LOCUS       NZ_CP059734            10987 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Thalassomonas viridans strain XOM25 chromosome pTvir, complete
            sequence.
ACCESSION   NZ_CP059734
VERSION     NZ_CP059734.1
KEYWORDS    RefSeq.
SOURCE      Thalassomonas viridans
  ORGANISM  Thalassomonas viridans
            Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
            Colwelliaceae; Thalassomonas.
REFERENCE   1  (bases 1 to 1218661)
  AUTHORS   Pheiffer,F., Schneider,Y.K., Hansen,E.H., Andersen,J.H.,
            Isaksson,J., Busche,T., Ruckert,C., Kalinowski,J., Zyl,L.V. and
            Trindade,M.
  TITLE     Bioassay-Guided Fractionation Leads to the Detection of Cholic Acid
            Generated by the Rare Thalassomonas sp
  JOURNAL   Mar Drugs 21 (1), 2 (2022)
   PUBMED   36662175
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218661)
  AUTHORS   Olonade,I., van Zyl,L.J. and Trindade,M.
  TITLE     Draft Genome Sequences of Marine Isolates of Thalassomonas viridans
            and Thalassomonas actiniarum
  JOURNAL   Genome Announc 3 (2), e00297-15 (2015)
   PUBMED   25883295
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1218661)
  AUTHORS   van Zyl,L.J., Busche,T., Ruckert,C., Kalinowski,J. and Trindade,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2020) IMBM, University of the Western Cape, Robert
            Sobukwe, Cape Town, Western Cape 7550, South Africa
REFERENCE   4  (bases 1 to 1218661)
  AUTHORS   Olonade,I., van Zyl,L.J. and Trindade,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2015) IMBM, University of the Western Cape, Robert
            Sobukwe, Cape Town, Western Cape 7550, South Africa
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8; Canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 107.2x; 129.1x
            Sequencing Technology             :: ONT; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000948985.2-RS_2024_03_26
            Annotation Date                   :: 03/26/2024 01:21:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,855
            CDSs (total)                      :: 6,705
            Genes (coding)                    :: 6,644
            CDSs (with protein)               :: 6,644
            Genes (RNA)                       :: 150
            rRNAs                             :: 11, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 117
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 44 of 61
            Pseudo Genes (internal stop)      :: 12 of 61
            Pseudo Genes (multiple problems)  :: 18 of 61
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:09:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 743204
            Orig. end                         :: 754191
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059734.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10987
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10987
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10987
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5987](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5987)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(261..1028)
                     /locus_tag="SG34_RS32080"
                     /old_locus_tag="SG34_031980"
     CDS             complement(261..1028)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SG34_RS32080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SG34_031980"
                     /product="hypothetical protein"
                     /protein_id="WP_044839050.1"
                     /transl_table=11
                     /translation="MFELQKKGKRNFHNLTNRTLGRVNAIPHISKSRVAIHNILNDSSL
                     PMDGEKNAARYRTRGVKKPTKADSIAQPFGGAILTSSDKIGREEPDGQDQNASLTSMTT
                     FTKSPTKKGLSIEVEGENLFSSSDYPGGFRWTQTIDTNAPLGGTTTPYVDPRPNDDSKP
                     FYWTDAEHASSPSTFKDKPWRPVPASGTTWWTATLALNGVDDAKKKVTAFDAMSYGFTL
                     DSSGTVTLVEPTSVSLTGHQSVLSSEFSSWTFS"
     gene            1338..1739
                     /locus_tag="SG34_RS32085"
                     /old_locus_tag="SG34_031985"
     CDS             1338..1739
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /locus_tag="SG34_RS32085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_031985"
                     /product="hypothetical protein"
                     /protein_id="WP_053046695.1"
                     /transl_table=11
                     /translation="MAVVPYLCICACWPMWQLASANYIEHYGLQRQQIPEGRYEQIQLH
                     HSWNSDHIFSNWALFHLQRHSDHYANAARSYQVLRHFNGLPTLPSGYFGMYILAYLPRL
                     WFKVMDKCLLQVTGGGISVAGRVKSFILQ"
     misc_feature    complement(2089..2102)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.97"
                     /tool="antismash"
     misc_feature    complement(2090..2103)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.08"
                     /tool="antismash"
     misc_feature    complement(2096..2105)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    complement(2566..2579)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.15"
                     /tool="antismash"
     misc_feature    complement(2583..2596)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.49"
                     /tool="antismash"
     misc_feature    complement(2601..2614)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.18"
                     /tool="antismash"
     misc_feature    complement(2602..2615)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.86"
                     /tool="antismash"
     misc_feature    2603..2616
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.56"
                     /tool="antismash"
     misc_feature    2922..2936
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.24"
                     /tool="antismash"
     gene            3193..3591
                     /locus_tag="SG34_RS32090"
                     /old_locus_tag="SG34_031990"
     CDS             3193..3591
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SG34_RS32090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SG34_031990"
                     /product="hypothetical protein"
                     /protein_id="WP_152647442.1"
                     /transl_table=11
                     /translation="MSASNTPGNSVSRNIIMLLVSITIAAFIWLMYGSDKTSMQAINNM
                     QDAEYLQQLAICDVQPTILTSEPLSVCAVAYQKLNFVKQGDEVCVEQTSGGPRCFSVHK
                     TKQIMQSINKKTDFEMNFDTWFAGLKEI"
     gene            complement(3657..4094)
                     /locus_tag="SG34_RS32095"
                     /old_locus_tag="SG34_031995"
     CDS             complement(3657..4094)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011041658.1"
                     /locus_tag="SG34_RS32095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_031995"
                     /product="DoxX family protein"
                     /protein_id="WP_044841919.1"
                     /transl_table=11
                     /translation="MTLSEVRLLHNRILDFSHYLLSPANVLVRLYIASIFFKSGLTKLR
                     DWESTLMLFEYEYEVPLLSPAAAAWSGTIGELVLPVLLAFGLFTRLSALGLFVVNYVAV
                     LSLVDISPAALNQHILWGSLLLMLLLIGGERFSLDRKLKIS"
     gene            complement(4178..5011)
                     /locus_tag="SG34_RS32100"
                     /old_locus_tag="SG34_032000"
     CDS             complement(4178..5011)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /locus_tag="SG34_RS32100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_032000"
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="WP_044841920.1"
                     /transl_table=11
                     /translation="MTLEHQQSALLEAIFSQPGPGEPLTAKGLAVYQKSLLHNASFALK
                     ITFATVHSFVGEAVFQELVAGYLQTGLKSEYDWGEFGLDFPQFIAGQAINHAALLGSLA
                     GLDYACHQSERSEDASRDLESFNLLAGQDPYLLSLSLSAGTRVLSSAFPLDAIITDMQK
                     LNTEETPLAFSEIDKQLSYYSQKYPRKQQDNSKVNADDEQFYYLVWRPHFQAQYTRITA
                     AEYQWLQLWLSAEDDPALSLGQALDKAGEDFSIVDWLPKAIEQQLLNAVYLKQTL"
     gene            complement(5001..5987)
                     /locus_tag="SG34_RS32105"
                     /old_locus_tag="SG34_032005"
     CDS             complement(5001..5987)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006013039.1"
                     /locus_tag="SG34_RS32105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_032005"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_063890926.1"
                     /sec_met_domain="DUF692 (E-value: 4.4e-69, bitscore: 228.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFPTQVKTPANLPKVGVGLRHAHYQDALDTPANIDFIELHAENFF
                     AAGGVSRALLADIRQHYDISLHATSLGLGSALPAPQAQIQQLADLIRICQPIMVSDHAC
                     FSWAEVNGRPVHGGDLLPVPFNDETLEIMAANVSRVQALLKRPILVENLSAYLILPGST
                     YSESEFLVRLCRLTGCKLLIDINNLVVNAQNHALLQPDSAKLDVIAYTQQWLNTIPQDY
                     VGELHLAGFTPTGEGELVIDDHSQAVSDEVWSLYRFALERFGAVATLIEWDNQLPSWQV
                     LVAEAAKAKAIAAEVFASAYQPDSQQPMTSHHQAVHKVIKEAANHDA"
     gene            complement(6019..6315)
                     /locus_tag="SG34_RS32110"
                     /old_locus_tag="SG34_032010"
     CDS             complement(6019..6315)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014109562.1"
                     /locus_tag="SG34_RS32110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_032010"
                     /product="DUF2282 domain-containing protein"
                     /protein_id="WP_044841921.1"
                     /transl_table=11
                     /translation="MSHKLAVSTALAAALALGALSDAEAAEKRVKKEKCYGISPAGQND
                     CSNLAGTHSCAGQSTVDNDPGEWRLVTKGLCKSQGGYTRKEAREIIKQLKSAK"
     gene            complement(6370..6837)
                     /locus_tag="SG34_RS32115"
                     /old_locus_tag="SG34_032015"
     CDS             complement(6370..6837)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011041663.1"
                     /locus_tag="SG34_RS32115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_032015"
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="WP_044841922.1"
                     /transl_table=11
                     /translation="MFTTTLKSVLAGSLLAASSLSFAADIDMNADVNDLAIKGYDPVSY
                     FTMAAPKLGNVNYTATYKGGIYRFSNEENRDLFNKNPAKYAPQYGGYCAFGVAMEKKFD
                     TDPLAWKIADGKLYLNLNKDVQKKWLTDVPGYVSQSDENWQDIRKVPAAEL"
     gene            6947..7768
                     /locus_tag="SG34_RS32120"
                     /old_locus_tag="SG34_032020"
     CDS             6947..7768
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /locus_tag="SG34_RS32120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_032020"
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_044841923.1"
                     /transl_table=11
                     /translation="MDHLSQIIDHFKLTAGVFYTGKLCGLSHFNENEALEGHIHLLRSG
                     VLAINGLDKSPLVLDQPSIVFLPRPTKHSLKAREVDNAELICANITYGSSSTNLLANAL
                     PDMLILPLESSPFLQTNIDWIIAEAGGDQEGRQAVMNRLMEIFIINMLRSLLVKGELAR
                     GMLAGLMHPQLKQVLQQLHNAPQENWSLNKMAELALMSRSKFAEVFKSVIGQTPNDYLL
                     SWRVATAQSLLKQGKSVTLVANSVGYDTASALSRVFRKTCGMSPKQWLDTL"
     misc_feature    complement(8241..8254)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.49"
                     /tool="antismash"
     gene            8372..8731
                     /locus_tag="SG34_RS32125"
                     /old_locus_tag="SG34_032025"
     CDS             8372..8731
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SG34_RS32125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SG34_032025"
                     /product="hypothetical protein"
                     /protein_id="WP_044841924.1"
                     /transl_table=11
                     /translation="MSEYTDNPADNSDLESSAPEEEENQTAEHSDEQKPIASELLALTD
                     DFAEFSADCAFLCDAFAVIAEEQEDISDYTSYGVRRCSDYTSYGVRRCSDSVKEQVIAL
                     DRRIHALQRRVSEQD"
     gene            9139..10065
                     /locus_tag="SG34_RS32130"
                     /old_locus_tag="SG34_032030"
     CDS             9139..10065
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SG34_RS32130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SG34_032030"
                     /product="hypothetical protein"
                     /protein_id="WP_152647443.1"
                     /transl_table=11
                     /translation="MRFLVQVFIGIAFGLISQDARTSTSPCYSPFPELTATTQKPVLSA
                     GYGAFDPADTSLAGFSEQSTIFTDLNVFLPGSKSGNMQNYWQLSHHYRAFDFEEPAGTQ
                     LHSNGDHHKLALLWHGLNRQKQHQWAFGISPLLSVTSNQLKNTGRLNSNAWQLHGFIEY
                     RQFYSKKWQWLLGACMDDRFDHVKAYPIAGVAGDINERTRLRLAFPDTKLTVKLTPHWH
                     VSTLLSPAGSKWDAFNKTADKHSDFHYRSWRFSVAARWQFNSQWHFRFEWGREFDRRFR
                     FRQLAGNQVSTVAESVNYVAAYIIWYW"
ORIGIN
        1 gcaatatcac cgccaacttg aattgaaggc ttaaaaactt ctgttgtgtg atattctcgt
       61 tgcagttccc ataactgctc tagtagttat gcactaaaat ctcgtcggat ttcagtatta
      121 tgaagtctgg cgaggtaatg ttctttttat tcttttgttt tttcgtaact ttttgggcat
      181 tttatacata taaacgttat aaaaaaactt ggacaaccag tgccggagta agccttgcta
      241 gttgtaataa tcccctttcg ctagctaaaa gtccatgatg aaaactcaga agacaaaaca
      301 ctttggtgac ccgtcaagga gacggatgta ggttcaacaa gcgtgacggt accagaacta
      361 tccagggtaa aaccataaga catcgcatca aatgctgtaa ctttcttttt agcatcatct
      421 acgccattta aagctaaagt tgcagtccac caggttgtcc cggatgctgg caccggtcgc
      481 catggtttat ctttaaatgt agaagggctg ctggcatgct ccgcatcggt ccaataaaag
      541 ggtttgctat catcatttgg ccgtgggtcc acataagggg ttgtagtacc acctaatggc
      601 gcattggtat caattgtttg cgtccaacga aaacctcccg ggtaatcaga ggatgaaaaa
      661 aggttttctc cctctacttc tatcgacaat ccttttttag ttggcgactt ggtaaatgtt
      721 gtcatactgg tcagtgatgc attttgatct tgcccgtcag gctcttccct gccaatttta
      781 tcagagctag tcagtatcgc ccctccgaaa ggctgagcaa tggaatcagc cttggtaggc
      841 tttttaacac cgcgggttcg atatcttgct gcgttcttct ccccatccat cggcaagctg
      901 ctatcattga ggatattgtg aatcgcaaca cggcttttac taatatgcgg aatagcattt
      961 actctaccca gagtacgatt agttaggttg tgaaagttcc tttttccttt cttctgtaac
     1021 tcaaacatgc tttgctcctc tatcaacata gagacttttc atatgtaact atagttaatc
     1081 gatagagtgg gataaataat atattgatgt actttttata aagcatggat ttgatatttg
     1141 aatagcttct tttttatttg agacgtgatt gccgccgatg tctgtgtagt gaaggaatat
     1201 atctgtattg agtaacgtct ggaaagtgtt tgaaatcacg acaaagcagg taagccgctg
     1261 tagttgccct aaaataaata tctgcaggct atgtatgtga ccctggtttt ttcactgcgc
     1321 tggtagtgtg gctcggcatg gctgttgtgc cttatttatg catttgtgcc tgttggccga
     1381 tgtggcaact ggcatctgcc aattatatcg agcattacgg cttgcagaga caacaaatcc
     1441 cggaagggcg gtacgagcag atacagctgc atcacagctg gaacagcgat catatctttt
     1501 caaactgggc gctgttccat ttgcagcgtc attcagatca ttatgccaac gccgcgcgca
     1561 gttatcaggt gttgcgccat tttaatggac tgcctacttt gccaagtggg tatttcggca
     1621 tgtatatcct ggcttatctg cccaggttat ggttcaaggt gatggataag tgcctgctgc
     1681 aagtaaccgg cggtggtatc agtgtggctg ggagggtcaa gtctttcatt ttgcaataac
     1741 tttaaattca tttattaaat cattgaaaag agaatcattg ataactcctc ctgcttagct
     1801 gcttagctgc ttagctgctt agctgctttg tgcaaaaagg atttactttg caaaaaacaa
     1861 aatttgaccc cacagcccga gagctattga gtatttcttt atttgggaga ctctaggtcc
     1921 aatttttaaa aagttaacaa caaaactttg ttttgggttt cctatatatg ggtggtttca
     1981 atcaacctat atttcatgaa acttcacccg ggacgagcaa tagtgatttc actgttgctc
     2041 gttttttgta tcatctgctc tttctcgggt gccgagcaaa taaagtactt ttatttaaaa
     2101 aaacactgaa agttataaac aaaaattacc ctgttgtttc ttagcgttga tggacgagtt
     2161 ttgtatcaat taataaatcc tgaaactata atgcaatttt gccagtaaca gcctgcgtgc
     2221 ttgttactgg tttttttatt ttacactcag ttagagtgcc ttagccgttt agctataaac
     2281 aaaaagttat ttagcgtgtc atactatgta attagttttt ttaagactaa ttttcatgaa
     2341 actggagctt gagccaataa caactacggt ggttgttatt ggttttttta tttcttctaa
     2401 ccttcttaga gcctgttcaa gtatagtttt tagtcttttc aaaaaaatca taaacaaaaa
     2461 tttattaact gtttcttaga aatgagaagt ttttgtatta accaacaaat cctgaaacta
     2521 aaatgcattt tcgccagtaa cagccaacgt gtttgttact ggctttttta tttttaaatt
     2581 catttaatta aaaaaatcca gtttttttaa aaaaaactat aaacaaaaac ttttttgccg
     2641 tttctcatgg atgattagtt cttgtaagac taacgacttc taacttcgat tgagcagatt
     2701 tagccagtaa cagtccatgg ggggttactg gtttttttat tttattctca gttacagtgc
     2761 tctaattaat tttcggttgt tctttatata tcttttgtta taacttgaaa gttctttaaa
     2821 tcaatttgtt gttgatggca gattgatgtt tgatgatgct aatctgggta agctgtatgg
     2881 agaaatgcaa tatgaataaa agtattgaaa agtcttgatc gagttttcaa ataattcaaa
     2941 ttttcatatg accttcaaaa gaaaactctt ccttatgttt aatgtgaaaa aatgagtaaa
     3001 gaggtgcatg atagtggcaa gcaacaaata gtattgacca ttcggaagtt ggcaattttt
     3061 tgtgctgcag gcgttcccta gtccatgacc tgagttattt ctttttataa ttatatgaat
     3121 ttatgagata ttatcttccc gtaaaaatct ttataatgct caaagcgtgc aagccagtta
     3181 aaaaggaaaa ctatgtctgc ttcaaacacc cccggtaact cagtcagccg aaatattatt
     3241 atgctattgg taagtattac tatcgccgct tttatttggc tgatgtacgg gtctgataaa
     3301 acatctatgc aagcaataaa taacatgcag gatgccgaat atctccagca gctggccatt
     3361 tgcgacgtac agccaacaat tttaacttct gagcctttgt cggtatgtgc cgttgcttac
     3421 cagaagctga attttgtgaa acagggggat gaagtttgtg ttgagcaaac ttcgggcggc
     3481 cccaggtgtt tttcggttca taaaaccaag caaatcatgc agtccatcaa taaaaaaacg
     3541 gattttgaga tgaactttga tacctggttt gccggtttaa aagaaatcta aaaatagggg
     3601 caccaccagt tagtgggtaa gcctgcacaa atgctggctt acccgctttt aatccgttag
     3661 gatattttca gtttacggtc caggctgaaa cgttcgccgc caatcagcag taacatcaac
     3721 aataaactgc cccataaaat atgctggttt aaggctgccg ggctgatatc gactaaactc
     3781 aataccgcca cataattaac cacaaacaag cccagagccg ataagcgggt gaataaacca
     3841 aaagctaaca ataccggtaa taccaattcg cctatagtgc ccgaccaggc ggcggctgcc
     3901 ggcgataaca agggaacttc atactcatat tcgaacagca ttaaagtaga ctcccaatcg
     3961 cgtagcttgg tcaggcccga tttaaagaaa atgctggcga tataaagccg gaccaacacg
     4021 ttcgcgggtg aaagcagata gtggctgaaa tctaaaattc ggttgtgtaa taacctgact
     4081 tctgagagtg tcataataat agctcctgta gtttacgcgg gggttttgtc ggcgcgttaa
     4141 ggtggattaa ttgggcttcg gttaatggtc aaagccgtta aagcgtctgt tttaaataaa
     4201 cggcgttaag cagctgttgc tctatggcct ttggcagcca gtcgacaatc gagaaatctt
     4261 caccggcttt atccagggcc tgtcccaggg ataacgccgg gtcatcttct gctgacaacc
     4321 ataattgcag ccactgatat tcggcggcag ttatgcgggt atattgcgcc tgaaaatgcg
     4381 gtcgccacac aaggtaataa aactgctcat cgtcagcatt taccttgctg ttgtcttgct
     4441 gctttctcgg gtatttttgc gaatagtagc tgagctgctt gtctatttcg ctaaaggcta
     4501 atggcgtctc ttccgtgttt aatttctgca tatcggtgat gatcgcatct aatggaaatg
     4561 cactactgag tacccgggtg ccggcgctta agctcaggct gagcaggtaa gggtcttgcc
     4621 ccgccaatag attaaacgac tccagatccc ggctggcatc ttcgctgcgc tcactttgat
     4681 gacaggcata atcaagcccg gcaagtgagc caagcagcgc tgcatggtta atggcttgtc
     4741 cggcaataaa ttgcggaaaa tcaaggccga attctcccca gtcatattcg ctttttaacc
     4801 cggtttgcag gtaaccggcg accagctcct gaaaaactgc ttcaccaaca aaagaatgta
     4861 ccgtggcaaa ggtaattttt aatgcgaaac tggcattgtg cagcaagctt ttttgataaa
     4921 cggcaagtcc ctttgccgtt aatggctcac ctgggccagg ttgggaaaaa atcgcttcaa
     4981 gcaaagccga ttgctgatgt tcaagcgtca tggtttgctg cctcttttat tactttatgt
     5041 actgcctgat gatgcgaagt catcggctgt tgtgaatccg gttgataagc gcttgcaaaa
     5101 acttccgcag ctatggcttt tgccttagcc gcttctgcaa ccagcacttg ccacgaaggt
     5161 aactgattat cccattcgat cagcgtggct acggcgccga atctttcaag cgcaaatcgg
     5221 tataaagacc agacttcatc agaaaccgcc tggctatgat cgtctattac cagttctcct
     5281 tcgccggttg gagtgaaacc ggcaaggtgc aattcgccaa cgtagtcttg aggaatggta
     5341 tttagccatt gctgcgtata agcgatcaca tcaagcttgg cgctatcggg ctgcaacagg
     5401 gcatggtttt gggcgttaac cactaagtta ttaatatcaa ttaataactt gcagccggtt
     5461 aagcggcaaa gcctgactaa aaattcgctt tcgctgtagg tggagccggg caatatcagg
     5521 taggccgaca ggttttccac caaaataggg cgctttaata gcgcttgcac ccggctgaca
     5581 ttggccgcca ttatctcaag ggtttcatca ttaaaaggca cgggcaggag gtcgccgccg
     5641 tgaaccgggc ggccgtttac ttctgcccag ctaaaacagg catggtcaga caccatgatc
     5701 ggctggcaaa ttctgatcaa atctgccagc tgctgaattt gtgcctgcgg tgcaggcaaa
     5761 gccgaaccca acccgagaga ggttgcgtgc aggctgatat cgtaatgctg cctgatatcc
     5821 gccagcaggg cgcggcttac accgcctgcg gcaaaaaagt tctccgcatg cagttcaata
     5881 aagtcgatat tagccggagt gtctaaggcg tcctggtaat gggcatgcct taaaccaaca
     5941 ccaactttcg gcaggtttgc cggtgttttt acttgcgtag gaaacataac attttctccg
     6001 gtaatatcga aaacggtact attttgcgct tttcagctgc ttaataattt cacgggcttc
     6061 tttgcgggta tatcccccct ggctcttaca caggccctta gtgactaacc gccattcgcc
     6121 cggatcattg tcaacggttg actgcccggc gcaggaatgg gtgcccgcta aattactgca
     6181 atcgttttgc ccggcggggg atatgccgta gcatttctct tttttcaccc gcttttctgc
     6241 cgcttcggca tcggataacg cgcctaaggc taatgcggcg gcaagggcgg ttgacacggc
     6301 taatttatgg ctcatgtgac tctcctaaag taatactggt ttaatctgct aaaacatctt
     6361 ggtttaaggc taaagctctg cggccggcac ttttctgatg tcctgccagt tttcgtctga
     6421 ctgggataca taaccgggaa catcggttaa ccatttcttt tgtacgtctt tgttgaggtt
     6481 gaggtacagt tttccgtcag cgattttcca ggccaggggg tcggtatcaa atttcttttc
     6541 catggccaca ccgaaagcac agtaaccgcc gtattgcggc gcgtatttgg ccgggttttt
     6601 attgaacagg tcgcggtttt cttcattgct gaagcggtaa atgccgcctt tataggttgc
     6661 ggtgtagtta acattgccca gcttaggcgc cgccatagtg aaataactta cagggtcata
     6721 gcccttaatc gccaggtcgt ttacgtcagc gttcatatcg atgtccgccg caaatgacag
     6781 gctgctggca gctaacagag aaccggctaa aacagatttt aaagtcgtgg taaacataag
     6841 gtttcctcag tggttgtttg ctaatgacgg aggaaataat actgttgaat atcggggccg
     6901 tgggatacaa tcgtccgaac tttgagtcaa atcgtccaga gatgtcatgg atcacctttc
     6961 acaaatcatt gaccacttca agttaaccgc cggggttttc tataccggga aactttgcgg
     7021 cttgtcgcac ttcaatgaaa acgaagccct ggaagggcat attcacctgt tgcgcagcgg
     7081 cgtgctggcg attaacggcc tggacaaatc gccgcttgtg cttgaccagc cttccattgt
     7141 ttttttaccc cggccaacca aacacagcct taaggcgcga gaggtagaca atgccgagtt
     7201 gatttgcgcc aacatcacct acggcagcag cagtaccaac ctgctggcca acgccttgcc
     7261 cgatatgctg attttgccgc tggagtcgtc cccgttttta caaaccaata ttgactggat
     7321 tattgccgag gcgggcggcg accaggaagg acgccaggcg gtaatgaacc gtttgatgga
     7381 gatttttatt atcaatatgc tgcgttcgct actggttaag ggagaacttg cccggggaat
     7441 gctggcgggt ttgatgcacc cgcagctgaa acaggtgttg cagcaactgc ataatgcgcc
     7501 gcaggaaaac tggtcgttaa ataaaatggc cgagctggcg ttgatgtcgc gctcaaaatt
     7561 tgccgaggtt tttaaaagcg tgatcggcca aactcccaat gattacctgc tgagctggcg
     7621 tgtggcaacc gcgcaatcgc tgttaaagca gggcaagagc gttaccctgg tggccaacag
     7681 cgtaggttac gacactgctt cggctttatc ccgggttttc aggaaaacct gcggcatgtc
     7741 gcctaaacaa tggctggata ccttgtaacc ctgttcagtt ctgcaaatac ctggcttaaa
     7801 taaagcctgc ataaaacgcc cgtggctaag cttatagcca cggcaatagt gcctgcccga
     7861 aacacagcat tttaattatg cggggcaggc tatttttctc ttggtctgac tcatttaaat
     7921 actatacttt tcagctgctt aaattcactg tacagcaaag tgtacagtga gcaaaaaact
     7981 atcgtcaaat taaaggctac ggcagccgga tttttaccgg tttttgtact attactatga
     8041 gtggcccttt taaccagtct tgttaatcat aatctgagga ggtgcatgta agagagattt
     8101 ttacaacagg tagctgctga caaacagcca ggcgcttccc ccgagtggta gcacactcag
     8161 aggaagctaa ccatcacaga tataaaaggt atctatcatg gctaaatgta atgatatgcc
     8221 agagtttcac tcgcttaaag ttttattaac aaaactttta acctgccggt taagtaaaac
     8281 cgttcccgcc aaccaatatc catcaaccgc acctgagcca ttatgcccgc ttgcggtatg
     8341 ttcggccgtt tggcaatagg agggtggaga catgagtgaa tatactgaca acccagccga
     8401 caactctgat ttagaatcat cagccccgga agaagaggaa aatcagactg ccgaacacag
     8461 cgacgaacaa aagcccattg ccagtgaact gttagcactg accgatgatt ttgcagagtt
     8521 cagcgctgac tgcgcttttc tctgcgatgc ttttgctgta attgccgagg aacaggaaga
     8581 cataagcgac tacaccagct acggcgtcag gcgctgcagc gactacacca gctacggcgt
     8641 caggcgctgc agcgacagcg tcaaagagca ggtgatagcg cttgaccgga ggatacatgc
     8701 actgcaacgg cgtgtgagtg agcaggatta acgctgttca aagtgtttaa tcttgtaatc
     8761 gcttttgttt gatgttgagc accaaaagcc cggttcctgg agggaggcgg gctttttaag
     8821 ttcaaggggc tgtggtttgt tgatatcagc ataagtgcct ggataggaat aaataagata
     8881 tataaagcag gtgtggttcc tttcggtatt cagttcatcc tggagtgttg ttgctggcgg
     8941 cattatttct gtaaaaaact tccctgattg gcttaatctg aagcaggtgt aaaggctggg
     9001 gtctttgcgt ttttccaatg ttataaatta agttatagcc cggcgtgttt gagggtgatt
     9061 ttgtctatac ttagcctgtc cagtatttaa ggtggtgctg ttaaccgcac cggccaggtt
     9121 aaaggtttta aatcgggcat gaggtttctt gtacaggttt ttataggcat agcgtttggc
     9181 ttgatttcac aagacgccag aacctcgacc tcaccctgtt actcaccatt ccccgaactg
     9241 actgcaacta cgcaaaagcc tgtgctaagt gcaggttacg gcgcatttga cccggcggat
     9301 acttcgcttg ccggtttcag cgaacagagc actattttta ccgacctgaa tgtttttctt
     9361 cccggcagca agtccgggaa tatgcaaaat tattggcagt taagtcatca ttaccgggca
     9421 tttgattttg aagagcctgc gggcactcaa ctgcacagta atggcgatca ccacaagtta
     9481 gcattgcttt ggcacgggct taaccggcaa aagcaacatc aatgggcttt tggcatttct
     9541 ccattgttat cggtaacttc aaaccagcta aaaaataccg gcaggctgaa cagtaatgcc
     9601 tggcagctgc atggttttat tgaataccgg cagttctaca gtaaaaaatg gcaatggcta
     9661 ttgggggctt gtatggatga tcgtttcgat catgttaaag cttatccaat tgcgggagta
     9721 gcaggagata tcaacgaaag aaccaggctt cgcctagcat ttcccgatac taagcttacc
     9781 gttaagttaa cacctcactg gcatgttagt acgttattat cacccgccgg cagcaagtgg
     9841 gatgctttca ataagactgc cgacaaacat tctgactttc attaccgcag ctggcgcttt
     9901 tctgtggctg cccgctggca gtttaacagc cagtggcatt ttaggtttga atgggggcgt
     9961 gagtttgacc ggcgttttag gttcaggcaa ctggctggta atcaagtaag cacggtagct
    10021 gagagtgtta attatgttgc agcgtatatt atctggtatt ggtgataata atatagagca
    10081 gtcacagtat ggcttgattt taggcgacac agcatttgcc gggttatatt tgtcaggccc
    10141 ctttaaatca cctcaatatt aactgcaata aattataggg agacatttcc aagtcgaggg
    10201 caaaaatacg ggaattatta attacggggc aacttttccg ttatcaacgg gggaaaaaat
    10261 tcgtattata tatcttgcta gacttgagct tgaaagtccg tagcccctat catttgattt
    10321 aatttcagct ctctatacgc aattacttgg catcacaaat cgctttacct attgatggca
    10381 cgagcattta ttcctgctgg gcgccgttgc agaatcaatg caggttgatg gttattcaga
    10441 gatcatcaat aaccttgagt ttacgtttcg atgattatca ggaatttaac tgtcgttctg
    10501 ttattgcaga taagacgtgt tctgttacca gttcacttaa tttgtttgac ccattttgtt
    10561 accttaaaat ccatatctgt catcagtgtt agggttgtat ttttgatacc gtagaaagaa
    10621 tcaggcaggc tcagttgatg gcggatatat tgattttcat gcttgtcatt gagctgccct
    10681 gtatatgtta acgggatctt tcattcaaaa ggttatcatc tgtttggagc tgagatgaaa
    10741 tattgtatac aaatgtaagg tctaaagagg tcgggaatga tgtaattgtt tcatttaacc
    10801 tcatcccggg atgaaaataa tgtcgcttgt ttgcattatt gttgttaata acgcctttgt
    10861 caggtaaaac ttttcgcaag atcagttcct cttttatgtt tgcttttatt ggggggcggg
    10921 cagtgctttg ctaaaaggag gttagatgaa gtactgctgc tgttctttat ccgaactgaa
    10981 aaggata
//
