LOCUS       NZ_CP059733            12977 bp    DNA     circular CON 26-MAR-2024
DEFINITION  Thalassomonas viridans strain XOM25 chromosome, complete genome.
ACCESSION   NZ_CP059733
VERSION     NZ_CP059733.1
KEYWORDS    RefSeq.
SOURCE      Thalassomonas viridans
  ORGANISM  Thalassomonas viridans
            Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
            Colwelliaceae; Thalassomonas.
REFERENCE   1  (bases 1 to 6726747)
  AUTHORS   Pheiffer,F., Schneider,Y.K., Hansen,E.H., Andersen,J.H.,
            Isaksson,J., Busche,T., Ruckert,C., Kalinowski,J., Zyl,L.V. and
            Trindade,M.
  TITLE     Bioassay-Guided Fractionation Leads to the Detection of Cholic Acid
            Generated by the Rare Thalassomonas sp
  JOURNAL   Mar Drugs 21 (1), 2 (2022)
   PUBMED   36662175
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726747)
  AUTHORS   Olonade,I., van Zyl,L.J. and Trindade,M.
  TITLE     Draft Genome Sequences of Marine Isolates of Thalassomonas viridans
            and Thalassomonas actiniarum
  JOURNAL   Genome Announc 3 (2), e00297-15 (2015)
   PUBMED   25883295
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6726747)
  AUTHORS   van Zyl,L.J., Busche,T., Ruckert,C., Kalinowski,J. and Trindade,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2020) IMBM, University of the Western Cape, Robert
            Sobukwe, Cape Town, Western Cape 7550, South Africa
REFERENCE   4  (bases 1 to 6726747)
  AUTHORS   Olonade,I., van Zyl,L.J. and Trindade,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2015) IMBM, University of the Western Cape, Robert
            Sobukwe, Cape Town, Western Cape 7550, South Africa
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8; Canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 107.2x; 129.1x
            Sequencing Technology             :: ONT; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000948985.2-RS_2024_03_26
            Annotation Date                   :: 03/26/2024 01:21:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,855
            CDSs (total)                      :: 6,705
            Genes (coding)                    :: 6,644
            CDSs (with protein)               :: 6,644
            Genes (RNA)                       :: 150
            rRNAs                             :: 11, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 117
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 26 of 61
            Pseudo Genes (incomplete)         :: 44 of 61
            Pseudo Genes (internal stop)      :: 12 of 61
            Pseudo Genes (multiple problems)  :: 18 of 61
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:09:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2435078
            Orig. end                         :: 2448055
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059733.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12977
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="5"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12977
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12977
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7977](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7977)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     tRNA            832..908
                     /anticodon=(pos:2435944..2435946,aa:Asp,seq:gtc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SG34_RS10915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SG34_010850"
                     /product="tRNA-Asp"
     gene            832..908
                     /locus_tag="SG34_RS10915"
                     /old_locus_tag="SG34_010850"
     tRNA            935..1011
                     /anticodon=(pos:2436047..2436049,aa:Asp,seq:gtc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SG34_RS10920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SG34_010855"
                     /product="tRNA-Asp"
     gene            935..1011
                     /locus_tag="SG34_RS10920"
                     /old_locus_tag="SG34_010855"
     tRNA            1058..1134
                     /anticodon=(pos:2436170..2436172,aa:Asp,seq:gtc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SG34_RS10925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SG34_010860"
                     /product="tRNA-Asp"
     gene            1058..1134
                     /locus_tag="SG34_RS10925"
                     /old_locus_tag="SG34_010860"
     tRNA            1180..1256
                     /anticodon=(pos:2436292..2436294,aa:Asp,seq:gtc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SG34_RS10930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SG34_010865"
                     /product="tRNA-Asp"
     gene            1180..1256
                     /locus_tag="SG34_RS10930"
                     /old_locus_tag="SG34_010865"
     tRNA            1344..1420
                     /anticodon=(pos:2436456..2436458,aa:Asp,seq:gtc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SG34_RS10935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SG34_010870"
                     /product="tRNA-Asp"
     gene            1344..1420
                     /locus_tag="SG34_RS10935"
                     /old_locus_tag="SG34_010870"
     tRNA            1489..1565
                     /anticodon=(pos:2436601..2436603,aa:Asp,seq:gtc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SG34_RS10940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SG34_010875"
                     /product="tRNA-Asp"
     gene            1489..1565
                     /locus_tag="SG34_RS10940"
                     /old_locus_tag="SG34_010875"
     tRNA            1634..1710
                     /anticodon=(pos:2436746..2436748,aa:Asp,seq:gtc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SG34_RS10945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SG34_010880"
                     /product="tRNA-Asp"
     gene            1634..1710
                     /locus_tag="SG34_RS10945"
                     /old_locus_tag="SG34_010880"
     tRNA            1807..1883
                     /anticodon=(pos:2436919..2436921,aa:Asp,seq:gtc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SG34_RS10950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SG34_010885"
                     /product="tRNA-Asp"
     gene            1807..1883
                     /locus_tag="SG34_RS10950"
                     /old_locus_tag="SG34_010885"
     gene            complement(1967..4015)
                     /locus_tag="SG34_RS10955"
                     /old_locus_tag="SG34_010890"
     CDS             complement(1967..4015)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1061: methyl-accepting
                     chemotaxis protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /locus_tag="SG34_RS10955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_010890"
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="WP_044842166.1"
                     /transl_table=11
                     /translation="MLTDEIAFFSSLKGRIILKLILPAIGITALIVIINASNSFSSARD
                     NAEKILRLSVDKIALEIQRRNDKAVNTAKLMVLAQEEALFGNRKQSSDFARRVLAEHPE
                     FTGAYFGYEPDADGQDEKFKNSDTAGKSTDEQGRYLPYWYLDDNNNAVVTPLVDMETSL
                     YYGGLKEQFEKSKQAQVLVTEPYVYQGKMIVEQVYPISRNGRFTGIGGVDRALDAIDGL
                     LAEIKKETGRDLFLLSRGGRFIASTLATVQLNTKEVSVTAYAELLGRFYQKREQDLLEL
                     TRDPLDNKAYYFAGKLIPTGEWLLVLRESEQQVLGEIKGQLIQTIIIAAIGLAILIALS
                     LWFINSISKRVKSAMIKAEHVAVGDLSGHTACQSQMHDEIDAMEESLDKVTESYSKICS
                     LCGAIAGGDFGVSMEKRSEQDSVAESINSMSQRRREIEQAVTARSDQINTSIQTQSIEI
                     ENVASSMNQMSATVSEVANLATESADSAHQAASSVKEVQGQLSGALDEVQTLSREIGSA
                     SQAIGKVAASSENINQIVDVINMIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRNL
                     AAKTRDSTEEINDLIRQLENNVSSSVTIVENGLERTKRSVDRTEEANSSLVRVTEMIDS
                     ISDHMMQVATAVEEQSSTCEEINRNITVIHDASTQLKNFSEQGIEQN"
     gene            complement(4238..4513)
                     /locus_tag="SG34_RS10960"
                     /old_locus_tag="SG34_010895"
     CDS             complement(4238..4513)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SG34_RS10960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SG34_010895"
                     /product="hypothetical protein"
                     /protein_id="WP_044842165.1"
                     /transl_table=11
                     /translation="MSDSIPPDYQEQINRLSSDCIRLCDEFNKFSEECAFICDAFAAVT
                     RDPDDISPDTSEGIGHISYWLKYQVKDYREKIDELHQGLSSLRRQT"
     misc_feature    4673..4690
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 17.92"
                     /tool="antismash"
     gene            complement(5001..6260)
                     /locus_tag="SG34_RS10965"
                     /old_locus_tag="SG34_010900"
     CDS             complement(5001..6260)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010362869.1"
                     /locus_tag="SG34_RS10965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_010900"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_044842164.1"
                     /sec_met_domain="DAO (E-value: 2.5e-64, bitscore: 216.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKQYDIVIAGGGAIGAACAYFLSREKDLKIALVDYKKPGNASRA
                     SAGGLWAIGESVGLGCGVIFFKQMTQQRAEAEGVDLPPMRPHILPECFFEFALKSNDMY
                     PALWQELKDKHGVDFKFEKTGLKYIMYDKFDKLYADSIVEQIPGLAEQINWLSAEELAV
                     DEPHINADAIGALEFACDHQVNPYRLNEAYLEAARQNGVELFLQTHVTGVEKQGNKVTA
                     VVTDSGTLHCDTMINAMGAWAAELSEMATGTRIPVTPVKGQIILSERLPKILKGCVSTT
                     DCYIAQKDNGEVLIGSTTEEKGFDTTNTLPEIKELANGAMRCIPELKDMNIKRCWAGLR
                     PGTPDELPILGKMAGTEGYINACGHFRTGILTSAITGKLINDLYRGQPLDIDLTPFEYA
                     RFLEQENEQEQVQEAEQALV"
     gene            complement(6260..7657)
                     /locus_tag="SG34_RS10970"
                     /old_locus_tag="SG34_010905"
     CDS             complement(6260..7657)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010362868.1"
                     /locus_tag="SG34_RS10970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_010905"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_044842163.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.9e-24, bitscore:
                     83.8, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 1.5e-10, bitscore:
                     39.7, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTESKHGVVIVGGGTAGISAAAELAQLGIKSVVIDEAPRIGGPVF
                     RGPFREAISLPHLDDNLCQKIADIRELYQKNQQYIELRLNTRVLGPSGDSALLARHQDE
                     VFEIPYDQLIVATGCHERSVPFDGWQMPGVMLLGGVQLQLKSGLVRPGQRMAVVGTGPL
                     LPLVATQLHKAGVNVTGVFEAGTLGDFAKESLAFLNRPMLALEGVGLLTYLKTNGIPMH
                     YGMGIVSAGGEDSLSSITVAQYDKDWYPIKGTEKTLDVDCVAVGYGFVSRNQLTQLLNL
                     SHENCQLSGLKPQVDDWQHSSRPDVYVAGDSVGILGGEAAMMEGRLAALGIARQLHILS
                     PEQAKDKGKPYKNLLDKVKAFRAGFDRIGRRREGLLSLLKPDTIVCRCENVTRAQIDEV
                     VEQGVKDITSLKMRTRAGMGDCQGKTCNSYCQDRLRFELKQLDVGEIKPRFPLDPVPFS
                     SLYQEEL"
     gene            complement(7654..7977)
                     /locus_tag="SG34_RS10975"
                     /old_locus_tag="SG34_010910"
     CDS             complement(7654..7977)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /locus_tag="SG34_RS10975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_010910"
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="WP_044842162.1"
                     /sec_met_domain="Fer2_4 (E-value: 1.1e-16, bitscore: 59.0,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESKEKLPRSQDVVPPNGQFIPVWVDGTQVDGIPGESILSVLFAV
                     GKRSITKNDHGKVTGAYCGMGVCHCCTVKVNNQHKVKACQTLIEPSMEIDTQSNRLDEG
                     GMA"
     misc_feature    complement(8204..8217)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.36"
                     /tool="antismash"
     misc_feature    8259..8276
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.09"
                     /tool="antismash"
     misc_feature    complement(8294..8314)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 23.99"
                     /tool="antismash"
     gene            8478..10298
                     /locus_tag="SG34_RS10980"
                     /old_locus_tag="SG34_010915"
     CDS             8478..10298
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008304810.1"
                     /locus_tag="SG34_RS10980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_010915"
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="WP_044842161.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 3.3e-13, bitscore:
                     48.7, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.1e-18, bitscore:
                     65.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 3.3e-22, bitscore:
                     77.7, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSYKAPIRDIKFLFEDVFDYYGHYQKYPEYAEATPDLVDAIFAE
                     CAKFSENELLPLNQSGDKEGCHFDNGVVTTPKGFKEAYRQYVEGGWQSLSHPVEQGGQG
                     LPPSMGMVKSEMMGTANWSWGMYPGLSHGAMNTIQEHGSDEQKEVYLTRLTEGTWTGTM
                     CLTEPQCGTDLGQVKTKAVPNGDGTYNITGTKIFISAGEHDLTENIVHIVLARLPNAPA
                     GTRGISLFIVPKMQVDQQGNLGEGNNVTCGSIEDKMGIKASATAVLNFDNAKGVLIGPE
                     NKGLECMFTFMNTARVGTALQGICTAELAYQNSLLYAKDRLSMRSLSGKKAPEKVADPI
                     IVHPDVRKMLMTQKAISEGGRAMIYYAAKLVDEIESAKTEAERKAADDRLGFITPILKA
                     FLTELGCESASHGMQVFGGHGYIKEWGMEQIVRDARIATLYEGTTGIQALDLLGRKILL
                     TRGVALKDFAMEILGFCKDHSMISGYSHKRQMNKFIWPLSKAVANWQQYSLRIALKARK
                     DRDFIGACSVDYLMYSGYIVMAYFWAKMAQAAYEKLASGVEDRDFYRAKIKTAEFYFER
                     MLPRTRSLAKTMMADPKTLMQLDGELLSFL"
     gene            complement(10417..11307)
                     /locus_tag="SG34_RS10985"
                     /old_locus_tag="SG34_010920"
     CDS             complement(10417..11307)
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013752596.1"
                     /locus_tag="SG34_RS10985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SG34_010920"
                     /product="acyltransferase"
                     /protein_id="WP_044842160.1"
                     /transl_table=11
                     /translation="MLSALPGFVIFPLTFVLFCLNLSFCGSLVFLGGLLKLLLPTPRAR
                     KMLYQPMHMIYRLWAWGNFAIMSLFNNIHWQIRGGESLNKQSWYLLIANHQSWFDIMVL
                     AHFARSRIPEPKFFLKEDLRKVPFLGMACWALDMPFMKRYSKSFLEKRPHLKGKDIETT
                     KNSCSHFKHQPTTIINFVEGTRCTPEKQRAQNSSFKHLLPPKAGGIAFTLATLGSQFNK
                     VLNITLVYPDNSGHVMMDMLTGKLKEVVIEIEQLPVSEEIIGDYFGDAGFKTRFQQWLN
                     DRWQVKDQLIEKLSR"
     misc_feature    11436..11450
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 20.87"
                     /tool="antismash"
ORIGIN
        1 aaactgccga tggcaaccag ggagctcagg caagcgccgg ttattttgca tccgaccccg
       61 gtgaccatag aggtggatac caccaatgaa gaagaggcct ttcataaagt acactttgtt
      121 atcgatgaca gctttgtcga tcccgacagt ttaattttcc agggcaattt tgtttttccc
      181 gaccgccagg ttgaagcctt ttctattatg gaaggcagcg gcggcgagcg tatcaaggag
      241 ctggccttta ccgagccggg catttaccgg gtaaaaaccc gggcgtttgg ccgcacgaaa
      301 acgggccgcg agtttcgcct ggtgattccg gaatttgcct ttaatgttga acctaaagca
      361 gacgacatta actttaccgt agatgaaaac ggacaaacga agaagttaac ccaggaagaa
      421 atcgcccagc agcaagcgga gcaaagagcc tttgaattgg agcaggaaag aataaggcaa
      481 gaagaggaag cggccgaaag gcagcagcaa atgatcacta ttattgtggt cagcaacctg
      541 gtgattcttg taattgccgt tgcagccttc tttatcctca gatggcgtaa gaaaaagccg
      601 gctaaacagg aagctaaaca ggaagaagcc taggacgccg ttaatctttg ccgttaaatt
      661 ttaagcaaaa gcgcccttta gcggcaaaaa atgcacgtta atcgagcgct ttgtttagca
      721 aatgcgcagg cgaaaaataa tatgaaaaaa gaagttgacg gatacacttc ataaccgtat
      781 tatctccgcc gcattcaacg agttgttgtt gaaacgcaga gtttaagtcg cggagtggta
      841 gttcagttgg ttagaatacc ggcctgtcac gccgggggtc gcgggttcga gtcccgtcca
      901 ctccgccaat ttaaacctgt tattgcatct cttaggagtg gtagttcagt tggttagaat
      961 accggcctgt cacgccgggg gtcgcgggtt cgagtcccgt ccactccgcc accgatgcaa
     1021 acatataaca gctttttgta catgattaag atatcgcgga gtggtagttc agttggttag
     1081 aataccggcc tgtcacgccg ggggtcgcgg gttcgagtcc cgtccactcc gccacatttt
     1141 agtcgccaat gcatttgaaa agtagtacag atatatcgcg gagtggtagt tcagttggtt
     1201 agaataccgg cctgtcacgc cgggggtcgc gggttcgagt cccgtccact ccgccacatt
     1261 cctctatgct tctttcgggt ttgtttatca aactatccaa ggttttgaga atccataaaa
     1321 tctctaatgt aaagctatat cgcggagtgg tagttcagtt ggttagaata ccggcctgtc
     1381 acgccggggg tcgcgggttc gagtcccgtc cactccgcca ccttgcttta cctggttatg
     1441 ggagccagcg ccgttaccgg taaaatctca aacgtaaagt tatatcgcgg agtggtagtt
     1501 cagttggtta gaataccggc ctgtcacgcc gggggtcgcg ggttcgagtc ccgtccactc
     1561 cgccacctta ctttacctgg tcatgagagc cagtactgtt accggtaaaa tctcaaacgt
     1621 aaagctatat cgcggagtgg tagttcagtt ggttagaata ccggcctgtc acgccggggg
     1681 tcgcgggttc gagtcccgtc cactccgcca ccttacttta ctcaggttac ccttttctta
     1741 caggtaattt ttcggagctg agaaccggaa ctttctcaag tgaaactcag cacaagtaga
     1801 tatcgcggag tggtagttca gttggttaga ataccggcct gtcacgccgg gggtcgcggg
     1861 ttcgagtccc gtccactccg ccacttttac ttaaattcac cgtcttcttt ctttatgctc
     1921 tttaccttca agtaagctca atctcgatat attttgtccc ttacttttag ttttgttcga
     1981 taccctgttc gctgaagttt ttcagttgcg tggatgcatc atggatcact gtgatatttc
     2041 tgttgatttc ttcacaggtg gaactttgct cttccactgc ggttgcgacc tgcatcatgt
     2101 ggtcgctgat gctgtctatc atttcggtca cccggactaa agagctgttg gcttcttctg
     2161 tgcggtcaac actgcgtttg gttctttcca ggccattttc aacgatagtc acagagctgc
     2221 tgacgttatt ttccagctgg cggatcaaat cattgatttc ttccgtggag tcgcgggttt
     2281 ttgccgccag gttgcgtact tcgtcggcta ctacggcaaa gccgcgtccc tgttcgccgg
     2341 cacgggccgc ttcgatagcg gcatttaatg ccagcaaatt ggtttgttcg gcgatcatat
     2401 tgatcacgtc gactatctgg ttgatgtttt cgctgctggc ggccaccttg cctatggcct
     2461 gacttgccga acctatctcc cgggataagg tctgaacttc atccagggcg cccgataatt
     2521 gtccctgcac ttccttgacc gaacttgccg cctgatgggc actgtcggcg gattcagtcg
     2581 ccaggttggc aacttcacta acggttgctg acatctggtt cattgaggag gcaacatttt
     2641 ctatttcgat actctgggtc tggatactgg tattgatttg atccgagcgg gcagtgaccg
     2701 cctgctctat ttcccggcga cgttgtgaca tgctgttaat agactccgcc actgagtcct
     2761 gctcgctgcg cttttccatg ctgacgccaa aatcaccgcc ggcaatagcg ccgcataaac
     2821 tgcatatttt actgtaggat tccgtgactt tgtccaggct ttcttccatc gcatctatct
     2881 catcgtgcat ttgagactgg caggcggtat ggcccgataa gtcacctacg gcaacatgct
     2941 cggctttgat catggccgat ttcacccgtt tgctgatgga gttgataaac cataaagaca
     3001 aggcgataag gatggccagg ccgatggcgg ctataataat cgtttggatt aattgccctt
     3061 taatctcccc caggacctgt tgctccgact ctctcagtac caacagccat tcgcctgtag
     3121 ggattaattt gccggcaaaa taataggcct tgttatccag gggatcccgg gtcagctcca
     3181 ataaatcctg ctcccgtttt tgataaaaac ggcccagtaa ctcggcataa gccgttacgg
     3241 aaacttcttt ggtatttagt tgtacagtcg ccagcgtact tgcgataaag cgtccgccgc
     3301 ggctgagtaa aaataaatcc cgtccggttt cttttttgat ttccgccagt aagccgtcaa
     3361 tggcgtcaag ggctctgtca acaccgccta tgccggtaaa ccgaccgttg cggctgatgg
     3421 gataaacctg ctctacgatc attttcccct ggtacacgta aggttcggta accagaacct
     3481 gggcttgctt tgatttttca aattgttctt ttaagccgcc gtaataaagg ctggtttcca
     3541 tatcaaccaa cggagtgacc acagcgttat tgttgtcgtc aagataccag tagggcaggt
     3601 agcgtccctg ctcgtcagtg cttttaccgg ccgtgtctga atttttaaat ttttcgtcct
     3661 ggccgtcggc atcgggttca tagccaaaat aggctccggt aaattccggg tgttctgcca
     3721 gtacccgccg ggcaaaatcg ctggattgtt tgcggttgcc aaacagtgcc tcttcttgtg
     3781 ccagtaccat taatttagcg gtattgacgg ccttgtcgtt gcggcgctgt atttcaaggg
     3841 caattttatc taccgagagc ctgagtattt tttcggcatt atcccttgcc gatgagaagg
     3901 aattgctggc attaatgatg acaatcaggg cggtaatacc tatggccggc aaaattaatt
     3961 tcaggataat ccggcctttg aggctggaaa agaaggctat ctcgtctgtt aacataataa
     4021 ctaccttagt ttaaagctac atgagatatt acggcttgtt taaagatagt ttatttagcg
     4081 gtaattctcc ggcaaaacat ggttgctatt aacagctctt atctggcgct tgaattggtt
     4141 tattgaataa cggatgggaa gttgtgcttt gggaataggg cggagcaatt aatgctgata
     4201 tcgactccgg cccttgttct tttagcggga cttgtcttta agtctgtcgc ctgagggaag
     4261 acaaaccctg gtggagttcg tctattttct cccggtagtc tttaacctgg tatttgagcc
     4321 aatagctgat atggcctatg ccctcactgg tgtccggact gatgtcatcg ggatcgcggg
     4381 taacggcggc gaaagcatca caaataaagg cgcattcttc gctgaacttg ttaaactcgt
     4441 cgcagagcct gatgcagtcg ctcgacaacc ggtttatttg ctcctggtaa tccgggggaa
     4501 ttgaatcaga catggtgcca tcctccctta aatgtttgta gcagggcgct gccgggaaag
     4561 cgtgggctgc ttgcggacgc aggtggggag acaggtatat tttctgttaa agaaacttta
     4621 accgagtgaa actctggcat atcattaggt gtagccataa tggatacctc tcttgtttag
     4681 tatctgttac ggttagcttc ctctgagtgg tcggacactc ggggggagcg cttcgtgtgt
     4741 caacaatgtc ttctgtatta gttaactaat accttaatta atacctcctt tggttaagct
     4801 ttttatcaag ctcattaagt aatagtacaa aatagcggat aaatccgctt ggggaggcat
     4861 atcatttttc gtggatttgg agctagtgta tagattactg tacacaaaga aaacggtctg
     4921 ttagctgggc tgtaaattac ggagaaaaag aaaggaaagc cggggctttc ctttgcttga
     4981 ttgcgggttg gtaattttta ttaaaccaga gcttgttctg cttcctgaac ctgttcctgc
     5041 tcgttttcct gctccagaaa acgggcgtat tcaaacggag tgaggtctat atccagaggc
     5101 tgtccccggt agagatcgtt tatcagcttg cctgtgatgg cagaggtcaa tattccggta
     5161 cggaaatggc cgcaggcatt gatataacct tcggtacctg ccattttccc caaaataggc
     5221 agttcatccg gcgtgcctgg gcgtaagcct gcccagcaac gcttgatgtt catgtcttta
     5281 agctcgggga tacagcgcat ggcgccgttg gccagctctt tgatttccgg cagggtattg
     5341 gtggtatcga agcctttttc ttccgtggtg ctgccgatca gcacttcgcc gttatctttt
     5401 tgcgcgatat aacaatccgt ggttgagacg cagcctttaa gaattttcgg caaacgctcg
     5461 gacaagatga tctgaccttt gaccggggta accgggatac gggtacctgt ggccatctcg
     5521 cttaattcgg cggcccaggc gcccatggcg ttgatcatag tatcgcagtg cagggtgccg
     5581 gagtctgtca ctacggcggt gactttattg ccctgttttt ccacgccggt gacatgggtt
     5641 tgtaaaaaca gctcaacacc gttttgccgc gcggcttcca ggtaggcttc gttcaggcgg
     5701 taggggttga cctggtgatc gcaggcaaac tccagtgcgc ctatggcatc ggcattgata
     5761 tggggctcgt ctaccgccag ctcttcggcg ctgagccagt tgatttgctc cgccagcccc
     5821 gggatttgct cgactatgga gtcggcatag agcttatcga acttgtcgta catgatgtat
     5881 ttcaggccgg ttttttcgaa cttgaaatcc accccgtgct tgtctttcag ctcctgccat
     5941 aaggccggat acatgtcgtt gctttttaag gcaaattcaa agaagcattc cggcagtata
     6001 tgcgggcgca tcggcggcag gtcaacgcct tcggcttcgg ccctttgctg ggtcatttgt
     6061 ttaaaaaaaa tcaccccgca gcctaagccg accgattcgc caattgccca caggccgccg
     6121 gcagaagcgc gtgaggcatt gccgggcttt ttataatcca ccagggcgat tttcaggtct
     6181 ttttccctgc tgaggaaata ggcgcaggcc gcgccgatgg cgccgccacc ggcgattacg
     6241 atatcgtatt gttttttcat tataattcct cctgatataa tgaagaaaag ggcacaggat
     6301 ccaacgggaa cctgggtttg atttctccaa catccagctg tttgagttca aagcgcaacc
     6361 tgtcctggca atagctgttg caggttttgc cctggcagtc tcccatgcct gcccgtgtac
     6421 gcatttttaa tgaggtgatg tctttgaccc cctgttctac cacttcatct atctgggcgc
     6481 gggtgacatt ttcacaacga cagacaatag tgtccggttt taataacgac aacaggccct
     6541 cccggcgtct gccgatgcgg tcgaaaccgg cgcggaaggc tttgacttta tcgagcaggt
     6601 ttttataggg cttgccctta tcctttgcct gctccgggct taggatatgc agctgccggg
     6661 cgatgcccaa tgccgcgagg cggccttcca tcatggcggc ttcaccgcct aaaataccga
     6721 cgctgtcacc ggcgacataa acatccggcc tgcttgaatg ctgccagtcg tcaacctggg
     6781 gttttaaacc gctgagctgg cagttttcat gcgacaaatt caataactgg gtgagctggt
     6841 tgcgggaaac gaagccatag ccgacggcca cacagtcaac gtccagagtt ttctccgttc
     6901 ccttaatcgg ataccagtct ttgtcgtatt gggcaactgt gatgctgctt agggagtcct
     6961 cgccgccggc ggacactatt cccatgccgt aatgcatggg aataccgttg gttttcaggt
     7021 aggtgagtag tcccaccccc tctaacgcca gcatagggcg gttgaggaag gcgagacttt
     7081 ctttggcaaa atcccctaag gtacccgctt caaatacgcc ggtaacatta accccggctt
     7141 tatggagctg ggtggctacc aggggcagca aggggccggt gccgacgacc gccatgcgct
     7201 ggcccggtct gaccaggccg gatttcagct gcaactgtac gccgccaagc agcataacgc
     7261 cgggcatttg ccagccgtca aaaggcacgc ttctttcgtg gcagccggtt gcgactatca
     7321 gttgatcata ggggatttca aagacttcat cctggtggcg ggcgagcagg gcgctgtctc
     7381 ccgacggacc cagcacccgg gtattgaggc gcaattcgat atattgctga tttttttgat
     7441 aaagctcccg gatatcggca atcttttggc aaaggttgtc atccaggtgg ggcaagctga
     7501 tcgcttcgcg aaacggcccc ctaaataccg gaccaccgat cctgggggct tcatcaataa
     7561 caacagattt aatgccaagc tgggctaatt ctgcagctgc ggaaatgcct gcggtgccgc
     7621 ctccgacaat tacaacgcca tgcttagact ctgtcatgcc atgcctcctt catccagacg
     7681 gttgctctga gtatcgattt ccatactcgg ttcgattaag gtttggcagg ctttgacttt
     7741 atgctggttg ttgactttga ccgtacagca atggcataca cccatgccgc aataggctcc
     7801 cgtaacttta ccgtgatcgt ttttagtgat agacctcttc ccaacagcaa atagcacgct
     7861 aagaatactt tctccaggga ttccatcaac ttgagtgcca tcaacccata ccgggataaa
     7921 ttggccatta ggtgggacta cgtcctgact tctcggtagt ttttccttac tttccattgg
     7981 cttttcctta tcgttaaaca gccttactac tacgcttgtt cctaaacggg ttagaaaata
     8041 gcggacaact ggataatatt agattaagga ttgaaatcca acaactaaag tttggtattt
     8101 ctttgattta ggttaaggtt attattgttt ttagagtttt tgctttattc ttgggtttgg
     8161 ctttttacgt ttaaatgctg ttgattttca atgttttgtg ttgtttttgt aaaaaatatc
     8221 gtcactttaa aacatcaaaa cttgtttaaa taaccatctt gttaacattt tgtaacgtaa
     8281 tcgatgttaa tctaaaatat ccatttttat attttgtttc atatcagacg cttatagata
     8341 tagtgcatta attttcgtca gtggtccccg ggaggcagat ttaagcttga aatggtggta
     8401 tgaccagttg acttgagctg tattctcgtc acataatagt ttttatttat tcaattattt
     8461 ccgtcagggg tctacaaatg ctcagttata aagcgccaat aagagatata aagtttttat
     8521 tcgaagatgt gtttgattat tacgggcatt atcagaaata tcctgaatat gccgaagcca
     8581 ccccagacct ggtggatgct atttttgccg aatgcgccaa gttcagcgaa aatgagcttt
     8641 tgcccctaaa ccagagtggc gataaggaag gctgccattt tgataatgga gtagtgacta
     8701 cgcctaaggg ttttaaagag gcgtaccggc aatatgtcga agggggctgg cagagtttgt
     8761 cgcatcctgt agagcagggc gggcaggggt tgccgccgtc tatgggtatg gtgaaatcgg
     8821 aaatgatggg tacggccaac tggtcctggg gcatgtatcc ggggctgagc catggcgcca
     8881 tgaacaccat acaggagcac ggcagcgacg agcagaaaga ggtttatctg acccggctga
     8941 cggaaggcac ctggaccggc accatgtgtc tgaccgagcc tcagtgcggt accgatttag
     9001 gccaggtgaa aaccaaagcg gtacccaacg gcgacggcac ctataatatt actggcacga
     9061 aaattttcat ttccgccggg gagcatgatt tgacggaaaa cattgtccat attgtgctgg
     9121 cgcgcctgcc gaatgcgccg gccggcaccc ggggcatttc cctgtttatc gtgccgaaaa
     9181 tgcaggtgga tcagcagggg aacctggggg agggcaataa cgttacctgt ggttctattg
     9241 aagataagat gggcattaaa gcctcggcta ccgcggtatt gaactttgac aatgccaagg
     9301 gggtattgat agggccggaa aataaggggc tggaatgtat gttcaccttt atgaacaccg
     9361 cccgggtggg cacggcgctg caggggattt gtaccgcaga gctggcctac cagaattccc
     9421 tgttgtatgc caaagacagg ctgtcgatgc gttctttatc aggcaagaaa gcaccggaaa
     9481 aagtggccga ccctatcata gtacacccgg atgtccgtaa gatgctgatg acgcaaaaag
     9541 ccatcagcga agggggccgg gcaatgattt attatgccgc caagctggtg gatgaaatcg
     9601 agagcgcgaa aacagaagcc gagcgcaagg ccgccgatga ccgtttaggt tttattactc
     9661 ctatcctgaa ggcgttttta accgagttag gctgtgaaag cgccagccat ggcatgcagg
     9721 tttttggcgg ccatggttat attaaagagt ggggtatgga gcagatagtg cgcgatgccc
     9781 gtattgctac cttgtatgaa ggcactaccg ggatccaggc cctggatttg ctgggacgta
     9841 aaattctgct tacccgcggt gtggccctga aagattttgc catggaaatc ctgggcttct
     9901 gtaaggatca cagcatgatt tccggctata gccataagcg gcaaatgaat aagtttatct
     9961 ggccgttaag taaagcggtg gccaactggc agcagtattc gttgcgcatt gcccttaagg
    10021 ccagaaaaga tcgcgacttt atcggcgcct gttccgttga ttacctgatg tattcaggtt
    10081 atatcgtgat ggcctatttc tgggcgaaaa tggcccaggc ggcttatgaa aaactggcca
    10141 gcggagtgga agacagggat ttctaccggg caaaaattaa aaccgccgag ttttatttcg
    10201 agcgtatgct gccccggacc cggtcactgg caaaaaccat gatggcagat cctaagaccc
    10261 tgatgcagct tgacggcgag ttattgtctt tcctgtaggt tagtttatca gatatgaaaa
    10321 agccccgtta tcacctgatg gcggggcttt tttgatgcgt tatattgccg aacaggtttc
    10381 agtaacaagg agatattacg tgaattacct tctatcttaa cgggataact tttctatcag
    10441 ctggtctttg acctgccagc gatcgttcag ccattgctgg aaacgggtct taaagccggc
    10501 gtcgccgaag tagtcaccga taatttcctc ggataccggc agctgctcta tttcgataac
    10561 cacttcttta agtttgccgg tgagcatatc catcatgaca tgccctgaat tgtcggggta
    10621 gaccagggtg atgttcaaga ctttattaaa ttgcgatccc agggttgcca gggtaaaagc
    10681 aatgccaccg gctttgggcg gcagcaaatg cttgaagctg ctgttttgcg cccgctgttt
    10741 ttctggggta caacgggtac cttcgacgaa attgatgatg gtggtgggct ggtgtttaaa
    10801 atggctgcaa gagttttttg tggtctctat gtccttgcct ttgaggtgcg ggcgtttttc
    10861 cagaaaactt ttgctgtagc gcttcataaa gggcatatcc agcgcccagc aggccatgcc
    10921 taaaaagggc accttgcgca ggtcttcttt caagaaaaat ttcggctccg ggatacggct
    10981 gcgggcaaaa tgtgccagca ccatgatatc aaaccagctt tggtggttgg cgataagcag
    11041 gtaccaggat tgtttgttga ggctttcgcc gccgcggatc tgccaatgga tgttattaaa
    11101 taagctcata atggcaaagt taccccaggc ccataaacgg tagatcatat gcatgggctg
    11161 atacagcatt ttccgggcgc gtggcgtagg caagaggagt tttaacaggc cgccgagaaa
    11221 aaccaaagag ccgcaaaaac tcaggttaag gcaaaataag acaaaagtca ggggaaaaat
    11281 aacaaatccg ggtaaagcac tgagcatatc tggtgagaac ctcgcttgga atactgactg
    11341 aaaaggcgct actttaacga tatttgaaga aaaagggtac tggtttattt gttacatatg
    11401 tacgctgaat tgttgattct taagctattt ctttcatttt tcagccaata aattaatgat
    11461 ttaaaaaaat cgcataccgg tatccggtca taacggcatc acctgtattt tgcgcataaa
    11521 aaaccctgcc ggagcagggt ttcaggttta acgcttatat ggcgttaggc ggcgtgcggc
    11581 tgttttttag cttcatcctt tagcagggat ttgtccgcag gcaaatctgc cgggtcaaag
    11641 tcatcgacat ctatggcagc tttacggccg gtttcagcgc ggcgcaataa ttctgcttcc
    11701 gtctcattga tggcgccgca ggccaggccc tgctccgcta cttcgtttaa gcggtagaaa
    11761 ggcagcttag tgcccactgc acggcaaacc ttttcaaaaa tcggctcaca ggcgacgata
    11821 tcgtccaggg tttgttccag ctgacccatc aggttgctgc tttcgcgggc aaggtattgt
    11881 ccctcgccta agcggcttct ggcatcgctc ggggtttgca gcagacgggc aacctggtga
    11941 tctcttttat ctgacggttt ggttaaccag cagcctaacg gcagtaccag tacccttaac
    12001 gcccggccga cgattttatt cgggaagttg gtcagcatgg catccatggc ttgctggata
    12061 ttgtacaagc aatcttcaac cgcccattgc attaatggta agtcttcggc cctgcgtcct
    12121 tcgtcgttga aacgttttaa ggtggcagat gccaggtaca gataacttaa catgtctcct
    12181 aagcgggcag aaacacgttc tctgcgttta agatcaccgc ccagggtcag catagcgaca
    12241 tccgacaaca tggccaggtt ggagctgaag cgcgtcatta actggtagta acggcgagtt
    12301 tcgtcctgat aaggggtttt cactaaacgt gagccggaca gggagaacca caggctgcgg
    12361 aaaatattgc tgacggcgaa accgacatgg ccaaacaggg catggtcgaa atcatccagt
    12421 gcctgcttgc tgtctttgtt gccggccgcc tgtagctccg ccagtacata tggatggcag
    12481 cggatggcgc cctggccgta gatgatcatg ttacgggtca ggatattggc cccttctacc
    12541 gtgatggcaa caggcgcccc ctgataaccg cgggcaagat agttgttcgg tcccatacag
    12601 atgcctttac cgccgtggat atccatggag tcggtgacac aggcacgcat tttttccgtt
    12661 aaatggtatt tggcgatggc ggaaattacc gaaggtttct cacccaggtc gatggcgccg
    12721 gtagacatag tggttaccgc gtccatcagg taagcattac cgccgatacg gcccaatgct
    12781 tcttcgatgc cttccatttt acctataggc agtttgaact ggcggcggat acggctgtag
    12841 gcgccggttg ccatggcaag cgatttaacg ccgccggcac tggttgaagg cagggtaatg
    12901 gcacggccaa ccgataaaca ttctaccagc atacgccagc cctggccggc cattttcggg
    12961 ccgccgataa taaagtc
//
