LOCUS       NZ_CP059733            12187 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Thalassomonas viridans strain XOM25 chromosome, complete genome.
ACCESSION   NZ_CP059733
VERSION     NZ_CP059733.1
KEYWORDS    RefSeq.
SOURCE      Thalassomonas viridans
  ORGANISM  Thalassomonas viridans
            Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
            Colwelliaceae; Thalassomonas.
REFERENCE   1  (bases 1 to 6726747)
  AUTHORS   Pheiffer,F., Schneider,Y.K., Hansen,E.H., Andersen,J.H.,
            Isaksson,J., Busche,T., Ruckert,C., Kalinowski,J., Zyl,L.V. and
            Trindade,M.
  TITLE     Bioassay-Guided Fractionation Leads to the Detection of Cholic Acid
            Generated by the Rare Thalassomonas sp
  JOURNAL   Mar Drugs 21 (1), 2 (2022)
   PUBMED   36662175
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726747)
  AUTHORS   Olonade,I., van Zyl,L.J. and Trindade,M.
  TITLE     Draft Genome Sequences of Marine Isolates of Thalassomonas viridans
            and Thalassomonas actiniarum
  JOURNAL   Genome Announc 3 (2), e00297-15 (2015)
   PUBMED   25883295
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6726747)
  AUTHORS   van Zyl,L.J., Busche,T., Ruckert,C., Kalinowski,J. and Trindade,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2020) IMBM, University of the Western Cape, Robert
            Sobukwe, Cape Town, Western Cape 7550, South Africa
REFERENCE   4  (bases 1 to 6726747)
  AUTHORS   Olonade,I., van Zyl,L.J. and Trindade,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2015) IMBM, University of the Western Cape, Robert
            Sobukwe, Cape Town, Western Cape 7550, South Africa
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8; Canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 107.2x; 129.1x
            Sequencing Technology             :: ONT; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000948985.2-RS_2024_03_26
            Annotation Date                   :: 03/26/2024 01:21:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,855
            CDSs (total)                      :: 6,705
            Genes (coding)                    :: 6,644
            CDSs (with protein)               :: 6,644
            Genes (RNA)                       :: 150
            rRNAs                             :: 11, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 117
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 44 of 61
            Pseudo Genes (internal stop)      :: 12 of 61
            Pseudo Genes (multiple problems)  :: 18 of 61
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:09:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3361786
            Orig. end                         :: 3373973
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059733.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12187
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12187
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12187
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7187](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7187)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    811..827
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: strong, score: 24.74"
                     /tool="antismash"
     gene            complement(1120..1527)
                     /locus_tag="SG34_RS15030"
                     /old_locus_tag="SG34_014965"
     CDS             complement(1120..1527)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014976937.1"
                     /locus_tag="SG34_RS15030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_014965"
                     /product="GFA family protein"
                     /protein_id="WP_044840113.1"
                     /transl_table=11
                     /translation="MSAIKTGSCLCGKVSFEITGEFDNFFLCHCQYCQKDTGSAHAASL
                     FSTSAKLNWLSGQDNIQTFNLPQTRHVKSFCLTCGSAMPNVQMDGQLLVVPAGSLDVKV
                     SNKPDAHIFVASKANWDDNLENVARMETFPG"
     gene            complement(1691..1954)
                     /locus_tag="SG34_RS15035"
                     /old_locus_tag="SG34_014970"
     CDS             complement(1691..1954)
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /GO_process="GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF020421.4"
                     /locus_tag="SG34_RS15035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_014970"
                     /product="SymE family type I addiction module toxin"
                     /protein_id="WP_044840112.1"
                     /transl_table=11
                     /translation="MADCHHTPELASAKVKYPTYRRLTVLETTCEAPSKAHCIGINYVP
                     VDLEPCIVLRGKWLRKTGFTPGQNVLITINPEQLMITPKHDE"
     misc_feature    2132..2149
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.23"
                     /tool="antismash"
     gene            2696..4219
                     /gene="ltrA"
                     /locus_tag="SG34_RS15040"
                     /old_locus_tag="SG34_014975"
     CDS             2696..4219
                     /EC_number="2.7.7.49"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ltrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008109360.1"
                     /locus_tag="SG34_RS15040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_014975"
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_044838251.1"
                     /transl_table=11
                     /translation="MIISELQRKLATWTKTDKTRRVNRLLRLISHPQWLCKAAEVTLSS
                     KGAKTPGVDGMTKIHFQAKLDDYLTEIRSDLLSGDYQPMPARRIYIPKANKKQRPLGIP
                     TLRDRIVQRGMLMAMEPIWENDFHSLSYGFRPERSVHHAIRTVKLQLTDTSDSKGRWVI
                     EGDLSSYFDTVHHRLLMKGVRKRINCQRFNELLWRFIKAGHIERNLFCATSEGVPQGGV
                     ISPLLSNIMLNEFDQYLDKCYLSKKARKDRWNWNHSIKSKRKVAIDENRQWKPAVAYCR
                     YADDFLVIVKGNKQQAEVIRDQCRDFLEGKLKLTLNMDKTHITHVNDGFIFLGHRIIRK
                     RGPKGNMRVVSGIPKDKAKAFSYSLSKALSGDYSCSKIDKVEQLSRKLKGWAQFYRHTD
                     YTSQVYRKIDRIVFWKLAKWLARKYRCSIKSLMIKWIKRPTPSQAKTWVLFGKSNRGNL
                     CGASLFRLVSSPKLQFRWRLPEYNPYLRDEMRNTVTSRYSDVAMAVSHN"
     gene            complement(4524..4925)
                     /locus_tag="SG34_RS15045"
                     /old_locus_tag="SG34_014980"
     CDS             complement(4524..4925)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010558463.1"
                     /locus_tag="SG34_RS15045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_014980"
                     /product="GFA family protein"
                     /protein_id="WP_044842716.1"
                     /transl_table=11
                     /translation="MTKGNCNCGAVTFEITTEISDVYICHCSICRKSTGSGGIAVLVIA
                     NDDFHWLTGTKLINTWHKPGHDWQTSFCKNCGSPLPGANDDARMHVPAGLISEGGKNLK
                     VAHHLWVDSKAPWEEIGTSGKKHPESFQG"
     gene            complement(5001..7187)
                     /locus_tag="SG34_RS15050"
                     /old_locus_tag="SG34_014985"
     CDS             complement(5001..7187)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019027627.1"
                     /locus_tag="SG34_RS15050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_014985"
                     /product="OsmC domain/YcaO domain-containing protein"
                     /protein_id="WP_274038285.1"
                     /sec_met_domain="YcaO (E-value: 4.5e-72, bitscore: 241.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIKVNYLDNLRIEAKFDDFSVIADQPIRYKGDGSAPSPFDYFLA
                     SSALCAAYFVKVYCAARDIPTDNIRLSQNNIVDPEDRYNQIFQIQVELPESISEKDRQG
                     ILRSIDRCTVKKVVQTGPEFKVEAVESLEEDAQAMLMAQPEGDASTYILGKDLPLEETI
                     ANMTGILAKLGMKIEISSWRNIVPNVWSLHIRDAASPMCFTNGKGATKESALCSALGEF
                     IERLNCNFFYNDQYFGPEIANSDFVHYPHEEWFKPGPNDELPAGILDDYSLGIYDPDKE
                     LRGSHLIDTNSGNVERGICSIPYVRHSDGETVFFPSNLIENLFLSNGMSAGNNQHEAQV
                     QCLSEIFERAVKRQIIEQEIVLPDVPMAVLEKYPGILAGIQGLEAQGFPVVVKDASLGG
                     QFPVMCVTLMNPRTGGVFASFGAHPSFEVALERSLTELLQGRSFEGLNDVPKPTFNSMA
                     VTEPENFVEHFIDSTGVISWRFFSAKHDYEFCEWDFSGTNEEESACLFGILKDLGKEAY
                     VATFDNLGASACRILVPDYSEVYPVEDLIWDNTNKALAYREDILNIHSLSDDALSSLVE
                     RLEESQLDNYIDIRTLIGIEFDENTVWGQLTILELKILIYLALGELESAIELVEEFLQY
                     NDNTVERGLFYQAIHTVLEIALDEELELEHFIANLRRMFGEENMDNVVGSVTGEVRFFG
                     LTKTSMNLEGLDKHLRLIESYKKLHQARRVLAEK"
     misc_feature    complement(7515..7531)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            complement(7781..8785)
                     /locus_tag="SG34_RS15055"
                     /old_locus_tag="SG34_014990"
     CDS             complement(7781..8785)
                     /EC_number="3.1.1.-"
                     /GO_function="GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /locus_tag="SG34_RS15055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_014990"
                     /product="lipase family protein"
                     /protein_id="WP_236701259.1"
                     /transl_table=11
                     /translation="MGTSGGIMAHYQQKSTGFALIGKGQSSFYSKDYVIAFRGTVSRSD
                     ISTDIRATGSVGDNGSTVHAGFNTLFHTMKGELYAYFNHVRELSTIHCVGHSLGGALAS
                     LAADWFKYNFRHDIKLYTFGAPKVGYSNFALKTTNTLKQENIYRCCNGSDPIPMVPVWP
                     FVHAPYNGYEYSFAAGAIMNPFAHKLSRYEKNAKSESWDDYPRKRSFNSYNRVRLKPEN
                     SWQASPTMYWYVRLSEALVTLIRDVRIGLLNSIQSGIANGLNLYDQIAMVLSDVSFATP
                     SFNIQINGLLAHMLVFSGLAAVKITELSYEFIRWIFQKTLAVLNRVARQALSL"
     gene            complement(8963..9424)
                     /locus_tag="SG34_RS15060"
                     /old_locus_tag="SG34_014995"
     CDS             complement(8963..9424)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF042847.2"
                     /locus_tag="SG34_RS15060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_014995"
                     /product="DUF6795 domain-containing protein"
                     /protein_id="WP_053046749.1"
                     /transl_table=11
                     /translation="MFGLLFKYTIDLCPEIRGRMTYKGKPITNLEVSRSLTYSDEDEEV
                     DTTITDNDGFFSFPKAQMKSRLPASIFHEPVIRQVLVLNYQDKNYLVWHFTQKQIKPHL
                     AIVNILKQLDVELTNSEIYHDFENIEEPGKLHTAVGICRWENDFKDTQP"
     gene            complement(9650..10795)
                     /gene="tssA"
                     /locus_tag="SG34_RS15065"
                     /old_locus_tag="SG34_015000"
     CDS             complement(9650..10795)
                     /codon_start=1
                     /gene="tssA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018691746.1"
                     /locus_tag="SG34_RS15065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_015000"
                     /product="type VI secretion system protein TssA"
                     /protein_id="WP_044839216.1"
                     /transl_table=11
                     /translation="MRLATAEQSADEYIQHYFGYTLTQLLAPIGEDQVGESVRHNGVYF
                     NIKQARESDDPTLPMGVWTHELKTADWLKVRELALDALAQKSKDLQLGVWLFEASIHIK
                     GFAGIAPATFLIQELCEKYWSNMHPEMVDGDIEYRTNALNWLNKKLLPVLGLIPITSAQ
                     LDGEEYCWNDWESACHYDKLKNQQQIDTEWDGPTPQSIKQRLAATSPDELLKLVYQLED
                     GLQALSHLQDWLDNRCGNDSPNLSDISELLRRVDDMLSKELMRRGVSLAREQERELFAA
                     AGEEGGTGGADQAPGDMGKPGGSGSGDGTIRDRSDAFICLRKAAEFLMQDDPHSPVPYL
                     VYTACEWGEKSAPDLYQELFLAKGGQLNIFEIMGLDVEREH"
     misc_feature    12152..12168
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 23.8"
                     /tool="antismash"
ORIGIN
        1 acactaccct aaacgagctg agagccaaca ggcgttcctg cgccattacg acgtgcccgc
       61 cgatgatccc cgcctgaacg gccagcaagg catttacagc gcgaaaattg ccggtcctgc
      121 caatgaaaac gcccatgtga tcatgctcga tgcccgctat ttccgctcgc cgaccttcgc
      181 cagctatggc cagtgcgaag gcgacaattc taccatactc gggcagcagc aatgggcatg
      241 gttgcaggcg gaatttaaca aaaaatccga ggtaaaaatt attgccagcg gcatacaggt
      301 gctgccgccg ttaaatctgg agcgtaataa gtccagttat tgtgcctatg gcgacggcaa
      361 attgttcgac caggcgatca gcaatctgca ggagcaggat atgtcgggca ccaactatga
      421 atcctgggca gaaatgccgc agcagcgtga aaaactgctg cgcatggcgc aaaagtcgat
      481 gaatgacggc aatgctaagg ccgttatctt tgtctccggt gaccagcact ggggcgagct
      541 gctgcaaaaa gatatgccgg cttctggcaa gcacggcaag gcggtaaccc tgtatgaagt
      601 caccgcttcg ggattcggcc aaaactggcc ctatcatatt cccaacccgc tgcgcctccc
      661 ggtttatgcc gataccgcgg gcaacggcaa ttacgatacc cagtgcaagc tgccgtttaa
      721 atacggcggc attgaataca agggctgtat tacccgggat cacgacaagc cctggtgtta
      781 tacccaaaca gacaacagcg gtaaaggcat tgcaggaaaa tggggcaact gtgctcccag
      841 cggcgcaacc attccaatcg gtaaagtcgg tgttgtgtca gccaatattg ccggtttaac
      901 cacagatgac cgccacctgg taaacaagtc aggcagcaat tacggtaaga tagatatcga
      961 ctggcaaaaa cgcaccataa aaatggcgat agaaaccgcc accgaggaag ccgtttctac
     1021 cgtgattaat ttttaagtcc ggctaaagcc gtaaataatg ctccgggaag cgggcataac
     1081 tgtttgcttt ccgggcttta ccagttgtaa aaattcccat tagccgggaa atgtctccat
     1141 tctggccaca ttttctaaat tgtcgtccca gttggccttg ctggcaacaa agatatgggc
     1201 gtctggctta ttggaaactt taacatccag gctgccggct ggaaccacca gtaattgtcc
     1261 gtccatctga acattgggca ttgccgagcc gcaagtcagg caaaagcttt taacatgtcg
     1321 ggtttgggga agattaaagg tttggatatt gtcctgcccg gataaccagt tcagcttggc
     1381 acttgttgaa aacaggctgg cggcatgagc cgagccggtg tccttctggc aatactggca
     1441 gtggcagaga aagaaattgt caaactcccc tgttatctca aaactcacct tgccgcaaag
     1501 gcaagaacct gtttttattg cactcatgtt atcttcctga acctaagtta aaacaagtga
     1561 taaatcgtaa ttttttcagt gagtaatggc aatagttatt tctttttctg tgacaggctg
     1621 ttgcgccagt ggtaagccaa tgttaagacg caacagtctg ttttaattgc agctagccag
     1681 ttttctcctg ttattcatca tgcttaggtg tgatcatcag ttgttccgga tttatcgtaa
     1741 tcagcacatt ttgcccgggg gtgaaacctg tcttacgcaa ccactttccc ctgagcacaa
     1801 tgcagggctc caggtcgacc ggcacatagt taatgccgat gcaatgggct ttagacggcg
     1861 cctcacaggt agtctccaac acggtaagtc tgcgataagt gggatatttt acttttgccg
     1921 aggcaagctc tggcgtatga tgacaatcag ccattacgta ctcctttgaa gtgcgtggtg
     1981 gttagcaacc tctgtgtgtt acagcactca ggggttgcga ctcttgttgc tgtgtgcccg
     2041 gagttcaggg cgggcaagga atgctcatat gaaccacgag tctatctgaa atacataaac
     2101 aaggcagcag gagctcaatt gtttgtggct atatctggtt gtgcagatat atgagtctga
     2161 gcggcagtga cttatcgtat gctgataagg tgatgtaact cgctgacaaa ggggagtgag
     2221 gcgaattcgt tacgccaaga tgcttctcta gactaagtac tggacggatg aagaacatga
     2281 accgattaac ccgcatctat gggctgaaaa gtcgccactg cctacacgaa ttatcaggca
     2341 gtatgatgtt gtaataacgc tattagcagc agctttatta ccgatctgat aagtaactgg
     2401 taggtgctca tctgtagata aacgtttata acacaattct acgctttaaa catcatcacc
     2461 ttacagcatg ataaggtcaa agactgcgat cggcatccac cccaatatgt ttgagttcag
     2521 ataggtaaac cggggaaggt ttgtttggat gttaagggag catgacccca atgacagata
     2581 agctggcgga gcgtccgtag tagtttgggg tggggaaagc ccactacatg gcgaagggac
     2641 gcagtttaat tgatttgcgt aggcaaatca cctgacctta ataaggtgaa gacctttgat
     2701 aatcagcgaa ctgcaacgta agttagcaac atggacaaaa accgataaaa ctcgacgagt
     2761 taatcggctg ttacgtctta tcagccaccc acaatggctt tgcaaagcgg ctgaagtaac
     2821 gctttcctcc aaaggtgcca aaacaccagg tgttgatggc atgactaaaa ttcacttcca
     2881 ggcaaaatta gatgattatc tcactgaaat caggagcgat ttattatcag gtgattatca
     2941 acctatgccc gccagaagga tttacattcc taaagcaaac aaaaagcaac gccctctagg
     3001 tattcccacc ttgagagaca ggatagtaca acgcggcatg ctcatggcaa tggagccgat
     3061 atgggagaat gattttcact cattatctta cggctttaga cctgagcgca gtgtccacca
     3121 cgccattcgt acggttaagt tacagctaac cgatacaagt gattcaaaag gacgctgggt
     3181 gattgaaggt gatctatcaa gttattttga tacggttcat catcgattat tgatgaaagg
     3241 agtgcgaaag cgcatcaatt gtcagcgatt taatgaactg ctctggcgat ttattaaggc
     3301 tggccacata gaacgtaatt tattctgtgc aacaagcgag ggagtaccac aaggcggcgt
     3361 catatctcca ttactgtcaa atatcatgct caatgagttt gatcagtatt tagataaatg
     3421 ttacctaagc aagaaagcac gtaaagaccg ctggaactgg aaccacagta tcaagagcaa
     3481 acgtaaggtt gcaatcgatg aaaacaggca atggaaaccc gctgttgctt attgtcgtta
     3541 tgccgatgac ttcctagtga ttgtcaaagg caataaacaa caagcggagg tcattcgcga
     3601 tcaatgtcga gacttcctgg aaggtaaact aaagctcaca ttaaatatgg ataaaactca
     3661 tattacccat gtgaatgatg gttttatttt tttaggacat aggatcattc gaaaacgggg
     3721 gcccaaagga aatatgcgtg tggtatctgg tatccccaag gataaggcga aagcattctc
     3781 ttactcatta agtaaagcat tatccggaga ttatagttgt agcaagatag ataaagttga
     3841 gcagcttagc cggaaattaa aaggctgggc acaattttat cgacatactg attatacctc
     3901 ccaagtctat cgcaaaattg atcgtatcgt tttctggaaa cttgcaaaat ggctggctcg
     3961 gaaataccgt tgttccatta agtcacttat gattaaatgg atcaaacgac cgacgcctag
     4021 tcaggcaaag acctgggtgt tattcggtaa aagtaaccga ggtaatttat gcggcgcttc
     4081 actgtttcga ttggtgagca gccctaaatt acaatttcgt tggcgattgc ctgaatacaa
     4141 cccttacctt cgagatgaaa tgagaaatac ggtcacatct cgctatagcg atgttgcaat
     4201 ggctgttagc cacaattaaa cggagagccg tatgcgctga aaggtgcacg tacggttcgg
     4261 agaggagaag cagagaaata gttcgactac gctttgcctc ttactctacg cctgcattgc
     4321 ggcagcgggt gatttaataa tctgagattg atgctctcct tctttgggtg gatcgcacta
     4381 taaataaaga cctcttcgct ggttaaatca acgaaagagg tttttttatt aagataaatg
     4441 tggaataaaa ggtttgtagc ttaaagtttg gaggaattta aattttaagg tgctgatata
     4501 tcaaagtttt ttaggttctt atcctagccc tgaaatgatt ccgggtgctt tttcccgcta
     4561 gtgccgattt cctcccaggg agcttttgaa tccacccaca agtgatgggc cactttcagg
     4621 tttttgcctc cttccgagat taacccggcg ggaacatgca tacgggcatc atcattagcc
     4681 ccgggcaagg gagagccgca atttttacaa aagctggttt gccagtcatg gccgggtttg
     4741 tgccaggtgt tgattaattt tgtgccggta agccagtgaa aatcatcatt agcaatgacc
     4801 agcacggcaa tgccgccgct gccagtagat ttgcggcaaa tggagcagtg acaaatatag
     4861 acatctgaaa tttcagtggt tatttcgaat gttaccgcgc cacagttgca attccctttt
     4921 gtcataaatc cctctaaaca tgtgttggcg tgaactttag ccttttcagg aaaaagctct
     4981 gcgccaacgt attcattatg ttacttttct gctaacaccc tgcgtgcctg gtgaagcttt
     5041 ttatagctct caatcagtct caggtgcttg tccagcccct caaggttcat gctggtcttg
     5101 gtcaggccaa agaaccttac ttcacctgtt accgagccga ccacattatc catattctcc
     5161 tcgccgaaca ttcttctgag gttggcgata aagtgttcaa gctccagctc ttcgtccagg
     5221 gcaatttcaa gtacggtatg aattgcctga tagaacaagc cgcgctctac ggtattgtcg
     5281 ttatactgca ggaattcttc taccagctca atggcagatt caagctcgcc caatgccagg
     5341 taaatcagga tttttagctc aagaatcgtc agctggcccc aaacggtatt ttcatcgaac
     5401 tcgataccga ttaaggtgcg gatatcgata tagttgtcca gctggctttc ttccaggcgc
     5461 tctaccaggc tgcttaaggc gtcatcgctt aaagagtgga tgttgaggat atcttcacgg
     5521 taagccagtg ccttgttggt gttatcccag ataagatctt ccaccggata aacttccgaa
     5581 tagtcaggta ccagaatacg gcaggcggaa gcgcctaagt tatcaaaagt ggcaacgtag
     5641 gcttctttgc cgagatcttt taaaataccg aacaggcagg cgctttcttc ttcgttggtg
     5701 ccggagaagt cccactcgca gaattcatag tcatgcttgg cgctgaagaa acgccaggag
     5761 atcacgccgg ttgagtcaat aaagtgctca acaaagtttt ccggctcggt taccgccatg
     5821 ctgttaaagg ttggcttggg cacgtcgttt aagccttcaa aactgcggcc ctgcaacagc
     5881 tcggtcaggc tgcgctctaa tgctacttca aagcttgggt gggcgccaaa agaggcaaag
     5941 acaccgccgg ttcttggatt catcagggtg acgcacatca ccgggaactg gccgcctaag
     6001 gaagcgtctt taactaccac agggaaacct tgtgcttcca gtccctgaat gcccgctaaa
     6061 atacccggat atttctccag cactgccata ggtacgtccg gcagcacgat ttcctgttcg
     6121 atgatttgtc ttttcactgc gcgttcgaag atttccgaca aacactgtac ctgggcttca
     6181 tgctggttgt taccggcact catgccgtta cttaagaata aattctcaat caggtttgac
     6241 gggaaaaata ccgtttcgcc gtcggaatgg cgtacataag gtatcgaaca gataccgcgc
     6301 tcgacattac ctgagttagt gtcgattaaa tgcgagccgc gcagctcttt gtccggatca
     6361 taaatgccga gactgtagtc gtctaaaatg cccgccggca gctcgtcatt cggccccggt
     6421 ttgaaccatt cttcatgggg ataatggaca aaatcgctgt tggcgatctc cggaccaaaa
     6481 tactggtcgt tgtagaagaa gttgcagttc aggcgctcga taaattcgcc taatgccgaa
     6541 cataatgcgc tctctttggt tgcgccctta ccattggtaa agcacatagg cgatgccgca
     6601 tcacgaatgt gcagcgacca gacattagga acaatgttac gccaggaaga aatctcgatt
     6661 ttcatgccga gcttggccaa aatgccggtc atattggcga tggtttcctc aagcggcagg
     6721 tctttaccta aaatgtacgt actggcatca ccttccggtt gggccataag catggcctgt
     6781 gcatcttctt ccaggctttc aaccgcttca accttgaact cgggaccggt ttgcaccact
     6841 ttttttacgg tacagcggtc gatggatctt aagatcccct ggcgatcttt ttcggaaatg
     6901 ctttccggca gctccacctg aatctggaaa atctggttat agcggtcttc cggatcgaca
     6961 atgttgttct gggacaagcg gatgttgtca gtcggaatat cccgggccgc acaatacacc
     7021 ttgacaaaat aagccgcaca aagggcggaa gaagccagga aatagtcaaa cgggctggga
     7081 gccgagccgt cgcccttata gcggatcggc tggtcggcaa taacagaaaa atcatcaaac
     7141 ttagcttcaa ttctaaggtt gtcgagatag ttaactttga tttccatcga gtggtttccg
     7201 aagttgttcg ctgcaactgg aagcgtttaa acctttttgt tgaggtttta acggctgccc
     7261 gttagcggat tgatcttaat tggggaggga ttatccttgt ttttgcggca atagtcttgg
     7321 ataaatttca taaattggcg aaattgatgt tttcagaggg gaaattgttg gtaggaaatc
     7381 taatggctat ttaaatttat ctcaagacat aagatgttga aaaaaattga acatctccag
     7441 ctcttttgag agataacctc aggttatatc gacggtttat gaagctccta agtatcgcaa
     7501 tatttccgtg ggagataccc tagccctgta ttatccctac ccggtttatt tttttgtctc
     7561 taaaggcaat gagcgcctgg gagaaagaat tgaacaagga ttaaaacaaa tattacagat
     7621 cggctcgtta aaaaggcttc tcttaatatc atctgcatga tactaagaga agccggttac
     7681 ataaacgtaa aattttacag ctggaaaatc agctgctgct cgaagggaca cccgcctccg
     7741 gcagttcctg atggttacag gcaacggcag agccgaaagt ttaaagtgac aacgcctgcc
     7801 tggctacccg gttaagtacc gccagagttt tctgaaatat ccagcgaata aattcatagc
     7861 taagttcggt tattttcact gcagctaatc ctgaaaatac cagcatatgg gctaacaagc
     7921 cattgatctg aatattaaaa ctaggggtgg caaagctaac atctgacagc accatggcga
     7981 tttgatcata cagattcaag ccattggcta tgccactttg aatggagttg agtaaaccta
     8041 tgcgcacatc gcggattaag gttaccaaag cttcacttaa acgcacatac caatacatag
     8101 tcggactggc ctgccaactg ttttctggct ttaacctgac ccggttgtaa ctgttaaagc
     8161 tacgctttct cggataatca tcccaacttt cacttttggc atttttttcg tacctgctca
     8221 acttatgggc aaagggattc ataatagcgc cagcggcaaa gctatattca tagccattat
     8281 acggcgcatg aacaaaaggc catacaggca ccataggtat agggtctgaa ccattgcagc
     8341 agcggtagat attctcctgc ttcagggtgt tagtggtttt cagggcaaaa ttactgtaac
     8401 ccactttagg cgcaccgaag gtatataatt taatgtcatg tctgaaatta tatttaaacc
     8461 aatcggctgc cagggatgcc agtgctcctc ccaggctgtg accaacgcaa tgaatagtac
     8521 ttaactctct aacatgatta aaataagcat acagttcccc tttcatggta tgaaacaggg
     8581 tattaaaacc ggcatgtacg gttgagccgt tgtcgccgac actgccagtt gcgcgaatat
     8641 cggtggatat atcactacgt gatacagttc cccgaaatgc tatgacataa tcttttgagt
     8701 aaaaactact ttgccctttg cctattaaag caaaccccgt acttttctgc tggtaatggg
     8761 ccataatgcc acctgaggtt cccattatcg gacctttact ccaatcaaag gtaaaatgat
     8821 ctctaatttt ctggcggcag aacaattaaa tacgagtttc caatgtgagg tttttgaatt
     8881 ttatatgcca aatcagcaag ttgtacggcc ttgtagggtg agagtgttgc cataatttat
     8941 cctttcatgg ttaaactttt acttacggct gtgtatcttt aaagtcattt tcccaacggc
     9001 aaatgccaac agccgtatga agttttcctg gctcttcaat attttcaaaa tcatgataaa
     9061 tttcagagtt ggttaattcc acatcaagct gtttaagaat attaacaatg gccaaatgag
     9121 gtttaatttg cttctgggta aaatgccaaa ccaaataatt tttatcttgg taattcaaaa
     9181 ctaatacttg ccggataaca ggctcgtgaa aaattgaagc gggtaaccgt gatttcatct
     9241 gtgcttttgg aaaagaaaaa aagccatcat tgtcagttat tgtagtatca acttcttcat
     9301 cttcatcgga ataagtgaga ctacgggaaa cttccagatt agttatcggc tttcctttat
     9361 atgtcattct gccccggatc tcaggacaaa ggtctatcgt gtatttaaac aacaaaccaa
     9421 acataaaata aattcctttt tattcttttg ctcaagttga tagtgcaata cacaagtatc
     9481 aatgccatat ccgggaaatg caacctgccg ggaaatagtc gcagcggtaa tgttattcat
     9541 tttatggtgc cttggctatg catcagacaa atccaaccaa tcgcggttgt tacaggtaaa
     9601 cacaaaatca ggcgtcatgc tgaacgtgta tataaagtaa cacatcattt taatgttccc
     9661 gctccacatc cagccccata atttcaaaaa tattcagctg tccgccttta gccagaaaca
     9721 actcctgata aaggtcaggt gccgattttt ccccccattc acaggcggta taaaccagat
     9781 aaggcacagg actgtgggga tcgtcctgca tgagaaattc cgccgccttg cgcaggcaaa
     9841 taaaggcgtc actgcgatct cttatggtgc cgtctccgga accgctgccg ccgggttttc
     9901 ccatatcacc gggcgcctga tctgcgcctc ccgtgccgcc ctcttccccg gcggcagcaa
     9961 acagctctct ttcctgctcc cgtgccaggg atacgccacg gcgcatcagc tctttgctaa
    10021 gcatgtcatc aacccggcgc agcagctcac tgatatcact caggttcggg ctgtcgttgc
    10081 cgcagcggtt atccagccag tcctgcaaat ggcttagtgc ctgcaagccg tcttccagct
    10141 gatacaccag tttaagcagt tcatccggcg atgttgccgc caggcgctgc ttgatgcttt
    10201 gcggtgtcgg tccgtcccat tcggtatcta tctgctgctg gtttttcagt ttgtcgtaat
    10261 ggcaggcgct ttcccagtcg ttccagcagt attcttcacc gtcaagctgg gcggaagtga
    10321 tggggatcaa gcccagcacc ggaagcaatt ttttattgag ccagtttaag gcattggtgc
    10381 ggtattctat gtcgccgtct accatttccg gatgcatgtt gctccagtat ttctcgcata
    10441 attcctggat taaaaaagtt gccggggcaa ttccggcgaa accctttata tggatgctgg
    10501 cttcaaacaa ccagacccca agctgcaggt ctttactttt ttgagccagg gcatccaggg
    10561 cgagttctct taccttgagc cagtcggcgg tttttaattc atgggtccat acccccatgg
    10621 gcagcgtggg atcatccgac tcccgggcct gcttaatgtt aaagtacacg ccgttatggc
    10681 gcaccgactc ccctacctgg tcttcgccaa tgggggccag cagctgggtc aaggtatagc
    10741 cgaaataatg ctggatatat tcatcggctg actgctctgc tgttgctaat ctcatattcg
    10801 cacccttgtt atttatcggc taatcaatca ttaaagcccg gggcataagc aggtaaagtg
    10861 ccgggcaaag gtaatttttt cacatccccc tgcttgtcca tgacctcgac ccataccagt
    10921 agattggacc ggcttaaata ggccggcttt tgttcctgct ttccgggctt ggggggagtt
    10981 tttaactgca cgggtacatg gaatgccagc agggtttcat cctggcggct ggcttttaaa
    11041 ccgttcatgc ctttgctggc atatctggta agccattcaa ataatcccca ttttccctgg
    11101 cttttaaaag tggcattcag ttgtttctga tccacggtta aaggcggatt aagccgggga
    11161 tttacctctg tggttaacaa tgggatcaag ggcttaaatg ccgaacctgt tgcccagcgc
    11221 aaatccaggc ttaaaggttc gcctacctgc cacaagagct tattttgacc attggggaaa
    11281 ctggccagct gctggccgct ttgcatacgc cagtcgatga tctggtcgct gcccttggca
    11341 tattggggta aagcagagaa ctctatagtc aggggaacct gccactggtt ttgtttgctg
    11401 ttccagcttt gattgaaaaa gtcactgatg ctgttcatct ggctgataaa ctcgcgccag
    11461 gatgccggca gggcttcacc tgctaccctg cttttcgatg atttagccgt ttgtgaccgg
    11521 ctctccttag ttttgtcttt agttccgctg cttccggctg ccgccgttga ctcggctatg
    11581 gtttcatctt cttgcaagcc attgagggtg ttcatatctg ccagcaggtt tttcgatgcc
    11641 ttgcggtaat agcctaagaa agtttgcagc tggttgatac tgacctctcg aacacccgct
    11701 ttgtcaatcg aactaaaggg gaaacggccg gcaatttgct gattaaacct gtctcgtatc
    11761 gccagatagc ggttgataat ttctttatcg cccgcatcca ggcattgcac gcttacctgg
    11821 tgttgcaatt gcagcctgcg gatcacaaac cagccgttgt tggcggcttt attgtcaaaa
    11881 ttacttttct cataacaatt actcgtggtt aatcccgcca gggtatcgct gacaaaattt
    11941 tccagcgcca gggtctggtt ccccggctcg ttacgttgat aacggccgag atcacttaag
    12001 gtagcaaacc agcgctgtgc cagtgcatca cttaatcccg agctgttttg taaaaaacca
    12061 atcagaggct cggcatagtt gaaggcaaga taactaagcc tctgccgctg attggtgagg
    12121 taactggtaa tctgcttgtc tgtgtccagg ctgaataaaa tgcgggcaaa gtcccggtta
    12181 tcccagc
//
