LOCUS       NZ_CPTT01000003        10867 bp    DNA     linear   CON 13-DEC-2024
DEFINITION  Streptococcus pneumoniae strain 10F/2, whole genome shotgun
            sequence.
ACCESSION   NZ_CPTT01000003
VERSION     NZ_CPTT01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  AUTHORS   Informatics,Pathogen.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2015) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001138385.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 01:30:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,083
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 60 of 139
            Pseudo Genes (incomplete)         :: 78 of 139
            Pseudo Genes (internal stop)      :: 32 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:01:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 65643
            Orig. end                         :: 76510
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CPTT01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10867
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10867
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10867
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5867](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5867
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(657..1532)
                     /locus_tag="AEH42_RS02160"
                     /old_locus_tag="ERS011481_00421"
     CDS             complement(657..1532)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /locus_tag="AEH42_RS02160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_00421"
                     /product="substrate-binding domain-containing protein"
                     /protein_id="WP_000669493.1"
                     /transl_table=11
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISRENG
                     SGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSLTK
                     SVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVVTDN
                     KFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNGSSAG
                     ITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAELADVFS
                     GKLTTWDKIK"
     gene            complement(1699..3030)
                     /gene="pnpS"
                     /locus_tag="AEH42_RS02165"
                     /old_locus_tag="ERS011481_00422"
     CDS             complement(1699..3030)
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene="pnpS"
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /locus_tag="AEH42_RS02165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_00422"
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="WP_000833268.1"
                     /transl_table=11
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGGY
                     WLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKDLA
                     ETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLVHQA
                     FDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLNLAFV
                     SNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLLTLSKA
                     QQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQILLNLL
                     SNAIRYTEQGGKIEVKTQKGNEGIKISVSDTGIGISQLEQDRIFERFYRVNKGRSRQTG
                     GTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(3031..3738)
                     /locus_tag="AEH42_RS02170"
                     /old_locus_tag="ERS011481_00423"
     CDS             complement(3031..3738)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /locus_tag="AEH42_RS02170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_00423"
                     /product="response regulator transcription factor"
                     /protein_id="WP_000166475.1"
                     /transl_table=11
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAETE
                     PFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLTKP
                     FSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLSLTP
                     KEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKAPQFI
                     RTIRGYGYKFKEL"
     gene            complement(3822..4307)
                     /locus_tag="AEH42_RS02175"
                     /old_locus_tag="ERS011481_00424"
     CDS             complement(3822..4307)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /locus_tag="AEH42_RS02175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_00424"
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001826677.1"
                     /transl_table=11
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYT
                     SIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHY
                     CVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            <4293..4851
                     /locus_tag="AEH42_RS02185"
                     /old_locus_tag="ERS011481_00425"
                     /pseudo=""
     CDS             <4293..4851
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /locus_tag="AEH42_RS02185"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="ERS011481_00425"
                     /product="transposase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="IRRSQWVEVTLVQSGVTISRTPLSSEDTVMIDATEVQINRPKKRI
                     SELF"
     gene            5001..5255
                     /gene="blpM"
                     /locus_tag="AEH42_RS02190"
                     /old_locus_tag="ERS011481_00426"
     CDS             5001..5255
                     /codon_start=1
                     /gene="blpM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /locus_tag="AEH42_RS02190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_00426"
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="WP_000379879.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 4e-20, bitscore:
                     65.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGLG
                     TAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            5271..5474
                     /gene="blpN"
                     /locus_tag="AEH42_RS02195"
                     /old_locus_tag="ERS011481_00427"
     CDS             5271..5474
                     /codon_start=1
                     /gene="blpN"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /locus_tag="AEH42_RS02195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_00427"
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="WP_001099492.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.2e-13,
                     bitscore: 43.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGPW
                     GAAIGGIGGAVVCGYLAYTATS"
     gene            5718..5867
                     /gene="blpO"
                     /locus_tag="AEH42_RS02200"
                     /old_locus_tag="ERS011481_00428"
     CDS             5718..5867
                     /codon_start=1
                     /gene="blpO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /locus_tag="AEH42_RS02200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_00428"
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="WP_001818346.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 3.4e-11,
                     bitscore: 36.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDGC
                     ATTV"
     gene            <5903..5968
                     /locus_tag="AEH42_RS12420"
                     /pseudo=""
     CDS             <5903..5968
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863320.1"
                     /locus_tag="AEH42_RS12420"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ComC/BlpC family peptide pheromone/bacteriocin"
                     /pseudo=""
                     /transl_table=11
                     /translation="VTGAAGGAILGGVAYAATCWW"
     gene            5971..6090
                     /locus_tag="AEH42_RS02205"
     CDS             5971..6090
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /locus_tag="AEH42_RS02205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="WP_000346296.1"
                     /transl_table=11
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     misc_feature    complement(6226..6239)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.08"
                     /tool="antismash"
     misc_feature    6309..6322
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.42"
                     /tool="antismash"
     misc_feature    6436..6456
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 24.73"
                     /tool="antismash"
     gene            6876..7259
                     /locus_tag="AEH42_RS02215"
                     /old_locus_tag="ERS011481_00429"
     CDS             6876..7259
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /locus_tag="AEH42_RS02215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_00429"
                     /product="hypothetical protein"
                     /protein_id="WP_016398494.1"
                     /transl_table=11
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGVF
                     LLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHIFI
                     VFNVLMYLLSSSQVSLKKNKLSS"
     gene            7311..8000
                     /locus_tag="AEH42_RS02220"
                     /old_locus_tag="ERS011481_00430"
     CDS             7311..8000
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /locus_tag="AEH42_RS02220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_00430"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_050119095.1"
                     /sec_met_domain="Abi (E-value: 4.8e-15, bitscore: 49.0,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFVW
                     IQNILSLLFSGVMIWILAKTGHGYLFRIPRKKWLWYSILTVLVLVLHISFNVQIAKHVQ
                     STAEGWAVLIGYSGTNFAELGIYVTLFFLTPLMEELIYRGLLQHAFFKHSRFGLDLFLP
                     SILFALPHFSRLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFPFLLT
                     FLHRVLG"
     gene            8042..8298
                     /gene="blpZ"
                     /locus_tag="AEH42_RS02225"
                     /pseudo=""
     CDS             8042..8298
                     /codon_start=1
                     /gene="blpZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /locus_tag="AEH42_RS02225"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="immunity protein BlpZ"
                     /pseudo=""
                     /transl_table=11
                     /translation="MYKHLFF"
     gene            8424..9035
                     /locus_tag="AEH42_RS02230"
                     /old_locus_tag="ERS011481_00431"
     CDS             8424..9035
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /locus_tag="AEH42_RS02230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_00431"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_044814721.1"
                     /sec_met_domain="Abi (E-value: 4.9e-21, bitscore: 68.2,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSLL
                     VTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHLAE
                     VSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSNVPS
                     FIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLTYL"
     gene            9197..9991
                     /gene="ccrZ"
                     /locus_tag="AEH42_RS02235"
                     /old_locus_tag="ERS011481_00432"
     CDS             9197..9991
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /GO_function="GO:0004747 - ribokinase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /GO_function="GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /GO_process="GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ccrZ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /locus_tag="AEH42_RS02235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_00432"
                     /product="cell cycle regulator CcrZ"
                     /protein_id="WP_000363002.1"
                     /sec_met_domain="APH (E-value: 2.8e-19, bitscore: 63.1,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGLA
                     REQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQLS
                     RLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDVRHS
                     NWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTVLSKL
                     YWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            9988..10623
                     /gene="trmB"
                     /locus_tag="AEH42_RS02240"
                     /old_locus_tag="ERS011481_00433"
     CDS             9988..10623
                     /EC_number="2.1.1.33"
                     /GO_function="GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="trmB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /locus_tag="AEH42_RS02240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS011481_00433"
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="WP_001266083.1"
                     /transl_table=11
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVEV
                     GSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTDYF
                     EDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFEYSL
                     VSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
ORIGIN
        1 gccaagctac cagaataata cgttttggga actgttcgga tagcagattc tgacaaactg
       61 ataatggttg gcaaaatcat tattcctaat agtaacgaag cggttaggac actcatgcca
      121 tttcctaaaa agcttctaat ccaaggcacc aataattgta ggccgaaaaa accataaaca
      181 atagatggaa tggccgccat caagttgata gctgatttta agaagccata gacgggcttt
      241 ggacaataat aaaccataaa cactgatgtc aagatgcctg ttggcacccc aatcacaatc
      301 gctcctaagg taattaataa ggaaccaacg atcattggta aaataccata gcttgccgga
      361 atgttcgttg gcgaccaatc actgcctaat aaaaaacggg caaagccgta gttagccatg
      421 aaaggtaagc cattactaaa aataaagaaa cagattagca aaatagctac aacagctact
      481 gttgcactca tgaaaaaaat tgccctaaaa actgcttctt tgaaggcttg ttttgtcaca
      541 tcttgtcctt tctagtgaag aaagtaaggg agatacgaca cctccctact tgccttcttt
      601 atcttattgt acgatgaaac gtctgcatct ctttagagat ttatggagca aacattttat
      661 ttaatcttgt cccaggtggt taatttgcca ctaaaaacgt ctgcaagttc agccatactg
      721 acttggcttg ccttattgtc attattgacc acaacagcaa taccgtctaa agcaatagca
      781 tcatgggtga gactcttacc ttcttcagga gttaattccc tagaaaccat accaatatca
      841 gcggttttct ccttaacagc ggtaatacct gctgaagacc cattagaggt aatatcaatc
      901 gtaacttctg gattttcttt tttataagct tctgctaatt tttccattaa agaagatact
      961 gaagtggaac ctacaacaga caacttgcct gataagtgtt ggcttgtata ttctgtggtt
     1021 tcggttttag cttcaataaa tttattatct gtgaccactt gttgaccttg tttggagtgg
     1081 ataaagctga taaaatcttg acctagcttg gaaagattag aagaccaaac aatgttgaag
     1141 ggacgttgaa gagggtattc accatctaaa actgtgtctc gactagcctt gacaccatca
     1201 atctctaaag ccttgacaga tttcgttaaa gatcccaagg agatgtagcc gatagcatta
     1261 gcattccctt gaactgctga gagaacacct tctgtactat tttgaatcac agctgttttg
     1321 gcagtgttgt caattttttt atcaccgtct tttttgagaa tccctgtgat ttctgtgaag
     1381 gcaccccgtg ttccagagcc attttctcgt gaaatcacct caatcgttcc tgaagctgac
     1441 tgtttggaag cagctgactg attgccacag gcaacaagcc caaatcctga taagccaatg
     1501 gctgcaagag taagcatttt tttgaatttc ataataatca cctttatctc tatgtatttt
     1561 tcttgtgtag gcttactaca tttatagtct aacaagtctt tgtaaaggtt tatccctgat
     1621 tcatgtaaag attgtgtaaa gaatcaaaaa aagccacttt tgaaaaatgg ctgctcctaa
     1681 aaatagcttt aaaaattatt agtcctgtgc gaaagattgg ttaggaagaa aaatcgtgaa
     1741 gcaactgcct ctgccaagct gactcgtcac cgtgacttgg ccacctaata attgactgag
     1801 ttctttgaca atggcaaggc caagaccagt gccaccagtt tgtctgcttc gacctttatt
     1861 aactcggtaa aaacgttcaa aaatacgatc ctgctctaat tgactaatac caatccctgt
     1921 atctgataca gaaatcttaa tgccttcgtt ccccttttgg gtcttgacct caatttttcc
     1981 cccttgttca gtgtaacgga tggcattgga taaaagattg agtaagattt gggaaagtaa
     2041 ttgactatct gatacgaggg tgacatcatc tggcacctgc acctttagct gtaaatcctt
     2101 cttcttgagc tgaggttgca agctttgagt caaatcctgt acaaattctg ccaaagaaag
     2161 ggtcgtccat tgtataggca tttgttgagc cttagataag gtaagaagat gctcaacaat
     2221 atgctcaaga cgcaaacttt ctttgtaaat aatgtctaga aagtcatcct tgagcgcttc
     2281 ttcttcagct gacatcccct taatggtttc agcaaagccc ttaatcgaag taactggtgt
     2341 cctcaattca tgggaggcat ttgagacaaa ggctaaattt aacttttcat aagttctaat
     2401 cgttgttaaa tcatatagca agacgagcac agcttccaca gattgggtgg ggctaaaaac
     2461 gggaactgct gtcacttcta aaatcaagtc accctcatga aacccactta cttcttgttt
     2521 taaccttgtt ttttgatcaa aggcttggtg aactaaattt cgaatatcca tccgtttgag
     2581 gtcatcaagt gaacttatgt cgccgtccac atcgggaaaa taatgaggca gagagcgact
     2641 ggataataac atctgacctt gagcggaaac taaaaacgtc cccatggtta ggtgcgacag
     2701 aagaacctcc attgtttcgg ctagatcctt gtattgctga tcctgttggg agactttggt
     2761 ttttaggcca gacacatact gagccaaaga ctttaagtct tcttgccctt tttctaaaaa
     2821 gtattcacta ctggtcaaga gaggttggtg caaggtctca aaagcaactt cccatttcca
     2881 aaggcaaaag agccagtagc cacctagtcc caaagaaagg gctagaagaa agagaccgat
     2941 gcctttactg atccaagtta atgccatccc tgcaatcaga atgaggctaa cacttagatt
     3001 gactagccaa aattgaaggt agcgtttcat ctataactcc ttgaacttat aaccataacc
     3061 ccgaatggtt cgaataaatt gaggggcttt aggattgtct tcaatttttt ccctcaactt
     3121 accaatatga acgtccacca aacgtgtttc ctgcccaaag tcataccccc agatacgttc
     3181 caaaagacgc tctctagtca gtgtcatgtt gggatgtttc ataagataga gcaagagttc
     3241 aaattctttt ggggtcaaac tcagtaactt attcgccttg tagacttcat gacgctcagg
     3301 gtatactttc aaggtcccaa atagccaaga atcgtcagcg atattatctg aatcatctcc
     3361 ttcttgttct cctttagttc gcctgaggac agccttgaca cgcgccagca attctctagg
     3421 gctaaaaggc ttggtcaggt agtcatcagc ccctaattcc aaggccaaaa ccttatcaaa
     3481 ttcatcactt ttcgcagaaa ccatcataat tggagttttg acgcctttgg ctctcagccg
     3541 cttacaaact tccatgccat ctaattgtgg taacatgata tcaagcaaga taaaatcaaa
     3601 gggttctgtt tctgccaaag ctaaggcctt ccgtccattt gtcaccaatt gagtagaaaa
     3661 gccttcctta cttaaatggt agtcaagcaa tttcagaatg tgttcttcat catccactaa
     3721 taagacttgt tttgtcatct attatctcct attggtaaca ttataacaca attatcagaa
     3781 atcctaacat tgctaaatca gattaaattt gcctatcaag actagtatct ggtcaaacgc
     3841 tcaatcatct ccttgtgctc tggataggtc gccagtagat ctaccctttc aaataattca
     3901 aaatcctcaa attcaaaacc aggagcaaca agacaagaaa ccagagcatc atccttatca
     3961 actgttgatc cccaaatagt gcccttagga acacagtagt gaagttgttg ccctttggat
     4021 atgtccaggc ctaaagtgac tgcttcgtag tgaccatctg ctgtaatcat gtgaacagta
     4081 agtggggatc ctgcatgaaa ataccagatt tcatctgctg tcaatcggtg aaaatgtgaa
     4141 ggattcgttt cttctaataa gaaataaata ctggtataaa gcgcccttcc cttaccagca
     4201 aggtttatag tgtctgaagc tttttttgtt tgtctaaaat agccaccttc aacatgggga
     4261 gctaactcta gagttcttat caagtcttct ttatccgtcg gagccaatgg gttgaagtaa
     4321 ctcttgttca aagtggtgtt acgatttcaa gaactcctct cagttctgag gacacggtaa
     4381 tgattgatgc gacggaagta caaatcaatc gccctaaaaa aagaattagc gaattattct
     4441 gataaaaaaa aatgccacgc tatgaaggct caagcgattg tcacaagtca agggagaatt
     4501 gtttctttgg atatcgctgt gaactattgt catgatatga agttgttcaa aatgagtcgc
     4561 agaaatatcg gacaaactgg taaaaccttg gctgacagta attatcaagg gctcatgaag
     4621 atatatcctc aagcacaaac tccacgtaaa tccagcaaac tcaaaccgct aacagttaaa
     4681 gataaagcct ataaccatgc gttatctaag gagagaagca aggttgagag catccttgcc
     4741 aaagtaaaaa cgtttaaaat gttttcaaca acctatcgaa atcatcgtaa acgcttcgga
     4801 ttacgaatga atttgattgc tggtattatc aatcatgaac taggattcta gttttgcagg
     4861 aagtctaatg taatttttgc aattcaagac gtttcgatga ctattcaaga tctggatgaa
     4921 aaattttaaa aaacgatgat atactaaact tgtcaaagtt acaacaagac aaaaattaaa
     4981 aataaaaaag agtatttgtc atggatacaa aaattatgga acaatttcat gaaatggata
     5041 taactatgtt atctagtatt gaaggaggca agaataattg gcaaactaat gtcttagaag
     5101 gtggtggtgc tgcttttggt ggctgggggc tagggacagc tatttgtgct gcgagtggtg
     5161 ttggagcacc atttatggga gcatgtggat acataggagc taaatttggt gtggatcttt
     5221 gggcaggagt aacaggggca acgggtggat tttaataaaa ggagacttgt atgaatactt
     5281 attgtaatat aaatgaaaca atgctgagtg aggtttatgg aggtaattcc ggaggagcag
     5341 ctgtagttgc tgctttaggt tgtgcagcgg gtggagtgaa atatgggaga cttctaggac
     5401 catggggcgc tgcaatagga ggaattggag gagcagtggt ttgtggatat ttagcctata
     5461 ccgctacatc ataaaaatga aagctaaatt ttatgaaggc tatattcttt attattctct
     5521 tcgcttttca aacctatctt atttatctat ccataagtat ctccgataaa aaacaaaaga
     5581 ctattgaatt aacatcattg aattgttttg tcatattatt tctaatctat gataaattaa
     5641 tttttctctt tattgcctat gtttttttga taatttttat attaaacttg ttccgtaact
     5701 gaaaagtgat atactatatg gatacaaaaa tgatgtcaca atttgcagtt atggataatg
     5761 aaatgcttgc ttgcgttgaa ggtggagata ttgattgggg aagaaaaatt agttgtgcag
     5821 caggggttgc atatggcgca attgatgggt gtgcaacaac ggtttgacat ttctattggg
     5881 accatttgct ataggaatag gtgtaactgg tgctgcggga ggagctatac ttggaggtgt
     5941 ggcctatgca gcgacatgtt ggtggtaatt atggatttta aaagttttat tattggttta
     6001 gtagttggta tatttggtcc ttatatggat gacttaatta gaaaaatatt ttcaaaatct
     6061 tccaaaaagg atacggatag tactctctaa aaaataaatt tctcccctgt atttgctagc
     6121 ttaatttaca ggggagtttc tttaatattg tttcgcctgt caaaatgata tgtgatagca
     6181 ggatgaaaag tctgcaaaca agttggtatc aatacaatat aaagaggaaa gtatgttttt
     6241 aatgcttact ttttaaattt tttaacaatt caagatgttt cgatgacaat tcaagatttg
     6301 gatgaaaaat tttaaaaaac aatgatatac taaacttgtc aaagttgcaa cgggtgctgc
     6361 tacattatgt taaaaatcgc tcttgattga taagctggat ttgttaggaa aggagtaaat
     6421 aattatggat aagaaaaaga tagtttcgac tataatatgt atagtatttc ttgtagtgtc
     6481 agtagataat ttttttagag acttaacccc gttactgttc attttaaata ttattggtct
     6541 gtcttgcttt tcagtgctaa catacattaa cataaaagag atactcttaa atataagtaa
     6601 gtgatgattt ggttagtcct agaaccaatc aacttatgtt tatcggcttg gcagatttta
     6661 tgtttgtcat ttgtttgtat agaggtattt cagaaacaga attctatcaa caactaatcg
     6721 cttatatagg tgtattctct gcttgtttat cacgcttctg ctcatgtggg gcttgaagta
     6781 ttataaaaag tagctttcta tccttttctg acaggatatt tgagtagaag gctctattaa
     6841 attgttagtg aaataaatga atggaggtat ttaatatgaa atacagatta ttttttgtta
     6901 ttttcttgag tagtgtgttg gatattcttt tagggacatt tttacaaatc tctatcgtat
     6961 ctattgggtg gcttgttctt tacagtggtt tgtttgaagc aggagttttc cttcttgcta
     7021 ataaaggggt ggcggtaaaa atcaaggaag tagatattcg aaatcgattc aaatttattt
     7081 ttggaaaaac cttatggttt caaattcttt tgctcatctt tttgataatc aaactttatc
     7141 ttggtttgga tgcgaggttg attttattct atggacatat tttcattgtc tttaatgtct
     7201 taatgtatct attatctagt agtcaggtta gccttaaaaa gaacaaactg tcttcttaat
     7261 cttacagcag tagagcctga caaggtgagg tcatagtagg aagaatttct atgaaaaagt
     7321 atcaacttct attcaaaata agtgcagtct tctcttactt atttttcgta tttggtcttt
     7381 ctcagctgac gcttatcgtc caaaattatt ggcaattttc ttcccagatt ggcaatttcg
     7441 tctggattca aaatatcttg agtttactat ttagcggagt catgatttgg attctggcta
     7501 agacaggcca tggctatctc tttcgcattc caagaaaaaa atggctttgg tattcgattt
     7561 tgacagtatt agtgctagtg ctccatatct cttttaacgt tcagatagct aaacatgttc
     7621 agtcaactgc tgaaggttgg gccgtattga tcggttatag tgggaccaac tttgctgagc
     7681 taggtatcta tgtaaccctg tttttcttga ctccactaat ggaagagttg atttataggg
     7741 gattactgca acacgccttc tttaagcatt cgcgatttgg ccttgatttg tttcttccgt
     7801 ccattttatt tgctcttcct catttttcac gcctacctag tttgttagat attttcgtct
     7861 ttgcaacatt tggaatcatc tttgccggtt tgacccgcta taccaagagt atttatccat
     7921 cctatgcggt gcatgtgatc aataatattg tagcaacctt cccgtttttg ctcacttttc
     7981 tacatagggt cttggggtaa aaaaacaaaa ggcttgcttt tcagccatag aggaggtcat
     8041 catgtataaa cacttatttt tctagattcc aaaactttag atcggttgac accctatatt
     8101 ctagtcttgg cttctgacac cattgctttt aatgtttttg tgctaacctt tgtatctgcg
     8161 gtggttttta atttcctaaa ttccatgcta gctttaatgg ctatcttctt aggagctggc
     8221 tatgtggtcg gattttggtt actaatactc aagaaaatca aagagcaaac taggaagcta
     8281 gccgcaggct gtacttgagt acggcaagga gacgttgacg tggtttgaat ttgattttcg
     8341 aagagtataa aatggtttgt tttggaaagg ctagaactaa agaatgacat gtaggaagtc
     8401 tgtttgttga ggaggataat tttatggagt tttttgataa atttcatgcc ttgtgttttg
     8461 gatttttagt actaataatt gtcattacag ttccttatac gattaaccat ggggattttt
     8521 ttcaaaatga atctgcattg attattgtaa gtcttcttgt aacctcgctg agtgttgctt
     8581 atgctagaaa gtttgaaatg atttcttttg ggatgttaag caagaaacaa cttttgcttt
     8641 tcattgcaat ctttcttcta agtgtacttg agacgctggt ttatattcat ttcttcgctg
     8701 tttcttctgg ctcaggggtc caacacttgg cggaagtcag cagaggaatt tccctgtctt
     8761 tgattttaac tacctcagtt tttggcccca tccaggagga actcattttc agaggacttc
     8821 ttcaaggtgc ggtttttgac aattcttggt tagggcttgt gctaacttcc tctctctttt
     8881 ctttcatgca tggaccttct aatgtccctt cgtttatttt ttatctactt gggggtttgt
     8941 tgctgggctt tgcttataaa aagagtcaaa acctatgggt ttctactcta gtccatatgc
     9001 tttacaacag ttggccactc ttaacttatt tataaaaatc atgaaaaggt aagtaggaga
     9061 ttgtgcttac ctttttcttt ctataatgat aacccaaacc actccagagg cttttctttg
     9121 agaatcgggt tagagtggtt gacgaatagg ccaaaaacta gtagaatagt aaggaaactt
     9181 tatacggagg aaagaaatgg atttgggtga taatgagcta acactgactc ccatacctgg
     9241 gaaaagtggc aaggcttata tgggtagcta tcctgatggg aagcgcatct ttgtaaaaat
     9301 gaacacctct ccaatcctac ctggtctagc tagagaacaa attgctccac aattattatg
     9361 gagtcgtcgt ttggcagatg ggcgtgatat gtgtgctcaa gaatggttga caggcaagat
     9421 attgaccccc tatgatatga atcgtaagca aatcgtcaat attttaaccc gtcttcatcg
     9481 ctcacgtccg ttgatgacac aattgagtcg tttgggctat gccatggaaa cacctgtaga
     9541 tttactacag tcttggcaag aaacggctcc agatgctttg cgtaaaaatc attttatcag
     9601 tgaagtgatg gctgatttac gtcagactat tccaggattt agagaggacc atgcgaccat
     9661 tgtccatgga gatgtacgac atagtaattg gattgagaca gatagtggct tgatttattt
     9721 agtagattgg gattcggttc gcttgaccga tcgcatgttt gatgtggccc atatgctctg
     9781 ccattatatt tcagaacatc agtggaagga atggttgacc tactacggtt acaagtacaa
     9841 tcaaacggta ttaagtaaat tgtattggta tggtcaattg tcttatttga gtcagatttc
     9901 caagtattat atgaaccaag atttagaaaa tgtcaatcgg gagattcatg gtttgcgtca
     9961 tttccgagac aagtatggaa agagaagatg agagttagaa atcgtaaagg ggcaacagaa
    10021 ttactagagg caaatcccca gtatgtggtc ctcaatccct tggaagccaa ggcaaaatgg
    10081 cgggacttgt ttggcaatga taatcccatt catgtggaag ttggaagtgg aaagggtgcc
    10141 tttgtttcag gtatggccaa gcaaaaccct gacatcaact atatcgggat tgatattcaa
    10201 aagtctgttt tgagctacgc tttggacaag gtgcttgaag ttggagtgcc taacatcaag
    10261 ctcttgtggg tagatggttc tgacttgact gactactttg aagacggtga gattgatcgc
    10321 ttgtatctga acttttcaga tccatggccg aaaaaacgcc atgaaaagcg tcgtttgacc
    10381 tacaagacct ttttggacac cttcaaacgt atcttgcctg aaaatggaga aattcatttc
    10441 aagacggata accgtggctt gtttgagtac agtttagtga gcttttctca atatggcatg
    10501 aaactcaatg gtgtctggct agatttgcat gccagtgatt ttgaaggcaa tgtcatgaca
    10561 gaatacgagc aaaaattctc aaacaagggg caagttatct accgagttga ggcagaattt
    10621 taagagatag cctaaaatta ggctgtacaa gtgcttttgc tttacataag ttggcaaacg
    10681 tgctatactg atagtaagaa tatgaaaagt gaggcgggga aatatcttcg cctcttgctt
    10741 atgaggaggt ggacgcaatc gcaacaatcg tagaattagt cagagaagtt gtagaacctg
    10801 tcatagaagc tccttttgaa ctcgtggata tcgagtatgg aaagattggc agtgacatga
    10861 ttctcag
//
