LOCUS       NZ_LN831790            11398 bp    DNA     linear   CON 08-APR-2024
DEFINITION  Streptomyces leeuwenhoekii strain type strain (C34 = DSM 42122 =
            NRRL B-24963) chromosome 1.
ACCESSION   NZ_LN831790
VERSION     NZ_LN831790.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces leeuwenhoekii
  ORGANISM  Streptomyces leeuwenhoekii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Gomez-Escribano,P.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2015) JIC, Department of Molecular Microbiology,
            Norwich Research Park, Norwich, NR4 7UH, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001013905.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 03:45:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,096
            CDSs (total)                      :: 7,005
            Genes (coding)                    :: 6,806
            CDSs (with protein)               :: 6,806
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 82 of 199
            Pseudo Genes (incomplete)         :: 160 of 199
            Pseudo Genes (internal stop)      :: 10 of 199
            Pseudo Genes (multiple problems)  :: 49 of 199
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:33:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2013689
            Orig. end                         :: 2025087
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LN831790.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11398
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="16"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11398
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11398
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6398](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6398
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(48..62)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     misc_feature    231..242
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.7"
                     /tool="antismash"
     misc_feature    465..478
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.45"
                     /tool="antismash"
     misc_feature    complement(466..479)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.11"
                     /tool="antismash"
     gene            701..1063
                     /locus_tag="BN2145_RS09455"
                     /old_locus_tag="ABM27_RS09315"
     CDS             701..1063
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BN2145_RS09455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ABM27_RS09315"
                     /product="hypothetical protein"
                     /protein_id="WP_047122429.1"
                     /transl_table=11
                     /translation="MDLANSPNLEEVFGQAPLRPRFYSISGITAATKWWRDELDEETLQ
                     CYLGTSEEHAAPGHMSRMKTVIIGDLGPDLPFVLDYRDSFADPSVAFLGEGDAWRRVSD
                     NVRALLAMLDGQRPDA"
     misc_feature    730..744
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
     gene            1413..1712
                     /locus_tag="BN2145_RS09460"
                     /old_locus_tag="ABM27_RS09320"
     CDS             1413..1712
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BN2145_RS09460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ABM27_RS09320"
                     /product="hypothetical protein"
                     /protein_id="WP_029385269.1"
                     /transl_table=11
                     /translation="MADQQTGSGFSIKMCDLPVTLPAREDSEWSLREILEWAPEHSDWG
                     AKRICVAGCGVTCLEVPEGTLIEVPTEDALDMRLVAPAEVERRLAENRLWRDPE"
     gene            complement(1743..2852)
                     /locus_tag="BN2145_RS37715"
     CDS             complement(1743..2852)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BN2145_RS37715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_029385268.1"
                     /transl_table=11
                     /translation="MGALIEYWAEAVERADTDIEVIAPPYTGIKAGIAWALFMVVPALG
                     ALCVTALRRRARAGRRPAGDLGAEVRRILLYVVLLWLGAQAAGILLYMPFRQVTGEPPT
                     RIVFITPAEDLVFVSRRAASRACVLLLIAGVMAGLRLSGGSSRPARLDFRAPVRVLAGV
                     LPACLGRGLLFGSVLGVVLAFAAPWGPMIAWSTRLVACGFGAFFGLLFGLGLAVLRWAR
                     VPADLEQETPQSTLRGERAAAAALLVFALVPLLAKWFIAFEGHFAGSDIQALDAVGRWL
                     ETLEANVAMGLAAGLLAVSGTAYFAYRGAGLRLAAARRLQSRPLSFLEDARMLSVLRRV
                     GPVYHFCHAEFQDRIVSAAGAAGGAPQRA"
     gene            complement(2789..4435)
                     /locus_tag="BN2145_RS37720"
     CDS             complement(2789..4435)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /locus_tag="BN2145_RS37720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NACHT domain-containing protein"
                     /protein_id="WP_234342280.1"
                     /transl_table=11
                     /translation="MARSRTWWPWAVGAVGVVVLVTVVALVVGAGAQAAATSLSVVISA
                     VAGLLSWSWHRSRPAAPATRAELDQAAEALAAMVRRQWTEEAAAQGLLDPHPLAVRWRS
                     GQSEAGDHVRMVGRTLSGRSDDIPAFAAAFRALPRRRLVIVGEPGAGKTALAILLVREL
                     LRDPEPGEPVPVLLDLASWNPRREPLAPWMARRIHEDYPALRDHETYGRDAARKLVAEG
                     RVLPVLDGLDEIPADLRPEALASVNHAIALHDPLVLTSRRAEYHRLVEEADVITAAAVV
                     VAQPVEAADVVDYLRSAVPPRHIPAWQPVLDDLAGRPRGAVAQALSVPLNVWLARTVYG
                     SPGTDPAELTRWNDAETIRHHLLDALVPAVFTAPPAATDRSVPDVRRTAAARWHPDDAR
                     TALAFLADHVRRQRTDGIAWWDLHRALRPDPAGPLSGPAAGAMIGLGAGCMAGDYFHWA
                     LPPFTRVLCFLAVTLATAVGAGVVIRLAHVGGDPGAPGGRTVPGEGGTSPARSCAGSGS
                     HSPSSCRWLRGWERSSSTGRRRWNARTRTSR"
     misc_feature    4441..4455
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 17.93"
                     /tool="antismash"
     gene            5001..6398
                     /locus_tag="BN2145_RS09470"
                     /old_locus_tag="ABM27_RS09335"
     CDS             5001..6398
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122140.1"
                     /locus_tag="BN2145_RS09470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS09335"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_029385265.1"
                     /sec_met_domain="DUF692 (E-value: 1.9e-98, bitscore: 327.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIADAVERMPGIDWVEVVAENVCPGHLPASLRR
                     LRERGVTVVPHGVSLGLGGAERPDPDQLTALAERARALDAPLVTEHIAFVRAGGPLTAT
                     PRLEAGHLLPVPRTRDALDVLCENVRVAQDALPVPLAVENIAALFSWPGEEMTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNRGEDPAKALAELPLEAIAYVHVAGGFERDGVWHDSH
                     AHPVPPPVLGILAELAARVSPPGVLLERDENFPAPAELEGELEAIRAALRAGRRLATGE
                     HAGETGRVPAGAAHPAHPAHPSRPEGPDGPVPPAGAGEGPVDSPGTGPARERLGLAQAA
                     LLSALVAGTPAPEGFDRVRLRVQTRALAAKRAGVVAKVAPELPEILGDGYRPAFLAYAH
                     GRPMTGGYRQDALDFAERLLGDGLPVDARARRALRQWWLERSGPVPRSRRPAVRLARAT
                     RRVLLRR"
     gene            6505..7299
                     /locus_tag="BN2145_RS09475"
                     /old_locus_tag="ABM27_RS09340"
     CDS             6505..7299
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125238.1"
                     /locus_tag="BN2145_RS09475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS09340"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_029385263.1"
                     /transl_table=11
                     /translation="MFWVLLLLPAWVLAGTACARLCLTAVRTAATDTAGADAGGGRALT
                     LYEAAFLSGGPGRVADLALVTMACRRQLLLAHTGWATVVDPRGRDEMERSVLGAIGPGG
                     QSRIAPVRASAATADAVRGLAEHLVGAGLALPDGGRDSVAAAVRRVRLAALAVCALGAA
                     AVALPVPMGTPRHLIALWFALPLLLTLGCLAVARMEIHPYARWASPAGQRLLCALPRQS
                     GGADGERTFLTSVALRGVHAIGEPDLRAAFAHRERHAGRYGS"
     misc_feature    complement(7385..7398)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.49"
                     /tool="antismash"
     gene            7428..9044
                     /locus_tag="BN2145_RS09480"
                     /old_locus_tag="ABM27_RS09345"
     CDS             7428..9044
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (115-298). E-value:
                     3.7e-15. Score: 48.1. Matches aSDomain:
                     nrpspksdomains_BN2145_RS09480_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034041.1"
                     /locus_tag="BN2145_RS09480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS09345"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_047121666.1"
                     /sec_met_domain="PF00561 (E-value: 2.5e-21, bitscore: 75.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAVVHSAAGALLLTALAAAPAGGAPERPGAAEGPGAAERRGTA
                     VAAARAAAAGISFGPCPDAADLPGALRCGTVTVPLDYARPDGRQIRLTVGRMRATGKDP
                     RGGGRAVPRQGALVYNPGGPGASGLYFPLAGLSPEWKRLAAAYDLVGYAPRGVAPSAPL
                     SCQDPEHFFPGPGPAPQQPSASYKRQRIAQARAYARGCAERSGDALRHHHSLNNARDLD
                     VVRAALGEKRLTFMGASYGTYFGALYAELFPRHVRRMVFDSVVDPHPGRIWYRNNLAQS
                     EAFERRWADFRAWIAAHDGVYGLGRTPGAVQRGYEVASARLSARPAGGTVGPAQLQEML
                     LRAVYYDDQWPHRAHALSAYLKGDPRPLIDLAEPHPEAAAEAENASAVYTAVECNDAPW
                     PTDFRVWDRDNTRLARRAPFETWENVWTNLPCAYWPAPRQRPLEVRTGPGELPPTLLLA
                     GERDGATPYEGALELHRRLAGSVLVTERGTGTHGVAAGPNACVNGHLEAYLLEGRLPAR
                     DAFCAPRPAPEPTSPAKSSAP"
     aSDomain        7773..8321
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BN2145_RS09480_Abhydrolase_1.1"
                     /evalue="3.70E-15"
                     /label="BN2145_RS09480_Abhydrolase_1.1"
                     /locus_tag="BN2145_RS09480"
                     /protein_end="298"
                     /protein_start="115"
                     /score="48.1"
                     /tool="antismash"
                     /translation="ALVYNPGGPGASGLYFPLAGLSPEWKRLAAAYDLVGYAPRGVAPS
                     APLSCQDPEHFFPGPGPAPQQPSASYKRQRIAQARAYARGCAERSGDALRHHHSLNNAR
                     DLDVVRAALGEKRLTFMGASYGTYFGALYAELFPRHVRRMVFDSVVDPHPGRIWYRNNL
                     AQSEAFERRWADFRAWIAAH"
     gene            complement(9045..9776)
                     /gene="hemQ"
                     /locus_tag="BN2145_RS09485"
                     /old_locus_tag="ABM27_RS09350"
     CDS             complement(9045..9776)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="BN2145_RS09485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS09350"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_029385204.1"
                     /transl_table=11
                     /translation="MSDDASTPAPERIPNKGKLAKDLNEVIRYTLWSVFRLKDALPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDVSGLRADADLMIWWHAETSDQLQEAYNLFRRTR
                     LGRALTPVWSNMALHRPAEFNRSHIPAFLADETPRNYVSVYPFVRSYDWYLLPEEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARLHVR
                     EEVPFFTGRRKDIGELVAGLA"
     gene            complement(9781..11244)
                     /gene="hemG"
                     /locus_tag="BN2145_RS09490"
                     /old_locus_tag="ABM27_RS09355"
     CDS             complement(9781..11244)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034034.1"
                     /locus_tag="BN2145_RS09490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABM27_RS09355"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_029385203.1"
                     /sec_met_domain="DAO (E-value: 4.4e-07, bitscore: 28.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREEETPAGTGHVVVIGAGIAGLAAAHRLLERGARVTVLEASGRV
                     GGKLLPGEIAGVRVDLGAESMLARRPEAVGLARAAGLADRLQPPATATASLWTRGALRP
                     MPKGHVMGVPGTADALSGVLSEEGLARIERDAALPRTEIGDDVAVGAYVAERLGREVVD
                     RLVEPLLGGVYAGDAYRISMRSAVPQLFEAARRHASLTEAVRALQGRTATAPPSGPVFM
                     GIRGGIGTLPPAVARSVRARGGDIVTDAPVTELRRTAPTGWRVVAGERVLHADAVVVAV
                     PAPAAARLLRAEAPAAAAELAAVEYASMALVTLAYRRSDAGALPEGSGFLVPPVDGRTI
                     KASTFASRKWGWIAEQDPGLVVLRTSVGRYGETEILGRDDEGLVAVSRHDLKEATGLDA
                     EPVAARVTRWTDGLPQYPVGHHARVARVREHVAALPGLAVCGAAYDGVGIPACVASAYA
                     AVDQIHGDLRAVQELTAHPVQSLHGGAGE"
ORIGIN
        1 ccgcgacggc cggccacccc ttctgggtgc cgtccctgcg ggagtggatc gacgccggtg
       61 agctgaagcc cggacagtgg ctccagacct cctccggcac gtgggtccag gtaacggcga
      121 tcaaggcgtg gaccgcccac aaggccaccg tccacaacct caccgtcacc gacgtccaca
      181 cgtactatgt gctggcgggg tccaggccgg tcctcgtcca caactgcaac gaaaacataa
      241 tctccaacgc gccagggttc gacaattcag cagaactctc ccgcgtctcc gacggggatg
      301 tctacagcgg ggtttacgag ccgagcagcg ggacgttcta tgcccgtctg agtctcgacg
      361 agaaggaccc cgcaagattc ccgaacgccg tcctgtcgaa cggtgggcac ggagaggtca
      421 acttctggca cttcaagggc tcgcgagaga ccatcgggtt caacatgttc ttcgaaaagg
      481 gcggcatatc ggtggcttgg ttctcccgaa gcgtgaacag gaggaatcac ggggatccca
      541 tggcaccttc aggtgcacgg caggcgatca tggacgccat ctccggcgcc acgggcaggc
      601 gggtgtggtc gcggtgaacg gcgacgggga gtcctgtgag ccgattttcg gcatgccgct
      661 ccccgctcgc ctcgtcacag cgatacgaga tcgcagttgg atggatctgg ccaactcccc
      721 gaacctcgag gaggtgttcg gccaggcccc tctgcgaccc cgtttctact cgatctccgg
      781 aatcaccgca gcgacgaagt ggtggcgcga cgagctcgac gaggaaaccc tgcagtgcta
      841 cctcggcaca tccgaagaac atgccgcgcc tgggcatatg tcgcggatga agaccgtgat
      901 catcggggac ctgggtccgg atcttccctt cgtcttggac taccgcgatt ccttcgccga
      961 tccaagcgtt gcctttctgg gggaaggtga tgcctggcga agggtctccg ataatgtccg
     1021 cgccctgctc gccatgctcg acgggcaacg gccggacgcc tagccgtggg ggtgtccgtg
     1081 ggggcgtgag gtgcgacctc ctccctgatc agccaactac cgcaccggat aggcaatagc
     1141 acgtatggag ccccgccagg aattgtcctg gcggggcttc tgcgcgtcgt gacggcagtg
     1201 gctgacggca atgcgtcagc gaaccggggg cggcgcgagg tggcggttca tcgccgtcgc
     1261 cgtgccccgg ccgccgtgct ccggccgggg cacggccgag ccgtgccggg gcgccgtgtc
     1321 gctctccggt ggccggggtg cgggggtggt tccacgttgg ccggagatcc gccctggacg
     1381 ggcggaaacc ggtcgaacga ggaggacgga tcatggcgga ccagcagacc ggcagcggct
     1441 tctcgatcaa gatgtgcgat cttccggtga ccctgccggc cagggaggac agcgagtggt
     1501 ccctgcggga gatcctcgaa tgggctccgg agcactccga ctggggggcg aagaggatat
     1561 gcgtggcggg ttgcggggtg acctgcctgg aggtgcccga ggggacactg atcgaggtgc
     1621 ccaccgagga cgccctggac atgcggctgg tcgccccggc ggaggtcgag cggcggctgg
     1681 cggagaaccg cctgtggcgc gaccctgagt gaaccgcctg tggcgcgacc ccgagtgagg
     1741 gatcacgccc gctggggcgc gccgcccgcc gcgccggccg cgctgacgat gcggtcctgg
     1801 aactccgcgt gacagaagtg gtacacgggc ccgacccgtc tgagcacact gagcatccgg
     1861 gcgtcctcca ggaacgacag gggacgactc tgcagccgtc tggccgcggc gagccgtagc
     1921 cccgccccgc ggtaggcgaa gtaggcggtg cccgacacgg ccagcagacc cgcggcgagc
     1981 cccatcgcca cgttcgcctc cagggtctcc agccagcggc cgacggcgtc gagggcctgg
     2041 atgtcactgc ccgcgaaatg tccctcgaac gcgatgaacc acttggccag gagcggcacc
     2101 agcgcgaaca ccagcagggc ggcggccgcg gcgcgctcac cgcgcaaggt cgactgcggc
     2161 gtttcctgct ccaggtcggc cgggacccgc gcccagcgca gcaccgccag accgaggccg
     2221 aacaggagcc cgaagaaggc gccgaagccg cacgccacga gcctcgtcga ccaggcgatc
     2281 atggggcccc agggggccgc gaaggccagc acgaccccca ggacggagcc gaacagcagc
     2341 ccccggccga ggcaggcggg cagcaccccc gcgagcacgc ggaccggggc gcggaagtcc
     2401 agacgggcgg ggcgggacga accgccggac aggcgcagtc ccgccatgac accggcgatc
     2461 agcagcagga cacaggcacg ggaggcggca cgccggctga cgaagaccag gtcctcggcc
     2521 ggggtgatga agacgatccg ggtggggggc tcgccggtca cctgccggaa cggcatgtac
     2581 aggaggatgc cggcggcctg ggcacccagc cagagaagca cgacgtacag caggattctc
     2641 cgtacctcgg cgccgaggtc gcccgccggg cggcggcccg cccgggcacg ccgccgcagt
     2701 gctgtcacgc acagggcgcc gagggccggc accaccatga acagggccca ggcgatgccc
     2761 gccttgatcc cggtgtacgg cggcgcgatc acctcgatgt ccgtgtccgc gcgttccacc
     2821 gcctccgccc agtactcgat gagcgctccc atccccgcag ccagcggcag gacgacggcg
     2881 agtgcgagcc cgatccggcg caggacctcg ccggtgatgt gccgccctcc cccggcacgg
     2941 tccggccgcc cggggcccct gggtcaccgc cgacatgggc gagccggatc accacgccgg
     3001 cgccgaccgc cgtggcgagc gtgacggcga gaaagcacag gacccgcgtg aacggtggca
     3061 gcgcccagtg gaagtagtcc cccgccatgc accccgcccc cagtccgatc atggcgcccg
     3121 ccgcggggcc ggacaggggg cccgccgggt cggggcgcag ggcgcggtgc aggtcccacc
     3181 aggcgatgcc gtcggtgcgc tggcgtctga cgtggtcggc gaggaaggcg agcgcggtgc
     3241 gggcgtcgtc ggggtgccag cgggcggcgg cggtgcggcg cacgtcgggg accgagcggt
     3301 cggtggcggc cggcggggcg gtgaagacgg ccgggaccag cgcgtcgagg aggtggtggc
     3361 ggatggtctc ggcgtcgttc cagcgggtca gttcggcggg gtcggtgccc ggggagccgt
     3421 agacggtgcg ggcgagccag acgttcagcg gcaccgacag ggcctgcgcg acggcgcccc
     3481 ggggccggcc ggccaggtcg tccaggaccg gctgccaggc cgggatgtgc cggggcggga
     3541 cggcgctgcg cagatagtcg accacgtccg cggcctccac cggctgcgcg acgacgacgg
     3601 cggcggcggt gatcacgtcg gcctcttcga ccagccggtg gtattcggcc cggcgggagg
     3661 tgaggacgag cgggtcgtgc agggcgatgg cgtggttgac ggaggccagc gcctcgggcc
     3721 gcaggtcggc ggggatctcg tccaggccgt ccaggacggg caggacacgt ccctcggcga
     3781 ccagtttgcg tgccgcgtcc cggccatagg tctcgtggtc gcgcagcgcc gggtagtcct
     3841 cgtggatgcg gcgtgccatc cacggtgcca gtggctccct gcgcgggttc cacgacgcca
     3901 ggtccagcag taccgggacg ggctccccgg gttcggggtc gcgcaggagt tccctgacga
     3961 gcaggatcgc cagggccgtc ttgcccgcgc cgggctcgcc gacgatcacc agccgccggc
     4021 gcggcagggc gcggaaggcc gcggcgaagg cggggatgtc gtcgctgcgg ccggacaggg
     4081 tgcggcccac catccgtaca tggtcgccgg cctcgctctg gccgctgcgc cagcgcaccg
     4141 ccagcgggtg cgggtccagc aggccctgcg cggcggcctc ctccgtccac tggcggcgca
     4201 ccatcgcggc gagcgcctcg gcggcctggt ccagctccgc ccgggtggcc ggcgcggcgg
     4261 gccgcgaccg gtgccacgac cagctcagca gccccgcgac cgccgagatc accacgctca
     4321 gcgacgtcgc ggccgcctgg gcgcccgcgc cgaccaccag ggccaccacc gtgaccagca
     4381 ccacgacacc gaccgccccg accgcccacg gccaccacgt cctgctccgt gccatcacgg
     4441 ggatcttccg gtccggggtg gtgcggtgcg tgcggcgccg tgcgcggcgt ggccccccgg
     4501 gggcactgac catggcgaca ctccctcgca ccgtccggcg attcgcgcgg tcggccaccg
     4561 gtgcgggccg ccggggcggt gcgcgtacgg cggcggtggc tcgccaggag ccgaaagcca
     4621 caagccggtg agccgccggc cctccggccg gcccgtcccg ggggccgctc cggcacgcgt
     4681 cgggcgcgac gcacgtgaag gggacggttg atcgatctcg ctcgggaccg cggtgcccgg
     4741 gcgtgcgccg agcgcctacg gccgggtcag ggcccgagcg cgtgcgtggg cacctcgttt
     4801 cagggtggca cagagtgcgc gcgggcgcga tgcggcacag ggcctgcgcc gcgccgccgc
     4861 cgcggaagtg cgggccgggg cgagcgcgga agctcaaatg ttgctctgaa cgtgcccgcg
     4921 ccggggttgg ggcacggagg gccggggcca tacccccctc accgccggga cggcattccg
     4981 gcgtgcggag gagggggacg atggagcgac tggggaccgg tatcgggtgg cggccggaga
     5041 tagcggacgc cgtggagcgc atgcccggca tcgactgggt cgaggtcgtg gccgagaacg
     5101 tctgccccgg ccacctgccc gcctccctgc ggcgtctgcg cgagcgcggt gtcaccgtcg
     5161 taccgcacgg cgtctccctc ggtctcggcg gtgccgaacg gcccgacccg gaccagctga
     5221 ccgcgctggc cgaacgggcc cgggcgctgg acgcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtccgcgc gggcgggccg ctgaccgcca ccccgcgtct ggaggcgggg catctgctgc
     5341 ccgtgccgcg cacccgggac gccctcgacg tgctgtgcga gaacgtccgc gtcgcgcagg
     5401 acgcgctgcc cgtgccgctc gccgtcgaga acatcgccgc cctcttctcc tggccgggcg
     5461 aggagatgac ggaggggcag ttcctgtacg agctggccga ccgcacgggg gtgcgcctgc
     5521 tgatcgacgt ggccaacctc cacaccaacc acgtcaaccg cggcgaggac ccggccaagg
     5581 cgctggccga gctccctctg gaggccatcg cctacgtcca tgtcgcgggc ggcttcgaac
     5641 gcgacggtgt ctggcacgac agccacgccc accccgtccc gccgccggtc ctcggcatcc
     5701 tggccgagct cgccgcgcgc gtctcaccgc cgggcgtgct cctggagcgg gacgagaact
     5761 tccccgcacc ggccgagctg gagggcgagc tggaggcgat ccgggcggcg ctgcgcgcgg
     5821 ggcggcggct cgccaccggg gagcacgccg gggagacggg ccgtgtcccg gccggggcgg
     5881 cccacccggc tcacccggct cacccctccc gcccggaggg cccggacggc ccggtcccgc
     5941 ccgccggggc gggggaaggg cccgtggact cccccggcac cgggcccgcc cgggagcggc
     6001 tcggtctggc gcaggccgcg ctgctgtccg cgctggtggc ggggacgccc gcgccggagg
     6061 ggttcgaccg ggtgcggctg cgggtgcaga cgcgggcgct ggcggcgaag cgggcgggcg
     6121 tcgtggccaa ggtggcgccg gaactgccgg agatcctcgg ggacggctac cggccggcct
     6181 tcctcgccta cgcccacggc cgccccatga ccggcgggta ccggcaggac gcgctcgact
     6241 tcgccgaacg gctgctgggc gatggcctgc cggtggacgc gcgcgcccgg cgcgcgttgc
     6301 ggcagtggtg gctggagcgg tccgggccgg tgccgcgctc gcggcggccc gccgtacggc
     6361 tggcccgggc cactcgccgc gtgctgctgc ggcgctgacc accgcgcagg gcgagaacgg
     6421 aacgccacgt cacataccgg cgacgctgcg tccgcatggt gaaaaggcat ggcgcgcccg
     6481 gcgcccctgg catacaaacg cggcatgttc tgggtccttc tcctgctgcc ggcctgggtc
     6541 ctggccggca cggcctgcgc acggctgtgc ctgaccgcgg tccgcacggc agccacggac
     6601 acggccggcg cggacgcggg cggcggacgc gccctgaccc tctacgaggc ggcgttcctg
     6661 tccggcggcc ccggacgggt cgctgatctg gcactggtga ccatggcgtg ccggcgccaa
     6721 ctgctgctgg cccacaccgg ctgggccacc gtcgtcgacc cgcgcgggcg ggacgagatg
     6781 gagcggtcgg tcctcggggc catcgggccg ggcggccagt cccggatcgc gcccgtgcgg
     6841 gcgtccgccg ccacggcgga cgcggtgcgc gggctggccg agcacctcgt cggcgcggga
     6901 ctggccctgc cggacggcgg ccgcgacagt gtggcggccg ccgtccgccg ggtccggctg
     6961 gccgccctcg ccgtgtgcgc gctgggcgcc gccgccgtgg ccctgcccgt ccccatgggc
     7021 acaccgcggc atctgatcgc cctgtggttc gcgctgccgc tgctgctgac cctgggctgc
     7081 ctcgccgtgg cgcgcatgga gatccatccg tacgcgcggt gggcctctcc cgccgggcag
     7141 cggttgctct gcgcgctccc ccggcagagc ggcggcgcgg acggcgagcg gacgttcctg
     7201 acctccgtgg cgctgcgcgg cgtccacgcg atcggcgaac cggacctgcg ggcggccttc
     7261 gcccaccgtg agcggcacgc cgggcggtac gggagctgag ggcgcgccgg tgagcgccga
     7321 tccgccgcgc ggcgggcaca ccgacgcgcc gcaccgcggt ccttgccttg cccggtgatc
     7381 gacccaaaca tccctttcat cgccgccgtg ctcggaaggg atccgcgatg agagctgccg
     7441 tcgtccactc ggccgcaggg gccctgctcc tgaccgccct cgccgcggcc ccggccggcg
     7501 gcgcaccgga acggccgggc gcggcggagg ggcccggcgc ggccgagcgg cgcggcaccg
     7561 cggtggccgc cgcgcgcgcc gcggcggccg gcatctcctt cggcccctgc ccggacgcgg
     7621 cggacctgcc cggcgccctg cgctgcggca cggtcacggt cccgctcgac tacgcgcgcc
     7681 cggacgggcg gcagatccgg ctcaccgtcg gccgcatgcg ggccacgggg aaggacccgc
     7741 gcggcggcgg gcgcgcggtg ccccggcagg gcgccctggt gtacaacccg ggcgggccgg
     7801 gcgcctccgg gctgtacttc ccgctggccg ggctgtcgcc ggagtggaag cggctggcgg
     7861 ccgcctacga cctcgtcggc tacgccccgc gcggggtcgc cccctccgct cccctgtcct
     7921 gccaggaccc ggagcacttc ttcccggggc ccggcccggc cccgcagcag ccctcggcgt
     7981 cgtacaagcg gcagcgcatc gcccaggcca gggcgtacgc gcgcggctgc gcagagcggt
     8041 ccggcgacgc gctgcgccac caccactccc tcaacaacgc ccgcgacctg gacgtggtgc
     8101 gggcggcgct gggcgagaag cggctgacgt tcatgggtgc ctcgtacggg acgtacttcg
     8161 gggcgctgta cgcggagctg ttcccccggc acgtacgcag gatggtgttc gactcggtgg
     8221 tggacccgca ccccgggcgg atctggtacc gcaacaacct ggcgcagtcg gaggcgttcg
     8281 agcgccgctg ggcggacttc cgggcgtgga tcgccgccca cgacggcgtg tacgggctcg
     8341 ggcgcacacc cggcgcggtg cagcgcggct acgaggtggc gagcgcccgt ctgtcggcgc
     8401 gcccggcggg cggcacggtc ggtccggcgc agttgcagga gatgctgctg cgggccgtgt
     8461 actacgacga tcagtggccg caccgggcgc acgcgctgtc ggcctatctg aagggggacc
     8521 cgcggcccct gatcgacctg gcggagccgc acccggaggc ggcggccgag gcggagaacg
     8581 cgagcgcggt ctacacggcc gtcgagtgca acgacgcccc gtggccgacg gacttccgcg
     8641 tctgggaccg ggacaacacc cggctggcgc gccgggcgcc cttcgagacc tgggagaacg
     8701 tgtggaccaa cctgccctgc gcctactggc cggcgccgcg gcagcggccg ctggaggtgc
     8761 gcaccgggcc gggtgagctg ccgccgacgc tgctgctggc cggggagcgc gacggggcca
     8821 ccccctacga gggggcgctg gaactgcacc gccggctggc gggctcggtg ctggtgaccg
     8881 agcgcggcac gggcacgcac ggtgtcgccg ccgggccgaa cgcctgcgtg aacggccatc
     8941 tggaggcgta tctgctggag ggccggctcc cggcgcggga cgcgttctgc gccccgcgcc
     9001 cggcgccgga gccgacgagc cccgccaaga gctccgcgcc ctgatcaggc gagccccgcc
     9061 accagctccc cgatgtcctt gcggcggccg gtgaagaacg gcacctcttc gcggacgtgc
     9121 aggcgggcct cggaggcgcg caggtgacgc atcaggtcga cgatgcggta cagctcgtcg
     9181 gcctcgaagg cgaggatcca ctcgtagtcg cccagggaga aggaggcgac cgtgttggcg
     9241 cgcacgtccg ggtagccgcg ggccatcttg ccgtggtcgg cgagcatccg gcggcggtcc
     9301 tcctccggca ggaggtacca gtcgtaggag cgcacgaacg ggtagacgct gacgtagttc
     9361 cgcggcgtct cgtcggcgag gaacgccggg atgtgcgagc ggttgaactc ggcggggcgg
     9421 tgcagcgcca tgttcgacca caccggcgtg agcgcgcggc cgagcctggt gcggcggaag
     9481 aggttgtacg cctcctggag ctggtcgctg gtctcggcgt gccaccagat catcaggtcg
     9541 gcgtcggcgc gcaggccgga gacgtcgtag gtgccgcgga tcgtcacgtc cttggcggcg
     9601 agctggtcga acagctcctg gacctcgtcg gcgtacccgg cgcggtcctc cgggagcgcg
     9661 tccttcagcc ggaagacgga ccagagggtg tagcggatga cctcgttgag gtccttggcc
     9721 agcttgccct tgttggggat ccgctcgggg gcgggggtgg aggcgtcgtc actcatgggc
     9781 ctattctccc gctccgccgt gcaggctctg caccgggtgg gcggtgagct cctgcacagc
     9841 gcgcaggtcg ccgtggatct ggtcgacggc cgcgtaggcg ctcgcgacgc aggccgggat
     9901 gccgacgccg tcgtacgccg ccccgcacac ggccagtccc ggcagtgcgg cgacgtgctc
     9961 gcggacgcgg gccacgcgtg cgtggtggcc gaccgggtac tgcggcagcc cgtcggtcca
    10021 gcgggtgacg cgcgcggcga ccggttcggc gtccaggccg gtggcctcct tcaggtcgtg
    10081 ccgggagacg gccacgaggc cctcgtcgtc ccggccgagg atctccgtct cgccgtaccg
    10141 gccgacggag gtgcgcagca ccaccaggcc ggggtcctgc tcagcgatcc agccccactt
    10201 gcgggaggcg aaggtggagg ccttgatggt ccgcccgtcg accgggggca cgaggaatcc
    10261 gctgccctcg ggcagcgccc ccgcgtcgga gcggcggtag gcgagggtga ccagtgccat
    10321 ggaggcgtac tcgacggcgg cgagctccgc cgccgcggcg ggcgcctcgg cccgcagcag
    10381 gcgggcggcc gcgggcgccg ggacggcgac gacgaccgca tcggcgtgca ggacgcgctc
    10441 cccggcgacg acccgccatc cggtgggcgc ggtgcggcgc agttcggtca ccggcgcgtc
    10501 cgtgacgatg tcgccgccgc gggcccgcac cgatcgggcg accgccggcg ggagcgtgcc
    10561 gatgccgccc cggatgccca tgaagaccgg tccgctcggc ggggcggtgg ccgtccggcc
    10621 ctgcagggcg cggaccgcct cggtgaggga ggcgtgtctc cgggcggcct cgaagagctg
    10681 cgggacggcc gagcgcatgg agatgcggta ggcgtcgccc gcgtacacgc cgccgagcag
    10741 cggttcgacg aggcggtcga cgacctcgcg gcccagccgc tcggccacgt aggcgcccac
    10801 cgccacgtcg tcgccgatct ccgtgcgggg cagggcggcg tcgcgctcga tgcgggcgag
    10861 cccctcctcg gacagcacgc cggacagggc gtcggccgtg ccggggacgc ccatgacgtg
    10921 ccccttgggc atggggcgca gcgccccccg ggtccacagg gaggccgtcg cggtggccgg
    10981 cggctggagg cggtcggcga gacccgccgc acgggccagg cccaccgcct cggggcggcg
    11041 ggcgagcatg gactcggcgc cgaggtcgac ccgcaccccc gcgatctcgc cgggcagcag
    11101 cttgccgccg acccggccgg acgcctccag caccgtcacc cgcgcgccgc gctccagcag
    11161 ccggtgggcg gcggccagcc ccgcgatgcc ggccccgatg acgaccacat gccccgtacc
    11221 cgcaggggtc tcctcttcgc gcatgtcccc agcctctcag acggcaccga cagcgccgcc
    11281 cggcccccgg gcgtccggcg cgagtcccga ccgtgaccac ttcggaaccg ttccgcgcca
    11341 acgtcccggc cgccccgggc gtcgaaggga cgtcagatct caccaccccg gggggaac
//
