LOCUS       NZ_CLHL01000022         9127 bp    DNA     linear   CON 30-NOV-2024
DEFINITION  Streptococcus pneumoniae strain SMRU2163, whole genome shotgun
            sequence.
ACCESSION   NZ_CLHL01000022
VERSION     NZ_CLHL01000022.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  AUTHORS   Informatics,Pathogen.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2015) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001094205.1-RS_2024_11_30
            Annotation Date                   :: 11/30/2024 00:46:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,213
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 68 of 154
            Pseudo Genes (incomplete)         :: 85 of 154
            Pseudo Genes (internal stop)      :: 43 of 154
            Pseudo Genes (multiple problems)  :: 34 of 154
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:27:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9127
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CLHL01000022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..9127
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..9127
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9127
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1476:4127]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1477..4127
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(965..1159)
                     /locus_tag="AE723_RS09695"
                     /old_locus_tag="ERS021673_01886"
     CDS             complement(965..1159)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411016.1"
                     /locus_tag="AE723_RS09695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01886"
                     /product="hypothetical protein"
                     /protein_id="WP_050150399.1"
                     /transl_table=11
                     /translation="MEKIDYITLNEVELETISGGDDCFIGDIGWGLLKSIGGMIKPAPY
                     VPPVCIPKSSWNPAPPVPC"
     misc_feature    complement(1390..1406)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.78"
                     /tool="antismash"
     gene            complement(1477..1680)
                     /locus_tag="AE723_RS09700"
                     /old_locus_tag="ERS021673_01887"
     CDS             complement(1477..1680)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093272.1"
                     /locus_tag="AE723_RS09700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01887"
                     /product="bacteriocin class II family protein"
                     /protein_id="WP_001093272.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 4.7e-11,
                     bitscore: 33.6, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKMMSQFSVMDNEMLDRIEGGIFGVDDALFWAGLGYVAGSIVD
                     TAIDDFTNQCRKNPHQWFCVRV"
     gene            1970..2557
                     /locus_tag="AE723_RS13380"
                     /old_locus_tag="ERS021673_01888"
     CDS             1970..2557
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /locus_tag="AE723_RS13380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01888"
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_000205166.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.4e-43, bitscore:
                     138.6, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKTN
                     KEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQTK
                     KDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPSSDF
                     QTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            2484..4127
                     /locus_tag="AE723_RS12530"
                     /old_locus_tag="ERS021673_01889"
                     /pseudo=""
     CDS             2484..4127
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012235.5"
                     /locus_tag="AE723_RS12530"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01889"
                     /product="peptide cleavage/export ABC transporter"
                     /pseudo=""
                     /sec_met_domain="TIGR01193 (E-value: 1.3e-176, bitscore:
                     579.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVIT
                     YILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANS
                     IIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLLSIPIYMFIIFSFMKPFEKMN
                     HDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQTKLQSA
                     KVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIKQG
                     DKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINH"
     gene            4138..5499
                     /locus_tag="AE723_RS09720"
                     /old_locus_tag="ERS021673_01891"
     CDS             4138..5499
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /locus_tag="AE723_RS09720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01891"
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_050102763.1"
                     /transl_table=11
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKEI
                     TVTSQGEITPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTALAT
                     QLQRLEKQKEGLGILKQSLETTTDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTNTEV
                     SNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRYLIAS
                     QGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVLRTQFL
                     QTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRIPTGTEI
                     AQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQTIDQTPT
                     RTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILTHSD"
     gene            5556..5711
                     /gene="blpC"
                     /locus_tag="AE723_RS09725"
                     /old_locus_tag="ERS021673_01892"
     CDS             5556..5711
                     /codon_start=1
                     /gene="blpC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /locus_tag="AE723_RS09725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01892"
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="WP_000358814.1"
                     /transl_table=11
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDILYSLNIIKHNNTKGL
                     HHPIQL"
     gene            complement(5754..7094)
                     /locus_tag="AE723_RS09730"
                     /old_locus_tag="ERS021673_01893"
     CDS             complement(5754..7094)
                     /EC_number="2.7.13.3"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /locus_tag="AE723_RS09730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01893"
                     /product="sensor histidine kinase"
                     /protein_id="WP_050104728.1"
                     /transl_table=11
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILLA
                     VVFGMLGYMVGNVYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRGVS
                     YFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQKSLT
                     QINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKDKLHE
                     RLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIKEIYDS
                     VLKDSSEKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEIQVEGMN
                     LLDFLTVVSILCDNAIEASVEACQPHVSIAFLKNGAQETFIIENSIKEEGIDISEIFSF
                     GASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(7108..7845)
                     /locus_tag="AE723_RS09735"
                     /old_locus_tag="ERS021673_01894"
     CDS             complement(7108..7845)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /locus_tag="AE723_RS09735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01894"
                     /product="response regulator transcription factor"
                     /protein_id="WP_001219124.1"
                     /transl_table=11
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLLA
                     EVHEKGAHQLFFLDIEIRNEEMKGLEVARKIREQDPYALIVFVTTHSEFMPLSFRYQVS
                     ALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYYLET
                     STRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKLLFFP
                     NGGSCLIARYKVREVSEAINNLH"
     gene            complement(7850..8188)
                     /locus_tag="AE723_RS09740"
                     /old_locus_tag="ERS021673_01895"
     CDS             complement(7850..8188)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /locus_tag="AE723_RS09740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01895"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_000682123.1"
                     /transl_table=11
                     /translation="MKHMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEKASFNML
                     QNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYREIR
                     QKWLKEGE"
     misc_feature    complement(8312..8325)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.71"
                     /tool="antismash"
     misc_feature    8400..8418
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 23.85"
                     /tool="antismash"
     gene            8462..8791
                     /locus_tag="AE723_RS09745"
                     /old_locus_tag="ERS021673_01896"
     CDS             8462..8791
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /locus_tag="AE723_RS09745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01896"
                     /product="hypothetical protein"
                     /protein_id="WP_000132572.1"
                     /transl_table=11
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGFL
                     AKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLILNE
                     NQRAN"
ORIGIN
        1 atcaggacag tcaaatcgat ttctaacaat gttttagaag tagaggtgta ctattctagt
       61 ttcaatctac tatactatac catattttgt ttcgcaggga atctattata aaagggtaag
      121 tattgcaaaa acacttaccc ttttatactt cattaacttc tactttttat aatacttcaa
      181 cccccaaacg aaaggaagat gttccgtttt ttagcaagaa aagttttcat ttctttgctc
      241 ctttcttttt agttaaagca aaatagatca taactgcaat cacataggct atggtataaa
      301 atagctgata ccaaatactt tccctaagcg gataaagaaa gatggacatg attagataca
      361 agatgaaaat gataagtatt tttttcttca taaaatttcc tcctaaatgt gtgatttatc
      421 ttagttgaac aagaatcttt acactgctag tatagcactc attttgacct tggattactc
      481 aaatcatgaa tggtcatcaa aacatcttga acaataacaa gattttcatc tatacaaaaa
      541 tagttatact caacaacaat caaactaaag aaccaaataa atatagctta acatactttt
      601 tttggatttc aaacaatact gataaaatta ataataaatc tgtggtagta cttcccatcc
      661 ctactgcttt ccaattttat aatattatta attaaataat atatgagaaa aaacgaacaa
      721 gactgtgtta ataatctatc ttattcgttt tatttattag acttgttcag aaattatttg
      781 aagcagtatt agctagttag aaagtgaatt tttaacgttc caaccagcca ttagtttcta
      841 gttttcgact cttgcaataa gctaaaataa tcctgaacaa cacctgtata ttatagttac
      901 gaagattcaa caaaatagcg aacaagtcga ttattcattt tttatataag tttacaataa
      961 ctttttaaca tggaactgga ggtgcaggat tccacgaact ttttggaatg catactggag
     1021 gaacatatgg agcaggtttt atcatccctc cgatactctt caaaagcccc catccgatat
     1081 ctccaataaa acagtcatct ccaccactaa tcgtctctaa ttctacttca ttcaaagtaa
     1141 tataatcaat tttttccatt gttataattc attcctttcg ttttactcaa ggcacaacac
     1201 agaatgaaaa agtgttgtac tctttatttt ataatgttta taataatagt gagaaaacct
     1261 atcactacta caaatcacgg ggaggtgaat aagtgagtga tatagccaat atctcgcatt
     1321 tttataggtg tcattctttc tatcaagcac aagaactgtg actaggagca cgtaaactta
     1381 taacagaata agcgactcgt ttgcaaaaga atagtttcta tcactgcata ggtcattaag
     1441 ttatataaat tcttatacaa tacaacacta ctatatctat acacgaacac agaaccactg
     1501 atgaggattt ttccggcatt gattcgtaaa atcatcaatt gctgtatcaa caatgctacc
     1561 tgcaacatag cctaaccctg cccaaaacaa agcatcgtca actccaaata ttcctccctc
     1621 tatcctatca agcatttcat tatccataac agaaaattgt gacatcattt ttgtattcat
     1681 gacaaatact ccttttttat ttttgtcttg ttgtaacttt gataagttta gtatatcatc
     1741 gttttttaaa atttttcatc cagatcttga attgtcatcg aaacgtcttg aattagcttt
     1801 tttatttcaa gccacctcta tttcaagcca cctctaaatg tttaaaaaaa ataatttcta
     1861 atcacttttt taccattcag gaagttttaa tgactattca agatttcata aaatatgaac
     1921 ttagttttat gacataatag acctatccac tatatgaaag gaattgccaa tgacttctta
     1981 taaacgtaca tttgttcctc aaatagatgc gagagactgt ggtgtcgctg ccttagcctc
     2041 gattgctaaa ttctatggtt cagatttttc tctagctcac ttgagagaac ttgcaaagac
     2101 caataaagaa gggacgactg ctcttggcat tgtaaaagcc gctgatgaaa tgggctttga
     2161 aacaagacct gttcaagcag ataaaacgct ctttgacatg agtgatgtcc cctatccatt
     2221 tatcgttcac gttaacaaag aaggaaaact ccaacattac tatgttgtct atcaaacaaa
     2281 gaaagactat ctgattattg gtgatcctga cccttctgta aaaatcacta aaatgtcaaa
     2341 agaacgcttt ttctatgaat ggactggagt agctattttt ctagctacca aacccagcta
     2401 tcaaccccat aaagataaaa agaatggtct actaagcaag cttccttcct ctgattttca
     2461 aacaaaaatc tctcattgct tacattgttc tctcaagctt attggtcact attatcaata
     2521 taggtggttc ttactatctc caaggaatct tggatgaata cattccaaat cagatgaaat
     2581 caactttagg aatcatctca gttggtctgg ttatcaccta tatcctccaa caagtcatga
     2641 gcttctccag agattatctc ctaaccgttc tgagccagag attaagcatt gatgtgattt
     2701 tatcctatat tcgccatatt tttgaacttc ccatgtcttt ctttgcgaca cgtcgtacag
     2761 gagaaatcat ttcacggttc acagatgcta actctattat agatgccttg gcttctacca
     2821 ttctttctct ttttctggat gtttctattc tgattcttgt agggggagtc ttgctggcac
     2881 aaaaccctaa tctcttcctc ctttctcttc tttccattcc tatatacatg ttcatcatct
     2941 tttcttttat gaaacctttt gaaaaaatga accatgatgt catgcaaagt aattctatgg
     3001 ttagctctgc cattatcgaa gatatcaacg ggattgaaac tataaagtcg ctcacgagtg
     3061 aagaaaatcg ctatcaaaat atagacagcg aatttgtaga ttatttggaa aaatccttta
     3121 agctcagtaa atattctatt ttacaaacga gtttaaagca gggaacaaaa ttagttctga
     3181 atatccttat cctatggttt ggcgctcaat tagtcatgtc gagtaaaatt tctatcggtc
     3241 agctgattac ctttaacaca cttttttctt actttacaac tcctatggaa aatattatca
     3301 acctccaaac caaactccaa tctgcgaagg tcgctaataa ccgtttgaac gaagtctatc
     3361 tagtcgaatc tgaatttcaa gttcaagaaa accctgttca ttcacatttt ttgatgggcg
     3421 atattgaatt tgatgacctt tcttataagt atggttttgg acgagatacc ttaacagata
     3481 ttaatctcac gattaaacaa ggagataagg ttagcctagt tggagttagt ggttctggta
     3541 aaacaacttt agccaaaatg attgtcaatt tctttgaacc ctacaaaggg catatttcca
     3601 tcaatcatta ggatattaaa aacattgata aaaaagtctt gcgccgtcat attaattacc
     3661 taccccaaca agcctatatc tttaatggct ctatcttgga aaacttaacc ttgggcggta
     3721 atcatatgat tagccaagaa gatattctaa gagcttgtga attagctgaa attcgtcaag
     3781 acattgaaag aatgcctatg ggctatcaaa ctcagctctc tgatggagct ggtctatcag
     3841 gaggacaaaa gcaacgaata gccctcgctc gtgctctttt aactaaatct cctgttttaa
     3901 tactagacga agctactagc ggtcttgatg tcttgactga gaaaaaggtt atagataatc
     3961 ttatgtccct aactgataaa accattctct ttgtagccca tcgtctcagt atagccgaac
     4021 gaactaaccg tgtcattgtt cttgaccagg ggaaaatcat tgaagttggt agtcaccaag
     4081 agttaatgca agcgcaagga ttctaccatc atctattcaa taaataagga gaatgtcatg
     4141 aatcctaatc tttttagaag cgtcgagttt tatcagagac gttaccataa ctatgcgaca
     4201 gtgttaatta tacctctttc attactattt actttcatct tgattttctc ccttgttgcc
     4261 acaaaagaaa ttactgttac ttcccaaggg gaaatcaccc ctacaagtgt cattgcatct
     4321 attcagtcga ccagtgataa tcctatcctt gctaatcatt tagtggcaaa tcaagtagtt
     4381 gaaaaagggg acttactcat caaatactct gaaacaatgg aagaaagtca gaaaactgcc
     4441 ttagcaactc aattacaaag acttgagaag caaaaagaag gacttggaat tttgaaacaa
     4501 agcttagaaa caacgactga tcttttttct ggcgaggatg aatttggcta ccataatacc
     4561 tttatgaatt ttactaaaca atcccatgat attgaactgg gtatcacaaa gactaacact
     4621 gaagtttcaa atcaagctaa tctttccaat agcagttcat cagctattga acaagaaatt
     4681 acaaaagttc aacaacaaat tggagaatat caagagttga gagatgctat cataaataac
     4741 agagcacgct taccaactgg caatccgcac cagtcaattt taaatcgtta tcttatagcc
     4801 tctcaaggac aaacacaagg aactgcagag gagccatttt tatctcaaat taatcaaagt
     4861 attgcaggtc ttgaatcatc tatcgcaagc ctcaaaattc agcaagctgg tatcggaagt
     4921 gtagcaactt atgataacag tttagcaacc aaaattgaag tactccgcac tcagttttta
     4981 cagacagcct cacagcaaca actaaccgtg gagaaccaat taacagaatt aaaagtacaa
     5041 ctagatcaag ccacacagcg tttggaaaac aataccttaa cctccccaag taaaggtatc
     5101 gttcatctga acagcgaatt tgaaggtaaa aatagaattc caactggtac agaaattgct
     5161 caaatattcc ctgtcatcac agatacaaga gaagtactaa tcacttacta cgtatcttct
     5221 gactatctac ctctactaga taaaggacaa actgtaagat taaaactgga gaagattgga
     5281 aatcacggca ccaccatcat cggccaactt cagacaattg atcaaactcc taccagaaca
     5341 gagcaaggaa atctctttaa attaaccgct cttgcaaaac tatctaacga ggatagtaaa
     5401 ctcatccaat atggcttaca aggtcgcgtc actagtgtaa ctacaaagaa aacatatttt
     5461 gattatttca aagataaaat tttaacacat tctgattaat tttcagataa cactctataa
     5521 ctatttatta tcttatcaaa aaggagaatc ataacatgga taagaaacaa aacctaactt
     5581 catttcaaga actaacaact accgaactca accaaattac aggtggagga ttgtgggaag
     5641 atatcttata cagccttaat ataattaagc ataacaatac gaaaggactt catcatccaa
     5701 tacaactata aaaaataaga ccgagaaaca agtactctcg gtcttatttt tcatcattct
     5761 gtatgtatca cagtaagtac ctgacgaaag acttgatttt gacaggtggt atttagactg
     5821 gtattaggat ggctttccac aatcttcatg acggtataga gaccaactcc tctctcctcc
     5881 cctttagaac ttgctccaaa ggagaagatt tcagaaatat cgatgccctc ttctttgatg
     5941 gagttttcaa tgataaaagt ctcctgtgct ccatttttta aaaaggcaat tgaaacatga
     6001 ggttgacagg cttctacact agcttcaata gcgttgtcac aaaggataga cacaacggtt
     6061 agaaaatcaa gtagattcat cccctctacc tgaatctcct ctggaacttc gacattaaag
     6121 acaatgttct tatctctagc ttttataaat tttccagcta ggagactttt gagggcacga
     6181 tcccgaacat tcaccaatcg gccaaggtca tatttattgt cctgcaattt ttcactagaa
     6241 tcctttaaga ccgagtcgta gatctctttt atctgctcca tatcctcctc ttcaatgccc
     6301 agacgtaagc tagtcaagag gttggtgtag tcatggcgaa aactccgtac ttccttataa
     6361 agctcctcta tatgccgact ataccgctcc atctctctgt agcgcaagtc ctgctcttgg
     6421 ttcagtctct catggagttt gtccttcaaa taggtatcca atttcttgat aatccccata
     6481 aaaaagagca ggtaaaagac taggatgaga tggcgaacag tcgttgattg aatcccctgt
     6541 tcatattcaa agtaagacag attttgtatc accaaatagt aagcccccat tatccagtta
     6601 atctgagtca gggacttttg aaaatcttta tcgagaatcc cctttctcaa gctagtgaaa
     6661 tcatagtcca accatttcaa aaaagctaga gaaatgaaga aattgaaaat tattatacat
     6721 aaccaaataa atgagtagtc atcatatact tgcccttgtc ccaaaaatgg aagcacaaaa
     6781 taggaaacac ctctataaaa gagattcact aatatcattg gaaagagacc ataaaagaga
     6841 aggagttttt taggaagttc tcttaacaat aagaaagata agcctatgcc gtacaagggt
     6901 tccataaaat aagataggta aacatttcct accatatagc caagcattcc aaaaacaacg
     6961 gccaacagta tcttaaaaag aaaggcctta aaaatcctct caaaagtgag actaattcca
     7021 tctaccttaa agaatatgac aatttttaat ccgtgagtaa caagtgtata caacaatatc
     7081 caagcaacat tcataaactc tcctagctca gtgtaagtta ttaatagcct cagacacttc
     7141 cctgacctta taacgcgcga ttagacaact tccaccattg ggaaagaaaa gcagtttttc
     7201 tttcttatct aaatgcacca cattggcagg attgatgaga aaagagcgat ggcactgcaa
     7261 gagacgaggc tcctgcttga gaacctcctc tagactcgct gtaaattcca gcctgtctgt
     7321 cttggtatag agaataacac gatggggtct tgtggacgtt tcgagatagt aaacctcttt
     7381 aaaaggatac tggaattgag caaattttga tttaaagtaa aagcaatctt ccgctagact
     7441 tttactgtct tgaccattgg catagaggag ggctgtctcg atacgagatt caaactcctc
     7501 tgccgaaagg gccttatcaa tatagtccag agcagacact tggtagcgaa aggacagggg
     7561 cataaactcc gagtgagtcg tcacaaagac aatcagggca taggggtctt gttcccgaat
     7621 ctttctagcc acttccagcc ccttcatctc ctcatttcga atctcaatgt ccaaaaagaa
     7681 tagctgatgg gcccccttct catgcacctc tgccagcagt tggtccggct tgccaaatac
     7741 ctcaaaagag ctaggaatga tatgatgtgc tttcaaaagt ttctcaatcg tcgtttcaat
     7801 tctagtctgt tgggaaaaat catcttctaa aacaaatatt ctcatcttct tactctccct
     7861 ctttcaacca tttttgacga atttctctat agcgacgtct ggcatacttg acagcgtatt
     7921 gaccttcttc tccgagaaaa aggattcttt cttgaaaatc aatcgatttt aatttatcaa
     7981 gattaaccaa acaatttcga tgacatctca tcaaggtttc cccatattgg ttctcaagat
     8041 tacttaaatt ttgaagcata ttaaaactag ctttttctgt aacaatctgt acggtatggg
     8101 ctttagttgg atgtgtttgg atatagtaga tatcgtcgat gtttacttta tagactgttt
     8161 tctgtgtctg aataatcata tgtttcattg gattctcctc ctaagaaaag aataacagaa
     8221 aaggtctagc ccaagcacac ccaatcctaa acagtctttg aaaactccta aaaggattat
     8281 tttctacaac atcatgatga gcttacaact attttatcat aaaattcact cagcgcaatt
     8341 caagacattt caatgacaat taaagatttg aataaaaaaa gaattaaaag tgataaacta
     8401 acagtatcaa aacacagttg ccagaagtaa gactggcacc cagattaaag gaggcattcc
     8461 catgacagat acagacccca tcaaaagagc tcatactttg attactgact taaacaaagc
     8521 ctaccaagca tgcaaacagg caagcgctga cgacgtccgc tttcaggagc aattaaactc
     8581 tattcttggt tttctagcca aggctgaaac agtggataat cgattcttga ttgaactaga
     8641 aaaattttac cagacttcca gtcttctcat gggactcagc gctcttgatc cagatgctcc
     8701 aactcgtgcc gcttggcggg cctatgaccg cttccacttt gaccaagtca agaccaagtt
     8761 aatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaagctgt acttgagtac
     8821 ggtaagacga cgctgacgtg gtttgaagag attttcgaag agtataagtc tctacggacc
     8881 aaccattatc ctgtagtaac aaaaaagaag ttgagacaga ctgaactcaa cttctttttt
     8941 agtgtcgtat caacaatgtt agtcctgcat ctgacgtttt acctgataag gcaaatacaa
     9001 gactagtaaa accaaaccag ctcctagtaa ggctagaagg gctagttttt cttggaaggt
     9061 aatcagccca acaactaatt ccacaagtaa aacaaaactg gtcaatcctc gaactacctc
     9121 ctgtaag
//
