LOCUS       NZ_CP010954            20636 bp    DNA     circular CON 21-MAR-2024
DEFINITION  Sphingobium sp. YBL2 chromosome, complete genome.
ACCESSION   NZ_CP010954
VERSION     NZ_CP010954.1
KEYWORDS    RefSeq.
SOURCE      Sphingobium sp. YBL2
  ORGANISM  Sphingobium sp. YBL2
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Sphingobium.
REFERENCE   1  (bases 1 to 4766421)
  AUTHORS   Yan,X.
  TITLE     The analysis of one genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4766421)
  AUTHORS   Yan,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2015) College of Life Sciences, Nanjing
            Agriculture University, Weigang Street #1, Nanjing, Jiangsu 210000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2014
            Assembly Method                   :: Celera Assembler v. 8.0
            Assembly Name                     :: YBL2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina MiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000943805.1-RS_2024_03_21
            Annotation Date                   :: 03/21/2024 04:34:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,153
            Genes (coding)                    :: 5,086
            CDSs (with protein)               :: 5,086
            Genes (RNA)                       :: 66
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 25 of 67
            Pseudo Genes (incomplete)         :: 53 of 67
            Pseudo Genes (internal stop)      :: 4 of 67
            Pseudo Genes (multiple problems)  :: 12 of 67
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:45:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1243299
            Orig. end                         :: 1263935
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010954.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from yanxin@njau.edu.cn.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20636
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hserlactone"
                     /region_number="4"
                     /rules="Autoind_synth"
                     /tool="antismash"
     cand_cluster    1..20636
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="Autoind_synth"
                     /kind="single"
                     /product="hserlactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20636
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:10636](+)"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10636
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
     gene            130..1341
                     /locus_tag="TZ53_RS05885"
                     /old_locus_tag="TZ53_05885"
     CDS             130..1341
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1130: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954973.1"
                     /locus_tag="TZ53_RS05885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05885"
                     /product="IS256 family transposase"
                     /protein_id="WP_011950834.1"
                     /transl_table=11
                     /translation="MTDDRLPLAELMAKTGDGDFLRTIAESVLQIIMEADVDGLVGASR
                     HERSGDRTTWRNGYRDRSLDTRLGTLNLRIPKLRTGAYFPGFLEPRKTVEKALVAVIQE
                     AWIAGVSTRRVDELVQAMGMTGISKSSVSKLCKDIDERVHAFLKRPLTGDWPYLWLDAT
                     YLKVREGGRIVSVAAIIAVAVNTEGKREIVGLHIGPSEAEPFWSSFLKDLARRGLTGVK
                     LVISDAHEGLKAAITRVLSATWQRCRVHFMRNALAYVPKGQNTVVAAAIRQVFLQPDHA
                     AATQVWRQVADQLRTRWPKLGACMDDAEHDVLAYMTFPEQHRVKLHSTNPLERLNKEVK
                     RRADVVGIFPNEDSIIRLVGAVLLEQNDEYQLQHRYMQIEGMAALATPMIEEAQPLQIT
                     PKAA"
     gene            complement(1478..2050)
                     /locus_tag="TZ53_RS05890"
                     /old_locus_tag="TZ53_05890"
     CDS             complement(1478..2050)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959997.1"
                     /locus_tag="TZ53_RS05890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05890"
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_238320122.1"
                     /transl_table=11
                     /translation="MIGRCCATLLAGGFASTLIVQAAPAYGEARDLDMLAAEKAINQCI
                     HRAASGRPWLAQTLRALRKREGGWIGAEVRNRDGSVDLGPMQVNSWWVPRIARMIGRSE
                     SQIKSWLRDDPCFNVDAARWIFLSALHDVKDFWRAVGIYHSPTRWRQMRYSRSIASIML
                     IQRQAFVASPLRERKFRKSHLVIFGPR"
     gene            complement(2047..4977)
                     /gene="mobF"
                     /locus_tag="TZ53_RS05895"
                     /old_locus_tag="TZ53_05895"
     CDS             complement(2047..4977)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene="mobF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950760.1"
                     /locus_tag="TZ53_RS05895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05895"
                     /product="MobF family relaxase"
                     /protein_id="WP_044660684.1"
                     /transl_table=11
                     /translation="MIHPRRLSGSAGNIARYYTVGDYYTKGSDEHSEWAGKVAVELGLE
                     GAVDPTTFKELLAGRVEGRQLGRRRADGTIRHHPGWDFAVNAPKSVSIMALVAQDQRVL
                     EAHEQAVGEAISWLEEHAAYRFRWQGEIVHETTGRIVAARFTEHASRELDPHLHTHIVV
                     MNITDREDGAPMTSMETRAMFAEQMVAGQIYRNGLAQKLHAHGFELETDPHRGLFEIRG
                     VPKSLIADWSKRAEQIDAHAEEHGRKGQAARVASFYQTRKAKQKTTTEGLRERWDKEAG
                     PLLEMLHKVKGAADRDGVIDHEGSRAIAGRAALFGIRNMETREAVNSQGQLIRTGLAVH
                     VGEVSLRDVLPFVRDHEQREKLLTTRTQTGDALLTRGRTSRATARLEIALSKHLAMALD
                     DTRPLASTDRLLLVLEGEGLSSQQERALATLATSRDRLVGIHGVAGSGKSTLVRALVAT
                     VDADVRVQALAPTSSAAAELGKKAKIPSQTVMNLIVSGGRKLDARDLLVVDEAGQLSNR
                     QALRLLEVSRDTGARILFLGDNKQTGAIEQGKAFWLLQQLGLPKAELSESLRQQNNQKM
                     MKAVMKARLGEYARAIGFLDGVVSGDKASKLAKNLVDQWMQLAPETRAATNILVLDNAT
                     RITVNANVRRGLQKEGVIAIQEVPLKILAPAGLTSEERRMARLYSKGQVVMFNRDVDQL
                     GIARQAEYAVVGFGREESGREVVRLEDQQGRIILWDPRRTRASMVSVFNVQDRGLAKGD
                     RIQWRLATKELGLKNAERGTVERIENERATIRWDRTGERQEIDFSKYKTWDHGYAETVY
                     SSQSKTYPRVFVLAPVDSPLVTGQNFYTAITRAEFSVTLWTENRADLAEKLKRNSGQKT
                     SSLEGLDLIKRSGTEARLEREAARIQKEHDLYDVQRRELALLRTERRNRNRGSEPKGLA
                     GAGLRATETLTALIERMLRNQPQPEISQPKQPDRTTEVRRDRGRDR"
     gene            complement(4974..7028)
                     /locus_tag="TZ53_RS05900"
                     /old_locus_tag="TZ53_05900"
     CDS             complement(4974..7028)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823832.1"
                     /locus_tag="TZ53_RS05900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05900"
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="WP_044660685.1"
                     /transl_table=11
                     /translation="MSIFRNDTLGSWTRGGQAIVHNVRMTTQVFFQTMSACLVLWVMGI
                     LWYCFETLTAYQRYVLVQLALAEFRKDATRGAADPVQFQTPGGHKYWTTADALLNSGVA
                     RKTLEAGEAALITGALIGGIGAALALGWAWYYFTRTGRGLGSNQFLRGARFGTLRSIRR
                     LLRRYRKGSFTIGGVAMPHAFEPEHIVLCGAPGTGKTNIITTMLEGIRKNGKRAIVYDT
                     AGSFVEKFYRPGKDILLNPLDQRTDHWSPWVDVPREYHYDQIAQSTIPDKAGDPFWAKA
                     ARGTLVGVLRKLARQKHTLVSILLDRLLRSGLKDLVAFAEGTEAAAFISTEGDRTSAGI
                     QAELASVMRSFAYLDDTANGFSIRSWVENETDDSWLFITVKADQLPSLRPLITVWIDIA
                     IGAIMSMKPDPERRLYCIIDELPTLQRLPSLSDFLARARKYGGCGILGFQSYPQLEATY
                     GIQDAAAITGYCSTWIALRANDTPTAKHVSENLGQVEQIEANEGMSYGVNDMRDGVNLS
                     RMQVTRPLVMHTEVTNLPNLSGFLRFGRDLPVIRFADRFNPISNIAPAFVERTKPPIRL
                     PLADEIIQAARAAAEERAAQSGQAAGRIRKKAARKSGWVPPPQNAPRSEKSAPELPLGQ
                     TPEVSPPDRKQEKTTPPSPSMLGARAGARTVPLLRHGRVNEIPGEADPA"
     gene            complement(7025..7351)
                     /locus_tag="TZ53_RS05905"
                     /old_locus_tag="TZ53_05905"
     CDS             complement(7025..7351)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211530.1"
                     /locus_tag="TZ53_RS05905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05905"
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="WP_044660686.1"
                     /transl_table=11
                     /translation="MVKVNFRIEEELHERLVRRATAADLSLSEFIRLVLAQAAEPNRGY
                     IFSSKDEILATSIQILSILVVAVGRRSPEAVEQGMAEARALLAERGLLDPELEPNPGAR
                     EAGR"
     misc_feature    7568..7581
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.23"
                     /tool="antismash"
     gene            7812..8156
                     /locus_tag="TZ53_RS05910"
                     /old_locus_tag="TZ53_05910"
     CDS             7812..8156
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250838.1"
                     /locus_tag="TZ53_RS05910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05910"
                     /product="hypothetical protein"
                     /protein_id="WP_044660687.1"
                     /transl_table=11
                     /translation="MPKMTDRERLAKIEADQHSLAQEAESARRVLRAHYGKIAAELAVE
                     RLSEREFREVLSHAIRAGGGAAIAALKALPGASATPEPPPLKKPAKARSKPPEGGGAND
                     DGKALSVVQH"
     misc_feature    7828..7846
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.59"
                     /tool="antismash"
     misc_feature    7849..7862
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 20.71"
                     /tool="antismash"
     gene            complement(8238..9251)
                     /locus_tag="TZ53_RS05915"
                     /old_locus_tag="TZ53_05915"
     CDS             complement(8238..9251)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250839.1"
                     /locus_tag="TZ53_RS05915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05915"
                     /product="DUF2493 domain-containing protein"
                     /protein_id="WP_044660688.1"
                     /transl_table=11
                     /translation="MSKPTASRSVSSFADLPELYAELIATPDFEASFGDPLPLTIMERG
                     EEPAEYDMPEPIAAQAECGGIIASIFDLFTDTRLEALAPEVAWGMVNSLHFVAGKLERR
                     EDQLADNIRDMARRLEPGEVFMKELEDNQLECQSVAEQREAIEAMRDYAAAMYRTCSGR
                     PWSPARGSRASQVTTASQISALDFLRVRAEKRRDRYDPQGPIVVVSGPQKWHDVRIIWN
                     RLDQIRARIPRMMLVTTGQRLGVDVIAAAWAAQRGVHLVTYGIYGRGASAGFKRNREIA
                     ALMPVEAILCEGSGIQQALYELFNPQNGHRVPTHVFLASEQAPDIPIKRRRRVIPA"
     gene            9431..9925
                     /locus_tag="TZ53_RS05920"
                     /old_locus_tag="TZ53_05920"
     CDS             9431..9925
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1197:
                     autoinducer-binding transcriptional regulator,"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /locus_tag="TZ53_RS05920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05920"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_052628117.1"
                     /transl_table=11
                     /translation="MTGFGWGEVPQIIRLSRRQRRILHEARAHGLRDGFTVPVQVAGEY
                     RGTCSFASDRPVELTPHIIGCAQLVGMFCFEVARRITNKDPAPTSKIPELTQRQLDCLV
                     HVGSGKTNWETSVILGVSEDTVDKHVLSAMRRYGVCKRTLLIVRALFDGQLCYRDMLRL
                     H"
     gene            10001..10636
                     /locus_tag="TZ53_RS05925"
                     /old_locus_tag="TZ53_05925"
     CDS             10001..10636
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hserlactone: Autoind_synth"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013362.1"
                     /locus_tag="TZ53_RS05925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05925"
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="WP_044660689.1"
                     /sec_met_domain="Autoind_synth (E-value: 2.4e-22, bitscore:
                     77.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSFQQGVSARVPDALMADMYRDRKRVFIDLLGWDLPALDGRYEI
                     DQFDGPDTIYLIVGLPDGTHLGSMRLLPTHRPTIIGDFFDHLCDAGAPRSRAVWELSRL
                     CLSPRIRAAERRGIRNRLLTSAVQFALHNGISAFCCVTHAWYMPEVMAYGWQCKVLGLP
                     QMDPGGMVGALEIAIDEDTPGLMMQAGTWDETPVQTSFDPSLDRGEVQ"
     gene            10633..11508
                     /locus_tag="TZ53_RS05930"
                     /old_locus_tag="TZ53_05930"
     CDS             10633..11508
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041625.1"
                     /locus_tag="TZ53_RS05930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05930"
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="WP_044660690.1"
                     /sec_met_domain="PhyH (E-value: 8.6e-24, bitscore: 83.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKHQRIGHAADPHLNALRSHGFCLVENIIPPAMVEGLACDLGDSF
                     EATPVSQGPFYGADTVRFGGLLNRSPFAALFVQHPLILDIVENILGPWCDNIQLNLTQA
                     IEIQPGAEQQVPHRDQDMWPVSRMLPSDHEAEYLVNVMWPFTPYRPENGSTILWPGSHR
                     RRDEDLIDPDEGVSLEIAPGSALLFLGSTLHCGGANRSMAARRGMIVSYSLGWLKPYEI
                     QTLVYPPEMAKRFSPDLARLIGYQVHRPNLGNVEGRCPSELLGERQYSGAVDALGDEQI
                     ALIEAFRSQI"
     gene            11614..11889
                     /locus_tag="TZ53_RS05935"
                     /old_locus_tag="TZ53_05935"
     CDS             11614..11889
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1132: DNA-binding
                     protein HU 1"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182407.1"
                     /locus_tag="TZ53_RS05935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05935"
                     /product="HU family DNA-binding protein"
                     /protein_id="WP_044660691.1"
                     /transl_table=11
                     /translation="MNTTELVEAVAAAQGVTKTDAKKILDGFVEAIANAAAKGEEISIN
                     GFGKFKVKASPARDGRNPRTGEPMSIAASRKLGFTPAKAIKDKLNG"
     misc_feature    12063..12077
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     gene            12265..12801
                     /locus_tag="TZ53_RS05940"
                     /old_locus_tag="TZ53_05940"
     CDS             12265..12801
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1126:
                     extracytoplasmic-function sigma-70 factor"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /locus_tag="TZ53_RS05940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05940"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_084694326.1"
                     /transl_table=11
                     /translation="MNDIVPDGRGGRAPVPAASIEALYRHESSRLLRFFSRRTRHEAEA
                     QDLMHDAFLRLSRASIEGDPLPYLLRIASNLLVSSVRRDARWRRLVIDVPIDEGRLLAS
                     PPEQSLNLEAEDVKQQYQRVIDQLPEKTRRIYLMHRLGGRTYIEIAQEMNLSVKAIEYH
                     ISAALKALVRGVDQA"
     gene            12798..13820
                     /locus_tag="TZ53_RS05945"
                     /old_locus_tag="TZ53_05945"
     CDS             12798..13820
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /locus_tag="TZ53_RS05945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05945"
                     /product="FecR domain-containing protein"
                     /protein_id="WP_044660693.1"
                     /transl_table=11
                     /translation="MTDERPDPIARLGNQLYDDAADWFAKMRGPDAKSHEPDFKAWLAR
                     GALHRAAYNKVAEIFTDSGRVDDPDKSRGNEKPRSGPGKRGLAMIALLIGMGAISAKVM
                     FHVGLGRQEAGRLQDASPDQFTIASAVGQIRRVALPDGSSILLDTNSEVQVAFTSKDRR
                     LRLVRGRARFEVAHEQRPFNVAAGAGTVTARGTIFDVRLAESGKVSVALLRGAVDVTVA
                     DSGTTTRQRLRPGEKTIFAPRLETPLPIAAQELNLWTQAKGEFHDEPLIDIVAEANRYA
                     RRPIQIGDVETGRLRVSGIFSLKDTAALSIKLAALFDLEREVSRERIILRRRQQKKIHL
                     "
     gene            complement(13919..14245)
                     /locus_tag="TZ53_RS05950"
                     /old_locus_tag="TZ53_05950"
     CDS             complement(13919..14245)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="TZ53_RS05950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="TZ53_05950"
                     /product="hypothetical protein"
                     /protein_id="WP_044660694.1"
                     /transl_table=11
                     /translation="MAITRAWGVGALVNHRAPLGIFDLKRRFLLQNGKSLRSRHAPLDG
                     RSDQITDLLRIIKDLQAAGLGDGAQGRFHWLRRKIKVGADVLLRRRDDGKCRCIDAHRG
                     EESR"
     misc_feature    complement(14284..14299)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.04"
                     /tool="antismash"
     gene            14311..14619
                     /locus_tag="TZ53_RS05955"
                     /old_locus_tag="TZ53_05955"
     CDS             14311..14619
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="TZ53_RS05955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="TZ53_05955"
                     /product="hypothetical protein"
                     /protein_id="WP_044660695.1"
                     /transl_table=11
                     /translation="MSVSRNAAKISTPVLFNLSDAEYLHSLVSIRALRFFGQPVDAYVF
                     PDEQHIKWQAAHRLAVYERNIAWFDFWLKGIAPRDAETAKRWMMLRDRKSGAENKTG"
     gene            <15103..15885
                     /locus_tag="TZ53_RS05960"
                     /old_locus_tag="TZ53_05960"
                     /pseudo=""
     CDS             <15103..15885
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500735.1"
                     /locus_tag="TZ53_RS05960"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05960"
                     /product="TraB/VirB10 family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="PPPPTAPAAPPAPPQEKVVGSIGSATSASAITVTDGTGAAAPKKK
                     ARTIYLPPGFMKARLLTGIDALASRDATSNPEPLIARVQAPAVLPNDVKANLAGCFVVG
                     NATGSLAKERVEVQLVSLSCVDFDERSVVDQPIKGFFVDTDGKKGLSGKVVTRAGAALA
                     RSFIAGTVSGFSQSVESSFGDISTSALGNVRTLDAGEAAKSGIAGGLGKSSEKLTDFYL
                     DLARQAGPIVEVGAAKDVVLVIQEGVSLEIKPSAGAKF"
     gene            15921..>16382
                     /locus_tag="TZ53_RS05965"
                     /old_locus_tag="TZ53_05965"
                     /pseudo=""
     CDS             15921..>16382
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846823.1"
                     /locus_tag="TZ53_RS05965"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05965"
                     /product="TraV family lipoprotein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPSPGQQRSITHIAWIIPALVTLSGCASIGSLMSPYSEKFSCKNS
                     DHGQCIHPDKAYADAVADRPSRSDPTVTNDKALLKAQGATASPSAASPLARTSKAKAAA
                     ARSAYQGYRDSVYRELQGLIDAPATPMLRPSRAVRTLILPYADRARPDRL"
     gene            16725..18641
                     /locus_tag="TZ53_RS05970"
                     /old_locus_tag="TZ53_05970"
     CDS             16725..18641
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823928.1"
                     /locus_tag="TZ53_RS05970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05970"
                     /product="hypothetical protein"
                     /protein_id="WP_337589245.1"
                     /transl_table=11
                     /translation="MNSTLLLSAAAVAQEAEPNVKDRARSAATASRARTGSSDALQSNY
                     VTPGLSGQTITTVDGKTGFTASLSCQKTSTLLELLVQPGSTGDLTQVRISHDRDFDGTF
                     DSQTLLPVPISGICANGVIACNPGTWEGCRYYGWAVDGALNINLAQTQMTSLSGCYCIN
                     NSCGTNLPWGNISEVLKDLGGGVVGALTTADPRIGVAQAQVEGAMIRYTGAQTTSCTAD
                     PQVSATRYRSNPTDIQGDATSVSQASSVFQALAGSAAGSGKTEQVRSCSIEREVAVKEW
                     RFDDIVAVRGSVSKVTSCGTDCRRYEIRGSGSCDSVPPIYSATFEPLAPARIKSARIVE
                     MGADDWVQARVNGKIVGYAGKRPWLGEALPSGDCRISDDPWYNRTAIDITSQLTSGATV
                     VGARVRGGGGGNWGYVTLEVQVDTGCETTERLVDLCSGYGADPQCRLKSESVDDVVTFN
                     NGVGTGLLPLPQTRTLGTGSCAVQLTRDFFRKDRRYACIIDTGGQATPDLSRAAYIIDR
                     STETVLNDRIQAADGSVQETSRAFSMPTQDSVAACEPICKTRKARTNSDAAPDGVVGEL
                     QTSAQGWDSYYHACSADNVCPAGDGEEIVSACGCLDDFPEAVTMMQTVRLAGADLVCTA
                     GAQ"
     misc_feature    complement(16767..16785)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.87"
                     /tool="antismash"
     gene            18638..19729
                     /gene="traN"
                     /locus_tag="TZ53_RS05975"
                     /old_locus_tag="TZ53_05975"
     CDS             18638..19729
                     /codon_start=1
                     /gene="traN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336429.1"
                     /locus_tag="TZ53_RS05975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05975"
                     /product="conjugal transfer protein TraN"
                     /protein_id="WP_044660697.1"
                     /transl_table=11
                     /translation="MRAPKSLIAWATLLLATCGQPQLAHAQQWLCAADLNGNGDAADDG
                     EIASCTASAGGDHLCPIEQTACTMDTAGSYACPLGSQYACLSADGVSAPSCSPHSCIDT
                     GANPVEEEVLVDDPGTAADGEVDEDGNCLGTIEIFGGRKLRCRPPGLSVTFQNCCKDKD
                     KILHDGMGSSIGSIGTKIAIAKGVFTGMKAAYTAFKAGATASQAASAGANALIVGIDPT
                     SIAISLAINFMMEVLLQGCDQQDMEAGMLRGSGMCHEVGTYCSSKILGICVQKAKSQCC
                     FNSKLGRIIQEQGRPQLKAFANGWGEVKSPNCRGFTAQEFQALDFSKMDLSEYYTEIEA
                     RSADLIQSDMEERVDAYLNAVQN"
     gene            19743..20597
                     /locus_tag="TZ53_RS05980"
                     /old_locus_tag="TZ53_05980"
     CDS             19743..20597
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846833.1"
                     /locus_tag="TZ53_RS05980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TZ53_05980"
                     /product="TrbC family F-type conjugative pilus assembly
                     protein"
                     /protein_id="WP_272947350.1"
                     /transl_table=11
                     /translation="MTLAAGCAITAAQAAPQMTQPTAKLASTAEAEKAMETLADAQARA
                     QRDTKADKPIPSPNIPAPSPALRKRAFDALRSRKSDPRLEERARAARKAGEAQLARDRE
                     AMSKRLAEALGLEAPSTMAAAQAVAPTPATSWVPVLFVSSSMPITTLRTYAGQLERARG
                     VLAFRGMPGGLSKVGPMAKLSAQILRLDPGCEGPACTMRDVQLIVDPILFRQHGVARVP
                     ALGMLPGDPTKPYCEREEEGPASSKSSHIVYGDAALSGMLETYARLGGKEEVRDAQTRL
                     ERR"
ORIGIN
        1 gtgtagattg agatgatcgc atggggtggg gcatgcccca ccccatgcga tttcgatctc
       61 ggtggccacc gggctcatcg gtaaggtgga gttaccacac ttcacacgcc caccgaggag
      121 ttgatcccga tgaccgacga cagactaccg cttgccgagc tgatggcgaa gactggagat
      181 ggagatttcc tgcgcacgat cgccgagagc gtattacaga tcatcatgga ggccgacgtc
      241 gatggcctgg tcggcgctag ccgacacgag cgctccggcg accggacgac ctggcggaac
      301 ggctaccgcg accgcagcct cgatacccgg ctcggcacgc tcaacctgag gattcccaag
      361 ctacggaccg gcgcctactt ccctggcttc ctggaaccca ggaagaccgt cgagaaggcg
      421 ctggtcgcgg tgatccagga ggcgtggatc gccggcgtca gcacccggcg tgtcgacgag
      481 ctggtgcagg ccatgggcat gaccggcatc tccaaatcct cggtgtccaa gctgtgcaag
      541 gatatcgacg aacgtgtcca tgccttcctt aagcggccgc tcaccggcga ctggccctac
      601 ctctggctcg atgctaccta cctcaaggtg cgcgagggcg ggcggatcgt cagtgtcgcc
      661 gcgataatag ccgttgccgt caacaccgag ggcaagcgcg aaatcgttgg ccttcacatt
      721 ggaccctccg aagccgagcc attctggtcg agcttcctca aggacctcgc acgccgcgga
      781 ctgaccggcg tgaagctggt catctccgat gcccacgagg gcctcaaggc agcgatcact
      841 cgcgtgctca gcgccacctg gcagcggtgc cgcgtccact tcatgcgcaa tgccctggcc
      901 tacgtgccca agggccagaa caccgtcgtc gccgctgcca tccgccaggt cttcctccag
      961 cccgaccatg ccgccgcgac acaggtctgg cgccaggtcg ccgaccagtt gcgtacccgc
     1021 tggccaaagc tcggcgcctg catggacgat gccgagcacg acgtgctggc ctacatgacc
     1081 ttccccgagc agcaccgcgt caaattacat tccaccaatc cactggaacg cctgaacaag
     1141 gaagtgaagc gccgcgccga tgtcgtcggc atcttcccga acgaggatag catcatccgc
     1201 ctcgtcggcg ccgtcctgct ggagcagaat gacgaatacc agctccagca ccgctacatg
     1261 cagatcgagg gcatggccgc ccttgctaca ccaatgatcg aggaggcaca gccgctacag
     1321 attacaccca aggccgcctg aaaatgcagc ccgctggcca cacccaattc tacaccacat
     1381 tgacggacgc gaccagagta cacaagccaa agtcacctaa aagcctgtaa atttcactct
     1441 cgctatcggc cgtgccgaac cgttcgaagt gcgaacgcta gcgaggtccg aaaataacca
     1501 aatgtgactt cctgaatttt cgttcccgca atggcgaggc gacgaatgcc tgtcgctgga
     1561 tcaacatgat cgaggcgatg cttcgcgaat atcgcatttg tcgccagcgg gtagggctgt
     1621 gataaatgcc cactgcccgc cagaaatcct ttacgtcgtg gagcgctgag agaaatatcc
     1681 agcgggccgc atcgacattg aagcacggat catcccgcag ccacgacttg atctgcgatt
     1741 cgctccgccc gatcattcgg gcgattcgag gaacccacca actgttgacc tgcatcggcc
     1801 ccaggtcgac ggaaccatcc ctattcctga cttctgcacc aatccatccc ccctcccgct
     1861 tcctcaaggc ccgcagggtt tgggcaagcc atggccggcc ggaagctgcc cgatggatgc
     1921 attgattgat cgccttctcc gccgccagca tgtccaggtc cctcgcctca ccataagctg
     1981 gcgctgcctg aacgatcagc gtggaagcga agccgccggc cagaagcgtt gcacaacacc
     2041 gcccgatcat cggtcgcgcc ctcggtccct gcgcacctcg gtcgtccgat ccggctgttt
     2101 gggctgggag atttccggct gcggctggtt ccgaagcatg cgctcgatga gcgccgtgag
     2161 ggtttccgtt gcgcgaagcc ctgcgccggc cagacccttg ggctcgctcc ctcgattacg
     2221 gtttctgcgt tcggtgcgca gaagggccag ttcacgccgc tgcacgtcat agaggtcgtg
     2281 ttctttctgg atgcgggcgg cttccctttc gagccgtgct tccgttccgc ttcgcttgat
     2341 caggtccagc ccttccaggg acgaggtctt ctggccggaa ttgcgcttca atttctccgc
     2401 gaggtccgcc cggttctcgg tccagagcgt cacgctaaat tcggcacgag tgatcgcggt
     2461 gtagaaattt tgccccgtca ccagcgggga gtcgacggga gcgagaacaa agacccgcgg
     2521 gtaggttttc gactgcgagg agtagaccgt ctccgcatag ccgtgatccc aggtcttgta
     2581 tttcgagaag tcgatctcct gtcgttcgcc cgttcgatcc catcggatcg tggcccgctc
     2641 gttctctatg cgctcgaccg tgccccgctc ggcatttttt agtcccagct ccttcgtcgc
     2701 caggcgccat tggattcgat caccctttgc gaggccccgg tcctgtacgt tgaagacgct
     2761 gaccatggaa gcgcgcgttc ggcgggggtc ccaaaggatg attcggccct gctgatcttc
     2821 cagcctgacg acctcgcgtc cactctcctc tcgcccgaaa ccgacgaccg catattccgc
     2881 ctgccgggcg atccccaact gatcaacatc acggttgaac atgacgacct ggcccttgga
     2941 atagaggcgt gccattcgac gctcctcact cgtcagaccc gctggcgcga ggattttcaa
     3001 cggcacttcc tggatggcga tgacaccttc cttctggagc ccgcgccgga catttgcgtt
     3061 gaccgtgatc cgggtcgcat tgtcgagaac aaggatgttc gtcgcggcgc gcgtttcggg
     3121 ggccagttgc atccattgat cgacgagatt tttcgcaagc ttgctcgctt tgtccccgct
     3181 cacgacgccg tccagaaagc cgatcgcccg cgcatattct ccaaggcgcg ccttcatgac
     3241 cgccttcatc atcttctgat tgttttgctg ccgcagcgat tcggagagct ccgcctttgg
     3301 caatccaagc tgctgaagaa gccagaatgc cttcccttgt tcgatcgcgc ctgtctgctt
     3361 gttgtcgccg aggaagagaa tccgagcgcc ggtatcccga ctgacttcca ggagccgcag
     3421 ggcctgccgg ttactgagct gcccggcttc atcgaccacc agaagatcgc gagcgtccag
     3481 tttccgtccg cccgacacga tcaagttcat gaccgtctgg gagggaattt tcgctttctt
     3541 cccgagttct gctgcggccg atgaggtcgg cgcgagggct tgcacccgga catccgcatc
     3601 caccgttgcg accaacgccc ggaccagggt cgacttaccg gagcccgcca ccccatggat
     3661 tccgaccagg cggtcgcggg acgtggcgag cgtggcgagc gcccgctctt gttgcgagga
     3721 aagcccctcc ccctccagga ccaacagtag gcggtcggtc gatgccaaag gcctagtatc
     3781 gtccaatgcc attgccaggt gtttcgacag agcaatctca agccgggcgg ttgcgcggct
     3841 cgtgcgcccg cgcgtcagga gagcatcgcc ggtctgggtt cgcgtagtca ggagcttttc
     3901 ccgctgttcg tgatccctca caaagggaag gacatcacgg aggctcactt ctcccacatg
     3961 gaccgcgagc cccgttcgga tcagctgacc ctggctattc accgcttcgc gcgtttccat
     4021 gttgcggatg ccgaacagcg cggcgcgccc ggcaatggca cgactgccct catggtcgat
     4081 caccccatcc cggtcggcgg cgcccttcac cttatggagc atttcgagga ggggccccgc
     4141 ctccttgtcc cagcgctcgc gaaggccttc tgtcgtcgtt ttctgcttgg ccttgcgggt
     4201 ctgatagaag gaggcgacgc gagcggcttg ccccttgcga ccatgttcct ccgcgtgtgc
     4261 atcgatctgt tcggcgcgct tggaccaatc tgcgatcagg ctcttgggca cgcccctaat
     4321 ttcgaacagt ccccggtggg gatcagtttc gagttcaaag ccgtgggcat gcagtttctg
     4381 ggcgagccca ttccgataga tttggccagc gaccatctgc tcggcgaaca tcgcccgggt
     4441 ttccatgctg gtcatgggcg cgccgtcctc gcgatcggtg atgttcatca ccacgatatg
     4501 ggtatggagg tgaggatcga gttcccggct ggcatgttcg gtgaagcggg ctgcgactat
     4561 ccgtccggtc gtctcatgga cgatctcccc ctgccaccgg aaacgatagg cggcatgttc
     4621 ctccagccag gagatcgcct ccccgacggc ctgctcgtgc gcttcgagca cgcgttgatc
     4681 ctgcgccacc agggccatga tcgagacgga cttgggggcg ttgaccgcaa agtcccatcc
     4741 cggatgatgc cggatcgtac catcggcgcg ccgccggcca agttgccgtc cctctaccct
     4801 gcccgccagc aactccttga atgtcgtcgg gtcgaccgcc ccctcaaggc ccagttcgac
     4861 ggcgaccttc ccggcccatt cactgtgctc gtcggagccc ttcgtataat agtcgcccac
     4921 cgtataatag cgggcgatgt tgccggccga gcctgacagg cggcggggat ggatcatgcc
     4981 ggatcagcct ctcccgggat ctcgttgacc cggccgtgcc gcaacagcgg aaccgtgcgc
     5041 gcgccagccc gagcgccgag catggatggg ctgggtggtg ttgttttctc ctgcttgcgg
     5101 tctgggggtg aaacctctgg cgtttgtccg aggggcagct cgggcgcaga cttttcggac
     5161 cgaggagcgt tctgcggcgg cggcacccat ccgctcttgc gagcggcttt cttgcgaatg
     5221 cggcccgctg cctgaccgct ttgcgctgcc cgttcttccg ctgcggcgcg ggcggcctgg
     5281 ataatctcat cggccagcgg gagccgaatg ggcggtttgg tgcgttcgac gaatgcgggt
     5341 gcgatgttcg agattgggtt gaaccgatct gcgaaacgga tgacgggaag gtcgcgacca
     5401 aaacgtagaa agccggacag gttggggagg ttcgtgacct ccgtatgcat gaccagcggc
     5461 cgggtcacct gcatccgcga aaggttgacc ccatctcgca tgtcgttgac cccgtaggac
     5521 atgccctcat tcgcttcgat ctgctccacc tgccccagat tttccgagac atgcttcgcc
     5581 gttggcgtgt cgttggcgcg cagggctatc caggttgaac agtagccggt gatcgccgcg
     5641 gcatcctgaa tgccataggt ggcctcaagc tgaggatagg attggaaccc tagtatcccg
     5701 cagccgccat atttgcgcgc gcgggcgagg aagtcgctga gcgacggcaa ccgttgaagc
     5761 gtcggtagct catcgatgat gcaatagagc cgccgctcag ggtcgggctt catgctcatg
     5821 atcgccccga tggcaatatc gatccagacg gtgatgagcg gccgcaagga gggaagttga
     5881 tccgccttca ccgtgatgaa gagccaactg tcatccgtct cattctccac ccaactgcga
     5941 atggaaaagc cgttggcggt gtcgtcgaga taggcaaagc tacgcatgac cgacgcaagc
     6001 tccgcctgga taccggcgga ggtccggtct ccttccgtgc tgatgaaggc tgcggcctcg
     6061 gtcccctcgg cgaaagcgac gaggtccttc agccccgacc gaagaaggcg atcgagaaga
     6121 atggagacca gcgtgtgttt ctgcctagcg agcttgcgca ggacgccgac cagcgtcccc
     6181 cgcgctgcct tcgcccagaa aggatcgccc gccttgtcgg ggatcgtgga ctgggcgatc
     6241 tggtcataat gatattcccg agggacatcg acccagggcg accaatggtc ggttcgctgg
     6301 tcgagaggat tgaggagaat gtccttcccc ggccggtaga atttttcaac gaaggagcca
     6361 gcggtatcat agacgatcgc gcgctttccg tttttcctga taccctccag catcgtcgtg
     6421 atgatgttgg tcttgcccgt gccgggcgcg ccgcacagga caatatgctc cggctcgaag
     6481 gcgtgcggca tcgccacgcc gccgatggtg aaactgccct tgcggtatcg gcgcaggagc
     6541 cgccggatcg atctgagggt cccgaatcgg gcgccccgga ggaactggtt cgaccccaac
     6601 ccccgtccgg tccgcgtgaa ataataccag gcccaaccga gagcgagcgc ggccccgatg
     6661 cctccgatga gggcgccggt gatgagcgct gcttcgcccg cctccagcgt ttttctcgca
     6721 accccggagt tcagtaaggc atccgccgtc gtccaatatt tgtgaccgcc gggcgtctga
     6781 aactggaccg gatcggcggc gccccgtgtc gcatccttcc ggaattccgc aagggccagc
     6841 tggaccagca catagcgctg ataggcggtc agggtctcga agcagtacca gaggatgccc
     6901 atgacccaga gcaccagaca ggccgacatg gtctggaaaa agacctgggt cgtcattcgg
     6961 acgttgtgga cgatagcctg gccgccacgg gtccaggagc cgagcgtatc gttgcggaag
     7021 atactcatcg tcccgcctcc cgggcacccg gatttggttc cagttccgga tcgagcagcc
     7081 ccctctcagc gagcagggcg cgggcttcgg ccatcccctg ttcgaccgct tccggcgatc
     7141 gccgccctac cgcgacgacg aggatggaaa gaatctgaat actggtggca aggatctcat
     7201 ccttggacga gaagatatag ccgcgattgg gttcggcggc ctgcgctaga accagcctga
     7261 taaactccga caggctgagg tcggcggccg tggcgcgccg aacgaggcgc tcatggagct
     7321 cctcttcgat cctaaaatta actttgacca cagggatgct ccaatagctg gagccctgcg
     7381 gatgtcgtga agcgccgccc ttataccctt gattgcgctc gggttcaaat cgatcgcgtg
     7441 taatacatgt attccgccca aaaaggccgg attacggcgg gtttcgaggc ctgccattcc
     7501 cgcttgtggt acatggacca caaaatgtgg tttcgggcga ttatccgtat tcgaccatat
     7561 atcattgatt ttatgatata ttcccgcgca gcacccgtgc gtagggtgca ttacatcggc
     7621 cttggggccg tgcgtccgcc cttcgcagcg cctggccgct gcgtccgtta tcagacggcg
     7681 cgggcgggcc tcctcgaacg gagtttcctg cggtcgtcaa agcgggcgat ctgggatgag
     7741 cgttgcaagc gtctgctcct tggggaatag tcggggacgg cgggcagacc cttgcaatga
     7801 ggagtcgata catgcccaag atgacggatc gcgagcggct cgcgaagata gaggccgatc
     7861 aacatagcct tgcccaagag gcggaatcgg cgcggcgcgt cttacgtgcg cactatggaa
     7921 aaatcgctgc ggagcttgcc gtcgaaaggc tgagcgagcg cgaatttcgg gaggtgctta
     7981 gccacgccat ccgggccggt ggcggtgctg caatcgccgc gctcaaggct ctgcccggtg
     8041 ccagcgcgac acctgaaccg ccgccactca agaagcctgc gaaggcgcga agcaagccgc
     8101 cggaaggcgg cggagcgaat gacgacggca aggcgctttc ggtggtccag cactagcgat
     8161 ataggatcgt gccgcaggca cgaaaaaagg accggggcgc atttgcgggc cggtcccttc
     8221 cttttctgcc ttggtgtcta ggcgggtatc acgcgccgcc tgcgcttgat gggaatgtcg
     8281 ggtgcctgct cactcgccaa aaagacatgc gtcggcacgc gatgcccgtt ctgcgggttg
     8341 aacaattcat agagggcctg ctgaatcccc gatccctcgc acaagattgc ctcaaccggc
     8401 atcagcgctg cgatctcgcg gttgcgcttg aatcctgcgc tcgctccgcg tccatagatt
     8461 ccgtaggtca caagatgcac gcccctttgc gcggcccatg cggcggcgat gacgtccacg
     8521 cctagccgct gccctgtcgt caccaacatc atgcgcggaa tgcgcgccct gatctggtcc
     8581 agcctgttcc agataatgcg gacgtcgtgc cacttctgcg ggccggaaac gacgacgatg
     8641 gggccttgcg ggtcatagcg gtcccgcctc ttctctgccc ggacgcgcag gaagtcgagc
     8701 gcagaaatct ggcttgcggt ggtcacttgg gaggcccgcg atccccgcgc cggggaccat
     8761 gggcgacctg agcaggtgcg atacatggcg gcggcataat ccctcatcgc ctcaatggct
     8821 tcgcgttgtt cggccactga ctggcattca agctggttgt cctccagttc cttcatgaag
     8881 acttcgcccg gctccagccg ccgcgccata tcgcggatat tgtcggcaag ctgatcctcc
     8941 cgccgctcca gcttccccgc aacaaagtgc agcgaattga ccatgcccca tgcgacttcg
     9001 ggcgccagcg cttcaaggcg ggtgtcagtg aagaggtcga aaatggacgc gatgatgccg
     9061 ccgcattccg cctgcgcggc gatgggttcg ggcatgtcat attcggcggg ttcctcgccg
     9121 cgttccatga tcgtaagcgg gagaggatcg ccgaacgatg cctcaaaatc gggcgtggcg
     9181 atgagttcgg catagagttc cggcaggtcg gcaaagctgc tcacgctccg ggaggcggtt
     9241 ggtttgctca tggggttgct ccttcctgag aggatgacca ttccgggccg ggagagcgtg
     9301 acggcgcttc ccggcccgtc ttgtctgccg ttagaaaggc acctcatcgt caaggtcgtt
     9361 ccgcccgccg ccggtaatcg cgaaccgtta ttttcaggac gatcccatcc tggctgccag
     9421 caatcgccga ttgacaggtt ttggctgggg cgaggtaccc cagatcattc gcctgagccg
     9481 tcgccagcgg cggatcttgc acgaggcgcg cgcccatgga ttgcgtgatg gatttactgt
     9541 cccggttcag gtggcgggcg agtatcgggg tacatgctcc tttgcaagtg atcggccggt
     9601 tgagctgacg ccccacatta tcgggtgcgc ccagctggtg ggaatgttct gcttcgaggt
     9661 ggcgcgcagg attacaaata aggatcctgc cccgacttcg aaaattcctg aactcaccca
     9721 gcggcaactg gattgcctgg tgcatgtcgg ctcaggcaag accaattggg aaacgagcgt
     9781 gatactgggc gtgtcagaag acaccgtaga caagcatgtc ctgagcgcca tgcggcgcta
     9841 cggcgtatgc aagaggactt tgttgatcgt ccgcgcgtta ttcgacggac aactctgcta
     9901 ccgagacatg ttgcgattgc actaaatcac cccctttcgg ggaatagcgc ggaatcgcca
     9961 tccttcgcat cctgcctgtc tcacacacag gaggaacgtg atgctgtcat ttcaacaggg
    10021 tgtatctgcg cgcgtgcccg acgcgctcat ggcggacatg tatcgcgacc gaaagcgggt
    10081 ctttatcgac ttgctggggt gggatttacc agccctggac ggcaggtatg agatcgacca
    10141 gtttgacggt cccgatacaa tctacctcat cgtcgggctc ccggatggca cccatctggg
    10201 atcgatgcgc ctgttgccca cccatcggcc cacgatcatc ggcgatttct tcgaccatct
    10261 ctgcgatgcc ggagcaccca gatcgcgagc ggtctgggaa ctttcccgat tgtgtctcag
    10321 tccgcgcatc cgcgcggcgg aacgtcgcgg catccgcaat cgcctgctga catcggctgt
    10381 ccagttcgcg ctgcataacg ggatcagtgc cttctgctgc gtgacccatg cctggtatat
    10441 gcctgaggtc atggcctatg gctggcaatg caaggtgctg ggtcttccgc agatggaccc
    10501 gggcggcatg gtcggcgcgc tggagatcgc gatcgatgaa gacacgccgg gcctcatgat
    10561 gcaggcgggg acctgggacg aaacacccgt tcagacctcg ttcgacccct cgcttgatcg
    10621 gggagaggtc cagtgaagca ccaacgcatt ggccatgcgg ccgaccctca cctcaatgct
    10681 ctgaggagcc acgggttctg cctcgtcgag aacataattc cgccggcgat ggtggaaggc
    10741 ctcgcttgcg accttggcga cagctttgaa gcgaccccgg tgtcccaagg ccccttctat
    10801 ggggccgaca ccgtgcggtt tggcggtctg ctgaaccgat cgccctttgc cgcccttttc
    10861 gtccagcatc cgctcattct cgacatcgtc gaaaacatcc tgggcccatg gtgcgacaat
    10921 atccagctca accttacaca ggcgatcgaa atccaaccgg gcgccgagca gcaagtgccc
    10981 caccgcgatc aggatatgtg gccggtcagc cgcatgctcc ccagcgacca tgaggcggag
    11041 tatctggtga atgtgatgtg gccattcacg ccctatcgac cagagaacgg ttcgaccatc
    11101 ctatggcccg gcagccatcg tcggcgcgac gaagacctga tcgatccaga cgagggcgtg
    11161 tcgctggaga tcgctccagg gtccgcgctt ctgtttctgg gatcgaccct gcattgtggc
    11221 ggcgcgaacc ggagcatggc tgcgcgccga ggtatgatcg tcagctattc gcttggctgg
    11281 ctcaaaccct atgaaatcca gacgctcgtt tacccgcccg aaatggcaaa gcgattttca
    11341 ccggatctgg cgcgcctgat cggctatcag gttcatcggc ccaatctcgg caatgtggaa
    11401 ggacggtgcc cgtcggagct gcttggcgag cgccaatata gtggcgctgt ggacgccctg
    11461 ggagacgaac agattgcctt gatcgaggca ttccgaagcc agatttgatg gtgaaacagg
    11521 gatacgcccg ccagtacggc acgcactttc ccatcgaccg gctggcggct tctcggcatg
    11581 agcgacgctt cctacaagag ggaaacttag ctgatgaata ccactgagct ggtcgaggcg
    11641 gtcgccgccg cccagggcgt caccaagacc gacgcgaaga aaatcctgga cggctttgtt
    11701 gaagcgatcg ccaatgccgc cgccaagggc gaagaaatct cgatcaacgg ctttggcaag
    11761 ttcaaggtca aggcgagccc ggcacgcgac ggccgcaatc cgcggactgg cgaaccgatg
    11821 agcattgctg catcgcgtaa gctcggtttc acgcctgcca aggcgatcaa ggacaagctc
    11881 aacggctgat ccaaaaagct ccccgggccg atttattcgg cctggggagc gaaaatgcat
    11941 cgtcatggcc aacgacagga gaccctgccc ttccccttga cagggcggtg ccgctatgca
    12001 gacttgaagg aagcgagaca ggactgccgg cgacctctcg cagattttct cgtcaggcat
    12061 caatcacgct cgtccatgac ggccgagcgg ccgtaccgga tcaaagcccg gcgctgatgg
    12121 cgcagagcga acggcggcga cagcgtgagc ggattggtcc ggttttccgg atggttcgtg
    12181 tcggcatgta gcgttatccc atgttcatct cttgcctttg ctcatccttg ggcagcgacg
    12241 cagtgcctgg aaggtatcca ggcaatgaat gacattgttc cagacggtcg tggaggccgt
    12301 gcgcccgtgc cagcggcctc gatcgaggcg ctctatcgac atgaatccag ccgcctcctc
    12361 cgctttttct cacgtcgaac gcggcacgaa gccgaggcac aggatctcat gcatgacgca
    12421 tttctccgtt tgtcccgcgc cagtatcgag ggagatcccc tgccctattt gctcagaata
    12481 gccagcaatt tgctcgtcag cagcgttcgg cgcgatgcca gatggaggcg ccttgtcatc
    12541 gacgtgccga tcgatgaagg caggctgctt gcgtcgccac cagagcagtc attgaacctt
    12601 gaggccgaag acgtgaagca acaatatcaa agggttattg accaactacc cgagaaaacc
    12661 agacgcattt atctgatgca tcgcttgggc gggcgaacct atatcgagat agcgcaggag
    12721 atgaatctga gcgtcaaggc gatcgagtat cacatatcag cggcgttgaa ggcgcttgtg
    12781 agaggagtgg atcaggcatg actgacgaga gaccggatcc catcgcacgc ttgggcaatc
    12841 agctctacga cgacgcggca gattggttcg ccaaaatgcg cggtcccgac gccaaatcgc
    12901 atgagccgga tttcaaagcc tggcttgccc gcggtgcact gcaccgggcg gcctataaca
    12961 aagtggcaga gatattcacc gacagtggac gagtggatga tcctgataaa agccgtggca
    13021 acgaaaagcc gcgatccggg ccaggcaagc gcggcctggc aatgatcgcc cttcttattg
    13081 gcatgggtgc aatctcggcg aaggtgatgt ttcatgtcgg ccttggtcgc caggaggcag
    13141 ggcggctgca ggacgcgtca ccagatcaat tcaccattgc gagcgccgtc ggccagatac
    13201 ggcgcgtcgc gctaccggat ggatcgtcca tccttctcga tacgaacagc gaggtgcagg
    13261 tcgcgtttac gtcaaaagac cgacgcctca gacttgtccg agggcgtgcc cggttcgagg
    13321 tcgctcatga gcagcgtcca ttcaatgtag ccgccggcgc ggggacggta accgcgcgcg
    13381 gtacgatatt tgacgtgcgt ctcgccgaaa gcgggaaagt gtcggtcgct ctgctccggg
    13441 gcgcggtcga tgtcactgtg gccgactctg gaaccaccac tcgccaacgg ttgcgcccag
    13501 gggaaaagac gatcttcgct cctcgcctgg aaacccctct gcccattgcc gcccaagaac
    13561 taaatttatg gacgcaggca aaaggcgaat ttcatgacga gccgctgatc gatatcgtgg
    13621 ccgaggccaa tcgctacgcc cgtcggccca tccagatcgg cgacgtcgag acagggcggc
    13681 tgcgggtgtc tggcatattc tcgctgaagg acacggcggc gttgagcatc aagcttgccg
    13741 ctctttttga cctagagcga gaggtaagca gggaacggat catcttgcgg cggcggcagc
    13801 agaaaaaaat tcacctatga tttagggaga cacctcagcg aggcgtgaaa gccccatgag
    13861 cccggcaaaa tgaacaagcc gggccgaagg gggctttatg agtattaccc gcaaattgct
    13921 accgactctc ctcgcctcgg tgtgcatcaa tgcagcggca tttcccatca tcgcgtcggc
    13981 gcagcaggac gtcggcgccg actttgatct tccgtcgcag ccaatggaaa cgaccctgcg
    14041 cgccatcgcc caggccagca gcctggaggt cctttatgat gcgcaggaga tcggtgattt
    14101 gatcgctcct gccatcaagg ggcgcatgac gggatcgcag gctcttgccg ttctggagca
    14161 gaaatctccg cttcagatcg aatatgccca ggggagcgcg gtggttcaca agcgcgccca
    14221 cgccccaggc gcgggtgata gccattcaga ggccatcatc gtcactggta cccgcgtctg
    14281 tcggacaatg atcagccttt ctgggccaat atgtcggttt cccgcaatgc agcgaagatt
    14341 tccacgcctg ttttgttcaa tctttccgac gccgaatatc tccactcgct ggtgtcgatc
    14401 agggcattgc gttttttcgg gcaaccggtc gacgcctatg tcttcccgga cgagcagcat
    14461 atcaaatggc aggctgccca ccgcctcgcc gtctatgaac gcaatatcgc ctggttcgac
    14521 ttctggctga agggcatcgc gccgcgtgac gcggagacgg caaagcgatg gatgatgctg
    14581 cgcgacagga agtccggagc cgagaacaag acgggctgac ctggacataa ggatctaacc
    14641 aaggctcgac caatgtcgac accaggcttc ggtgtcggcg agttgaagca aggcggggcg
    14701 cttcccccaa atgtgggagt tcgcgatgat tattgccttt acttgtctgg cgtcagccag
    14761 ccttgcagtg caagcggcga caccatcccg gccagggcgg gaaattgaag atggatttcc
    14821 aaaatccctc agcgcgccgc accagcatat cactgccgaa ggcggtgact ttgtgatccg
    14881 agagaggtca cgccctgcta acgtgcaaca gcccgagcgg cggactttca ggctcggcgc
    14941 ccatgtttcg ctgtcgcgcc tcaccggctc gtccgcatcg aggaaggata cggcccatga
    15001 agcgcgctaa gcccctgttc ggcctgatcc tgcttgggtc gcttgcgata tccgccgacc
    15061 ggcggacagg gcgcgcaatc gggacccgag cagaacgaca ttccgccccc gcccacggcg
    15121 cccgccgcgc cgcctgcccc accgcaggag aaggtggtgg gctcgatcgg cagcgcgacc
    15181 agcgctagcg ccataacggt gaccgatggg acaggagcag ccgcacccaa aaaaaaagcc
    15241 cgcacgatct atttgccgcc tggtttcatg aaggcgcggc ttctgaccgg gatcgacgcc
    15301 ttggcgagcc gggatgcgac cagcaatccc gaaccgctga tcgcgagggt tcaggcgcct
    15361 gcggtcctgc ccaatgacgt caaggcgaac cttgccggct gcttcgtcgt cggcaacgcc
    15421 accggcagcc tcgccaagga acgggtcgag gtgcagctgg tctcgctctc ctgcgtcgat
    15481 tttgacgaac gatcggtggt cgatcagccc atcaagggct tcttcgtcga taccgatggg
    15541 aagaaaggcc tctcgggcaa ggtcgttacc agggccggcg ccgcgctcgc ccgttccttc
    15601 atcgctggca ccgtcagcgg cttctcgcaa tcggtcgaat ccagcttcgg cgatatttcg
    15661 acctcggcgc tgggcaatgt ccgcacgctc gatgcgggcg aggccgccaa gtccgggatc
    15721 gccggcgggc tcggcaagtc ctccgagaag ctcaccgact tctatctcga tctcgcgcgg
    15781 caggccggcc cgatcgtcga ggtcggcgcc gccaaggacg tcgtcttggt catccaggaa
    15841 ggcgtctccc tcgaaatcaa gccaagcgcg ggagcgaaat tctgatgcgc tgcgcgacac
    15901 cgatacagga gtcaaagccc atgccgtctc caggccagca gcgatccata acccatatag
    15961 catggatcat cccagccctc gtaacgctgt ccggctgcgc gagcatcgga tcgctgatgt
    16021 cgccctattc ggagaaattc tcctgcaaga atagcgacca tggccagtgc atccatcccg
    16081 acaaggccta tgccgatgcg gtggcggatc gcccgtcgcg ctcggacccc acggtcacaa
    16141 acgacaaggc gctgctgaag gcgcagggcg cgacggcatc accatccgca gcgagcccac
    16201 tagcgcgcac cagcaaggcg aaggcggccg cagcccgaag cgcgtatcaa ggctatcgcg
    16261 acagcgtcta tcgcgaattg cagggcctga tcgatgcgcc ggcgacgcca atgcttcggc
    16321 cttctcgcgc cgttcgcacc ctcatccttc cctatgccga ccgggcgcgg cccgatcgcc
    16381 tctaggggcg ccatgagcag atcacccacc ccatgcaccg tgcgcggccc aaaggccgcc
    16441 tcgtctgccc gccacagccc ccggggccgg ctggacgcgc gcgatgccgg agcggcgtcg
    16501 ccccccttag ctggcgccac tccaaacccg gtcagcgcga ccgtaacccc catgaagcag
    16561 cgtgccccct ctcctcgccc gccgcttgac gccgcgctga ccatctttga aggccaggcc
    16621 gtatttcgcc tgtcgaccgg agaacttatc aggctacgga ccggaagccg cactgtctgg
    16681 ggcgatcggt catgactgtc cggcgccatc tgctctttgc agtcttgaac tccacacttc
    16741 tcctgtcggc cgctgccgtc gcgcaggaag ccgagcccaa cgtcaaggac cgcgcccgca
    16801 gcgcggcgac cgcctcgcgc gcccgcaccg gctccagcga tgccctgcag tccaactatg
    16861 tgaccccggg cctgagcggc cagaccatca ccacggtcga tgggaagacc gggttcaccg
    16921 cgtccttaag ctgccagaag acatcgacct tgcttgaact gctggtgcag ccgggaagca
    16981 ctggcgacct cacacaggtc cgcatctccc atgatcgcga ttttgacggc acgttcgaca
    17041 gccagaccct gctcccggtg ccgatctcgg gcatctgcgc caatggcgtc atcgcctgca
    17101 atcccggcac ctgggaaggc tgccgctatt atggctgggc agtcgatggc gcgctcaata
    17161 tcaatctcgc gcaaacccag atgacgagcc tgtcgggctg ctactgcatc aacaacagtt
    17221 gcgggacgaa cctgccctgg ggcaacatct cggaggttct gaaagatctg ggcggcggcg
    17281 tggtcggcgc cctcacgacc gccgatccgc gcatcggtgt cgcgcaggcg caggttgagg
    17341 gggccatgat ccgctatacc ggggcgcaaa ccacatcctg cactgctgat ccgcaagttt
    17401 cagctacgcg ctatcgcagc aacccgaccg acattcaggg agacgccact tccgtctcgc
    17461 aggcaagcag tgtctttcag gctctggcgg ggtctgccgc aggcagcggc aaaaccgagc
    17521 aggtccgcag ttgttcgatc gagcgcgagg tcgccgtcaa ggaatggcgt ttcgacgaca
    17581 tagtagccgt gcgcgggtcg gtcagcaagg tcacgagctg cggcactgac tgccggcgct
    17641 atgagatccg cggctcgggc agctgcgaca gcgtaccgcc catctacagc gcaacattcg
    17701 agccactggc gcctgctcgg ataaaatccg ctcgcatcgt cgaaatgggc gccgacgact
    17761 gggtccaggc cagggtcaac ggcaagattg tcggctatgc gggaaagcgc ccatggctcg
    17821 gcgaagcatt gccatcgggc gattgtcgca tcagcgatga cccctggtac aacaggactg
    17881 ccatcgacat cacatcccag ctgaccagcg gcgcgacggt cgtcggcgcg cgcgtgcgag
    17941 gcggcggtgg tgggaattgg ggctatgtca cgctcgaagt gcaggtggac acaggctgcg
    18001 agacgaccga gcgactggtc gatctatgct caggctacgg cgcggacccg caatgccgcc
    18061 tcaaaagcga gagtgtcgat gacgttgtga cgttcaacaa cggggtcggc actggcctct
    18121 tgccactgcc ccagacgcgg acgttgggca cgggcagctg cgctgtccaa ttgacccgcg
    18181 atttcttccg gaaggatagg cgctacgcct gcatcatcga cacaggcggg caggctacgc
    18241 ccgacctctc ccgcgccgcc tatatcatcg accgctcgac cgagacggtg ctcaacgacc
    18301 ggattcaagc ggccgatggc agcgtccagg agacctcccg cgccttctcg atgccgacgc
    18361 aggacagcgt cgcggcttgc gaaccgatct gcaagacgcg gaaagccagg accaacagcg
    18421 atgccgctcc cgatggcgtg gtgggcgaac tccagacctc ggcccagggc tgggacagct
    18481 attatcatgc ctgctcggcc gacaatgtct gtccggcagg cgatggcgag gagatcgtct
    18541 cggcctgcgg ctgccttgac gattttcccg aagccgtgac catgatgcaa acggtgcggc
    18601 tcgcaggcgc ggacctggtt tgcacggcag gtgcgcaatg agggcgccca aatctcttat
    18661 cgcctgggcg acgctgctgc tggcgacctg cggccagccc cagctcgccc atgcgcagca
    18721 atggctttgc gccgccgatc tcaacggcaa tggcgatgcg gccgacgacg gcgaaatcgc
    18781 tagctgcacg gcgagcgcag gcggcgatca cctctgcccg atcgagcaga cggcctgcac
    18841 gatggacacc gccggcagct atgcctgtcc gcttgggagc caatatgcct gcctgtcggc
    18901 cgacggcgtc tctgcgccga gctgctcgcc ccatagctgc atcgacaccg gcgccaaccc
    18961 ggttgaagag gaggtgctgg tcgatgatcc tggcaccgcc gcagatggcg aggtcgacga
    19021 ggacggcaat tgccttggca cgatcgaaat tttcggcggg cgcaagctac gctgccgccc
    19081 gccgggcctc agcgtcacat tccagaattg ctgcaaagac aaggacaaga tcttgcatga
    19141 tgggatggga tcgtcgatcg gttcgatcgg caccaagatc gccatcgcca agggcgtatt
    19201 caccggcatg aaggccgcct acaccgcctt caaggccggc gcgacggcaa gccaggcggc
    19261 gagcgcgggc gccaatgcgc tgattgtcgg catcgatccg acctcgatcg ccatcagcct
    19321 agccatcaat ttcatgatgg aggtgctgct ccagggctgt gatcaacagg atatggaggc
    19381 cgggatgctg cgcggctcgg gcatgtgcca tgaggtcggc acctattgct cgtccaaaat
    19441 cctgggcatc tgcgtccaga aggcaaagag ccagtgctgc ttcaacagca agctgggccg
    19501 catcatccag gaacagggcc gaccgcaatt gaaggcgttc gccaatggct ggggcgaggt
    19561 caagtcgccc aactgtcgcg gcttcactgc gcaggagttc caggccctcg acttcagcaa
    19621 gatggacctc agcgaatatt acaccgagat cgaggcccga tcggccgatc tcatccaatc
    19681 cgacatggag gagcgcgtcg atgcctatct caacgccgtc cagaattagg ctgatcacat
    19741 ccgtgaccct ggctgccggc tgcgcgatca cggcagcgca ggccgcgccg caaatgacgc
    19801 agcccacggc gaaattggct tctaccgccg aggctgagaa ggccatggaa accttggcgg
    19861 acgcccaagc acgggcgcaa agggacacca aggcagacaa gccgatcccc tcccccaaca
    19921 tccccgcgcc ttccccggca ttgcgcaaac gcgcgttcga cgcgcttcgc tcgcgtaaat
    19981 ccgatccacg ccttgaggaa cgggcgcgcg ccgcgcgaaa agcgggcgaa gcccagcttg
    20041 cgcgggatcg cgaggcgatg agcaagcggc tggctgaggc gctgggcctc gaggcgccat
    20101 cgacgatggc tgcggcgcaa gcggttgccc cgacgcctgc tacgtcctgg gtgccggttc
    20161 tcttcgtgtc atcgtcaatg ccaataacca ccttgcgtac ctatgccggc cagctcgaac
    20221 gcgcgcgggg cgtgctggca ttccgcggta tgcccggtgg cctcagcaaa gtcggcccca
    20281 tggcgaagct ctcggcgcag atcctccggc tcgatccggg ctgcgaagga ccggcctgca
    20341 caatgcgcga tgtccaactc atcgtcgatc cgatcttgtt ccgccagcat ggcgtggccc
    20401 gtgtgcctgc tctcggaatg ctgcccggtg atccgactaa gccctattgc gagcgagagg
    20461 aggaagggcc cgcatcgtcg aagtccagcc atatcgtcta tggcgacgcg gcactctccg
    20521 ggatgctcga aacctatgcc cgccttggcg gaaaagagga ggtgcgcgat gctcagactc
    20581 gcctggaacg ccggtaaatt catctggcca agtgtccgcg atcttccccg gcacgc
//
