LOCUS       NZ_CP009473            10852 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Burkholderia pseudomallei MSHR840 chromosome II, complete sequence.
ACCESSION   NZ_CP009473
VERSION     NZ_CP009473.1
KEYWORDS    RefSeq.
SOURCE      Burkholderia pseudomallei MSHR840
  ORGANISM  Burkholderia pseudomallei MSHR840
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Burkholderia; pseudomallei group.
REFERENCE   1  (bases 1 to 3108026)
  AUTHORS   Johnson,S.L., Bishop-Lilly,K.A., Ladner,J.T., Daligault,H.E.,
            Davenport,K.W., Jaissle,J., Frey,K.G., Koroleva,G.I., Bruce,D.C.,
            Coyne,S.R., Broomall,S.M., Li,P.E., Teshima,H., Gibbons,H.S.,
            Palacios,G.F., Rosenzweig,C.N., Redden,C.L., Xu,Y., Minogue,T.D. and
            Chain,P.S.
  TITLE     Complete genome sequences for 59 burkholderia isolates, both
            pathogenic and near neighbor
  JOURNAL   Genome Announc 3 (2), e00159-15 (2015)
   PUBMED   25931592
  REMARK    Erratum:[Genome Announc. 2016 Apr 21;4(2):e00313-16. doi:
            10.1128/genomeA.00313-16. PMID: 27103731] Publication Status:
            Online-Only
REFERENCE   2  (bases 1 to 3108026)
  AUTHORS   Johnson,S.L., Bishop-Lilly,K.A., Ladner,J.T., Daligault,H.,
            Jaissle,J., Frey,K.G., Koroleva,G.I., Bruce,D.C., Coyne,S.R.,
            Broomall,S.M., Li,P.-E., Teshima,H., Gibbons,H.S., Palacios,G.F.,
            Rosenzweig,C.N., McMurry,K., Redden,C.L., Xu,Y., Currie,B., Mayo,M.,
            Minogue,T.D. and Chain,P.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2014) Bioscience, Los Alamos National Laboratory,
            P.O. Box 1663, MS M888, Los Alamos, NM 87545, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 11-SEP-2014
            Assembly Method :: Newbler v. 2.6; Velvet v. 1.2.08; Allpaths v.
            44837; Phrap v. SPS - 4.24; HGAP v. 2.2.0
            Genome Coverage                   :: 661x
            Sequencing Technology             :: Illumina; PacBio; 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000959185.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 14:46:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,351
            CDSs (total)                      :: 6,275
            Genes (coding)                    :: 6,096
            CDSs (with protein)               :: 6,096
            Genes (RNA)                       :: 76
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 110 of 179
            Pseudo Genes (incomplete)         :: 100 of 179
            Pseudo Genes (internal stop)      :: 20 of 179
            Pseudo Genes (multiple problems)  :: 44 of 179
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:09:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1182806
            Orig. end                         :: 1193658
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP009473.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            The DNA was obtained from USAMRIID.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10852
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10852
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10852
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5852](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5852
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(85..321)
                     /locus_tag="BG19_RS04920"
                     /old_locus_tag="BG19_4447"
     CDS             complement(85..321)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196519.1"
                     /locus_tag="BG19_RS04920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BG19_4447"
                     /product="hypothetical protein"
                     /protein_id="WP_004196519.1"
                     /transl_table=11
                     /translation="MTRHHTHAGIPRTLKIVGYCGLAAIALALFVAMCAILKDSALDRE
                     AARQLQNGNVELHGGADVEPGSTNDAAQARTPG"
     gene            514..762
                     /locus_tag="BG19_RS41740"
     CDS             514..762
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194583.1"
                     /locus_tag="BG19_RS41740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_011852397.1"
                     /transl_table=11
                     /translation="MWRRADARASRDASGRWNLNARAGRRRCGVDRRENGGEANETALS
                     ARGMAAKACDAMGQRGAAALERRGGAVRSGANQSRLP"
     gene            complement(1086..1913)
                     /locus_tag="BG19_RS04925"
                     /old_locus_tag="BG19_4448"
     CDS             complement(1086..1913)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /GO_process="GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1200: vibriobactin
                     utilization protein ViuB"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532447.1"
                     /locus_tag="BG19_RS04925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BG19_4448"
                     /product="siderophore-interacting protein"
                     /protein_id="WP_004194445.1"
                     /transl_table=11
                     /translation="MTQPTTERAVVRVRHTLKFRLLQVKRVVDVTPHLKRVTLAGDELD
                     DFESASFDDHVKVFFPPPGEDAPARPEFGPNGIVFPQDRPRPVARDFTPRRYDRAAREL
                     DLDFVLHHPGPASQWAAQARVGQTLGIGGPRGSFVIPKDFDWHLLIGDDTALPAVARRL
                     EELPAGTRVAAVLEVADPSAQIAFETDANLYVVWRHRGDAASGERGASRLVDALRELPL
                     PAGEGYVWAAGEAAAMRGVREHLCRERGVAKSRIRASAYWKHGAIAVHESLED"
     gene            complement(1953..2669)
                     /locus_tag="BG19_RS04930"
                     /old_locus_tag="BG19_4449"
     CDS             complement(1953..2669)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548999.1"
                     /locus_tag="BG19_RS04930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BG19_4449"
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_038749976.1"
                     /transl_table=11
                     /translation="MRHAHRFSRRECGMSDSFGHSPFEMFRQMFGGHRRGGGRHGFGPF
                     GGFGGFGGGGPGGFGGFGDDAMPRGRQFSADDLQLLLLALLAEQPSHGYELIKALDTRS
                     SGFYAPSPGMVYPALTYLEELGYVTVQAEGNRKRYALADAGRAHLDAQRERVEMLFAKL
                     AHLGRKMEFMRRAFAGEAPAAPEDDTRGWLPEFVEARMALKQALFRRSGANADEQRRIA
                     AILRRAVADIEGRAQG"
     gene            3071..4477
                     /locus_tag="BG19_RS04935"
                     /old_locus_tag="BG19_4451"
     CDS             3071..4477
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009981062.1"
                     /locus_tag="BG19_RS04935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BG19_4451"
                     /product="cytochrome P450"
                     /protein_id="WP_044358391.1"
                     /sec_met_domain="p450 (E-value: 1.6e-34, bitscore: 116.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGLPLSLPRVDSTDSLFADPLAFLARARAQCGDVFVLREHGPIF
                     SRASDCSGVIAVFGEHRLRQVLTETDTFALPMSAAAKMALPKNLVNLNRGLHSMREPEH
                     GRHKRLLTGTANRALFDAHRSGMQTDLSRFCEMLNGDGRISVVSRMRELTAKLASRLFL
                     GPQCEEDAELTFLLSAYFTLRREASSPNAHDPLQYRDALIGAGRQLDRALRARIRRYRK
                     VPAGECAGLLQRLATAGQPGSPALSEDEIVGHANVMFVSSTEPVAISLAWLLLVLSQLP
                     DLRRALRAESAARASSPASPYDAPLLERVINETLRLLTPNALMVRATMQAVSLQGIALP
                     ARCEIVVCPFLVHREANAFARPHAFLPSRWETARPSPYEYFPFGAGGHFCAGRNLALSL
                     IGEVLSTLLSRFDFVLDIEQFIDWRIHIMLMPKGDPTLTAHPVDERRDTPSPKWRGPVA
                     ELFHFAPGLS"
     misc_feature    4532..4546
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.99"
                     /tool="antismash"
     gene            4627..4827
                     /locus_tag="BG19_RS04940"
                     /old_locus_tag="BG19_4452"
     CDS             4627..4827
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525608.1"
                     /locus_tag="BG19_RS04940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BG19_4452"
                     /product="hypothetical protein"
                     /protein_id="WP_004201048.1"
                     /transl_table=11
                     /translation="MATKPKKTKGVSVSIEGKLPKMTLDMPVDAKKIKAIQKCLENGKL
                     TITMSKVDLAGGRMGEGYLYD"
     misc_feature    complement(4641..4650)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.0"
                     /tool="antismash"
     gene            5001..5852
                     /locus_tag="BG19_RS04945"
                     /old_locus_tag="BG19_4453"
     CDS             5001..5852
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603954.1"
                     /locus_tag="BG19_RS04945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BG19_4453"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_004196528.1"
                     /sec_met_domain="DUF692 (E-value: 8.6e-35, bitscore: 117.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAFDRDAGAPRIGLAYGPGMAEFAARHAHLVDYIEVPFELLRFS
                     PTIAELQQTIPFVLHCASLSVAGFVPPDDSTVDAIERTAVRTGTPWIGEHLAYISADPI
                     GEALGGTHEPTSLSYTLCPQLSDETVRRVADNLAVLAPRFPVPLIVENSPQYFSIPGST
                     MGMTDFIRAIADRCDVGLLLDLSHFLITAYNTGADVHREFARLPLERVVEVHLSGMSIQ
                     SGAAWDDHSLPASPILFELLERLLAVARPRALTFEYNWSPYFPLSVLTSHIERARALMG
                     LA"
     gene            5849..6670
                     /locus_tag="BG19_RS04950"
                     /old_locus_tag="BG19_4454"
     CDS             5849..6670
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543057.1"
                     /locus_tag="BG19_RS04950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BG19_4454"
                     /product="hypothetical protein"
                     /protein_id="WP_045603842.1"
                     /transl_table=11
                     /translation="MNARTIHALIAASTKDPQLIERWRRHPDLLRGAGIAPGDLDWRAL
                     EHFVGLSLKVRHNGLRGDFPLSFRLLSVVGLEIEVFAAYASDCAESGHVFAATPQERAR
                     DLIAFLDRWLDPAQRNHVLLWDILRHEYALACLRQAGDAPLPDARHRARATAGGIPTLN
                     GSVVQHVMRCNPDDLAAALHQKEPPLDAVPAVDTYCCYWRSAETGEIRLLRVDTLGYFA
                     IEAIDGRRSAADICHRLLGNRRLLPQFLSSLDQLAGIGIVRFARPLGCASA"
     gene            6667..8610
                     /locus_tag="BG19_RS04955"
                     /old_locus_tag="BG19_4455"
     CDS             6667..8610
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004549365.1"
                     /locus_tag="BG19_RS04955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BG19_4455"
                     /product="radical SAM protein"
                     /protein_id="WP_004546215.1"
                     /sec_met_domain="PF04055 (E-value: 7.2e-14, bitscore: 49.8,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLLLVDNLVMPEEGSLAYLDVHPHLGLLALAAVAEADGHRVRIY
                     DPKRLIRDGTLAYDATLYERASRAILAERPDAVGFTALGCSFLFALNVAALLKRREPDL
                     PVLLGGPHATMLHRQILERFPQFDIIVRYEADEILPAVLDCLEQRTFDVIPGLSWRATG
                     RGAPLRFTDGKPKVEELDRLPIASYDHYPVDDLGLSMLRIEAGRGCPFACTFCSTAGFF
                     QRSFRLKSAERLVHELDILHRRYRVSDFKLDHDMFTVNRHKVMAFCEAVAGRGYRWRAS
                     ARIDCVDEALLRKMADAGCVNLYFGVETGSERMQTICRKRLDLRLVEPILAAADSFGIE
                     TTASFITGYPQESGQDQDDTLDMIGRCARRPSCLTQLHMLAPEPGTPLFDEHGAQIAYD
                     GYGGPYNTRLLGPEDEREVLDHPDIFQTYYYYPAALPRARHIFAVEAVDVLRRAGPIIL
                     AYALREFGGRLSRLISAWRRFADETSPGALPDAAGLEAFVAARFGRGHHLHSLFRYALR
                     LSAARAPADVVAEGPYEPDRPCMLAENVGLLEDIHNCEALVDRLRRSNDDAPALGDADV
                     GGLGAYIVRADGEAVTGYWVESGIAAILDLFRAPRSCRAAAQWLAHVTRNDGVDASIFE
                     PLLRRGILVPAR"
     gene            8624..9613
                     /locus_tag="BG19_RS04960"
                     /old_locus_tag="BG19_4456"
     CDS             8624..9613
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852411.1"
                     /locus_tag="BG19_RS04960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BG19_4456"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_004545057.1"
                     /transl_table=11
                     /translation="MARRKLERWSALDLAEGLQLAHAVAALQTLGVVDAMAEPTTAQTL
                     SAACDLDPELLRGVLEYAASRTNLIRKTGDRFAASEHYTAQARFLLNLYAGAYADNASR
                     LATLLRRPALAGTTVDLVAHARAFEASEAGGGALAAIISQLHLNHVLDLGCGAGTLLHA
                     LAADDREFVGWGLDRNPSMCKAARMRARQAGIAARVKVFQGDGRHPGASIPPRVLARVR
                     NVVASQFVNEMFRGGTSRAQAWLRRMRRLLPDRLLVIADYYGRLGHGFQTPHRETLLHD
                     YAQLISGQGVPPPNADAWLAMYRAAGCRPLHVIEDRAATTRFVHLVAL"
     gene            complement(9785..10405)
                     /locus_tag="BG19_RS04965"
                     /old_locus_tag="BG19_4457"
     CDS             complement(9785..10405)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894570.1"
                     /locus_tag="BG19_RS04965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BG19_4457"
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_004194492.1"
                     /transl_table=11
                     /translation="MNWIAFSHGAMLCASLIVTIGAQNAFILRQGIMRSHVGKIVLLCT
                     LSDMLLIGAGVGGASVLVERYPAFVHAVLYVGLAYLAWFGLSALRRAFKPGHETLDATG
                     AVAAPPAQRAWPIVLMTLAFTWLNPHVYLDTFLLIGTAGAREPEGARGAFAIGAMAVSA
                     VWFVALGYGARLLAPWFKRALAWRVLDGAIGGMVLFLAAVQLR"
ORIGIN
        1 gcgaatcggt gcacgcggtc gcgttcacgc gattgcacga gcggccgatc cgcatcgact
       61 gacgctgatc ggcaggaatc cggatcaacc cggcgttctc gcctgggcgg cgtcgttcgt
      121 cgagcccggc tcgacatcgg cgccgccgtg caattcgacg ttcccgttct gcaactggcg
      181 cgcggcttcg cgatcgagcg cgctgtcttt gaggatcgcg cacatcgcga cgaacagcgc
      241 gagcgcgatt gcggcgagcc cgcaatagcc gacgattttg agcgttctcg gaatgccggc
      301 gtgggtgtgg tggcgagtca tggacgcgta tccgggtggc gacgcgcgaa gccgcgcggt
      361 ctcgttctcg ttcgacacgc gaaaatacgc ggtgcgcgcc gtgcttttcc ctatccggtc
      421 acggcggcgc gcgccccgtt gtgccgccat cggcgtcgcc atgtgccgtc gacataggta
      481 taggacacgg gcgatgcgga tgcaactccg ctcgtgtgga gacgcgcgga tgcgagggca
      541 tcgcgcgatg caagcggacg atggaacctg aacgcgcgcg ccggccgccg tcgctgcggc
      601 gttgatcggc gggaaaacgg cggagaagcg aacgaaacgg ctttgtcggc gcgcggaatg
      661 gcagcgaagg cgtgcgatgc gatgggtcag cgcggcgcgg ctgcgctcga gcggcgcggc
      721 ggtgcggtgc gatcgggcgc gaaccagagc cgcttgccat gagcgacgta tcgcgagcgt
      781 ggcggtgcgc ggctatttca agacaatcaa gacaagccga tgcgatgcga cagcggtacg
      841 cgcgcgattc agctcatacg aaacggcgaa aacgcgacgc ggcgaacgta gcggcggcgt
      901 gaaggggttt gcggttttgg ctttcgcttt cggcgatggc cggtcgaggc gtacgcgcgt
      961 gtgctgcctg ccggccgcta tcgcgtgcat tgcaatactg catgcgggtg ccggcggcgc
     1021 cgtgcgcttg gatcgcgcgc gtgaatcgcc gggcgatatt cgcgcgcgca gcggccggcc
     1081 gcggatcagt cttcgagcga ttcgtgcacg gcgatcgcac cgtgcttcca gtaagcggat
     1141 gcgcgaatcc gcgatttcgc gacgccgcgc tcgcggcaca gatgctcgcg cacgccgcgc
     1201 atcgcggccg cctcgcccgc cgcccatacg tagccttcgc ccgccggcag cggcagttcg
     1261 cgcaatgcgt cgaccagccg gctcgcgccg cgctcgccgc tcgccgcatc gccgcgatgt
     1321 cgccatacca catacaggtt cgcatcggtt tcgaatgcga tctgcgccga cggatcggcc
     1381 acttcgagca ccgccgcgac gcgcgtgccg gccggcaatt cctcgagccg gcgcgcgacg
     1441 gcgggcaatg cggtgtcgtc gccgatcagc agatgccagt cgaaatcctt cgggatcacg
     1501 aacgaaccgc gcggcccgcc gatgccgagc gtttgcccga cgcgcgcctg agcggcccac
     1561 tgcgaagccg gccccggatg atgcagcacg aaatcgagat cgagctcgcg cgcggcgcga
     1621 tcatagcggc gcggcgtgaa atcgcgtgcg acggggcgcg gcctgtcctg cggaaacacg
     1681 atgccgttcg ggccgaattc ggggcgcgcg ggtgcgtctt cgccgggcgg cgggaagaag
     1741 actttcacgt gatcgtcgaa cgacgccgat tcgaaatcgt caagttcgtc gcctgccagc
     1801 gtcacgcgct tcagatgcgg cgtcacgtcg acgacgcgct tgacctgcag caggcggaat
     1861 ttcagcgtat gacgcacgcg tacgaccgcg cgctcggtag tcggttgggt catgtcgatc
     1921 ggttctcctt gatgagccgg aaacggcgcg attcagcctt gcgcgcggcc ttcgatgtcg
     1981 gcgacggcgc gccgcaaaat ggccgcgatg cggcgttgct cgtcggcgtt tgcgccgctt
     2041 ctgcgaaaga gcgcttgctt gagcgccatc cgtgcttcga cgaattccgg cagccagccg
     2101 cgcgtgtcgt cctcgggcgc ggcgggcgct tcgccggcga acgcgcgacg catgaattcc
     2161 atcttgcggc cgagatgcgc gagcttcgcg aacagcatct cgacgcgttc acgctgcgcg
     2221 tcgagatgcg cgcggcccgc gtcggcgagc gcgtaacgct tgcggttgcc ttcggcctgc
     2281 accgtcacgt agccgagctc ttccagatac gtgagcgccg gatacaccat gccggggctc
     2341 ggcgcgtaga agccgctcga gcgcgtgtcg agcgccttga tcagctcgta gccgtggctc
     2401 ggctgctcgg cgagcagcgc gagcagcagc aactgcagat cgtcggcgct gaattgacgg
     2461 ccgcgcggca tcgcgtcgtc tccgaagccg ccgaacccac cggggccgcc gccgccgaag
     2521 ccgccgaagc cgccgaatgg gccgaagcca tgccggccgc cgccgcggcg atggccgccg
     2581 aacatctgac gaaacatctc gaacgggctg tgtccgaacg aatcggacat gccgcattcg
     2641 cggcgcgaaa aacggtgagc gtgacgcatg tccgcctcct gtgtctttag atgtatctaa
     2701 agatatatat cgaaagatat aaagtcaacg gaatttaaac ggggaacgat ggggcggagt
     2761 gcgatggccg gacgggcgat gtcccgcctg gtaccgacac gcggagggcg cgacgatgcc
     2821 ggaatgccgc gtgcaagggt ggggcgacgg ccgaaggcgg catggcgaac ccggcctctg
     2881 gcccgttcgt tcgatggcgg atgtttatcc gtcccggggc gtgccgtatt gcgcggcggc
     2941 gttgcgatcg cgctcgggcg gtaccggccg ggcggcttcg gaagacgtga tactgtgacg
     3001 gcagccatgc tgtagcgatc aaccggcgta gccacgatcg gcacgaatcg ttcacggaca
     3061 cagcgcgcaa atgaatggcc tccctctgtc gcttccgcga gtcgacagca ccgattcgct
     3121 ctttgcggat ccgctcgctt ttctcgcacg agcgcgcgcg caatgcggcg acgtcttcgt
     3181 gctgcgggag cacggtccga ttttttctcg cgcgagcgat tgcagcggcg tgatcgccgt
     3241 gttcggcgaa catcggcttc gacaagtctt gaccgagacg gacaccttcg cactgccgat
     3301 gtcagcggcg gcgaagatgg cgttgccgaa gaatctggtc aacctcaacc gcggcttgca
     3361 cagcatgcgc gagccggagc acggccggca caagcgcctc ctgacgggaa cggccaaccg
     3421 cgcgctgttc gacgcgcatc gatccggaat gcaaacggat ttgagccgct tttgcgaaat
     3481 gctgaacggg gacggccgga tttccgtcgt gagccgaatg cgcgagctga cagccaagtt
     3541 ggcgtcccgt ctttttcttg ggccgcagtg tgaggaggat gccgaactga cgtttctgct
     3601 aagcgcgtat ttcacgttgc ggcgcgaggc gtcttcgccc aacgcgcacg atccgttgca
     3661 gtaccgggac gcgctgatcg gcgccgggcg gcaactcgat cgcgcgttgc gggcgcggat
     3721 ccggcggtat cgaaaggtgc ccgccggcga gtgcgcgggg ctgctccagc ggctggcgac
     3781 ggccggccag ccgggttcgc ccgcgctttc cgaggatgag atcgtcggcc acgccaacgt
     3841 gatgttcgtg tcgagcacgg agccggtggc gatatcgctg gcgtggctgc tgctcgttct
     3901 gtcgcagttg cccgatctgc ggcgcgcgct tcgcgcggaa agcgccgccc gcgcatcgtc
     3961 gccggcttcg ccgtacgacg cgccattgct cgagcgcgtc atcaacgaga cgcttcggct
     4021 cctgacgccc aacgcgctga tggtccgcgc cacgatgcag gcggtttcgc tgcaaggcat
     4081 cgcgcttccc gcgcgctgcg agattgtcgt gtgcccgttc ctcgtgcacc gcgaggccaa
     4141 cgcgtttgcg cgcccgcatg cattcttgcc gtcccgatgg gagacggcga ggccgtcgcc
     4201 gtacgagtat tttccattcg gcgccggcgg ccatttctgc gcggggcgga atctcgcgct
     4261 gtcgctgatt ggcgaagtgc tgtcgacgct gctatcgcga ttcgatttcg ttttggatat
     4321 cgaacagttt atcgactggc gcattcatat catgctgatg ccgaaaggcg acccgacgct
     4381 cacggcgcac cccgtcgacg aacgccgcga cacgccgtcg ccgaaatggc gcgggcctgt
     4441 cgccgagctg ttccatttcg cgccggggct ttcttgacat ttaccggggc gcatccttta
     4501 atcagaagca aatcggcatg cacacccgac gggatttttt cggtcatcga ttcgcatggc
     4561 gattttcggg ggttattccg cactcacagc tgagcgtcgc gtcctgccca caacaaagga
     4621 acgatcatgg ccacgaaacc caaaaaaaca aagggcgttt ccgtttccat cgaagggaag
     4681 ctgcccaaga tgacgctgga tatgccggtg gacgcgaaaa agatcaaggc catccagaaa
     4741 tgcctggaaa acggcaagct gacgatcacg atgagcaagg tggatctcgc cggcggccgc
     4801 atgggcgagg gctatctcta cgactgacgt ccggcggcgc gcgcgggcct tgccggccgc
     4861 gccgcattct cgtcgcctcg atgctcgggt ttcgcagaga agccgcgccg ccgaacgcga
     4921 ggcgttcggc gtgcgctcgc gcaatggcgg gcgataaatc tggactgctg cggttcggcg
     4981 cctgaaggcg cgggaagcct atgaaagcgt tcgatcgcga tgccggggcg ccccggatcg
     5041 gccttgcgta cgggcccggc atggcggaat tcgccgcacg gcatgcgcat ctcgtcgatt
     5101 acatcgaagt gccgttcgag ctcttgcgat tctcgccgac gattgccgag cttcagcaaa
     5161 ccattccgtt cgttctgcac tgcgccagct tgtccgttgc gggattcgtg ccgcccgacg
     5221 attcgaccgt cgacgcaatc gagcgcacgg ccgtgcgcac cggcacgccg tggatcggcg
     5281 aacatctcgc gtacatctcg gccgatccca tcggcgaggc gctgggtggg acccatgagc
     5341 ccacgtcgct gtcttacacg ctgtgccctc agttgagcga cgagacggtg cggcgcgtcg
     5401 ccgataatct cgccgtgctc gctccgcgct ttccggttcc gctgatcgtg gaaaattcgc
     5461 cccaatactt ttcgattccc ggcagcacga tgggcatgac cgacttcatt cgcgcgatcg
     5521 cggaccggtg cgatgtcggc ttgctcctgg atctgagcca ttttctgatc acggcgtaca
     5581 acacgggcgc cgacgtgcac cgggaattcg cccggctgcc gctcgagcgc gtggtcgaag
     5641 ttcacctgtc cggcatgagc atccagtccg gcgcggcctg ggacgatcat tcgctgccgg
     5701 cgtcgccgat cctcttcgaa ctgctcgagc gcctgctcgc cgtcgcgcgg cctcgcgcgc
     5761 tgaccttcga atacaactgg tcgccatact tcccgttgtc ggtcctcacg agccacatcg
     5821 aacgcgcgcg cgcgctgatg gggcttgcat gaacgcaagg acgatacacg cgctgatcgc
     5881 ggccagcacc aaggatccgc agctcatcga gcgttggcgg cggcatccgg acctgctgcg
     5941 cggcgcgggc atcgcgcccg gcgatctcga ttggcgcgca ttggagcatt tcgtcgggtt
     6001 gagcctgaag gtgcgccaca acggcctgcg cggagatttt ccgctgagct tcaggctgct
     6061 gagcgtggtc ggcctcgaga tcgaggtatt cgccgcgtat gcgtcggact gcgcggagag
     6121 cggccacgta ttcgccgcca cgccgcagga gcgcgcgcgc gatctgattg cgtttctcga
     6181 tagatggctc gatcccgcgc aacgcaatca cgttctgctg tgggacatcc tgcgccatga
     6241 atacgcgctc gcatgcctgc ggcaagcggg cgacgcgccg cttcccgatg cgcgtcatcg
     6301 ggcgcgcgcg acggccggcg gcattccgac gctcaacggc tccgtcgttc aacacgtgat
     6361 gcgctgcaat cctgacgatc tggcggcggc gcttcatcag aaggagccgc cgctcgacgc
     6421 ggttcccgcc gtcgacactt actgctgcta ttggcgctcc gccgaaacgg gcgaaatacg
     6481 gttgctgcgg gtcgatacgc tcggttactt cgcgatcgaa gcgatcgacg gcaggcggtc
     6541 cgccgccgac atctgccatc ggttgctcgg caatcgccgt ctgctgccgc agttcctgag
     6601 ttcgctcgac caactggccg gcatcgggat cgtgcgattc gcgcggccgc tcggatgcgc
     6661 ctcggcatga agctgctgct cgtcgacaat ctcgtcatgc cggaagaggg ctcgctcgcc
     6721 tatctcgacg tgcatccgca tctcgggctg ctcgcgctgg ccgcggtcgc cgaagccgac
     6781 ggccatcgcg tgcggatcta tgatccgaag cgactgatcc gggacggcac gctcgcttac
     6841 gatgcgacgc tttacgagcg ggcgagccgc gcgatcctcg ccgagcgccc ggacgcggtc
     6901 ggcttcaccg cgctcggctg cagttttctg ttcgcgctca atgtcgcggc gctgctgaag
     6961 cggcgcgagc ccgatttgcc ggtcctgctc ggcggcccgc acgcgacgat gctgcatcgg
     7021 cagatcctcg agcgattccc gcagttcgac atcatcgttc gctacgaagc ggacgagatc
     7081 cttcccgccg tgctcgactg cctcgagcag cggacattcg atgttatccc cggcttgagc
     7141 tggcgcgcga cgggccgcgg tgcgccgttg cgcttcaccg acggcaagcc caaggtcgag
     7201 gaactggacc ggctgccgat cgcgtcgtac gaccattatc cggtcgacga tctgggcctc
     7261 tcgatgctgc gcatcgaggc cggccgcggc tgtccgttcg catgcacgtt ctgttcgacg
     7321 gcgggcttct ttcagcgcag ctttcgcctc aagtccgccg agcgcctggt gcacgagctg
     7381 gacatccttc atcggcgcta tcgcgtgtcc gacttcaagc tcgaccatga catgtttacc
     7441 gtgaatcgcc acaaggtgat ggcgttttgc gaggcggtgg ccgggcgcgg ctaccggtgg
     7501 cgcgcgtcgg cgcggatcga ttgcgtcgac gaagcattgc tgaggaagat ggccgacgcg
     7561 ggctgcgtga atctttattt cggcgtggag accggctccg aacgcatgca gacaatctgc
     7621 cggaagcggt tggacttgcg gttggtcgag cccattctgg ccgcggcgga ttcgttcgga
     7681 atcgagacga ccgcgtcgtt catcaccggc tatccgcagg aaagcgggca ggaccaggac
     7741 gacacgctgg acatgatcgg gcgctgcgcg cggcggccgt cctgcctgac gcaactgcac
     7801 atgctcgcgc cggagcccgg cacgccgctg ttcgacgaac acggggcgca gatcgcctac
     7861 gacggctatg gcgggcctta caacacacgc ctgctgggcc cggaggacga gcgcgaggtg
     7921 ctcgaccatc ccgacatatt tcaaacctac tattactatc ccgccgcgct gccgcgcgcg
     7981 agacacatct ttgccgtcga ggcggtggat gtgctgcgcc gcgcggggcc gatcatcctc
     8041 gcgtacgctt tgcgggaatt cggcggacgg ctgagccggc tgatatcggc atggcgacgg
     8101 ttcgcggacg aaacgtcgcc cggcgcgttg ccggatgcgg cggggctgga ggctttcgtg
     8161 gccgcgcgat tcggccgcgg ccatcacctg cattctcttt tccggtacgc gttgcggctg
     8221 tccgcggccc gcgctccggc ggacgtcgtc gccgaagggc cctatgagcc ggaccggcct
     8281 tgcatgctgg ccgaaaacgt cggcctgctg gaggacatcc acaattgcga ggcgttggtc
     8341 gatcggctcc ggcgctcgaa cgacgatgcg ccggcgctcg gcgacgcgga tgtcggcggg
     8401 ctcggcgcct atatcgtccg cgcggacggc gaagccgtca ccggctattg ggtggagtcc
     8461 ggcattgccg cgatcctcga tctgttcagg gcgccccgtt cgtgccgcgc cgctgcgcaa
     8521 tggctggcgc acgtcacccg caatgacggc gtggacgctt cgatcttcga gccgctgctg
     8581 cgcagaggca ttttggtgcc ggcccgctaa attcatttcg ctcatggctc gtcggaaact
     8641 cgaacgctgg tccgcgctgg atctcgcgga aggtcttcaa ctggcgcatg ccgtcgccgc
     8701 gctgcaaacg ctcggcgtcg tggatgcgat ggcggagccg acgacggcgc aaacgctgag
     8761 cgccgcgtgc gatctcgatc ccgagttgtt gcgaggcgtc ctcgaatacg cggcatcgag
     8821 aacgaacctg atccgcaaga ccggcgaccg attcgcggct tccgagcact acacggcgca
     8881 agcgcgtttc ctgctgaacc tgtatgccgg cgcctatgcc gataacgctt cgcggctggc
     8941 gacgctgctc cggcggccgg cgctggcggg cacgacggtc gacctcgtcg cgcacgcgcg
     9001 cgccttcgag gcgagcgagg cgggcggcgg ggcgctcgcc gcgatcatca gccaattgca
     9061 cctgaatcac gtactggatc tcggatgcgg cgccggtacg ctgctgcacg cgctggcggc
     9121 ggacgatcgt gaattcgtcg ggtgggggct ggatcgcaat ccgtcgatgt gcaaggccgc
     9181 ccggatgcgg gcgaggcaag cgggaatcgc ggcgcgcgtg aaggtatttc agggcgacgg
     9241 caggcatccc ggcgcgtcga ttccgccgcg cgtgctggcg cgcgtgcgca acgtcgtcgc
     9301 gagccagttc gtcaacgaga tgtttcgcgg cggcacgtcc cgcgcgcagg cctggcttcg
     9361 acgcatgcgc cgcctgctgc ccgaccgcct gctcgtgatc gcggactact acggccgcct
     9421 cggccacggt tttcaaacgc cgcaccgcga gacgctgctg cacgactatg cgcagctcat
     9481 atcggggcaa ggcgtgccgc cgcccaatgc cgacgcgtgg ctcgccatgt atcgggcggc
     9541 cggttgccgg cctctgcatg tgatcgaaga ccgcgcggcc acgacgcgct tcgtccacct
     9601 ggtagcgctg tagcggcatg acgggcgcgg ctcggcggcg ccgcttgcgc cgaccgcgtg
     9661 cgccggctgg ccgattccct cagggcgtgc ggccatgacg ttcgtggctc gctcgccgcc
     9721 gtgcgccgcg tgccgccgtc cgcgcttcag atggcgtcgg caccgccaac gcgcatccgc
     9781 cgcgttagcg caactgcacc gccgcgagaa acagcaccat gccgccgatc gcgccgtcga
     9841 gcacgcgcca cgcgagcgcg cgcttgaacc atggcgcgag caatcgcgcg ccgtatccga
     9901 gcgcgacgaa ccataccgcg ctgaccgcca tcgcgccgat cgcgaacgcg ccgcgcgcgc
     9961 cttccggctc gcgcgcgccg gccgtgccga tcagcaggaa cgtgtccaga tagacgtgcg
    10021 ggttgagcca tgtgaacgcg agcgtcatca gcacgatcgg ccaggcgcgc tgcgcgggcg
    10081 gcgcggcaac cgcgcccgtc gcatcgagcg tttcgtggcc gggcttgaac gcgcggcgca
    10141 gcgcgctcag gccgaaccat gcgagatagg cgaggccgac atacagcacg gcatgaacga
    10201 aggccggata gcgctcgacg agcaccgatg cgccgccgac gcccgcgccg atcaacagca
    10261 tgtccgacag cgtgcagagc aggacgatct tgccgacgtg cgaacgcatg atgccctgcc
    10321 ggaggatgaa cgcattctgc gcgccgatgg tgacgatgag cgatgcgcag agcatcgcgc
    10381 cgtgagaaaa agcgatccag ttcatgttcg ttgcggcttc cggcggtgga ctaacggggc
    10441 aagaggcgct agtctgcgat gatcgcttga gtaagaaaag ctaaatgatc taatgccaat
    10501 taaggaaaac tgatgctcga ctatgcactg ctcgatgcgc ttgccgaagt gatccgccac
    10561 ggctcgttcg agcgggcggc gaaagcgctc aacgtgtcgc cttcggcggt gtcgcagcgc
    10621 gtgaagctct tggaggagcg cgtgggcagc gtgctcgtga agcgcgggca gccgtgcgtc
    10681 gcgacgacct cgggcgcgct gctgtgccgc cacaccgagc gcgtgcggat gctggaggcc
    10741 gagctgtccg ggcacatgcc cgcgttcgcg ggcgcgcaag cgggggcgtg gccgacgctg
    10801 cgcgtggccg tcaacgacga cagcgtcgcg acctggttcg tcgacgcggt tg
//
