LOCUS       NZ_KQ257854            10459 bp    DNA     linear   CON 02-JAN-2025
DEFINITION  Streptomyces stelliscabiei strain P3825 scf_652_32.1, whole genome
            shotgun sequence.
ACCESSION   NZ_KQ257854
VERSION     NZ_KQ257854.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces stelliscabiei
  ORGANISM  Streptomyces stelliscabiei
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1039497)
  AUTHORS   Harrison,J., Sapp,M., Thwaites,R. and Studholme,D.J.
  TITLE     Genome sequencing of plant-pathogenic Streptomyces species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1039497)
  AUTHORS   Harrison,J., Sapp,M., Thwaites,R. and Studholme,D.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2014) Biosciences, University of Exeter, Geoffrey
            Pope Building, Stocker Road, Exeter, Devon EX4 4QD, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Coverage                   :: 140.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001189035.1-RS_2025_01_02
            Annotation Date                   :: 01/02/2025 01:49:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,536
            CDSs (total)                      :: 9,455
            Genes (coding)                    :: 8,973
            CDSs (with protein)               :: 8,973
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 482
            CDSs (without protein)            :: 482
            Pseudo Genes (ambiguous residues) :: 0 of 482
            Pseudo Genes (frameshifted)       :: 129 of 482
            Pseudo Genes (incomplete)         :: 407 of 482
            Pseudo Genes (internal stop)      :: 20 of 482
            Pseudo Genes (multiple problems)  :: 69 of 482
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:19:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 364124
            Orig. end                         :: 374583
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KQ257854.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10459
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="2"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10459
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10459
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5459](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5459
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    994..1003
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: medium, score: 16.35"
                     /tool="antismash"
     misc_feature    995..1008
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.7"
                     /tool="antismash"
     misc_feature    complement(997..1010)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.05"
                     /tool="antismash"
     gene            1028..1267
                     /gene="chpG"
                     /locus_tag="IQ64_RS06070"
                     /old_locus_tag="IQ64_06385"
     CDS             1028..1267
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="IQ64_RS06070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06385"
                     /product="chaplin ChpG"
                     /protein_id="WP_005479502.1"
                     /transl_table=11
                     /translation="MSRIAKGLALTSVAAAAVAGTAGVATADSEAKGFAAHSPGVLSGN
                     VVQVPVHIPVNVCGNTINVIGLLNPSFGNKCFND"
     gene            1458..2303
                     /locus_tag="IQ64_RS06075"
                     /old_locus_tag="IQ64_06390"
     CDS             1458..2303
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003503.1"
                     /locus_tag="IQ64_RS06075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06390"
                     /product="hypothetical protein"
                     /protein_id="WP_046919149.1"
                     /transl_table=11
                     /translation="MRVLPARRLAVSALCATLLLGVTAPAALAADVESARERARSAAPV
                     PGADALQDQLKGLGDLGGVLKPVTDLLDAVLEADNGQLSADRAAELGKAVRDAIDRAKA
                     GAPVTSPAVPPAVPPVALPVAPPAPAVPPAPAVPPAATTPTVPSVPAAPSLPSVPTAPA
                     APVVPAAPQQAVTLPAFDRALVRPAGSVAANPVTDALTALQKAVDTLLKAVTSGDLAQV
                     LPAVNSVVAGLVQLIGSILLGSGLPAPVALPAVQSPAELPVTPSTDTLPVRPPAVPPVR
                     "
     gene            2488..2778
                     /locus_tag="IQ64_RS06080"
                     /old_locus_tag="IQ64_06395"
     CDS             2488..2778
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445910.1"
                     /locus_tag="IQ64_RS06080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06395"
                     /product="hypothetical protein"
                     /protein_id="WP_046919148.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLAIAGAAAPAFAYDATDVTPTSLNGAVNTLFK
                     GPIAPAPMQQQSNALDTENKDSVLHTVSGATKALNAPGGPSKLLGGLPLQG"
     gene            2977..3474
                     /locus_tag="IQ64_RS06085"
                     /old_locus_tag="IQ64_06400"
     CDS             2977..3474
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003505.1"
                     /locus_tag="IQ64_RS06085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06400"
                     /product="DUF5949 family protein"
                     /protein_id="WP_046919147.1"
                     /transl_table=11
                     /translation="MTSTSAETRPFRPADLGTLVVMAWSGEAPDGDMPYLLAYTLGDGR
                     NGPEGSTAAVADLLTGLGLSIGEKVVDGAAHPSLPVTLLVEAGQAVITLPQLNAQCPAP
                     PEWLAAVGTRGFAYLLFATRPWPEARPGMPVEPAALAAFAGDPETLTSAAHVLLPARSL
                     RG"
     gene            3474..4079
                     /locus_tag="IQ64_RS06090"
                     /old_locus_tag="IQ64_06405"
     CDS             3474..4079
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005479492.1"
                     /locus_tag="IQ64_RS06090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06405"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_046919146.1"
                     /transl_table=11
                     /translation="MAVPAGRPARTAGGGRAFAILLLLTGAAGLLASWVITLDKFKLLE
                     DPNFTPGCSLNPVVSCGNIMKSDQASAFGFPNPMLGLVAYGVVVCVGVSLLAGAVFPRW
                     YWLTFGAGALFGVGFVTWLQFESLYRINSLCLWCCLAWIATIVLFWYLLSFDVRNGFLP
                     APAWLSGFLVEFTWVLPVLHLGVIGMLVLTRWWDFWTS"
     gene            4400..4909
                     /locus_tag="IQ64_RS06095"
                     /old_locus_tag="IQ64_06410"
     CDS             4400..4909
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003507.1"
                     /locus_tag="IQ64_RS06095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06410"
                     /product="hypothetical protein"
                     /protein_id="WP_046919152.1"
                     /transl_table=11
                     /translation="MKPTTRGTLAAVVTGVAAAMGASAAPAVAAETVPVPLPLEGVEHS
                     LNVKAPKLSGELPLLMPGAPQGGPKYVEGRLLPASSLPQVPLGTALPGLKAETPLPRVV
                     GEGFERLGVDAPAADLRTLTPGLNVDTPLTAPDPHALGLPSLKDPQVAVLTPLLRTAPT
                     ANLGLV"
     gene            5001..5459
                     /locus_tag="IQ64_RS06100"
                     /old_locus_tag="IQ64_06415"
     CDS             5001..5459
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378445.1"
                     /locus_tag="IQ64_RS06100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06415"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_404819281.1"
                     /sec_met_domain="melC (E-value: 3.4e-46, bitscore: 152.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLFVSRVTVDPEAGRSRTRRNALRVLGASAVALAVAPVVAAAWPR
                     RSARPGEVDFDETFRGRRIRGVRADTGQTAYLGARTPGPGEWDVTVDGRPLHLMRRADG
                     TYLSMIDHYRSYPTALEATRAAVEELGTTQRLRVGETSEGAVGHGLHA"
     gene            5443..6309
                     /locus_tag="IQ64_RS06105"
                     /old_locus_tag="IQ64_06420"
     CDS             5443..6309
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005479487.1"
                     /locus_tag="IQ64_RS06105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06420"
                     /product="tyrosinase family protein"
                     /protein_id="WP_046919144.1"
                     /transl_table=11
                     /translation="MVYTRKDVSTLTRTERRRFVGALLKLKQRGEYDEFVRMHIDHYVS
                     DGDGGLRAAHMAPSFLPWHRRFVLELEKALRRIDDSVTVPYWDWTRDRTTTSVPWTADL
                     LGGNGRRSDLRVTTGPFAYGTGRWTITEGLTDIEYLTRDFGYPRNPVGLPTARDLDAAL
                     AESVYDKAPWNSVTRGGFRNRLEGWGQGTGNGSWLNHNRVHRWVGGHMVGGASVNDPVF
                     WLHHAFIDLQWTRWQRRHRGARYLPAKPPGKGDSQRGRIVARDERMPPWNVTPGALESI
                     GHVYRYG"
     gene            complement(6424..6711)
                     /locus_tag="IQ64_RS06110"
                     /old_locus_tag="IQ64_06425"
     CDS             complement(6424..6711)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115467.1"
                     /locus_tag="IQ64_RS06110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06425"
                     /product="chaplin"
                     /protein_id="WP_179860117.1"
                     /transl_table=11
                     /translation="MSRIAKAAVVTLGTSAVVLSGAGLAAADAGAEAKAIGSPGVLSGN
                     IIQVPVNVPVNLCGNTINVVGLLNPAFGNVCVNKSHDNHKGNPGPSGYGH"
     gene            complement(6979..7398)
                     /locus_tag="IQ64_RS06115"
                     /old_locus_tag="IQ64_06430"
     CDS             complement(6979..7398)
                     /EC_number="1.20.4.4"
                     /GO_function="GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019063522.1"
                     /locus_tag="IQ64_RS06115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06430"
                     /product="arsenate reductase ArsC"
                     /protein_id="WP_050398206.1"
                     /transl_table=11
                     /translation="MTSPLASVLFVCVHNAGRSQMAAGFLTHLAGDRIEVRSAGSLPAD
                     RVNPAAVEAMKEVGVDIAGAAPKVLTTEAVQASDYVITMGCGDACPVFPGKKYLDWALE
                     DPAGQGVEAVRPIRDGIRTRVEALIAEITENDTRS"
     misc_feature    7463..7481
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.38"
                     /tool="antismash"
     misc_feature    complement(7494..7506)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 18.92"
                     /tool="antismash"
     gene            7585..7884
                     /locus_tag="IQ64_RS06120"
                     /old_locus_tag="IQ64_06435"
     CDS             7585..7884
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005479483.1"
                     /locus_tag="IQ64_RS06120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06435"
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="WP_050398296.1"
                     /transl_table=11
                     /translation="MRDLIRVLADPLRLRIVTLLARETLCTTHLVEETGARQTNLSNHL
                     KVLREAGIVETEPCGRWTYYRLRPEALASLAGGFAELAEAARVTVENDVKRSCS"
     gene            8005..8811
                     /locus_tag="IQ64_RS06125"
                     /old_locus_tag="IQ64_06440"
     CDS             8005..8811
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015267 - channel activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944637.1"
                     /locus_tag="IQ64_RS06125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06440"
                     /product="aquaporin"
                     /protein_id="WP_079028211.1"
                     /transl_table=11
                     /translation="MGIDTVAVAPGRTPLGARVAAEFVGTAALVTVVVGSGIQATALTP
                     DVGVRLLANSLATVFGLGVLIALFGPVSGAHFNPVVTSAEWWTGRRRGGVRGREVAEAA
                     GATGLTAREAVAYVAAQTLGAIAGAVLADAMFAEPLVKWSTHERSAGHLLLAEVVATAG
                     LVLLVLGLARSDRLRFAPVAVASYIGSAYWFTSSTSFANPAVTVGRAFTDTFAGIAPGS
                     VAGFVGAQLVGGAIGLGLVAVIWPSSSRLAERVRRAAAPSVADRRV"
     tRNA            complement(8888..8970)
                     /anticodon=(pos:complement(373049..373051),aa:Arg,seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="IQ64_RS48450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Arg"
     gene            complement(8888..8970)
                     /locus_tag="IQ64_RS48450"
     gene            complement(9059..9511)
                     /locus_tag="IQ64_RS06130"
                     /old_locus_tag="IQ64_06445"
     CDS             complement(9059..9511)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IQ64_RS06130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="IQ64_06445"
                     /product="hypothetical protein"
                     /protein_id="WP_159026065.1"
                     /transl_table=11
                     /translation="MASEWIPAVVTGVSGALGVTFTWLAGAQGRTHAERMVTQSREAEQ
                     HTYLRRERRDAYFEALRVAVLDVRRLRYERSGKIEKLREVEQYWTKTKRIEMSMEALIA
                     LHAFGSEEARHFAEAWRAATEANDLDTMQRLVRQFGELMRTELQEG"
     gene            complement(9557..10312)
                     /locus_tag="IQ64_RS06135"
                     /old_locus_tag="IQ64_06450"
     CDS             complement(9557..10312)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987974.1"
                     /locus_tag="IQ64_RS06135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_06450"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_046912948.1"
                     /transl_table=11
                     /translation="MSEQPPYLRIADELRRRIAEHVWEPGDRLPSRAQIGQECGVGENV
                     VRRAQELLISQGVLEGRAGSGTYVAEPRHRVRVVRSSAREQSDGSPFRVDMRAAGSQGD
                     WESRTDAKVPAPAEIAARLGIAEGDLCVRTAYEFLANGRPVQLSTSWEPYDLTAGSLVV
                     LPEGGPHAGAGVVNRMAAIGITVSHAVEQPEPRLATAEEASLLRVQKAALVTHIRRTYY
                     SDQGRPVETADIVVPAAHCEIVYEIPINR"
     misc_feature    complement(10314..10328)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.6"
                     /tool="antismash"
     misc_feature    complement(10352..10365)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.93"
                     /tool="antismash"
ORIGIN
        1 cgccaccatc agcgtccagc cgccccacgc gccggccgcc gaacggccca tggccacatt
       61 gacctgcgcc accgtgtaga gccccgaccc gctggagcgc accagctccg tgacgtccgc
      121 cgaggcctgg aaggcgtccg cgccccgcgt cacccggtgc cccaccaccg agtcggcgag
      181 cacctccttg tacgcgccgc cgggctccgc gatcagcacc cgcccgttgt ccttcggcgg
      241 cttctgttct cccacccgca ggttcccgcc ccagtacagc cgcgcgtacg acacccgcgc
      301 gcccggcggc agccggacct cgccccggct ggagttgtag gtgttcgggt cccggtcgac
      361 gtcgatgtag aacatgtcga agtcgccgtt gacggccgta cgccccgcac gcgcgtcggc
      421 gcacgaggac accgcgggag cgaccgcggg agcgggcgtg agtgccgcgg gcgcggccgg
      481 tgtcgtggag ggtgcggccg cgggcgaggc ggccggcacc gccgcacggc aggtgatcga
      541 ggagttggcc gcgcggacga tcccgccgtg ctgcagcgcc cggtaacgct gtccgaacgc
      601 caggcgctcg gcctcggggg agggggcggg tggcgcggcc gtcgcgggag cgccgggccc
      661 ccagagggcg gcgagggcgc agcagcccac catcgcgcgg cgcagcagag gacccgggtg
      721 aattcgcatg accggcggtg ccctttcggg agctcgttcg ggtgcttgtc ggggaaaggc
      781 cgcggaccct cgacgggcag aaaatgtgcg gatctgataa aggccgcggt gttgtgagga
      841 gatgcgcact ctatcccgct atgcgtactt atctacggaa cgcgccaact gtgtcggaac
      901 ggtgaagcgg gcgttctgcg agatcactcc tttggaggca caaccccgga cgcatgccac
      961 gcgttgactc accgccgggc acccccgccc gagtgttttt tgcatcaact ccaaggagca
     1021 cctctccatg tcgcgtatcg cgaagggcct ggccctgacc tccgttgctg ccgcggccgt
     1081 ggcgggcacc gccggcgtcg ccaccgccga cagcgaggcg aagggcttcg ccgcccactc
     1141 cccgggcgtg ctgtcgggca acgtcgtgca ggttccggtc cacatcccgg tcaacgtgtg
     1201 cggtaacacc atcaacgtca tcggtctgct gaacccgtcg ttcggcaaca agtgcttcaa
     1261 cgactgacgt tcgaggactg acgtccccgc actgacgtct ccggactgac gttcgcacgt
     1321 tccacacgaa ccggccgttc tccccacggg gagggcggcc ggttcgtgtt gctccggatg
     1381 ggccccatcg gttgcagtcg gcgatcagcg atttcacggt gaaaacacga tccctcgcac
     1441 cgatgaaagg aacatccatg cgtgttctgc ccgcacggcg tctcgcggtc tccgcgctct
     1501 gcgccacgct cctcctcggc gtcaccgccc cggccgccct ggccgccgac gtcgagtcgg
     1561 cacgcgagcg cgcccggtcg gcggcccccg tccccggcgc ggacgcgctc caggaccagc
     1621 tcaagggtct cggcgacctc ggcggggtgc tgaagccggt gaccgatctg ctcgacgccg
     1681 tactcgaggc ggacaacggc cagctctccg ccgaccgggc ggccgagctg ggcaaggcgg
     1741 tgcgggacgc catcgaccgg gccaaggcgg gtgcgccggt gacctcgccg gccgttcctc
     1801 cggcggttcc gccggtcgcc ctgccggtgg ccccgcccgc gcccgctgtt ccgcccgcgc
     1861 ctgccgttcc gcccgcggcg acgacgccca cggtcccgtc cgtcccggcg gctccttcgc
     1921 tcccgtcggt gcccaccgcc ccggcggccc ccgtggtgcc ggccgctccg cagcaggcgg
     1981 tcacgctgcc ggcgttcgac cgcgctctcg tgcgcccggc cggcagcgtc gcggcgaacc
     2041 ccgtgaccga cgcgctcacc gccctgcaga aggcggtcga caccctgctc aaggcggtca
     2101 cctccggtga cctcgcccag gtgctgcccg ccgtcaacag tgtggtcgcc ggtctggtcc
     2161 agctcatcgg gtccatcctc ctgggcagcg gcctgcccgc cccggtcgcc ctgccggccg
     2221 tgcagtcccc ggcggagctg ccggtgacgc cctccacgga cacgctgccg gtacggccgc
     2281 cggccgtgcc gcccgtgcgc tgagaattcc gctggcagcg gctctccgca ggccgtcggg
     2341 ccgaccgccg tccgggtcgg tccgacggct gtttcgtatg acctgttgtt cggcgtcaga
     2401 tatgacctgt ccgaaacggg gtttccgtcg gcgcgggggc tcgttggaca cggcgcagtg
     2461 cttccagtat ccgaggagag gaacacgatg aagtcgctga aggccgccgc tgtcgtcgca
     2521 gggtccctgg ccatcgccgg tgccgccgca ccggcttttg cctacgacgc cacggacgtc
     2581 acgcccacca gcctcaacgg cgcggtgaac acgctcttca aggggcccat cgcgcccgct
     2641 ccgatgcagc agcagtcgaa cgcgctcgac acggagaaca aggactccgt cctccacacg
     2701 gtgagcggcg cgaccaaggc cctcaacgcc cccggcggcc ccagcaagct cctgggcggt
     2761 ctgcccctcc agggctgaca tttcctttcg cagtgtgtgc ccagggccgg ttccgcggaa
     2821 aaagcggaac cggcccgacg cgctttccgc tcctccgtcg agtgaattcc ccggtgttcg
     2881 aggcgtgtcc ctcgttcgtg tggtcggtcg tcgcctgtcg cccggtcggg tgaaaacacg
     2941 gcccagtcgt ttggcgggcg tggatagggt tccgcggtga cttcaacttc cgctgaaacc
     3001 cgccctttcc gtcccgccga cctcggaacg ctcgtcgtca tggcgtggag cggtgaagcc
     3061 cccgacggag acatgcccta tctgctcgcc tacaccctgg gcgacggccg gaacggaccg
     3121 gagggctcca cggccgccgt ggcggatctg ctgaccggtc tcggcctgtc catcggcgag
     3181 aaggtcgtcg acggcgccgc ccaccccagc ctgccggtca ccctgctcgt cgaggccggc
     3241 caggccgtca tcaccctgcc gcagctcaac gcccagtgcc cggccccgcc ggagtggctc
     3301 gccgccgtcg gcacccgcgg cttcgcctac ctgctgttcg ccacccgccc ctggcccgag
     3361 gcgcggcccg gcatgcccgt cgagcccgcg gccctcgccg ccttcgcggg tgaccccgag
     3421 acactgacca gcgccgccca cgtcctcctg ccggcccgca gcctgcgcgg ctgatggccg
     3481 tcccggcagg gcggccggcg cgcacggccg gcggcggccg tgccttcgcg atcctgctgc
     3541 tgctcaccgg cgcggccggt ctgctcgcgt cctgggtcat cacgctcgac aagttcaagc
     3601 tgctggagga cccgaacttc acccccgggt gcagcctcaa cccggtggtc tcctgcggca
     3661 acatcatgaa gagcgaccag gcctccgcct tcgggttccc gaacccgatg ctcggactgg
     3721 tggcctacgg ggtcgtcgtc tgcgtcggcg tgagtctgct ggccggagcg gtgttcccac
     3781 gctggtactg gctgaccttc ggcgcggggg cgctgttcgg ggtcggcttc gtgacctggc
     3841 tgcagttcga gtcgctgtac cggatcaact cgctctgtct gtggtgctgt ctggcgtgga
     3901 tcgccacgat cgttctcttc tggtacctgc tctccttcga cgtccgcaac ggtttcctgc
     3961 ccgcgccggc ctggctgagc ggctttctcg tcgagttcac ctgggtgctc ccggtgctgc
     4021 acctcggagt gatcgggatg ctcgtcctga cccgatggtg ggacttctgg acaagctgac
     4081 ctgcggatcg acaggtgagt ggtgcaatgg gccgatgggc ggcaggccga cggtcaagag
     4141 accgatgagg caataggaca tcgaggcaat aggacattga ggcaatgggt cgatgcggca
     4201 atgggcccat aggcctctcg gaccattgcg gcagtcgggc gaatcttgaa ctgagggaga
     4261 tccccggatc gttcacccgg tacgggcgcc gagccccgca gcacggctcg cgaaccgccg
     4321 attccgtatc cgccgatccg gccgattccc acggtcgccg atcgcggatc cgcccatgct
     4381 cgaaagggac cgtaccgaga tgaagccgac cacacgagga acccttgccg ccgtcgtcac
     4441 cggtgtggcg gccgcgatgg gcgcctccgc cgcccccgcc gtcgcggccg agacggtccc
     4501 cgtccccctg ccgctcgaag gggtggagca ctccctgaac gtgaaggcgc ccaagctgag
     4561 cggagagctg ccgctgctca tgccgggcgc tccccaggga ggaccgaagt acgtcgaggg
     4621 acggctgctg cccgcgagca gcctgcccca ggtgccgctc ggcaccgcgc tgccggggct
     4681 gaaggccgag accccgctgc cccgggtcgt cggggagggc ttcgagcggc tcggcgtcga
     4741 cgcccccgcc gccgacctgc ggaccctgac ccccgggctg aacgtggaca ccccactgac
     4801 cgcgcccgac ccccacgcgc tgggcctgcc cagcctgaag gacccccagg tggccgtcct
     4861 caccccgctg ctgcggacgg cgccgaccgc gaacctcggg ctcgtctgag cccgcggcga
     4921 cacggagacg aggcccgggg ccctgacagc ccgggcccgg ccgtccgggg gtcaccgcta
     4981 ccgaacacga cgaggagaga gtgttgttcg tcagcagagt gacggtggac cccgaagccg
     5041 gccggagcag aacgcgccgc aacgcgctgc gcgtcctggg cgcgtcggcc gtcgccctgg
     5101 ccgtggcccc cgtcgtcgcc gccgcgtggc cccgccggtc ggcgcggccc ggggaggtcg
     5161 acttcgacga gacgttccgg ggccgccgta tccggggggt cagggccgac acgggccaga
     5221 cggcctatct cggggcccgc acgcccggcc ccggcgagtg ggacgtcacc gtcgacgggc
     5281 gcccgcttca tctcatgcgc cgcgcggacg gcacgtacct gagcatgatc gaccactacc
     5341 gctcgtaccc gacggccctg gaggcgaccc gcgccgccgt cgaggaactg gggaccacgc
     5401 agcgcctgcg cgtgggggag acgtcggagg gagcggtcgg ccatggtcta cacgcgtaag
     5461 gacgtcagca cgctcacgcg cacggagcgg cggcggttcg tgggcgcgct gctgaagctc
     5521 aaacagcgcg gcgagtacga cgaattcgtg cggatgcaca tcgaccacta cgtctccgac
     5581 ggcgacggcg gtctgcgggc ggcccacatg gctccctcct tcctgccctg gcaccgccgc
     5641 ttcgtgctgg agctggagaa ggcgctgcgc cggatcgacg actcggtgac cgtgccgtac
     5701 tgggactgga cgcgggaccg tacgacgacg tcggtgccct ggacggccga tctgctcggc
     5761 ggcaacgggc ggcgctccga cctgcgggtg acgaccggcc cgttcgcgta cgggacgggc
     5821 cggtggacca tcacggaagg gctcacggac atcgagtacc tcacccggga cttcggctac
     5881 ccgcgcaacc ccgtcggcct gcccaccgcc cgggacctgg acgcggcgct cgccgagtcc
     5941 gtctacgaca aggctccgtg gaactcggtc acccgcgggg gtttccgcaa ccggctcgaa
     6001 gggtggggcc agggcaccgg caacggctcc tggctcaacc acaatcgggt gcaccgatgg
     6061 gtcggcgggc acatggtcgg cggcgcctcc gtcaacgacc ccgtcttctg gctgcaccac
     6121 gccttcatcg acctgcagtg gacgcgctgg cagcggcgtc atcggggcgc gcgctacctg
     6181 ccggccaagc cgccgggcaa gggtgactcc cagcgggggc ggatcgtcgc gcgcgacgag
     6241 cggatgccgc cctggaacgt gaccccgggc gcgctggagt cgatcgggca cgtgtaccgg
     6301 tacgggtgac cggccgcggt ctccgggttt ccgcgcggcg tgaagccccc gacggtggga
     6361 caccgtcggg ggcttcttgt cactgccgtg tcgctgctgt gacgctcggg gctgtgggcc
     6421 ccgtcagtgg ccgtagccgg acgggccggg gttgcccttg tggttgtcgt ggctcttgtt
     6481 gacacagacg ttgccgaagg ccgggttcag gaggccgacg acgttgatcg tgttcccgca
     6541 caggttgacc ggcacgttga cggggacctg gatgatgttg cccgacagga cacccggcga
     6601 gccgatggcc ttggcctcgg cgccggcgtc cgccgcggcc aggccggcgc cgctgagcac
     6661 gacggcggac gtgccgaggg tgacgacggc tgccttcgcg atgcgagaca tcacgttctc
     6721 cttcttgatc ggtgagcacg acccccactt cgtggccgca cgccccttca acgcgcccgc
     6781 gcaccgcggg tcacggcgtc cggcggggga tcacccgttc cgaacgggag tatggggcgg
     6841 ggcgggcggc cggtcgtggg ccaatcgggg gggccggtcg ggcggccggg cgtgggccaa
     6901 tcggggggcc ggtcgggcgg ccgggcgtgc ggtcggccgg tcgagcggcc gttcgtgcgg
     6961 agatccggcc gaaggtgctc aggaccgggt gtcgttctcg gtgatttcgg cgatcagggc
     7021 ctcgacgcgg gtcctgatcc cgtcgcggat ggggcggacc gcctcgacgc cctgtccggc
     7081 cgggtcctcc agtgcccagt cgaggtactt cttgccgggg aagaccgggc aggcgtcgcc
     7141 gcagcccatg gtgatgacgt agtcggaggc ctggacggcc tcggtggtca gcaccttggg
     7201 cgcggcgccg gcgatgtcga cgccgacctc cttcatggcc tccaccgcgg ccgggttgac
     7261 ccggtcggcg gggagggagc cggccgagcg gacctcgatc cggtcgcccg cgaggtgcgt
     7321 caggaaaccc gcggccatct gggaacggcc cgcgttgtgg acgcagacga agagcacgga
     7381 ggcgaggggg gaggtcatgg cggggtcgct tcctgtgatg gctggttctg gttctggttc
     7441 tggttctgct tctgtttgtg gttgcgggtc ggctcgcatc agcctgaggt gatgtgacaa
     7501 tatcagcgca tgctgacgtc agtcgatgct gaatcaacgc agggcccgga ccgggacccc
     7561 gaccaggatc cggaccggga cgcgctgcgg gacctgatcc gggttctggc cgatccgctg
     7621 cgcctcagga tcgtcaccct gctcgcgcgc gagaccctct gcaccacgca tctggtggag
     7681 gagacgggcg ccagacagac caatctctcc aaccacctga aggtgctgcg cgaggccggg
     7741 atcgtggaga cggaaccgtg cgggcggtgg acgtactacc ggctccggcc ggaggccctc
     7801 gcctccctcg cgggcggctt cgccgaactg gcggaggccg cgcgggtgac tgtcgagaac
     7861 gacgtgaagc ggtcctgttc ctgaaccggc gtcgccgggt gatgtcgagg agtggtgttg
     7921 gctgccatcg aggagagtgt cgcgggggcc ggggccgggg ccggggccgg ggccggggcc
     7981 ggggccggtg ggccggtcgt cgacatcggc atcgacacgg tcgccgtcgc gcccgggcgt
     8041 accccgttgg gggcgcgcgt cgccgccgag ttcgtcggta cggcggcact ggtcaccgtg
     8101 gtcgtcgggt ccggcatcca ggccaccgcg ctgacgcctg acgtcggggt gcggttgctg
     8161 gcgaattccc tggccaccgt cttcgggctc ggggtgctca tcgccctgtt cgggccggtc
     8221 tccggggcgc acttcaatcc cgtcgtgacc tcggccgagt ggtggacggg gcggcggcgc
     8281 ggcggtgtgc ggggccggga ggtcgcggag gcggcgggcg ccacgggcct cacggctcgg
     8341 gaggcggtcg cctacgtcgc cgcgcagacc ctcggggcga tcgccggagc cgtactcgcg
     8401 gacgcgatgt tcgcggagcc cctggtgaag tggtccacgc acgagcgttc ggcggggcac
     8461 ctgctgctgg ccgaggtggt cgcgacggcg ggcctggtgc tgctcgtcct cggcctcgcc
     8521 cgttccgacc ggctgcggtt cgcgccggtc gcggtggcgt cgtacatcgg ttcggcgtac
     8581 tggttcacct cgtccacgtc cttcgccaac ccggcggtga cggtggggcg cgccttcacc
     8641 gacaccttcg cgggtatcgc gccggggtcg gtcgccgggt tcgtgggggc gcaactcgtg
     8701 ggtggggcca tcgggttggg actggtagcc gtcatctggc catcttcgag ccgcctcgcg
     8761 gagcgcgtgc ggcgcgccgc cgccccgtcg gtcgccgacc ggcgggtgtg acccggcccc
     8821 ggaggggcat gtggtccgag ccgccgtggc agaaaaaagc cctcccggga ggagggtgag
     8881 gtgtgggcgc ccccggcagg actcgaacct gcggccaagc gcttagaagg cgcctggcta
     8941 gtaggagcct cgacaacctc tgagctgggc aattgcgttg ctcgaccagc tagaaggacg
     9001 cccttcgaca cgcactcgac aggccgaggc tagatcgctt tcgctggggc acctgtggct
     9061 agccttcctg gagctcagtc cgcatgagtt ccccgaactg cctgacaagc cgctgcatcg
     9121 tgtcgaggtc gttggcctct gtggccgccc gccatgcctc agcgaagtgg cgagcctcct
     9181 cgctgccgaa cgcgtgcagg gcgatgaggg cttccatgct catttcgatg cgtttggtct
     9241 tcgtccaata ctgctcgact tctcggagct tctcgatctt tccggaccgt tcgtacctca
     9301 ggcggcgcac atcgaggacg gccacgcgaa gggcttcgaa gtaggcatcg cgccgctccc
     9361 ttcgtaggta agtgtgctgc tcggcttctc gtgactgcgt aaccatgcgc tctgcgtgtg
     9421 ttctaccttg cgctccggcg agccatgtga aggtgacgcc gagggctcct gagactccgg
     9481 ttaccacagc ggggatccac tccgatgcca tgtattcatc ttgcccagat gagtcagcac
     9541 ccaaggcgat ctagagctag cggttgatcg ggatctcgta gacgatctcg cagtgcgcgg
     9601 cgggcaccac gatgtccgcg gtttccacgg gccgcccctg gtcgctgtag tacgtccgcc
     9661 ggatgtgcgt gacgagcgct gccttctgga cgcggagaag cgacgcttcc tcggcggtcg
     9721 ccagccgtgg ctcaggctgc tccacggcgt ggctgacggt gataccgatc gcggccatgc
     9781 ggttcacgac acccgccccg gcgtgtggcc ctccctcagg gaggacgacg agtgagccag
     9841 ccgtgaggtc gtacggctcc caactcgtcg agagttgcac gggcctgccg ttggcaagaa
     9901 actcgtacgc cgttcggacg cacaggtcgc cttcggcgat tccgagacgc gccgcaatct
     9961 ccgccggtgc cggcaccttg gcatcggtcc ggctctccca atccccttgg ctgccggcag
    10021 ccctcatatc cacgcggaac ggggacccgt cggactgctc tcgcgccgac gagcggacga
    10081 ctcgcacccg gtgcctgggc tcggcgacgt acgtgccgga cccggcccga ccctccaaca
    10141 cgccctggga gatcagcaac tcctgcgctc ggcgcaccac gttctcgccc acgccgcact
    10201 cctggccgat ctgggcgcgg gagggcaggc gatcccccgg ctcccagacg tgctccgcga
    10261 tgcgccgccg aagttcgtcg gcgatgcgga gatagggcgg ctgctcagac atatggaaaa
    10321 tctagtccac ttctaatcta gttaactaac ttcactcaaa gtgatcgccg gtgacggagg
    10381 ctgccctgtg cccgcttcgg gagttagcgc ggcgtccctc gccgcccggc tgtccgccat
    10441 cgggcttctc acgcgagtg
//
