVQGFDVSLIDLVAEDLGVKQVVVDQPFENFKTG
                     GSLNAGQCDLAAAGMTITEERKKNVDFSDPYFEATQAVLADKTAGIASFADLKGKKVGT
                     QAQTTGEDYAKSKGIDSVSFESSDAVLNGLRTKQVDAVIIDYPVVQGWLKDKANADAFE
                     VADQINTGEQYGFTVKKGNTKLLAAIDEALAKAKSDGTYKKLYEQWIGPYDESAASASP
                     SAS"
     misc_feature    complement(1561..1577)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.26"
                     /tool="antismash"
     gene            1588..2688
                     /locus_tag="IQ64_RS09525"
                     /old_locus_tag="IQ64_10040"
     CDS             1588..2688
                     /EC_number="2.6.1.-"
                     /NRPS_PKS="Domain: Aminotran_5 (18-297). E-value: 2.1e-26.
                     Score: 84.6. Matches aSDomain:
                     nrpspksdomains_IQ64_RS09525_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477727.1"
                     /locus_tag="IQ64_RS09525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_10040"
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="WP_046919073.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.4e-21, bitscore:
                     70.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAAHFASIEDRVARLLSTTQDVVITQGEALLPLE
                     GAIRGAAGPGTTALNVITGPYGQTFGNWLRDCGATVIDLAVPFHTAVTAARIREAFAAH
                     PAIDFVSLVHAEAATGNTNPVAEIGEAVRAQGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAISVSERAWARMAANPRAPRRSYLSLLDWKERWIDGGRRALLHAP
                     AQLEMLALEACLQRIETEGLDTVMSRHASAAAATRAGALALGGGLEPYVHEAADAAPVA
                     TTLRAPAGADAAELVAEALAADPALPLAAGGGALASEVIRVNHYGADATPQVVHAALAG
                     LGAALAEHGRTVDPEGARRAAAQAWA"
     aSDomain        1642..2478
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IQ64_RS09525_Aminotran_5.1"
                     /evalue="2.10E-26"
                     /label="IQ64_RS09525_Aminotran_5.1"
                     /locus_tag="IQ64_RS09525"
                     /protein_end="297"
                     /protein_start="18"
                     /score="84.6"
                     /tool="antismash"
                     /translation="IEDRVARLLSTTQDVVITQGEALLPLEGAIRGAAGPGTTALNVIT
                     GPYGQTFGNWLRDCGATVIDLAVPFHTAVTAARIREAFAAHPAIDFVSLVHAEAATGNT
                     NPVAEIGEAVRAQGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSAISV
                     SERAWARMAANPRAPRRSYLSLLDWKERWIDGGRRALLHAPAQLEMLALEACLQRIETE
                     GLDTVMSRHASAAAATRAGALALGGGLEPYVHEAADAAPVATTLRAPAGADAAELVA"
     misc_feature    2874..2889
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(2910..2924)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.47"
                     /tool="antismash"
     misc_feature    3008..3020
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            3045..3596
                     /gene="ectA"
                     /locus_tag="IQ64_RS09530"
                     /old_locus_tag="IQ64_10045"
     CDS             3045..3596
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987798.1"
                     /locus_tag="IQ64_RS09530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_10045"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_225966655.1"
                     /transl_table=11
                     /translation="MTAAQADLHTQPLEAPQKIAEGIRIDRPSVVDGAALWRIAGDSGA
                     LDLNSSYSYLLWCRDFADTSVVARDDSGEPVGFVTGYVRPERPDTLLVWQVAVDEAHRG
                     LGLAAALLDGLTERVARRHPLTTVETTITPGNTASERLFAGYAARHGADIERTVLFEAG
                     DFPDGPHPPEMLHRIGPLSF"
     gene            3662..4933
                     /gene="ectB"
                     /locus_tag="IQ64_RS09535"
                     /old_locus_tag="IQ64_10050"
     CDS             3662..4933
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 6.8e-73.
                     Score: 237.5. Matches aSDomain:
                     nrpspksdomains_IQ64_RS09535_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="IQ64_RS09535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_10050"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_050398313.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.8e-79, bitscore:
                     258.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMFDEDGRTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRAFLESFQNLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALAALCERQDMLLIVDDIQMGCGRTGAFFSFEESGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTATAALEAYWADGSAMEKQTRARGEQVEEAL
                     ISITEENLADVKEYRGRGLVWGLEFHDKARAGRVAHRAFELGLLIETSGPESEVVKLLP
                     ALTITPEELDEGLRVLARAVRETV"
     aSDomain        3746..4759
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IQ64_RS09535_Aminotran_3.1"
                     /evalue="6.80E-73"
                     /label="IQ64_RS09535_Aminotran_3.1"
                     /locus_tag="IQ64_RS09535"
                     /protein_end="366"
                     /protein_start="28"
                     /score="237.5"
                     /tool="antismash"
                     /translation="FDRAQGSRMFDEDGRTYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRAFLESFQNLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALAALCERQDMLLIVDDIQMGCGR
                     TGAFFSFEESGITPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TATAALEAYWADGSAMEKQTRARGEQVEEALISITEENLADVKEYRGRGLVWGLEFH"
     gene            5001..5405
                     /locus_tag="IQ64_RS09540"
                     /old_locus_tag="IQ64_10055"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="IQ64_RS09540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_10055"
                     /product="ectoine synthase"
                     /protein_id="WP_046920322.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.2e-55, bitscore:
                     180.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKSASGTWESKRIVLARERVGFSLHETVLY
                     AGTETSMWYANHVEAVVCVEGEAELTDQETGRSYTITPGTMYLLDGHERHTLRVKEDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5413..6327
                     /gene="thpD"
                     /locus_tag="IQ64_RS09545"
                     /old_locus_tag="IQ64_10060"
     CDS             5413..6327
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="IQ64_RS09545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_10060"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_179860156.1"
                     /sec_met_domain="PhyH (E-value: 3.1e-42, bitscore: 138.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTTMNTATTVPDLYPSRGATEVSVPRQDPVVWGAPTTPGPVS
                     LTELQAYERDGFLPVEQLIAPDEVAVYRQELERLVADPAIRADERSIVEPQSQEIRSVF
                     EVHRISEIFARLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHA
                     EDGLPNMRTVSVSIALTENHDTNGGLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGT
                     PSDEALTAMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTA
                     VEPFSAPVRRPEFIGARDFTPVR"
     assembly_gap    6403..6416
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6417..7440)
                     /locus_tag="IQ64_RS45470"
                     /old_locus_tag="IQ64_10065"
     CDS             complement(<6417..7440)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477735.1"
                     /locus_tag="IQ64_RS45470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_10065"
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_078907190.1"
                     /transl_table=11
                     /translation="MTNDHDGVRELLAAWAVGALPPGDRRTVPLHLAGCESCAAEAERL
                     RDTVRLLDGGPVPARDGADAPGTGADGVLALALRSRRPRAAEIAHHAAPYAAAVAGLQA
                     LVPEVDGRWGTPVVHDWDAHATVAHLVAADEHLAVHLGLDPPLPASHIPEGMPAGDAWA
                     RRTADVIAHEHGRTPAETVATWAAQAAALLATPEAADPELAARPVSVMGLRLPVADHFV
                     VRAFEAWIHTDDIGRALGLPVPPPPDEHLWSLVRLAVRILGLALHDAAPVLFEVTGPTG
                     TSWILGDDTGPVRAELTLDPVDFCLLVGGRHTPGGVPRGITGDAAAARNVLDRAASLAW
                     L"
     gene            complement(7517..8125)
                     /locus_tag="IQ64_RS09555"
                     /old_locus_tag="IQ64_10070"
     CDS             complement(7517..8125)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477737.1"
                     /locus_tag="IQ64_RS09555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_10070"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_046913311.1"
                     /transl_table=11
                     /translation="MVVPVEPTLEQPSFDVELHRRLVYGDESALREAYAVYGGLVRRVA
                     ARVTRSPAAAEDVAQEVFAQLWSRPYGFDARRGSLRTWLSMLAHRRAVDWVRDEARHRR
                     DARADDSALHAVPDPRPGPDEAVVDRERSLLLHTALAGLPRPQREVVHLAYFAGRTYRQ
                     AAVELGIPEGTAKTRLRSALRTLARSLADPPDPAAVRGA"
     gene            complement(8182..9231)
                     /locus_tag="IQ64_RS09560"
                     /old_locus_tag="IQ64_10075"
     CDS             complement(8182..9231)
                     /NRPS_PKS="Domain: Aminotran_5 (16-343). E-value: 3.7e-23.
                     Score: 73.9. Matches aSDomain:
                     nrpspksdomains_IQ64_RS09560_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477739.1"
                     /locus_tag="IQ64_RS09560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_10075"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_046913312.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.8e-18, bitscore:
                     59.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRTEFAPKNTYLNTASTGLLPARTVDAMKAGVDSVAAG
                     QPQDMFADVEAARAAFGRIVGVPDSRVAAGASVAVYSGLVAASLPEGAEVLTAEADFTS
                     LVNPFHVRGDLKVRQVPLERVAESVRPGTALVAVSAAQSADGRIADLPAIREAALTHGA
                     RTYVDASQSAGWLPIEADAYDYVVSVAFKWLLCPRGVTFLVVPEDLGGLTPLFAGWVAG
                     QEPWDSCYGPVEELARSARRFDESPSLFSYAGARRSLALIEELGVPRVRDHDLALAGRF
                     RAGLAGLGHEPVPAPGSAIVSVPGLGHQQAELSRAGVEVSDRAGHLRAAFHLYNTPADV
                     DRLLDVLSG"
     aSDomain        complement(8203..9183)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IQ64_RS09560_Aminotran_5.1"
                     /evalue="3.70E-23"
                     /label="IQ64_RS09560_Aminotran_5.1"
                     /locus_tag="IQ64_RS09560"
                     /protein_end="343"
                     /protein_start="16"
                     /score="73.9"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPARTVDAMKAGVDSVAAGQPQDMFADVEAARAAF
                     GRIVGVPDSRVAAGASVAVYSGLVAASLPEGAEVLTAEADFTSLVNPFHVRGDLKVRQV
                     PLERVAESVRPGTALVAVSAAQSADGRIADLPAIREAALTHGARTYVDASQSAGWLPIE
                     ADAYDYVVSVAFKWLLCPRGVTFLVVPEDLGGLTPLFAGWVAGQEPWDSCYGPVEELAR
                     SARRFDESPSLFSYAGARRSLALIEELGVPRVRDHDLALAGRFRAGLAGLGHEPVPAPG
                     SAIVSVPGLGHQQAELSRAGVEVSDRAGHLRAAFHLYNTPADVDRL"
     gene            complement(9342..10058)
                     /locus_tag="IQ64_RS09565"
                     /old_locus_tag="IQ64_10080"
     CDS             complement(9342..10058)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020114333.1"
                     /locus_tag="IQ64_RS09565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_10080"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_046913313.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDGVEVVHRSYELDP
                     GRAKDDIQPVLTMLSKKYGMSEAQAQAGEHNLREQAGAEGLAYRAEGRDHGSTFDMHRL
                     LHFAKEQGRQSELLQAFYRANFAEERSVYADADEYLVELAVAAGLEESAVRTVLTDSGA
                     YADAVRADEREAAELGANGVPFFVLDRTYGVSGAQPAEVFERALTQAWGERPAPLKVVA
                     AEGADACGPDGCAVPQ"
ORIGIN
        1 gcgcgggtga gaagcccagc gaacgggccg cctccagctg ccccttggga accgcctgga
       61 tgcccgcgcg gatcgtctcc gccatgtacg cggcggccac cagcccgagg gcgagggcga
      121 ccttgccgta ggtgccgccg gggatctccg tgccggggaa ggccagcggg acggcgacgc
      181 cgatgaagat gaagatcagc agggcgggca ggccccggaa gatctcgatg tagacaccgg
      241 cgaaccagcg gtacgggccg accgacgaca gccgcatcag cgcgatgacc atgccgagga
      301 cgagcccgaa cacgaagccg gacagcgtgt acagcacggt gttcttgagc gcgagcgtga
      361 tgacgtcggg gaacatctgc tcggcgatgt cccactgggc gaactggttc ttcagccggg
      421 cccagtccgc ggacgccgcg aaggcgacga ccacggcgac gaacacgacg tactgcgcgc
      481 cccgggacag ggcgcgcctg cgactgcggc tcaggccctt cctcttcggc tggagcgggg
      541 tgtccgtgtc ggcctggctc atgaggcgga cggggaggcg gaggcggcgc tctcgtcgta
      601 cgggccgatc cactgctcgt acagcttctt gtacgtgccg tcggacttgg ccttcgccag
      661 cgcctcgtcg atggcggcga ggagcttggt gttgcccttc ttcaccgtga agccgtactg
      721 ctcaccggtg ttgatctggt cggcgacctc gaaggcgtcg gcgttcgcct tgtccttgag
      781 ccagccctgg acgaccgggt agtcgatgat cacggcgtcg acctgcttcg tgcgcaggcc
      841 gttgaggacg gcgtcggagg actcgaagga gaccgaatcg atgcccttgc tcttggcgta
      901 gtcctcgccc gtggtctgcg cctgggtacc gaccttcttg cccttgaggt cggcgaagga
      961 ggcgatgccg gcggtcttgt cggcgaggac ggcctgggtg gcctcgaagt acgggtcgga
     1021 gaagtcgacg ttcttcttgc gctcttcggt gatcgtcata ccggcggcgg cgaggtcgca
     1081 ctggccggca ttgagggatc caccggtctt gaagttctcg aagggctggt cgacgactac
     1141 ctgcttcaca ccgaggtcct cggcgacgag gtcgatcagc gacacgtcga agccctgcac
     1201 cttgccgtcg atctccgact ggaagggcgg gtacggcagg tgcgtgcagg tcgtgagctg
     1261 accggccttc accagctcga ccccgccggc cgcggtcttg gtgccgccgc tgccgccgtc
     1321 accctccgag ccacagccgg ccacgagcag caggccggcc gcggcggtgg tggcggccag
     1381 ggtgcgggtc cggcgtccga ggaccgagtt cacggggaga cctcctgtaa gggaacttga
     1441 gacaaccgat tataaggaga tgtttgactt ccccaaacca atccgatggg taccgggccg
     1501 cttgacctcg gatgtcgccg gtgagggggg cgagggtgcc gtcggtaccc tcgacaccat
     1561 ctaccccata ggtgaagaga gcaccccgtg acacacccgt ttctggatct ggccccgctg
     1621 agcgcggcac acttcgcgtc gatcgaggac cgggtggccc gactgctctc caccacgcag
     1681 gacgtcgtga tcacccaggg cgaggcgctg ctgccgctgg agggcgcgat ccgcggggcc
     1741 gccgggcccg gcacgaccgc gctgaacgtg atcaccggcc cgtacgggca gaccttcggc
     1801 aactggctgc gggactgcgg ggcgaccgtg atcgacctgg ccgtcccctt ccacacggcg
     1861 gtcacggccg cgcggatccg ggaggccttc gccgcgcacc cggcgatcga cttcgtctca
     1921 ctggtccacg ccgaggcggc gaccggcaac accaaccccg tggcggagat cggcgaggcc
     1981 gtccgggcgc agggcgccct gttctacctg gacgcggtcg cctccgtcgg cgccgagccg
     2041 gtactgccgg acgcgtgggg tgtcgacctg tgcgtcatcg gcgcgcagaa ggccatgggc
     2101 gggccggccg gggtgtcggc gatctccgtg agcgagcggg cgtgggcgcg gatggcggcc
     2161 aacccgcgcg ccccgcgccg ctcctacctc tccctgctgg actggaagga gcgctggatc
     2221 gacggcggcc gccgcgcgct cctgcacgca ccggcccagc tggagatgct ggcgctggag
     2281 gcctgtcttc agcgcatcga gacggagggg ctcgacacgg tgatgtcccg gcacgcgtcc
     2341 gccgccgcgg cgacccgggc gggggcgctc gcgctgggcg gcggtctgga gccctacgtc
     2401 cacgaggctg ccgacgcggc gccggtggcc acgaccctgc gggcgcccgc cggggccgac
     2461 gcggcggagc tggtcgccga ggcgctcgcc gcggaccccg ccctgccgct ggccgcgggc
     2521 ggcggcgccc tcgcctcgga ggtgatccgc gtgaaccact acggcgccga cgcgaccccg
     2581 caggtcgtgc acgcggccct cgcggggctg ggggcggcgc tggccgagca cggcaggacg
     2641 gtcgatccgg aaggggcccg tcgcgcggcg gcgcaggcct gggcgtagag cggcgggcag
     2701 ctgggcgtga aaaggcccgc cctgagaatt tccgatatct tccacggggt aattcagtga
     2761 attcagcggg tagaaccccg tattcttctg cccgctctcc ccttccctaa acgcaaagat
     2821 ttcgcgaacg cggcggggcg taattcgagc agatctcgtg agggttgcac acgtgtgacc
     2881 gactccacag cgcgcccttt tcgtacggta atttccggac gaaaagcatc agatccacct
     2941 cggttcgacg gagcactcgc gcccgcgtga taacacgcgc tcggcgcata cgtaaccccg
     3001 cggggcgatg caattcgaaa ttccgctggg taaattcaag ccgtatgacc gccgcacaag
     3061 cagacctgca cacccaaccc ctggaagccc ctcagaaaat cgcggaaggg attcggatcg
     3121 accgcccgag cgtggtggac ggcgcggcgc tgtggcgcat cgccggggac tccggagccc
     3181 tcgacctgaa ctcctcgtac agctatctgc tgtggtgccg ggacttcgcc gacacctcgg
     3241 tggtggcgcg ggacgactcc ggagagccgg tcgggttcgt gaccgggtac gtgcggcccg
     3301 agcggccgga caccctgctc gtgtggcagg tggccgtcga cgaggcgcac cgggggctcg
     3361 gcctcgcggc ggcgctgctg gacggactga ccgaacgggt cgcacgacgg caccccctga
     3421 ccaccgtcga gaccaccatc acgccgggca acaccgcctc cgagcgtctg ttcgccgggt
     3481 acgccgcccg tcatggcgcg gacatcgagc gcacggtgct cttcgaggcg ggggacttcc
     3541 cggacggccc gcacccgccc gagatgctcc accgcatcgg accgctgtcc ttctgagcac
     3601 cacccgcact ccccctcccc ccttcctctc cctcccccca cgcaccgagg agcgattcga
     3661 cgtgaccatc acccagcccg acctgagcgt cttcgagacc ctggagtccg aggtgcgcag
     3721 ctactgccgc ggctggccca ccgtcttcga ccgcgcgcag ggcagccgca tgttcgacga
     3781 ggacggccgc acgtacctgg acttcttcgc cggcgccggt tcactcaact acggacacaa
     3841 caaccccgta ctcaaacgcg cgctcatcga ctacctggag cgggacggcg tcacccacgg
     3901 gctcgacatg tcgacgaccg ccaagcgggc gttcctggag tccttccaga acctggtgct
     3961 gcggccgcgc gacctgccgt acaaggtgat gttcccgggg ccgacgggca ccaacgcggt
     4021 ggagtcggcg ctgaagctgg cgcggaaggt gaagggccgg gagtcggtcg tgtccttcac
     4081 gaacgccttc cacggcatgt cgctcggctc gctggccgtg accggcaacg ccttcaagcg
     4141 ggccggggcg ggcatcccgc tggtgcacgg caccccgatg cccttcgaca actacttcga
     4201 cggcacggtc gaggacttcc tgtggttcga gcgactgctg gaggaccagg gctccgggct
     4261 gaacaagccc gccgcggtga tcgtggagac cgtgcagggc gagggcggca tcaacgtcgc
     4321 ccgcccggag tggctgcggg cgctggccgc gctgtgcgag cggcaggaca tgctgctgat
     4381 cgtcgacgac atccagatgg gctgcggtcg cacgggtgcc ttcttctcgt tcgaggagtc
     4441 gggcatcacc cccgacatcg tgacggtctc caagtccatc agcggctacg ggctgccgat
     4501 gtcgctgtgc ctgttcaagc cggagctgga catctgggag ccgggcgagc acaacggcac
     4561 cttccgcggc aacaacccgg cgttcgtgac ggcgacggcc gccctggagg cgtactgggc
     4621 ggacggctcc gccatggaga agcagacccg ggcgcgcggt gagcaggtcg aggaggcgct
     4681 gatctcgatc accgaggaga acctcgccga cgtgaaggag taccggggcc gcggcctggt
     4741 gtggggcctg gagttccacg acaaggcccg cgcgggacgg gtcgcccacc gggccttcga
     4801 gctggggctg ctgatcgaga cgtccggccc ggagagcgag gtcgtcaaac tgctgcccgc
     4861 tctcaccatc acccccgagg aactggacga ggggctgcgc gtcctcgccc gggccgtgcg
     4921 ggagacggtc tgagaccgca ccgggtcacc ccgagcacct gaagcacctg aagcacctaa
     4981 ctaggaggca tcgcaacacc gtgatcgtcc gttcgttcaa ggacatcgaa ggaaccgacc
     5041 ggcatgtgaa gtcggcgtcc ggcacctggg agagcaagcg gatcgtgctc gcccgggagc
     5101 gggtcggctt ctccctccac gagacggtcc tgtacgcggg gaccgagacg tcgatgtggt
     5161 acgcgaacca cgtcgaggcc gtcgtgtgcg tcgagggcga ggccgagctc accgaccagg
     5221 agaccgggcg gagttacacg atcacgcccg gcaccatgta cctcctcgac gggcacgagc
     5281 ggcacacgct gcgggtgaag gaggacttcc gctgcgtctg cgtgttcaac ccgcccgtga
     5341 ccggccggga ggaccacgac gagaacggcg tctacccgct gctcaccgag cccgaggagg
     5401 tgtgacatca ccatgaccac gaccaccacg atgaacactg ccaccaccgt gcccgacctc
     5461 taccccagcc gtggggccac cgaggtgtcg gtcccgcgtc aggacccggt cgtctggggc
     5521 gcccccacca cgcccggccc ggtctccctc accgaactcc aggcctacga gcgcgacggc
     5581 ttcctgcccg tcgagcagct catcgcgccg gacgaggtcg ccgtctaccg gcaggagctg
     5641 gagcggctcg tcgccgatcc ggcgatccgc gccgacgagc gctcgatcgt cgagccgcag
     5701 tcgcaggaga tccggtcggt cttcgaggtg caccggatca gcgagatctt cgcccggctg
     5761 gtgcgcgacg agcgggtcgt cggacgggcc cggcagatcc tcggctcgga cgtctacgtc
     5821 caccagtcgc ggatcaacgt caagccgggg ttcggcgcct ccggcttcta ctggcactcc
     5881 gacttcgaga cctggcacgc cgaggacggt ctgccgaaca tgcggacggt gtccgtctcg
     5941 atcgccctga ccgagaacca cgacaccaac ggcggcctga tgatcatgcc ggggtcgcac
     6001 cggacgttcc tcggctgtgc gggggccacc cccaaggaca actacaagaa gtcgctgcag
     6061 atgcaggacg ccggcacccc gtccgacgag gcgctgacgg ccatggcctc cgagtacggc
     6121 atcaagctgt tcacgggcaa ggccggttcg gcgacctggt tcgactgcaa ctgcatgcac
     6181 ggctccggcg acaacatcac gccgttcccg cgcagcaacg tcttcatcgt cttcaacagc
     6241 gtggagaaca cggcggtcga gccgttctcg gcgccggtac ggcggcccga gttcatcggc
     6301 gcgcgagact tcaccccggt gcgctgagca ccggatccgg gcccgtgcgg tgacctcacc
     6361 gtcacccggg ccgggccggg cggccgctgc ccctgagcgg ccnnnnnnnn nnnnnnacag
     6421 ccaggccagt gacgccgccc tgtccagcac gttccgggcg gcggccgcgt cgcccgtgat
     6481 gcctctgggg acgccccccg gggtgtgacg gccaccgacc aggaggcaga agtccacggg
     6541 atccagggtg agttcggccc gtacgggacc ggtgtcgtca ccgaggatcc agctggtgcc
     6601 ggtggggccg gtgacctcga agaggacggg ggccgcgtcg tggagggcca gaccgaggat
     6661 gcggaccgcc aggcggacca gggaccagag gtgttcgtcc gggggcgggg ggacggggag
     6721 gccgagcgcg cggccgatgt cgtccgtgtg gatccatgcc tcgaaggcgc ggaccacgaa
     6781 gtggtcggcg accggcagcc gcaggcccat caccgagacg gggcgggcgg cgagttcggg
     6841 gtcggcggcc tcgggggtgg ccagcagcgc ggcggcctgc gcggcccagg tggcgaccgt
     6901 ctccgccggg gtgcgcccgt gctcgtgggc gatgacgtcg gcggtgcgcc gggcccaggc
     6961 atccccggcg ggcatcccct ccgggatgtg cgaggcgggc aggggcgggt ccaggccgag
     7021 gtgtacggcg aggtgctcgt cggcggcgac gaggtgcgcg accgtggcgt gcgcgtccca
     7081 gtcgtgcacc accggtgtgc cccagcggcc gtccacctcc ggtaccagcg cctggagtcc
     7141 ggccacggcg gcggcgtagg gggcggcgtg gtgggcgatc tcggcggcgc gggggcggcg
     7201 ggagcgcagg gcgagcgcga ggacgccgtc ggcgccggtg cccggggcgt cggcaccgtc
     7261 acgggcgggg accggcccgc cgtccagcag gcgtacggtg tcgcggagcc gttcggcctc
     7321 cgccgcgcac gactcgcagc cggccaggtg cagggggacc gtccgccggt cgccgggggg
     7381 cagcgcgccg accgcccagg cggccagcag ctcccgtacc ccgtcgtggt cgttggtcat
     7441 cgcgcgccgc cctttcgccc tgccccgtcc acgatacgca cgaccggcgc ggtcccggcg
     7501 gtcacgtacg cccccatcac gcgcccctca ccgccgccgg gtccggtggg tccgcgaggg
     7561 agcgggccag ggtgcgcagg gccgagcgga gccgggtctt cgcggtgccc tcgggtatgc
     7621 ccagttcgac ggcggcctgg cggtaggtgc ggcccgcgaa gtaggcgagg tggaccacct
     7681 cccgctgggg gcgcgggagt ccggcgaggg cggtgtgcag cagcagggag cgctcgcggt
     7741 cgacgaccgc ctcgtccggg ccgggccggg ggtcggggac ggcgtgcagc gcggagtcgt
     7801 ccgcgcgggc gtccctgcgg tgccgggcct cgtcgcgcac ccagtccacg gcccgccggt
     7861 gggcgagcat ggagagccag gtgcgcagcg agccccggcg cgcgtcgaag ccgtacggcc
     7921 tgctccacag ctgggcgaag acctcctgcg ccacgtcctc ggcggctgcc gggctgcggg
     7981 tgacccgggc ggccacccgc cggacgagtc ccccgtacac cgcgtacgcc tcacgcagcg
     8041 cggactcgtc cccgtacacc agccgccgat gcaactccac atcgaaggac ggctgttcga
     8101 gcgtcggctc caccggcacc accacctcgt gaagccttcc tagcgccctc gcccggtccc
     8161 gcgccagtgg gtctcgtgaa ctcagccgga caggacgtcc aggaggcggt cgacatccgc
     8221 gggcgtgttg tacaggtgga aggcggcccg cagatggccc gcgcgatcgg agacctcgac
     8281 gccggcccgg ctcagttccg cctgctggtg gccgagtccg ggcaccgaga cgatcgccga
     8341 tccgggcgcc ggaacgggct cgtggccgag gccggcgagg cccgcgcgga accggccggc
     8401 cagcgccagg tcgtggtccc gcacgcgggg gacgcccaat tcctcgatca gcgccaggga
     8461 ccggcgtgcg ccggcgtacg agaacaggct cgggctctcg tcgaaccggc gggcggagcg
     8521 ggcgagttcc tcgaccgggc cgtagcagct gtcccacggt tcctgtcccg cgacccagcc
     8581 ggcgaagagg ggggtgaggc cgccgaggtc ctccgggacg accaggaagg tcactccgcg
     8641 cggacagagg agccacttga aggcgacgga gacgacgtag tcgtacgcgt ccgcctcgat
     8701 cgggagccag ccggcggact gcgacgcgtc gacgtacgtg cgcgcgccgt gggtcagcgc
     8761 ggcctcccgg atcgcgggca ggtcggcgat ccggccgtct gcggactggg cggcgctgac
     8821 cgcgacgagc gccgtgccgg ggcggaccga ctccgcgacg cgctccagcg ggacctggcg
     8881 caccttgagg tcgccgcgca cgtggaaggg gttcacgagg gaggtgaagt cggcctcggc
     8941 ggtcagcacc tcggcgccct cgggcagcga ggcggcgacc agtccgctgt agacggcgac
     9001 cgaggcgccg gccgccacac ggctgtccgg caccccgacg atccgcccga aggccgcccg
     9061 cgcggcctcc acgtcggcga acatgtcctg cggctgcccg gccgcgaccg agtccacacc
     9121 ggccttcatc gcgtcgaccg tgcgggccgg cagcagtccc gtactcgcgg tgttcagata
     9181 ggtgttcttc ggggcgaact cggtccggac gaggctctcg aaggtctcca tggctccact
     9241 gtgggccccc gcgcatccca cgtccatgga gatgttctgc gcgatttccc caagcgatac
     9301 ttatggatgg ccgctgacct gcgccgcagg cctcaccgcg gctactgcgg cacggcacat
     9361 ccgtcgggtc cgcaggcgtc cgcgccctcg gccgcgacga ccttcagggg cgcgggccgc
     9421 tcgccccacg cctgcgtgag cgcccgctcg aacacctcgg cgggctgggc gccggacacc
     9481 ccgtacgtgc ggtcgagcac gaagaacggc accccgttcg cccccagctc cgccgcctcg
     9541 cgctcgtccg cgcggacggc gtcggcgtac gcgccggagt cggtgagcac cgtgcggacc
     9601 gcactctcct ccagtcccgc cgccacggcc agctcgacca ggtactcgtc cgcgtcggcg
     9661 tacacggacc gctcctcggc gaagttcgcc cggtagaagg cctggagcag ctcgctctgg
     9721 cggccctgct ccttggcgaa gtgcagcagg cgatgcatgt cgaaggtgct gccgtggtca
     9781 cggccctcgg cccggtaggc gaggccctcg gcgccggcct gctcgcgcag gttgtgctcg
     9841 ccggcctggg cctgcgcctc gctcatcccg tacttcttgc tcagcatggt gaggaccggc
     9901 tggatgtcgt ccttggcgcg gcccgggtcg agctcgtagg agcggtgcac cacctcgacg
     9961 ccgtcgcggt gcgggaaggc ggcgagggcc ttctcgaagc gggccttgcc cacgtagcac
    10021 caggggcagg cgatgtcgct ccagatctcg acgcgcatgg tgtcagctct ctccaggtcg
    10081 tacgggtatg acggagacgg cccccgcctc caggattggt gaacgttcaa gcagctcggc
    10141 tcattcccgg ccacgccctt tccggtcacc gtgagggccc gaggcgcgcc tcagctgccg
    10201 tttctcaggt ccttgggcca gttcgggcgg aactcgatct cgtcgtaggt gaccacgcag
    10261 ccctcaccca tcggggactg ggtcatgaag ccgacgaggg cggcaccgct ctcgtagggg
    10321 tcggagaggg tgaagaggcg gacgaagatc cagcgcttgc cgtcgcgcga ggcgtggaag
    10381 gcgaaggcgc ggccggtgcg gctga
//
