LOCUS       NZ_KQ040797             5905 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Paenibacillus wulumuqiensis strain Y24 Scaffold22, whole genome
            shotgun sequence.
ACCESSION   NZ_KQ040797
VERSION     NZ_KQ040797.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus wulumuqiensis
  ORGANISM  Paenibacillus wulumuqiensis
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Paenibacillaceae; Paenibacillus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Wu,Q., Zhu,L., Jiang,L., Xu,X., Xu,Q., Zhang,Z. and Huang,H.
  TITLE     Genome Sequence of Paenibacillus wulumuqiensis sp. nov., a
            Bioflocculant-Producing Species
  JOURNAL   Genome Announc 3 (4), e00795-15 (2015)
   PUBMED   26184946
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Wu,Q., Jiang,L. and Xu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2015) College of Biotechnology and Pharmaceutical
            Engineering, Nanjing Tech University, No. 30 South Puzhu Road,
            Nanjing, Jiangsu 211816, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000971965.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 14:25:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,570
            CDSs (total)                      :: 4,478
            Genes (coding)                    :: 4,431
            CDSs (with protein)               :: 4,431
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 10 of 47
            Pseudo Genes (incomplete)         :: 37 of 47
            Pseudo Genes (internal stop)      :: 6 of 47
            Pseudo Genes (multiple problems)  :: 6 of 47
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:39:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5905
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KQ040797.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..2316
                     /locus_tag="WH45_RS08240"
     CDS             <1..2316
                     /NRPS_PKS="Domain: AMP-binding (163-561). E-value: 1.4e-94.
                     Score: 309.0. Matches aSDomain:
                     nrpspksdomains_WH45_RS08240_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (676-740). E-value: 4.3e-22. Score:
                     70.1. Matches aSDomain: nrpspksdomains_WH45_RS08240_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="WH45_RS08240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_046213929.1"
                     /sec_met_domain="AMP-binding (E-value: 2.8e-107, bitscore:
                     346.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.2e-16, bitscore:
                     47.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YVHQVKEKCLGAYAHQEVSFERVVEAVQPERQRSYHPLFQVMFIH
                     HHDLSKNDTDFIQGAEVAGVNGHPDQAKFDLTLSVYEHAQGMDIQLRYRTELLTARQVD
                     RLANHLIHLLYEISIDSNVQVGSLNLLPPAELRTWISEQSDYSLQETPITNSIYDQVEL
                     QMNHSPSAIALEYGGESLSYGALKTRVEQLAFHLTQIQAVRRQDRVGIYMDKSMDAVIA
                     ILAVVKLGGCIVPVDTTYPQQRQQYMAAHAAPCCMLTTQLLREQAEAAFEMPVIVAEAN
                     VYNSCPAGIQTQAASSDAAYIVYTSGSTGRPKAIINHHRGIVNYISFMKKELKIGTDDT
                     VLQLASLSVDGFFRDLIAPLALGARVVLMSYEQARDHDWVIRMIARRQITCLLSTVPSL
                     LKSLAIHAEHARLKLHSMRIMMCSGEVLSDHLALRIKPLFPQSDLINLYGPTETTLTTT
                     YRRMGEEPEAVYDVGKPIDHTAVYILDHNRLPVPDGLVGEVYIGGHGMTRGYFNDEQQT
                     AQHFVQLPWADPALVFYRTGDRGFIRENGHLALKGRLDRQVKVRGYRVELNEVESALES
                     HPAVHTAIAVMRQDSDQHAELEGFILKSADCSGYDLDEIVSDLKSVFPMHLIPSAMFVL
                     QSIPMTPNGKIDRKQLKQYILPPVAAAENDTGDMDTLDIRQQRLVGIWEEVLQYDGIRI
                     RDNFFTIGGHSLLAAQVISRIREQFEVDIPLTALFDYPSIFELDKHFDKIAQPRILQSA
                     IGRRTRNFNSDFTNSNKR"
     source          1..5905
                     /db_xref="taxon:1567107"
                     /isolation_source="cold spring"
                     /mol_type="genomic DNA"
                     /organism="Paenibacillus wulumuqiensis"
                     /strain="Y24"
                     /submitter_seqid="Scaffold22"
                     /type_material="type strain of Paenibacillus wulumuqiensis"
     region          1..5905
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..5905
                     /SMILES="NC([*])C(=O)NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /kind="neighbouring"
                     /product="NRPS"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..5905
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2321:>5646](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2322..>5646
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     protocluster    1..5905
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2316](+)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      <1..2316
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="2"
     CDS_motif       19..138
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08240_0001"
                     /evalue="1.60E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="WH45_RS08240"
                     /protein_end="45"
                     /protein_start="5"
                     /score="68.7"
                     /tool="antismash"
                     /translation="KEKCLGAYAHQEVSFERVVEAVQPERQRSYHPLFQVMFIH"
     aSDomain        493..1686
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_WH45_RS08240_AMP-binding.1"
                     /evalue="1.40E-94"
                     /label="WH45_RS08240_AMP-binding.1"
                     /locus_tag="WH45_RS08240"
                     /protein_end="561"
                     /protein_start="163"
                     /score="309.0"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="QMNHSPSAIALEYGGESLSYGALKTRVEQLAFHLTQIQAVRRQDR
                     VGIYMDKSMDAVIAILAVVKLGGCIVPVDTTYPQQRQQYMAAHAAPCCMLTTQLLREQA
                     EAAFEMPVIVAEANVYNSCPAGIQTQAASSDAAYIVYTSGSTGRPKAIINHHRGIVNYI
                     SFMKKELKIGTDDTVLQLASLSVDGFFRDLIAPLALGARVVLMSYEQARDHDWVIRMIA
                     RRQITCLLSTVPSLLKSLAIHAEHARLKLHSMRIMMCSGEVLSDHLALRIKPLFPQSDL
                     INLYGPTETTLTTTYRRMGEEPEAVYDVGKPIDHTAVYILDHNRLPVPDGLVGEVYIGG
                     HGMTRGYFNDEQQTAQHFVQLPWADPALVFYRTGDRGFIRENGHLALKGRLDRQVKVR"
     aSModule        493..2223
                     /complete
                     /domains="nrpspksdomains_WH45_RS08240_AMP-binding.1"
                     /domains="nrpspksdomains_WH45_RS08240_PCP.1"
                     /locus_tags="WH45_RS08240"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       901..960
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08240_0002"
                     /evalue="7.10E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="WH45_RS08240"
                     /protein_end="319"
                     /protein_start="299"
                     /score="24.7"
                     /tool="antismash"
                     /translation="AYIVYTSGSTGRPKAIINHH"
     CDS_motif       1477..1566
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08240_0003"
                     /evalue="6.90E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="WH45_RS08240"
                     /protein_end="521"
                     /protein_start="491"
                     /score="37.6"
                     /tool="antismash"
                     /translation="PDGLVGEVYIGGHGMTRGYFNDEQQTAQHF"
     CDS_motif       1657..1716
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08240_0004"
                     /evalue="1.10E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="WH45_RS08240"
                     /protein_end="571"
                     /protein_start="551"
                     /score="30.4"
                     /tool="antismash"
                     /translation="GRLDRQVKVRGYRVELNEVE"
     aSDomain        2032..2223
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_WH45_RS08240_PCP.1"
                     /evalue="4.30E-22"
                     /label="WH45_RS08240_PCP.1"
                     /locus_tag="WH45_RS08240"
                     /protein_end="740"
                     /protein_start="676"
                     /score="70.1"
                     /tool="antismash"
                     /translation="QRLVGIWEEVLQYDGIRIRDNFFTIGGHSLLAAQVISRIREQFEV
                     DIPLTALFDYPSIFELDKH"
     gene            2322..>5646
                     /locus_tag="WH45_RS08245"
     CDS             2322..>5646
                     /NRPS_PKS="Domain: Condensation_LCL (10-309). E-value:
                     1.1e-81. Score: 266.0. Matches aSDomain:
                     nrpspksdomains_WH45_RS08245_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (472-869). E-value: 8e-105.
                     Score: 342.7. Matches aSDomain:
                     nrpspksdomains_WH45_RS08245_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (977-1046). E-value: 4.4e-26. Score:
                     82.9. Matches aSDomain: nrpspksdomains_WH45_RS08245_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="WH45_RS08245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_200897670.1"
                     /sec_met_domain="Condensation (E-value: 3.2e-70, bitscore:
                     224.2, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 5.2e-110, bitscore:
                     355.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.9e-18, bitscore:
                     53.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDYSNVPVFEAPMSCGQQRLWFLQNLDPDTSAYHIPFALTITGPL
                     SVSRFSDALGIIVAGHEPFRTRFAYTDSRYQQIIYAEAALNFDYMEYTADPERNLHDAM
                     QFIMQDKQQAFRLEQEFPYRFYLFRLAEEQYLFYANIHHIMFDRASFPVFMAELLNYYH
                     SPEAESVLDEQQDIQYADYCFWQQEQLEQGHLASQQNYWLNELQQSPVLQLPVQASGSK
                     SGEKQRGKAECRLFQLPAEMYESLQKWCRSEGITLNMGLLSAYNVLLSMYTGQSDLIVG
                     TPSAGRNRREVENTIGFFVNTLLIRSRLNMQQNFRTYVKQIKQKCLQAYENQDLPFDKL
                     VEMMNPERDAGTASPFQTIFSLQNRLDTSWNMGEIQLEMMELHNEDAKCDLALLMSETE
                     EGLFGEWQYDEALWQPADLDRMTIHFESILQFVLQHPEQALQEMDILTPEEHRIIHEMN
                     RTASDYPEQSVIYLFEQQAMQTPNATAIEEDGVTVSYQELNRSASLLARKLENESVGAG
                     QTVGICIGRSTAFVVSVLAVLKCGAVYVPLDPQLPEERMDYILRDSGVALVISDQASTE
                     KLDHSPVRIMVYKELGDDGQPDRWSNADNTSDQLAYLIYTSGSTGQPKGVAVRHKGIVR
                     LVQNTNYIDIQPEDRMAQTMNVSFDISTFEIWGALLNGAALVIMPQTVLISPSRLKQWL
                     TDQQISVMAISAALFHSTARTQPDAFVTLQSLLIGGEALSSRWVGEVAAAGAPKRMLNS
                     YGPSENTAITLNHEIDIHELDTDQSIPIGTPVSNTYVYVLNKYGQQLPVGVTGELYIGG
                     EGLAAGYWNRPELTAERFVHLPQLSDGPLYRTGDLVQRRADGQIDFIGRTDDQIKLRGH
                     RIELGEIEEALRSCPGICEAVAALTTVSGQEEPVLAVYYATEQELEAGQLRDQLQQRLP
                     AYMVPAAWREVASFALTVNGKIDRRQLPPIQEQDLRQQAYQQPDRPGEAEIAAIWADLL
                     NRQQIGAQDHFFAIGGHSLLAAQVVSRMEEQLQRQVTLRALFDYPVLRELAAYVQQQPV
                     SGPASVIPAYVSEDGRYPLSPAQQRLWLVQQLEPDSQAYHIPFAFHLYGILDADRLEQA
                     I"
     aSDomain        2352..3248
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_WH45_RS08245_Condensation_LCL.1"
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.10E-81"
                     /label="WH45_RS08245_Condensation_LCL.1"
                     /locus_tag="WH45_RS08245"
                     /protein_end="309"
                     /protein_start="10"
                     /score="266.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMFDR"
                     /tool="antismash"
                     /translation="APMSCGQQRLWFLQNLDPDTSAYHIPFALTITGPLSVSRFSDALG
                     IIVAGHEPFRTRFAYTDSRYQQIIYAEAALNFDYMEYTADPERNLHDAMQFIMQDKQQA
                     FRLEQEFPYRFYLFRLAEEQYLFYANIHHIMFDRASFPVFMAELLNYYHSPEAESVLDE
                     QQDIQYADYCFWQQEQLEQGHLASQQNYWLNELQQSPVLQLPVQASGSKSGEKQRGKAE
                     CRLFQLPAEMYESLQKWCRSEGITLNMGLLSAYNVLLSMYTGQSDLIVGTPSAGRNRRE
                     VENTIGFFVNTLLIRSRL"
     aSModule        2352..5459
                     /complete
                     /domains="nrpspksdomains_WH45_RS08245_Condensation_LCL.1"
                     /domains="nrpspksdomains_WH45_RS08245_AMP-binding.1"
                     /domains="nrpspksdomains_WH45_RS08245_PCP.1"
                     /locus_tags="WH45_RS08245"
                     /monomer_pairings="X -> X"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       2361..2393
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0001"
                     /evalue="1.60E-02"
                     /label="C1_LCL_004-017"
                     /locus_tag="WH45_RS08245"
                     /protein_end="24"
                     /protein_start="13"
                     /score="8.2"
                     /tool="antismash"
                     /translation="SCGQQRLWFLQ"
     CDS_motif       2412..2528
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0002"
                     /evalue="4.20E-13"
                     /label="C2_LCL_024-062"
                     /locus_tag="WH45_RS08245"
                     /protein_end="69"
                     /protein_start="30"
                     /score="41.7"
                     /tool="antismash"
                     /translation="SAYHIPFALTITGPLSVSRFSDALGIIVAGHEPFRTRFA"
     CDS_motif       2742..2807
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0003"
                     /evalue="2.60E-06"
                     /label="C3_DCL_135-156"
                     /locus_tag="WH45_RS08245"
                     /protein_end="162"
                     /protein_start="140"
                     /score="20.0"
                     /tool="antismash"
                     /translation="IHHIMFDRASFPVFMAELLNYY"
     CDS_motif       2853..2891
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0004"
                     /evalue="7.80E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="WH45_RS08245"
                     /protein_end="190"
                     /protein_start="177"
                     /score="12.2"
                     /tool="antismash"
                     /translation="QYADYCFWQQEQL"
     CDS_motif       2907..2933
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0005"
                     /evalue="4.10E+01"
                     /label="C1_starter_004-017"
                     /locus_tag="WH45_RS08245"
                     /protein_end="204"
                     /protein_start="195"
                     /score="-1.5"
                     /tool="antismash"
                     /translation="ASQQNYWLN"
     CDS_motif       3150..3239
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0006"
                     /evalue="1.40E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="WH45_RS08245"
                     /protein_end="306"
                     /protein_start="276"
                     /score="46.4"
                     /tool="antismash"
                     /translation="DLIVGTPSAGRNRREVENTIGFFVNTLLIR"
     CDS_motif       3288..3407
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0007"
                     /evalue="4.60E-16"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="WH45_RS08245"
                     /protein_end="362"
                     /protein_start="322"
                     /score="51.1"
                     /tool="antismash"
                     /translation="KQKCLQAYENQDLPFDKLVEMMNPERDAGTASPFQTIFSL"
     aSDomain        3738..4928
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_WH45_RS08245_AMP-binding.1"
                     /evalue="8.00E-105"
                     /label="WH45_RS08245_AMP-binding.1"
                     /locus_tag="WH45_RS08245"
                     /protein_end="869"
                     /protein_start="472"
                     /score="342.7"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FEQQAMQTPNATAIEEDGVTVSYQELNRSASLLARKLENESVGAG
                     QTVGICIGRSTAFVVSVLAVLKCGAVYVPLDPQLPEERMDYILRDSGVALVISDQASTE
                     KLDHSPVRIMVYKELGDDGQPDRWSNADNTSDQLAYLIYTSGSTGQPKGVAVRHKGIVR
                     LVQNTNYIDIQPEDRMAQTMNVSFDISTFEIWGALLNGAALVIMPQTVLISPSRLKQWL
                     TDQQISVMAISAALFHSTARTQPDAFVTLQSLLIGGEALSSRWVGEVAAAGAPKRMLNS
                     YGPSENTAITLNHEIDIHELDTDQSIPIGTPVSNTYVYVLNKYGQQLPVGVTGELYIGG
                     EGLAAGYWNRPELTAERFVHLPQLSDGPLYRTGDLVQRRADGQIDFIGRTDDQIKLR"
     CDS_motif       3924..3965
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0008"
                     /evalue="1.90E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="WH45_RS08245"
                     /protein_end="548"
                     /protein_start="534"
                     /score="17.8"
                     /tool="antismash"
                     /translation="LAVLKCGAVYVPLD"
     CDS_motif       4152..4211
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0009"
                     /evalue="4.90E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="WH45_RS08245"
                     /protein_end="630"
                     /protein_start="610"
                     /score="31.3"
                     /tool="antismash"
                     /translation="AYLIYTSGSTGQPKGVAVRH"
     CDS_motif       4722..4811
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0010"
                     /evalue="1.60E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="WH45_RS08245"
                     /protein_end="830"
                     /protein_start="800"
                     /score="58.7"
                     /tool="antismash"
                     /translation="PVGVTGELYIGGEGLAAGYWNRPELTAERF"
     CDS_motif       4893..4958
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0011"
                     /evalue="6.60E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="WH45_RS08245"
                     /protein_end="879"
                     /protein_start="857"
                     /score="37.3"
                     /tool="antismash"
                     /translation="FIGRTDDQIKLRGHRIELGEIE"
     CDS_motif       5109..5183
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0012"
                     /evalue="1.80E+01"
                     /label="C2_LCL_024-062"
                     /locus_tag="WH45_RS08245"
                     /protein_end="954"
                     /protein_start="929"
                     /score="-2.1"
                     /tool="antismash"
                     /translation="PAYMVPAAWREVASFALTVNGKIDR"
     aSDomain        5253..5459
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_WH45_RS08245_PCP.1"
                     /evalue="4.40E-26"
                     /label="WH45_RS08245_PCP.1"
                     /locus_tag="WH45_RS08245"
                     /protein_end="1046"
                     /protein_start="977"
                     /score="82.9"
                     /tool="antismash"
                     /translation="EAEIAAIWADLLNRQQIGAQDHFFAIGGHSLLAAQVVSRMEEQLQ
                     RQVTLRALFDYPVLRELAAYVQQQ"
     CDS_motif       5514..5555
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0013"
                     /evalue="1.20E-02"
                     /label="C1_starter_004-017"
                     /locus_tag="WH45_RS08245"
                     /protein_end="1078"
                     /protein_start="1064"
                     /score="8.8"
                     /tool="antismash"
                     /translation="YPLSPAQQRLWLVQ"
     CDS_motif       5577..5645
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_WH45_RS08245_0014"
                     /evalue="1.00E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="WH45_RS08245"
                     /protein_end="1108"
                     /protein_start="1085"
                     /score="21.1"
                     /tool="antismash"
                     /translation="AYHIPFAFHLYGILDADRLEQAI"
     assembly_gap    5647..5656
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5657..>5905
                     /locus_tag="WH45_RS23740"
     CDS             5659..>5905
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="WH45_RS23740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_235338323.1"
                     /sec_met_domain="Condensation (E-value: 2.7e-13, bitscore:
                     37.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDSLRTQFGEESGEAYQQVTGNLPVLERSSLHVPAGEQKQHLQNL
                     LQREAWEPLSLTAAPPLRLRLIELEAGHHVLSWVIHH"
ORIGIN
        1 tatgtccatc aggtcaagga gaagtgcctg ggagcttacg cccaccagga agtgtccttt
       61 gagcgggttg tcgaggctgt ccagccggag cggcagcgga gctatcatcc tttgtttcag
      121 gtaatgttta ttcaccatca tgacttgagc aagaatgata ccgactttat acaaggtgca
      181 gaagtagccg gtgtgaatgg tcatcctgat caggccaaat ttgatttgac attgtcagtg
      241 tatgaacacg cccagggtat ggatattcag cttcgctatc ggacagagct gttaaccgca
      301 cgccaggtag atcgtctggc caatcattta attcacttac tttatgaaat tagtatagac
      361 agtaatgtac aagtaggcag tctgaatctg cttcctcctg cagagctgcg gacatggatc
      421 agcgaacaat ccgactactc gctgcaagaa acaccaatca ccaacagtat ctatgatcag
      481 gtggagctgc aaatgaatca ttctccttct gcgattgcac tggaatacgg aggagagagt
      541 cttagttacg gagcattgaa aacacgagtc gagcaattgg cttttcatct gacgcaaatc
      601 caggctgttc gtcgtcagga cagagtaggg atctatatgg ataagtcgat ggatgcggtc
      661 attgcgatac tcgccgtagt aaagctcggt ggatgcattg tgcctgtgga cacgacgtat
      721 ccacagcagc ggcagcagta tatggcagct catgcagcac catgctgtat gctgactacc
      781 caattgctgc gcgaacaggc agaagcggca tttgagatgc cggtgattgt agccgaagcc
      841 aatgtgtaca acagctgccc cgccggaata cagacgcagg ctgcctccag tgatgccgcg
      901 tatatcgtat acacttccgg cagtacgggc cgtccgaaag ctatcattaa tcatcaccgc
      961 gggattgtga actatatttc ttttatgaaa aaagagctga agatcggtac ggatgatacg
     1021 gtactgcagc tggcttcctt gtcagtggac ggatttttcc gggacttgat tgctccgctt
     1081 gcgcttggcg ccagagtggt cttgatgtct tatgagcagg cacgggatca tgactgggtc
     1141 atccggatga ttgcccggcg gcaaatcacc tgcttgctca gcacagtacc gtcgctgctc
     1201 aaatcacttg ccattcatgc cgaacatgcc cggttaaagc tgcattcgat gcgaatcatg
     1261 atgtgcagtg gtgaagtgct gtccgatcat ctggctctgc gtatcaagcc gcttttccct
     1321 caaagcgatc tgatcaatct gtacggtccg acagagacca cattaacaac aacctaccgg
     1381 cgtatgggtg aagaacccga ggctgtatac gatgtaggca agccgattga tcatacggca
     1441 gtatatattc tggatcataa tcgcttgccg gtaccggatg gtctggtcgg agaagtatac
     1501 attggcggtc atggcatgac cagaggttat ttcaacgatg aacagcaaac cgcacagcat
     1561 tttgtacagc tgccctgggc agatccagcc ctggtatttt atcgtacggg cgatcgtggg
     1621 tttattcgtg aaaatggaca tctggcactg aaaggaagat tggaccggca ggtaaaagtt
     1681 cgaggttatc gtgtggaact gaatgaagtg gaatccgctc ttgaatctca tcctgccgtt
     1741 catacagcta tagcggtaat gaggcaggat agcgaccagc atgccgagct cgaaggattt
     1801 attttaaaat cagccgactg ttccggatat gacctggatg agatcgtaag cgatttgaaa
     1861 tccgttttcc ccatgcattt gattccgtct gccatgtttg ttttgcagtc catcccgatg
     1921 acgcccaatg gaaaaattga ccgcaagcag ctgaagcagt atatcctgcc tccggttgcg
     1981 gcagcggaga atgatactgg tgacatggat acacttgata tccgtcagca gcgactggtc
     2041 ggtatctggg aagaagtgct gcagtatgac ggcattcgta tacgggataa tttctttacg
     2101 attggaggtc attccttgct tgctgcacag gtgatctcgc gcattcgtga gcagtttgaa
     2161 gtggatattc cgctgaccgc tttgtttgat tatccttcta tattcgaact ggataagcat
     2221 tttgacaaga tagcgcagcc gcgaatactg cagtctgcta taggcagaag aacacgcaat
     2281 ttcaattctg attttaccaa ttcgaataag aggtgaacca aatggattat tccaatgtgc
     2341 ccgtgtttga agctcccatg tcctgtgggc agcagcgtct gtggtttttg caaaatttgg
     2401 atcccgatac aagcgcatat catattccat ttgcccttac cattaccggt ccgctgtctg
     2461 tctcccgttt cagtgatgct ttggggatca tcgttgccgg acatgagccg ttccgtacgc
     2521 gctttgccta taccgattcc cggtatcagc aaatcattta tgccgaagct gcattgaatt
     2581 ttgattatat ggaatacacc gctgatcccg agcggaattt gcatgatgcc atgcagttta
     2641 ttatgcagga taagcagcag gctttcaggc tggaacagga atttccctat cggttttatc
     2701 tatttcgtct ggcagaagag cagtatctgt tttatgccaa tattcaccat attatgtttg
     2761 accgcgcttc atttccggta ttcatggcgg agctgctgaa ctattaccat tcacctgaag
     2821 cggaatccgt tttggacgag cagcaggata ttcagtatgc ggattactgt ttctggcagc
     2881 aggagcagct ggaacaggga catctggcat cccagcagaa ctactggctg aacgagcttc
     2941 agcagtctcc tgttttacag ctgcccgtac aagcttcggg aagcaaatcc ggagaaaaac
     3001 aacgcggaaa ggcagaatgc cgattattcc agcttccggc cgaaatgtac gaatcgctcc
     3061 aaaaatggtg cagatcagaa ggaattactt tgaatatggg tctgctatcc gcgtataacg
     3121 tgctgttatc catgtatacc ggtcagtctg atctgattgt aggcacaccg tcagcagggc
     3181 ggaatcggcg tgaggttgaa aatacgattg gatttttcgt aaatacactg ctgattcgtt
     3241 cccggttgaa tatgcagcaa aatttccgaa cgtatgtaaa gcagatcaaa caaaaatgtc
     3301 tgcaggcgta tgagaatcag gatcttccat ttgataaact ggtcgaaatg atgaatcccg
     3361 agcgtgatgc aggaacagcc tctccttttc agaccatatt ttccctgcaa aacagactgg
     3421 ataccagctg gaatatgggt gaaattcagc tggaaatgat ggagctgcat aatgaggatg
     3481 ccaaatgtga tcttgctctg ttgatgagtg agacggagga aggactgttc ggagaatggc
     3541 agtatgatga agcgctatgg cagccggcag atctggaccg gatgaccata cattttgaat
     3601 ccattctgca atttgttcta caacatccgg aacaggcact acaggaaatg gatatactga
     3661 caccggaaga gcatcgaatc attcatgaga tgaatcgaac agcttccgat tatccggaac
     3721 aatcggttat ttatttgttc gaacagcagg cgatgcagac accgaatgcg actgctatcg
     3781 aagaggatgg ggtcacggta agttaccagg aactgaatcg gtcggccagc ctgcttgccc
     3841 gcaagcttga aaatgaatct gtcggtgccg gacagacagt aggcatatgc atcggtcgtt
     3901 ccactgcatt tgtggtatcg gtactggcag tgctgaaatg cggagctgtc tatgttccgc
     3961 ttgacccgca gcttccggag gaacgcatgg attatatttt gcgggacagt ggagttgctc
     4021 tggtgatcag tgatcaggca tctaccgaaa agctggatca ttctcctgtt cggatcatgg
     4081 tctacaagga gctgggtgat gatgggcagc ctgacagatg gagtaatgcc gacaatacat
     4141 ccgatcaact tgcttacctc atttatactt caggatctac cgggcagccc aaaggggtag
     4201 cggtcagaca taaaggaatt gtgcgtctcg tccaaaatac caattacatt gatatacagc
     4261 ctgaagaccg tatggcccag acgatgaatg tttcctttga tatttccacc tttgaaatat
     4321 ggggtgcgtt gttaaatgga gcagctctgg ttattatgcc gcagaccgtg ctaatatctc
     4381 cgtcccggct caaacagtgg ctgaccgatc agcaaatcag tgtaatggcc atatcggccg
     4441 cattattcca tagtacagcg cggacccagc cggatgcgtt tgttacactt caatccctgc
     4501 taatcggcgg ggaagcactg agcagcagat gggttggcga agtggcagca gccggagcgc
     4561 ccaaacgtat gctgaattca tacggtccaa gtgagaacac ggcgattacc ctgaaccatg
     4621 aaattgacat ccatgagctt gatacggatc aatctattcc gattggtacg cctgtctcga
     4681 atacatatgt atacgtactg aataaatatg gtcagcagct gccggttgga gttaccggag
     4741 agctatatat cggcggtgaa ggactggcag caggctactg gaaccgaccg gagctgacgg
     4801 ctgagcgatt tgttcatctg ccgcagctat cggatgggcc gctgtaccgt accggtgatc
     4861 tggtccagcg gcgagcggat ggccagattg actttatcgg ccgaaccgat gatcagatca
     4921 agctgcgtgg tcaccgaatt gagctgggag agattgaaga agcgctgcgc agctgcccgg
     4981 gaatctgcga ggcagtggct gcattgacta ccgtatccgg tcaggaagag ccggtgctgg
     5041 ctgtgtacta tgcgaccgaa caagagctgg aggccggaca gctgcgtgac cagctgcagc
     5101 agcggctgcc tgcttatatg gtaccggctg cctggcggga agtggcatca tttgccctga
     5161 ccgtcaatgg caagattgac cgcagacagc tgccaccgat tcaggaacag gatctgcgtc
     5221 agcaggcgta ccagcagccg gatcgaccgg gagaagcaga gatagccgcc atctgggcgg
     5281 atctgctgaa ccggcagcag attggcgccc aggatcattt cttcgctatc ggagggcatt
     5341 ccctgctggc ggcgcaggtc gtcagccgca tggaagaaca gctgcagcgg caggtgaccc
     5401 tgcgagccct gtttgattat ccggtgctgc gggagctggc agcgtatgtg cagcagcagc
     5461 cggtaagcgg cccggccagc gtgattccgg cctatgtctc ggaagatggt cgctacccgt
     5521 tatcaccggc gcagcagcgg ttgtggctgg tccagcagct ggaaccggac agccaggcgt
     5581 atcatattcc ttttgccttt catctgtatg gcattttgga tgccgatcga ctggagcagg
     5641 ctattcnnnn nnnnnngcat gacagtttgc ggacgcagtt tggagaagag agcggggagg
     5701 cgtatcagca ggtaaccggc aatttgccag tcttggagcg ttcgagtctg catgtgccgg
     5761 caggagagca gaagcagcat ctgcagaacc tgctgcagcg ggaagcctgg gagccgctgt
     5821 cgctgacagc tgcaccgccg ctgcgcctgc gcctgattga gctggaagca ggtcaccatg
     5881 tgctgagctg ggtgatccac catat
//
