GCF_000966445.2 - 2 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP014241.1 (Bifidobacterium angulatum)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	terpene-precursor	1,354,724	1,375,803
Region 2	lanthipeptide-class-ii	1,953,269	1,976,379

NZ_CP014241.1 - Region 1 - terpene-precursor

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Location: 1,354,724 - 1,375,803 nt. (total: 21,080 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase

Bang102_RS05375: acyltransferase

smcogs: SMCOG1141: acyltransferase

Ligases: synthetase

Bang102_RS05385: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.38	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002309.2	0.36	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001911.3	0.35	terpene	raimonol	Streptomyces anulatus
BGC0001456.3	0.34	terpene	isorenieratene	Streptomyces argillaceus
BGC0001910.4	0.33	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000664.5	0.32	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001227.3	0.31	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.31	terpene	carotenoid	Streptomyces avermitilis
BGC0000648.3	0.31	terpene	carotenoid	Myxococcus xanthus
BGC0000644.3	0.31	terpene	carotenoid	Dietzia sp. CQ4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.38	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002309.2	0.36	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001911.3	0.35	terpene	raimonol	Streptomyces anulatus
BGC0001456.3	0.34	terpene	isorenieratene	Streptomyces argillaceus
BGC0001910.4	0.33	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000664.5	0.32	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001227.3	0.31	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.31	terpene	carotenoid	Streptomyces avermitilis
BGC0000648.3	0.31	terpene	carotenoid	Myxococcus xanthus
BGC0000644.3	0.31	terpene	carotenoid	Dietzia sp. CQ4

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

Bang102_RS05385: PT_FPPS_like

Domain 1 (49 - 351): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 23.51 out of 30.11

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.73 out of 18.46

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.46 out of 23.79

Confidence: medium

Additional weak hits (13):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.25 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 18.01 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 18.21 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.83 out of 26.06

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.41 out of 30.11

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 21.05 out of 30.97

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.98 out of 29.65

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.40 out of 26.12

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 21.06 out of 35.63

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 19.56 out of 33.14

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 23.53 out of 29.65

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.29 out of 27.04

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 20.95 out of 33.14

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CelR	Cellobiose uptake repressor
IolR	Regulator of the inositol catabolism
RpoN	Regulator of nitrogen assimilation and virulence

NZ_CP014241.1 - Region 2 - lanthipeptide-class-ii

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Location: 1,953,269 - 1,976,379 nt. (total: 23,111 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase hydratase

Bang102_RS07890: Nitrilase/cyanide hydratase and apolipoprotein

smcogs: SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein

Bang102_RS07910: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001555.4	0.51	ribosomal	colicin V	Escherichia coli chi7122
BGC0002698.2	0.49	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000504.5	0.49	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000623.3	0.48	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0000588.5	0.48	ribosomal	microcin L	Escherichia coli
BGC0000626.3	0.48	ribosomal	thuricin	Bacillus thuringiensis
BGC0000521.3	0.48	ribosomal	lacticin 481	Lactococcus lactis subsp. lactis
BGC0000528.5	0.48	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002699.2	0.48	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102
BGC0000500.5	0.47	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001555.4	0.51	ribosomal	colicin V	Escherichia coli chi7122
BGC0002698.2	0.49	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000504.5	0.49	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000623.3	0.48	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0000588.5	0.48	ribosomal	microcin L	Escherichia coli
BGC0000626.3	0.48	ribosomal	thuricin	Bacillus thuringiensis
BGC0000521.3	0.48	ribosomal	lacticin 481	Lactococcus lactis subsp. lactis
BGC0000528.5	0.48	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002699.2	0.48	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102
BGC0000500.5	0.47	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for Bang102_RS07910

Bang102_RS07925

Leader:

MSNTSIDVDSIVGDDFSDLSPEDMALLTG

Core:

RGNDVAPQSLSIAVSVDVTVSVAMSVASYFHVGGTCKKH

Core with dehydrations:

RGNDVAPQDhaLDhaIAVDhaVDVDhbVDhaVAMDhaVADhaYFHVGGDhbCysKKH

antiSMASH-DB 4.0 matches for Bang102_RS07925

100.0%

NZ_AP012322.1and 1 other:

NZ_CP014241.1 Lanthipeptides: Bang102_RS07925_lanthipeptide

Lanthipeptides: BBAG_RS05705_lanthipeptide

RGNDVAPQSLSIAVSVDVTVSVAMSVASYFHVGGTCKKH

23.1%

NZ_FTML01000041.1:

Lanthipeptides: BW937_RS20030_lanthipeptide

NDVAPQSLSIAVSVDVTVSVAMS

DV P+S V +TV +A+S

GDVQPESTPACVGAGITVGIAIS

14.3%

NZ_KB944819.1:

Lanthipeptides: WOK_RS14385_lanthipeptide

VDVTVSVAMSVASYF

+DVT+SVA S++

IDVTLSVAKRGKSWY

MIBiG 4.0 matches for Bang102_RS07925

10.3%

BGC0002322.3 (CEJ66604.1):

Lasso peptide: citrocin

SVASYFHVGGTCK

+ YF GG +

KIIEYFIGGGVGR

allorf_1955641_1955880

Leader:

MPFSHSRHGKRDVRAPELPGKRSYGRSVRFLSASRLPCHCPIIRSTLDFPSSLPRASAAVGA

Core:

ERAGACSFNPRKCSRMA

Core with dehydrations:

ERAGACysDhaFNPRKCysDhaRMA

antiSMASH-DB 4.0 matches for allorf_1955641_1955880

100.0%

NZ_CP014241.1:

Lanthipeptides: allorf_1955641_1955880_lanthipeptide

ERAGACSFNPRKCSRMA

27.3%

NZ_BMJA01000001.1:

Lanthipeptides: allorf_0181822_0181917_lanthipeptide

RAGACSFNPRKCS

RAG C P +C+

RAGRCQAEPHRCA

23.5%

NZ_CP059736.1:

Lanthipeptides: allorf_777630_777734_lanthipeptide

AGACSFNP

+G C +NP

SGVCKYNP

with 2 more sequence matches

22.2%

NZ_PIZC01000055.1:

Lanthipeptides: allorf_14138_14299_lanthipeptide

ERAGACSFNPRKC

E G C + PR C

EGFGWCLWGPRAC

19.4%

NC_009621.1:

Lanthipeptides: allorf_1031905_1032045_lanthipeptide

RAGACSFNPRK

RAGAC + RK

RAGACPKDERK

MIBiG 4.0 matches for allorf_1955641_1955880

21.1%

BGC0000503.5 (cinA):

Lanthipeptide: cinnamycin

ACSFNP

+CSF P

SCSFGP

allorf_1955751_1955870

Leader:

MSLPDNTQHARFPVFASTSIGG

Core:

CRRRTRGSMLLQSPEML

Core with dehydrations:

CysRRRDhbRGDhaMLLQDhaPEML

antiSMASH-DB 4.0 matches for allorf_1955751_1955870

100.0%

NZ_CP014241.1:

Lanthipeptides: allorf_1955751_1955870_lanthipeptide

CRRRTRGSMLLQSPEML

allorf_1958314_1958487

Leader:

MRYRQSQRCAFMEGG

Core:

RFGRYRAVGGSFGRNQADECRFGRNWARKRPNRHPTSGKRPN

Core with dehydrations:

RFGRYRAVGGDhaFGRNQADECysRFGRNWARKRPNRHPDhbDhaGKRPN

antiSMASH-DB 4.0 matches for allorf_1958314_1958487

100.0%

NZ_CP014241.1:

Lanthipeptides: allorf_1958314_1958487_lanthipeptide

RFGRYRAVGGSFGRNQADECRFGRNWARKRPNRHPTSGKRPN

23.8%

NZ_BNCA01000011.1and 1 other:

NZ_BNCA01000011.1 Lanthipeptides: allorf_142674_142883_lanthipeptide

Lanthipeptides: allorf_142674_142883_lanthipeptide

SFGRNQADECRFGRNWARK

+FGR RFGR W RK

AFGRWA--HLRFGRTWVRK

17.8%

NZ_KB900614.1:

Lanthipeptides: allorf_2952775_2952975_lanthipeptide

RPNRHPTSGKRP

RP RHPT+ RP

RPQRHPTTRFRP

with 1 more sequence matches

14.3%

NZ_FNPT01000006.1:

Lanthipeptides: allorf_202516_202659_lanthipeptide

RKRPNRHPT

RKRP+R P+

RKRPDRQPS

allorf_1968211_1968360

Leader:

MNEQATLSVIFPESADSGCSFVAQTRQMDSMTAS

Core:

IVLTVCCIGMTRLRS

Core with dehydrations:

IVLDhbVCysCysIGMDhbRLRDha

antiSMASH-DB 4.0 matches for allorf_1968211_1968360

100.0%

NZ_AP012322.1and 1 other:

NZ_CP014241.1 Lanthipeptides: allorf_1968211_1968360_lanthipeptide

Lanthipeptides: allorf_1398921_1399070_lanthipeptide

IVLTVCCIGMTRLRS

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 20.58 out of 23.05

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 17.62 out of 18.16

Confidence: strong

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.13 out of 26.85

Confidence: medium

Additional weak hits (8):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 25.70 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 21.91 out of 36.54

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 24.37 out of 39.62

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.65 out of 27.55

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.22 out of 30.98

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.35 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.04 out of 29.65

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.27 out of 20.01

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

Bang102_RS07925 - Class II

Cleavage pHMM score: -4.10

RODEO score: 0

Monoisotopic mass: 3872.0 Da

Molecular weight: 3874.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3892.4 Da N = 1; 3910.4 Da N = 2; 3928.4 Da N = 3; 3946.5 Da N = 4; 3964.5 Da N = 5; 3982.5 Da N = 6; 4000.5 Da N = 7

allorf_1955641_1955880 - Class II

Cleavage pHMM score: -3.90

RODEO score: 0

Monoisotopic mass: 1846.9 Da

Molecular weight: 1848.1 Da

Number of bridges: 2

allorf_1955751_1955870 - Class II

Cleavage pHMM score: -1.20

RODEO score: 0

Monoisotopic mass: 1979.0 Da

Molecular weight: 1980.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1998.4 Da N = 1; 2016.4 Da N = 2

allorf_1958314_1958487 - Class II

Cleavage pHMM score: -6.50

RODEO score: 0

Monoisotopic mass: 4860.5 Da

Molecular weight: 4863.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4881.4 Da N = 1; 4899.4 Da N = 2

allorf_1968211_1968360 - Class II

Cleavage pHMM score: -5.50

RODEO score: 0

Monoisotopic mass: 1609.9 Da

Molecular weight: 1611.0 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1629.1 Da N = 1

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsR	Pleiotropic regulatory for antibiotic production
RutR	Regulator of the pyrimidine and purine metabolism