LOCUS       NZ_CLHL01000005        10288 bp    DNA     linear   CON 30-NOV-2024
DEFINITION  Streptococcus pneumoniae strain SMRU2163, whole genome shotgun
            sequence.
ACCESSION   NZ_CLHL01000005
VERSION     NZ_CLHL01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  AUTHORS   Informatics,Pathogen.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2015) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001094205.1-RS_2024_11_30
            Annotation Date                   :: 11/30/2024 00:46:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,213
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 68 of 154
            Pseudo Genes (incomplete)         :: 85 of 154
            Pseudo Genes (internal stop)      :: 43 of 154
            Pseudo Genes (multiple problems)  :: 34 of 154
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:27:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 14932
            Orig. end                         :: 25220
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CLHL01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10288
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10288
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10288
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5288](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5288
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(24..40)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.51"
                     /tool="antismash"
     gene            complement(408..1280)
                     /gene="sdaAA"
                     /locus_tag="AE723_RS03895"
                     /old_locus_tag="ERS021673_00748"
     CDS             complement(408..1280)
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /locus_tag="AE723_RS03895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_00748"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="WP_000500038.1"
                     /transl_table=11
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLMER
                     NLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNEHN
                     AKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASISGA
                     EGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEVPCVK
                     RNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLATTPTGR
                     RLQKEIFGE"
     misc_feature    1264..1280
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.79"
                     /tool="antismash"
     gene            complement(1289..1960)
                     /gene="sdaAB"
                     /locus_tag="AE723_RS03900"
                     /old_locus_tag="ERS021673_00749"
     CDS             complement(1289..1960)
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /locus_tag="AE723_RS03900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_00749"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="WP_000838912.1"
                     /transl_table=11
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPTE
                     VEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKDSN
                     APHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDIPGM
                     IALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHNVNFF
                     K"
     misc_feature    2116..2132
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.23"
                     /tool="antismash"
     gene            2202..2789
                     /locus_tag="AE723_RS03905"
                     /old_locus_tag="ERS021673_00750"
     CDS             2202..2789
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /locus_tag="AE723_RS03905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_00750"
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="WP_000836450.1"
                     /transl_table=11
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLSE
                     IAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETVSA
                     PVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYS
                     AENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(2841..3704)
                     /locus_tag="AE723_RS03910"
                     /old_locus_tag="ERS021673_00751"
     CDS             complement(2841..3704)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268144.1"
                     /locus_tag="AE723_RS03910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_00751"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_001268178.1"
                     /transl_table=11
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGNVISRTSLSKIESGKATPKY
                     ENMEFLLRQINMSFEEFDYICHLYQPSQRTEIMQTYLNMTSIIGSNSLVHFFETCQDYL
                     KTHHDLPIEEIRDMLEIVIHIRQHGTEQLSNQVKQTVTKLWEKIEKQDTWYENDLKILN
                     TILFSFPIDHLHLITGKILQRLEVYKNYQHLYDLRMAILLNLSTIYLYHQDKNMCRQIC
                     YTLLEDAKKKKHYDRLAICYVRIGICRDDARLIQKGFSLLELTEETSMLSHLKKEVEIY
                     SQPKEI"
     gene            4027..4614
                     /locus_tag="AE723_RS03915"
                     /old_locus_tag="ERS021673_00752"
     CDS             4027..4614
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108787.1"
                     /locus_tag="AE723_RS03915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_00752"
                     /product="hypothetical protein"
                     /protein_id="WP_001108797.1"
                     /transl_table=11
                     /translation="MNYNLKYLLSGIVVLVFIGFLVWMRYFNQRKEEEHSDVSFEARLD
                     SEVKTLYKQLGLGEEPHYFLAYRYLHPWFDLAILPPTVEIFLTKIALVMVTPDELLIRN
                     LGNGLTFTSEHHRDLRQGLIRIPKSEMKEFEIRNWKKFFVFGDFLTIKTSQHSYYLQVR
                     DDGLQKGSLSTKHFSDLKSQDFLGLLTDKRTF"
     misc_feature    complement(4667..4680)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.42"
                     /tool="antismash"
     misc_feature    complement(4939..4955)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.57"
                     /tool="antismash"
     gene            5001..5288
                     /locus_tag="AE723_RS03920"
                     /old_locus_tag="ERS021673_00753"
     CDS             5001..5288
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /locus_tag="AE723_RS03920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_00753"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_001230165.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.1e-31,
                     bitscore: 102.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFGY
                     SDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            5340..7448
                     /locus_tag="AE723_RS03925"
                     /old_locus_tag="ERS021673_00754"
     CDS             5340..7448
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /locus_tag="AE723_RS03925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_00754"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000724257.1"
                     /transl_table=11
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDLY
                     LKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLDFT
                     TDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEMDKD
                     RIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQPMMEM
                     KRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPMLWLSHF
                     LLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTALLIGVGR
                     SLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFYQDLVEKT
                     QAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTGIKKVILMP
                     ASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFPYSDKRKENL
                     SNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKLSDGNYLKFSS
                     IQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRDIITKKFMGWKL
                     VDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVLGLASRMEKRSLV
                     ELLKGGIL"
     gene            7445..8086
                     /locus_tag="AE723_RS03930"
                     /old_locus_tag="ERS021673_00755"
     CDS             7445..8086
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /locus_tag="AE723_RS03930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_00755"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000571323.1"
                     /transl_table=11
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGALVAIVGKSGSGKSTLLN
                     LLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLMLAL
                     KYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLILADE
                     PTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     misc_feature    complement(8193..8207)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.38"
                     /tool="antismash"
     gene            8293..9099
                     /locus_tag="AE723_RS03935"
                     /old_locus_tag="ERS021673_00756"
     CDS             8293..9099
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /locus_tag="AE723_RS03935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS021673_00756"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_001038474.1"
                     /transl_table=11
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVALN
                     PDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKADLA
                     ISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQETM
                     AKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNFEKEQ
                     DDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
ORIGIN
        1 atgccatcaa agaaatgatg gatttccaag ttttttgcca tgtcttgagc agtagatgta
       61 ttctttgtcg tggtgatgta gagtggataa ctgcttgata actcctcaag caagtctata
      121 atctgaggaa agagttgagc ttcatagatg ccttttgcct tatagtaaga acgatatatc
      181 tgcatggctt cagaaatttg gtctttggac aggcaggtcg caaaactact ttcgagaggt
      241 ggtcccataa aaccacgaat agttttggca tcagggctag gcacccccag ctctttaaag
      301 gtataggtaa aggcattgtg aatcccgata gaactatcaa cgagggttcc atccaaatcg
      361 aaaaaaatcg ctgtgataga ggtcatggtt tctcctattt gataagctta ttctccgaaa
      421 atttcttttt ggaggcgacg accagtaggg gtggtagcga gtccaccttc agctgtttca
      481 cgaaaggcag ttggcatgct tgctcctact tggtacatgg catcgatcac ttcatccaca
      541 gggattttag attcgatacc tgccaaggcc atgtctgctg cgatgaaagc aaagctagct
      601 cccatggcat tacgtttgac acagggaact tcgaccaaac ctgcaacagg gtcacagatg
      661 aggcctagca tatttttaat gacaaaggca atagcttgac tggcctgata aggtgttcca
      721 cctgcagcca gagtcaaggc ggcagcactc atagcagagg ctgaaccaac ttcagcttga
      781 cacccaccct cagcacctga gatggaggca ttgtttgcga tgactagtcc aaaggcacca
      841 gcagcaaaga ggaaatccaa ttgttgctcg tggctgaggt ctaatttttc aatagcagca
      901 gtgagaacgg atggcagaca gccagcactt ccagcggttg gagtggcaca gaccaagccc
      961 attttggcat tgtgttcatt gactgcgatg gcatttcggg cagccgagag aatcgtataa
     1021 tctgacagag tttttccgtt ttcgatgtag tgatccaatt tggcagcatc tccacctgtc
     1081 aggccactac gagatttatt ttcattgagg ccaagttgga cagaggcttt cataacttcc
     1141 agattgcgtt ccatgagaag gaagacttct tcacgttcgc gaccggtcaa ttcaaactct
     1201 gttgtaatca tgagttctgc gacatttcct tgaaagtcca gatctgcttg ctcgaccaat
     1261 tctttgatag aataaaacat gcttcctcct atttaaagaa attgacattg tggagatgag
     1321 ggatttttcg gatttcttcg atagcctcat cacagttgcg actgtcaact tcgataatca
     1381 taatggcttt ttcaccagct ttttcacgag tgacattcat ctgggcgata ttgataccat
     1441 agcgggaaag cgcctctgta acaagggcaa tcatacctgg aatatcttga tgaacgatga
     1501 tgatagtcgg tgtattcata ttgagagaga cggcaaaacc attgagttcg gttacctgaa
     1561 tatttcctcc accgatagaa ataccagtca cgctgatggt cttgtgggca tttttaacag
     1621 taattttagt ggtgttaggg tgaggggcat tgctgtcttt ctgaatggtc cagacaatct
     1681 tgataccacg cttgtgggca atctccagac tgtttggaat ttcaggatca tctgtatcca
     1741 tgcctaaaat acctgcaaca agggctaggt ctgttccgtg accacgatag gtcttggcaa
     1801 atgagttaaa aagttggaat tcaacttctg tcggagtatc atcaaaaatg gaagagacaa
     1861 tcttcccaat acgaacagca ccagcagtat ggctactaga tgggccaatc ataactggtc
     1921 cgatgatatc aaagacagat tgaaaacgaa gtgatttcat cagtttcccc ttataaaaat
     1981 tcttatctct attatatcaa agaatgaggg gcttggcttt aattgtggat gaaaaccttt
     2041 ctaatacctc aaatagcata aaaatagtat cttttatgac aaaaaacacc ttatttaggg
     2101 aaataaaaaa taattttgta atatttctac ataaaagtgt caagaaacgg taatatttaa
     2161 agggtatgat agaactatag aaagaaggag aattttcgaa tatgaaatca ataactaaaa
     2221 agattaaagc aactcttgca ggagtagctg ccttgtttgc agtatttgct ccatcatttg
     2281 tatctgctca agaatcatca acttacactg ttaaagaagg tgatacactt tcagaaatcg
     2341 ctgaaactca caacacaaca gttgaaaaat tggcagaaaa caaccacatt gataacattc
     2401 atttgattta tgttggtcaa gagttggtta tcgatggccc tgtagcgcct gttgcaacac
     2461 cagcgccagc tacttatgcg gcaccagccg ctcaagatga aactgtttca gctccagtag
     2521 cagaaactcc agtagtaagt gaaacagttg tttcaactgt aagcggatct gaagcagaag
     2581 ccaaagaatg gatcgctcaa aaagaatcag gcggtagcta cacagctaca aacggacgtt
     2641 acatcggacg ttaccaatta acagattcat acctgaacgg tgactactca gctgaaaacc
     2701 aagaacgtgt agcagatgcc tatgttgcag gacgttacgg ttcatggaca gccgctaaaa
     2761 acttctggct taataacggc tggtattaag aaataacgag cagaataata tgaaaaggtc
     2821 gaggaaatcc ctcgactttt ttatatttcc ttcggttgag aatagatctc tacttctttt
     2881 ttgagatgag acagcataga agtttcctcg gtcagctcca gaagtgagaa acctttttgg
     2941 ataagtctag cgtcatccct acaaatcccg atacggacat agcagatagc aagtctatcg
     3001 tagtgcttct ttttcttggc atcctctagt aaagtatagc agatttgtcg acacatattt
     3061 ttgtcttgat ggtataagta aatggtggac agattgagta ggattgccat tcgtaagtca
     3121 tataaatgtt ggtagttttt atagacttct aagcgttgca agatttttcc agtaatgaga
     3181 tggaggtgat caatgggaaa gctgaaaagg atggtattga ggatttttag gtcattttca
     3241 taccatgtat cttgtttctc aattttttcc caaagttttg tgacagtctg tttcacttgg
     3301 tttgacagtt gctctgtacc atgttgacgg atatgaatga caatttccag catatccctt
     3361 atttcttcta taggcaggtc gtggtgggtc ttgagatagt cttggcatgt ttcaaaaaaa
     3421 tgaactagac tattactacc aatgatggaa gtcatattga gataagtttg catgatttct
     3481 gttcgttggc ttggttgata gagatggcag atgtagtcaa actcttcaaa actcatattg
     3541 atttgccgga gaagaaattc catgttttca tatttgggag ttgctttgcc actttcaatc
     3601 tttgataggc tagttcttga gatgacattt ccacagacct cttcttgggt caatcctttt
     3661 gactcgcgta tttctttata gacctttccg aaatcataac gcatgattca ctccttttcg
     3721 tgaaaaaagt taacaaataa taaaatccta ttaaaaatat tgtatcatag taatataaaa
     3781 agtaaaaaag aaagataaaa taagtttggt gtaattttaa tatggggtta atgagtacta
     3841 tgatgtagct agaaaagggg tggaaaaatg tttaaaaata aggaactttt tcgagtagtc
     3901 atgatatttg tgataggaat tatcctaatt ttaatgacac ccgtcttatt taaagttatg
     3961 atggaactat tttcaaaata aataggcctc tgttttaggc agaagcgtga atcaggaggt
     4021 taagtgatga actacaattt aaaatatctt ttatcaggaa tagttgtcct agtctttata
     4081 ggtttcctag tctggatgcg ttattttaat cagaggaagg aagaagagca ttcggatgtg
     4141 tcttttgaag cgaggttaga tagtgaggtc aagaccttat ataaacaact aggattgggt
     4201 gaggagcctc attatttctt agcgtatcgc tacctacatc cttggtttga tttggcgatt
     4261 ttaccaccaa cagttgaaat tttcttaact aaaatagcgc tggtgatggt aactccagat
     4321 gaactactga taagaaatct tgggaatggg ctgactttca caagtgagca tcatcgtgat
     4381 ttgcggcaag gacttatccg cattccaaaa tcagagatga aagaatttga gattcgtaac
     4441 tggaaaaaat tttttgtatt tggcgatttt ttgacaatta aaacaagcca acatagctat
     4501 tatttacagg ttagagatga tggacttcaa aaaggaagcc tttctaccaa acatttctca
     4561 gacttgaaga gccaagattt tctcggatta ttgacagata aacggacatt ctgatagacg
     4621 attgccctag ttgtttattg taaactttgc aaatttggct tgtaattttt tttataaaat
     4681 tcacttcgat tacttgacag ttattatatt ttcacttatt atatagataa aaggatggga
     4741 taagagttta aaaatttgaa agcgctatcc atattgttta ttgggctgat tcttgagtaa
     4801 tagttagagt gtgattaata ctactctggt tgttcaaaga cctgatacag aagagaacta
     4861 ttcttctagt taagagtaag ttcgttgttt aaaaatgaaa tgagatagaa ggaaggtgag
     4921 tccaatggac tacgatactt gttgataaat tactatagct gtttttacgg aacagctctc
     4981 gataatagga gaaataaatc atgagaaaat tagttaaaat tggtgttgca tctttaatgg
     5041 tatgtggtat acttgctact acaaatgctt tagcagtatg ggttgagggt ggtcaatgga
     5101 actatggagt aggttggaca ggaacttttg gatattctga ttacttacat tctactcgat
     5161 atcatacagc aactgttaga catgggggta gaacctctaa ggattatgca aaacctgagg
     5221 catgggctag agcttccctc accaagattc cgccaacagg aatggaatat ttctatggat
     5281 ttgagtaaaa ttcttccctc tcctatttgg agagggaaaa ctgtagtgga ggcactttta
     5341 tgaaaaaaat cagtaatttc tgtatgttac tcctgcttct gtgtaccact ttttttgttt
     5401 ttaatgtaaa ctatacacga gaagtggttc ggattcaaga aatgggaaag actgtagatt
     5461 ctttggattt gtatttgaaa gatattaacg aacctgcagc gtctgttctt cgattttttg
     5521 aggatgtatc aaaggagtat aaagtctcca tcatcaaaac agacagtggt gatgaggtgg
     5581 tcaagtctgg tgtttttgat aaagatacct tcccctacca agagtttggg atttcttctc
     5641 ttgattttac cacagatggt gaaggagtct atagtaataa agaaatttcc aataaacttg
     5701 gtacgattcc gacctttcta aaagccaaac ctattcagct tatgactttt caaacctata
     5761 tcaaggatac atctcgtagt ttaaatggtc gctatacgat aacttctaca caagagatgg
     5821 acaaggatag gattgtacag aaatggagcg attttttcaa gatagaccag gcgaccttgc
     5881 tagagccgac ctacaaaagt gcagtggaag tcataaatcg agatttgctt ttatctgcca
     5941 ttgtttttgt cttggctatt ttgcttcttg tgttagtgac agtgtatcaa ccgatgatgg
     6001 agatgaaaag agttggggta caaaaattac ttggttttca agatagggct gttttagctg
     6061 atgttgtaaa aggcaacctt tacctcctcc taggtggggc tcttgtgatc aatctaggcg
     6121 tgtttttctt gcttgattat aagccaaaag atttgtttcc tatgctgtgg ttgtctcatt
     6181 ttttgctgtt gcagctttat ctctttatca gttggttgac ttacctctta atccaaaaaa
     6241 tgacaatcag ctctctgctg aaaggttttt catctttcaa atttggtctt atcttcaatt
     6301 atgtgatgaa aatagggaca actattttac tgacggcctt actgattggg gtgggcagaa
     6361 gtttagaaca agaaaacaaa gaacttgctt atcagcaaca gtgggtaagt caaggtaatt
     6421 acctgacctt agaaaccttc aaactcaatg ataatctgtg gcaagaagag ctagcagggt
     6481 cagggaaatc tacagattat ttctatcgat tttatcagga tttggtagaa aaaacgcagg
     6541 cgggctatgt gcagagtagc agtcttcctg taaaaaattt tgtccaatca gaacagattc
     6601 agcaatatca gttaacagat acggtggatg tttactatgc caatcgcaat tttctaaaga
     6661 gcaagggatt caagctacca gataccggta ttaaaaaagt tattttgatg ccagcaagta
     6721 cgaaaggtga agaagataaa aatcagctct tggggaagtt aattgccttt cattcgatga
     6781 agtatgaaga gcagcaaaaa cgaacgatag aggagatgga tgtcgagatt gcctattatg
     6841 aaggagattg gtcatttttc ccatatagtg ataagcgaaa ggaaaatctc tccaatccaa
     6901 ttattagctt ggtcaatgat tctgatatga tgtgggatga gaaagcctcc ctgtcaacaa
     6961 ctggcttaaa taatccgatt aaaattgaaa atacggttca acatcaaaaa gagattacag
     7021 agttagttga gaaattgtca gatggaaatt atttaaaatt ttcatctatt caagccattc
     7081 aacaagagaa agtggattct tatagagatg ctgttcggaa ttttaaccta ctctttgctt
     7141 tgtttggtct ccttagcatg atgatttcct acttcttact agtaacaact ttcttattga
     7201 agcgcaggga tatcattacc aagaagttta tggggtggaa actggtcgat cgctaccgtc
     7261 ctctcctcgt tctgctcttg ctgggctata gtttccctct tctagtcttg attttctttg
     7321 cccatgcgtt cttaccactt ctactgtttg caggttttac atgtctggat atactatttg
     7381 tgctaggctt agcttctagg atggagaaaa gaagtctagt agagttattg aaagggggca
     7441 tcttatgatt gagttgaaaa atattaccaa aaccattggg ggaaaagtga ttttggataa
     7501 cttatctctc aggattgatc agggggcttt ggtagccatt gttggcaaaa gtggcagtgg
     7561 caagtcgacc ttgttaaatt tattgggttt gatagatggt gattatagcg gacggtatga
     7621 gatttttggt cagacaaatc tagcggttaa ttctgctaag tcgcaaacaa taatccgtga
     7681 acatatctct tatctgtttc aaaattttgc cctgattgat gatgaaacgg tcgagtacaa
     7741 tctcatgctg gcgctgaaat atgtgaaatt gcctaagaaa gacaagctca aaaaggtgga
     7801 agagatttta gagagagtag gtttgtcagc tactttgcat caaagggtct ccgagttgtc
     7861 tgggggcgaa caacaacgaa ttgcagttgc tagagccatc ttaaaaccca gccagctgat
     7921 tttagccgat gaacctacag gttcgctgga tcctgaaaat agagatttgg tcttgaagtt
     7981 tctcttagag atgaatcgag aagggaaaac agtcattatt gtgacccacg atgcttatgt
     8041 agcccaacaa tgtcatcgtg tcattgaatt gggtgaggga aaatgagttc attcagctcc
     8101 ttttgactgg ctgaatactc atgttttcca gagaaaaata gcataaatac gcctaggaat
     8161 gacattttat atagtatttc taggtttttt ttgtttcaaa ttgaaaattt tttcaattta
     8221 ggcttgacaa aggatgagta taggagtatt atttatacaa taaaaaagaa taaacataaa
     8281 ggaggctttg ttatgaataa gatgaagaag gtgttgatga cgatgtttgg tttagtgatg
     8341 ctccccctac tatttgcttg tagtaacaat caatcggctg gaattgaagc catcaagtcc
     8401 aaaggaaaat tggttgtagc cctcaatcca gattttgctc catttgaata tcaaaaagtg
     8461 gttgatggga aaaatcagat tgtgggttca gatatcgaat tagccaaggc tatcgcaaca
     8521 gaactaggtg tcgaattgga actatctcct atgagttttg acaatgtact ggctagcgtt
     8581 caatcaggaa aagctgacct tgccatatca ggtgtttcta agacagatga acggagcaag
     8641 gtgtttgatt tttcaactcc ctactatact gcaaaaaata aactcattgt caaaaaatct
     8701 gatttagcca cttatcagtc tgtcaacgat ttggcgcaga aaaaggtcgg agcgcagaaa
     8761 ggttcgattc aagagacgat ggcgaaagat ttgctacaaa attcttccct cgtatctctg
     8821 cctaaaaatg ggaatttaat cacagattta aaatcagggc aagtggatgc cgttattttt
     8881 gaagaacccg ttgccaaggg atttgtggaa aataatcctg atttagcaat cgcagacctc
     8941 aattttgaaa aagagcaaga tgattcctat gcggtagcca tgaaaaaaga tagcaaggaa
     9001 ttgaaagagg cagtcgataa aaccattcaa aagttgaagg agtctgggga attagacaaa
     9061 ctcattgagg atgcctttaa agcgtccatt gaaaaataga aagaaggaaa agaacatgag
     9121 taaagaaaaa gtaattttgg cctattctgg cggtttagac acatcagttg ctattacctg
     9181 gctgaagaaa gactatgatg ttgttgcagt ttgtatggat gtgggtgaag ggaaagactt
     9241 agatttcatc catgataagg ctcttaaggt tggggcagtc gaatcttatg tcattgatgt
     9301 taaggacgaa tttgctacag attatgtgct agtagctctc cagtcacatg cctactatga
     9361 acaaaagtac cccttggtat ctgccttgag ccgccctctt atttctaaaa aattggttga
     9421 aatagcgcat cagacaggag cgactacaat tgctcatggt tgtacaggta aggggaatga
     9481 ccaagtacgt tttgaagtat cgattgcagc cttggatctc aatttaaaag tgattgctcc
     9541 agttcgtgaa tggaaatggt ctcgggagga agaaatttat tatgccaagg aaaatggggt
     9601 gcctgttcct gctgaccttg acaatcccta ctctgtcgac caaaatcttt ggggacgtgc
     9661 caatgagtgt ggtattttgg aaaatccatg gaatcaggct ccagaagaag cctttggcat
     9721 cacaacttct ccagagcaag ctccagatat gccagaatac attgagattg agtttagtga
     9781 aggtgttcca gtttccctca atggagaagt gttaaaattg gcagatttga ttcaaaaact
     9841 caatgaaata gctggaaaac atggggttgg acggattgac cacgtggaaa atcgcttggt
     9901 tgggattaaa tcaagagaaa tctatgagtg tccaggtgca gtgaccctgc tgacagctca
     9961 taaggaaatt gaggacttga cgcttgtgag agaagtggct cattttaaac caatcataga
    10021 aaatgaatta tcaaatctca tttataatgc tttgtggttt agttcggcta cacaagcctt
    10081 gattgcttat attaaagaga ctcaaaaagt tgtcaatggg actgcaaaag tcaaactgta
    10141 taaggggagc gctcaggtcg tggctcggaa atcaccaaat tctctttacg atgaaaattt
    10201 ggcgacgtat actagtgcag ataccgttga ccaagatgcg gctgttggct ttattaagtt
    10261 atggggactt ccaaccaagg ttcattct
//
