LOCUS       NZ_LN831790            10555 bp    DNA     linear   CON 08-APR-2024
DEFINITION  Streptomyces leeuwenhoekii strain type strain (C34 = DSM 42122 =
            NRRL B-24963) chromosome 1.
ACCESSION   NZ_LN831790
VERSION     NZ_LN831790.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces leeuwenhoekii
  ORGANISM  Streptomyces leeuwenhoekii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Gomez-Escribano,P.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2015) JIC, Department of Molecular Microbiology,
            Norwich Research Park, Norwich, NR4 7UH, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001013905.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 03:45:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,096
            CDSs (total)                      :: 7,005
            Genes (coding)                    :: 6,806
            CDSs (with protein)               :: 6,806
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 82 of 199
            Pseudo Genes (incomplete)         :: 160 of 199
            Pseudo Genes (internal stop)      :: 10 of 199
            Pseudo Genes (multiple problems)  :: 49 of 199
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:33:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5330378
            Orig. end                         :: 5340933
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LN831790.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10555
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="25"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10555
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10555
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5555](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5555
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(52..1512)
                     /locus_tag="BN2145_RS24280"
                     /old_locus_tag="ABM27_RS23825"
     CDS             complement(52..1512)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /GO_process="GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bac_transf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1146: sugar transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028528.1"
                     /locus_tag="BN2145_RS24280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS23825"
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="WP_047122016.1"
                     /sec_met_domain="Bac_transf (E-value: 1.3e-58, bitscore:
                     196.1, seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAESTVPSPAGQPRDLALSPLRILPPRTRGGGFALPARRPAARP
                     ASRLPLLAADGTAALLGSLTLTGAPDRPLLAAPLVAATLLLRPRGLPPVPAGVLDELPS
                     VCGRIAVAWLALAALLAAYTPHQALSADSLLLGFTVQAAAGCAGRGAVHWRRRTTLRNR
                     PRAALVIGPAGVAQRVAAAVLRHPRCGVRPVGVVTGGPFGSSLLGGPGTGAAGLPVLGT
                     EEEVRRAVARNGVREVLVVHPSVRTRQAPLLRALAASGCAVWEVDPRSPSSGSRDEIAG
                     FSRRRLEAGAGRPGGALGKRVLDVVVSGVLLLAAGPLLLACAVVLRVSEGPGVVFRQVR
                     VGQDGKPFTLLKFRTHRPVDEHESATRWSVADERRMPWFCRFLRRTSLDELLQLWNVFR
                     GDMSLVGPRPERPYFVTKFSETYPGYAARHRMPAGITGLAQIHGLRGDTSIEDRARFDN
                     AYIDNWSLWQDVCILLRTAVTLVRPTGS"
     gene            complement(1509..2687)
                     /locus_tag="BN2145_RS24285"
                     /old_locus_tag="ABM27_RS23830"
     CDS             complement(1509..2687)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116489.1"
                     /locus_tag="BN2145_RS24285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS23830"
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_029385995.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 7.8e-34,
                     bitscore: 115.4, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLPATGPAPRVLHLTQPVDGGVARIVTDLARAQLAAGWDITVAC
                     PDGPLAASLRSLGAEVRPWPATRSPGPSLPAETWRLARIVEDVRPDLVHAHSAKAGLAG
                     RLAVRARIPTVFQPHAWSFEAVGGVPAVLALGWERWAARWAARVVCVSEAERRRGVSAG
                     IRGRWAVVPNGVDPDHFRPGPAVRPPLLADLGPRARLVVCVGRLCRQKGQDVLIAAWDR
                     IAERVPDARLVLVGDGPERARLRALAPASVRFTGAVADAAPWYRAADVVVLPSRWEGIA
                     LAPLEAMACGRPVVLTDVDGAREVLPPALAPHCLVPPTDPAALAGAVSALLLDPALRAS
                     TARQGRAHVLSAHDVRRTARAVAAVYGELLGGGPDAAGPAVVPTEYRESIQS"
     gene            2989..3243
                     /locus_tag="BN2145_RS24290"
                     /old_locus_tag="ABM27_RS23835"
     CDS             2989..3243
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529516.1"
                     /locus_tag="BN2145_RS24290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS23835"
                     /product="chaplin"
                     /protein_id="WP_029385994.1"
                     /transl_table=11
                     /translation="MSRIAKGLVLTSVAAGAVTGTTGVAVAADAGAGAGAKATAADSPG
                     GLAGSAVQVPVYVPVNVCGNTVDVGGLLNPAFGNKCKNA"
     gene            3523..4380
                     /locus_tag="BN2145_RS24295"
                     /old_locus_tag="ABM27_RS23840"
     CDS             3523..4380
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976096.1"
                     /locus_tag="BN2145_RS24295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS23840"
                     /product="hypothetical protein"
                     /protein_id="WP_047122017.1"
                     /transl_table=11
                     /translation="MRLPARRIAASAFCATLLLAVTGPAATAADGDSVRERTSAASRAP
                     LPDVGALTAQVQSLSGLGGVVAPVSDLLGAVLKADDGRLTKEQAEQFSKAVEDALAKAQ
                     ATGMPIAPEAAAPGTTTPDTATPGTTAPGVGTPAQPPVALQPPAVAQPNDDAPATTLPA
                     PLAPAPLAPAPLAPAPAAPAAVAPAADESGTASTGLIGAALDALRKSIETLIGASTSAQ
                     PEQVSPAAAGVVTDVVNVVAATLFGGGLPAPDLAGLPPLPAAPNGTTPANTPADTPVNA
                     PATP"
     gene            4670..4951
                     /locus_tag="BN2145_RS36280"
     CDS             4670..4951
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056481.1"
                     /locus_tag="BN2145_RS36280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_029385992.1"
                     /transl_table=11
                     /translation="MKPLKAAAAVAGSLALAGAAGPAYAHNDVAELTHAVDHVAGVVTE
                     EPVIGLPVESKPGESRTEDKSSALRTVKTLIDEVGSVGALLGGLPARG"
     gene            5001..5555
                     /locus_tag="BN2145_RS24305"
                     /old_locus_tag="ABM27_RS23850"
     CDS             5001..5555
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028525.1"
                     /locus_tag="BN2145_RS24305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS23850"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_029385991.1"
                     /sec_met_domain="melC (E-value: 6.7e-40, bitscore: 135.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVSEREAARDAGREPADPAGGGPADATRRRLARRLLVPALAAAF
                     VPVAAAPGSPRPAKSAGDSKAARTDRRGTAGRARTATRPLAAPDDGAFDETYLGRRIRG
                     VRTPDAAGGPGSWHVTVDGRPLHLMRRADGTWMSMVDHSRSYPTPLEAARAAVDALPPG
                     QRLSGTGGGHGHGGGRHGVHA"
     gene            5539..6405
                     /locus_tag="BN2145_RS24310"
                     /old_locus_tag="ABM27_RS23855"
     CDS             5539..6405
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116480.1"
                     /locus_tag="BN2145_RS24310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS23855"
                     /product="tyrosinase family protein"
                     /protein_id="WP_029385990.1"
                     /transl_table=11
                     /translation="MVYTRKDVSTLTRAERQRFVRALLEVKRRGEYDEFVRMHIEYFVP
                     DGEDGLRVAHMTPSFLPWHRQFLLELERALQRVDASVTVPYWDWTKDRRATSVPWTADL
                     LGGNGRSSDHQVTTGPFAYREGNWTLRYNITDTRFLTRDLGRRRAPIQLPTKSDLEWAL
                     KDPVYDAAPWDSTSSKGFRNKLEGWGPGRGSTSWRNHNRVHRWVGGVMVGGASVNDPVF
                     WLLHAFVDLQWSRWQRRHRTTRYLPAQPPGESNPQHGRIIARHQKLPPWDVTPDQLEDL
                     SHIYRYG"
     gene            complement(6512..6703)
                     /locus_tag="BN2145_RS24315"
                     /old_locus_tag="ABM27_RS23860"
     CDS             complement(6512..6703)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018896056.1"
                     /locus_tag="BN2145_RS24315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS23860"
                     /product="hypothetical protein"
                     /protein_id="WP_047122617.1"
                     /transl_table=11
                     /translation="MSPAPVLGLLPTEPGPVAGCATCQGLAREREAARAARDGSRVSDC
                     NVLIRAHPHGPRASGRGE"
     gene            <6716..7012
                     /locus_tag="BN2145_RS24320"
                     /old_locus_tag="ABM27_RS23865"
                     /pseudo=""
     CDS             <6716..7012
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263401.1"
                     /locus_tag="BN2145_RS24320"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS23865"
                     /product="XRE family transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="NLPNASLNPSSPLTLDLQLEGNTVTGTWTEQTAPDGYYRGARYFG
                     ALQMLVEPTGRRMAGKWVGFGKEFDVNTGPWELRLMDTSTSKATLEAYSRPPE"
     gene            complement(7094..7987)
                     /locus_tag="BN2145_RS24325"
                     /old_locus_tag="ABM27_RS23870"
     CDS             complement(7094..7987)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612292.1"
                     /locus_tag="BN2145_RS24325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS23870"
                     /product="IS982 family transposase"
                     /protein_id="WP_029385988.1"
                     /transl_table=11
                     /translation="MTTELDTLLTALYVHIDDHLKTPRRRGRPPKLTDAELITLAVAQA
                     MPGFHCEARWLRFAHAHLHGMFPCLPQRPAYNKRLRAALGLVKQAIRSLAADTDLWLDD
                     VWIVDSTPVECACSRETVKRSDLAGWAGYGYCRSHSRFYWGLKLHLVCTPAGLPVTWAL
                     ADPKIDERQILAALIDNEPHLAAHRPGLLILANKGYTATELDRFLAARGISLLRPSYRN
                     RTPRPGEALLKTVRQLIESVNDTLKGQLDLEQHGGRTIEGVGVRVAQRILAMTCAIWHN
                     RTIGAPVTRSLIACDH"
     misc_feature    complement(7973..7975)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(8155..8733)
                     /locus_tag="BN2145_RS24330"
                     /old_locus_tag="ABM27_RS23875"
     CDS             complement(8155..8733)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060600.1"
                     /locus_tag="BN2145_RS24330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS23875"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_029385987.1"
                     /transl_table=11
                     /translation="MVRRNDQRRAALVDAAIEVLAREGARGLTFRAVDAEAAVPAGTAS
                     NYFASRDDLFTEAGARVYERLQPDEATIARQRAADRHRGTYAELMRELVGRIASFRTGY
                     LALLELRLEATRRPELRKVLTERVRADVDANVAYHEASGLPGDATAVKLLMLTLNWLIV
                     EQLTLPDVFTEEEREQLVTAAVERIVAAE"
     misc_feature    8795..8809
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.18"
                     /tool="antismash"
     gene            8803..9396
                     /locus_tag="BN2145_RS24335"
                     /old_locus_tag="ABM27_RS23880"
     CDS             8803..9396
                     /GO_function="GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006348705.1"
                     /locus_tag="BN2145_RS24335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS23880"
                     /product="dihydrofolate reductase family protein"
                     /protein_id="WP_029385986.1"
                     /transl_table=11
                     /translation="MRKLVYYIGVSLDGRIAGPGGEFDFFPQGDEQQAAAYSTWMNALY
                     PETIPTAYRAAVGVADAPNRRFDTVVMGLGTYRLSVDNGITSPYAHLRQYVVSSTLKPD
                     TDPAVTVVPSDPRALIRELKGEMAAGPDIWLCGGGRLAGTLLPEIDELLIKTYPVVAGT
                     GILVVDGAFDPTVFNVAERTAFPNGVTLTRLTRR"
     gene            complement(9408..10267)
                     /locus_tag="BN2145_RS24340"
                     /pseudo=""
     CDS             complement(9408..10267)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377534.1"
                     /locus_tag="BN2145_RS24340"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="IS5 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MVGPGVPLTDAQWARIEPLLPDRTPKRGGRWRDHREVINAIAFKF
                     QTGTQWVHLPEKYGNWRGVYNRLRMWAVDGTWERVFTALIAQADAEEDLNWAVSVDSTI
                     VRAHQHAAGARKKGPRSASPTTTPSAGPAAD"
ORIGIN
        1 ggggacaccg gtgcgcgggc cggtgcgggg gcagcgcgtg gctcaccgcg gtcagctccc
       61 ggtggggcgc acgagcgtga cggccgtgcg cagcaggatg cacacgtcct gccacagcga
      121 ccagttgtcg atgtaggcgt tgtcgaagcg ggcccggtcc tcgatggagg tgtcaccgcg
      181 cagcccgtgg atctgggcga gcccggtgat gccggccggc atccggtgcc gggccgcgta
      241 accgggatag gtctcgctga acttggtgac gaagtagggg cgttcgggcc gcggcccgac
      301 caggctcatg tcgccgcgga acacgttcca caactggagc agctcgtcca gcgaggtgcg
      361 gcgcaggaag cggcagaacc acggcatccg ccgctcgtcc gccacactcc accgggtcgc
      421 cgactcgtgc tcgtccaccg gccggtgggt gcggaacttc agcagcgtga acggtttccc
      481 gtcctggccg acgcgcacct gccggaacac cacccccggc ccctcgctga cccgcagcac
      541 caccgcgcac gccagcagca gcggaccggc cgccagcagc agcaccccgg acaccaccac
      601 gtccagcacc cgcttgccca gggcgccgcc cggccgcccc gcccccgcct ccagccgccg
      661 gcgggagaac ccggcgatct cgtcgcggct ccccgacgac ggggaccgcg ggtcgacctc
      721 ccagaccgcg cagccggacg cggcgagcgc ccgcagcagc ggcgcctgcc gcgtccgtac
      781 cgagggatgc accaccagca cctcccgcac cccgttccgg gcgaccgccc gccggacctc
      841 ctcctccgtg cccagcacgg gcagccccgc cgcgcccgtc ccgggcccgc cgagcaggga
      901 ggacccgaac ggcccgccgg tcaccacacc caccgggcgc accccgcagc gcgggtggcg
      961 cagcaccgcc gcggccacgc gctgcgccac cccggccggc ccgatgacga gcgcggcgcg
     1021 cggccggttg cgcagcgtcg tacggcgccg ccagtgcacc gcgccgcggc ccgcgcagcc
     1081 cgccgccgcc tgcacggtga aaccgagcag caggctgtcg gcggacagcg cctggtgcgg
     1141 ggtgtacgcc gccagcagcg cggccagcgc gagccaggcg acggcgatcc ggccgcacac
     1201 ggagggcagt tcgtccagga caccggccgg tacgggggga agcccacgcg ggcgcagcag
     1261 cagcgtcgcc gccaccagcg gggccgcgag gagcgggcgg tccggggcac cggtgagcgt
     1321 cagggagccc agcagggccg cggtgccgtc cgcggcgagc agcggcagcc gggaggccgg
     1381 gcgcgccgcg gggcgccggg cgggaagcgc gaacccgccc ccccgcgtgc gcggcggcag
     1441 gatccggagg ggggagaggg ccaggtcccg cggttgccct gcgggggagg ggacggtgct
     1501 ttccgcggtc acgactggat ggactccctg tactcggtgg ggacgacggc cggacccgct
     1561 gcgtccggcc cgccgccgag gagttcgccg tacacggcgg ccacggcccg ggccgtgcgc
     1621 cgaacgtcgt gggcggacag gacatgggcc cgcccctggc gagccgtcga ggcgcgcagt
     1681 gccgggtcga gcagcagcgc gctgacggcg cccgccagcg ccgcggggtc cgtgggcggc
     1741 acgaggcagt gcggcgcgag cgcgggcggc aggacctccc gggcgccgtc cacgtcggtc
     1801 agcaccacgg gccgcccgca ggccatggcc tccagcgggg ccagcgctat gccctcccag
     1861 cgcgagggca gtacgaccac gtcggcggcc cggtaccagg gggcggcgtc ggcgacggcc
     1921 cccgtgaacc gcacggacgc cggggccagc gcccgcagcc gcgcccgctc cggcccgtcc
     1981 ccgaccagca ccagccgggc gtccgggacc cgctcggcga tccggtccca ggcggcgatc
     2041 aggacgtcct gccccttctg ccggcacagc cgccccacgc agaccacgag ccgggcccgc
     2101 ggaccgaggt ccgcgagcag cggcggccgt acggcggggc cgggccggaa atggtccggg
     2161 tcgacaccgt tcggcaccac cgcccaccgc ccgcggatcc ccgccgacac cccccggcgg
     2221 cgctccgcct cgctgacgca caccacccgc gcggcccatc gcgccgccca ccgctcccag
     2281 ccgagcgcca ggacggcggg gaccccgccg accgcctcga acgaccacgc gtgcggctgg
     2341 aacacggtcg ggatccgcgc ccggacggcg agccgccccg ccagacccgc cttcgcgctg
     2401 tgcgcgtgca ccagatcggg ccgtacgtcc tcgacgatcc gcgcgagccg ccatgtctcg
     2461 gcggggagcg acggccccgg cgagcgcgtc gcgggccacg gccgcacctc cgcgcccagc
     2521 gaacgcagcg acgcggcgag gggaccgtcg gggcaggcga ccgtgatgtc ccacccggcg
     2581 gcgagctgtg cccgtgccag gtccgtcacg atccgggcga ccccgccgtc cacgggctgg
     2641 gtgaggtgca ggacccgcgg cgcaggtccg gtcgctggca ggtgcatgcg cgcttcctcg
     2701 ctcacggatg gtgctcggga cgggcgggaa cgcgccgcgg agggccgccc gaatgtgatg
     2761 cgccggcacc ggggcgccgg catcggaggt gcgtcggacg gtgtgtcacc ctagccgcaa
     2821 gacgtagcta atactgcgaa accggataag tgtgtcggat cgggtgacaa agcgggccgg
     2881 gttgcatgtt cgtcacccgg ttggtggcgc aaccggtggg gggaccatgc gttgacactg
     2941 cgccgggcgt tcgcccggat ggtcgtcaac cccaaggagc acctctccat gtcgcgtatc
     3001 gcgaaaggcc tggtcctgac ctctgtcgcc gccggagcgg tgacgggcac cacgggcgtc
     3061 gccgtcgccg ccgacgccgg cgcgggcgcc ggtgcgaagg cgaccgccgc ggactccccg
     3121 gggggactgg ccggcagtgc cgtgcaggtc cccgtgtacg tcccggtgaa cgtctgcggc
     3181 aacacggtcg acgtcggcgg cctgctgaac ccggccttcg gcaacaagtg caagaacgcc
     3241 tgaggccctt tccccttctc ctgccgtccg tccggcccgc ggctcgtccg tggcggcggc
     3301 gaccggcggc cgtgtgcgcg agaccgcccg gcgttcgccc gttgccccga atgggggtgg
     3361 tgcgaacggc ccggcgcgct ggccccgtca cccggtctga gcaggtgcgg acggctcgcg
     3421 gcgacggccg gacggcaacg ctgggcaagg gatcggttgc agagcgcgag ggcggcttcc
     3481 acggtggcag tacgaccctg cacaccaccg aaaggaaccc tcatgcgtct gcccgcacgg
     3541 cgtatcgccg cctccgcgtt ctgcgccacc ctcctgctcg cggtcaccgg gccggcggcg
     3601 acggcggccg acggcgactc ggtccgggag cggaccagcg cggcgtcccg ggcgccgctg
     3661 cccgacgtcg gcgcgctcac cgcccaggtc cagtcgctca gcggtctggg cggcgtggtc
     3721 gcgccggtca gcgatctgct cggcgcggtg ctcaaggccg acgacggccg cctcaccaag
     3781 gagcaggcgg agcagttctc caaggccgtg gaggacgccc tcgccaaggc acaggcgacg
     3841 gggatgccga tcgcaccgga ggcggcggcg ccgggcacca ccacgccgga caccgccacg
     3901 cccggcacca cggcccccgg cgtcggcacg ccggcccagc cgccggtggc gctccagccg
     3961 cccgccgtcg cccagccgaa cgacgacgcg ccggccacca cgctgccggc cccgctcgcc
     4021 cccgccccgc tcgcccctgc tccgctcgcc ccggccccgg ctgcgcccgc cgcggtcgcc
     4081 cccgccgcgg acgagtccgg cacggcgtcg accggtctca tcggtgcggc gctggacgcg
     4141 ctgcggaagt cgatcgaaac cctgatcggg gccagtacgt cggcccagcc cgagcaggtg
     4201 agcccggcgg ccgccggcgt cgtgaccgac gtcgtcaacg tggtcgcggc caccctgttc
     4261 ggcggcgggc tgcccgcccc cgacctggcc ggcctgcccc cgctgcccgc cgccccgaac
     4321 ggcacgacgc cggcgaacac cccggcggac accccggtga acgcaccggc gaccccctga
     4381 cggtcccgcc ccaccgggcc gccgcacccg ccttccgggc gggtgcggcg gccttttcgc
     4441 gtctcgcccc cgttccggcg cgcacaggac ccggtacgca ttctcatccg atttccgtta
     4501 cgggtcctga tatgaggatg agaaacacgg gtgagacaca ccgttcgttc gggtgggtca
     4561 cggaaaggtc gcggcactcg gggtttccgg tccgggcggc actcgttagg cacggcacca
     4621 cccctttccg cttttgcgcg gtgagggatt cgccgaagaa aggaagacga tgaagcccct
     4681 gaaggccgcc gccgcagttg ccggatccct ggccctggcc ggagccgccg ggcccgcgta
     4741 cgcccacaac gacgtcgccg aactcacgca cgccgtcgac cacgtcgcgg gcgtggtcac
     4801 cgaggagccg gtgatcggcc tgccggtgga gtccaagccc ggggagtccc gcaccgagga
     4861 caagtcgtcc gccctgcgca ccgtgaagac cctgatcgac gaggtgggct ccgtgggcgc
     4921 cctgctcggc ggcctgccgg cgcggggctg aaccccgttc gccatcaccg acggacggac
     4981 accggacgag gagcacgatc atggccgtca gcgagcgcga ggcagcccgg gacgcggggc
     5041 gggaaccggc ggacccggcc ggaggcggcc ccgccgacgc cacccgccgc cggctggcgc
     5101 gccggctgct cgtccccgcc ctggccgcgg cgttcgtccc cgtggccgcc gcccccgggt
     5161 ccccccggcc cgcgaaatcc gcgggggatt cgaaagccgc acggacggac cggcgcggca
     5221 cggccggccg cgcgcggaca gcgacccgcc ccctggccgc cccggacgac ggcgcgttcg
     5281 acgagaccta cctcggccgg cgcatccgcg gcgtcaggac gccggacgcg gccggcggcc
     5341 ccggcagctg gcacgtcacg gtggacgggc gcccgctgca cctgatgcgc cgcgccgacg
     5401 gcacctggat gagcatggtc gaccactccc gctcgtaccc caccccgctg gaggcggccc
     5461 gcgcggccgt cgacgcactc ccgccggggc agcggctgag cgggacgggc ggcggacacg
     5521 ggcacggggg aggccgccat ggtgtacacg cgtaaggacg tcagcaccct gacccgcgcc
     5581 gagcggcagc ggttcgtacg ggccctgctg gaggtcaaac gccggggcga gtacgacgag
     5641 ttcgtgcgca tgcacatcga gtacttcgtc cccgacgggg aggacggact gcgcgtggcc
     5701 cacatgaccc cgtccttcct gccctggcac cggcagttcc tgctggaact ggagcgggcg
     5761 ctgcagcggg tggacgcctc ggtcaccgtg ccctactggg actggaccaa ggaccgccgc
     5821 gccacctccg tgccctggac cgccgacctg ctcggcggca acggccggtc ctcggaccac
     5881 caggtgacga ccgggccgtt cgcctacagg gagggcaact ggaccctccg gtacaacatc
     5941 accgacaccc ggttcctcac ccgggacctc ggccgccgcc gcgcccccat ccaactgccc
     6001 accaagagcg acctggaatg ggcgctgaag gaccccgtct acgacgccgc gccctgggac
     6061 tcgacgtcct ccaaggggtt ccgcaacaag ctggagggct ggggacccgg gcggggcagc
     6121 acctcctggc gcaaccacaa ccgggtgcac cgctgggtcg gcggggtcat ggtcggcggc
     6181 gcctccgtga acgaccccgt cttctggctg ctgcacgcct tcgtcgacct ccagtggtcc
     6241 cgctggcaac gccgccaccg caccacccgc taccttccgg cccaaccacc cggcgagagc
     6301 aacccccaac acggacgcat catcgcccgc caccagaaac tccccccgtg ggacgtgacc
     6361 ccggatcagt tggaggacct cagccacatt taccggtacg ggtgaggcag ggaccggagc
     6421 taaagcccag cccccgtaag ccggaatggc ctgccgggcc ggggatttct ccttcggtcg
     6481 ggagctatcg aagcgtcggg tgctccgagg gtcattcacc gcggccgctg gcccgggggc
     6541 cgtggggatg ggcgcggatg aggacgttgc agtccgagac gcgcgagccg tcccgtgcgg
     6601 cccgtgcggc ctcccgctcc cgggcgaggc cctggcaggt cgcgcacccc gccaccgggc
     6661 cgggctcggt tgggagcagc cccaggacgg gggccgggct catggtggta cggggaatct
     6721 gccgaacgcc tccctcaacc cgagcagccc tctgacgctc gatcttcagt tggagggcaa
     6781 caccgtcacc ggcacatgga ccgaacagac cgcaccggac gggtactacc gtggcgcccg
     6841 gtacttcggc gcgctccaga tgctcgtcga gccgaccggc cggcgcatgg cgggcaagtg
     6901 ggtcggcttc ggcaaggaat tcgacgtgaa caccgggccg tgggaattgc ggctcatgga
     6961 cacatcgacc agcaaggcga cgttggaggc gtacagtcgc ccacccgagt gacgcccgga
     7021 ggtgacggtt cgctccctgc acggaacccg ctcgccgtgg cacccggggc tagacgagta
     7081 gttccgatgt ggctcagtgg tcgcaggcga tcagtgagcg ggtgacgggt gcgccgatgg
     7141 tgcggttgtg ccagatcgcg caggtcatcg ccaggatccg ctgagccacg cggacaccga
     7201 cgccttcgat cgtgcgtccg ccgtgttgtt ccaggtcgag ctggcccttg agggtgtcgt
     7261 tgaccgactc gatcaactgc cgcaccgtct tgagcagggc ttctcccggc cgcggggtgc
     7321 ggttgcggta ggagggccgc agcagactga tgccgcgggc ggccaggaag cggtcgagtt
     7381 cggtggcggt atagcccttg ttcgccagga tcagcaggcc gggccggtgg gcggccaggt
     7441 gcggttcgtt gtcgatgagg gcggccagga tctgacgttc gtcgatcttg gggtcggcca
     7501 gggcccaggt gaccggcagt ccggccgggg tgcataccag gtgcagcttc aggccccagt
     7561 agaagcgcga gtgggatcgg cagtagccgt agccggccca gccggccagg tcggagcgtt
     7621 tgacggtctc gcgggagcag gcgcattcga cgggggtgga gtccacgatc catacgtcgt
     7681 ccagccatag atcggtgtcg gcggccagcg atcggatggc ctgtttgacc aggcccagag
     7741 cggctcgcag ccgtttgttg taggccgggc gctgaggcag gcaggggaac atcccgtgca
     7801 ggtgggcgtg ggcgaagcgc agccagcggg cctcgcagtg gaaacccggc atggcctggg
     7861 ccacagccag agtgatcagt tccgcgtcgg tcagcttcgg cggccggcca cgccgtcgcg
     7921 gggtcttcaa atggtcgtcg atatgcacgt acagtgccgt caagagggtg tctaactcgg
     7981 tcgtcacaac ccgatgttgg acaccctccc tacgtctgag aaccccgcat tcggaactac
     8041 tcgtctaggc cgtttctttc ggatcagcgt agggaagcaa gggcgggcac atgccgaggc
     8101 cggtccggtc tgcttccgct gcgccgggcg gcgtcatttg tggggccaag ccctctactc
     8161 cgcggccacg atgcgctcca ctgcggccgt caccagttgt tcacgctctt cctctgtgaa
     8221 gacgtccggc agggtgagct gctcaacgat cagccagttc agcgtcagca tgagcagctt
     8281 gaccgccgtg gcgtcgccag ggaggccgga agcctcgtga taggcgacat tggcgtcgac
     8341 atcggcccgg actcgctcag tgaggacctt gcgcagttcg gggcggcggg tggcctcaag
     8401 gcggagttcc agcagcgcga ggtaaccggt gcggaaggag gcgatgcggc cgacgagctc
     8461 gcgcatcagt tcggcgtagg tcccccggtg gcggtcggcc gcccgctggc gggcgatcgt
     8521 ggcctcgtcg ggctggagtc gctcgtaaac ccgggcgccg gcctcagtga acaggtcgtc
     8581 gcggctggcg aagtagttgg acgccgtgcc ggcgggaacg gctgcctcgg catccacagc
     8641 ccggaaagtc aggccgcgtg cgccttctcg ggccagcacc tcgatcgccg cgtcaacgag
     8701 ggccgcgcgc cgctggtcgt tccgtctcac cattgacacc actccaggtg tagtactacg
     8761 ttcaaaccac ttcagtcata gtactacgtg tgggggatca ctatgcgaaa gctcgtgtac
     8821 tacatcggcg tctcgctcga cggccgcatc gccggccccg gcggcgaatt cgacttcttc
     8881 ccgcagggcg acgagcagca ggccgctgcc tactcgacct ggatgaacgc gctgtacccg
     8941 gagaccatcc cgaccgctta ccgcgcggct gtcggcgtcg ccgacgcacc taaccggcgt
     9001 ttcgacaccg tcgtcatggg cctcggcacc taccgcctct ccgtcgacaa cgggatcacc
     9061 agcccgtacg cgcacctgcg ccagtacgtc gtgtccagca ccctcaagcc ggacaccgac
     9121 ccagccgtca ccgtcgtgcc gagcgacccg cgcgccctca tccgcgagct caaaggcgag
     9181 atggccgctg gcccagacat ctggctctgc gggggcggaa ggctcgcggg caccctgttg
     9241 cccgagattg acgagctgct gatcaagacc tatccggtcg ttgccggtac cggaattttg
     9301 gtagtcgacg gtgccttcga ccccaccgtc ttcaacgtcg ccgaacgcac cgccttcccc
     9361 aacggggtca ccctcacccg cctcacccgc cgctaggcag tttcggttca gcgggcggac
     9421 cagaagaaga tgcccgcgat atggagtccg gccaggtaga tggtcgctgt cttctcgtag
     9481 cgggtggcga taccgcgcca ctgcttgagg cggttgatgc accgctcgac ggcgttgcgc
     9541 tgcttgtagg cgtcgcggtc gaaggccggt ggcctgccgc cttggctgcc gcgccgctgc
     9601 cggtgggcct gctggcccgc cgggacaggg atcactgcgc ggatcccgcg cttgtgaagg
     9661 tgggcgcgga tcgcgcgcga ggagtacgcc ttgtccgcca gaaccacatc tggcctggtg
     9721 cggggccgtc cgcgtcggcg cggaacacgc agacgtgcca tcacctgcgc gaatgcgggt
     9781 gcgtcgccgg cctgcccggc ggtgagatga aaggccaggg gccggcagct gccgtcggcg
     9841 gcgaggtgga tcttcgtggt caatccgccg cgggaccggc cgatggcgtg gtcgtcgggc
     9901 tcgccgaccg gggccccttt ttgcgggccc cggccgcatg ctggtgagcg cgcacgatcg
     9961 tagagtccac cgacacggcc caattgaggt cctcctccgc gtcggcctgg gcgatcagcg
    10021 cggtgaacac ccgctcccag gtgccgtcga cggcccacat ccgcagccgg ttgtagacgc
    10081 ctcgccagtt gccgtacttc tccggcaggt gcacccactg cgttccggtc tggaacttga
    10141 aggcgatcgc gttgatcacc tcacggtgat cccgccagcg gccaccccgc ttcggtgtcc
    10201 ggtcgggaag tagtggctcg atccgcgccc actgcgcatc agtcaacggc acgccgggac
    10261 caacgatccg atgatccaaa cgaaacggcc gttgtgctgt cccgggacgt tggtgacacg
    10321 cgtgctgggt gcttgaacgg gtgagggcct tctgggttcg gtgtggattg cgacatctac
    10381 cgccgaccgc caggaggccc tcgtgtccca ccgtaacgcc ccgctgaccc cgaccggcag
    10441 gctgcgtctg gcccggtgtg tcgtggacga aggctggccg ttgcggcggg ccgcggaacg
    10501 cttccaggtc agccacacca ccgccgcccg ctgggccggc cgctaccgac agctg
//
