LOCUS       NZ_LDCN01000002        23571 bp    DNA     linear   CON 11-DEC-2024
DEFINITION  Brevibacillus formosus strain DSM 9885 Scaffold2, whole genome
            shotgun sequence.
ACCESSION   NZ_LDCN01000002
VERSION     NZ_LDCN01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Brevibacillus formosus
  ORGANISM  Brevibacillus formosus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Paenibacillaceae; Brevibacillus.
REFERENCE   1  (bases 1 to 997053)
  AUTHORS   Wang,J.P., Liu,B., Liu,G.H., Chen,Q.Q., Zhu,Y.J., Chen,Z. and
            Che,J.M.
  TITLE     Genome Sequence of Brevibacillus formosus F12T for a
            Genome-Sequencing Project for Genomic Taxonomy and Phylogenomics of
            Bacillus-Like Bacteria
  JOURNAL   Genome Announc 3 (4), e00753-15 (2015)
   PUBMED   26205874
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997053)
  AUTHORS   Liu,B., Wang,J., Zhu,Y., Liu,G., Chen,Q., Chen,Z., Lan,J., Che,J.,
            Ge,C., Shi,H., Pan,Z. and Liu,X.
  TITLE     Genome sequencing project for genomic taxonomy and phylogenomics of
            Bacillus-like bacteria
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 997053)
  AUTHORS   Liu,B., Wang,J., Zhu,Y., Liu,G., Chen,Q., Chen,Z., Lan,J., Che,J.,
            Ge,C., Shi,H., Pan,Z. and Liu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2015) Agricultural Biological Resources Research
            Institute, Fujian Academy of Agricultural Sciences, 247 Wusi Road,
            Fuzhou, Fujian 350003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. May-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001012775.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 11:34:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,848
            CDSs (total)                      :: 5,688
            Genes (coding)                    :: 5,626
            CDSs (with protein)               :: 5,626
            Genes (RNA)                       :: 160
            rRNAs                             :: 1, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 146
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 27 of 62
            Pseudo Genes (incomplete)         :: 43 of 62
            Pseudo Genes (internal stop)      :: 9 of 62
            Pseudo Genes (multiple problems)  :: 14 of 62
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:55:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 609733
            Orig. end                         :: 633304
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LDCN01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..23571
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="azole-containing-RiPP"
                     /region_number="2"
                     /rules="((YcaO or TIGR03604) and (((thio_amide and (PF06968
                     or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N
                     or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or
                     PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or
                     thiostrepton or TIGR03602)"
                     /tool="antismash"
     cand_cluster    1..23571
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="((YcaO or TIGR03604) and (((thio_amide
                     and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /kind="single"
                     /product="azole-containing-RiPP"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..23571
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:13571](+)"
                     /cutoff="10000"
                     /detection_rule="((YcaO or TIGR03604) and (((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /neighbourhood="10000"
                     /product="azole-containing-RiPP"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..13571
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="((YcaO or TIGR03604) and (((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /neighbourhood="10000"
                     /product="azole-containing-RiPP"
                     /protocluster_number="1"
     gene            192..1109
                     /db_xref="GeneID:87585020"
                     /gene="cyoE"
                     /locus_tag="AA984_RS07985"
                     /old_locus_tag="AA984_07990"
     CDS             192..1109
                     /EC_number="2.5.1.141"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585020"
                     /gene="cyoE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248322.1"
                     /locus_tag="AA984_RS07985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_07990"
                     /product="heme o synthase"
                     /protein_id="WP_047069199.1"
                     /transl_table=11
                     /translation="MQREESVATRNRPMSNILAQTVKTGIIKSNLIPMFAGLTLALYTY
                     HFSLLDKLPEILFATIGSILIMGAAGAFNNVYDRDIDSIMERTKNRPTVTGDISTKTVL
                     WLASLMTIFGLVALVLATPLAAIMGFLGLFFYVVPYTMWTKRRTVYNTEVGSISGAMPP
                     LIGWAAIHPDITHPAILGLFIIMVIWQMPHFYAIAIRKHDEYKAAGVPMLPVVKGARRT
                     YIQTNVYLVILIASSFLFWSLSAGLTFMALLLNVLWLVLSVFGYRKMDSQKWSKTMFIY
                     SLIHMTVLFSTVIIYSLMGIIFKL"
     gene            1274..1984
                     /db_xref="GeneID:87585021"
                     /locus_tag="AA984_RS07990"
                     /old_locus_tag="AA984_07995"
     CDS             1274..1984
                     /codon_start=1
                     /db_xref="GeneID:87585021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371087.1"
                     /locus_tag="AA984_RS07990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_07995"
                     /product="carboxylesterase"
                     /protein_id="WP_047069200.1"
                     /transl_table=11
                     /translation="MIRHNLLIGNIPAILWGDKSDKLFIVVHGNMSNKADDSIIVFAEE
                     ATAVGYQVLSFDLPQHGERKDDTYLCKVQNCVQDLNKIMTYAKSLSNHISIFACSMGVY
                     FSLLEYCHEPLNQCLFLSPVVNMERIIDNMMTWFNVSESRLKIEKEISTPIGQTLYWDY
                     YCYVKEHPIVAWNNPTSILYGSGDNLCEFDIVSEFAKRFNCNLQVMENGEHYFHTPEQL
                     QYFRQWLKKNIYVK"
     misc_feature    complement(2014..2030)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 23.71"
                     /tool="antismash"
     misc_feature    2056..2067
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 19.35"
                     /tool="antismash"
     misc_feature    complement(2103..2118)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.95"
                     /tool="antismash"
     misc_feature    2106..2121
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: strong, score: 26.61"
                     /tool="antismash"
     misc_feature    2108..2122
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.0"
                     /tool="antismash"
     misc_feature    2109..2122
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.06"
                     /tool="antismash"
     misc_feature    2147..2165
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.83"
                     /tool="antismash"
     gene            2149..3774
                     /db_xref="GeneID:87585022"
                     /gene="nikA"
                     /locus_tag="AA984_RS07995"
                     /old_locus_tag="AA984_08000"
     CDS             2149..3774
                     /EC_number="7.2.2.-"
                     /GO_component="GO:0009897 - external side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA]"
                     /GO_function="GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /GO_process="GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585022"
                     /gene="nikA"
                     /gene_functions="transport (smcogs) SMCOG1068:
                     extracellular solute-binding protein family 5"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016885502.1"
                     /locus_tag="AA984_RS07995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08000"
                     /product="nickel ABC transporter substrate-binding protein"
                     /protein_id="WP_047069201.1"
                     /transl_table=11
                     /translation="MFGSTKKAVLLMVAVILLLSTTLLGCTKKETESNGENKAASAEKV
                     ITLSWPRDVGPMNPHTYNPSQLFAQSMIYEPLVSYKEGGKLEPYLAESWSISKDGKEYT
                     FKLRQNVKFSDGTPFNAAIVKKNFDAVMKNPKLHSWLGVINALDKTEVVDEQTFKMTLK
                     EPYYPALQDLALVRPVRFLGEAGFPDDGDTSKGLKKPIGTGPWVLAEYKKDEYAVFTRN
                     PNYWGESPKIDKVTVKIIPDAETRVMAFEKGELDLIYGEGAISMDSFNQLKESGKYVTE
                     LSDPVGTRSLLLNTSNEKLADLRVRMALQHGFNKQAMVEGITLGLEEKADNILSKNFPY
                     TNMDVQQVEYNVEKANAYLDEAGWKLPAGKTVREKDGQPLELELIYDKTDPIQKAMAET
                     MQAEWGAIGVKLNITGLELTVQIKRRKAGEFDLDFWYNYGAPYDPHSFINVVAEKGWGV
                     SEAHSHLSMKQELDQQIKETLASTDEAKREQLYNSILRTLQDQSVIVPVSYIKKTVVYQ
                     KNVKDFSFPANRDEHPFYGISISK"
     gene            3793..4737
                     /db_xref="GeneID:87585023"
                     /gene="nikB"
                     /locus_tag="AA984_RS08000"
                     /old_locus_tag="AA984_08005"
     CDS             3793..4737
                     /EC_number="7.2.2.11"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585023"
                     /gene="nikB"
                     /gene_functions="transport (smcogs) SMCOG1118:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013373739.1"
                     /locus_tag="AA984_RS08000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08005"
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="WP_047069202.1"
                     /transl_table=11
                     /translation="MVSYIGKRILAIIPIVLFAILITFVLIHISPVDPAEAYLSAAHIH
                     PTEELLAEKRHEFGLDQPLIVQYFQTVMKICRLDFGRSYLTNEPVWGEVTARMPATIQL
                     AVSSILFAMIVSIPLGFLSAVYKNSLIDNFSRLLSFFGASIPQFWLGYLLIFFFSVKLD
                     WLPVEGRGSWEHLVLPTMTLSLALIAIYTRLLRASVLEQLQESYVLYARTRGIKEKVIM
                     VKHVLKIAISPVITGLGMNFGKLLTGTIIVEQVFSWPGFGRYFVEAIFNRDIPVIQCYV
                     LLAACLFIVCNLIVDLVQLYMDPRISLKGRTDQ"
     gene            4734..5567
                     /db_xref="GeneID:87585024"
                     /gene="nikC"
                     /locus_tag="AA984_RS08005"
                     /old_locus_tag="AA984_08010"
     CDS             4734..5567
                     /EC_number="7.2.2.11"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585024"
                     /gene="nikC"
                     /gene_functions="transport (smcogs) SMCOG1085:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013082615.1"
                     /locus_tag="AA984_RS08005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08010"
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="WP_047069204.1"
                     /transl_table=11
                     /translation="MIPHIRTVLKGQKTITVCSVLVLVFAIITVLAPWISPHDPIKVNV
                     AMKLKPPSAEHLLGTDHLGRDNLSRLLHGARISLGFAFLIFVSSIGIGLIVGTIAGYKG
                     GWIDTVLMRFCEGVMAFPNLVLVLGIVGVFGPGLIQVVLALMLVQWVYYARMFRSMVVS
                     LKERNFIIAARISGSSQWKIIKQHMIPNILPPILVMGTLEMGWAIMDISALSFLGLGIQ
                     PPTPEWGAMIHEGKSFIRSHPELMLYPGLMILFVVITFNVLGEALSERFGVKRRF"
     gene            5586..6410
                     /db_xref="GeneID:87585025"
                     /locus_tag="AA984_RS08010"
                     /old_locus_tag="AA984_08015"
     CDS             5586..6410
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585025"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016822609.1"
                     /locus_tag="AA984_RS08010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08015"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_047069205.1"
                     /transl_table=11
                     /translation="MEEPRKVLQVSGLHVKVKAEQGMLSLIQDIHFDLKSGQVLGLVGE
                     SGSGKTVTCNSLLQLLDRKNTIVEGSIQLNGRELNGLKAEEMRRIRGKEIAFIMQNPMS
                     AFTPVYTIGMQFIETIRTHTMLTKKQAIELAVTSMEDVNLPDPPKLMNLYPFQLSGGML
                     QRVMIAISMCLRPSVVIADEPTTALDVVNQLQVLRQLDRLRLEYGTSIMLISHDLGVIS
                     ELADEVAVMQHGQIVEQANVFKLFDHPQHAYTKKLLNARPAFPLQQLNRESV"
     gene            6473..7291
                     /db_xref="GeneID:87585026"
                     /gene="nikE"
                     /locus_tag="AA984_RS08015"
                     /old_locus_tag="AA984_08020"
     CDS             6473..7291
                     /EC_number="7.2.2.11"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585026"
                     /gene="nikE"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017149829.1"
                     /locus_tag="AA984_RS08015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08020"
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="WP_047069206.1"
                     /transl_table=11
                     /translation="MLLQVKDVTHSYGVPRLFWRSDRRVPVLSDISLTIEQGMCLGLLG
                     TSGAGKSTLGKVILGLEKPRQGQVFFQGHDLYSTDIQTRKVLRRDLQVVFQDCYSSVNP
                     RMTAERIIGEPLDNYERLSLNEQKRKIGELLEMVGLNPEDMRKYPNQFSGGQLQRINIA
                     RAIALKPKMIVLDESVSSLDMVNQTHILGLLRELKSVFGLSYLFITHDIKAAYSICDGL
                     AVLEKGELVEMFDDKDRIFASSHATVRSLISSILSEHPRHRSLSLDGDPT"
     misc_feature    7563..7576
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.21"
                     /tool="antismash"
     gene            7641..7895
                     /db_xref="GeneID:87589091"
                     /locus_tag="AA984_RS29170"
     CDS             7641..7895
                     /codon_start=1
                     /db_xref="GeneID:87589091"
                     /inference="COORDINATES: protein motif:HMM:TIGR03601.1"
                     /locus_tag="AA984_RS29170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_083995042.1"
                     /transl_table=11
                     /translation="MDEFKKELKKLKASEIKADQVQRWDKQKMNEATLVRGRCGCGRCG
                     CGRCGCGRCGRCSCSCSCSCSCSCFFPCSCTCFFPCFIV"
     aSDomain        8082..8324
                     /aSDomain="Thiopeptide_F_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing F protein in a thiopeptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_AA984_RS08025_Thiopeptide_F_RRE.1"
                     /evalue="1.30E-13"
                     /identifier="RREFam004.1"
                     /label="AA984_RS08025"
                     /locus_tag="AA984_RS08025"
                     /protein_end="81"
                     /protein_start="0"
                     /score="41.7"
                     /tool="antismash"
                     /translation="MRIKVKRDTVYLPDSDGSVYFRNNIGTFRMKGDMIDRWVDQLMPM
                     FNGEYTLAQLTDDLPEEYQQQIYQVAGTLLQNGFLQ"
     gene            8082..10004
                     /db_xref="GeneID:87585027"
                     /locus_tag="AA984_RS08025"
                     /old_locus_tag="AA984_08030"
     CDS             8082..10004
                     /GO_process="GO:0030152 - bacteriocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585027"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thiopeptide_F_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03693"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Heterocycloanthracin_C_RRE"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717950.1"
                     /locus_tag="AA984_RS08025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08030"
                     /product="putative thiazole-containing bacteriocin
                     maturation protein"
                     /protein_id="WP_236697850.1"
                     /sec_met_domain="Thiopeptide_F_RRE (E-value: 4e-12,
                     bitscore: 41.7, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03693 (E-value: 2.9e-176, bitscore:
                     583.0, seeds: 2, tool: rule-based-clusters)"
                     /sec_met_domain="Heterocycloanthracin_C_RRE (E-value:
                     2.6e-38, bitscore: 125.6, seeds: 5, tool:
                     rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIKVKRDTVYLPDSDGSVYFRNNIGTFRMKGDMIDRWVDQLMPM
                     FNGEYTLAQLTDDLPEEYQQQIYQVAGTLLQNGFLQDMSLEKPHELSPEIMSAYGEQIE
                     FLDQFGGSGGYLFQKYRTEKVLIVGAGSFLLSVVHALLESGCVEFHYVITNEAPTNRER
                     LDAIIEHTRRKDPHVKLGELKPASWGIAEWSEAIEPFAAILYTSAQVNKDEVSVIHSAC
                     RGRKKVFVPAVCLMQKGIAGPLIHPESAACLESAWRRLHRQTLCADPDQHPFSMTAAAI
                     LANIAVFEWFKHVTGVNEAEDENKVYVLNLETLEGGWRSYLPHPLVNKEMSLEVLDRKE
                     LESRLSNMTEPKEGLQSLFQSWTSSDTGIFHSWEEGELTQLPLTQCMVTAVDPVSEGPA
                     ELMPQVVCPGYTHEEARREAGLLGAELYVGRMVNHLATIDTAFSAVGNGNIGVGAGETP
                     AEGLNRALRHCLTLTYVADMKGRQPTARPVHADTIADEPCQFYIRSFTTLGEKPTIAKG
                     YGVYGFPVYWVGTRTGWYGSIGLQERDALRSALMYALQQIQTPTTQVSKYVLTAQSVGL
                     AGEAIEVALNSVQEESEKEVWKQAVEVLTLHHLSPLVINAAMEPFLEEDLGGVFALLLS
                     QEVPR"
     aSDomain        8085..8357
                     /aSDomain="Heterocycloanthracin_C_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing C protein in a
                     heterocycloanthracin LAP cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_AA984_RS08025_Heterocycloanthracin_C_
                     RRE.1"
                     /evalue="8.30E-40"
                     /identifier="RREFam011.1"
                     /label="AA984_RS08025"
                     /locus_tag="AA984_RS08025"
                     /protein_end="92"
                     /protein_start="1"
                     /score="125.6"
                     /tool="antismash"
                     /translation="RIKVKRDTVYLPDSDGSVYFRNNIGTFRMKGDMIDRWVDQLMPMF
                     NGEYTLAQLTDDLPEEYQQQIYQVAGTLLQNGFLQDMSLEKPHELS"
     gene            10001..11941
                     /db_xref="GeneID:87585028"
                     /locus_tag="AA984_RS08030"
                     /old_locus_tag="AA984_08035"
     CDS             10001..11941
                     /codon_start=1
                     /db_xref="GeneID:87585028"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: TIGR03882"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016740499.1"
                     /locus_tag="AA984_RS08030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08035"
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="WP_047069210.1"
                     /sec_met_domain="TIGR03882 (E-value: 6e-49, bitscore:
                     162.1, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 1.6e-76, bitscore: 253.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 7.2e-124, bitscore:
                     409.1, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSADILIIGDGVLAELVSERLRKDYHVWRSENMESATSVADLALL
                     LHDTYHPALHLQAEKNFRQANIPWLRGFVSFGEAIVGPLVRPGQTGCSQCADMRRLMAG
                     RDRKEMWKVQHMLSEKGGILRDPWASRSGLLQAAYHVSTEVDRIFEGRQAQLSEHIQFI
                     DLQTLTSTRHFFLPNPLCPVCSQLPEDSEEQARIRLKPIIKQSLDSYRTKSIADLKQAL
                     STEFLDSRTGFLNGKARDLVSVFADVSVNLPLFSHDEGTAGRSVSYADSEITAILEGME
                     RYCGLMPRGKRTMVRDCYANLASHALDPTTAGTHSEEQYALPDFPFQPFDPKREMNWVW
                     GYSFKEERPILVPELMAYYSMGFGEGVVFETSNGCALGGSLEEAIFYGILEVVERDAFL
                     LTWYAQLPLPRLDPTTVDDRELLLLIDRMQTVTDYDLYLFDATMENGIPAVWTIAKNRR
                     AEGLHFVCAAGAHPDPIRAVKSSIFETAAMLMPLTDKYEANRDKYLRMLQDSSLVKKMD
                     DHAMLYSLPEAAERLSFLMDDSRPMVSFAEQFPKRNQNRDLTDDLRELLGVFQKLDLDV
                     IVVDQTTPEVGRHGLSCVRVLIPGMLPMTFGHHLTRVTGLKRVLEVPVKLGYANQPLRV
                     DELNPFPHPFP"
     gene            11964..13571
                     /db_xref="GeneID:87585029"
                     /locus_tag="AA984_RS08035"
                     /old_locus_tag="AA984_08040"
     CDS             11964..13571
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585029"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: PF00881"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248326.1"
                     /locus_tag="AA984_RS08035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08040"
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="WP_047069211.1"
                     /sec_met_domain="PF00881 (E-value: 1.5e-06, bitscore: 24.1,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLENFLYRLHFDIENARSSDLEVDWGDAPLTYKVYRGLPVIPLP
                     EDVPLSLVDQTVSQEMSLPQLGAWLWYSYGLIQISQSLLTTTSADAESESFLPLVTNRR
                     PVPSGGGLYPSELYVYLKLSSIPPGLYHYDVAHHRLVQLHEGNFDHVLSRALGGSCPVH
                     ACFGVAFVSIYFWKNFFKYDEFSYRLQGLDAGVLLGQLQEMANQFGVTTAVHYQYLDRA
                     INALLGLSADQESVYAVVPLSLQAEGERHSHSPAPWTERGEETASELIRALPEQKHVHV
                     MRSKRMRQYPALLQMNEAAAQESTHAMRRYSDHFESKETRRFDERILLPRLEKPTYDLA
                     QYCRERFSPEMDFVRKKTNQNQVARIVEMTMSALSYGNDLGNNQEAAWEMLSLLVSIHQ
                     VEGLQDGAYAYEPMEHALYPIVYGDVRARLQDGMTLDNVNLYQVPLCFHVAGKRDHLIN
                     QLGFRGYRIQQMEAGIVVHHLLLAAFTCGMGGHPLLGFDVENCDDLYNLETEEKTCLIQ
                     IPVGHYRPRARFQTGLHG"
     gene            complement(13612..15357)
                     /db_xref="GeneID:87585030"
                     /locus_tag="AA984_RS08040"
                     /old_locus_tag="AA984_08045"
     CDS             complement(13612..15357)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_5 (197-571). E-value: 9.2e-66.
                     Score: 214.1. Matches aSDomain:
                     nrpspksdomains_AA984_RS08040_Aminotran_5.1"
                     /codon_start=1
                     /db_xref="GeneID:87585030"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /locus_tag="AA984_RS08040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08045"
                     /product="cysteine desulfurase-like protein"
                     /protein_id="WP_083995043.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1e-63, bitscore:
                     211.2, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 3.9e-06, bitscore:
                     22.0, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.9e-09,
                     bitscore: 33.0, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTTLNTELVRIQPWADDDLPLLRLLNAPAMMEQLGGPETEEQLL
                     ARHERYVNKDGQLTSKMFSIVLLPDNTPVGSIGYWDRIWQGENVYEVGWSVLPPFQGKG
                     IASAALALLITRINQERRHKFIHAYPSVDNPASNAICRKLGFTLQSECVFEYPPGSLMR
                     SNDWRLEVASGHFPIEKVREQFPALQRTYKDKPVVYLDGPGGSQVVKSSMDAIYRYMAN
                     GGANLHGSFPTSKETEAILADAKQAVADLFHVRPLEVAFGANMTTLTFAIARALGRHWK
                     EGDEIIVSEMDHRANVDPWLTVAADRGMTVRWLKVDKETLTLQLDELDTLLTSRTRLVA
                     VGLASNAVGTIHDLAAISQKARAKGVVVAVDAVHAVPHFSIHRDEIGADILLCSAYKFF
                     GPHVGIAVIRKEIFEALDVYKLVPAPSSYPDKLETGTQNHEGIAGIRPAIEFVASLGLG
                     DNFPDKIRSGYEVIEAYENQLAEKLRKELGAMKGVTIYQAGEDVPKTPTIAFRIEGFSP
                     QEICERLCEEHSIFVADGDFYATTLANGLGINESGGWIRAGLAPYNTREEIDRFIQGIK
                     AIIRA"
     aSDomain        complement(13645..14766)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AA984_RS08040_Aminotran_5.1"
                     /evalue="9.20E-66"
                     /label="AA984_RS08040_Aminotran_5.1"
                     /locus_tag="AA984_RS08040"
                     /protein_end="571"
                     /protein_start="197"
                     /score="214.1"
                     /tool="antismash"
                     /translation="VYLDGPGGSQVVKSSMDAIYRYMANGGANLHGSFPTSKETEAILA
                     DAKQAVADLFHVRPLEVAFGANMTTLTFAIARALGRHWKEGDEIIVSEMDHRANVDPWL
                     TVAADRGMTVRWLKVDKETLTLQLDELDTLLTSRTRLVAVGLASNAVGTIHDLAAISQK
                     ARAKGVVVAVDAVHAVPHFSIHRDEIGADILLCSAYKFFGPHVGIAVIRKEIFEALDVY
                     KLVPAPSSYPDKLETGTQNHEGIAGIRPAIEFVASLGLGDNFPDKIRSGYEVIEAYENQ
                     LAEKLRKELGAMKGVTIYQAGEDVPKTPTIAFRIEGFSPQEICERLCEEHSIFVADGDF
                     YATTLANGLGINESGGWIRAGLAPYNTREEIDRF"
     gene            15606..16118
                     /db_xref="GeneID:87585031"
                     /locus_tag="AA984_RS08045"
                     /old_locus_tag="AA984_08050"
     CDS             15606..16118
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015891003.1"
                     /locus_tag="AA984_RS08045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08050"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_047069212.1"
                     /transl_table=11
                     /translation="MNEKSIHTQRLILIPFTHRIATNILQEDYEELLDMGLTLGEGWPD
                     EDAIETIPKIIKALELTGEPTGFESWMIITKAGRKIIGDAGFKGVPNAEGEVDIGYGII
                     EAERKKGYAYEAAEGLANWALLQPDVKKITAKCLLDNIDSAKLLVKMNFTEIKRDDTMI
                     YWSRQKE"
     gene            complement(16193..17017)
                     /db_xref="GeneID:87585032"
                     /locus_tag="AA984_RS08050"
                     /old_locus_tag="AA984_08055"
     CDS             complement(16193..17017)
                     /codon_start=1
                     /db_xref="GeneID:87585032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016740503.1"
                     /locus_tag="AA984_RS08050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08055"
                     /product="hypothetical protein"
                     /protein_id="WP_047069213.1"
                     /transl_table=11
                     /translation="MFRKIVHSLVCFLLVITAGAVNVNAAQPKATGMFFIDKPFKKEGL
                     RFQVTVTEIDNPKYDSPTYHVNTIVTNISRKPITYTTGHCYGVTTRLEAYASGDSWELP
                     YPESEPACPTVIQEKTLKPGRTIEQDEYFRTDELPDGLTPDSFELNSSVRILVGPEDER
                     KEEFVKMQTKLLPGLSLNPDEIELQAESSVLPKSYYELKVTGKASNNDIKRVTIQVFNT
                     VITLRIDSKSGEFSTKKKVKAKGPSLQSGVLEVTYNDGTKHTYNVPIENSSN"
     gene            17272..17883
                     /db_xref="GeneID:87585033"
                     /locus_tag="AA984_RS08055"
                     /old_locus_tag="AA984_08060"
     CDS             17272..17883
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585033"
                     /gene_functions="other (smcogs) SMCOG1188: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717970.1"
                     /locus_tag="AA984_RS08055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08060"
                     /product="DedA family protein"
                     /protein_id="WP_047069214.1"
                     /transl_table=11
                     /translation="MQNWMTQIIEQYSYFGILLMMALENIFPPIPSEVILTFGGFMTTQ
                     SSLTVFGVIMSATIGSVIGAIILYGIGYYLDVKKIEKIVARWGHILRIKPADIHKANEW
                     FDRYGYWTIFFCRMVPLIRSLISIPAGMTKMNFPLFLLFTTLGTFIWNIVLVMAGHLLG
                     ESWEDILYYFDLYSNVVYVVLAFAGVAFLIFFLRNRKADA"
     misc_feature    17960..17976
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.12"
                     /tool="antismash"
     gene            18068..19552
                     /db_xref="GeneID:87585034"
                     /locus_tag="AA984_RS08060"
                     /old_locus_tag="AA984_08065"
     CDS             18068..19552
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585034"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248331.1"
                     /locus_tag="AA984_RS08060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08065"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_174769352.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.3e-33, bitscore:
                     112.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDLLLVNGVVITMDKDRRVLQDGAVAIDKGRILEVGDTPLLKDK
                     YPAQKVVDCAHHCILPGFVDAHGHGGHSLFKTIATDNINDWMPIMTNTYKHYVTDDFWY
                     YEGKLSALERLKAGITTGVSVLGSMPRSDDPIFALNHAKAYNEVGVREIVATGPCNPPW
                     PHPFSRWVDGQKVTKEVTYEQVLAGAEAVIEALNHANHDKTRAYITPFVIVTSVNPSYP
                     TPADQLFGLTDFDRYQARKIREIAKKYDTRIHSDAFGGMIHLAIQDKEYALLGPDVHLQ
                     HCRGISFDEAKILAETGTNVSASPGFGQINARTPITELLEMGATVAITTDGTSPMTPFD
                     MFQAMRRMQLLQQAALRDYYYLPPGKLLEMVTIDAARCVGWDDELGSLEAGKKADVITV
                     NMHQAHLNPENMHVHRVVYQAVGGDVNHVIVDGELIMDERRVLTVDEKQILKEANEEAN
                     RTIERAGLETYMQPSKYFWGHARAYLDERRFDPTKL"
     misc_feature    complement(19631..19640)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     gene            19737..20579
                     /db_xref="GeneID:87585035"
                     /locus_tag="AA984_RS08065"
                     /old_locus_tag="AA984_08070"
     CDS             19737..20579
                     /EC_number="7.6.2.14"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585035"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717977.1"
                     /locus_tag="AA984_RS08065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08070"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_047069215.1"
                     /transl_table=11
                     /translation="MYLTIDNVTKHFVNEQKQKVKVLDDINLEVEKGSFVSIVGPSGCG
                     KSTLLYLIAGLDQPDSGDIHVAGNKVNKPGPDRVVVFQEAGLFPWLTVLENVTYGLKLK
                     KMSTAKANEKALEVLKMVHLSKYVHSYPHQLSGGMKQRVAIARALVMEPDILLMDEPFS
                     ALDEQTRMVLHKELLEIWRTTKVTIFFVTHNIREAVQLAERIVVFATRPGKIKEIIAVP
                     AMRDGVMPDSVTVNTEQKVLSILQEEIEKVLKEEMGNDYSFKTGHLHRYDSGDLGSHI"
     gene            20512..21267
                     /db_xref="GeneID:87585036"
                     /locus_tag="AA984_RS08070"
                     /old_locus_tag="AA984_08075"
     CDS             20512..21267
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585036"
                     /gene_functions="transport (smcogs) SMCOG1044: ABC
                     transporter, permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372021.1"
                     /locus_tag="AA984_RS08070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08075"
                     /product="ABC transporter permease"
                     /protein_id="WP_047069216.1"
                     /transl_table=11
                     /translation="MTTALRRVIFIAMIAGIWEVTSKLSGLPSFMFPSLTQVFETLVNG
                     LISGQITAAIGKSMGRILLGFLIAIIVGLILGYFIWRYKLVEDTLGFVVTALQSIPSIV
                     WFPLAIIWFGLNDFSILFIVTIGATWTMTVNATSGFKNVPPLYQRVARTYGSTGFHFVR
                     TVILPASVPQIISGLRIAWAFSWRALMAGELLGGGGGLGQLLEMGRSLGQMDLVISVMI
                     IIAIIGTIVDNVVFSRIERNVQMKWGVHS"
     gene            21296..22291
                     /db_xref="GeneID:87585037"
                     /locus_tag="AA984_RS08075"
                     /old_locus_tag="AA984_08080"
     CDS             21296..22291
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0042918 - alkanesulfonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717982.1"
                     /locus_tag="AA984_RS08075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08080"
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="WP_047069217.1"
                     /transl_table=11
                     /translation="MLKKLFSTVVASLLLMGVASGCSTGGAGSSEKEIKIGYFPNLTHS
                     ATIIALEKGYFKEAFGADMKIQTKTVTNGGLFMEAMATKAIDVGTVGPGPLLNFYVKHP
                     GYRLISGAVNGGAVLVMNGSTTITELKDVKGKKIAIPVIGSTQDVMLRKALNEVGLKPT
                     TNGGDVELYAAAPADTAALFVQKSVDGAATQEPWGYVLENQAGGKLLLDWDQFAWGKES
                     TNTVVAASDEFLKREGLATAYLQAHKKAVKFIQENPEESQDLIIKHLKSLTGKELSKKE
                     VQAAFSRLEVTTAVNEKVIQEMADISQEAGYISSNKIDGLIDLKYLEAAK"
     gene            22372..23232
                     /db_xref="GeneID:87585038"
                     /gene="kynA"
                     /locus_tag="AA984_RS08080"
                     /old_locus_tag="AA984_08085"
     CDS             22372..23232
                     /EC_number="1.13.11.11"
                     /GO_function="GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:87585038"
                     /gene="kynA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015891010.1"
                     /locus_tag="AA984_RS08080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AA984_08085"
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="WP_047069218.1"
                     /transl_table=11
                     /translation="MRPHEQGSNQPNDTSLETQIHTDFQKEMTYGDYLQLDQILSSQQR
                     QSTHHDEMLFIIIHQVSELWMKQILHELSAANECICNHDLEPAFKMFARVSRIQQQLIK
                     SWDVLSTLTPADYLQFRDKLGHSSGFQSYQNRLIEFTLGYKNQHVLAVYAHQPELHAQM
                     SAALQQPSIYDAAIREMAARGLPIDPECLNRDWSQPYQPNQSVEQAWLTVYRNVNQYWD
                     LYELAEKLVDIASQQQQWRFNHMTTVERIIGQKPGTGGSSGVMYLRRALDHRFFPELWS
                     LRTLL"
     misc_feature    complement(23360..23373)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.65"
                     /tool="antismash"
     misc_feature    23360..23373
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.31"
                     /tool="antismash"
ORIGIN
        1 cgtctgttca ttcgtccgcc tccttctctt gacaatcata ccatatagaa ttaacattcc
       61 atatagtatt taaatgctta tcggtatgta agatacaaat ggcagttttg taggatttaa
      121 ccgtctacta tgtcatgcta ttttggagta cgatagatat agatcatcta gtaagagggg
      181 acagtgtacg aatgcagagg gaagaaagcg tagcaacgcg gaatcggcct atgtccaata
      241 ttttggcgca aacggtaaag acggggatta tcaaatcgaa tctgattccg atgtttgccg
      301 gattaacatt ggctttatat acgtatcact tcagtctttt agataagcta ccagaaatct
      361 tatttgctac aatcggctct attttaatca tgggtgcagc gggtgccttt aataacgtgt
      421 atgatcgcga tatcgattcg atcatggagc ggacgaagaa cagacctacg gtgacagggg
      481 atatatcaac gaagactgtt ttatggctcg cctccttgat gacgattttt ggcttggtgg
      541 ctcttgtctt ggcaacaccg ttggctgcta tcatgggttt tctcggactg ttcttttatg
      601 tcgtgcctta tacgatgtgg accaaaagaa ggacggttta caacacggag gtagggagta
      661 tttctggcgc gatgccgccg ctcatcggtt gggctgccat tcacccagat atcacgcatc
      721 ctgctatcct tggtctcttt atcatcatgg tcatttggca aatgcctcac ttctatgcga
      781 ttgcgattcg caagcatgat gagtacaagg cggctggggt tccgatgttg cctgtggtca
      841 aaggtgcaag acgtacctac attcaaacaa atgtttattt ggtcatttta attgccagca
      901 gttttctatt ttggtcatta agcgctggtc ttacatttat ggccctgctc ctgaatgttt
      961 tatggctggt tctgagtgtt tttggatacc ggaaaatgga ttcacagaaa tggtctaaaa
     1021 caatgtttat ttattcgctg attcatatga cggtgctgtt ctcgacggtg attatttact
     1081 cgctgatggg gattattttc aagttgtaga gatgaataga ttaagtatgt cgaaatatgt
     1141 atataattat cgattgatgt agatttggaa aatattataa tataaaagta agatgacccg
     1201 ataagtatct catcggtgtc attttttttt gcttgaaaag agtaagaaca cattttcatg
     1261 gaggatagac gtaatgataa ggcataattt attgatagga aatattccag caattttgtg
     1321 gggggacaaa tcagataagt tatttatagt tgtgcatggt aacatgtcaa ataaggcaga
     1381 tgactcgatt attgtatttg cagaagaagc gacagcagta ggttatcaag ttcttagttt
     1441 tgatttgcct caacatggtg aacgtaaaga tgatacttat ctttgtaaag ttcaaaactg
     1501 tgttcaagac cttaataaaa ttatgacata tgcaaaatcg ttatcaaatc atataagcat
     1561 ttttgcttgt agcatgggag tatatttcag cctattagaa tattgccatg agccattgaa
     1621 tcagtgtttg tttctctctc ctgtggtaaa tatggaacgt attatagata atatgatgac
     1681 atggtttaac gtaagtgaaa gtagactaaa aattgagaaa gaaatttcca cacctattgg
     1741 acaaactctc tattgggatt actactgtta tgtgaaagaa caccctattg tagcttggaa
     1801 taatccgact tccattctct atggttcagg agataactta tgtgagtttg acattgtatc
     1861 tgaatttgct aaacgcttta actgtaattt gcaagtaatg gagaatggag aacactattt
     1921 ccatactccg gaacaattgc agtatttcag acaatggtta aaaaagaata tatacgttaa
     1981 atgattaagg gaagtcaaaa tggcttccct taaaagttat ataatttcaa cattatttta
     2041 agtcaatcga actatgaaaa tgttttctgt tgacataaaa tgtgcccttc tctattataa
     2101 acgtaaatga taatcattat taacatataa gaatggagat accactttat gtttggatca
     2161 actaagaagg ccgttttatt gatggtagct gttattctac tcttatcaac aactctattg
     2221 gggtgcacga aaaaggaaac agagtcaaat ggtgaaaaca aggcggcttc agcggaaaag
     2281 gtaatcacat tatcttggcc gagggatgtc ggaccgatga atcctcatac atataaccct
     2341 tcacaattat ttgctcaatc gatgatctat gaaccgttgg ttagctataa ggaagggggc
     2401 aaacttgaac cctatcttgc agaatcatgg tcgatctcga aggatggcaa agagtatacc
     2461 ttcaagctcc gtcaaaatgt aaaattttcg gacggaaccc cgtttaacgc agctattgtg
     2521 aaaaaaaact ttgatgctgt aatgaagaac ccaaaattgc atagttggct tggcgttatt
     2581 aatgctcttg acaagacaga ggtagtggac gagcaaactt tcaaaatgac tttgaaggaa
     2641 ccctattacc ctgcattgca ggatttggcg ctagtccgtc cggttcgatt cctgggtgaa
     2701 gcgggttttc cggatgacgg cgacacttcc aagggcttaa aaaagccgat tgggaccggc
     2761 ccgtgggtgc tggccgaata caagaaagac gaatatgctg tgtttacccg aaacccaaat
     2821 tactggggcg agagtccaaa gattgataaa gtaaccgtaa agatcattcc cgatgcagag
     2881 acgcgcgtaa tggcttttga aaaaggtgag ttggatttga tatatggtga aggagccatc
     2941 agcatggatt cctttaatca actgaaagaa tcgggaaaat atgtgacgga gttatccgac
     3001 cctgtcggaa cgagaagctt gcttttgaat acctccaatg aaaagctagc tgacctgagg
     3061 gtacgcatgg ccctccagca cggatttaac aagcaagcaa tggtggaagg catcacgcta
     3121 ggtctggagg agaaggcgga caacatcttg tccaaaaact tcccgtatac caatatggat
     3181 gtccaacagg tcgaatacaa tgtggagaaa gcgaatgctt atcttgatga agccggatgg
     3241 aagcttcccg caggcaaaac tgtacgggag aaggacggtc aaccgcttga gttggaattg
     3301 atctatgata agaccgaccc cattcaaaaa gcgatggcgg aaaccatgca agccgaatgg
     3361 ggagcgattg gtgttaaact taacattacg ggtcttgaat tgacggttca aattaaacgc
     3421 cggaaagcag gcgagtttga cttggatttc tggtacaact acggagcgcc atatgatccg
     3481 cactcgttca ttaatgttgt ggcagaaaaa ggatggggcg tttcggaagc acattcgcac
     3541 ttgtctatga aacaagaatt agatcaacaa ataaaagaaa cccttgcctc aacggatgag
     3601 gcaaagcgcg aacagcttta caactccatt ttgagaactt tgcaggacca gtcagttatc
     3661 gttccggtgt cttacatcaa gaaaacggtt gtttaccaga aaaatgtgaa agactttagc
     3721 ttcccggcga atagggatga acatccattc tacgggatta gtatcagcaa gtaatcagca
     3781 gggaggcaac tcatggtcag ctatatcggg aagagaattc tcgctatcat tccaattgtt
     3841 ctctttgcca ttcttatcac gtttgtactc atccatatct caccggtgga cccggctgag
     3901 gcttatttaa gcgcagccca cattcatccg actgaggagt tattggctga gaaaagacat
     3961 gaatttggct tggatcagcc tttgatcgta caatattttc aaactgtcat gaagatttgc
     4021 cgacttgatt tcggcagatc ttatcttacg aatgagccgg tttggggtga ggttacggcc
     4081 cggatgcctg cgaccattca actcgccgtc agcagcattt tgttcgccat gattgttagt
     4141 ataccgctcg gttttttgtc agcggtttat aaaaacagcc tgattgataa tttcagcagg
     4201 ctgctctctt ttttcggcgc ttccattccg caattttggc ttgggtactt attgattttt
     4261 ttcttttccg tcaaactgga ctggcttcct gtagaaggaa gagggtcgtg ggagcatcta
     4321 gtgcttccga ccatgacgtt gtcgttggca ctgatcgcga tctatacccg tcttctgcgc
     4381 gcgagtgtgt tggaacaatt gcaggagtcg tatgtcttat atgccaggac tcggggcatt
     4441 aaagaaaaag tcattatggt gaagcatgtg ttgaaaattg ctatttcacc ggtgattacc
     4501 ggcttgggca tgaatttcgg caaattactc acgggaacca ttatcgtgga gcaagttttt
     4561 tcctggccgg ggttcggacg ctattttgtc gaggcgattt ttaatcggga tattcctgtt
     4621 atccaatgct acgtactcct cgcagcctgt ttgttcatcg tgtgcaacct gattgttgat
     4681 cttgtccagc tgtatatgga tccccgaatt tctttgaaag gaagaaccga ccagtgattc
     4741 cgcatatacg gaccgttctc aagggacaga aaaccataac agtatgctcg gtcttagtac
     4801 tggttttcgc cattattacc gttttggctc catggatatc acctcatgat ccaattaaag
     4861 tgaatgtagc gatgaagttg aagccccctt ccgctgagca tttgctgggt acagaccatt
     4921 tgggacgtga caatctttct cgtcttctgc atggagcccg catatcgctg gggtttgcct
     4981 tcttgatttt cgtttcctcg ataggaatag gcttgatcgt cggaaccatt gccggttaca
     5041 aaggcggttg gattgataca gtactgatgc ggttttgcga aggggtcatg gcgtttccga
     5101 atcttgtgct ggtattgggg attgtcggag tgtttgggcc tggactgata caggtggttt
     5161 tggcgttgat gctggtgcaa tgggtatact atgcccggat gttccgaagc atggtcgtca
     5221 gcttgaagga acgcaatttt attatcgcag ctcgaattag cggctcttcg caatggaaaa
     5281 ttatcaagca gcacatgatc cccaatatac tgcctccgat tctcgtgatg ggcacattgg
     5341 aaatgggatg ggcgatcatg gacatctctg cgctgtcgtt tttgggcttg ggcattcagc
     5401 ctccaacacc tgaatggggg gcaatgatcc acgaaggaaa aagctttata cgaagtcatc
     5461 cggaattaat gctgtatccg ggactgatga ttttgtttgt cgtgattaca ttcaatgtac
     5521 taggggaagc tttgtcggaa cgatttgggg taaaacgacg tttttgaatg ggaatgaggc
     5581 cagtgatgga agaaccacgc aaagtattgc aggtgagcgg tctgcatgtc aaggtaaagg
     5641 cagagcaggg aatgctttct ctgattcagg atattcattt tgatttgaaa agcggtcaag
     5701 tgctcggcct tgtgggggag agcggcagcg ggaagactgt cacctgcaac tccctcctgc
     5761 aacttctgga caggaaaaat accatcgtag aaggcagtat tcaattgaat ggccgagaac
     5821 tgaacggctt gaaagcagag gagatgcgac gtatacgcgg gaaagaaatc gccttcatca
     5881 tgcaaaatcc gatgagtgcc tttacaccgg tgtatacgat aggtatgcaa ttcattgaaa
     5941 cgattcgtac ccataccatg ctaacaaaaa aacaagcaat cgagctggcg gtcacttcca
     6001 tggaagatgt gaatttgcct gatccaccca agctgatgaa cttgtacccc tttcaattga
     6061 gcgggggaat gctacaacgg gtgatgatag ccatttccat gtgcttgcga ccatctgtcg
     6121 tgattgccga tgagccgact acagcgctcg acgtggtgaa tcaattgcaa gtgttgaggc
     6181 agctggaccg attgcgtctg gaatacggca catcgattat gctgatttcc catgatttgg
     6241 gtgtcatttc ggaattggct gacgaagtcg cagtgatgca gcatggacag atcgtagagc
     6301 aggcgaatgt ctttaagttg ttcgatcatc ctcagcatgc gtatacgaaa aaactgctga
     6361 atgccagacc agcctttcct ttacaacagt tgaataggga atctgtttaa ttattttctc
     6421 ctgcatggtt ttactgtcta ttctatgaaa aacggaaaca gggggtgagc gcatgctgct
     6481 tcaggtaaaa gacgtaaccc attcttatgg cgtaccgagg ctgttttggc ggtcagatcg
     6541 acgagtgccc gtcctctccg acatttctct tacgattgaa caaggaatgt gtctgggtct
     6601 actgggaacc agcggtgcag gaaaaagtac attgggcaaa gtgattctgg gattggaaaa
     6661 gcctcgacag ggacaggttt tttttcaagg tcacgatctg tatagcacgg atatacaaac
     6721 tcgtaaagtg ttgcgacggg atcttcaagt cgtgtttcaa gattgctact catcggtaaa
     6781 tccacggatg acggccgaac ggattatcgg agaaccgtta gataactatg agagattgtc
     6841 cttgaacgag caaaaacgaa aaatcggaga gttgctggaa atggtcgggt taaatccaga
     6901 ggacatgcgg aaatacccta accaatttag cggaggacag ctccaaagaa ttaatatcgc
     6961 gcgggcaatt gccttgaaac ctaaaatgat tgtattggat gaatcggtaa gtagcttgga
     7021 catggtgaat caaacccaca ttttaggctt gttaagagaa ttaaaatcgg tattcgggct
     7081 ttcataccta ttcatcacgc acgatataaa agctgcctac tctatttgtg atggactcgc
     7141 ggtactcgaa aaaggggaat tggtagagat gttcgacgat aaggatcgaa tttttgcctc
     7201 ctcccatgcc actgtgagaa gtctgatttc atccattctt tccgagcatc cccgacaccg
     7261 ttctctttct ttagacggag atccaactta acatactgaa aagaatgagg taaataattg
     7321 actgaaacca tatatctacc gtatcggcaa ggtaatactt taaaaaactt taggcaaaca
     7381 tcgctttaaa gtttttttta tttgggctta tttagatggc ctattacaaa ggtgttcatt
     7441 atgactgcac atactcttgg tgagcgagta aaaatgcttt gaacatggca ttaaaggaca
     7501 aatgttaaat ttgcttcaca tatcccgttc attgcataaa tagtaagcgc cttaaaaatg
     7561 ggcgcatgat catggaacaa atacccaatg tcgcttcata aatctagagt agctccatga
     7621 agtggcggag gtgaagatga atggatgaat tcaaaaaaga gctaaaaaaa ctaaaggctt
     7681 ccgaaataaa agcagatcaa gtacagcgct gggataaaca aaaaatgaat gaagcgacac
     7741 ttgttcgcgg gcgatgcgga tgtggtagat gtggatgcgg ccgatgtggt tgtggccgct
     7801 gcgggcgttg tagttgttct tgctcttgct cctgtagctg ttcctgcttc ttcccatgca
     7861 gctgcacttg cttcttccca tgctttatcg tatgaatggc gatattttcg aacgataaac
     7921 cgaatgaagc gccctgccag gatgacgtct tggcaggggt tttgtttttc ccgctggcat
     7981 gtgagacaac gtattctaca taaaataacg atgatacatc atgtcgcaaa agctgatctc
     8041 gcataataag ggggaggaga atggttaggc tgaatccttc aatgcgcata aaagtaaagc
     8101 gggatacggt ttatctgcct gattcagatg ggagtgtata ctttcggaac aacatcggaa
     8161 catttcgaat gaagggggat atgattgacc gatgggttga ccagctcatg ccgatgttta
     8221 atggggagta cacgctggcc caattaaccg acgatttgcc ggaagaatac caacaacaaa
     8281 tataccaggt ggcgggaacg cttttacaaa atggatttct tcaagacatg agtctagaga
     8341 agccgcatga gctttcgcct gaaatcatgt cggcctatgg agaacagatt gaatttttgg
     8401 accagtttgg cggatcgggt ggatatttat ttcaaaaata tcgtacggaa aaagtattga
     8461 tagttggggc aggttccttt cttctttccg ttgtccatgc cttgctggaa tcaggctgcg
     8521 ttgaattcca ttacgtcatc acgaatgaag cacccacgaa tcgtgagcga ttagacgcaa
     8581 tcatcgagca tacacgcagg aaagacccgc acgtgaagct gggggagctg aaaccagcat
     8641 cgtgggggat tgcagagtgg tcggaggcta ttgagccttt cgctgctatt ttgtacacct
     8701 cagcgcaggt aaacaaggat gaagtgagcg tcattcattc cgcgtgcaga gggaggaaga
     8761 aagtatttgt tcctgccgtt tgtctgatgc aaaaaggaat agcaggacct ttgattcatc
     8821 ccgaatcagc agcttgttta gaatcggctt ggcgccgttt gcaccgacag actttatgtg
     8881 cggatcccga tcaacatcca ttttcaatga cggcagcagc aatcctggcc aatattgccg
     8941 tgttcgaatg gtttaaacat gtgactggtg tcaatgaagc tgaggatgaa aacaaggtgt
     9001 atgtactgaa tctggaaaca ctggaaggcg gctggcgatc atacctgccc catccgctgg
     9061 tgaataaaga gatgagcctg gaggtattgg atagaaagga gctcgaatct cggctatcca
     9121 atatgacgga accaaaggaa ggactacagt ctctgtttca gtcgtggacc tcatcggata
     9181 caggaatttt tcacagctgg gaggaaggcg agctcacgca attaccgctc acacagtgta
     9241 tggttacagc cgtcgatcct gtctcagaag gcccggcgga gctgatgccg caagtcgtct
     9301 gccctggtta tacccatgaa gaggcacgaa gagaagcggg acttcttggg gcagaactgt
     9361 acgttggtag aatggtgaac cacctcgcaa ccatagacac tgccttttca gctgtaggaa
     9421 atggcaatat cggagtcggt gcaggggaaa cgccggctga aggacttaat cgcgcactgc
     9481 ggcattgctt gacgctcaca tatgtagcag atatgaaagg gagacaacca acagcgagac
     9541 ctgtccacgc tgacacgatt gctgatgaac catgccaatt ttatatccgg tcatttacta
     9601 cattgggtga aaaaccaacg attgcaaagg gctatggggt gtacggattt ccggtgtatt
     9661 gggtgggaac acgcacggga tggtacggaa gcattggctt gcaggagcga gacgcattac
     9721 gatcagcgct gatgtatgcc ttgcaacaga ttcagactcc aaccactcaa gtgtccaagt
     9781 atgtcctgac agcgcaatct gttggattgg caggtgaagc catcgaggtt gcactcaaca
     9841 gcgttcagga agaatcggaa aaagaagtct ggaaacaggc tgtcgaggtg ctgacccttc
     9901 atcacctttc tccgctggtc atcaatgccg cgatggagcc tttcctggaa gaagaccttg
     9961 gcggagtgtt cgccttgctg ctctcgcagg aggtgcctcg atgagcgctg acattcttat
    10021 cattggagac ggagtactgg cagagctggt ttcggaacgt ttgaggaagg actatcatgt
    10081 gtggcgcagt gaaaacatgg agtctgcgac aagcgtagca gatttggctc tccttttgca
    10141 cgatacttat catcccgctt tgcatttgca ggcagaaaaa aactttcgac aagctaacat
    10201 tccatggctg cgcggttttg tttcatttgg ggaggccatc gtaggtccgt tggttcgtcc
    10261 aggtcaaaca gggtgctccc agtgtgcaga tatgcggcga ctgatggcgg gacgcgatcg
    10321 caaagagatg tggaaggtac aacacatgct gagtgaaaaa ggcggcatac tgcgtgaccc
    10381 gtgggcatcc cgctctggat tgttacaggc ggcataccat gtaagcacgg aggttgatcg
    10441 tatttttgaa ggcaggcaag cccagttgtc agagcatatc caattcattg atttacaaac
    10501 attgacaagc acgcgtcact tctttttgcc gaatccgtta tgtccggtat gcagtcagct
    10561 gcccgaggat tccgaagagc aggcgagaat tcgcttgaag cctatcataa agcaaagtct
    10621 agacagctat cggaccaaat ccatcgccga tttaaaacaa gctctctcca ccgaattttt
    10681 agattcccgt actggatttt tgaatgggaa agcacgtgat cttgtctctg tgttcgcaga
    10741 tgtaagtgtc aatctgccgt tattttccca tgatgagggt acggccggca ggtctgtttc
    10801 ctacgcggat agtgagataa cggcgatttt ggaaggaatg gaaagatatt gcgggctgat
    10861 gccgcgtgga aagaggacga tggtacgcga ctgctatgcc aaccttgctt cgcatgccct
    10921 tgatccgacg acggcgggaa cccattcaga agagcagtat gccctgcctg attttccgtt
    10981 tcagccgttt gatcccaaac gggaaatgaa ctgggtatgg gggtattctt ttaaagaaga
    11041 gcgacccatt cttgtacccg aactcatggc ctactacagt atgggattcg gggagggagt
    11101 tgtatttgag acctccaatg gctgtgcatt aggcggcagt ttggaggagg cgatctttta
    11161 cggcatcctc gaagtggtgg agcgagatgc tttcttgttg acctggtatg cccaattgcc
    11221 attgccacgc ctcgatccga ctacggtcga tgatcgggaa ttgcttctgt tgatcgatcg
    11281 gatgcagacc gttaccgatt acgatttgta cttgttcgat gcgaccatgg aaaatggaat
    11341 acctgctgta tggacgattg cgaaaaatcg gagagcggaa ggcctgcact tcgtttgtgc
    11401 agcaggagcg catcctgacc ccatccgcgc tgtaaaaagc tccattttcg aaacggctgc
    11461 tatgctcatg cctttgacag acaaatacga agcgaatcga gacaaatact tgcgcatgct
    11521 tcaagattcg tccttggtga aaaagatgga cgatcatgcg atgctttaca gcttgccgga
    11581 agcagcggag cgcttatcct ttctcatgga tgattcacgt ccaatggtct ctttcgctga
    11641 acaattccct aaacgaaacc aaaatcgtga tctgacggac gatttgcggg agttgctcgg
    11701 tgtgttccaa aagttagatc tcgatgtcat tgtggtggat caaacgacac cagaggtagg
    11761 gcggcacggc ttatcttgcg tgcgcgtatt gattcctggc atgctcccga tgacttttgg
    11821 ccatcatctc acgcgagtaa cggggttgaa aagagtattg gaggtccctg tgaaactggg
    11881 ttacgcgaat caaccgctgc gtgtagacga gttgaatcct tttccgcatc cgtttccata
    11941 acatgcatct gggagtgagc cggatgagcc tcgaaaattt cttgtaccgc cttcattttg
    12001 atatagaaaa cgccaggtca agtgatcttg aagtggattg gggggatgcg ccactgacat
    12061 acaaagtgta caggggacta cctgtcattc cactgccaga agatgttcct ttatcactcg
    12121 tagatcaaac ggtttcacaa gagatgtctc ttcctcaatt gggcgcttgg ctatggtata
    12181 gctacggact gatccaaatc agccaatcgt tgttaaccac gacctcggca gatgcagaat
    12241 ctgagtcgtt tttgccgctt gtaaccaatc ggagaccggt tccgtcaggt ggaggactct
    12301 acccgagtga gctgtatgtg tatttgaagc tgtcgagtat tccgccaggt ctataccact
    12361 acgatgtggc ccatcataga ctcgtccaat tgcacgaagg gaattttgac catgtcctta
    12421 gccgagcact tggtggcagt tgtccagtac atgcatgctt tggagttgcg ttcgtatcca
    12481 tctatttttg gaagaacttt tttaagtacg atgagttttc ctatcgattg cagggcctcg
    12541 atgctggtgt actgctggga caattgcagg aaatggcaaa ccagttcggt gtgacgaccg
    12601 cggtacatta ccagtacctc gatagagcga taaatgcact gctaggattg tctgccgatc
    12661 aagagagtgt atacgcggta gttcccttat cacttcaagc agaaggggag aggcattcgc
    12721 actcaccggc accatggaca gaacgaggag aggagacggc aagcgagcta atcagagcac
    12781 tgcctgagca aaagcatgtg catgtgatgc gctcaaaaag aatgaggcaa tatcctgcgc
    12841 tgctgcaaat gaatgaagct gccgcgcagg aatctaccca tgcaatgaga cgatattcgg
    12901 atcactttga aagcaaggag actcgtcgtt tcgatgagcg aatcctactc ccgcgtttgg
    12961 agaaaccgac ctatgatctg gcgcaatact gtcgggagcg tttttcacca gaaatggact
    13021 ttgtcagaaa gaaaacaaac cagaatcagg tagcgcgtat cgtggaaatg acgatgagtg
    13081 ctctatcgta cggcaatgat ttgggaaata atcaagaagc agcgtgggaa atgctttctc
    13141 tccttgtaag catccatcag gtagaagggc tacaagatgg ggcatatgcc tacgaaccaa
    13201 tggaacacgc tctttatccg attgtctatg gggatgtgcg agcacgcctg caagacggaa
    13261 tgaccctgga caatgtgaat ttgtatcaag tgccgctatg ctttcatgtg gcgggtaaac
    13321 gcgatcattt gatcaatcag ctagggttta gaggatatcg catccaacag atggaggcgg
    13381 gaatcgtcgt gcatcacttg cttttggcag cgttcacgtg tggaatgggc ggtcacccgc
    13441 tactcggatt tgacgtggag aattgtgatg atctttacaa cctggagacc gaagagaaaa
    13501 cctgcttgat tcaaattccg gtcgggcact atcgcccccg cgccagattt cagaccgggc
    13561 ttcatggata aaaacgtaaa aggcccgagt cgcgttgacc cgggtcttgt attatgctcg
    13621 tataatggct ttgatacctt gaatgaaacg atcaatttcc tcgcgggtat tgtacggtgc
    13681 cagccccgct cgtatccagc cgccactttc attgattccc aaaccgttgg cgagtgttgt
    13741 cgcatagaag tccccgtccg caacgaatat gctatgctcc tcacacaatc gctcacatat
    13801 ttcttgggga gaaaaccctt caattcgaaa agcgatcgtc ggtgttttcg gtacatcttc
    13861 acccgcttga tagatggtga cacccttcat tgcgcctagt tcttttcgca gtttttctgc
    13921 cagctgattt tcataagctt cgatcacctc gtaaccagaa cggattttgt ctgggaagtt
    13981 atcaccgaga ccaagcgacg caacaaattc gatagcgggt ctgattcctg cgatcccttc
    14041 atgattctgt gttccggtct ccagtttatc tggataagaa gagggggcag gaaccaattt
    14101 gtaaacatcg agtgcttcaa aaatttcttt gcggatgaca gcgattccta catgtgggcc
    14161 aaaaaacttg taggcggagc agaggaggat atcggctccg atctcgtcac gatggataga
    14221 gaagtgaggg accgcatgca cggcgtcgac agcaacaacg actccttttg cgcgcgcttt
    14281 ttgggaaatg gcagcgagat catggatcgt tccgacagca ttggaagcaa gcccgactgc
    14341 cacaagtctg gttcgggaag taaggagggt atcgagttca tctagttgta acgtaagtgt
    14401 ctctttatcc accttcaacc accgaacggt cattccgcga tcagcggcaa cagtcagcca
    14461 cgggtctaca ttggcgcggt gatccatctc ggagacgatg atctcatccc cttccttcca
    14521 atgtcgacct aatgcacgcg caatcgcaaa ggtcagggtg gtcatattcg caccgaaggc
    14581 tacctctagc ggcctcacat ggaagaggtc tgctacggct tgcttggcat ctgccaggat
    14641 ggcttctgtc tccttgctcg ttggaaagga gccgtgtaaa ttcgctccac cgttcgccat
    14701 atagcgatag atcgcgtcca tggaggattt tacgacttgt gagccgcctg ggccatcgag
    14761 ataaacaact ggcttgtctt tgtacgttcg ttgtagtgcg gggaattgct cccggacttt
    14821 ttcaatgggg aaatgtccgg atgcaacctc taatcgccaa tcattggagc gcattaaact
    14881 tccaggaggg tattcaaaga cgcattctga ttgcagagtg aaaccaagct tacggcaaat
    14941 ggcattcgag gcagggttgt ccacggaggg ataggcatga ataaacttat gtcttcgttc
    15001 ttggttgatg cgtgtgatga gtaaggctaa agccgcagag gcaattcctt tcccttgaaa
    15061 ggggggcagg acgctccaac ctacttcgta tacgttttct ccttgccaga tgcggtccca
    15121 atagccgatg cttccgacag gggtgttatc tggcagcagt acgatgctga acatcttgct
    15181 cgtaagctgt ccgtccttat tgacgtatcg ttcgtgccga gcaaggagct gctcctccgt
    15241 ctctgggcct ccaagctgct ccatcatggc tggggcattc aaaagacgca gcaagggaag
    15301 atcgtcatcc gcccagggct gaatgcggac aagctcagtg ttaagtgttg tggacatgtt
    15361 ctcactcctt accaactgga tttccgtggt gtccatcctc attcgttatc tctcattatt
    15421 cggaaaagat tccaattaat caatcttcca ttcgttctaa gaaaaaggga tggaaaatca
    15481 tgctataatg gtggtgcccg agtgattctt cctatcggag tattgaaaga gtagctgagg
    15541 tgtaggaata gaccctttct gaaaaaaaca ccgacaataa agaaaagagg aaggggtacc
    15601 tatccgtgaa tgaaaaaagt attcatacac aacggcttat attgattcca tttacacata
    15661 gaatagcgac gaatattttg caagaagatt acgaagagtt attggatatg gggcttacat
    15721 taggcgaagg ctggcctgat gaggatgcga ttgaaacgat acccaaaatc atcaaagcac
    15781 tcgaactcac aggggaaccg acaggatttg aatcgtggat gattattaca aaggctggca
    15841 ggaagatcat cggggatgca gggttcaaag gagtaccgaa tgcggaggga gaagtagaca
    15901 tcggctatgg aattattgaa gccgagcgaa aaaaaggcta cgcgtacgag gcggctgaag
    15961 gcttagcgaa ttgggcattg ttgcagccag atgtgaaaaa gattacggcg aagtgcttgc
    16021 tagataacat agattcggca aaattattag tcaaaatgaa ctttaccgaa ataaagaggg
    16081 acgacacaat gatttactgg tctaggcaga aggaatagca cggaagaaca gcagcagggt
    16141 ttcacacgaa aaaaggacgc acacatggcg gtcctttttt tcgtggagaa catcagttac
    16201 tggaattttc tatgggaacg ttgtaggtat gcttcgtgcc atcgttatac gtaacctcga
    16261 gtacacctga ttgaagggac gggccttttg cttttacttt tttcttcgtg gagaactctc
    16321 ctgacttcga gtcgattcgc aacgtaatga ccgtattaaa gacctgaatc gttacgcgtt
    16381 tgatatcgtt attggaggct ttccctgtca ctttgagttc gtaatacgat ttagggagta
    16441 cacttgattc agcctggagc tcgatctcat ctggattgag ggatagccct ggaagaagtt
    16501 tcgtctgcat ctttacgaat tcctctttac gttcatcttc gggtccaacg aggatgcgta
    16561 cggaagaatt caattcaaaa gagtctggcg taagaccatc cggcagttcg tctgttcgga
    16621 aatattcatc ttgctcgata gttctcccgg gctttaaggt tttttcctgg atgaccgtgg
    16681 gacatgcagg ttcactttca gggtaaggaa gctcccaaga gtctccagag gcatacgctt
    16741 ctagtcgtgt tgttacacca tagcaatgac cagtcgtgta tgtgattggt ttccgcgaaa
    16801 tattggtgac aatcgtattt acgtggtacg ttggtgaatc atattttgga ttgtcgattt
    16861 cggtaacggt cacttgaaac cgaagtcctt ctttcttgaa tggtttgtca atgaaaaaca
    16921 tgccggttgc ttttggttgc gcagcgttta cattcacggc gccagccgtt ataaccagta
    16981 gaaaacatac gagtgaatgg acaatcttgc gaaacacaca gttcacctcc tcacgattca
    17041 tttttaaaaa atgtgcatgg aggcaccttt ttaatattcc atatattccc tccctttgca
    17101 atgttttttg ttcagaaata tttaaaatga aaattttcaa aggaaaggta acccaagact
    17161 tccttctttc gtattgaaat aaaggaaaag atgatcgctt ttcattgaac tatgcaacgc
    17221 gtgtctctat actggagaga taagcattgg taaataattg tgagggatag catgcagaac
    17281 tggatgaccc aaattattga acaatatagt tacttcggta tcttactgat gatggcactg
    17341 gaaaacatct ttccacccat accttctgag gtgatcttga cgttcggtgg atttatgaca
    17401 acgcaatcat ccttaacggt ttttggcgta atcatgtcag ctacaatcgg ttcggtaatc
    17461 ggggcaatca ttctctatgg aatcggttat tacctagacg tcaaaaaaat agagaagatc
    17521 gtagcgagat gggggcatat tctgcggatt aaaccggcag atatccataa agcgaatgaa
    17581 tggttcgacc gatacggata ctggacgatc tttttttgcc gtatggtccc attaatcaga
    17641 agcctgattt ccattccagc aggtatgacc aaaatgaatt tcccgctgtt tttgttgttt
    17701 acaacacttg gtacgtttat ttggaacatc gtcctcgtga tggctggtca cttattgggg
    17761 gaatcgtggg aagacattct gtactacttc gatctctatt ccaatgtcgt ttatgttgta
    17821 ctcgcatttg cgggggtagc gttcctcatt tttttcctcc gcaaccgaaa agcggatgcc
    17881 taaaaccttc aaaccatctt tctttcaagg gaaggtggtt tttccatttg gaacctaaag
    17941 gtaaacatat tataaaaagt cgtaattttc ttgcaaatac aagatgccaa aattgagcta
    18001 aggtgtaggg agggggagta ggtgttatgg gcaccgccct tatatttgag gctgggggag
    18061 atgcatcttg atcgatttgt tacttgtaaa cggggtagtc attaccatgg acaaagaccg
    18121 tcgggtatta caggacgggg cagtcgccat tgataaagga agaattttgg aggtaggcga
    18181 cactccgctt ctaaaagaca agtatcccgc gcaaaaggta gttgactgcg cgcatcattg
    18241 tatattgcca ggcttcgttg acgcacatgg tcatggcgga cattcgttat tcaaaacgat
    18301 tgcgacggac aatatcaacg actggatgcc aatcatgacc aacacttata aacactacgt
    18361 gacagacgat ttctggtact acgagggaaa gctgtcagcg ttggagcggc taaaggctgg
    18421 aattacgaca ggggttagcg tactaggctc gatgccacgc tcagatgatc cgatctttgc
    18481 tttgaatcac gccaaagcgt acaacgaggt tggcgttcgt gaaatcgtag cgacaggtcc
    18541 atgcaaccca ccgtggccgc atcccttcag ccgctgggtc gatggacaaa aagtaacgaa
    18601 ggaagtcacg tatgagcagg ttctcgctgg agcagaagca gtgatcgaag cactaaatca
    18661 cgcgaatcac gataaaacca gagcttatat aactccattt gtcatcgtga cctctgtcaa
    18721 tccgtcctat ccgacgccag ctgaccaatt gtttggccta acggatttcg accgttacca
    18781 agcccggaaa atcagggaga ttgcgaaaaa gtacgacacg cgcatccatt ccgatgcttt
    18841 tggaggcatg attcacctcg cgattcaaga caaagagtac gcgctgctcg gcccagacgt
    18901 acacttgcag cattgtcggg gaatttcttt tgacgaggcg aaaattttgg ccgaaacagg
    18961 aacgaatgtg agtgcttctc ctggttttgg tcaaattaat gcacgcaccc cgattacgga
    19021 attgctggag atgggagcga ctgttgcgat caccacagac ggcacctcgc cgatgactcc
    19081 ttttgacatg ttccaggcta tgcgcagaat gcaactgctg caacaagcgg cgctgcgtga
    19141 ctattattac ctcccgccgg gcaagctgct ggaaatggtc acgattgatg cggcacgctg
    19201 tgtcggctgg gatgacgagc tggggtcgct tgaggctggt aaaaaagcgg acgtcattac
    19261 tgtaaatatg catcaggccc acttgaatcc ggagaacatg catgtccatc gtgtcgtcta
    19321 tcaggcagtc ggcggagatg tgaaccatgt cattgtcgat ggggaactaa ttatggacga
    19381 gcgtcgtgtg ttgactgtgg atgaaaagca aatcctcaaa gaagcgaatg aagaggcgaa
    19441 tcgcacgatc gagcgggcag gtctggaaac ctacatgcag ccaagcaagt atttctgggg
    19501 gcatgcgcga gcttatttgg atgagagaag atttgatccg acgaagttgt aggggaaaaa
    19561 agtagtaaag taggtgaggt gttatgctgc acataaaact tggatagtgc attgaatagg
    19621 agaaggtgcc cgccgccttc ccttttttat tggattatag ttgacaaaaa cgataggatt
    19681 aatcattatt tatttcataa tccccatcga caaagttgga ataaagaggt gcagccatgt
    19741 atctgactat tgataatgtt acgaaacatt ttgtgaatga gcagaaacaa aaagtaaagg
    19801 tgctggatga tatcaatctt gaagtggaaa agggcagctt cgtttccatt gtcggcccct
    19861 ctggctgtgg aaaatcgacg ctgctctact tgatagcggg attggatcaa cctgatagcg
    19921 gtgacataca cgttgctggc aacaaagtga ataagcccgg gcccgacaga gttgtcgttt
    19981 ttcaagaagc aggattattt ccatggctga ccgttttgga aaatgttaca tacgggctca
    20041 agttgaagaa aatgtctaca gcgaaagcca acgaaaaggc cctggaagtg ctcaaaatgg
    20101 tccatttaag caagtatgtt cattcctatc cccatcagct ctcaggcggg atgaagcaac
    20161 gtgtagccat agctcgggca cttgtcatgg aacccgacat tttgctgatg gatgaacctt
    20221 tctcggcgct ggatgaacag acgcggatgg ttcttcataa ggagttgctt gagatttggc
    20281 ggacgaccaa ggtcacgatt tttttcgtca cccataatat ccgggaggct gttcagcttg
    20341 cggagaggat cgtcgttttt gccacacgcc ccggaaaaat caaggaaatc attgcggttc
    20401 ctgctatgcg cgatggagtc atgccggaca gtgtgacagt aaacacagag cagaaggtgc
    20461 tgtcgatcct gcaagaagag atagaaaagg tgctgaagga ggaaatgggc aatgactaca
    20521 gctttaagac gggtcatctt catcgctatg atagcgggga tctgggaagt cacatctaaa
    20581 ttatctggac tgccatcgtt catgtttccc agcttgaccc aggttttcga aacactcgta
    20641 aatggcttga taagcgggca aattaccgcc gcgattggaa aaagcatggg acgaatcctg
    20701 ttgggctttc tcattgccat tatcgtcggt ctgatattgg gctacttcat ttggagatac
    20761 aaactcgtag aagatacgct cggctttgtc gtgacggcgc ttcaatccat cccgagcatt
    20821 gtctggttcc cgcttgccat tatctggttt ggcttaaatg acttttccat tctgttcatc
    20881 gtgacgatcg gggcgacctg gacgatgacg gtaaacgcga ccagcggttt caaaaatgtc
    20941 ccgccgctat atcagcgggt agccaggact tacggttcga ctggctttca ctttgtacga
    21001 acggtgattc ttcctgcatc cgtaccgcaa atcatctctg ggcttcgaat tgcttgggca
    21061 ttttcctggc gggcactcat ggctggtgag ctgctcgggg gtggcggtgg tctcggtcaa
    21121 ttgctcgaga tgggacgttc acttggacaa atggatttgg tgatatctgt catgatcatt
    21181 attgcgatca tcggtaccat cgtcgacaat gttgtctttt cacggatcga acgcaacgtt
    21241 cagatgaagt ggggagttca ttcataaaac gacaaatagc gaaggagtgt gtcacatgct
    21301 taaaaaattg ttcagtaccg ttgtcgcatc cctattattg atgggggttg cgagcgggtg
    21361 ctctacaggc ggagcagggt cctctgagaa ggaaataaag atcggatatt tcccaaatct
    21421 gacccacagc gcaacgatca ttgcactgga aaaaggctat tttaaggaag ccttcggtgc
    21481 tgatatgaag attcaaacaa aaacggtcac caatggcgga ttgtttatgg aagcgatggc
    21541 taccaaagca attgatgtag ggacagtcgg accaggacca ctgttgaact tttatgtcaa
    21601 gcatcccgga tatcgcttga tttcaggtgc ggtcaatggc ggagcagtcc tcgtcatgaa
    21661 tggttcaacc actattacgg aactaaaaga tgtaaaaggc aaaaaaatcg cgattccggt
    21721 aatcggcagc acacaggatg tcatgctaag aaaagcactg aatgaggttg gactgaaacc
    21781 gacgacgaat ggtggcgatg tggaactgta tgcggcggct ccggcagata cggcagcatt
    21841 atttgtacaa aaatccgttg acggtgctgc aacgcaggag ccgtggggat atgtactgga
    21901 aaatcaggcg ggaggaaaat tgcttttgga ctgggatcaa ttcgcttggg ggaaagaatc
    21961 aaccaatacc gtggtagcag ccagcgatga atttttgaaa cgagaaggat tggcaaccgc
    22021 ctacctgcaa gcacataaaa aggcagtcaa attcattcag gaaaatcccg aagaaagcca
    22081 agacctgatc atcaagcatt tgaaaagtct gactggcaaa gagctgagca aaaaagaagt
    22141 gcaggcagct ttttcccgtt tggaagtaac aacggcggtc aatgaaaaag ttattcagga
    22201 aatggcagat attagccagg aagccggcta tatttccagt aataaaatcg atggcttgat
    22261 cgatttgaaa tacttagaag cagcgaaata atatcgggcg agaaggaggg agattcagca
    22321 ggaggaatga ggggttgaaa ggaattagat gggaaaggag gtcatgccgc gatgagacca
    22381 catgaacaag gaagtaatca acctaacgac acctcactgg aaacacagat tcatactgat
    22441 ttccaaaaag aaatgacgta cggcgattat ttacagctcg atcaaatctt gtcgagtcaa
    22501 cagcgtcaat ctacccatca tgatgaaatg ctgtttatta tcattcatca ggtgagtgag
    22561 ctatggatga aacaaatttt gcacgaactg tcagcagcaa atgagtgcat ctgcaatcac
    22621 gatctggagc cagctttcaa aatgttcgcg cgagtctcgc gtatccagca gcaattgatt
    22681 aaatcgtggg atgtcctttc tacattgacg ccagccgatt acctccagtt ccgtgacaag
    22741 ctgggtcatt cgtccggatt tcaatcctat caaaaccgac tcatcgaatt tacgctagga
    22801 tataagaacc agcatgtcct cgcggtgtat gcgcatcaac cagagcttca cgcccaaatg
    22861 agtgcggctt tgcagcagcc cagcatttac gatgcggcga tcagggagat ggctgctcgc
    22921 ggcttgccga ttgatccgga atgtttgaat cgcgactggt cacagccgta tcaaccaaac
    22981 cagagtgtag agcaagcttg gctgaccgtg tatcgaaatg taaaccagta ttgggatttg
    23041 tacgagttgg cagaaaagct ggtggacatc gccagtcagc aacaacaatg gcgttttaac
    23101 catatgacga cagtcgaacg catcataggc caaaagccgg gaacaggagg gtcctcaggc
    23161 gtcatgtact tacgcagggc gctcgatcac cgttttttcc cggagctttg gagcttgcgg
    23221 acgttgttgt aaaaaatctt caaataaagc gagaaaagtg tcttggggac ccagggcgct
    23281 tttttcgctt gtagtactcc tttcacattt tttcacatat cttacctaat attgggtaca
    23341 aatacccttc ttttcctaac aaacatatgt tttcattttt aatagtgtac ctattttttc
    23401 aagaacaacc agaaatggag tgggcatttg gatggaaaca atgaagccac ctatggaagt
    23461 gttatatgcg aaacagctga atgcgctgcg atcccatgat acagggacaa aacctcccaa
    23521 ttggctgatg tcgcccagag ctgtgcgcga ctttatttta ggaacggatg a
//
