LOCUS       NZ_KQ257845            11347 bp    DNA     linear   CON 02-JAN-2025
DEFINITION  Streptomyces stelliscabiei strain P3825 scf_652_13.1, whole genome
            shotgun sequence.
ACCESSION   NZ_KQ257845
VERSION     NZ_KQ257845.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces stelliscabiei
  ORGANISM  Streptomyces stelliscabiei
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 186868)
  AUTHORS   Harrison,J., Sapp,M., Thwaites,R. and Studholme,D.J.
  TITLE     Genome sequencing of plant-pathogenic Streptomyces species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 186868)
  AUTHORS   Harrison,J., Sapp,M., Thwaites,R. and Studholme,D.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2014) Biosciences, University of Exeter, Geoffrey
            Pope Building, Stocker Road, Exeter, Devon EX4 4QD, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Coverage                   :: 140.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001189035.1-RS_2025_01_02
            Annotation Date                   :: 01/02/2025 01:49:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,536
            CDSs (total)                      :: 9,455
            Genes (coding)                    :: 8,973
            CDSs (with protein)               :: 8,973
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 482
            CDSs (without protein)            :: 482
            Pseudo Genes (ambiguous residues) :: 0 of 482
            Pseudo Genes (frameshifted)       :: 129 of 482
            Pseudo Genes (incomplete)         :: 407 of 482
            Pseudo Genes (internal stop)      :: 20 of 482
            Pseudo Genes (multiple problems)  :: 69 of 482
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:19:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 43181
            Orig. end                         :: 54528
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KQ257845.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11347
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11347
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11347
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6347](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6347)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(503..516)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.74"
                     /tool="antismash"
     gene            594..1325
                     /gene="hemQ"
                     /locus_tag="IQ64_RS02095"
                     /old_locus_tag="IQ64_02190"
     CDS             594..1325
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133767.1"
                     /locus_tag="IQ64_RS02095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_02190"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_005481890.1"
                     /transl_table=11
                     /translation="MSDVAPSTEPGRIPNKGKLAKDLNEVIRYTLWSVFQLKDVLPEDR
                     TGYADEVQDLFDQLAAKDVTVRGTYDVSGLRADADLMIWWHAETSDQLQEAYNLFRRTR
                     LGRALTPVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWMLAFEADELYRIVDLMRHLRASEARMHVR
                     EEVPFYTGRRKSVAELVAGLA"
     gene            complement(1378..2961)
                     /locus_tag="IQ64_RS02100"
                     /old_locus_tag="IQ64_02195"
     CDS             complement(1378..2961)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (99-300). E-value:
                     1.4e-19. Score: 62.6. Matches aSDomain:
                     nrpspksdomains_IQ64_RS02100_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481892.1"
                     /locus_tag="IQ64_RS02100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_02195"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_050398063.1"
                     /sec_met_domain="PF00561 (E-value: 8.9e-27, bitscore: 87.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAVLHVTAAAVLLLSGLAAPPAAGDTLTGRVAERAAAEGIRFG
                     KCPAVEGLPDGVRCGTVEVPLDYARPDGRQIALTVSRIRATGKDAEGKKVARQGALVFN
                     PGGPGASGMFFPLLGHLPEWKRLGAAYDLVGYAPRGVGRSAPLSCRDPERLHHGPSPAP
                     THPSEAYKKERIARAEEFARECARRGGSALRHYHSLNNARDLDVLRAALREPRLTFMGA
                     SYGTYFGALYASLFPSHVRRMVFDSAVNPDPEQIWYRNNLGQSAAFEGRWADFRSWAAR
                     HHTVYGLGDTPEKVLRSYERAAALLAARPAGGKVGPGQLQGAFLQAGYHDDLWPQRALA
                     LSAYLKGDPKPLVQIAGPYPEAAVEQENTSAVYTAVECNDGPWPTDWRVWDRDNTRLAR
                     TAPFETWDNVWTNLPCAYWTGPRQQPLDVRTGPGELPPTLILAAERDAATPYDGALELH
                     RRLWGSVLVTERDAGNHGVAWGTNKCVNGHLEAYLLEGRLPERHAACAARPEPSASRGR
                     AAGTPRAVKS"
     aSDomain        complement(2062..2664)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IQ64_RS02100_Abhydrolase_1.1"
                     /evalue="1.40E-19"
                     /label="IQ64_RS02100_Abhydrolase_1.1"
                     /locus_tag="IQ64_RS02100"
                     /protein_end="300"
                     /protein_start="99"
                     /score="62.6"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASGMFFPLLGHLPEWKRLGAAYDLVGYAPRGVGRS
                     APLSCRDPERLHHGPSPAPTHPSEAYKKERIARAEEFARECARRGGSALRHYHSLNNAR
                     DLDVLRAALREPRLTFMGASYGTYFGALYASLFPSHVRRMVFDSAVNPDPEQIWYRNNL
                     GQSAAFEGRWADFRSWAARHHTVYGLGDTPEKVLRSYE"
     gene            complement(3067..3834)
                     /locus_tag="IQ64_RS02105"
                     /old_locus_tag="IQ64_02200"
     CDS             complement(3067..3834)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481893.1"
                     /locus_tag="IQ64_RS02105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_02200"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_050398064.1"
                     /transl_table=11
                     /translation="MLWVLFLLLAWAAAGVSCARLCLASLRAAAAADDVPADRVHHCHR
                     LTLYEAAFLSGGPARVADLTLVSMACRRRILLAHTGWATVVDPDGRDDMERAVIGAIGP
                     GGQSRIAPVRRRAAAAESVRHLSDRLVAAGLAVPDAARTGVADAVTQVRGAALGVCGLG
                     ALASTLPAQGFAEHRALVALWFALPLVLTLSCLLIARVEGPSGTRWASPEGMRVLKAVV
                     GRGGSDVPDLVSVAVRGVGAVESPELRAAFEQR"
     misc_feature    complement(3091..3093)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3977..4750)
                     /locus_tag="IQ64_RS02110"
                     /old_locus_tag="IQ64_02205"
     CDS             complement(3977..4750)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481894.1"
                     /locus_tag="IQ64_RS02110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_02205"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_050398065.1"
                     /transl_table=11
                     /translation="MRSTRGRGGLFSGTGLIVAGLTATLIALVFPVWSYADRSGTDVSV
                     LNATTMTTRYGPLSALDRDFITKVRLAGLWELPAGQQAETKGTTAGVRTAGEHLIEGHT
                     FLDARVRDVAARLNLPLPNQPNDQQKAWLTTLNAARGETYDREFANILRLAHGRVFSVV
                     AQVRATTRNSLVRALADDANTTVLDHITVLEATGLVDYDAIARDTVSASTPPLTSSPAP
                     PGSPDDPGSPIPVTPSPSPSVYTLPPAANGPSDDG"
     misc_feature    complement(4871..4886)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.83"
                     /tool="antismash"
     gene            complement(5001..6347)
                     /locus_tag="IQ64_RS02115"
                     /old_locus_tag="IQ64_02210"
     CDS             complement(5001..6347)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317893.1"
                     /locus_tag="IQ64_RS02115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_02210"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_046920307.1"
                     /sec_met_domain="DUF692 (E-value: 2.5e-98, bitscore: 321.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQIAQLGTGIGWRPEIADAVERMAGRAGGVDWVEVVAENVCPGH
                     VHESLLRLRERGVTVVPHGVSLGLGGADRPDEQRLRSLAERVEALGAPLVTEHIAFVRA
                     GGALTASPRLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALINWPGEDM
                     TEGQFLYDLVDRTGVRLLIDVANLHTNHVNRGEDPAKALDELPVEAIAYVHVAGGFERD
                     GVWHDSHAHPVPRQVLDVLADLASRVTAPGVLLERDDNFPEPGELERELESIRGVLEKA
                     EVRGPATTVPAAGTVPAAEAEPVAEPEAEPVARQRLGLAQAALLSALVAGTPVPEGFDR
                     VRLGVQARSLAAKRADVVAKVAPELPEILGPSYRPTFVAYAQGHPMTDGYRRDALSFAE
                     HVLLTGRPGDADVQREVRRWWLERSGPAPLSRRPTARLARATRRVLLRR"
     gene            complement(6497..7303)
                     /locus_tag="IQ64_RS02120"
                     /old_locus_tag="IQ64_02215"
     CDS             complement(6497..7303)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481896.1"
                     /locus_tag="IQ64_RS02120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_02215"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_046920305.1"
                     /transl_table=11
                     /translation="MRRSKGSVLVALAIAGTLTAVAYPIFYSYPNRNATAAAALTGDTV
                     TTQWGPLTPGDRDLLIRVRLAGLWELPAGQQALERSGSKAIREAADHLIVGHTDLDKRS
                     RVIAAKLGVELPNQPTAEQQGFLDQMTAAKTEADYNKVWANLLRTAHGKIFPAIASVRN
                     STRNTLIRQLASDANQTVLDHITILEATGEVDFATIAQKSVTGTASPTGPPPPVAGQVP
                     PAAPPAEPSTNPDVKSRPSPFPGNQVPTGRPDPEEINKDQGEPAVQ"
     misc_feature    complement(7261..7281)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.42"
                     /tool="antismash"
     gene            complement(7424..8032)
                     /locus_tag="IQ64_RS02125"
                     /old_locus_tag="IQ64_02220"
     CDS             complement(7424..8032)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481898.1"
                     /locus_tag="IQ64_RS02125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_02220"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_050398066.1"
                     /transl_table=11
                     /translation="MRRLNGSLIAGLALVVTVGALAFPVWSYADRSGTAQANMAAGSVN
                     TQWGPLTASDRDLIVRVRLAGLWELPAAEKAMARSKSPQVKEAADHLIVGHKDLDERVR
                     AVASQLGVELPNVPNEQQQGFLQQMDNATDDQFDRVWANLLRSAHGKIFPAIGTIRNQT
                     ENTLVRQLASDTNQTVLDHITMLEKTGQVDFDAIANGTI"
     gene            complement(8378..9118)
                     /locus_tag="IQ64_RS02130"
                     /old_locus_tag="IQ64_02225"
     CDS             complement(8378..9118)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999910.1"
                     /locus_tag="IQ64_RS02130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_02225"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_046919965.1"
                     /transl_table=11
                     /translation="MTTSDPTPDPAPAAAQDSPFQEERTSRDEGPQFVLPLVARIERGA
                     PPARTDALETAARAVLVMLSDARSLGDGEWAEAMRSWQDARIRKVVRRARGAEWRRAEA
                     LPGITVAGKSAEVRVFPPVPLDGWPKDLARLQVSGTDLDDPEPPTEPDPAAPVLWLNPG
                     VEMSAGKAMAQAGHGAQLAWWDLDAPARATWRDADFPLAVRTTTPVHWKTLTTSGLPLV
                     RDAGFTEVAPGSSTVVADHRALRT"
     gene            9189..10148
                     /locus_tag="IQ64_RS50225"
                     /old_locus_tag="IQ64_02230"
     CDS             9189..10148
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999909.1"
                     /locus_tag="IQ64_RS50225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_02230"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_046919966.1"
                     /transl_table=11
                     /translation="MERELLLRLEGVGRRYGFRGPWVLRGVDLEIAPGTLTRVEGANGT
                     GKSTLLRVLAGLDAPTVGRATGRPRTAYVPERFPAALPFTAAEYLTHLGAVHGLDRPAA
                     ARAATEWLERFGAAEYARTPMGELSKGSSQKVAVAQALLAAPELLVLDEAWTGLDADAR
                     EELERVVVERTAAGAAVVFVDHDPGRLAGAPDAIYAVVGGALDHREGSGTSRMGADDAS
                     AASAAAPAVVVEGRGASGRRVPRQITELATAVEESGSGTHRFTVPAAASDALLRALLEA
                     RPPWHVVSLGPAPAPLTAPDSAPESDSAPQPALEGERA"
     misc_feature    9201..9203
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            10148..10819
                     /locus_tag="IQ64_RS50230"
                     /old_locus_tag="IQ64_02235"
     CDS             10148..10819
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481903.1"
                     /locus_tag="IQ64_RS50230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_02235"
                     /product="hypothetical protein"
                     /protein_id="WP_046919967.1"
                     /transl_table=11
                     /translation="MNALLRYQAALLVRSQRWLPPVILYAAFLAIGVQKGQPVLDSLGY
                     TAAALLPVSAWLMRICVTNEPPAARSCTGAAAGPGRAHLACVLVAFSAAALLGTAATVL
                     VAIISAPTSTDHQTRVEALPAAGAGLLAALACALLGTAVGALTNWPLLRSPGRSVPTLL
                     LGALLSLVVAGSPARIAVTALVDGSRRAAVPVPVLPLIAAGLLAAAATAVACALTTRRS
                     A"
     gene            complement(10811..11002)
                     /locus_tag="IQ64_RS50235"
                     /old_locus_tag="IQ64_02240"
     CDS             complement(10811..11002)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481904.1"
                     /locus_tag="IQ64_RS50235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IQ64_02240"
                     /product="hypothetical protein"
                     /protein_id="WP_046919968.1"
                     /transl_table=11
                     /translation="MSDYRGAEETTAAQGSAPATDEAEPFAECVLCRKPTEYPESHKGV
                     TLCPVCEWREAERTACSG"
ORIGIN
        1 cgccgaggcc cccgaggccg ccgccgaact ggcgggcgtg gagtacgcct cgatggccct
       61 ggtcaccctc gcctaccacc ggtccggcct cggcctcccc gcgggcagcg gcttcctggt
      121 gccgccggtc gacggccgca cgatcaaggc gtcgacgttc tcctcccaca agtggggctg
      181 gatcgccgac gagaacccgg acctgctgat cctgcgcacc tcggtcggcc ggtacggcga
      241 gacggcgatc ctggaccacg acgacgccca cctcgtggac gtgtcgagga ccgacctgcg
      301 cgaggccacc ggcctgaccg ccacccccgt cgcgacccgt gtcacccgct gggacgacgg
      361 cctgccccag tacccggtcg gccaccacgc gcgcgtggcc cgcgtccgcg agcgcctcgg
      421 caagctgccc ggcctcgcgg tctgcggcgc ggcgtacgac ggcgtcggca tcccggcctg
      481 cgtcgcgagc gcctccgccg ccgtggacca gatccacggc gacctgcgcg cggtgcggga
      541 cctcaccgcc gacccggtgc agagcctgca cggcggagcg ggagaatagg gccatgagcg
      601 acgtcgcccc ctccaccgag cccggccgga tccccaacaa gggcaagctg gccaaggacc
      661 tcaacgaggt catccgctac accctgtggt ccgtcttcca gctgaaggac gtgctgccgg
      721 aggaccgcac cggttacgcc gacgaggtcc aggacctgtt cgaccagctc gccgcgaagg
      781 acgtgaccgt ccgcggcacg tacgacgtgt cggggctgcg cgccgacgcc gacctcatga
      841 tctggtggca cgccgagacc agcgaccagc tccaggaggc gtacaacctc ttccgccgca
      901 cccggctggg ccgcgcgctc accccggtgt ggtcgaacat ggcgctgcac cgccccgccg
      961 agttcaaccg ctcgcacatc ccggcgttcc tcgccgacga gacgccccgc gactacgtca
     1021 gcgtctaccc cttcgtgcgc tcctacgact ggtacctgct gcccgacgag gaccgccgcc
     1081 gcatgctcgc cgaccacggc aagatggccc gcggctaccc ggacgtccgc gccaacacgg
     1141 tcgcgtcgtt ctcgctgggc gactacgagt ggatgctcgc cttcgaggcc gacgagctgt
     1201 accgcatcgt cgacctcatg cgtcatctgc gtgcctcgga ggcccggatg cacgtccgcg
     1261 aggaggtccc gttctacacg ggccgcagga agtcggtggc cgagctggtg gcgggcctgg
     1321 cgtaggtccc gcgaggagtg cggggccgtc gcggcagcgg ccccgcggcg cgggcggtca
     1381 gctcttgacg gcccgcggtg tcccggcggc ccggccccgc gaggcgctgg gctccggacg
     1441 cgccgcgcag gccgcgtgcc gctccggcag ccgcccctcc agcaggtacg cctccagatg
     1501 gccgttgacg cacttgttgg ttccccaggc caccccgtgg ttaccggcgt cccgctcggt
     1561 caccagcacc gacccccaca ggcgccggtg cagttcgagc gccccgtcgt acggcgtcgc
     1621 cgcgtcccgc tcggccgcga ggatcagcgt gggcggcagc tcaccgggcc cggtccgcac
     1681 atcgagcggc tgctgacgcg gccccgtcca gtacgcgcac ggcaggttcg tccacacgtt
     1741 gtcccaggtc tcgaagggcg cggtgcgcgc gagccgggtg ttgtcgcggt cccagacccg
     1801 ccagtcggtg ggccagggtc cgtcgttgca ctcgacggcc gtgtagacgg cgctcgtgtt
     1861 ctcctgctcg acggcggcct ccgggtacgg accggcgatc tgcaccagcg gcttcgggtc
     1921 gcccttcaga tacgcggaga gggccagggc acgctgcggc cagaggtcgt cgtggtaccc
     1981 ggcctggagg aacgcgccct gcagctgccc cggtcccacc ttgccgccgg cgggccgggc
     2041 cgccagcagc gccgccgccc gctcgtagct ccgcagcacc ttctccgggg tgtcgcccag
     2101 cccgtacacc gtgtggtgcc gtgcggccca ggaccggaag tccgcccagc gcccctcgaa
     2161 cgcggccgac tggccgaggt tgttgcggta ccagatctgc tcggggtccg ggttcaccgc
     2221 cgagtcgaag accatccggc gtacgtggga ggggaacagc gacgcgtaca gcgcgccgaa
     2281 gtacgtgccg tacgacgccc ccatgaacgt cagccgcggc tcgcggagcg cggcgcgcag
     2341 gacgtcgagg tcccgggcgt tgttgaggga gtggtagtgc cgcagcgcgc tcccgccccg
     2401 ccgcgcgcac tcccgcgcga actcctcggc ccgcgcgatc cgttccttct tgtacgcctc
     2461 cgaagggtgt gtcggcgccg gtgagggccc gtggtgcagc cgctcggggt cccggcagga
     2521 cagcggcgcc gaccggccga ccccgcgcgg ggcgtacccg acgaggtcgt acgcggcgcc
     2581 gagccgtttc cattccggga ggtgaccgag gagcgggaag aacatcccgg aggcgccggg
     2641 gccgccgggg ttgaagacga gcgccccctg ccgggcgacc ttcttgccct cggcgtcctt
     2701 gcccgtggcc cggatccggc tgacggtcag cgcgatctgt ctgccgtcgg ggcgcgcgta
     2761 gtcgagcggc acctcgaccg tcccgcagcg caccccgtcc ggcagcccct ccaccgccgg
     2821 acacttcccg aaccggatgc cctcggccgc ggcccgctcc gccacccggc cggtgagcgt
     2881 gtcccccgcg gccgggggag cggcgaggcc gctcagcagc agcaccgccg cggcggtcac
     2941 gtggagaacg gcagcccgca tggcgaatcc ctccgaagca cgacagcgac acagggatgt
     3001 ttggtgcgaa agagggtgaa tgtaaagcac cgtcggacgg tgtcggcgag gtgttcccct
     3061 acggtgtcac cgctgttcga acgccgcccg taactcgggg gactccacgg cccccacccc
     3121 ccggacggcc accgagacga ggtccgggac gtcgctcccg ccccggccca ccacggcctt
     3181 cagcacccgc atcccctccg gcgacgccca ccgcgtgccc gacggcccct cgaccctggc
     3241 gatgagaagg cagctgagcg tcagcaccag cggcagcgcg aaccacagcg ccaccagcgc
     3301 acggtgttcg gcgaagccct gcgcgggcag cgtcgacgcc agcgccccga ggccgcacac
     3361 gccgagtgcc gcgccccgta cctgggtgac cgcgtcggcg acccccgtcc gcgcggcgtc
     3421 cggcacggcc agtcccgccg cgacgagccg gtcggagagg tgccgtacgg actcggccgc
     3481 ggcggctctg cggcgtacgg gcgcgatccg ggactgtccg ccggggccta tcgcgcctat
     3541 gacggcgcgt tccatgtcgt cgcggccgtc cgggtccacg acggtcgccc agccggtgtg
     3601 ggccagcagg atccggcgcc ggcaggccat ggagacgagg gtgaggtcgg cgacgcgcgc
     3661 cgggccgccg gacaggaacg ccgcctcgta gagcgtgagc cggtggcagt ggtggacacg
     3721 gtccgcgggg acgtcgtcgg cggcggccgc cgcccgcagc gaggccaggc acagtcgggc
     3781 acaggacacg ccggcggcgg cccaggccag gaggaggaag aggacccaga gcatggcgtg
     3841 tttctacgcg gcggcccgga cccgcggcgg gcggtgtacg gaatccggag ggaagcttcc
     3901 cggtctcgtt cgttcgtacg ccgttacggc ccgcaattgg aaggccgtcg ggcggcgccc
     3961 gacgcgctcc ggggtgctag ccgtcgtccg acggcccgtt ggcggccggc ggcagggtgt
     4021 agaccgacgg ggacggggag ggcgtcaccg ggatcggcga gccggggtcg tccggtgagc
     4081 cgggcggggc cggggaactg gtgaggggcg gggtgctcgc ggagacggtg tcgcgggcga
     4141 tcgcgtcgta gtcgacgagc ccggtggcct ccaggaccgt gatgtggtcg aggacggtgg
     4201 tgttggcgtc gtcggcgagg gcgcggacca gtgagttgcg ggtggtggcg cggacctggg
     4261 cgaccacgga gaagaccctg ccgtgcgcca ggcgcaggat gttcgcgaac tcgcggtcgt
     4321 aggtctcgcc ccgcgccgcg ttcagggtgg tcagccacgc cttctgctgg tcgttcggct
     4381 ggttgggcag gggcaggttc aggcgcgcgg cgacgtcgcg gacgcgggcg tcgaggaagg
     4441 tgtgcccctc gatgaggtgc tcaccggcgg tccgtacgcc cgcggtggtg cccttcgtct
     4501 cggcctgctg tccggcgggc agctcccaca gtccggcgag tctgaccttc gtgatgaagt
     4561 cccggtccag ggcggacagc gggccgtacc gtgtcgtcat ggtggtcgcg ttgagcacgc
     4621 tgacgtcggt gccggagcgg tccgcgtacg accagaccgg gaagaccagc gcgatgagcg
     4681 tcgccgtcag cccggcgacg atgagtcctg tgccactgaa aagtccccct ctgcccctgg
     4741 tgcttcgcat ggtgcctcct gctcgcggca ccgcgcgctc atgcgcgacg ggtgctgctc
     4801 ggcctacgga ccggtatgtt actgcccggt ccgcgtcaac cgccttatgc ggaggaatgg
     4861 ttgaggtgtg gacaaaaagc tgtggacggg gtaggcgaag cggtggatca accggggcga
     4921 acggggtgca aacgacgcca aacccgtgcg ggaggcacgg gtttgttaat cgcagcacac
     4981 ctcggggtga acgccccggg tcagcggcga aggagcaccc tgcgggtggc tcgggccagc
     5041 cgggccgtgg ggcgccggga cagcggggcg gggcccgagc gctccagcca ccaccggcgc
     5101 acctcgcgct ggacgtccgc gtcgcccggg cggccggtca gcaggacgtg ctcggcgaag
     5161 gacagggcgt cacgccggta gccgtcggtc atcgggtggc cctgcgcgta cgccacgaac
     5221 gtcggccggt acgacgggcc gaggatctcc ggcagctccg gcgccacctt cgccacgacg
     5281 tccgcccgct tggccgcgag ggaccgggcc tgcaccccca gccgcacccg gtcgaacccc
     5341 tcgggcaccg gcgtccccgc gaccagcgcg gacagcagcg cggcctgcgc gagcccgagc
     5401 cgctgccggg cgacgggctc ggcctcgggt tcggcgacgg gctcggcctc tgcggcgggc
     5461 accgttccgg cggcgggcac cgtcgtcgcg gggccgcgca cctcggcctt ctccagcacg
     5521 ccgcggatcg actccagctc ccgctccagc tcgcccggct cggggaagtt gtcgtcgcgc
     5581 tccaggagga cgcccggcgc ggtcacgcgg gaggcgaggt cggcgaggac gtcgaggacc
     5641 tgccggggga cggggtgggc gtggctgtcg tgccagacgc cgtcccgttc gaagccgccc
     5701 gcgacatgga cgtacgcgat ggcctcgacc ggcagctcgt cgagcgcctt cgcggggtcc
     5761 tcgccccggt tgacgtggtt ggtgtgcagg ttcgccacgt cgatgaggag ccggacgccg
     5821 gtgcggtcga cgaggtcgta gaggaactgt ccctcggtca tgtcctcgcc gggccagttg
     5881 atgagggcgg cgatgttctc cagggcgagc ggcacgggca gcgcgtcctg ggcgatgcgg
     5941 acgttctcgc acagcacgtc cagggcgtcc cgggtgcgcg gcaccggcaa caggtgcccg
     6001 gcctccagcc gcggcgacgc ggtgagcgcg ccccccgccc ggacgaacgc gatgtgctcc
     6061 gtgacgaggg gggcgcccag tgcctccacg cgctcggcga gggagcgcag gcgctgctcg
     6121 tcggggcggt cggcgccgcc gagacccagg gagacgccgt gcggcaccac cgtgacgccg
     6181 cgctcgcgca ggcgcagcag cgactcgtgg acgtggccgg gacagacgtt ctcggcgacg
     6241 acctcgaccc agtcgacacc gcccgcccgc ccggccatgc gctcgacggc gtcggcgatc
     6301 tccggccgcc agccgatccc cgtccccaac tgtgcgatct gctccatcgt cccctcctcg
     6361 gtcacgtcgg ccacggcttc accagaccct gtgcgtctcc tcatggcgga cctgtgttcc
     6421 ggcgtgacgg agtcatggcc ccgaggaacg ggggcgaacc ccggggaggg gaccttcaga
     6481 gcaacatttg agctttctac tggaccgccg gctcgccctg gtccttgttg atctcctccg
     6541 ggtccggacg gccggtgggg acctggttgc ccgggaaggg cgacgggcgc gacttcacgt
     6601 ccgggttggt ggacggctcg gcggggggcg ccgcgggcgg cacctgaccg gcgacgggcg
     6661 gcggcgggcc ggtcgggctc gcggtgccgg tgaccgattt ctgggcgatg gtcgcgaagt
     6721 cgacctcgcc ggtggcctcc aggatcgtga tgtggtccag cacggtctgg ttggcgtcgg
     6781 aggccagctg gcggatcagc gtgttgcggg tggagttccg cacggaggcg atcgccggga
     6841 agatcttgcc gtgggcggtc cgcagcaggt tcgcccagac cttgttgtag tcggcctcgg
     6901 tcttcgcggc ggtcatctgg tccaggaagc cctgctgctc cgccgtcggc tggttgggca
     6961 gttcgacgcc cagcttcgcc gcgatgaccc gggaccgctt gtcgaggtcg gtgtggccca
     7021 cgatcaggtg gtcggctgcc tccctgatgg ccttgctccc gctgcgctcc agggcctgct
     7081 gcccggccgg cagctcccac aggcccgcca gccgcacgcg gatgagcagg tcgcggtcgc
     7141 cgggggtgag cggcccccac tgcgtggtca ccgtgtcacc ggtgagcgcg gccgcggccg
     7201 tcgcgttgcg gttggggtac gagtagaaga tgggataggc gaccgcggtg agcgtgccgg
     7261 cgatcgccag ggcgacgagg actgagccct tggagcgtcg caaaagagcc tcccggagga
     7321 aaccaactga tcgttggggt ctgactgttg cttggggtgc ggcgggtgag cgccgggtgt
     7381 gcctggtccg tggccgccgc tcgggggcgg gtccggggcc gcgtcagatc gtgccgttgg
     7441 cgatggcgtc gaagtcgacc tggccggtct tctccagcat cgtgatgtgg tcgagcacgg
     7501 tctggttggt gtccgaggcc agctggcgca ccagggtgtt ctcggtctgg ttccggatcg
     7561 tcccgatggc cgggaagatc ttgccgtggg cggaacgcag caggttggcc cacacccggt
     7621 cgaactggtc gtccgtggcg ttgtccatct gctggaggaa gccctgctgc tgctcgttcg
     7681 gcacgttcgg cagctccacg cccagctggg acgccacggc ccgcacccgt tcgtcgaggt
     7741 ccttgtggcc cacgatcagg tggtccgccg cctccttcac ctgggggctc ttcgaccgcg
     7801 ccatcgcctt ctcggcggcg ggcagctccc acagcccggc gagccgcacg cggacgatca
     7861 ggtcccggtc ggaggccgtc agcggccccc actgggtgtt caccgagccg gcggccatgt
     7921 tggcctgggc ggtgcccgag cggtcggcgt aggaccatac cgggaacgcc agcgcgccta
     7981 cggtgacgac gagagccagg ccggcgatga gcgagccgtt gagacgccgc aaagtgatcc
     8041 tcccggatga tgccaactgg tcgttggggc agttgcttgg ccagtgcagg aagatacgtg
     8101 cggggtggcg gagttgttca acggctttcg aacagcctga gggggtccgg ttcaggcgga
     8161 ctcgatcagc ggggcgcggc gtgggcgccc ggcgccgggg aggtgccgac ggcgaggggc
     8221 cggctgttcg gctgcgggtg ggtgggggct ggtcgcgcag ttccccgcgc ccctgaaaag
     8281 caggggctgc gccccgtgct tttcgtccct gagcggcagg aggccctcaa ggggcgcggc
     8341 ccttgacagg gcggggacgg gtaaggggcc cgggggttca ggtgcgcagg gcccggtggt
     8401 ccgcgacgac cgtgctggag cccggggcga cctccgtgaa gcccgcgtcg cggacgaggg
     8461 gaaggccgga ggtggtgagg gttttccagt ggaccggggt ggtggtgcgg accgcgagag
     8521 ggaagtccgc gtcccgccag gtggcgcggg ccggggcgtc gaggtcccac caggcgagct
     8581 gggcgccgtg accggcctgg gccatcgcct tgcccgccga catctcgacc ccggggttca
     8641 gccagagcac cggggcggcg ggatcgggct cggtcggcgg ttcggggtcg tcgaggtccg
     8701 tgccggagac ctggagccgg gccaggtcct tgggccagcc gtcgagcggc accggcggga
     8761 agacccgcac ctccgccgac ttgccggcca cggtgatccc cggcagcgct tcggcccgcc
     8821 gccactccgc gccccgcgcc cgccggacca ccttgcggat ccgggcgtcc tgccagctcc
     8881 gcatcgcctc ggcccactcc ccgtcgccca gcgaccgcgc gtcgctcagc atcacgagca
     8941 ccgcccgcgc cgccgtctcc agcgcgtccg tacgcgccgg cggagcgccc cgctcgatgc
     9001 gcgcgaccag gggcagcacg aactgcggcc cctcgtcgcg cgaggtccgc tcctcctgga
     9061 aggggctgtc ctgggcggca gcgggggcgg ggtcgggggt cgggtcgctc gtggtcaccc
     9121 gcccagtctg ccaggcgaaa agggccctgc cccgacccgc acccgcccgt ctgtacgagg
     9181 atggggccat ggaacgcgag ttactgctga ggctggaagg tgtcggccgc cgctacggat
     9241 tccgtggccc ctgggtgctg cgcggcgtcg acctggagat cgcgcccggc acgctgaccc
     9301 gggtcgaggg cgcgaacggc accggcaagt cgacgctgct ccgcgtcctc gccggacttg
     9361 acgcgcccac ggtgggccgg gcgacggggc gcccacgcac ggcgtacgtc cccgaacgct
     9421 tccccgccgc gctgccgttc accgccgccg agtacctcac ccacctcggt gccgtccacg
     9481 gcctcgaccg cccggccgcg gcacgcgccg ccaccgagtg gctggagcgc ttcggcgccg
     9541 ccgagtacgc ccgtacgccc atgggggagc tgtccaaggg cagcagccag aaggtcgccg
     9601 tggcccaggc cctgctcgcc gcaccggagt tgctcgtcct cgacgaggcg tggaccggcc
     9661 tggacgccga cgcccgtgag gagctggagc gtgtggtggt cgagcggacc gccgccgggg
     9721 ccgcggtggt gttcgtggac cacgatccgg ggcggctggc gggggcgccg gacgcgatct
     9781 acgcggtggt cgggggcgcg ctcgaccacc gggaggggag cgggacttca cggatgggtg
     9841 cggacgacgc ctcggccgcc tccgccgccg cccctgctgt cgttgtcgag gggcgtggcg
     9901 cgtcggggag gcgagtaccg cgccagatca ccgagttggc gacggcggtc gaggagagcg
     9961 gctccgggac ccaccggttc accgtcccgg cggcggcctc ggacgcgctg ctcagagctt
    10021 tgctggaggc ccgcccgccc tggcacgtgg tgagcctggg cccggcaccc gcgcccctga
    10081 cggcacccga ctccgcaccg gaatccgact ccgcaccgca acccgcactg gaaggcgaac
    10141 gcgcctgatg aacgccctcc tccgctacca agcggccctc ctcgtacgct cccagcgctg
    10201 gctcccgccc gtcatcctgt acgcggcgtt cctcgccatc ggggtgcaga aggggcagcc
    10261 ggtgctcgac tcgctcggct acacggccgc cgcgctgctg cccgtctccg cctggctgat
    10321 gcggatctgc gtcaccaacg agcctcccgc ggcacgcagt tgtacgggcg cggcggccgg
    10381 acccggccgg gcgcacctgg cctgcgtcct ggtggcgttc tcggcggcag ccctcctcgg
    10441 cacggcggcc accgtcctcg tcgcgatcat cagcgccccc acgagcacgg accaccagac
    10501 gcgcgtcgag gcgctcccgg cggcgggcgc cggcctgctg gccgcgctcg cctgcgccct
    10561 cctcggcacg gccgtcggcg cgctcacgaa ctggcccctg ctgcgctcgc cgggccggtc
    10621 cgtgccgacc ctgctgctgg gtgcgctgct gtccctggtc gtggccggct ccccggcccg
    10681 gatcgcggtc accgctctgg tcgacggttc gcggcgggcg gccgtcccgg tccccgtcct
    10741 gcccctgatc gcggccggtc tgctcgcggc ggccgcgacc gccgtggcgt gcgccctgac
    10801 gacccgccgc tcagcctgaa caggcggtcc gctcggcctc ccgccactcg catacagggc
    10861 acagcgtgac ccctttgtgg gactccgggt actcggtggg cttccggcac agcacgcact
    10921 cggcgaaggg ctccgcctcg tccgtggccg gggcggaccc ctgagccgcg gttgtctctt
    10981 cggctccccg gtagtcgctc atccctccag cctagaacgg ccgggccgcg tcccggatca
    11041 gcgcgcgacg gccgccgcgg ccgtctccgt cgtcgccgcc gtgccgacca gttccgacac
    11101 cttcacgaac cggtagcccc gcgcccggag caccggcacg atcctccgta cggccttctc
    11161 cgtgaccggg gcggcattcc gcgtgcagtg catgaccacg accgaccccg gccgcactcc
    11221 gtccagcacc tgccgcacca cgtcgtccgc gtccgtcgcg aacgcgtccc cgctcaccac
    11281 gtcccactgc acggccgtca ccccggacgc gccgatgctg cgcagcgcgc ggctgtcgta
    11341 gcagccg
//
