LOCUS       NZ_CP059733            10837 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Thalassomonas viridans strain XOM25 chromosome, complete genome.
ACCESSION   NZ_CP059733
VERSION     NZ_CP059733.1
KEYWORDS    RefSeq.
SOURCE      Thalassomonas viridans
  ORGANISM  Thalassomonas viridans
            Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
            Colwelliaceae; Thalassomonas.
REFERENCE   1  (bases 1 to 6726747)
  AUTHORS   Pheiffer,F., Schneider,Y.K., Hansen,E.H., Andersen,J.H.,
            Isaksson,J., Busche,T., Ruckert,C., Kalinowski,J., Zyl,L.V. and
            Trindade,M.
  TITLE     Bioassay-Guided Fractionation Leads to the Detection of Cholic Acid
            Generated by the Rare Thalassomonas sp
  JOURNAL   Mar Drugs 21 (1), 2 (2022)
   PUBMED   36662175
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726747)
  AUTHORS   Olonade,I., van Zyl,L.J. and Trindade,M.
  TITLE     Draft Genome Sequences of Marine Isolates of Thalassomonas viridans
            and Thalassomonas actiniarum
  JOURNAL   Genome Announc 3 (2), e00297-15 (2015)
   PUBMED   25883295
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6726747)
  AUTHORS   van Zyl,L.J., Busche,T., Ruckert,C., Kalinowski,J. and Trindade,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2020) IMBM, University of the Western Cape, Robert
            Sobukwe, Cape Town, Western Cape 7550, South Africa
REFERENCE   4  (bases 1 to 6726747)
  AUTHORS   Olonade,I., van Zyl,L.J. and Trindade,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2015) IMBM, University of the Western Cape, Robert
            Sobukwe, Cape Town, Western Cape 7550, South Africa
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8; Canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 107.2x; 129.1x
            Sequencing Technology             :: ONT; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000948985.2-RS_2024_03_26
            Annotation Date                   :: 03/26/2024 01:21:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,855
            CDSs (total)                      :: 6,705
            Genes (coding)                    :: 6,644
            CDSs (with protein)               :: 6,644
            Genes (RNA)                       :: 150
            rRNAs                             :: 11, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 117
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 44 of 61
            Pseudo Genes (internal stop)      :: 12 of 61
            Pseudo Genes (multiple problems)  :: 18 of 61
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:09:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4740938
            Orig. end                         :: 4751775
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059733.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10837
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10837
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10837
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5837](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5837
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    132..145
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.86"
                     /tool="antismash"
     misc_feature    complement(266..280)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     gene            complement(471..1709)
                     /locus_tag="SG34_RS20965"
                     /old_locus_tag="SG34_020875"
     CDS             complement(471..1709)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_process="GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011044239.1"
                     /locus_tag="SG34_RS20965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_020875"
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="WP_044842142.1"
                     /transl_table=11
                     /translation="MFQPVSFFIGLRYSRSQNRSGFVSFITFFSIAGILLGVASLITVV
                     SVMNGFEGELKKKILGLVPHIVVSAEQEKPANMPHWQQQREKLLAYSGVKAVTPLIESE
                     ALIQSPSTLQGVLLQGIIPEYETGHIINQHMVAGELSSLDGMPYSLVMGQALAHKLNVS
                     VGDKIRLVIPNKTVFTPMGRVPVQRTFTLTGVFNVGSQIDDAVVYIQSRAAAKLLRRKG
                     DGISQLRLYLNDAFYAGELTPQLKQDLSGYTFTTWNESQGALFAAVSMEKNMMWLMLSL
                     IVAVAAFNIVSALVMVVIDKQGEIGILQTLGLARGEIVKIFITQGMVNGLWGVVLGSIL
                     GVLLTLNLNTLMSVTGINIFGPGYASQLLPIQLEAWNVAVIVFSALMMSFVATLYPAYR
                     ASKTQPAEVLRNE"
     gene            complement(1853..2980)
                     /gene="mnmH"
                     /locus_tag="SG34_RS20970"
                     /old_locus_tag="SG34_020880"
     CDS             complement(1853..2980)
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="mnmH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011041618.1"
                     /locus_tag="SG34_RS20970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_020880"
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="WP_044842143.1"
                     /transl_table=11
                     /translation="MSLDVTAKVNQAPEEARPDTCDYHRIFASDLPLLDLRAPVEFARG
                     AFEQAVNIPLMTDDERAAVGTCYKEQGQQAAITLGHQLVSGKTRAGREQAWQEFIGQHP
                     QGYLYCFRGGLRSRTVQAFLKEAGVHYPLVKGGYKALRQYLLDQLIQLSSHPLVILGGK
                     TGCNKTDFIASRSDSLDLEGAAGHRGSSFGGFAWPQSTQITFENKLAQQYIRRNFAPGQ
                     RILLEDEGRVIGSVHVPHELREKMLLSPVVVVEESFEFRLEQLFNEYIVKMVQDFVALK
                     GEEPGRQAFSEYFHQGVFKVRKRLGMQRYQALLALLEKATSGLHRGELNGYLDVLRELM
                     VQYYDPMYDYQLGLKKERIVFRGNSAECRTYLDAL"
     gene            complement(2980..4011)
                     /gene="selD"
                     /locus_tag="SG34_RS20975"
                     /old_locus_tag="SG34_020885"
     CDS             complement(2980..4011)
                     /EC_number="2.7.9.3"
                     /GO_function="GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="selD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019026547.1"
                     /locus_tag="SG34_RS20975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_020885"
                     /product="selenide, water dikinase SelD"
                     /protein_id="WP_044842150.1"
                     /transl_table=11
                     /translation="MSQVKLTQYSHGAGCGCKIAPAVLDDILKSSLSLPQQPALLVGNQ
                     SKDDAAVYDIGNNQAVISTTDFFMPIVDDPTDFGKIAACNAISDIYAMGGKPLMAIAIL
                     GWPINKLAPEVAQKVLDGGRMICAQAGIPLAGGHSIDAPEPIFGLAVTGLIDTDKVKRN
                     NNAGAGDLLYLTKPLGVGILSTAQKQGKLQPEHADIAPDVMKTLNSPGLAFAELEGVSA
                     MTDVTGFALLGHLLEMCQGSGLAAELDFAKVPLLEQVENYIDLGCIPGGCGRNFQSFGH
                     NVSELTPLQQTILCDPQTSGGLLVAVKPAAREEFESLAQKLGLLLEPIGKMVAEQSPVV
                     SVV"
     misc_feature    complement(4254..4269)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.5"
                     /tool="antismash"
     gene            4451..4918
                     /locus_tag="SG34_RS20980"
                     /old_locus_tag="SG34_020890"
     CDS             4451..4918
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006082774.1"
                     /locus_tag="SG34_RS20980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_020890"
                     /product="hypothetical protein"
                     /protein_id="WP_044842144.1"
                     /transl_table=11
                     /translation="MKANTKAKLQGTAVALAVAGMMGCAQTSSQGGSSSYSASNSAELG
                     HCYGVNKCSGHNDCKTAQNACKGQASCKGHGFVAMPTKSCGDIGGTVKDDWRGKVTTAD
                     MAHCYNVNVCGGHNDCKTANNACKGQASCKGQGFVKMSAKACNDVGGKVGK"
     misc_feature    4481..4494
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.82"
                     /tool="antismash"
     gene            5001..5837
                     /locus_tag="SG34_RS20985"
                     /old_locus_tag="SG34_020895"
     CDS             5001..5837
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010369803.1"
                     /locus_tag="SG34_RS20985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_020895"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_044842151.1"
                     /sec_met_domain="DUF692 (E-value: 5.8e-100, bitscore:
                     332.3, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGFGLGLRTDHFQDVLEQKPALDWFEVVSENFMVDGGKPKHYLHA
                     IREHYPMVMHGVSMSIGSTEPLNMDYLRKLKVLANELQPEWLSDHLCWSTNKQVNSHDL
                     LPLPYNEEALQHVADRISRVQDFLGRQILIENVSSYMTYKDSDMTEWQFINEVVKRADC
                     FMLLDINNIYVSARNHNFAPLDYLNGIDTDRVRQFHLAGHTDYGDYVIDTHDHEVADPV
                     WELYRRALQRFGWVSTMIERDDNIPPFAELMKELEIARNIATQTLNRQTFGGKQKL"
     gene            5840..6625
                     /locus_tag="SG34_RS20990"
                     /old_locus_tag="SG34_020900"
     CDS             5840..6625
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /locus_tag="SG34_RS20990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_020900"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_053047462.1"
                     /transl_table=11
                     /translation="MEKTSLEKLQQQMIDYLLCDDEAICNKIVSQGAIDNHTRLDIYKN
                     AYRMRLKETLDTDHDILGLYLGDELFEQMVNGYIDLYPSRYTSLRQYADNLPEFLATQP
                     PFADFPLLSELARFERLLLTAFDAADVTRFTLEDIQDIAHEQWPGLVFHFHPSVQLAHM
                     HWNSVESWQALKTGQAPDPAREHKSCWLLWRNRDRLTEFRSISEEEASLFHMILAGENF
                     SALCEFLLQNHDGSENDNVTTVALNYLSGWIEQGLLRKV"
     gene            6736..6903
                     /locus_tag="SG34_RS20995"
                     /old_locus_tag="SG34_020905"
     CDS             6736..6903
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019025973.1"
                     /locus_tag="SG34_RS20995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_020905"
                     /product="DUF2897 family protein"
                     /protein_id="WP_084724162.1"
                     /transl_table=11
                     /translation="MSLTAVIIILLCLGVIVSGILLLKQSAKKFNLTEQQLKEIKARNR
                     QLEKEEQQEK"
     misc_feature    6960..6973
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 19.76"
                     /tool="antismash"
     gene            6987..7577
                     /gene="can"
                     /locus_tag="SG34_RS21000"
                     /old_locus_tag="SG34_020910"
     CDS             6987..7577
                     /GO_function="GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="can"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017222912.1"
                     /locus_tag="SG34_RS21000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_020910"
                     /product="carbonate dehydratase"
                     /protein_id="WP_044842145.1"
                     /transl_table=11
                     /translation="MSSIDHLFDNNKKWAQEIKQQQPDFFQQLSQQQTPDYLWIGCSDS
                     RVPANELLGMKPGEVFVHRNIANQVIHTDLNCLSVIQYAVEVLKVKHIIVCGHYGCGGV
                     HASADNNSYGLIDNWLRHIQDVYRFHKEQLDAETDHGKRMNLLCELNVIEQVNNVCNTT
                     ILRNAREAGQNITVHGFVYNLADGILKDLKVSV"
     misc_feature    complement(7010..7023)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 20.19"
                     /tool="antismash"
     gene            complement(7662..7997)
                     /locus_tag="SG34_RS21005"
                     /old_locus_tag="SG34_020915"
     CDS             complement(7662..7997)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SG34_RS21005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SG34_020915"
                     /product="hypothetical protein"
                     /protein_id="WP_274038358.1"
                     /transl_table=11
                     /translation="MSESTSTPQNEAARRKAQLSALVDLTDDFSQFHQECAFLCDAFAA
                     VAQEPECISEETSEGIRHMSYWLKGQAKEYYQRIDDLYQEAYSHNKQAVVQEKAQESHE
                     NREDEQH"
     misc_feature    8284..8299
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.27"
                     /tool="antismash"
     misc_feature    8400..8418
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 25.74"
                     /tool="antismash"
     misc_feature    8430..8443
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.31"
                     /tool="antismash"
     gene            complement(8650..9693)
                     /gene="hppD"
                     /locus_tag="SG34_RS21010"
                     /old_locus_tag="SG34_020920"
     CDS             complement(8650..9693)
                     /EC_number="1.13.11.27"
                     /GO_function="GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA]"
                     /GO_process="GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hppD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016899429.1"
                     /locus_tag="SG34_RS21010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_020920"
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="WP_044839716.1"
                     /transl_table=11
                     /translation="MTEVNNPLNLRGIEFTEYATPDSDFMENAFYGFGFSKTKKFKGRD
                     IDYFNQNDIHFLLNNEQAGFSREFAKSHGPAICSMGWRVDDAEFAFSEAIKRGAKSAQD
                     APNKMPYPAIYGIGDSLIYFVDKFGDKGSIYEDDFEALENPVEVEEKGFKAVDHLTNNV
                     YQGTMEHWANFYKDVFGFTEVRYFDIKGIQTALVSYALQSPCGTFCIPINEGKGDKNNQ
                     IDEYLDEYNGPGVQHLAFITDDLVSSLDKIDRSIIDTLDIVPEYYDDIFDRVPWVKEDK
                     KRIQEHQILVDSQKENSYLLQIFTKNIFGPIFIEMIQRVDDRGFGEGNFTALFKSIELN
                     QMERGVL"
     misc_feature    complement(10103..10116)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.28"
                     /tool="antismash"
ORIGIN
        1 ccagcgcccg ggcaatagcc acccgctggc gctccccccc ggaaagctct cccggacggt
       61 gttcacacct gtgacccaaa ccgactttgg tcagcatctc tttcgcctta acccgggcgc
      121 ttttggcatt gtcgccgcgg atcaacaaag gcatggcgac attttccaat gcggaaaact
      181 ccatcatcaa atggtggaac tggtaaataa aaccgatatg gcggttgcga aaagccgccc
      241 tttccttatc cgataattta tggatattga catcattgat aaacacctcg ccggcactgg
      301 gcacatccag cgcaccggcc agatgcaaga aggtactttt accacatccc gagctgccga
      361 caaccgccaa tagctcgcct tgctcaacct caaggtccag gccctgcaag accttagttt
      421 ctatctggcc ttgctggtaa gattttttta actggcggca aactaacacc ttattcattt
      481 cgtaaaacct ctgccggttg cgttttagat gcccggtagg cgggatacag ggtcgcaaca
      541 aaactcatca ttaacgcgga aaatacaatc accgccacat tccaggcttc aagctgtatc
      601 ggtaacaact gtgacgcata acccggacca aaaatattaa taccggttac cgacatcaag
      661 gtattgagat tcagggtaag caatacccct aaaatgctgc ccaataccac gccccataaa
      721 ccgttaacca tgccctgggt gataaaaatc ttgacgattt cccccctggc caggcccagg
      781 gtctgtaaaa tgccgatttc cccctgcttg tcgatcacca ccatcaccag cgccgagacg
      841 atattaaacg cggctaccgc aacaatcagg ctcagcatca gccacatcat gtttttttcc
      901 atgctgacgg cggcaaagag cgccccctga ctttcattcc aggtggtaaa ggtataaccc
      961 gacaaatcct gcttcagctg cggcgtaagc tcaccggcat aaaaagcatc gttaaggtac
     1021 agcctcaact ggctaatgcc atcccccttg cggcgcagta acttggcggc ggcccggctc
     1081 tggatataaa ccacggcatc gtcaatctgc gagccgacat taaacacccc ggtcagggtg
     1141 aaggttcttt gcaccggcac acgccccata ggggtaaaca cggttttatt agggatcacc
     1201 aggcgaattt tatcgccgac actgacattt aatttatgcg ccagcgcctg ccccataacc
     1261 agggaataag gcatgccgtc caggctggat aattcacccg ccaccatatg ctggttaata
     1321 atatgtccgg tctcatattc aggaatgatg ccctgcagca ataccccttg cagggtcgaa
     1381 ggcgactgga tcagcgcctc gctttcgatc agcggcgtaa ccgccttcac cccggagtaa
     1441 gccagcaatt tttccctttg ctgctgccag tgtggcatat tcgccggttt ctcctgttcg
     1501 gcagacacca caatatgggg caccaggccg agaatttttt tcttcagctc cccttcaaag
     1561 ccattcatca cagacacaac cgtgatcaac gaggcaaccc ccagcaaaat cccggcaatg
     1621 gagaaaaagg taataaagga gacgaacccg gaacggttct ggctacggct gtaacgcaag
     1681 ccaataaaaa agctaaccgg ttgaaacatg caattgatac actaagtgtt tgattattat
     1741 attatatgac aataaagcta atagatatca cagatatcga aaatatcatc gtcctgctca
     1801 ggcgccttag cataccacaa aggcgcctgg tttaagtcat taaaaaagca cattaaagtg
     1861 catctaaata tgtccggcac tcggcgctgt tgccgcgaaa gacgatacgc tcctttttca
     1921 aacccaattg gtaatcgtac atgggatcat aatactgcac catcagctcc ctgagcacgt
     1981 ccaaatagcc gtttaactca ccccggtgca gtcccgaagt ggctttttcc agcaaagcga
     2041 gcagggcctg gtatctttgc atacccaggc gtttacgcac cttaaacact ccctgatgga
     2101 aatactcgct aaaggcctgc ctgcccggct cttcgccttt cagggcaaca aagtcctgca
     2161 ccattttcac tatatactca ttaaagagct gttccaggcg aaactcgaag ctttcttcca
     2221 ccaccaccac gggcgagagc aacatttttt ccctgagctc atgcggcaca tggacactgc
     2281 cgatcacacg gccttcatct tccagcagga tgcgctgccc gggagcaaaa ttccggcgga
     2341 tatattgctg cgccagttta ttttcaaaag tgatctgggt cgactggggc caggcaaaac
     2401 cgccgaaact ggatcccctg tggccggccg ccccttccag atccaggcta tcgctacggc
     2461 tggcgataaa gtcggtttta ttacagccgg ttttcccgcc gaggatcact aaaggatgac
     2521 tgcttaactg aataagctga tccagcaaat actgccttaa ggccttataa ccccctttca
     2581 ccaaaggata gtgaacaccc gcttccttta aaaacgcctg cacagtgcgc gagcgcaaac
     2641 cgccgcgaaa acaatacaga taaccctggg gatgctggcc gataaactcc tgccaggcct
     2701 gctcccgccc ggcccgggtt tttcccgata ccagctggtg cccaagggtt atcgctgcct
     2761 gctggccctg ctccttgtag caggtgccga cagcagccct ttcatcgtcc gtcatcaggg
     2821 gaatattcac cgcctgttca aaagcgccgc gggcaaactc cacaggagcg cgtaaatcca
     2881 gtaagggcaa gtcagaagca aatatcctgt ggtaatcaca ggtatcgggg cgagcctctt
     2941 ccggggcttg atttaccttg gctgttacat ctaagctcat taaacaacac tcaccactgg
     3001 actttgctcc gctaccattt tcccgatagg ctcaagcaac aaacccagct tttgcgccag
     3061 ggattcaaat tcttcccggg cagcaggttt taccgccacc agcaaaccgc cgctggtttg
     3121 cgggtcgcac aaaatggttt gctgcagagg cgtcagctca ctgacgttat ggccgaaact
     3181 ttggaaattg cggccgcagc ctcccgggat acagcccagg tcgatatagt tttccacctg
     3241 ctccagcaaa ggcactttgg caaagtccag ctcggcggcc agtccgctgc cctggcacat
     3301 ttccagcaga tgtcccaata aggcaaagcc tgtcacatcc gtcatggcgc ttacgccttc
     3361 caattcagca aaagccagtc ccggactgtt aagggttttc atcacatccg gggcaatatc
     3421 ggcatgctcg ggctgcaatt tgccctgttt ttgcgccgtg cttaaaatgc cgacacctaa
     3481 aggtttggtc agatatagca gatcaccggc cccggcattg ttgttgcgct taaccttgtc
     3541 ggtatcgatc agcccggtca ccgccaggcc gaaaatcggc tcaggtgcat cgatagaatg
     3601 acctcctgcc agcgggatcc cggcctgtgc gcagatcata cgtccgccgt ccagtacctt
     3661 ttgcgccacc tcgggcgcca gcttattgat gggccagccc aaaatcgcaa tcgccatcaa
     3721 aggcttgccc cccatggcat aaatatcact gatggcatta caagcggcta tcttgccgaa
     3781 atccgtagga tcatcaacta tcggcataaa aaaatctgtg gtggaaatca ccgcctggtt
     3841 attgccgata tcatacacgg cagcatcgtc tttactctgg ttgcccacca gcagagccgg
     3901 ctgctgtggc agggacaagg aagacttgag gatatcatct aaaaccgccg gggcaatttt
     3961 acagccgcaa ccggcgccat gggagtactg ggttaatttt acttggctca cgttcaatcc
     4021 tttattaata aaaaagagct gttgaaacaa ctgctgaaaa tgctttagaa gcagatttac
     4081 cggcctaatg tacccgaatc ctataagaga tccagtaaaa caggcccctg ttcaccaaaa
     4141 gaaaaggcaa gggcgtgact ttcacggtta acaactgaca gttaaccggc tgaagtggca
     4201 gacatttaat cgccaaaaga atatttacgt ctgtttttca aacagtaacg acagtataaa
     4261 aaatgtcaac tatagtaaat ctggcatcca ggaaaataaa aaacccgcag taaagtgaag
     4321 gaaacgttca gttttgtgac caaattgtga taactcggcc tttgtttttc tctaacatgc
     4381 gttatcacag gcagtgctca aaagaataag cagtaaagca ttaactaatt gactaaaacc
     4441 ggagaatcac atgaaagcaa acactaaagc aaagttacaa ggcacagcgg ttgccctggc
     4501 cgttgccggc atgatgggct gcgcgcaaac ctccagccag ggcggctctt cctcatattc
     4561 tgccagcaac agcgcagaat taggccattg ctacggcgta aataaatgct cgggccataa
     4621 tgactgcaaa accgcccaaa acgcctgtaa aggccaggca tcctgtaaag gtcacggttt
     4681 tgtcgccatg cccaccaagt cctgcggcga tatcggcggc acggtaaaag atgactggcg
     4741 cggcaaagta acgaccgcag acatggccca ctgctacaac gtcaatgttt gcggcggcca
     4801 caacgactgt aaaaccgcca acaacgcctg taagggccag gcatcctgta aaggccaagg
     4861 attcgtgaaa atgtcagcca aagcctgtaa tgatgtaggt ggcaaagttg gcaaataagg
     4921 caacagcagc aagcgggtta acccccgctt cttcacccca aaagccgcag gaatcccggc
     4981 aagccacccc ggaacatttc ctgggttttg gcctgggact acgcacggat catttccagg
     5041 atgtcctgga acaaaaaccc gcccttgact ggtttgaggt ggtctcggaa aactttatgg
     5101 tggacggcgg caaacccaag cattatctgc acgccatacg cgaacattat cccatggtga
     5161 tgcacggggt ttccatgtct atcggctcca ccgaaccgct gaatatggac tatttgcgca
     5221 aacttaaagt actggccaat gaactacaac ccgagtggct gtcggaccac ctgtgctgga
     5281 gcacaaacaa gcaagtcaac agccatgacc tgctgccttt gccctataac gaagaggcgc
     5341 tgcagcatgt ggcggatcgc atctcccggg tgcaggactt tctcggccgg cagatcctga
     5401 tagaaaatgt ctccagctat atgacctata aagactcgga catgaccgaa tggcagttta
     5461 tcaacgaggt ggtcaaacgc gccgattgct ttatgttgct cgatatcaac aacatctatg
     5521 tcagcgcccg caaccataac tttgccccat tggattacct caacggcata gacaccgaca
     5581 gggtcaggca attccacctg gcaggacaca cggattacgg cgattatgtg atcgacaccc
     5641 atgaccacga ggtggccgat cctgtctggg agctgtaccg ccgggcatta cagcgttttg
     5701 gctgggtcag caccatgata gagcgcgatg acaatatccc cccttttgcc gagctcatga
     5761 aagagctgga aattgcccgc aatattgcca cgcaaaccct taaccgccag acattcggcg
     5821 gcaagcaaaa gttataacca tggaaaaaac ttctttagaa aaactgcagc agcagatgat
     5881 agattatttg ctctgcgacg atgaggccat ttgcaataaa attgtcagcc agggggccat
     5941 agataaccat acccggctcg atatctataa aaatgcctac cggatgcgcc tcaaggaaac
     6001 cctggatacc gaccacgata ttctcggcct gtaccttggt gacgaactgt tcgaacagat
     6061 ggttaacggt tatatcgacc tctatccttc ccgctatacc agtttgcgcc agtatgccga
     6121 taatttaccg gaatttttgg cgacacaacc cccctttgcc gatttccctc tgctgagcga
     6181 acttgcccgt ttcgagcgtt tattactgac ggcattcgac gccgccgatg tcacccggtt
     6241 taccctggaa gacatccagg acattgccca tgaacaatgg ccgggcctgg tgtttcattt
     6301 tcatcccagt gtccagcttg cccatatgca ctggaacagc gtcgaaagct ggcaggcatt
     6361 aaaaaccgga caggcccctg accccgccag agagcataaa agctgctggc tgctgtggcg
     6421 caaccgggac aggcttacgg aatttcgctc gatatcggaa gaagaagcca gtttatttca
     6481 tatgatactg gcaggagaaa acttcagcgc cttgtgtgaa tttttactgc aaaaccatga
     6541 cggcagcgaa aatgacaatg tcaccactgt ggccctgaac tatttatccg gctggattga
     6601 acagggctta cttcgcaagg tttagttcgc aaggtatagc ccgcaagaca tagttcgcaa
     6661 gacataagca gacaaactat gaccgacgcg caattgatca tataatggcg gcaaaagaaa
     6721 tcatccggag ttatcatgtc cctgaccgct gtgatcatta tactgctttg tcttggggtt
     6781 attgtcagcg gcattttgct gctgaagcaa tctgcaaaaa agttcaacct caccgaacag
     6841 cagttaaaag aaatcaaggc ccgcaaccgc cagctggaaa aagaagagca acaagaaaaa
     6901 taacaggttt gaccaagttt tgctttactt aaacaagcca tgacataaaa tgtcatgaga
     6961 tttttaatta aagtaaaagg ctttttatgt cgtccataga ccatttgttt gataataata
     7021 aaaagtgggc ccaggagata aaacagcaac aacctgattt tttccagcaa ttgtcccagc
     7081 aacaaacccc agattattta tggatcggct gttccgattc ccgggtgccc gccaatgaat
     7141 tattgggcat gaaaccggga gaggttttcg tccaccgtaa tatcgccaac caggtaatac
     7201 atacagacct caactgtctt tccgtgatcc agtacgcggt tgaagtgctt aaagtgaaac
     7261 atattatcgt gtgcggccat tacggctgcg gtggggttca tgcttctgcc gacaacaaca
     7321 gttacggttt gatcgataac tggctcaggc atattcagga cgtttaccgt ttccacaaag
     7381 aacagctcga cgcagaaacg gaccacggca aacgcatgaa cctgttgtgc gaactaaatg
     7441 taatcgagca ggtaaacaat gtctgcaata ccactatttt gcgcaatgcc cgcgaggcag
     7501 gccagaacat cacggttcat ggttttgttt ataaccttgc cgacggcatt ttaaaagacc
     7561 tcaaagtgtc ggtataaggg cttcccttcc ccggcaatct tcccggtcaa agataaacag
     7621 gccctgatgc cagccagtta aaactgactg gcattcaagt gctagtgttg ttcatcctcc
     7681 cggttttcat gactttcttg tgctttttcc tgtactacag cctgtttatt gtggctgtag
     7741 gcttcctggt agagatcgtc aattctctgg taatactcct ttgcctggcc tttaagccaa
     7801 tagctcatat ggcgtatgcc ttcgctggtt tcctcgctga tgcactcagg ctcctgggct
     7861 acggcggcga aagcgtcgca gagaaaggcg cattcctggt ggaactgaga aaagtcgtcg
     7921 gtcaggtcga caagcgccga cagctgggct ttccttctgg cggcttcatt ttgcggagtt
     7981 gaggtggatt cagacatgag cttgccctcc tgcaaaggtt aacggcacac ttctattgat
     8041 aaaacagcta tcgataaaac agctggcgct ggaacagcca ataaaaccgt ggagtgaatt
     8101 tataccggca ttactggtat tagcaaagct gtgccggcgg cggggtagta tattttttgt
     8161 tgcaaaaact ttaaccgagt gcaactctgg catatcatta attttagcca taatcgatac
     8221 ctcttacgta tcagttttgg ttagctatct tcgggtgttc gagcacctga ggatagcgcc
     8281 ttgtttacca tttttttacc ctcttgttta tcaggtaatc ggccggtaac tgaccggtga
     8341 atcatctgtg tttgaagggc ctcctaagtg tggggtgact attaaggtat agtataagtg
     8401 gctggatata tcaacagcgt ggagggggta ttttaagaaa taattacttc ttattaagct
     8461 gttgataatt agttattttt atccttgtcg gcgggcggtt tatcgaataa aaagaatggc
     8521 ggctcggaca gattcgataa caggaaaaaa tcaccgggcc attgaagctt tatcttaaag
     8581 acataaaaaa atccgaagtc ccattaagac ttcggatttt tatctacgta ggggaccgca
     8641 caaacgattt tacagtacgc cgcgttccat ctggttcagt tcgatagatt tgaacagggc
     8701 ggtaaagtta ccttcaccaa agccgcggtc gtcaacacgc tggatcattt cgataaagat
     8761 aggaccgaaa atgttcttag tgaaaatctg cagcaggtag ctgttttctt tctggctgtc
     8821 taccaggatc tggtgttcct ggatacgttt cttgtcttct ttcacccaag gtacgcggtc
     8881 gaagatatca tcataatact ccggcactat gtccagggta tcgataatag aacggtcgat
     8941 cttatccagt gagctcacca ggtcgtcggt gataaacgcc aggtgctgta cgcccgggcc
     9001 gttgtactcg tccaggtact catcgatctg gttgttctta tcgcctttac cttcgttgat
     9061 cggaatgcag aaagtgccgc aaggcgactg cagtgcataa gaaaccagcg ccgtctggat
     9121 acctttgata tcaaagtagc gcacttcggt aaaaccgaaa acatctttat agaagtttgc
     9181 ccagtgctcc atagtgccct ggtaaacatt gttggtcagg tgatcaaccg ctttaaagcc
     9241 tttttcttct acctctaccg ggttttccag ggcctcaaag tcgtcctcat aaatagaacc
     9301 tttatcgccg aatttatcaa cgaaataaat caggctgtcc cctatgccgt aaatggccgg
     9361 gtatggcatt ttgttcggtg catcttgtgc agatttggcg ccgcgtttga ttgcttcact
     9421 aaaagcgaat tcggcatcgt ctacccgcca acccatagaa caaatggccg gaccgtggct
     9481 cttggcaaac tcacgggaga aacccgcctg ctcgttgttt aataagaagt gaatatcatt
     9541 ctggttgaag taatcgatat cacgcccttt aaacttttta gtttttgaaa agccaaagcc
     9601 atagaaagca ttttccatga agtcgctgtc aggtgttgcg tattcggtaa attcaatgcc
     9661 gcgtaaatta agcgggttgt ttacttcggt catattggta tctctcttca atgttataac
     9721 ttcattatta atctgaattc ggcaaagaaa aagacctgca ggaaacagaa aaaaagcggg
     9781 gctgtaacag aaaatgtacg cacaaaacga caaaaacctg atctacatca agccaacgca
     9841 gataagccct gcaaggcagc cgtaaagaca agtttacacc ggtattttcc tagtaaaaaa
     9901 tcgcttttta ccggcatttc ccggtgggaa aaacaggccg ataaaagccc tgtccggagg
     9961 ggaaaatcac aatttaacag agaagaaagc agcggattaa tacagcagca attaaattga
    10021 cggcaataac ccctgctctt attcatccat gaagccgcag gataaatact tccgtgtata
    10081 aaaaaagcgc tgaaaagcgc cttttttatt aaacattttg taaggccgtc attcccggcc
    10141 aaacatcttt agtttgtgcg tcttggacga tcggcacggt cgttataacg acgcggacgt
    10201 tcgtgcatgg ccggtgcgtc ggaaacggaa atctccaatg cctggcggcg tacccgtacc
    10261 gttttcagct gctggaaagc accggtagga atgccttttg gcaactgcac ataactgtgg
    10321 cgctcgtgca ggttaatggc gcctatatag ctgctgtcca gggaaatctc attggcaatc
    10381 gcgccgacga tatcacccgg cttggcgccg tgctctttac ctacctctaa acggtaagtc
    10441 tgccagtcga cgtcgctacg taccgcttta ggcttacgcg gcgcacggtt gtcgcggtaa
    10501 ccgtcacggc cgccgcgcat atcgcggttg ccgcggttat cgcgtccgtc acggcgacgg
    10561 tcattgcggt caaaacggtc gcggctttca cggcgcggtt taggatcttc tttcggctgc
    10621 agcggctgct tcagctgctt ctgatacagc agggctgcgg ccagatctac catggaaagc
    10681 tctgattcgt tggccatgct ctcgatgatt tcacgcatgc ccgccagatc tttctcgcca
    10741 acaaccgaaa ccagctcttc acgggtacgt tcgatacggg ctttaccgat ttcctgaatg
    10801 gtcggcagct cataagcttc tatggtacct gaagtta
//
