LOCUS       NZ_CPTT01000011        10297 bp    DNA     linear   CON 13-DEC-2024
DEFINITION  Streptococcus pneumoniae strain 10F/2, whole genome shotgun
            sequence.
ACCESSION   NZ_CPTT01000011
VERSION     NZ_CPTT01000011.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  AUTHORS   Informatics,Pathogen.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2015) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001138385.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 01:30:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,083
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 60 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 32 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:01:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 10210
            Orig. end                         :: 20507
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CPTT01000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5297](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5297
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            762..896
                     /gene="rpmH"
                     /locus_tag="AEH42_RS05780"
                     /old_locus_tag="ERS011481_01124"
     CDS             762..896
                     /GO_component="GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA]"
                     /GO_component="GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /locus_tag="AEH42_RS05780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01124"
                     /product="50S ribosomal protein L34"
                     /protein_id="WP_000831905.1"
                     /transl_table=11
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA"
     gene            <1102..2061
                     /locus_tag="AEH42_RS05785"
                     /old_locus_tag="ERS011481_01125"
                     /pseudo=""
     CDS             <1102..2061
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_367670414.1"
                     /locus_tag="AEH42_RS05785"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01125"
                     /product="DUF1542 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="KVEVGGDGTATIVFKDGSAITIPGNQLVAQDPKAQDSTKPTAEKS
                     TVKAPAQRVDVKDITHLTDEEKVKVAILQANGSALDGATINVAGDGTATITFPDGSVVT
                     ILGKDTVQQSAKGESVTQEATLEYKLETIPGGDKGGNTGSSDANANEGGGSQAGGSAHT
                     GSQNSAQSQASKQLATEKESAKNAIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQA
                     ALKEIENAKTMEDVKEAETIGVQAIVMVTVPKRPVAPKTTSAPQATAGTMQDVTYQSPA
                     GKQLPNTGSASSAALASLGLVVATSGFALLGRKTRRRK"
     gene            2366..3139
                     /locus_tag="AEH42_RS05790"
                     /old_locus_tag="ERS011481_01126"
     CDS             2366..3139
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /locus_tag="AEH42_RS05790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01126"
                     /product="TatD family hydrolase"
                     /protein_id="WP_000575617.1"
                     /transl_table=11
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIER
                     ALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTAPK
                     EVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLEWAE
                     KFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENKTAYT
                     RYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            3139..3699
                     /gene="rnmV"
                     /locus_tag="AEH42_RS05795"
                     /old_locus_tag="ERS011481_01127"
     CDS             3139..3699
                     /EC_number="3.1.26.8"
                     /GO_function="GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rnmV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /locus_tag="AEH42_RS05795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01127"
                     /product="ribonuclease M5"
                     /protein_id="WP_000660005.1"
                     /transl_table=11
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERIQ
                     RLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEHAS
                     YEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSNGKQ
                     LLKRLELFGVTLAEVEEAMKSYE"
     gene            3771..4681
                     /locus_tag="AEH42_RS12450"
                     /pseudo=""
     CDS             3771..4681
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /locus_tag="AEH42_RS12450"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="XRE family transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPSL
                     PKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEIYK
                     TFIKHTLIIFLKKNN"
     misc_feature    4752..4761
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    4838..4851
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.05"
                     /tool="antismash"
     gene            5001..5297
                     /locus_tag="AEH42_RS05805"
                     /old_locus_tag="ERS011481_01130"
     CDS             5001..5297
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /locus_tag="AEH42_RS05805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01130"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000815392.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.2e-21,
                     bitscore: 68.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNGG
                     AYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            5374..7404
                     /locus_tag="AEH42_RS05810"
                     /old_locus_tag="ERS011481_01131"
     CDS             5374..7404
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /locus_tag="AEH42_RS05810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01131"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_050119432.1"
                     /transl_table=11
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMINQ
                     PVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSKES
                     AETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLLSLA
                     IFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSLLGLG
                     ILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDRLKGKLPLKR
                     IMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGWSSAFAD
                     EEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTPSGNVIYV
                     SPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYLQNFSSESV
                     EVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQATGTRPVAGML
                     WGTTANSALKLDRYGDSITALKEKGLYHKVSYLVKSQLFFAKVLNDKRVEFYSLLIGTI
                     LTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGGVLLLGLVLSSI
                     LTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            7406..8047
                     /locus_tag="AEH42_RS05815"
                     /old_locus_tag="ERS011481_01132"
     CDS             7406..8047
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /locus_tag="AEH42_RS05815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01132"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000565531.1"
                     /transl_table=11
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLLN
                     ILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFVGQ
                     KISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILADEPT
                     AALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            8040..8333
                     /locus_tag="AEH42_RS05820"
                     /old_locus_tag="ERS011481_01133"
     CDS             8040..8333
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /locus_tag="AEH42_RS05820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01133"
                     /product="hypothetical protein"
                     /protein_id="WP_000333255.1"
                     /transl_table=11
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYLL
                     DFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            8584..9456
                     /gene="rsmA"
                     /locus_tag="AEH42_RS05825"
                     /old_locus_tag="ERS011481_01134"
     CDS             8584..9456
                     /EC_number="2.1.1.182"
                     /GO_function="GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rsmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /locus_tag="AEH42_RS05825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_01134"
                     /product="16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="WP_001216858.1"
                     /transl_table=11
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEID
                     DQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKV
                     DLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRISAQ
                     PNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFF
                     FKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGL
                     ADALKGQGL"
ORIGIN
        1 acgatttgat tatctttgcg gatgaaatct atgaccgcat ggtaatggac ggacatgtgc
       61 atacgcctgt ggcgagcttg gcaccagatg tcttctgtgt cagcatgaat ggtctgtcaa
      121 aatcccaccg catagcaggt ttccgtgtgg gttggatggt cttgtctggc cctaagactc
      181 atgttaaggg ctatatcgaa gggctcaata tgctgtccaa tatgcgcctt tgctctaacg
      241 ttttggccca acaagtcgta caaacttcct tggggggtca ccaatcagtc gatgaattgc
      301 ttcttcctgg tggacgaatc tacgagcaaa gaaatttcat ctataatgcc attcaagata
      361 ttccaggttt gtctgccgtt aaacccaagg cggggctcta tatcttccca aaaatcgacc
      421 gcaatatgta ccgtatcgat gatgatgagc agtttgtcct tgatttcttg aagcaggaaa
      481 aggttctctt ggttcatggt cgaggcttta actggcagga accagaccac ttccgtatcg
      541 tttaccttcc tcgtgttgat gagttagccc aaatccaaga gaagatgact cgtttcttga
      601 aacagtatcg tagatagggc ttgcattcga aaaagctgga aacatttgcc tagagctatt
      661 gacaaaagtg aaagaatcag atagaatagt aaagtatgtt tagtaactga tcactttata
      721 gccggctaaa cgaatacaaa atctatgagg aggtattcat cgtgaaacgt acttatcaac
      781 caagtaaact tcgtcgtgcg cgtaaacacg gattccgtaa ccgtatgtca actaaaaatg
      841 gtcgtcgcgt attggcagct cgtcgtcgta aaggacgcaa agttttggct gcataatcca
      901 aacgaattct atcaaaaatc agtaggaact cgagtctact gatttttatt tttgtaaaaa
      961 agttcagtag atgcaaatgg attcggaagc gatgttatag tagattgaaa ctagaatagt
     1021 acacctctac ttctaaaaca tttttagaat tagattttac tgtcctgatc gatttgttct
     1081 gttattattt tattttacta taaagttgaa gtaggtggag atggtacagc aacaatcgtc
     1141 tttaaagatg gttcagctat tacaattcca ggaaatcaat tggtagcaca agatccaaaa
     1201 gcacaagata gcactaaacc gactgctgaa aaatcaactg ttaaagcacc tgctcaaaga
     1261 gtagatgtaa aagatataac tcatttaaca gatgaagaaa aagttaaggt tgctatttta
     1321 caagcaaatg gttcagcatt agacggagcg acaatcaatg tagctggaga tggtacagca
     1381 acaatcacat tcccagatgg ttcagtagtg acgattctag gaaaagatac agttcaacaa
     1441 tctgcgaaag gtgaatctgt aactcaagaa gctacactag agtataagct agaaactata
     1501 ccaggtggag ataagggagg caatactgga agctcagatg ctaatgcgaa tgaaggcggt
     1561 ggtagccagg cgggtggatc agctcacaca ggttcacaaa actcagctca atcacaagct
     1621 tctaagcaat tagctactga aaaagaatca gctaaaaatg ccattgaaaa agcagccaag
     1681 gataagcagg atgaaatcaa aggcgcaccg ctttctgata aagaaaaagc agaactttta
     1741 gcaagagtgg aagcagaaaa gcaagcagct ctcaaagaga ttgaaaatgc gaaaactatg
     1801 gaagatgtga aggaagcaga aacgattgga gtgcaagcca ttgtcatggt tacagttcct
     1861 aagagaccag tggctcctaa gacaacaagt gcaccgcaag caactgcagg aacaatgcaa
     1921 gatgttacct accagtcacc tgctggcaaa caattaccta acacaggttc agcatcaagt
     1981 gcagcacttg ctagtcttgg tctagtggtg gcaacaagtg gttttgcttt gctaggaaga
     2041 aagactagac gtagaaaata gaacagctag aaaattctat tctctactta aagttagatt
     2101 ataaggggga ttttgagaag tcatcaatcc tagtgatggg tgagaaaagt gagaacccaa
     2161 gataatcaca tactttagct gaataggaat attctatcaa tgtagccaat ctcttctgtc
     2221 tctaactgtg gaataggaga tgggcaatat cggatagaaa agatagcaga atagctctct
     2281 attgaagaga ggaggggaaa ccgaaaaatt aggtgcccct cctctttttt ggtataatag
     2341 aagatagaaa acgaggttag aagagatgat ttttgataca catacacact tgaatgtaga
     2401 agaatttgca ggtcgtgagg cagaagaaat tgccttggct gctgagatgg gtgtgacaca
     2461 gatgaatatt gttggttttg ataaaccgac gattgagcgt gccttggagt tggtagatga
     2521 gtatgatcag ctctatgcga ctattggttg gcatcctaca gaagctggta cttatacaga
     2581 ggaagttgag gcttacttgt tggataagtt aaaacatccc aaggttgtgg ctttaggtga
     2641 aattggctta gattaccatt ggatgacagc gcccaaagag gtgcaggagc aggtttttcg
     2701 ccgtcagatt cagctatcta aggacttgga tttgcctttt gttgtccata cccgtgatgc
     2761 gctggaagat acctatgaga ttatcaagag tgagggcgtt ggtcctcgtg gtggtatcat
     2821 gcattcattt tcagggacgc ttgagtgggc agagaagttt gtggatcttg gtatgaccat
     2881 ttccttctca ggagtggtga cttttaagaa ggcaactgac ctccaagaag cagctaaaga
     2941 gttacctttg gacaagattt tggtggaaac agatgcgcct tacttagcac ctgtacccaa
     3001 gcgtggtcgt gaaaataaaa cagcctatac tcgctatgtg gtcgacttta tcgctgactt
     3061 gcgtggtatg acgacagaag agctggcggt agcaacgact gcaaatgcag aacgaatttt
     3121 tggattggac agcaagtaat gaaagagaaa atttctcaag ttatcgtggt tgaagggcgt
     3181 gatgatacgg tcaatctcaa acgttatttc gatgtggaga cctatgagac tcgaggttct
     3241 gccatcaatg ctcaggatat agagcggatt cagcgcctgc accaacgtca tggagtcatt
     3301 gtctttacag acccagattt taatggggag cggattcggc gcatgattat gacggccatt
     3361 ccaacagttc ggcatgcctt tctcaagcga gatgaagctg ttccaaagtc caagaccaag
     3421 gggcgttctc tgggaattga gcatgccagc tatgaagacc tgaaaacggc tctagctcag
     3481 gtgacagaac aatttgaaca tgagagtcag tttgacatta gtcgtagcga cttgattcgc
     3541 cttggttttc tagcaggggc agacagccgt aagcgtagag aatatctcgg agagactctc
     3601 cgaatcggct attctaacgg caagcaactc ctcaaacgcc tagagttgtt tggggttact
     3661 ttggcagaag tggaagaagc tatgaaatct tatgagtagg aaagatgtag ccgttacaat
     3721 tttttaagtt tcacagtatt tttcgaagca ggtagaagag gaggcgtctg atgttaattg
     3781 gtcaaaaaat taaagagatt cggatagaaa aaggaattag tcgtccagat ttttgtggag
     3841 atgagcaaga actgacagtt cgtcaactgt cgcgaattga aagtggagct tcgcaaccga
     3901 gtttgcccaa gttagcctat attgctcgcc ggctaggagt tccagtttat agccttatgc
     3961 cggatttttc agctcttcct cctgcttatt tagaattgaa ataccagatt ttacgtgaac
     4021 caatctatgg taaagaagag gagtacgata agaaggaagc gtgtttggaa gagatttata
     4081 aaacatttat aaaacatact ttgataatct tcctaaagaa gaacaattag catgtgaagt
     4141 attgcaggcg tgtttggata cttctagaac tagaaggcct gaatatgcag agttaatact
     4201 tgaggaacat atgcctcaga ttatagaaaa agaagcttat tcaataaatg atatgttgtt
     4261 gattcgtttg tttttttatc aaatgctcat tagaaaagat cttgccaaat ttataaatca
     4321 aatcgaaaag ctaatgctct ttcttttgga acagaagaag gtaactcaaa tagagaatta
     4381 ctttataatt agagatactc ttatttcagg aatgtgttgt cttgaaaagg taggagtaac
     4441 tgattgtttt aatgattatc tatcgtgttt acaagaaatt atggataaaa ctcaagatta
     4501 tcaaaagaaa cctcttgtat ttatgttttt gtggaagcaa gcattaagag tagaaagaga
     4561 ttttagttta gctgaatcat tttatcagtc ttctaaaaca tttgcgcagc taattggaga
     4621 tgaatttcta gtaaagaaat tgacagagga atggcaagag gatgtcaaaa aatatttata
     4681 aacatagtga atcagtgaca aaaatgtcct tgtcctcgta tcaaaacagt tctaaagttc
     4741 gtctttagaa ctgttttttt agatataagc taaaaataat atgaaatagt tagattttaa
     4801 ggacattgat gtccttgata gtgtgataaa gatggagtat aatgactata aaagttcatt
     4861 tcagggtgct attttgacaa tttagagaaa aacaaataat tttagaatat catttggtaa
     4921 tataatagta tcaacgttga taatggaaag aggagcaagt aactcaacaa tcttgtaatc
     4981 aaaaatagag gagtatcaat atgaaacaaa cagttaaaaa gttagctctt gtagcgagca
     5041 ttgcagcaac attaggtgga ggtgtatcag ttgcctctgc agccgttcaa tacccagaag
     5101 gtggtgtttg gacttatggt tcaggtaacg gtggtgctta ctcaaactac tatcaccctt
     5161 caaaatacca tagttcaaca gttgttagca gaaaaactgg ttcatctgac aagggatatg
     5221 ctggtgctgg agggacttct cgtgcatgga ttcgtacttc ttggggagag aaagttgcat
     5281 tctactataa tgtttagaat ggtatagccc tattgtttag ctttacttgg tcaccttctc
     5341 actaagtgac caagtaactt tttggaggaa atgatgaaac gtttatttat tttgatttca
     5401 atggtattag tatcgcttta tatggtgata acttccgttg accatcgaga agagatttta
     5461 tttggtaact atccttctgt tgatgtgaca ggaatgatga taaatcaacc agtagctagt
     5521 cgcgaagagg tgacagaggc tttgagtcac ttggcggtag agcacaatag tctcattgct
     5581 cgtcgaatcg ttgagccaaa tgaagctgga gaaacacgct ttacctatgc cacttatggt
     5641 gagggaaagc ttccagaagg tctgaccatt tcctccaagg agagtgcaga aacgagtgat
     5701 ttattagggt cttacttgat tgtatcagga agtttggatg gagtgagctt acagaccacc
     5761 ttgaaagagc ttggttatca aggctttgtt tcgaatggag aagatccatt ttcgatagtc
     5821 ttactattga cggccacccc tatggtgcta ctgagtttag ctatttttct gctgaccttt
     5881 atgagtctga ccctgattta tcggatcaaa tcccttcgtc aggcagggat tcgcttaata
     5941 gctggtgaga gcttgtttgg agttgctctc agaccagtgt tagaagatgt gagacagctt
     6001 atctgctcag tgctggtatc cagtcttttg ggattgggga ttctctggta tcaaggtgcc
     6061 ttgtttatgg caacggtgca actggtcatc attgctcttc tactttatgg attgaccctg
     6121 gcagggattt ctaccttact aagtgtcgtc tatctacttg gtttacagga aaatagtctg
     6181 gtggatcgat tgaaagggaa actccctctc aaacgtataa tgacattgat gatggtgggg
     6241 caactcttag ctgtattggt ggtcggatcg agtgcgacag ctctcctacc ccactaccgt
     6301 gaaatgcagg aaatggagag agctagcaat aaatggagcc agtcctcaga ccgttaccgt
     6361 ctatcctttg gttggtctag tgcatttgcc gatgaagaag gaacgcgtaa ggataatcgt
     6421 gagtggcaga catttactga agaacggtta gccaatacag actcttttta tattatgagc
     6481 aatgttgaca atttctcaga tggagcagaa gtggacctag atggcaatcg tctcagtgac
     6541 tacacaccgt cagggaatgt tatctatgtc tcaccacgct atctgataga agaaaagatt
     6601 accgtttctt cagagtttat ggacaagatg caaaacttgt ctgagggaga gtttgggctg
     6661 atcttgcctg agagcttgcg agagcagtct gtctactacc aaggattgtt tacagattac
     6721 ctgcaaaact tttcatctga aagtgtagaa gtgacgagtc agaaacacta cctcccacag
     6781 gtaaggctag cttttacaga aacaggacag gaacgtttcc tctataatga tgggtacaag
     6841 acaacacgcc agtacctaaa agatccgatt attgtagttc taacgccgca agcgactgga
     6901 acaagacctg ttgcagggat gttgtgggga actacggcta atagtgcctt gaaactagat
     6961 cgatatggag acagcatcac agctctaaaa gagaaaggtc tgtatcacaa ggtttcttac
     7021 ttggtaaaaa gccagctatt ttttgccaag gtactaaatg acaaacgggt ggagttttac
     7081 tctctcctta ttgggacgat tttgaccctg tctacggcta tcttgttatt tgattccatg
     7141 aatcttctct attttgagca gttcagacgg gaacttatga ttaaacgtct tgctggtatg
     7201 acaatctatg agcttcatgg caagtattta ctggcgcaag gaggagttct cttgcttggc
     7261 ctagtcctat ctagtatttt gacaagagat ggtttgatta gcgctctagt tgtagctttg
     7321 tttacgctta acgccctctt gattttagta aggcaggaca aaaaagaaga agctggtagc
     7381 atggcagtat tgaaaggaaa ataagatgat tgatattcaa ggattggaaa agaaatttaa
     7441 tgaccgcgcg attttctctg gtttgaatct caagctggag aagggcaagg tttatgcctt
     7501 aatcggaaag agtggaagcg gaaagacgac gctgctgaat atcttgggaa agctagaaaa
     7561 gatagatggt ggaagggttc tctatcaggg gaaagattta aaaaccattc ccactcgtga
     7621 gtattttcga gaccagatgg gctatctctt tcaaaatttc ggcctcttag aaaaccaatc
     7681 aatcaaagaa aatttggatt tgggttttgt tggtcagaaa atctcaaaag tagaacgttt
     7741 ggaaaggcaa gtgggggctt tagaaaaagt taatctaggg tatttggatt tagaacaaaa
     7801 aatctatact ttatctgggg gagaggccca acgagttgcc cttgctaaga ctattttgaa
     7861 aaatccaccc ttgattttgg cagatgaacc aacagcagct cttgatcctg aaaattcaga
     7921 ggaggttatg aatctcttgg tggatttgaa agatgaaaat cgaattatca tcattgcgac
     7981 ccataatccc ctagtctgga ataaggctga tgaaatcatt gatatgagga aacttgctca
     8041 tgtgtgaaaa aatccgtatt cgcagggtat ctgattatcc tagtgccaga ggtggtttag
     8101 aagatatcct catcatggaa aatatgacca atcatctcct tttggttcaa atccgagtgc
     8161 atggctattt gcttgatttt gctagtattg aagggcaaag gcaaaagcat tatcgtttga
     8221 aaaatttacc tcagacggtt gaactgacag tggatgatgt ggaggaggat gtggatttga
     8281 ccctacctga aaatcgaagt tatcaagaag ctgatttttt tgatactcaa tgaaaatcaa
     8341 agagcaaact aggaagctag ccgcaggctg tacttgagta cggcaaggtg aagctgacgt
     8401 ggtttgaaga gattttcgaa gagtatgaac gtatgtttcg agagaagcgc taagtccctc
     8461 tttaaagatt tccaagacta tctttcttca tgaggaaaga tagttttttg gtatgatttt
     8521 cattcccaaa atacaagggg aatgtgttac aatagtagta acagataata gaaaagagaa
     8581 tagatgagaa ttgcagatta tagcgtgacc aaggcagtgc tggagcgtca cggttttacc
     8641 tttaaaaagt ccttcgggca aaatttcctg acggatacca atatccttca aaaaattgtg
     8701 gatacggctg aaattgatga ccaggtcaat gtcatcgaaa tcgggccagg tattggtgcc
     8761 ttgacagaat ttttggctga gcgtgcagcc caagtcatgg cttttgagat tgaccaccgt
     8821 ttggtgccaa ttttggcaga tactctgcgt gattttgata atgtgaccgt agttaacgaa
     8881 gatattctca aggttgattt ggcgcaacat atccagaatt ttaaaaatcc tgacctgcca
     8941 atcaaggtag tggctaattt gccttactac atcacgacgc ctattctcat gcacttgatt
     9001 gagagtggga ttccttttag tgagtttgtg gtcatgatgc agaaagaagt agcggaccgc
     9061 atttcagccc agcctaacac caaggcttac ggtagcttgt ctatcgccgt gcagtattac
     9121 atgacagcca aggttgcctt tatcgtgcct cgtacggtct ttgtgccagc gccaaatgtg
     9181 gattcagcca tcttgaaaat ggtgcgccgt ccagagccag ctgtagcagt ggaagatgag
     9241 aacttcttct ttaaggtttc caaggctagt tttacccatc gccgtaagac cttgtggaat
     9301 aacttgacag gttactttgg taagactgaa gaggtcaagg acaagctgac caaggctttg
     9361 gaccaggcag gcttgtcacc aagtgtgcgt ggggaagctc tcagcttggc agaatttgcc
     9421 ggtctagcag acgcacttaa agggcaagga ctctaagatg cagggacaaa tcattaaagc
     9481 cttggcaggt ttctactatg tggagagtga tggccaggtt tatcaaacac gcgcgcgtgg
     9541 gaatttccgt aaaaaaggcc atacccctta tgttggggac tgggtggatt tctctgccga
     9601 ggaaaattca gaaggctata tcctaaaaat tcacgaacgg aaaaacagtc tggttcgtcc
     9661 gcctattgtc aatatcgatc aagctgtagt aatcatgtcc gtcaaggaac ctgattttaa
     9721 cagcaatttg ctggatcgtt tcttggttct tttggagcac aagggcatcc atcccattgt
     9781 ctatatttcc aaaatggatt tgttggaaga taggggagaa ctggattttt accagcagac
     9841 ctatggtgac atcggctatg actttgtgac cagtaaagag gaactcctgt ctttgttaac
     9901 aggcaaggtt acggtcttta tggggcagac aggtgttggg aagtcaactc ttctcaataa
     9961 aatcgcacca gacctcaatc ttgaaacggg agaaatttca gacagtctag gtcgcggtcg
    10021 ccataccact cgagctgtta gtttttacaa tctcaacggg ggtaaaatcg cagatacacc
    10081 aggattttca tccttggact atgaagtatc aagggctgaa gacctcaatc aggctttccc
    10141 agagattgct actgttagcc gagattgtaa gttccgtact tgtacccata cccatgagcc
    10201 gtcttgtgcc gtcaaaccag ctgttgaaga gggtgttatt gcaaccttcc gttttgacaa
    10261 ttacctgcaa ttccttagtg aaattgaaaa tcgtaga
//
