LOCUS       NZ_CLHL01000009        10474 bp    DNA     linear   CON 30-NOV-2024
DEFINITION  Streptococcus pneumoniae strain SMRU2163, whole genome shotgun
            sequence.
ACCESSION   NZ_CLHL01000009
VERSION     NZ_CLHL01000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  AUTHORS   Informatics,Pathogen.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2015) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001094205.1-RS_2024_11_30
            Annotation Date                   :: 11/30/2024 00:46:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,213
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 68 of 154
            Pseudo Genes (incomplete)         :: 85 of 154
            Pseudo Genes (internal stop)      :: 43 of 154
            Pseudo Genes (multiple problems)  :: 34 of 154
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:27:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 15285
            Orig. end                         :: 25759
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CLHL01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10474
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10474
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10474
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5474](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5474)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    645..660
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.45"
                     /tool="antismash"
     gene            complement(693..1172)
                     /gene="rimP"
                     /locus_tag="AE723_RS06495"
                     /old_locus_tag="ERS021673_01268"
     CDS             complement(693..1172)
                     /GO_process="GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /gene="rimP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /locus_tag="AE723_RS06495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01268"
                     /product="ribosome maturation factor RimP"
                     /protein_id="WP_001808916.1"
                     /transl_table=11
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVDK
                     PEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGKYI
                     HVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(1298..1933)
                     /gene="trmB"
                     /locus_tag="AE723_RS06500"
                     /old_locus_tag="ERS021673_01269"
     CDS             complement(1298..1933)
                     /EC_number="2.1.1.33"
                     /GO_function="GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="trmB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /locus_tag="AE723_RS06500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01269"
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="WP_001266083.1"
                     /transl_table=11
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVEV
                     GSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTDYF
                     EDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFEYSL
                     VSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(1930..2724)
                     /gene="ccrZ"
                     /locus_tag="AE723_RS06505"
                     /old_locus_tag="ERS021673_01270"
     CDS             complement(1930..2724)
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /GO_function="GO:0004747 - ribokinase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /GO_function="GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /GO_process="GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ccrZ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /locus_tag="AE723_RS06505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01270"
                     /product="cell cycle regulator CcrZ"
                     /protein_id="WP_000363002.1"
                     /sec_met_domain="APH (E-value: 1.8e-19, bitscore: 63.1,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGLA
                     REQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQLS
                     RLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDVRHS
                     NWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTVLSKL
                     YWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(2885..3483)
                     /locus_tag="AE723_RS06510"
                     /pseudo=""
     CDS             complement(2885..3483)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /locus_tag="AE723_RS06510"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="lysostaphin resistance A-like protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFKMNLH"
     gene            complement(3513..3761)
                     /gene="blpZ"
                     /locus_tag="AE723_RS06515"
                     /old_locus_tag="ERS021673_01272"
     CDS             complement(3513..3761)
                     /codon_start=1
                     /gene="blpZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /locus_tag="AE723_RS06515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01272"
                     /product="immunity protein BlpZ"
                     /protein_id="WP_000276499.1"
                     /transl_table=11
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFNF
                     LNSMLALMAIFIGAGYVVGFWLLKWFVLERLDLKNDV"
     gene            complement(3803..4492)
                     /locus_tag="AE723_RS06520"
                     /old_locus_tag="ERS021673_01273"
     CDS             complement(3803..4492)
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /locus_tag="AE723_RS06520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01273"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000760541.1"
                     /sec_met_domain="Abi (E-value: 1.4e-11, bitscore: 37.2,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKYQLLFKTSAILSYLFFVFGLSQLTLIVQNYWQFSSQIGNLFW
                     IQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKHVQ
                     STAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDLLLP
                     SILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFPFLLT
                     FLHRVLG"
     gene            complement(4524..4757)
                     /locus_tag="AE723_RS06525"
                     /old_locus_tag="ERS021673_01274"
     CDS             complement(4524..4757)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379963.1"
                     /locus_tag="AE723_RS06525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01274"
                     /product="hypothetical protein"
                     /protein_id="WP_050150746.1"
                     /transl_table=11
                     /translation="MDTKMMSQFSVMDNEMLVRTEDGDVSDIYRGYANQRSPFASYPSI
                     LKNSGPFPVSGYCPRGYRDLGYIGAGFHLCGI"
     gene            complement(5001..5204)
                     /gene="blpN"
                     /locus_tag="AE723_RS06530"
                     /old_locus_tag="ERS021673_01275"
     CDS             complement(5001..5204)
                     /codon_start=1
                     /gene="blpN"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /locus_tag="AE723_RS06530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01275"
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="WP_001099492.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.4e-13,
                     bitscore: 43.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGPW
                     GAAIGGIGGAVVCGYLAYTATS"
     gene            complement(5220..5474)
                     /gene="blpM"
                     /locus_tag="AE723_RS06535"
                     /old_locus_tag="ERS021673_01276"
     CDS             complement(5220..5474)
                     /codon_start=1
                     /gene="blpM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /locus_tag="AE723_RS06535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01276"
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="WP_000379877.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 5.1e-20,
                     bitscore: 64.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGLG
                     TAICAASGVGAPFMGACGYIGAKFGVALWAGVTGATGGF"
     gene            complement(<5627..6448)
                     /locus_tag="AE723_RS13040"
                     /pseudo=""
     CDS             complement(<5627..6448)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /locus_tag="AE723_RS13040"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS5 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDPYQGVNRLPAKQNYEASKQLTDARFKRLVGVQRTTFEEMLAVL
                     KTAYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLIRRSQWVEV
                     TLVQSGVTISRTPLSSEDTVMIDATEVKINHPKKTISESFW"
     gene            complement(<6431..6586)
                     /locus_tag="AE723_RS13240"
                     /pseudo=""
     CDS             complement(<6431..6586)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016232.5"
                     /locus_tag="AE723_RS13240"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDEK
                     NIDPYQG"
     misc_feature    complement(6921..6937)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 24.66"
                     /tool="antismash"
     gene            6937..8487
                     /locus_tag="AE723_RS06550"
                     /old_locus_tag="ERS021673_01279"
     CDS             6937..8487
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753909.1"
                     /locus_tag="AE723_RS06550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01279"
                     /product="leucine-rich repeat protein"
                     /protein_id="WP_050150771.1"
                     /transl_table=11
                     /translation="MLSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNIK
                     YKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFSYY
                     PDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSELEE
                     IELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSVKTLG
                     SNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKETKELA
                     SYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGNLELKEL
                     ILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNKSTEFSVK
                     KDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPKKNSNQGVV
                     GWVKDKGLWYYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
     gene            complement(8967..9116)
                     /gene="rpmG"
                     /locus_tag="AE723_RS06555"
                     /old_locus_tag="ERS021673_01280"
     CDS             complement(8967..9116)
                     /GO_component="GO:0005840 - ribosome [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /locus_tag="AE723_RS06555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01280"
                     /product="50S ribosomal protein L33"
                     /protein_id="WP_001265622.1"
                     /transl_table=11
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVVF
                     TEVK"
     gene            complement(9132..9314)
                     /gene="rpmF"
                     /locus_tag="AE723_RS06560"
                     /old_locus_tag="ERS021673_01281"
     CDS             complement(9132..9314)
                     /GO_component="GO:0015934 - large ribosomal subunit
                     [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /locus_tag="AE723_RS06560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01281"
                     /product="50S ribosomal protein L32"
                     /protein_id="WP_000290417.1"
                     /transl_table=11
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSLK
                     GYYKGRKIAKAASAE"
     gene            complement(9466..9666)
                     /locus_tag="AE723_RS06565"
                     /old_locus_tag="ERS021673_01282"
     CDS             complement(9466..9666)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /locus_tag="AE723_RS06565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_01282"
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="WP_000796544.1"
                     /transl_table=11
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECVK
                     QRKKDGKYVSETIDEPFEIDI"
     misc_feature    complement(10052..10069)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.23"
                     /tool="antismash"
ORIGIN
        1 cataaacttc tggaatttct tgctccatca aacgtttgat catttctgga tggctacggc
       61 taacaaagac gttcacacca cgagggttgt cttcaacctt gtaaacataa acttcgatac
      121 gatcatgaga agcaaaaact tctccaggaa tttggtcttg ttttgacaat tgggcttcga
      181 tgctaccaag gttgacatag ataaagcggt tgtcaaagcg ttctactgta ccagacatga
      241 tttcttgctc atgttctttg taagtattgt aagtgatggc acgtgtttgc ttgcgcattt
      301 tttccatgat ggtttgtttg gcagattggg ctgctacacg accaaactca gctggtgctt
      361 cttcaaactt gattttgtct ccaagttcat aagctgaatt aatggcaaga gcatctttca
      421 agctgatttc caaacggcta tcaaatactt catcaacaac ttcacggaca gtataaactg
      481 taaagtcacc tgttttttcg ttgaagtcaa tagctacgct gtctgactga ccatagcgtc
      541 tgcgataagc ggaacgaagc gactctacta ctgcgtcgat gatatcttct tttttgattc
      601 ccttgtcttc ttccaaaatg cggaaggcct ctagcatttc tttactcatg ttttcttcac
      661 ttttgaatcc tcaaaagcta tcctttcttt ttctataatt taactgctaa acgtgctttt
      721 gatactaaac tgtatggaat ttggacggtt ttcttacgcg tcttgtccat atattccata
      781 gtcaactcgt cctcttcgaa ggccaacaag gttccttcaa agaccttttg cttatcgatg
      841 gcttggtaga gcccgacatg gatgtatttt ccaaccgctc cagcgacggc atccttggtt
      901 ttcaaaggac gttccaagcc tggactggta atttctagga aatattgttc tgggaaggga
      961 tctggcttga tggtgtctag gacaggactg atcatttctg ttaagtctgc cgtgtcgttc
     1021 aaggtaattc cttcgggttt atctacaaaa atactgagaa tcatgtcact gccaatcttt
     1081 ccatactcga tatccacgag ttcaaaagga gcttctatga caggttctac aacttctctg
     1141 actaattcta cgattgttgc gattgcgtcc acctcctcat aagcaagagg cgaagatatt
     1201 tccccgcctc acttttcata ttcttactat cagtatagca cgtttgccaa cttatgtaaa
     1261 gcaaaagcac ttgtacagcc taattttagg ctatctctta aaattctgcc tcaactcggt
     1321 agataacttg ccccttgttt gagaattttt gctcgtattc tgtcatgaca ttgccttcaa
     1381 aatcactggc atgcaaatct agccagacac cattgagttt catgccatat tgagaaaagc
     1441 tcactaaact gtactcaaac aagccacggt tatccgtctt gaaatgaatt tctccatttt
     1501 caggcaagat acgtttgaag gtgtccaaaa aggtcttgta ggtcaaacga cgcttttcat
     1561 ggcgtttttt cggccatgga tctgaaaagt tcagatacaa gcgatcaatc tcaccgtctt
     1621 caaagtagtc agtcaagtca gaaccatcta cccacaagag cttgatgtta ggcactccaa
     1681 cttcaagcac cttgtccaaa gcgtagctca aaacagactt ttgaatatca atcccgatat
     1741 agttgatgtc agggttttgc ttggccatac ctgaaacaaa ggcacccttt ccacttccaa
     1801 cttccacatg aatgggatta tcattgccaa acaagtcccg ccattttgcc ttggcttcca
     1861 agggattgag gaccacatac tggggatttg cctctagtaa ttctgttgcc cctttacgat
     1921 ttctaactct catcttctct ttccatactt gtctcggaaa tgacgcaaac catgaatctc
     1981 ccgattgaca ttttctaaat cttggttcat ataatacttg gaaatctgac tcaaataaga
     2041 caattgacca taccaataca atttacttaa taccgtttga ttgtacttgt aaccgtagta
     2101 ggtcaaccat tccttccact gatgttctga aatataatgg cagagcatat gggccacatc
     2161 aaacatgcga tcggtcaagc gaaccgaatc ccaatctact aaataaatca agccactatc
     2221 tgtctcaatc caattactat gtcgtacatc tccatggaca atggtcgcat ggtcctctct
     2281 aaatcctgga atagtctgac gtaaatcagc catcacttca ctgataaaat gatttttacg
     2341 caaagcatct ggagccgttt cttgccaaga ctgtagtaaa tctacaggtg tttccatggc
     2401 atagcccaaa cgactcaatt gtgtcatcaa cggacgtgag cgatgaagac gggttaaaat
     2461 attgacgatt tgcttacgat tcatatcata gggggtcaat atcttgcctg tcaaccattc
     2521 ttgagcacac atatcacgcc catctgccaa acgacgactc cataataatt gtggagcaat
     2581 ttgttctcta gctagaccag gtaggattgg agaggtgttc atttttacaa agatgcgctt
     2641 cccatcagga tagctaccca tataagcctt gccacttttc ccaggtatgg gagtcagtgt
     2701 tagctcatta tcacccaaat ccatttcttt cctccgtata aagtttcctt actattctac
     2761 tagtttttgg cctattcgtc aaccactcta acccgattct caaagaaaag cctctggatt
     2821 ggcttgggta tcattatagc ttgaaaaagg taagcaccgt cttctgctta ccttttcata
     2881 atttttataa ataatatagg agtggccaac tgttgtaaaa catgtggact agagtagaaa
     2941 cccataggtt ttggctcttt ttataagcaa ggcccagcaa caagccccca agtagataaa
     3001 aaataaacga agggacatta gaaggtccat gcatgaaaga aaagagagag gaagttagca
     3061 caagccctaa ccaagaattg tcaaaaaccg caccttgaag aagtcctctg aaaatgagtt
     3121 cctcctggat ggggccaaaa actgaggtag tcaaaatcaa agacagggaa attcctctgc
     3181 tgacttccgc caagtgttgg acccctgagc cagaagaaac agcgaagaaa tgaatataaa
     3241 ccagcgtctc aagtacactt agaagaaaga ttgcaatgaa aagcaaaagt tgtttcttgc
     3301 ttaacatccc aaaagaaatc atttcaaact ttctagcata agcaacactc agcgaggtta
     3361 caagaatcaa tgcagattca ttttgaaaaa acccccatgg ttaatcgtat aaggaactgt
     3421 aatgacaatt attagtacta aaaatccaaa acacaaggca tgaaatttat caaaaaactc
     3481 cataaaatta tcctcctcaa caaacagact tcctacacgt cattctttag atctagcctt
     3541 tccaaaacaa accattttag caaccaaaat ccgaccacat agccagcccc tatgaatata
     3601 gccattaaag ctagcatgga atttaggaaa ttaaaaacca ccgcagatac aaaggttagc
     3661 acaaaaacat taaaagcaat ggtgtcagaa gccaagacta gtatataggg tgtcaaccga
     3721 tctaaagttt tggaatctag gaaaaataag tgtttataca tgatgacctc ctctatggct
     3781 gaaaagcaag ccttttgttt ttttacccca agaccctatg tagaaaagtg agcaaaaacg
     3841 ggaaggtcgc tacaatatta ttgatcacat gcaccgcata ggatggataa atgctcttgg
     3901 tatagcgggt caaaccagca aagatgattc caactgttgc aaagacgaag atatctaaca
     3961 gactaggcag gcttgaaaaa tgagggagag caaataaaat agaaggaaga agcaaatcaa
     4021 gaccaaatcg cgaatgctta aagaaagcat gttgcagtaa tcctctataa atcaattctt
     4081 ccatcagtgg aaccagaaag aacagggcta tataaatacc tagctctgca aagttagtcc
     4141 cactataacc aatcaataca gcccaacctt ccgcagttga ctgaacatgt ttagctgtct
     4201 gaacgttaaa agagatctgg aacactacca ctaatactgt caaaatcgaa taccaaagcc
     4261 atttttttct tggaatgcgg aagagataac catggcctgt cttaacaaga accacaatca
     4321 tgactccaat aaaaagtaaa ctcaagatat tttgaatcca gaataaattg cctatctgag
     4381 aagaaaattg ccaatagttt tggacgataa gcgtcagctg agaaagacca aatacgaaaa
     4441 ataggtagga taaaattgca cttgttttga atagaagctg atactttttc atagaaattc
     4501 ttcctcattt cttatatttc agcctatatt ccacataaat gaaaacctgc tcctatataa
     4561 ccaaggtcac gataaccacg cgggcagtac ccacttactg ggaaaggacc tgaatttttt
     4621 aatatagaag ggtaagaagc gaatggactt ctctgattag cgtaccctct ataaatatca
     4681 gagacatccc catcctctgt acgaacaagc atttcattat ccataacaga aaattgtgac
     4741 atcatttttg tatccatata gtatatcact tttcagttac ggaacaagtt taatataaaa
     4801 attatcaaaa aaacataagc aataaagaga aaaattaatt tatcatagat tagaaataat
     4861 atgacaaaac aattcaatga tgttaattca atagtctttt gttttttatc ggagatactt
     4921 atggatagat aaataagata ggtttgaaaa gcgaagagaa taataaagaa tatagccttc
     4981 ataaaattta gctttcattt ttatgatgta gcggtatagg ctaaatatcc acaaaccact
     5041 gctcctccaa ttcctcctat tgcagcgccc catggtccta gaagtctccc atatttcact
     5101 ccacccgctg cacaacctaa agcagcaact acagctgctc ctccggaatt acctccataa
     5161 acctcactca gcattgtttc atttatatta caataagtat tcatacaagt ctccttttgt
     5221 taaaatccac ccgttgcccc tgttactcct gcccaaagag ccacaccaaa tttagctcct
     5281 atgtatccac atgctcccat aaatggcgct ccaacaccac tcgcagcaca aatagctgtc
     5341 cctaaccccc agccaccaaa agcagcacca ccaccttcta agacattagt ttgccaatta
     5401 ttcttgcctc cttcaatact agataacata gttatatcca tttcatgaaa ttgttccata
     5461 atttttgtat ccatgacaaa tactcttttt tatttttaat ttttgtcttg ttgtaacttt
     5521 gacaagttta gtatatcatc gttttttaaa atttttcatc cagatcttga atagtcatcg
     5581 aaacgtcttg aattgcaaaa attacattag acttcttgcg aaaccaaaat cctagttcat
     5641 gattgataat accagcaatc aaattcattc gtaatccgaa gcgtttacga tgatttcgat
     5701 aggttgttga aaacatttta aacgttttta ctttggcaag gatgttctca accttgcttc
     5761 tatccttaga tagcgcatgg ttataggctt tatcttcagc tattagcggc ttgagtttgc
     5821 tggatttacg tggagtttgt gcttgaggat atatcttcat gagcccttga taaccactgt
     5881 cagccaagat tttaccagct tgtccgatat ttctgcgact cattttgaac aacttcatat
     5941 catgggaata gaaacaattc tcccttgact tgtgacaatc gcctgagcct tcatagcgtg
     6001 aaatttcttt ttaccagaat gattcgctaa ttgttttttt agggtgattg atttttactt
     6061 ccgtcgcatc aatcattacc gtgtcctcag agctgagagg agttcttgaa atcgtaacac
     6121 cactttgaac aagagttact tcaacccatt ggctccgacg gattaagttg ctttcgtgga
     6181 taccaaaatc agccgcaatt tcttcataag tgcgatattc tcgcacatat tgaagagtgg
     6241 ccataagaag gtcttctagg cttaatttag gttttcgtcc accttttgcg tgtttaagtt
     6301 gataagctgt ttttaataca gctaacatct cttcaaaagt ggtacgctga acaccaacaa
     6361 gacgcttaaa tcgtgcatca gttagttgtt tacttgcttc ataattttgt ttcgcaggaa
     6421 gtctattgac tccttggtag gggtcaatgt ttttttcatc tatcccgaga attattttcc
     6481 cgccatttgt atttgcaaat gctgagtagg tttcccagaa agactctgga agattgtttt
     6541 tagctttttt gtattctaaa tcaacccctt caaattttaa gtccatattt ttcctttaca
     6601 tctgtttttt gtggttctgg tatttgttca agttgagtga taatatagcg aattgaattt
     6661 cgagagtttt tactcagtta atttcttttt taacccactt taatttcttt tttaatccgg
     6721 gttaaaaaag aaattaaagt gggttaattt ttcttgactt aaaatttaaa aatgatatac
     6781 tgtgtttatg gagagatgac aatttataaa atgtactact ctattctact agataatgat
     6841 tgaataaaac tttttataac aacggctagc aaagttataa ttttatatct attagaaaat
     6901 aaatatgtaa ggaaatttaa atgaaaaaaa actacaatat tatcattaac tacagctgcg
     6961 gttattttag cagcatatgt ccctaatgaa ccaatcctag cagatactcc tagttcggaa
     7021 gtaatcaaag agactaaagt tggaagtatt attcaacaaa ataatatcaa atataaggtt
     7081 ctaactgtag aaggtaacat aggaactgtt caagtgggta atggagttac tcctgtagag
     7141 tttgaagctg gtcaagatgg aaaaccattc acgattccta caaaaatcac agtaggtgat
     7201 aaagtattta ccgttactga agtagctagt caagctttta gttattatcc agatgaaaca
     7261 ggtagaattg tctactatcc tagctctatt actatcccat caagcataaa aaaaatacaa
     7321 aaaaaaggct tccatggaag taaagctaaa actattattt ttgacaaagg cagtcagctg
     7381 gagaaaattg aagatagagc ttttgatttt tctgaattag aagagattga attgcctgca
     7441 tctctagaat atattggaac aagtgcattt tcttttagtc aaaaattgaa aaagctaacc
     7501 ttttcctcaa gttcaaaatt agaattaata tcacatgagg cttttgctaa tttatcaaat
     7561 ttagagaaac taacattacc aaaatcggtt aaaacattag gaagtaatct atttagactc
     7621 actactagct taaaacatgt tgatgttgaa gaaggaaatg aatcgtttgc ctcagttgat
     7681 ggtgttttgt tttcaaaaga taaaacccaa ttaatttatt atccaagtca aaaaaatgac
     7741 gaaagttata aaacgcctaa ggagacaaaa gaacttgcat catattcgtt taataaaaat
     7801 tcttacttga aaaaactcga attgaatgaa ggtttagaaa aaatcggtac ttttgcattt
     7861 gcagatgcga ttaaacttga agaaattagc ttaccaaata gtttagaaac tattgaacgt
     7921 ttagcctttt acggtaattt agaattaaaa gaacttatat taccagataa tgttaaaaat
     7981 tttggtaaac acgttatgaa cggtttacca aaattaaaaa gtttaacaat tggtaataat
     8041 atcaactcat tgccgtcctt cttcctaagt ggcgtcttag attcattaaa ggaaattcat
     8101 attaagaata aaagtacaga gttttctgtg aaaaaagata catttgcaat tcctgaaact
     8161 gttaagttct atgtaacatc agaacatata aaagatgttc ttaaatcaaa tttatctact
     8221 agtaatgata tcattgttga aaaagtagat aatataaaac aagaaactga tgtagctaaa
     8281 cctaaaaaga attctaatca gggagtagtt ggttgggtta aagacaaagg tttatggtat
     8341 tacttaaacg aatcaggttc aatggctact ggttggttta cagtttctgg taaatggtac
     8401 tatacctata attcaggaga tttattagta aacacgacta cacccgatgg ctatcgagtc
     8461 aatgctaacg gtgagtgggt aggataggta tccattagct aaatctaatc attctgaact
     8521 aaaaaatctt cttacatatg taggaagatt ttttggattc tcaaaactag tttaaaagac
     8581 tacgctattt aaaccaattt taagaatgga tatataagga tattcagaaa tctagttggt
     8641 caacttttgt attttgaata taccttagat ggtgacgatg tcttggtata tctggtactc
     8701 acttacatat tctaacttat tttaactaag atacaactgt agttggtgaa tttgctacaa
     8761 atagtgtaga ggcgttattt gaaaaatata caaaaaagga accccaactt acatttgagt
     8821 tcctcttgct tttaattgtc cgaatccgta ttcttatttc aaaaaaggta gtcattcgaa
     8881 tgatttccct tgcaatatag tgaaattaac aaaaagaaaa aacgctgatc aagcgttttt
     8941 cttctgtatt gattcgtatt gaatgcttac ttcacttctg taaacacaac gtgtttgcga
     9001 agttttggtg agtatttctt caattgaagg cggtctggag tgttacgttt gtttttagaa
     9061 gtaaggtaca agcgttcacc agattctttg tgttcaagtg taatatttac gcgcatggta
     9121 tctcccttct attattcagc tgatgcagct ttagcgattt tacgtccttt gtagtatcct
     9181 ttaagtgata cacggtgaga acgtgagtaa tctccagtag tttcgtcaaa gtttacagat
     9241 ggagctgtta ctttgtagtg tgtacgacgt ttgtttttct tcgcttttga agtgcgacgt
     9301 gcaggtactg ccattttgat ttctccttta ggtatttata ttcgattcaa tctactgatt
     9361 ttcatcaacc atactaggat aacacatttt ttttgtaaag taaagttact tgacaaaaaa
     9421 agatgaaaaa attctcctgt ccgacttgtc agacaggaga agatgttaaa tatcaatctc
     9481 aaatggttcg tcaatggttt ctgatacgta ttttccgtct ttcttccgtt gcttgacaca
     9541 ctctgtgagg agatattcga tttgcccatt gactgaacga aagtcgtctt ctgcccatga
     9601 tgcgagtgca gcgtataact ttgttgagag tcgaaggggg atctgctttt ttttagcttc
     9661 agccatcttt agtaaagact tcctgtgttg acaattggtt gtgcatcatg attgccacaa
     9721 agaacgacaa ggagatttga aaccatggca gcttttcgtt cttcgtcaag ttctaccaat
     9781 tccccttcat tgagccgttc tagtgccatt tcaaccattc ctacagcacc atctacaatc
     9841 atcttccgtg catcaataat ggcagatgct tgttggcgtt gaagcataac ggcagcaatt
     9901 tctggagcat aagctaggta agtgatacgt gcttcaagga tttccaagcc agcatcctca
     9961 acacgacttt ggatttcttc acgaatacgg ttagcaacaa tttcgctaga gccacggaga
    10021 ctaccttcat ctgcttgccc gtcacccgta gtatccacat taggagacac atcgtaagga
    10081 tagatgcgga caatattacg gagggcgcta tcacattgca atgaaagata ttctttgtag
    10141 ttatcaacgt tgaagactgc cttagcggta tcgacaactc tccaagttac cgcgataccg
    10201 atttctacag ggtttcctaa gcaatcattg attttttgac gagaattgct caaggtcatg
    10261 actttgaggg aaatgtgttt cttgccaatt tcaagattta catcattgtc atttgatgat
    10321 ttagcaccta aaaaaggaga ttttgtgcta acatcaccac tttgtccaag tcgagtgtgg
    10381 tttgcagggt tgactgctac gctgaaggga ttgacaaagt aaaaaccagg ttctttgatg
    10441 gtacctgtat aattaccaaa gagtgtcaga acca
//
