LOCUS       NZ_CPTT01000022        10285 bp    DNA     linear   CON 13-DEC-2024
DEFINITION  Streptococcus pneumoniae strain 10F/2, whole genome shotgun
            sequence.
ACCESSION   NZ_CPTT01000022
VERSION     NZ_CPTT01000022.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  AUTHORS   Informatics,Pathogen.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2015) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001138385.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 01:30:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,083
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 60 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 32 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:01:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 15846
            Orig. end                         :: 26131
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CPTT01000022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10285
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10285
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10285
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5285](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5285
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     assembly_gap    725..1167
                     /estimated_length=443
                     /gap_type="unknown"
     gene            complement(<1168..1794)
                     /locus_tag="AEH42_RS08665"
     CDS             complement(<1168..1794)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /locus_tag="AEH42_RS08665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAD family hydrolase"
                     /protein_id="WP_050119612.1"
                     /sec_met_domain="HAD_2 (E-value: 1.8e-39, bitscore: 126.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIRG
                     FMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPLYI
                     TTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIIIGDT
                     KFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYF"
     gene            complement(1816..2688)
                     /gene="sdaAA"
                     /locus_tag="AEH42_RS08670"
                     /old_locus_tag="ERS011481_01684"
     CDS             complement(1816..2688)
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /locus_tag="AEH42_RS08670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01684"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="WP_000500038.1"
                     /transl_table=11
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLMER
                     NLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNEHN
                     AKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASISGA
                     EGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEVPCVK
                     RNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLATTPTGR
                     RLQKEIFGE"
     misc_feature    2672..2688
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.79"
                     /tool="antismash"
     gene            complement(2697..3368)
                     /gene="sdaAB"
                     /locus_tag="AEH42_RS08675"
                     /old_locus_tag="ERS011481_01685"
     CDS             complement(2697..3368)
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /locus_tag="AEH42_RS08675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01685"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="WP_000838912.1"
                     /transl_table=11
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPTE
                     VEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKDSN
                     APHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDIPGM
                     IALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHNVNFF
                     K"
     misc_feature    3524..3540
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.23"
                     /tool="antismash"
     gene            3610..4197
                     /locus_tag="AEH42_RS08680"
                     /old_locus_tag="ERS011481_01686"
     CDS             3610..4197
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /locus_tag="AEH42_RS08680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01686"
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="WP_000836442.1"
                     /transl_table=11
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLSE
                     IAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETVSA
                     PVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYS
                     AENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(4250..4570)
                     /locus_tag="AEH42_RS08685"
     CDS             complement(4250..4570)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /locus_tag="AEH42_RS08685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_001861316.1"
                     /transl_table=11
                     /translation="MQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTLL
                     EDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVETHYQPK
                     KL"
     misc_feature    4655..4671
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.88"
                     /tool="antismash"
     misc_feature    4660..4673
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.93"
                     /tool="antismash"
     misc_feature    complement(4661..4674)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.42"
                     /tool="antismash"
     misc_feature    complement(4939..4955)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.57"
                     /tool="antismash"
     gene            5001..5285
                     /locus_tag="AEH42_RS08690"
                     /old_locus_tag="ERS011481_01687"
     CDS             5001..5285
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230167.1"
                     /locus_tag="AEH42_RS08690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01687"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_050119614.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.3e-32,
                     bitscore: 103.2, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFGY
                     SDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASIFKFPPTGMEYFYGF"
     gene            5340..7448
                     /locus_tag="AEH42_RS08695"
                     /old_locus_tag="ERS011481_01688"
     CDS             5340..7448
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /locus_tag="AEH42_RS08695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01688"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_050119616.1"
                     /transl_table=11
                     /translation="MKKISHLCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDLY
                     LKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLDFT
                     TDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEMDKD
                     RIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQPMMEM
                     KRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPMLWLSHF
                     LLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTALLIGVGR
                     SLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFYQDLVEKT
                     QAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTGIKKVILMP
                     ASAKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFPYSDKRKENL
                     SNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKLSDGNYLKFSS
                     IQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRDIITKKFMGWKL
                     VDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVLGLASRMEKRSLV
                     ELLKGGIL"
     gene            7445..8086
                     /locus_tag="AEH42_RS08700"
                     /old_locus_tag="ERS011481_01689"
     CDS             7445..8086
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /locus_tag="AEH42_RS08700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01689"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_050119618.1"
                     /transl_table=11
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLLN
                     LLGLIDGDYSGRYEIFCQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLMLAL
                     KYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARATLKPSQLILADE
                     PTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     misc_feature    complement(8192..8206)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.38"
                     /tool="antismash"
     gene            8292..9098
                     /locus_tag="AEH42_RS08705"
                     /old_locus_tag="ERS011481_01690"
     CDS             8292..9098
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /locus_tag="AEH42_RS08705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01690"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_001038474.1"
                     /transl_table=11
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVALN
                     PDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKADLA
                     ISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQETM
                     AKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNFEKEQ
                     DDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
ORIGIN
        1 tgatcgtttg ttgcaagtct ttgagcagta caaacctgct attgtttatc atgcggcagc
       61 ccacaagcat gttcctatga tggagcgcaa tccaaaagaa gccttcaaaa acaatatccg
      121 tggaacttac aatgttgcta aggctgttga tgaagctaaa gtgtctaaga tggttatgat
      181 ttcgacagat aaggcagtca atccaccaaa tgttatggga gcaaccaagc gcgtggcgga
      241 gttgattgtc actggcttta accaacgtag ccaatcaacc tactgtgcag ttcgttttgg
      301 gaatgttctt ggtagccgtg gtagtgtcat tccagtcttt gaacgtcaga ttgctgaagg
      361 tgggcctgta acggtgacag acttccgcat gacccgttac tttatgacca ttccagaagc
      421 tagccgtctg gttatccatg ctggtgctta tgccaaagat ggggaagtct ttatccttga
      481 tatgggcaaa ccagtcaaga tttatgactt ggccaagaag atggtgcttc taagtggcca
      541 cactgaaagt gaaattccaa tcgttgaagt tggaatccgc ccaggtgaaa aactctacga
      601 agaactcttg gtatcaaccg aactcgttga taatcaagtt atggataaga ttttcgttgg
      661 taaggttaat gtcatgcctt tagaatccat caatcaaaag attggagagt tccgcactct
      721 cagtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa atacgccaaa acttctaagg gtttgtgagc
     1201 gatataatca ggttgatagt ttagtagatc tgcttgctct ccaaatcccc aagtgatggc
     1261 caatttctga atacctgttt ctcgagctcc cagcatatca aacttggtat ctccgatgat
     1321 gatggcttgt tctggtgcta gttgatgtgt ctgcaaggct tggtgaatga catctgcctt
     1381 atggggtgct tcagggctag aaccataaat gccatcaaag aaatgatgga tttccaagtt
     1441 ttttgccatg tcttgagcag tagatgtatc ctttgtcgtg gtgatgtaga gtggataact
     1501 gctcgataac tcctcaagca agtctataat ctgaggaaag agttgagctt catagatgcc
     1561 ttttgcctta tagtaagaac gatatatctg cacggcttca gaaatttggt ctttggacag
     1621 gcaggtcgca aaactacttt cgagaggtgg tcccataaaa ccacgaatag ttttggcatc
     1681 agggctaggc acccccagct ctttaaaggt ataggtaaag gcattgtgaa tcccgataga
     1741 actatcaacg agggttccat ccaaatcgaa aaaaatcgct gtgatagagg tcatggtttc
     1801 tcctatttga taagcttatt ctccgaaaat ttctttttgg aggcgacgac cagtaggggt
     1861 ggtagcgagt ccaccttcag ctgtttcacg aaaggcagtt ggcatgcttg ctcctacttg
     1921 gtacatggca tcgatcactt catccacagg gattttagat tcgatacctg ccaaggccat
     1981 gtctgctgcg atgaaagcaa agctagctcc catggcatta cgtttgacac agggaacttc
     2041 gaccaaacct gcaacagggt cacagatgag gcctagcata tttttaatga caaaggcaat
     2101 agcttgactg gcctgataag gtgttccacc tgcagccaga gtcaaggcgg cagcactcat
     2161 agcagaggct gaaccaactt cagcttgaca cccaccctca gcacctgaga tggaggcatt
     2221 gtttgcgatg actagtccaa aggcaccagc agcaaagagg aaatccaatt gttgctcgtg
     2281 gctgaggtct aatttttcaa tagcagcagt gagaacggat ggcagacagc cagcacttcc
     2341 agcggttgga gtggcacaga ccaagcccat tttggcattg tgttcattga ctgcgatggc
     2401 atttcgggca gccgagagaa tcgtataatc tgacagagtt tttccgtttt cgatgtagtg
     2461 atccaatttg gcagcatctc cacctgtcag gccactacga gatttatttt cattgaggcc
     2521 aagttggaca gaggctttca taacttccag attgcgttcc atgagaagga agacttcttc
     2581 acgttcgcga ccggtcaatt caaactctgt tgtaatcatg agttctgcga catttccttg
     2641 aaagtccaga tctgcttgct cgaccaattc tttgatagaa taaaacatgc ttcctcctat
     2701 ttaaagaaat tgacattgtg gagatgaggg atttttcgaa tttcttcgat agcctcatca
     2761 cagttgcgac tgtcaacttc gataatcata atggcttttt caccagcttt ttcacgagtg
     2821 acattcatct gggcgatatt gataccatag cgggaaagcg cctctgtaac aagggcaatc
     2881 atacctggaa tatcttgatg aacgatgatg atagtcggtg tattcatatt gagagagacg
     2941 gcaaaaccat tgagttcggt tacctgaata tttcctccac cgatagaaat accagtcacg
     3001 ctgatggtct tgtgggcatt tttaacagta attttagtgg tgttagggtg aggggcattg
     3061 ctgtctttct gaatggtcca gacaatcttg ataccacgct tgtgggcaat ttccagacta
     3121 tttggaattt caggatcatc tgtatccatt cctaaaatac ctgcaacaag ggctaggtct
     3181 gttccgtgac cacgataggt cttggcaaat gagttaaaaa gttggaattc aacttctgtc
     3241 ggagtatcat caaaaatgga agagacaatc ttcccaatac gaacagcacc agcggtatgg
     3301 ctactagatg ggccaatcat aactggtccg atgatatcaa agacagattg aaaacgaagt
     3361 gatttcatca gtttcccctt ataaaaattc ttatctctat tatatcaaag aatgaggggc
     3421 ttggctttaa ttgtggatga aaacctttct aatacctcaa atagcataaa aatagtatct
     3481 tttatgacaa aaaacacctt atttagggaa ataaaaaata attttgtaat atttctacat
     3541 aaaagtgtca agaaacggta atatttaaag ggtatgatag aactatagaa agaaggagaa
     3601 ttttcgaata tgaaatcaat aactaaaaag attaaagcaa ctcttgcagg agtagctgcc
     3661 ttgtttgcag tatttgctcc atcatttgta tctgctcaag aatcatcaac ttacactgtt
     3721 aaagaaggtg atacactttc agaaatcgct gaaactcaca acacaacagt tgaaaaattg
     3781 gcagaaaaca accacattga taacattcat ttgatttatg ttgatcaaga gttggttatc
     3841 gatggccctg tagcgcctgt tgcaacacca gcgccagcta cttatgcggc accagccgct
     3901 caagatgaaa ctgtttcagc tccagtagca gaaactccag tagtaagtga aacagttgtt
     3961 tcaactgtaa gcggatctga agcagaagcc aaagaatgga tcgctcaaaa agaatcaggt
     4021 ggtagctata cagctacaaa tggacgttat atcggacgtt accaattaac agattcatac
     4081 ctgaacggtg actactcagc tgaaaaccaa gaacgtgtag cagatgccta cgttgcagga
     4141 cgttacggtt catggactgc tgctaaaaac ttctggctta acaatggctg gtattaagaa
     4201 ctaacgaaca aataatatgt aaagagtcga ggaaatccct cgactttttt tataatttct
     4261 ttggttgata atgggtctct acttcttttt tgagatgaga cagcatagaa gtttcctcgg
     4321 tcagctctag gagtgagaac cctttttgga taagtttcgc atcatcccta caaatcccaa
     4381 tacggacata gcagatagcc agcctatcgt agcttttctt attcttggcg tcctccagta
     4441 aagtgtagca gatttgctta cacatgtttt tatcttgatt gtatagatag agtgtggaaa
     4501 ggtttaatag aattgtcatt cgcaagtcat ataaatgttg atagttttta tagacttcta
     4561 agcgttgcaa gattttctcg gattattgac aaataaacgg acattctgat agacgattgc
     4621 cctagcggct tagtgtaaac tttacaaatt tggcttgtaa tttttttata aaattcactt
     4681 cagttacttg acagttatta tattttcgct tatgcttatt atatagataa aaggacggga
     4741 taggagttta aaaatttgaa agcgctatcc atattgttta ttggactgat tcttgagtaa
     4801 tagttagagt ttgcttaata ctactctggt tgttcaagga cctgatacag aagcgcacca
     4861 ttctcccggt taagagtaag ttcgttgtta aaaaatgaaa tgagatagaa ggaaggtgag
     4921 tccaatggac tacgatactt gttgataaat tactatagct gtttttacgg aacagctctc
     4981 gataatagga gaaataaatc atgagaaaat tagttaaaat tggtgttgca tctttaatgg
     5041 tatgtggtat acttgctact acaaatgctt tagcagtatg ggttgagggt ggtcaatgga
     5101 actatggagt aggttggaca ggaacttttg gatattctga ttacttacat tctactcgat
     5161 atcatacagc aactgttaga catgggggta gaacctctaa ggattatgca aaacctgagg
     5221 catgggctcg ggcttcaatc ttcaaattcc caccaacggg aatggaatat ttctatggat
     5281 tttaattaaa attctccctc cccttttgga gagggagttt cttcacggga ggtatctcta
     5341 tgaaaaaaat cagtcatctt tgtatgttac tcctgcttct gtgtaccact ttttttgttt
     5401 ttaatgtaaa ctatacacga gaagtggttc ggattcaaga aatgggaaag actgtagatt
     5461 ctttggattt gtatttgaaa gatattaacg aacctgcagc gtctgttctt cgattttttg
     5521 aggatgtatc aaaggagtat aaagtctcca tcatcaaaac agacagtggt gatgaggtgg
     5581 tcaagtctgg tgtttttgat aaagatacct tcccctacca agagtttggg atttcttctc
     5641 ttgattttac cacagatggt gaaggagtct atagtaataa agaaatttcc aataaacttg
     5701 gtacgattcc gacctttcta aaagccaaac ctattcagct tatgactttt caaacctata
     5761 tcaaggatac atctcgtagt ttaaatggtc gctatacgat aacttctaca caagagatgg
     5821 acaaggatag gattgtacag aaatggagcg attttttcaa gatagaccag gctaccttgc
     5881 tagagccgac ctacaaaagt gcagtggaag tcataaatcg agatttgctt ttatctgcca
     5941 ttgtttttgt cttggctatt ttgcttcttg tgttagtgac agtgtatcaa ccgatgatgg
     6001 agatgaaaag agttggggta caaaaattac ttggttttca agatagggct gttttagctg
     6061 atgttgtaaa aggcaacctt tacctcctcc taggtggggc tcttgtgatc aatctaggcg
     6121 tgtttttctt gcttgattat aagccaaaag atttgtttcc tatgctgtgg ttgtctcatt
     6181 ttttgctgtt gcagctttat ctctttatca gttggttgac ttacctctta atccaaaaaa
     6241 tgacaatcag ctctctgctg aaaggttttt catctttcaa atttggtctt atcttcaatt
     6301 atgtgatgaa aatagggaca actattttac tgacggcctt actgattggg gtgggcagaa
     6361 gtttagaaca agaaaacaaa gaacttgctt atcagcaaca gtgggtaagt caaggtaatt
     6421 acctgacctt agaaaccttc aaactcaatg ataatctgtg gcaagaagag ctagcagggt
     6481 cagggaaatc tacagattat ttctatcgat tttatcagga tttggtagaa aaaacgcagg
     6541 cgggctatgt gcagagtagc agtcttcctg taaaaaattt tgtccaatca gaacagattc
     6601 agcaatatca gttaacagat acggtggatg tttactatgc caatcgcaat tttctaaaga
     6661 gcaagggatt caagctacca gataccggta ttaaaaaagt tattttgatg ccagccagtg
     6721 ccaaaggtga agaagataaa aatcagctct tggggaagtt aattgccttt cattcgatga
     6781 agtatgaaga gcagcaaaaa cgaacgatag aggagatgga tgtcgagatt gcctattatg
     6841 aaggagattg gtcatttttc ccatatagtg ataagcgaaa ggaaaatctc tccaatccaa
     6901 ttattagctt ggtcaatgat tctgatatga tgtgggatga gaaagcctcc ctgtcaacaa
     6961 ctggcttaaa taatccgatt aaaattgaaa atacggttca acatcaaaaa gagattacag
     7021 agttagttga gaaattgtca gatggaaatt atttaaaatt ttcatctatt caagccattc
     7081 aacaagagaa agtggattct tatcgagatg ctgttcggaa ttttaaccta ctctttgctt
     7141 tgtttggtct ccttagcatg atgatttcct acttcttact agtaacaact ttcttattga
     7201 agcgcaggga tatcattacc aagaagttta tggggtggaa actggtcgat cgctaccgtc
     7261 ctctcctcgt tctgctcttg ctgggctata gtttccctct tctagtcttg attttctttg
     7321 cccatgcgtt cttaccactt ctactgtttg caggttttac atgtctggat atactatttg
     7381 tgctaggctt agcttctagg atggagaaaa gaagtctagt agagttattg aaagggggca
     7441 tcttatgatt gagttgaaaa atattaccaa aaccattggg ggaaaagtga ttttggataa
     7501 cttatctctc aggattgatc agggggattt ggtagctatt gttggtaaga gtggtagtgg
     7561 gaagtcgacc ttgttaaatt tattgggttt gatagatggt gattatagcg gacggtatga
     7621 gattttttgt cagacaaatc tagcggttaa ttctgctaag tcgcaaacaa taatccgtga
     7681 acatatctct tatctgtttc aaaattttgc cctgattgat gatgaaacgg tcgagtacaa
     7741 tctcatgctg gcgctgaaat atgtgaaatt gcctaagaaa gacaagctca aaaaggtgga
     7801 agagatttta gagagagtag gtttgtcagc tactttgcat caaagggtct ccgagttgtc
     7861 tgggggcgaa caacaacgaa ttgcagttgc tagagccacc ttaaaaccca gccagctgat
     7921 tttagccgat gaacctacag gttcgctgga tcctgaaaat agagatttgg tcttgaagtt
     7981 tctcttagag atgaatcgag aagggaaaac agtcattatt gtgacccacg atgcttatgt
     8041 agcccaacaa tgtcatcgtg tcattgaatt gggcgaggga aaatgagttc attcagctcc
     8101 ttttgactgg ctgaatactc atgttttcca gagaaaaata gcataaatac gcctaggaat
     8161 gacattttat gtagtatttc taggtttttt tgtttcaaat tgaaaatttt ttcaatttag
     8221 gcttgacaaa ggatgagtat aggagtatta tttatacaat aaaaaagaat aaacataaag
     8281 gaggctttgt tatgaataag atgaagaagg tgttgatgac gatgtttggt ttagtgatgc
     8341 tccccctact atttgcttgt agtaacaatc aatcggctgg aattgaagcc atcaagtcca
     8401 aaggaaaatt ggttgtagcc ctcaatccag attttgctcc atttgaatac caaaaagtgg
     8461 ttgatgggaa aaatcagatt gtgggttcag atatcgaatt agccaaggct atcgcaacag
     8521 aactaggtgt cgaattggaa ctatctccta tgagttttga caatgtactg gctagcgttc
     8581 aatcaggaaa agctgacctt gccatatcag gtgtttctaa gacagatgaa cggagcaagg
     8641 tgtttgattt ttcaactccc tactatactg caaaaaataa actcattgtc aaaaaatctg
     8701 atttagccac ttatcagtct gtcaacgatt tggcgcagaa aaaggtcgga gcgcagaaag
     8761 gttcgattca agagacgatg gcgaaagatt tgctacaaaa ttcttccctc gtatctctgc
     8821 ctaaaaatgg gaatttaatc acagatttaa aatcagggca agtggatgcc gttatttttg
     8881 aagaacccgt tgccaaggga tttgtggaaa ataatcctga tttagcaatc gcagacctca
     8941 attttgaaaa agagcaagat gattcctatg cggtagccat gaaaaaagat agcaaggaat
     9001 tgaaagaggc agtcgataaa accattcaaa agttgaagga gtctggggaa ttagacaaac
     9061 tcattgagga tgcctttaaa gcgtccattg aaaaatagaa agaaggaaaa gaacatgagt
     9121 aaagaaaaag taattttggc ctattctggc ggtttagaca catcagttgc tattacctgg
     9181 ttaaagaaag actatgatgt tgtttcagtt tgtatggatg tgggtgaagg gaaagactta
     9241 gatttcatcc atgataaggc tcttaaggtt ggggcagtag aatcttatgt cattgatgtt
     9301 aaggacgaat ttgctacaga ttatgtgcta gtggctcatc agtcacatgc ctattatgaa
     9361 caaaagtatc ccttggtatc tgccttgagc cgccctctga tttctaaaaa attggttgaa
     9421 atagcgcatc agataggagc gactacaatt gctcatggtt gtaccggtaa ggggaatgac
     9481 caagtacgtt ttgaagtatc gattgcagcc ttggatccca atttaaaagt gattgctcca
     9541 gttcgtgaat ggaaatggtc tcgggaggaa gaaatttatt atgccaagaa aaatggggtg
     9601 cctgttcctg ctgaccttga caatccctac tctgtcgacc aaaatctttg gggacgtgcc
     9661 aatgagtgtg gtattttgga aaatccatgg aatcaggctc cagaagaagc ctttggcatc
     9721 acaacttctc cagagcaagc tccagatatg ccagaataca ttgagattga gtttagtgaa
     9781 ggtgttccag tttccctcaa tggagaagtg ttaaaattgg cagatttgat tcaaaaactc
     9841 aatgaaatag ctggaaaaca tggggttgga cggattgacc acgtggaaaa tcgcttggtt
     9901 gggattaaat caagagaaat ctatgagtgt ccaggtgcag tgaccctgct gacagctcat
     9961 aaggaaattg aggacttgac gcttgtgaga gaagtggctc attttaaacc aatcatagaa
    10021 aatgaattat caaatctcat ttataatgct ttgtggttta gttcggctac acaagccttg
    10081 attgcttata ttaaagagac tcaaaaagtt gtcaatggga ctgcaaaagt caaactgtat
    10141 aagggggagc gctcaggtcg tggctcggaa atcaccaaat tctctttacg atgaaaattt
    10201 ggcgacgtat actagtgcag atacctttga ccaagatgcg gctgttggct ttattgagtt
    10261 atggggactt ccaaccaagg ttcat
//
