LOCUS       NZ_BBNO01000011        10417 bp    DNA     linear   CON 19-AUG-2024
DEFINITION  Streptomyces lydicamycinicus strain TP-A0598, whole genome shotgun
            sequence.
ACCESSION   NZ_BBNO01000011
VERSION     NZ_BBNO01000011.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces lydicamycinicus
  ORGANISM  Streptomyces lydicamycinicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Komaki,H., Hosoyama,A., Igarashi,Y. and Tamura,T.
  TITLE     Streptomyces lydicamycinicus sp. nov. and Its Secondary Metabolite
            Biosynthetic Gene Clusters for Polyketide and Nonribosomal Peptide
            Compounds
  JOURNAL   Microorganisms 8 (3), 370 (2020)
   PUBMED   32155704
  REMARK    DOI:10.3390/microorganisms8030370 Publication Status: Online-Only
REFERENCE   2
  AUTHORS   Komaki,H., Ichikawa,N., Hosoyama,A., Fujita,N. and Igarashi,Y.
  TITLE     Draft genome sequence of marine-derived Streptomyces sp. TP-A0598, a
            producer of anti-MRSA antibiotic lydicamycins
  JOURNAL   Stand Genomic Sci 10, 58 (2015)
   PUBMED   26380643
  REMARK    DOI:10.1186/s40793-015-0046-5 Publication Status: Online-Only
REFERENCE   3  (bases 1 to 214881)
  AUTHORS   Hosoyama,A., Kimura,A., Ohji,S., Ichikawa,N., Yamazoe,A. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2014) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Newbler v. 2.6
            Genome Coverage                   :: 93x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina HiSeq 1000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000829715.2-RS_2024_08_19
            Annotation Date                   :: 08/19/2024 02:08:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,323
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,092
            CDSs (with protein)               :: 7,092
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 4, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 54 of 145
            Pseudo Genes (incomplete)         :: 123 of 145
            Pseudo Genes (internal stop)      :: 9 of 145
            Pseudo Genes (multiple problems)  :: 39 of 145
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:07:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 78334
            Orig. end                         :: 88751
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBNO01000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5417
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(15..980)
                     /locus_tag="TU31_RS33230"
                     /old_locus_tag="TPA0598_11_00770"
     CDS             complement(15..980)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536882.1"
                     /locus_tag="TU31_RS33230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_11_00770"
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_042161825.1"
                     /transl_table=11
                     /translation="MPRRLAATLVATALTAGPAVLLGAVPAHATGDHGTARGTADAVVL
                     RTGVNVGLLNKTVDVPLNVALNEVHAPASANKTALTVKLDGIDHGRPFSVLRADAATAR
                     ATADRRAAEGYANLVRAKVHLPGLPLLSVIEVQQVTAKAVCAAGARPRAESNVLGTVRV
                     LGQKVTLTAGGTTKVKVPGVGQVRLDLSKKNVTSKGAAATALDLDVSVNPLKLNVADVH
                     GRVTLAKATCTTPRGKAPEDNGSGGTGGGQTGGGTEPAGGKGTEVSRTGSGPSTQNLAE
                     TGGSSATPYLAGGAGLLLAAGAGAMVFARRRRSAAPEGRG"
     gene            complement(1268..2359)
                     /locus_tag="TU31_RS33235"
                     /old_locus_tag="TPA0598_11_00780"
     CDS             complement(1268..2359)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606343.1"
                     /locus_tag="TU31_RS33235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_11_00780"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_042161631.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 3.6e-18, bitscore:
                     59.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEGAVLHLKGRVLVGPDDVRDELWVVGGRVTFDRPVMARDATTV
                     TGWALPGLVDAHCHVGLDAHGPVDAPTSEKQALTDRDAGTLLIRDAGSPSDTRWVDDRE
                     DLPRIIRAGRHIARTKRYIRNYAHEIEPEDLVAYVAQEARRGDGWVKLVGDWIDRSTGD
                     LAACWPRGAVEAAIAEAHRLGARVTAHCFAEDTLAPLVEAGIDCIEHATGLTEETIPLF
                     AERGVAIVPTLVNIATFPRLADGGAAKFPRWADHMRRLHDRRYDTVRAAYDAGVPVFAG
                     TDAGGSLAHGLVAEEVVELTRAGLPAVAALSATTWGARDWLGRPGLTEGAPADLVVYES
                     DPRADVRVLAAPRRVVLRGRIVG"
     misc_feature    2555..2568
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.64"
                     /tool="antismash"
     misc_feature    complement(2790..2805)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(2800..2815)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     gene            2971..3540
                     /gene="ectA"
                     /locus_tag="TU31_RS33240"
                     /old_locus_tag="TPA0598_11_00790"
     CDS             2971..3540
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536877.1"
                     /locus_tag="TU31_RS33240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_11_00790"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_042161633.1"
                     /transl_table=11
                     /translation="MTAAQADHAGARSDIREFPEGFKLDTPRVEDGAAIWRIARDSKAL
                     DLNSSYSYLLWCRDFAATSVVARDAEGEPAAFITGYLRPERPETLVVWQVAVDDAHRGQ
                     GLAAALLDGLAARARDELGVRFVETTVTPDNTASNRLFASFAERHSVPLKSEVLFDAGL
                     FPEQGHEPEVLHLIGPFEPPARTGHS"
     gene            3677..4945
                     /gene="ectB"
                     /locus_tag="TU31_RS33245"
                     /old_locus_tag="TPA0598_11_00800"
     CDS             3677..4945
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 7.2e-75.
                     Score: 244.0. Matches aSDomain:
                     nrpspksdomains_TU31_RS33245_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="TU31_RS33245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_11_00800"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_042161636.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.2e-78, bitscore:
                     257.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRSWPTVFDRAQGSRMTDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGANSVEAALKLARKVKGRESIVSFTNAFHGMSLGALAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYLDGTYPDFLWFERLLEDQGSGLNRPAAVIVETVQGEGGINVARA
                     EWLRALSELCERWDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAATLDTYWADGQMEKQTLARGEIVEAHLK
                     AIAEEHPDTIVEYRGRGLVWGLECTPKPLADKIAARAFELGLLIETSGPESEVVKLLPA
                     LTTTPEELDEGLRILARAVRDCA"
     aSDomain        3761..4771
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TU31_RS33245_Aminotran_3.1"
                     /evalue="7.20E-75"
                     /label="TU31_RS33245_Aminotran_3.1"
                     /locus_tag="TU31_RS33245"
                     /protein_end="365"
                     /protein_start="28"
                     /score="244.0"
                     /tool="antismash"
                     /translation="FDRAQGSRMTDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGANSVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGALAVTGNSMKRAGAGIPLVHGTPMPFDNYLDGTYPDFLWFER
                     LLEDQGSGLNRPAAVIVETVQGEGGINVARAEWLRALSELCERWDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAATLDTYWADGQMEKQTLARGEIVEAHLKAIAEEHPDTIVEYRGRGLVWGLECT"
     gene            5001..5417
                     /locus_tag="TU31_RS33250"
                     /old_locus_tag="TPA0598_11_00810"
     CDS             5001..5417
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606340.1"
                     /locus_tag="TU31_RS33250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_11_00810"
                     /product="ectoine synthase"
                     /protein_id="WP_018091828.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.8e-55, bitscore:
                     178.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETTLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKYTITPGTMYLLDGHEKHTMRIKEDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPESEPETV"
     gene            5513..6412
                     /gene="thpD"
                     /locus_tag="TU31_RS33255"
                     /old_locus_tag="TPA0598_11_00820"
     CDS             5513..6412
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606339.1"
                     /locus_tag="TU31_RS33255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_11_00820"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_042161640.1"
                     /sec_met_domain="PhyH (E-value: 2.2e-38, bitscore: 126.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTAEVITPRKDPVVWSQPGTAGPFEPSELS
                     DFERDGFFAIGELLTPEEVAVYRAELDRLVLDPTMRADPRSIVEPKSQDVRTVFEVHKI
                     SEVFAKLVSDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYETNGGLMIMPGSHQHFVGCEGETPKDNYKRSLQMQDAGIPSDEV
                     LTKMADKHGIRLFTGQAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENEAVEPFA
                     APVRRPEYIGARDFTPVR"
     gene            complement(6529..7596)
                     /locus_tag="TU31_RS33260"
                     /old_locus_tag="TPA0598_11_00830"
     CDS             complement(6529..7596)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="TU31_RS33260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_11_00830"
                     /product="alkene reductase"
                     /protein_id="WP_042161642.1"
                     /transl_table=11
                     /translation="MTTAFDPIDLAGTPLANRLVMAPMTRNRATAEGVPTPSMVRYYTQ
                     RASAGLIITEASQTEPVGRGYPLTPGLHTPEQVAGWRRVTDSVHAAGGKIFAQLWHGGR
                     IGHPVLLPDGLVPAAPSAVAAAGQGFTQDGPTDFVTPRELTDEEIHRTIEGFAAAARNA
                     LEAGFDGVELHGGNGYLIHQFLAPNTNRRTDAWGGSDEARARFAIEVVKAVAAAAGAHR
                     TGLRLSPGNPFNDIDEPAPEATYTAVVEAVAPLGLAYLHTCDVLDLELLARLRKRFGGT
                     FLLNPATEGRPTGPEELSLVEEGEADLLAYGALFLANPDLPRRLALGGPFNTPDRASFY
                     GGDDSGYLDYPALEA"
     misc_feature    complement(7632..7648)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.66"
                     /tool="antismash"
     gene            complement(7687..8748)
                     /locus_tag="TU31_RS33265"
                     /old_locus_tag="TPA0598_11_00840"
     CDS             complement(7687..8748)
                     /NRPS_PKS="Domain: Aminotran_5 (17-348). E-value: 5.9e-22.
                     Score: 70.0. Matches aSDomain:
                     nrpspksdomains_TU31_RS33265_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606338.1"
                     /locus_tag="TU31_RS33265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_11_00840"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_042161827.1"
                     /sec_met_domain="Aminotran_5 (E-value: 9.8e-17, bitscore:
                     54.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHRREPLGGAEFVPETTFLNTASSGLLPRRSADALRAALEESASY
                     GTMGRDYFAAATASRAAFARLLDVPAERVALGSSVSVQSAFVAATLPEGAEVLAPEGDF
                     SSLVNPFAAHPGIKLRTVPLEALADAVRPGTALVAFSAVQSLDGRIADMAAVREAARAH
                     GARTYLDITQAAGWLPLRAADFDFVVAGAFKWLLCPRGVTFMVFGGDYGRGEEWPAALH
                     AGWVAGEDIGESNYGLIQPAGTARRFDEPYAYFSYVGAEQSLGLLEELGLETIHAHNTA
                     LADHYRAGLAERGLTPRPAPGSAIVSTPGIADAEDRAAAAGVRISVRGGLLRAAFHLYN
                     STDDVDRLLALLG"
     aSDomain        complement(7705..8697)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TU31_RS33265_Aminotran_5.1"
                     /evalue="5.90E-22"
                     /label="TU31_RS33265_Aminotran_5.1"
                     /locus_tag="TU31_RS33265"
                     /protein_end="348"
                     /protein_start="17"
                     /score="70.0"
                     /tool="antismash"
                     /translation="FLNTASSGLLPRRSADALRAALEESASYGTMGRDYFAAATASRAA
                     FARLLDVPAERVALGSSVSVQSAFVAATLPEGAEVLAPEGDFSSLVNPFAAHPGIKLRT
                     VPLEALADAVRPGTALVAFSAVQSLDGRIADMAAVREAARAHGARTYLDITQAAGWLPL
                     RAADFDFVVAGAFKWLLCPRGVTFMVFGGDYGRGEEWPAALHAGWVAGEDIGESNYGLI
                     QPAGTARRFDEPYAYFSYVGAEQSLGLLEELGLETIHAHNTALADHYRAGLAERGLTPR
                     PAPGSAIVSTPGIADAEDRAAAAGVRISVRGGLLRAAFHLYNSTDDVDRL"
     misc_feature    8843..8857
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     gene            complement(8861..9574)
                     /locus_tag="TU31_RS33270"
                     /old_locus_tag="TPA0598_11_00850"
     CDS             complement(8861..9574)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091831.1"
                     /locus_tag="TU31_RS33270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_11_00850"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_042161644.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEAGLAAFAHREGVEVVHRSFELDP
                     NAPAATDIPVVEMLATKYGVSRAQAEAMEARVAEAAAGEGLAYRSDRMHGNTFVLHRLL
                     HLAREHGVQEALLDAFYRANFAEARRLGDPAVLRVTAVGAGLPAEEVARVLADPEAYAE
                     EVRAEEREAAELGATGVPFFVIDRRYGISGGQPAEVFREALERAYADGAEPLRAAGDGG
                     DAGVCGDDGCAVPGK"
     gene            9654..10358
                     /locus_tag="TU31_RS33275"
                     /old_locus_tag="TPA0598_11_00860"
     CDS             9654..10358
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /GO_function="GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA]"
                     /GO_process="GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091833.1"
                     /locus_tag="TU31_RS33275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_11_00860"
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="WP_042161646.1"
                     /transl_table=11
                     /translation="MTSSQGFLGFWETTGSAKTFTHPLDPALLDAYVPRSARVLDYGCG
                     YGRLTAELAGAGYRAVRGVDVSAALIARGKREHPELELLRCPGFPLPFGDGAFDAALLF
                     AVLTCVPRDADQTAIVGELGRLVRPGGVLYLSDVPLQGDAVNRERYARFAERYGTYGVF
                     ETPDGGVFRHHPPERLRGLVREAGFSVVKEREGSVATLDGRTAERLQIIARREPVHPEP
                     VPPEVSRGPASG"
ORIGIN
        1 gcgtacgggg tcggtcagcc gcggccctcg ggtgcggcgc tgcggcgccg gcgggcgaag
       61 accatcgcac cggcgcccgc ggcgagcagc agcccggcgc cgccggcgag gtacggggtg
      121 gccgagctgc cgccggtctc cgcgaggttc tgggtgctgg gcccggagcc cgtccgggac
      181 acctcggtgc ccttgccgcc ggccggctcg gtgccgccgc cggtctggcc gccgccggtc
      241 ccgccggagc cgttgtcctc gggcgccttg ccgcgcggcg tggtgcaggt ggccttggcc
      301 agcgtgaccc ggccgtgcac gtcggcgacg ttcagcttca gcgggttgac ggagacatcg
      361 aggtcgaggg cggtggccgc ggcgcccttg gacgtgacgt tcttcttgga caggtcgagc
      421 cgcacctggc ccacccccgg caccttgacc ttggtggtgc cgcccgcggt gagcgtgacc
      481 ttctggccca ggacgcgcac ggtgcccagc acgttcgact cggcccgggg ccgtgcgccc
      541 gccgcacaga ccgccttggc ggtgacctgc tggacctcga tcacggacag cagcggcaga
      601 ccgggcagat gcaccttcgc ccgcacgagg ttggcgtagc cctcggccgc acggcggtcg
      661 gcggtggccc gcgcggtcgc ggcgtcggcg cgcagcacgc tgaacggcct gccgtggtcg
      721 atgccgtcga gcttgacggt gagcgcggtc ttgttggccg aggcgggggc gtgcacctcg
      781 ttgagggcga cgttcagcgg gacatcgacc gtcttgttga gcagcccgac gttcacgccg
      841 gtgcgcagca cgaccgcgtc ggcggtgccg cgggccgtgc catggtctcc ggtggcatgc
      901 gcggggacgg cgccgaggag gacggcggga ccggccgtga gcgcggtggc gacgagggtc
      961 gcggcgaggc gtcggggcat gccgaaggag ctggtgggac tggtggacac gtgtgtggaa
     1021 ccctcacagg agaaatggag ccgccggtcg cgccaatggg ggacatcgcg cgggccgacg
     1081 gcatcgaccc gcacatcgtg tccgtaccga gggtgaacgg gcagccacgt ggcgtcagtt
     1141 catcccaatg ggtggtttcc gtgcctgtat tcgattcctg cgacacgggg caccaccggc
     1201 gccccagccg ccccacctcc cgggtgaccc gcgttgaccg ggccggttca ccgggtcacc
     1261 ccggcggcta ccccacgatc cgcccccgca gcaccacccg gcgcggtgcc gccagcaccc
     1321 ggacgtccgc gcgcggatcg ctctcgtaga ccaccaggtc cgccggggcg ccctcggtga
     1381 ggccggggcg gccgagccag tcccgggcgc cccaggtggt cgcggagagg gccgccacgg
     1441 cgggcagacc ggcccgggtc agctcgacga cctcctcggc gaccagaccg tgggcgagcg
     1501 agccgccggc gtccgtaccg gcgaagacgg gcactccggc gtcgtaggcc gcccggacgg
     1561 tgtcgtagcg gcggtcgtgc aggcgccgca tgtggtccgc ccagcgcggg aacttcgccg
     1621 cgcccccgtc cgcgagccgc gggaaggtgg cgatgttgac gagcgtggga acgatggcca
     1681 caccgcgctc ggcgaacagc gggatggtct cctcggtgag cccggtggcg tgctcgatgc
     1741 agtcgatacc ggcctcgacg agcggggcga gggtgtcctc ggcgaagcag tgcgcggtca
     1801 cccgggcgcc cagccggtgg gcctcggcga tggcggcctc gaccgctccc cggggccagc
     1861 aggccgccag atcgcccgtg gagcggtcga tccagtcacc gacgagcttg acccagccgt
     1921 cgccgcggcg ggcctcctgg gcgacatagg cgaccaggtc ctccggctcg atctcgtgcg
     1981 cgtagttgcg gatgtagcgc ttggtacggg cgatgtggcg gccggcgcgg atgatccgcg
     2041 gcaggtcctc gcggtcgtcg acccagcggg tgtcggaggg ggacccggcg tcgcggatca
     2101 gcagggtgcc ggcgtcacgg tcggtgagcg cctgcttttc gctggtgggg gcgtccaccg
     2161 ggccgtgggc gtccaggccc acatggcagt gggcgtcgac caggcccggc agggcccagc
     2221 ccgtcacggt ggtggcatcc cgtgccatca ccggccggtc gaaggtcacc cggccgccga
     2281 ccacccacag ctcgtcgcgc acgtcgtccg gtccgacgag gaccctcccc ttgaggtgca
     2341 gcaccgcacc ttcactcatg gacgtcatac cggcactgta ggcggcgtgg cacggcgggt
     2401 ggccggggcg gtcgcgccgg gtgcgcggtc acctttcccc ggccgtttcg cggggcaccg
     2461 ggcgcccgca aggacttttc cgccgggact tttctccgtg cactctccgt gtggcttccg
     2521 ccacccgcgc cgcacggttc tccgcgcgtt cccggcaact ctgcgtacgt ttcgcgcaac
     2581 cttccggcag atcgcctcgc gcatgttctc gcgggtcttg tcacggtgtt gccgcacagg
     2641 tttccatggg cctgcgccag accttccggc ggcgaattcc caattcctca gaagtacagc
     2701 ttcccgtatt cttctgcccg cttttccgca gctcaaacgc caagattttg ctagtggcaa
     2761 gttccgtaat tcggacggat ctcatgggcg ttacggagct gtgaccgact acacaacgtg
     2821 tccgtttcgc cccgaacttc ctggtcaaat cgggacatct cgcggtggca tcgcgcgtac
     2881 gggcgccccc gcgcgataac acatctcggc cgcttccgta acccctgctc gcgatgcaat
     2941 ttcctttttc cctgggtaaa tttaattccc atgaccgccg cacaagcaga ccatgcaggt
     3001 gcccgaagcg atatcagaga atttccggag ggcttcaagc tcgacacccc acgcgtggag
     3061 gacggagccg cgatctggcg catcgcccgc gactcgaagg cgctggatct caactcctcg
     3121 tacagctacc tcctgtggtg tcgcgacttc gccgccacct ccgtcgtcgc gcgcgacgcg
     3181 gagggcgagc cggccgcctt catcaccgga tacctccgcc cggagcgccc ggagaccctc
     3241 gtcgtctggc aggtcgccgt cgacgacgcg caccgtgggc agggactggc ggccgcactc
     3301 ctggacgggc tcgccgcccg ggccagggac gagctgggcg tacgtttcgt cgagaccacc
     3361 gtgacccccg acaacaccgc atccaaccgg ttgttcgcgt ccttcgccga gcggcactca
     3421 gtgcctctca agagcgaggt gctctttgac gcggggctgt tcccggagca gggccacgag
     3481 ccggaggtgc tgcacctcat cggcccgttc gagccaccgg cccgcacggg gcactcctga
     3541 cgcacccagg accgcgcggc gacgacgccg cagcggtcat gcgggacccg gcaccacacc
     3601 cccgtgacac cgccggcacc gcccagcccc ctacagactt ctcatccttt cgccactcat
     3661 ctcccaggag catgctgtga ccatcaccca gcccgatctg agcgtcttcg agaccgtgga
     3721 gtcggaggtg cgcagctact gccgtagctg gcccaccgtc ttcgaccgtg cgcagggcag
     3781 ccgcatgacc gacgaggacg gccacaccta cctcgacttc ttcgccgggg ccgggtcgct
     3841 caactacggc cacaacaacc cggtcctcaa acgcgccctg atcgactaca tcgagcggga
     3901 cggcgtcacc cacggcctgg acatgtccac cacggccaag cgggccttcc tggagtcgtt
     3961 ccagaacatc atcctgcggc cccgtgacct gccctacaag gtcatgttcc cgggcccgac
     4021 gggcgccaac tccgtcgagg cggcgctcaa gctggcccgt aaggtcaagg gccgcgagtc
     4081 gatcgtgtcc ttcaccaacg ccttccacgg catgtcgctg ggcgccctcg cggtcaccgg
     4141 taactccatg aagcgggccg gcgccggtat cccgctggtg cacggcaccc cgatgccgtt
     4201 cgacaactac ctcgacggca cctacccgga cttcctgtgg ttcgagcggc tgctggagga
     4261 ccagggctcc ggtctgaacc ggcccgcggc cgtgatcgtc gagacggtcc agggcgaggg
     4321 cggcatcaac gtcgcccgtg ccgagtggct gcgcgccctc tccgagctgt gcgagcgctg
     4381 ggacatgctg ctcatcgtcg acgacatcca gatgggctgc ggccgtacgg gtgccttctt
     4441 ctccttcgag gaggcgggca tcgtgccgga catcgtcacc gtctcgaagt ccatcagcgg
     4501 ctacgggctg ccgctcgcgc tcaccctgtt caagcctgag ctggacatct gggagccggg
     4561 cgagcacaac ggcaccttcc gcggcaacaa cccggccttc gtgaccgccg ccgcgacgct
     4621 ggacacctac tgggccgacg gccagatgga gaagcagact ctcgcccgcg gtgagatcgt
     4681 cgaggcacac ctgaaggcga tcgccgagga gcacccggac accatcgtcg agtaccgcgg
     4741 ccgcggtctc gtgtggggtc tggagtgcac ccccaagccc ctggccgaca agatcgcagc
     4801 ccgcgctttc gagctgggcc tgctgatcga gacctccggc ccggagagcg aggtcgtcaa
     4861 actgctcccc gcactgacga ccacccccga ggaactggac gagggactcc gcatcctcgc
     4921 ccgcgcggtc cgcgactgcg cctgatcggc ccaacggccc cagagatcct taccgttcca
     4981 cagaaaggca ccgagtcacc gtgatcgtcc gatccttcaa ggacatcgaa ggcaccgacc
     5041 gccacgtcaa ggccaagtcc ggcacctggg agagcaagcg catcgtgctc gccaaggagc
     5101 gcgtcggctt ctcgctgcac gagaccacgc tgtacgccgg gaccgagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtactctgcg tagagggtga ggccgagctc acgaacgatg
     5221 agaccggcga gaaatacacc atcacgccgg gcacgatgta cctgctcgac gggcacgaga
     5281 agcacaccat gcggatcaag gaggacttcc gctgcgtgtg tgtcttcaac ccgcccgtca
     5341 ccggccgcga ggaccacgac gagaacgggg tctacccgct tctcaccgaa ccggagtctg
     5401 agcccgagac ggtctgaggc gccgcccgcc cgctgccccg tccggcagcg cccaggaggc
     5461 acgcacacgc acgtaagcca gattccgtac gagaggagag gaaggcaaca ccatgaccac
     5521 cgcacccgag cgcaccgccg acctgtaccc gacccgtggg accgctgagg tcatcacccc
     5581 gcggaaggac ccggtggtgt ggtcgcagcc cggaacggcc ggccccttcg agccgtccga
     5641 gctgagcgac ttcgaacgcg acggcttttt cgccatcggg gaactgctca cgccggaaga
     5701 agtcgcggtg taccgcgccg aactggaccg gctggtgctc gacccgacga tgcgcgccga
     5761 cccgcgctcc atcgtcgagc cgaagtcaca ggacgtacgg accgtgttcg aggtgcacaa
     5821 gatcagcgag gtgttcgcca agctggtctc cgatccgcgg gtggtcgggc gggcccgcca
     5881 gatcctcggc tcggacgtgt atgtccacca gtcgcggatc aatgtgaagc cgggcttcgg
     5941 tgcctccggc ttctactggc actccgactt cgagacctgg cacgccgagg acgggctgcc
     6001 gaacatgcgc acggtgtcgg tctcgatcgc gctgacggag aactacgaga ccaacggcgg
     6061 cttgatgatc atgcccggct cgcaccagca cttcgtgggc tgtgagggcg agacgccgaa
     6121 ggacaactac aagcggtcac tccagatgca ggacgcgggc atcccgtcgg acgaggtcct
     6181 gacgaagatg gcggacaagc acgggatccg cctcttcacg ggtcaggccg gctcggcgac
     6241 gtggttcgac tgcaacgcga tgcacggctc gggcgacaac atcacgccct acccgcgcag
     6301 caatgtcttc atcgtcttca acagcgtgga gaacgaagcg gtggagccgt tcgcggcacc
     6361 ggtccggcgc ccggaataca tcggggcccg cgacttcact ccggtgcgtt aggggacgtt
     6421 cgcaccggtg gcgcggccgc cgcacagcag gtctgtgcgg cggccgcgcc gtgttcaccg
     6481 gccggtgcgc ggggccccac cgggcgcccg cacccggcgg ccggttcctc aggcctccag
     6541 cgccgggtag tcgaggtagc cggagtcgtc accgccgtag aagctcgccc ggtccggggt
     6601 gttgaagggg ccgccgagcg ccagccgccg gggcagatcg gggttggcga ggaacagtgc
     6661 gccgtacgcc agcagatcgg cctcgccctc ctcgacgaga gacagctcct cgggaccggt
     6721 gggccggccc tcggtggccg ggttgagcag gaacgtcccg ccgaagcgct tgcgcagccg
     6781 ggccagcagc tccaggtcga gcacatcgca ggtgtgcaga taggccaggc ccagcggggc
     6841 gacggcctcg accacggccg tgtaggtggc ctccggcgcg ggctcgtcga tgtcgttgaa
     6901 ggggttgccg ggcgacagcc gcagaccggt gcggtgcgca ccggccgcgg cggcgacggc
     6961 cttgacgacc tcgatggcga agcgggcgcg ggcctcgtcg gagccgcccc aggcgtccgt
     7021 gcgccggttg gtgttgggcg ccaggaactg gtggatcaga tagccgttcc cgccatggag
     7081 ctccacaccg tcgaaccctg cctccagggc gttgcgggcc gcggccgcga acccctcgat
     7141 ggtccggtgg atctcctcgt cggtcagctc gcgcggggtg acgaagtccg tcgggccgtc
     7201 ctgggtgaag ccctggccgg ccgcggcgac cgcggagggc gcggcgggca ccaggccgtc
     7261 gggcagcagc accgggtgac cgatacggcc gccgtgccac agctgggcga agatcttgcc
     7321 gcccgcggcg tgcacggagt cggtgacccg gcgccatccg gcgacctgct ccggggtgtg
     7381 cagtcccgga gtgagcgggt aaccacggcc gaccggctcg gtctgcgagg cctcggtgat
     7441 gatgagtccg gcggaggccc gctgggtgta gtagcggacc atcgacgggg tcgggacgcc
     7501 ctcggcggtg gcgcggttgc gggtcatcgg ggccatgacg aggcggttgg cgagcggggt
     7561 gccggcgagg tctatcgggt cgaacgcggt ggtcactggg ggcctttccc tgctgtccgg
     7621 aacaagatcg ttgcccatta catcagctgc cgtacgcccc cacccgcccc ctgttccccg
     7681 gccgcgctac cccagcaggg ccagcaaccg gtccacgtca tccgtcgagt tgtagaggtg
     7741 gaaggccgcg cggagcagcc cgccgcgcac cgagatccgc acccccgccg cggcggcccg
     7801 gtcctccgcg tccgcgatcc cgggggtgga gacgatcgcc gaaccgggcg cgggccgcgg
     7861 cgtcagcccc cgctcggcca gccccgcacg gtagtggtcg gcgagcgcgg tgttgtgggc
     7921 gtggatggtc tccaatccca gctcctccag gaggccgagg gactgctcgg cgccgacgta
     7981 ggagaagtac gcgtacggtt cgtcgaagcg ccgggcggtg ccggccggct ggatcagccc
     8041 gtagttggac tcgccgatgt cctcccccgc gacccagccc gcatgcagcg cggccggcca
     8101 ctcctcgccc cgcccgtagt ccccgccgaa caccatgaag gtcaccccgc gcgggcacag
     8161 cagccacttg aacgccccgg cgacgacgaa gtcgaagtcg gcggcgcgca gcggcagcca
     8221 gccggcggcc tgggtgatgt cgaggtaggt acgcgccccg tgggcccggg cggcctcgcg
     8281 caccgccgcc atgtcggcga tccggccgtc cagcgactgc acggcactga aggcgaccag
     8341 cgcggtgccg gggcgcacgg cgtcggcgag cgcctccagc ggcacggtgc gcagcttgat
     8401 gcccgggtgg gcggcgaacg gattgaccag ggagctgaag tcgccctcgg gggccagtac
     8461 ttcggcgccc tccgggagcg tggcggcgac gaacgcggac tgcaccgaga ccgaactgcc
     8521 gagggcgacc cgctcggcgg gcacgtccag cagccgggcg aaggcggcgc gggaggcggt
     8581 ggcggcggcg aagtagtcgc ggcccatggt gccgtaggag gcggactcct ccagggccgc
     8641 gcgcagggcg tcggcgctgc gccgcgggag gagtccgctg gaggcggtgt tcagaaaggt
     8701 cgtctcgggc acgaattcgg ccccgccgag tggctcacgt cgatgcatgt ccccactctg
     8761 ctcggccccg cacgccacgt ccatggcgca ggtgctcggc cggcccgcaa cagctcctta
     8821 tcggcgcagg tcacgggggt gcgggcggac cggccccggt tcacttcccg ggcacggcgc
     8881 agccgtcgtc cccgcacacc ccggcgtccc cgccgtcccc ggccgcccgg agcggttccg
     8941 ccccgtcggc gtacgcacgc tccagggcct cccggaagac ctcggcgggc tgcccgccgg
     9001 agatgccgta ccggcggtcg atgacgaaga acggcacccc cgtggcaccc agctccgcgg
     9061 cctcccgctc ctcggcgcgc acctcctccg catacgcctc ggggtcggcc agcacccgcg
     9121 cgacctcctc ggcgggcagc cccgcaccga cggcggtcac acggagcaca gcgggatcac
     9181 cgagccgcct ggcctcggcg aagttggccc ggtagaaggc atcgagcagc gcctcctgga
     9241 caccgtgctc ccgggcgagg tgcagcagcc ggtgcagaac gaaggtgttg ccgtgcatcc
     9301 ggtcggaccg gtacgccagc ccctcccccg ccgccgcctc ggcgacccgg gcctccatcg
     9361 cctcggcctg cgcccggctc acgccgtact tcgtggcgag catctcgacc accgggatgt
     9421 cggtggcggc cggcgcgttc ggatccagct cgaaggagcg gtgcaccacc tcgaccccct
     9481 cccggtgcgc gaaggccgca agcccggcct cgaagcgggc cttgccgatg tagcaccagg
     9541 gacaggcgat atcggaccag atttcgacgc gcatgagctg actttcctcg gggtggacgt
     9601 gcgggggtgc aacgtcggtg ggcggcgggc tattccgtgg gtaccgtccc ggcatgacct
     9661 cgtcacaagg atttctcggg ttctgggaga cgaccgggtc cgccaagacc ttcacccacc
     9721 cgctcgaccc ggcgctgctg gacgcgtatg tgccgcggag cgcgcgggtg ctggactacg
     9781 gatgcggcta cgggcggctg accgccgagc tggccggggc cgggtaccgc gcggtgcgtg
     9841 gggtggatgt ctcggcggcg ctgatcgcgc gggggaagcg ggagcatccg gagctggagc
     9901 tgctgcgctg tcccggtttt ccgctgccgt tcggggacgg tgcttttgat gcggcgctgc
     9961 tcttcgcggt gctgacctgt gttccccggg acgcggacca gacggcgatc gtcggggagc
    10021 tggggcggct ggtgcggccg ggcggggtgc tgtatctgag cgatgtgccg ttgcaggggg
    10081 atgcggtcaa ccgggagcgg tatgcgcggt tcgcggagcg gtacgggacg tacggggtgt
    10141 tcgagacgcc cgacggcggg gtgttccggc accatccgcc ggagcggctg cgggggctgg
    10201 tgcgggaggc gggcttctcg gtggtgaagg agcgggaggg gtcggtcgcc acgctggacg
    10261 gccggacggc cgagcggctt cagatcatcg cccggcggga gccggtgcac ccggagccgg
    10321 tgccgccgga ggtgtcgcga ggtccagcgt cagggtgagg tgggtggcgt ccggagcggg
    10381 gcggcggtcc gggtcggggc gccagccgag ccgggtg
//
