LOCUS       NZ_CP010957            10414 bp    DNA     circular CON 21-MAR-2024
DEFINITION  Sphingobium sp. YBL2 plasmid 3pYBL2-3, complete sequence.
ACCESSION   NZ_CP010957
VERSION     NZ_CP010957.1
KEYWORDS    RefSeq.
SOURCE      Sphingobium sp. YBL2
  ORGANISM  Sphingobium sp. YBL2
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Sphingobium.
REFERENCE   1  (bases 1 to 322226)
  AUTHORS   Yan,X.
  TITLE     The analysis of one genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 322226)
  AUTHORS   Yan,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2015) College of Life Sciences, Nanjing
            Agriculture University, Weigang Street #1, Nanjing, Jiangsu 210000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2014
            Assembly Method                   :: Celera Assembler v. 8.0
            Assembly Name                     :: YBL2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000943805.1-RS_2024_03_21
            Annotation Date                   :: 03/21/2024 04:34:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,153
            Genes (coding)                    :: 5,086
            CDSs (with protein)               :: 5,086
            Genes (RNA)                       :: 66
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 25 of 67
            Pseudo Genes (incomplete)         :: 53 of 67
            Pseudo Genes (internal stop)      :: 4 of 67
            Pseudo Genes (multiple problems)  :: 12 of 67
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:45:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 284412
            Orig. end                         :: 294826
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010957.1.
            Bacteria available from yanxin@njau.edu.cn.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10414
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10414
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10414
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5414](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5414)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(200..1447)
                     /locus_tag="TZ53_RS24855"
                     /old_locus_tag="TZ53_24875"
     CDS             complement(200..1447)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1234: transporter,
                     small conductance mechanosensitive"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820586.1"
                     /locus_tag="TZ53_RS24855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_24875"
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="WP_044663537.1"
                     /transl_table=11
                     /translation="MITNLPVSNDSISTLTRGYVTEPWMQGGLAFLLLILFAWGANWVA
                     KRIVLRLLLRLLGQLPFHVEAKHIGAIVARLSNIVPALIIQGGIGAVPHLPVEAATFVR
                     SLCTAFIVLTIAVALNGFLALLNDLYQRRPDAAHRPIKGYVQLGKLLVYAAAAILIIAA
                     LMDQSPLLLLSGLGAMAAVLMLVFKDTILSLVASVQIGSNDMMRVGDWIEMPQFNANGD
                     VIDIALHTVKVQNFDKTITTIPTHRLISESFRNWRGMAESGGRRIMRSLMIDQGSVRFL
                     DEDDTDSMGRFSVLRPYLQSKQREIEQWNAEHGAHGTINGRRLTNIGTFRAYVLAYLQS
                     RRDIAQDKTLLVRQLAPSENGLPLEIYAFANSTIWAEYEGAQADIFDHLLAILPEFGLR
                     LFQRPAGADLAAMTAA"
     gene            complement(1444..2790)
                     /locus_tag="TZ53_RS24860"
                     /old_locus_tag="TZ53_24880"
     CDS             complement(1444..2790)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1074: major
                     facilitator family transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964706.1"
                     /locus_tag="TZ53_RS24860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_24880"
                     /product="MFS transporter"
                     /protein_id="WP_044663538.1"
                     /transl_table=11
                     /translation="MTAISSHRTVAASPTTAGDVTLRRAIAASAMGNATEWFDYGIYAY
                     GVTYISAALFPGPTDEAVLFALATFAISFLVRPLGGLFWGPLGDRLGRKSVLALTILLM
                     SGATLCVGLIPDHASIGLWAPALLIALRMVQGFSTGGEYGGAATFMAEYAPDDRRGFYG
                     SFLEFGTLAGFSLGAALMLGFSLMLGDTAMHAWGWRIPFLVAAPMGLVGMYLRSKMEDT
                     PVFRAAAALHDSGPPPSPGLPLLVRRHWRPMLVVGGLVVALNVVNYTLLSYMPTYLQRR
                     IGLTSDQALIVPIIGMLLMMAALPFAGALSDRVGRRTMWRMSLVGLLVAVVPLYMLMAT
                     GLIGAITGFLLLGLLYVPQLATISATFPALFPTQVRFAGFAIAYNVSTSIFGGTAPAIG
                     SGLIGLTGDELMPAYYMILACIVGLLALRFMPETAGHSLQDDPFQEKQA"
     gene            complement(2787..4034)
                     /locus_tag="TZ53_RS24865"
                     /old_locus_tag="TZ53_24885"
     CDS             complement(2787..4034)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820588.1"
                     /locus_tag="TZ53_RS24865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_24885"
                     /product="MFS transporter"
                     /protein_id="WP_044663539.1"
                     /transl_table=11
                     /translation="MFLAIKPVRSILLSIFMLMAGSGFLATLISLRLNRAGSGTMTIGI
                     VATAYFGGLIIGSLRAGGVVRRVGHIRAFAAFVALLSASTLSYVLLSHPLFWMLLRLVD
                     GMCVAGVFVCLESWLNDRAEPQMRGSVLAAYMVALYSGQAIGQLLLGASGALPAIPFQL
                     ASILISLAIIPLCLTRSAAPAPVEASAFSIRSLLAASPLGAWGAVATGLMLGAFYGLAA
                     VHVRRLGLDLPETARFMMFVILGGVALQWPLGRLSDRHDRRQVIIGSFAAAALVSMALS
                     LGQDGLWLTGLGALFGGLSFALYPLCVAHCNDRLLETERVAASGRLVLLYSLGAALGPL
                     LAAGAMTAFGTGGLFLFIALCAGLAMTVGLWRQRASEPVPVRYQQDFQVLPRTTPMASL
                     LDPNSADETLTQGQTT"
     gene            complement(4041..4976)
                     /gene="thpD"
                     /locus_tag="TZ53_RS24870"
                     /old_locus_tag="TZ53_24890"
     CDS             complement(4041..4976)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042345.1"
                     /locus_tag="TZ53_RS24870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_24890"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_044663618.1"
                     /sec_met_domain="PhyH (E-value: 6.5e-31, bitscore: 102.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQDIYPSRHAKMAEFLPRLDPVVHSAWSKDAPIGRDEAAQFERDG
                     YLVLENLFSSEEIAFLQSEAGQLLSDPDALEDETVISEPGSREIRSIFRIHAQSPVIAR
                     LAADNRLASVARFLLGDEVYIHQSRLNYKPGFQGKEFYWHSDFETWHVEDGMPRMRALS
                     MSVLLAENMAHNGPLMLIPGSHRSFLTCVGQTPEDHYRMSLKRQEYGVPDEDSLAELAH
                     RHGIVAPTGQPGSVVIFDCNIMHGSNGNITPFPRANAFLVYNALSNRLCAPFGVEKPRP
                     EFIAARGELQPIVPVSGSMTERTGMPGQAA"
     gene            complement(5001..5414)
                     /locus_tag="TZ53_RS24875"
                     /old_locus_tag="TZ53_24895"
     CDS             complement(5001..5414)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013022.1"
                     /locus_tag="TZ53_RS24875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_24895"
                     /product="ectoine synthase"
                     /protein_id="WP_044663540.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.1e-49, bitscore:
                     161.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRKLQDIRKSDRNVKSQQWESARLLLKDDNMGFSFHITTMYAG
                     EEIHMHYQNHLEAVLVLKGNGTIEDLGTGITHQLASGVMYALNAHDRHIVRPTTDILCA
                     CVFNPPVTGREVHDENGTYPAEADMIRASALSN"
     gene            complement(5425..6729)
                     /gene="ectB"
                     /locus_tag="TZ53_RS24880"
                     /old_locus_tag="TZ53_24900"
     CDS             complement(5425..6729)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (35-367). E-value: 2.7e-74.
                     Score: 242.1. Matches aSDomain:
                     nrpspksdomains_TZ53_RS24880_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820592.1"
                     /locus_tag="TZ53_RS24880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_24900"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_044663541.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.3e-79, bitscore:
                     261.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MITLPRPTGRKPDTAIYERRESAVRSYARVMPRQFNRAENVWMHD
                     NQGGRYLDFLSGCSTLNYGHNHPVLKQALLDYIAADGIAHGLDLHTDAKAEFLETLESL
                     ILKPRRLDYRAMFTGPTGTNAVEAAIKLARKVTGRELVIAFTNGFHGMTLGALACTGNA
                     AKRGGAGVPLAHVAHEPYDGYHGPEVDTAELLERRLADPSSGLDAPAAILVETVQGEGG
                     LNAASPEWLRRINAMAKRHGALMIVDDIQAGCGRTGSFFSFEGMGFTPDIVTMAKSLSG
                     MGLPFALTLFRPELDQWAPGEHNGTFRGNNHAFVTATAALRHFWSDRRFEQDIARRGAL
                     VARRLEAMAAQHGLSTRGRGMMRGIDVGSGDIAAAVTTACFERGLIIETSGAHDEIVKI
                     LSPLVIDDAELSAGIDILETCIGEALGAPYGVAAE"
     aSDomain        complement(5629..6624)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TZ53_RS24880_Aminotran_3.1"
                     /evalue="2.70E-74"
                     /label="TZ53_RS24880_Aminotran_3.1"
                     /locus_tag="TZ53_RS24880"
                     /protein_end="367"
                     /protein_start="35"
                     /score="242.1"
                     /tool="antismash"
                     /translation="NRAENVWMHDNQGGRYLDFLSGCSTLNYGHNHPVLKQALLDYIAA
                     DGIAHGLDLHTDAKAEFLETLESLILKPRRLDYRAMFTGPTGTNAVEAAIKLARKVTGR
                     ELVIAFTNGFHGMTLGALACTGNAAKRGGAGVPLAHVAHEPYDGYHGPEVDTAELLERR
                     LADPSSGLDAPAAILVETVQGEGGLNAASPEWLRRINAMAKRHGALMIVDDIQAGCGRT
                     GSFFSFEGMGFTPDIVTMAKSLSGMGLPFALTLFRPELDQWAPGEHNGTFRGNNHAFVT
                     ATAALRHFWSDRRFEQDIARRGALVARRLEAMAAQHGLSTRGRGMMRGIDV"
     gene            complement(6763..7242)
                     /gene="ectA"
                     /locus_tag="TZ53_RS24885"
                     /old_locus_tag="TZ53_24905"
     CDS             complement(6763..7242)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423999.1"
                     /locus_tag="TZ53_RS24885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_24905"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_084694453.1"
                     /transl_table=11
                     /translation="MRLRLPTAVDGPLVTKLVASCPPLDVNSAYCNLLQCTHFAQSCII
                     AEKAGQLMGWVSGYRPPSEPESFFVWQVAVAPAARGQGLAGHMIEALLARPSSAEVTHL
                     ITTVTNDNQASWALFEGLANRWRTRLERSPFFHQQTHFAGAHATEWLARIGPLPR"
     misc_feature    7315..7330
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.98"
                     /tool="antismash"
     misc_feature    7474..7488
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.21"
                     /tool="antismash"
     gene            7518..8006
                     /locus_tag="TZ53_RS24890"
                     /old_locus_tag="TZ53_24910"
     CDS             7518..8006
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964691.1"
                     /locus_tag="TZ53_RS24890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_24910"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_172668358.1"
                     /transl_table=11
                     /translation="MSSEVAVLTLRALRRVLRATEIGSRRLAATTGLTPSQWLVLREIG
                     SRDSATPSAIATALKFSQATITAIVDRLQQLGFVQRQRSEKDKRQFILRPLPPGVEALA
                     EAPDLLQTTFTERFAKLPQWEQAMILAAVERLAMLMDAQNIDAAPLLDSGLIDRSEPS"
     gene            8135..9052
                     /locus_tag="TZ53_RS24895"
                     /old_locus_tag="TZ53_24915"
     CDS             8135..9052
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820596.1"
                     /locus_tag="TZ53_RS24895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_24915"
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="WP_044663543.1"
                     /transl_table=11
                     /translation="MRAAAWKTIVKRIWVNSGRHNLGLLSAGVAFYVFLSFVPLLAAVI
                     MIYGLAADPATVARHMRTILEVVPADAARLIQEQLTQLTTSAQDKKGIGLLIALLVSIF
                     GASRASGAMIGSLNIIYERQDRRSFLKNTFLASALAGAAVLVGIIGLAAASMLSFAKGL
                     VAGIGPVGATAVQALTWLIAASLCSFAIGGMYRFAPDRSDVRWRWFSLGSCLATLLWLA
                     ATLGFGLYASRFGNYDATYGSLGAVVVLLMWLFVSAYAVLIGGLINAEIERQAPCDTSA
                     EPTEAGGQRMGAARALFQQQAHAR"
     misc_feature    complement(8151..8165)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.36"
                     /tool="antismash"
ORIGIN
        1 gcctcccatg agcgttgtcg cccatcgcga cccggttctg gtcggcggaa cagtctcggc
       61 catggcgcac cccatcgatg tggaaggtga agggctaacg gaaaacggac ccatactgtt
      121 ccgttcgagg ctgatttcac ccttgagcga acgagccgtc cggctgtgcg atgcaggttc
      181 ccgccagtgg gcccgacagt tacgcggctg tcattgccgc caggtcagcg ccagcggggc
      241 gctggaacag tcgcagcccg aattccggga ggatcgcgag cagatgatcg aagatatccg
      301 cctgggctcc ctcatattct gcccatatcg tgctgtttgc aaaggcgtag atttcaagcg
      361 gaaggccgtt ttcgctcgga gcgagctggc gcacgagcaa ggtcttgtcc tgtgcgatgt
      421 cgcggcgcga ttgcagatag gccagcacat aggcgcggaa ggtgccgatg ttggtcagcc
      481 gcctgccatt gatcgtgccg tgcgctccat gctcggcatt ccactgctcg atttcgcgct
      541 gcttggactg cagatagggc cgcagcacgg agaagcggcc catgctgtcc gtgtcgtcct
      601 cgtccagaaa acggacactg ccctggtcga tcatcagtga acgcatgatg cggcgtccac
      661 ccgactccgc catgccgcgc cagttgcgga agctctccga aatcagccgg tgcgtgggaa
      721 tggtcgtgat cgtcttgtcg aaattctgga ccttgaccgt gtgcagagcg atgtcgatga
      781 cgtctccatt ggcgttgaac tgcggcatct cgatccagtc gcccacgcgc atcatgtcgt
      841 tcgacccgat ctgtaccgac gcgaccagcg acaatatggt atccttgaat accagcatca
      901 gcactgcggc catggcgcca aggccagaca gcaacaggag cggcgactgg tccatgagcg
      961 ctgcgatgat caggatcgcg gccgcagcgt agacaagcag cttgcccagt tgcacatagc
     1021 ccttgatcgg ccggtgggcg gcgtccgggc ggcgctgata gagatcgttg agcagcgcga
     1081 ggaagccatt gagggcgacg gcgatggtca ggacgatgaa tgccgtgcaa aggctgcgca
     1141 cgaatgttgc cgcctcaacg ggcaggtgcg gcactgcccc gatgccgcct tgaataataa
     1201 gtgcgggcac gatgttcgac agccgcgcca cgatcgcgcc gatatgcttg gcctcgacat
     1261 gaaagggcag ctgtccaagg aggcgtagca aaagccgcag cacgatccgc ttggcgaccc
     1321 agttcgcgcc ccaggcgaac aggatcagca gcaggaatgc gaggccgccc tgcatccatg
     1381 gttcggtgac atagccgcgg gtcagcgtcg aaatgctgtc gttggaaacc ggaagattgg
     1441 tgatcaagcc tgcttctcct ggaaaggatc gtcttgcagc gagtggcccg ccgtctcggg
     1501 catgaagcgc agcgcgagca agccgacgat gcaggccagg atcatataat aggccggcat
     1561 cagctcgtcg ccggtcaggc caatgaggcc gctgccgatc gccggcgcgg tcccgccgaa
     1621 gatgctcgtg gaaacattgt aggcgatggc gaagcccgcg aagcgcacct gtgtcgggaa
     1681 caatgcaggg aaggtcgccg aaatggtcgc aagctgcggc acataaagca ggccaagcaa
     1741 caggaatcct gtgatcgcgc cgatcaggcc ggtcgccatc agcatgtaga gcggcacgac
     1801 cgcgaccagc aggccgacaa gcgacatccg ccacatcgtt cgccgcccaa cccggtcgga
     1861 cagagccccg gcaaagggca gggccgccat catcagcagc atgccgatga tcggtacgat
     1921 cagcgcctga tcgcttgtga ggccgatccg cctttgcaga taggtcggca tatagctgag
     1981 cagcgtatag ttgacgacgt tgagcgccac gaccagcccg ccgacgacca gcatgggccg
     2041 ccagtgccgc cgcaccagca acggcaggcc gggtgagggg ggcgggccgc tatcatgcag
     2101 cgccgcggcc gcgcggaaga ccggcgtgtc ttccatcttc gagcgcagat acatgccgac
     2161 cagtcccatg ggggccgcca ccaggaaagg aattcgccaa ccccaggcgt gcatcgccgt
     2221 atctcccagc atcagagaga aacccagcat cagcgctgcg ccgagcgaaa agcccgccag
     2281 cgtcccgaac tccagaaagc tgccgtagaa gccgcgccgg tcgtcgggcg catattccgc
     2341 catgaaggtc gcggcgccgc catactcccc gccggtcgaa aagccctgca ccatgcgcag
     2401 cgcgatgagc agcgccggcg cccataggcc gatgctcgca tgatccggga tcagccccac
     2461 gcacagcgtt gccccggaca tcagcaatat ggtgagcgcc aggaccgact tgcgtccgag
     2521 gcggtcgccc agcggccccc agaacagccc gcccagcggc cggaccagga aggaaatcgc
     2581 gaaggtcgcc agtgcaaaca gcacggcctc gtccgtgggg cccgggaaga gcgctgcgga
     2641 aatataggtg accccatagg catagatgcc atagtcgaac cattcggtcg cattgcccat
     2701 ggccgacgcg gcgatggcgc ggcgcaacgt gacgtcgccc gccgtcgtgg gggaggcggc
     2761 taccgtcctg tgactggaaa tcgcggtcat gtcgtctgtc cttgggtcag cgtctcgtct
     2821 gcgctgttgg gatcgagcag cgaagccatg ggcgtggtgc ggggcaggac ctggaaatcc
     2881 tgctgatatc taaccggcac cggctcggac gcgcgctggc gccacaagcc gacggtcatg
     2941 gcgagcccgg cgcacagcgc gatgaagaga aacaacccgc cggttccaaa ggcggtcatg
     3001 gcgccggctg ccagcagcgg cccgagcgct gcgccgaggg aatagagaag caccagccgt
     3061 ccgcttgccg ccacgcgctc ggtctccagc agccggtcgt tgcaatgggc cacgcacagc
     3121 ggatagagcg cgaagctcaa tcccccgaac agcgcgccga ggcccgtcag ccacaggcca
     3181 tcctggccga gcgacagcgc catgctgacc agcgccgccg ccgcgaagct gccgatgatc
     3241 acctgcctgc ggtcatgtcg atccgacagc cggcccaaag gccattgcag cgccacgccg
     3301 cccaggatga cgaacatcat gaacctggcg gtttcgggca gatccagacc cagccgtctc
     3361 acatgcacgg ccgccagccc ataaaatgcg cccagcatca ggcccgtcgc gacagcgccc
     3421 caggcgccga gcggcgaggc cgccagcagc gaccggatcg aaaaggcgct ggcctccact
     3481 ggcgcaggtg cggcactgcg cgtgaggcac agcgggatga tcgccagcga aatgaggatg
     3541 gaggcaagct ggaatggaat ggccggcagg gcgccgcttg cccccagcag caattggccg
     3601 atagcctggc cggagtagag cgccaccata taggcggcca gcacgctgcc gcgcatttgc
     3661 ggctccgccc ggtcgttaag ccagctttca aggcacacga acacgcccgc gacgcacatc
     3721 ccgtcgacca gccgcagcag catccagaac agcggatgcg acagcagcac ataggacagg
     3781 gtactcgccg acagcagcgc gacgaaggca gcgaaggcgc ggatgtgtcc caccctccgg
     3841 accacgcctc ccgcgcgcag cgacccgatg atcagcccgc cgaaataagc ggtggcgacg
     3901 atgccgatcg tcatcgtgcc gctgccggcc cgattcagcc gcaggctgat cagcgtcgcc
     3961 agaaagccgc tgcccgccat cagcatgaag atggacagca aaatgctgcg gacgggtttg
     4021 atggccaaga acattatgtc ttaagcggcc tggcctggca tgcccgtcct ttcggtcata
     4081 gagcctgata ccggcacgat cggctgcagc tcgcctcgcg ctgcgatgaa ttccggacgc
     4141 ggcttttcga ccccgaaagg tgcacagagc cggttgctga gggcattata gaccagaaag
     4201 gcgttggccc gggggaaggg agtgatattg ccgttcgatc cgtgcatgat gttgcagtca
     4261 aaaatcacga ccgaccctgg ctggcccgtc ggcgcgacga tgccatgcct gtgcgccagt
     4321 tcggccaggc tgtcctcgtc cggcacgcca tattcctgcc gtttgagcga catgcggtaa
     4381 tgatcctcgg gcgtttgccc gacgcaggtc aggaagctgc gatgcgatcc ggggatcagc
     4441 atcagcggcc cgttatgggc catgttttcc gccagcaata ccgacatgga cagggcccgc
     4501 atgcgcggca tcccatcctc gacatgccag gtctcgaaat cgctgtgcca gtagaactcc
     4561 ttgccctgga agccgggctt gtagttgagg cgcgactggt ggatgtagac ctcgtcaccc
     4621 aacagaaacc gtgcgacgct cgccagcctg ttgtcggcgg ccagtctcgc gatgaccggg
     4681 ctttgcgcgt gaatgcggaa gatcgaacgg atttcgcggc tgcccggttc gctgatcacc
     4741 gtttcatcct ccagcgcgtc cgggtccgac aaaagctggc ccgcctcgct ctgaaggaat
     4801 gcgatctcct cgctggaaaa cagattttcc agtaccagat agccgtcgcg ctcgaactgc
     4861 gcggcctcgt ccctgccgat tggcgcgtcc ttgctccacg cgctatgaac gactggatcg
     4921 aggcgcggca gaaactccgc catcttggca tggcgggatg ggtagatgtc ttgcaaggga
     4981 ctgccccctt tcatcttcgg tcaattactc agtgcagacg cgcggatcat gtcggcttcg
     5041 gccgggtaag tgccgttctc gtcatgtact tccctgccgg tgaccggcgg gttgaatacg
     5101 caggcgcaca ggatgtcggt cgtggggcgc acaatgtgcc tgtcatgcgc gttgagcgca
     5161 tacatgacac ccgacgcaag ctggtgcgtg atgcccgtgc ccagatcctc gatggtgcca
     5221 tttcccttca gcaccagcac cgcttccaga tggttctggt aatgcatgtg gatttcctca
     5281 cccgcataca tggtcgtgat gtggaaggaa aagcccatat tgtcgtcctt cagcagcaag
     5341 cgcgcgcttt cccactgttg cgacttcaca tttcggtccg atttacggat gtcctgcagt
     5401 ttgcgaacga tcatgatatc tcctttattc cgccgccacg ccgtagggag cacccaatgc
     5461 ttcacctatg cacgtttcca atatgtcgat gccggccgaa agctcggcgt cgtctataac
     5521 caggggggaa aggatcttga cgatttcgtc atgcgccccg ctggtttcga tgataaggcc
     5581 ccgttcgaag caggcggtgg tgacggcggc ggcgatatcg cccgacccga catcgatgcc
     5641 gcgcatcatg ccccggccgc gggtggacag gccatgctgc gccgccatcg cctccagccg
     5701 tcgcgcaacg agtgcgccgc gacgagcgat gtcctgctca aatcgccggt cgctccagaa
     5761 atggcgcagc gccgcggtgg cagtgacgaa ggcatggtta ttgccgcgga aagtgccgtt
     5821 atgttccccc ggcgcccatt gatccagttc aggccggaac agcgtgagcg cgaagggcag
     5881 gcccatgcct gatagcgact tggccatggt gacgatatcg ggggtgaagc ccatgccctc
     5941 gaagctgaaa aagcttcccg tacggccgca acccgcctga atgtcgtcca cgatcatcag
     6001 cgcgccgtgc cgcttggcca tggcattgat cctgcgcagc cattcgggtg atgctgcgtt
     6061 gaggccgcct tcaccctgca cggtttccac cagtatggcg gcgggggcgt caaggccgct
     6121 cgaaggatcg gcaagccgcc gctccagcaa ctcggcggtg tctacctccg gtccgtgata
     6181 gccgtcatag ggttcatgcg cgacatgagc gagcggtacg cccgcgccgc cgcgcttggc
     6241 cgcattgccg gtgcaggcga gtgcgcccag cgtcatgccg tgaaagccgt tggtgaaggc
     6301 gatcaccagt tcgcgcccgg taactttgcg ggcgagcttg atcgcggctt ccaccgcgtt
     6361 agtgccggtc gggccggtga acatcgcgcg atagtcgagg cggcggggtt tgaggatcag
     6421 gctttccagc gtttccagaa attccgcctt ggcgtcggtg tgcaggtcga ggccatgcgc
     6481 gatcccgtcg gccgcgatat agtcgagcag cgcctgcttg agcactggat gattgtgacc
     6541 atagttcaac gtcgagcatc cggacaggaa atcgagataa cggccaccct gattgtcgtg
     6601 catccacaca ttctcggcgc gattgaattg ccggggcatg acgcgcgcat agctgcggac
     6661 ggcggattcg cgccgctcat agatggcggt gtcgggtttg cggcccgtgg gccggggaag
     6721 ggtgatcatg tcgccaagtc tccttgaggg atggccgcgc ggtcagcgcg ggagcgggcc
     6781 gatgcgcgcc agccattcgg tggcatgcgc gccggcgaaa tgcgtttgct ggtggaagaa
     6841 tgggctcctc tcgagcctgg tacgccatct gttcgcgagc ccctcgaaaa gagcccagga
     6901 tgcctgatta tcattcgtca ccgtggtgat gagatgggtg acttctgcag atgacgggcg
     6961 cgcgagcagg gcctcgatca tgtgtccggc cagtccctgc ccccgcgctg ccggcgcgac
     7021 agcgacctgc cagacgaaaa aactctctgg ttcggacggc ggtcgatagc ccgacaccca
     7081 gcccatcagc tgcccggctt tttccgcaat gatgcaactc tgcgcaaaat gcgtgcactg
     7141 caaaagattg cagtaggcgg aattgacgtc gagcggaggg cagcttgcaa caagtttcgt
     7201 gactaagggc ccgtcaaccg ctgtgggaag acgcagccgg atctccgcta tcgcgccatc
     7261 gcgatggcgc gcgcgcttcc gctgcggcga tgaagccgcg tcggaccgtg tctcaaagat
     7321 aattcatctc ccgaagtata ttcttcgtat ttcaggcgcc cctctcttgg cttgaggcgg
     7381 aagataccgc attagactcg attttcaacc ctttggcgca cgatgtggtc ctttggccga
     7441 tgaaggagtt tatcaattgc tttggtttgc aaaggatggg attggccagg aaaggccatt
     7501 ttccctatgt ttccatcatg agttcagaag ttgccgtcct cacccttcgg gccctccgcc
     7561 gggtcctgcg cgccacggaa atcggcagcc gccggcttgc cgcgacgacc ggtctcacgc
     7621 cttcgcaatg gcttgtcctg cgcgagatcg ggtcgcgcga ttcggcgact cccagcgcca
     7681 ttgccaccgc tcttaaattc agtcaggcca cgatcaccgc aatcgtcgac cggcttcagc
     7741 aactcggctt cgtgcagcgg cagcgcagtg aaaaggacaa gcgccagttc attctgcggc
     7801 ccctgccgcc gggcgtcgag gctctggcgg aggcgcctga ccttctgcag accacgttca
     7861 ccgagcggtt cgcaaagctg ccgcaatggg aacaggccat gatccttgca gccgtcgaac
     7921 ggttggcgat gctgatggat gcgcagaata tcgatgccgc cccgttgctc gacagcggtc
     7981 tcatcgaccg gtccgaacct tcctgagggc aaccttcgca ggagaagggc cctcgccctt
     8041 ctccccgttc ccaccatgaa ccgaagtgga catgtctatg tcagaacagg gacgcgcggc
     8101 gcgccgaggc gctgcccccg ggacattgga gcccatgagg gcggcggcgt ggaaaacgat
     8161 cgtcaaacgc atctgggtaa acagcggacg gcacaatctc gggctgctct cggccggtgt
     8221 cgccttctac gtcttcctgt cgttcgtgcc gcttctggcg gcggtcatca tgatctacgg
     8281 ccttgctgcc gatcccgcga cggtcgccag gcatatgcgc accattctcg aggtcgtgcc
     8341 cgccgacgcc gcccggctca tccaggagca gctcacgcag cttacgacga gcgcccagga
     8401 taagaagggg atagggctgc tgatcgcgct gctggtctcg atcttcggcg cgtcccgggc
     8461 ttcaggcgcc atgatcggat cgctcaacat catctatgag cggcaggacc ggcgttcgtt
     8521 cctgaaaaac acctttcttg cgagtgcgct tgccggtgca gcggtccttg tgggcatcat
     8581 cggcctggcg gccgcctcaa tgctcagctt cgccaaaggg ctggtcgccg ggatcgggcc
     8641 agtgggcgca acggctgtgc aggcgctgac ctggctcatc gccgcatccc tgtgcagctt
     8701 cgccatcggc ggcatgtatc ggttcgcgcc cgacaggtcc gatgtccgat ggcgatggtt
     8761 cagccttggc tcctgccttg caactttgct ctggctcgcc gcgacgctcg gctttggact
     8821 ttatgcgagc cggttcggca attacgacgc gacctacgga tcgctcggcg cggtggtcgt
     8881 gctcctcatg tggctgttcg tttccgctta tgccgtattg atcggtggcc tgatcaatgc
     8941 cgagatagag cggcaggctc catgcgacac gagcgcggaa cccacagaag caggagggca
     9001 gaggatgggc gccgcccgcg cgctgttcca gcaacaggcg catgcccgtt aacgcgcatg
     9061 cgcccatcat ctgactgacc gcgacgcttg gcggcacact tgccgaagga aaacgatgcc
     9121 aggaaccagc ctttcactgc gtcatttacg gatattgttg cggcggcatg gcccggaggc
     9181 cctgatctgg cgccgccgca tcgccatttt gggcggcgct gtcctcaccg gccttgccgc
     9241 cttgctgttc gcgcgtctgg cggacgaggc gagcgaaatg tttgtcggtc tggcaaggcg
     9301 gtggccggcc gcacatctgt tgctgacgcc attgggcttt gccgctatcg tctggacaac
     9361 ccgcaagctt gcgcctgcgg caagcgggtc gggcattcca caagtgatgg ccgcctctcg
     9421 tgatcccgag catgggctca accggctggt ttcggcacgg accgccatcg tgaagctggt
     9481 catgactgtt gcggggctgc tgctgggagc ctcggtcggg cgcgagggtc cgaccgtcca
     9541 gatttctgca acgatcatgg cctatgcgca tcggctgatg tcggttccgt tgcgggcatc
     9601 ggtctatata gcgggcggcg cggcaggcgt ggccgccgcg ttcaatacgc ctctggccgg
     9661 ggtggccttt gccatcgagg agcttgcggc ggcctatgag cagcgcatgg ccctgctggt
     9721 gatggccgct gtactcatcg cgggcatggt gagtttgggg cttgccggcg actatgtcta
     9781 ttttggcgtc atgcggcaga cgctcaatgt ccatgaggcg cttgtggccg ctccggtcgc
     9841 cggcgtgatt ggcggcctgg cgggagcctg tttctcgcgc ttcatgctgg gcatgggacg
     9901 ctcgacaagg acgccgatac gctggatgcg ggaaagaccg gtgcggttcg cggcaggatg
     9961 cggactggta gtggcgctcc tgggcgccgc cacaggcctc acctggggga ccggctatca
    10021 gaccgctcat gctgtcatcg caggctcaga cgtgccgata tggttcgggg cggcgaagtt
    10081 cgcggcgaca ctcgccactg cggtttccgg gatcccggga ggtattttcg cgccgtccct
    10141 gtccgtagga gcggggatcg gcgacgtgct ccgcagcatg ttcccggcct atcctgccgg
    10201 ggcgatcgtc ctgctgggga tggtcgccta tttcacgggc gtcgtgcggg cgccgctcac
    10261 ggctgtcatc atcatttcgg aaacgacggc aagccgcggc ctgatgatgc cgcttctggc
    10321 gaccgcgctc atcgccgact tcgccgctca actgatcagc aaggagcgcc tctatcacgg
    10381 cctttcccgc cgcttcgaga gcagtcccga acac
//
