LOCUS       NZ_BBNO01000008        10981 bp    DNA     linear   CON 19-AUG-2024
DEFINITION  Streptomyces lydicamycinicus strain TP-A0598, whole genome shotgun
            sequence.
ACCESSION   NZ_BBNO01000008
VERSION     NZ_BBNO01000008.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces lydicamycinicus
  ORGANISM  Streptomyces lydicamycinicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Komaki,H., Hosoyama,A., Igarashi,Y. and Tamura,T.
  TITLE     Streptomyces lydicamycinicus sp. nov. and Its Secondary Metabolite
            Biosynthetic Gene Clusters for Polyketide and Nonribosomal Peptide
            Compounds
  JOURNAL   Microorganisms 8 (3), 370 (2020)
   PUBMED   32155704
  REMARK    DOI:10.3390/microorganisms8030370 Publication Status: Online-Only
REFERENCE   2
  AUTHORS   Komaki,H., Ichikawa,N., Hosoyama,A., Fujita,N. and Igarashi,Y.
  TITLE     Draft genome sequence of marine-derived Streptomyces sp. TP-A0598, a
            producer of anti-MRSA antibiotic lydicamycins
  JOURNAL   Stand Genomic Sci 10, 58 (2015)
   PUBMED   26380643
  REMARK    DOI:10.1186/s40793-015-0046-5 Publication Status: Online-Only
REFERENCE   3  (bases 1 to 724454)
  AUTHORS   Hosoyama,A., Kimura,A., Ohji,S., Ichikawa,N., Yamazoe,A. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2014) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Newbler v. 2.6
            Genome Coverage                   :: 93x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina HiSeq 1000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000829715.2-RS_2024_08_19
            Annotation Date                   :: 08/19/2024 02:08:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,323
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,092
            CDSs (with protein)               :: 7,092
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 4, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 54 of 145
            Pseudo Genes (incomplete)         :: 123 of 145
            Pseudo Genes (internal stop)      :: 9 of 145
            Pseudo Genes (multiple problems)  :: 39 of 145
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:07:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 485596
            Orig. end                         :: 496577
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBNO01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10981
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="3"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10981
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10981
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5981](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5981
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            248..1099
                     /locus_tag="TU31_RS28395"
                     /old_locus_tag="TPA0598_08_04190"
     CDS             248..1099
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088832.1"
                     /locus_tag="TU31_RS28395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_04190"
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="WP_042159640.1"
                     /transl_table=11
                     /translation="MNDPHTQRTPRSLIVTFYGAYGRGEPGGPAGPAAPVPVAALIRLL
                     GALGVDPPSVRSAVSRLKRRELLTPARTATGAAAYGLSDAARQLLDDGDRRIFGRPAAR
                     LSDGWVLAVFSVPEEERHKRHLLRSRLARLGFGTAAPGVWIAPAQLYEETRHTLERLQL
                     DPYVDLFTGAHAGFAPTADAVARWWDLDAIAAQHRAFLEEQEPVLQRWSRRRSVPPEAA
                     YRDYLLALDAWRRLPYADPGLPSPLLPEDWPGGRAADVFGRLHAKLRDAGAPYAHEAMA
                     GA"
     misc_feature    285..299
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.44"
                     /tool="antismash"
     gene            complement(1119..1883)
                     /locus_tag="TU31_RS28400"
                     /old_locus_tag="TPA0598_08_04200"
     CDS             complement(1119..1883)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536998.1"
                     /locus_tag="TU31_RS28400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_04200"
                     /product="HAD family hydrolase"
                     /protein_id="WP_052719089.1"
                     /sec_met_domain="HAD_2 (E-value: 1.8e-28, bitscore: 95.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVATEVGERPVRGVRGAGGETIRAAVFDLDGTLANTLPAISKLL
                     IKVASEQGCSVTARQAAAAIGKPPGPAFGRLLGRPEDDGRVQAAISRYRELFAYEVLCL
                     GPQLLFPGVTAGLSALRAHGVELAVATSKTTRSAEALLDVMGIRDLVGPVIGQDMVRRG
                     KPHPEMVLRAAGRLGVAAWQSAYVGDTVGDMRMAVTARMEPVAVTYGVGTFGELAGVAG
                     TRMCGSFKDVVSVIAAARPRSGVKVGAQQRRR"
     misc_feature    complement(2047..2061)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.22"
                     /tool="antismash"
     misc_feature    complement(2073..2087)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     gene            2335..2967
                     /locus_tag="TU31_RS28405"
                     /old_locus_tag="TPA0598_08_04210"
     CDS             2335..2967
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088830.1"
                     /locus_tag="TU31_RS28405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_04210"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_042159644.1"
                     /transl_table=11
                     /translation="MPTQHRAIQSRQALIRSAAETFLEKGVPAAGMVEISRRARLSKGA
                     LYFHFTSKDDLTLAVRDAALEALQELEDAFTRSTQPLTTAVRDFTTALFGRVESDAVLR
                     AGLRLRPETAPGLGIYALEQRWYALFLDKAVTCGTGGPAGPHATDTEPRRTAQLLTSIV
                     VGLLHLGGEDGTWWDREAIVDLWALLPAAPGRIPAAVPAPAQLPAAG"
     gene            complement(3000..4028)
                     /locus_tag="TU31_RS28410"
                     /old_locus_tag="TPA0598_08_04220"
     CDS             complement(3000..4028)
                     /NRPS_PKS="Domain: PKS_ER (80-340). E-value: 8.6e-24.
                     Score: 76.1. Matches aSDomain:
                     nrpspksdomains_TU31_RS28410_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088829.1"
                     /locus_tag="TU31_RS28410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_04220"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_042159646.1"
                     /sec_met_domain="ADH_zinc_N (E-value: 8.4e-19, bitscore:
                     63.1, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPTVVPAIPAVHREVRLADRVEGELTARHFTIAEVPVPEPRDGE
                     VLVRNRLMAVTAVMRSQMTGIRLPMPSFVPGEALWGAAVGEVVAAPGGGFTPGDLVQHP
                     CGWREFAAVDASRVRRLDPDALPTAAAHLSQGATAWGALRRAAEVRPGDTVFISGAAGG
                     VGSMAGQLARRLGAGRVVGSTGSERKAARLRAELGYDDTIVRGAGPIERQLRRAAPGGI
                     DVLLDTVGGEQLTAALAVARPDARFALVGALSGQLGGDRGDIAPVELDPGLIITRRVAL
                     RGFGLHAHPDLPQEWTKEFGQGLREGTLVFPHALLKGIEQAPQALRELTQGRHIGAVLV
                     AL"
     aSDomain        complement(3009..3788)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TU31_RS28410_PKS_ER.1"
                     /evalue="8.60E-24"
                     /label="TU31_RS28410_PKS_ER.1"
                     /locus_tag="TU31_RS28410"
                     /protein_end="340"
                     /protein_start="80"
                     /score="76.1"
                     /tool="antismash"
                     /translation="GAAVGEVVAAPGGGFTPGDLVQHPCGWREFAAVDASRVRRLDPDA
                     LPTAAAHLSQGATAWGALRRAAEVRPGDTVFISGAAGGVGSMAGQLARRLGAGRVVGST
                     GSERKAARLRAELGYDDTIVRGAGPIERQLRRAAPGGIDVLLDTVGGEQLTAALAVARP
                     DARFALVGALSGQLGGDRGDIAPVELDPGLIITRRVALRGFGLHAHPDLPQEWTKEFGQ
                     GLREGTLVFPHALLKGIEQAPQALRELTQGRHIGAVLV"
     CDS_motif       complement(3513..3575)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TU31_RS28410_0001"
                     /evalue="1.70E-04"
                     /label="PKSI-ER_m5"
                     /locus_tag="TU31_RS28410"
                     /protein_end="172"
                     /protein_start="151"
                     /score="14.3"
                     /tool="antismash"
                     /translation="GDTVFISGAAGGVGSMAGQLA"
     misc_feature    4122..4140
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.84"
                     /tool="antismash"
     gene            4257..4853
                     /locus_tag="TU31_RS28415"
                     /old_locus_tag="TPA0598_08_04230"
     CDS             4257..4853
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602525.1"
                     /locus_tag="TU31_RS28415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_04230"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_245698820.1"
                     /transl_table=11
                     /translation="MRTRRAVLEAAAHVIGTRGYQAATMAEIIQRAGVTKGAVYFHFTS
                     KDALARAVITEQTDPFLPQVSESRLQDAIDFTHQVALALRSDPLLQAGTRIAVETTFSE
                     DPLVPYQAWTDIITTMFTEARDNGELLPGVAPDRAAEFFVAAYMGVQLFSRAATNRADL
                     PERVTALWKHTLPGLASPGALSHLDPHGRCVKVSV"
     misc_feature    4644..4646
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5001..5981
                     /locus_tag="TU31_RS28420"
                     /old_locus_tag="TPA0598_08_04240"
     CDS             5001..5981
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088827.1"
                     /locus_tag="TU31_RS28420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_04240"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_052719090.1"
                     /sec_met_domain="AfsA (E-value: 1.5e-23, bitscore: 77.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASATLTPYLRDQRHDIGGGSADGIRPSSLTTTVPREYVHRNALS
                     EVFLTNWRRGGADTWVVSAQWPRAHTFYGPLHGMHDPILLIETVRQAGILLSHVAHRVP
                     LDHPIIWQRVRYDLAPQALRTAQHPAEVELHITDRDMVHRGKRLVSALQEFRIRCDGAE
                     LASATLDYSCHSPAVYRRLRGEYGDLAFANSRTLPLPGAMSPQLVAREHDRDVVLSPTD
                     RPDRWQLRVDTSHPVLFDHPVDHAPGMLMVEAARQAAQAATPGVTLPVSMECSFSRYAE
                     LDAPIWVQARTTGRGDDDRRQVEVTVEQHGRPVVSAQVSCVPTPP"
     misc_feature    5283..5285
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6105..8396)
                     /locus_tag="TU31_RS28425"
                     /old_locus_tag="TPA0598_08_04250"
     CDS             complement(6105..8396)
                     /GO_function="GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088826.1"
                     /locus_tag="TU31_RS28425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_04250"
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="WP_042159650.1"
                     /transl_table=11
                     /translation="MRVAVIGGGPGGLYAAVLLKRLDPTREITVWERNAPEDTFGFGVV
                     LSDETLGGIEHADPEVYRALQAEFVRWDDIDIVHRGRTLTSGGHGFAALGRRRLLAVLH
                     RRCRDLGIELRFRTEAPPAAELARAYDLVIAADGVHSATRTAHADAFHPQLTTHRCRYI
                     WLAADFAFDAFRFEIAETEHGVVQLHAYPFARPEGPADGASTVIVEMREEVWRSAGLDR
                     CDERESAEWCAKVFTDALRGRSLRGNNSSWIAFRTVVNDRWSHGNTVLLGDAAHTAHFS
                     IGSGTKLAVEDALALAACLEEQPDTASALAAYEEERRPVVASTQRAARASLAWFEELAT
                     YLDQPPHQFAFNLLTRSRRVTHDNLRLRDPGFVAAVEDEAGTPPGTPPMFLPFRLGELT
                     LRNRVVVSPMDMYSAADNNGTPGDFHLVHLGARALGGAGLVMTEMVCVSPEGRITPGCT
                     GLYAPEHETAWRRVTDFVHTSAPGTAIGVQLGHSGRKGSTRLMWDGIDQPLPDGNWPLV
                     APSALPYRPGVNQTPHALTEPELGTLRAQFVRAAESAARAGFDLLELHCAHGYLLSGFL
                     SPLTNHRTDAYGGDLTGRLRFPLEVFDAVRAVWPAGRPMTVRISATDWADGGTTAEDAV
                     AMARAFAEHGADAIDVSTGQVVPDERPDFGRSYQTPFADRIRNTLGVPVIAVGAISSWD
                     DVNSLVLAGRTDLCALARPHLYDPHWTLHAAAEQGYAGPGAPWPLPYQAGSRTPPTGRL
                     DAPKPRLTLR"
     gene            complement(8399..9226)
                     /locus_tag="TU31_RS28430"
                     /old_locus_tag="TPA0598_08_04260"
     CDS             complement(8399..9226)
                     /NRPS_PKS="Domain: ECH (19-267). E-value: 1.6e-51. Score:
                     166.9. Matches aSDomain: nrpspksdomains_TU31_RS28430_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445228.1"
                     /locus_tag="TU31_RS28430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_04260"
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="WP_042159652.1"
                     /transl_table=11
                     /translation="MSPFAGSARSTETWQHLRVTRQDGVATVTLARPDKLNALTFEAYA
                     DLRDLLAELSRERSVRALVLAGEGRGFCSGGDVEEIIGATLGMDTAQLLDFNRMTGQVV
                     RAVRECPFPVIAAVHGVAAGAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYL
                     LPRVIGLGHATRLLMLGDTVRAPEAERLGLISELAEEGRVDEAAAALARRLAEGPAMAH
                     AQTKALLTAELDMPLAAAVEMDAATQALLMNSDDYAEFHAAFTEKRAPKWQGK"
     aSDomain        complement(8426..9169)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TU31_RS28430_ECH.1"
                     /evalue="1.60E-51"
                     /label="TU31_RS28430_ECH.1"
                     /locus_tag="TU31_RS28430"
                     /protein_end="267"
                     /protein_start="19"
                     /score="166.9"
                     /tool="antismash"
                     /translation="TRQDGVATVTLARPDKLNALTFEAYADLRDLLAELSRERSVRALV
                     LAGEGRGFCSGGDVEEIIGATLGMDTAQLLDFNRMTGQVVRAVRECPFPVIAAVHGVAA
                     GAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYLLPRVIGLGHATRLLMLGDT
                     VRAPEAERLGLISELAEEGRVDEAAAALARRLAEGPAMAHAQTKALLTAELDMPLAAAV
                     EMDAATQALLMNSDDYAEFHAAFTEK"
     misc_feature    complement(9306..9326)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            9529..10335
                     /locus_tag="TU31_RS40680"
                     /old_locus_tag="TPA0598_08_04270"
     CDS             9529..10335
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="TU31_RS40680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="TPA0598_08_04270"
                     /product="ATP-binding protein"
                     /protein_id="WP_052719091.1"
                     /transl_table=11
                     /translation="MLEPPTPATWRIALPRGATAVPIARAMVRTAMQDLRVTADRTTAQ
                     LLTAELVTNVLKHTCGADPIELVVEQRPTGCRIEVHDDSPLLVKGLGDPVPLGEPGAGV
                     VGAPGGGAPDGPGVDPPGGISAGPSAGPSPGRSSDGEVPTQRAEEEAGRSADGAETGDG
                     GEGQELDGEGQGPDEDNGHAGVDGDTYADGSPDSRSADDDDADATAAHGTDTGPDALSP
                     GPARGDHDGRGLLLIRSLSAASGCRPTPRGKAVWFTLPGVRRPRRR"
     misc_feature    10799..10810
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
ORIGIN
        1 gtgaccgagg cggcggtggt cggccggccc gacgaggacc gcgggcaggt ggtcgtggcc
       61 catgtcgtgc tgcgggacgg cgtcccccgg ggtgacgaca ccgtcgtcgc gctgcgcgcc
      121 ttcaccaaga cccgtatcgc gccctacaaa tgcccgcgcg agatcgtctt ccacagctcg
      181 ctgccgcgca ccccgaccgg caagctccag cgtttccggc tgcgggaacc cgatctagag
      241 tgacccggtg aacgacccgc acacacagcg caccccacgg tccctgatcg tcacgttcta
      301 cggcgcctac ggacgtggcg agcccggcgg cccggccggc ccggccgctc cggtaccggt
      361 cgccgccctg atccggctgc tcggtgcgct cggggtcgat ccgccgtcgg tgcgctcggc
      421 ggtctcccgg ctcaagcggc gcgagctgct gactcccgcc cggacggcca ccggcgcggc
      481 cgcgtacggg ctgtccgacg cggcccgcca gctgctggac gacggcgacc gccggatctt
      541 cggccggccg gccgcgcggc tgtccgacgg ctgggtactg gccgtcttct ccgtccccga
      601 ggaggagcgg cacaagcgcc atctgctgcg ctcccggctg gcccggctcg gcttcggcac
      661 cgccgcgccg ggtgtgtgga tcgcccccgc gcagctctac gaggagaccc ggcacaccct
      721 ggagcggctg caactggacc cgtacgtcga cctgttcacc ggtgcgcacg ccggcttcgc
      781 ccccacggcg gacgcggtcg cccgctggtg ggacctggac gccatcgccg cacagcaccg
      841 cgccttcctg gaagagcagg agccggtcct ccagcggtgg tcacggcgcc gctcggtgcc
      901 ccccgaagcg gcctaccgcg actatctgct ggcgctggac gcctggcgcc ggctgcccta
      961 cgcggatccc ggactgccct ctcccctcct gcctgaggac tggccgggcg gccgcgcggc
     1021 ggacgtgttc ggccggctgc acgccaaact ccgggacgcc ggggccccct acgcgcacga
     1081 ggcgatggcc ggggcgtagc ggccccagcg caccggcctc agcggcggcg ctgctgggcc
     1141 cccaccttga cgcccgagcg cggccgggcg gcggcgatca cggagaccac gtccttgaag
     1201 gagccgcaca tccgggtgcc ggcgaccccg gcgagctcac cgaaggtgcc gaccccgtag
     1261 gttaccgcga ccggctccat gcgcgcggtg accgccatcc tcatgtcccc cacggtgtcc
     1321 cccacatacg cgctctgcca ggccgcgaca ccgagccggc cggccgcccg cagcaccatc
     1381 tccgggtgcg gtttgccgcg ccggaccatg tcctggccga tgaccgggcc gaccaggtcg
     1441 cggatgccca tgacgtcgag cagcgcctcc gcgctgcggg tcgtcttgga cgtggcgacg
     1501 gccagctcga ccccgtgggc gcgcagcgcg gacagccccg cggtcacgcc cgggaagagc
     1561 agctgcggcc ccaggcacag cacttcgtag gcgaacagtt cgcggtagcg ggagatcgcg
     1621 gcctgcaccc ggccgtcgtc ctcgggcctg cccagcagcc ggccgaaggc ggggccggga
     1681 ggcttgccga tcgcggcggc cgcctgccgt gcggtcaccg aacacccctg ctccgaggcc
     1741 accttgatca gcagtttgct gatcgcgggg agggtgttgg cgagggtgcc gtccaggtcg
     1801 aagacggcgg cccggatcgt ctcacctccg gcgccccgta cacctctcac gggacgttcg
     1861 ccgacttccg ttgctacgcg catggctgga gacctttccg gattccgatg gggggtgcag
     1921 gggctcgggt gacgttgctg gctcggctga tggggcggcc tgcggtcgat gacgggtaac
     1981 ggcagcggcg gtctcgccat ggcgcaccgc cgtccagggc ggcacgcaac cgtcgaggcg
     2041 gcgcatcgga agggaacgtc ccgtttaccg ccttcggaga tttaaaaacc gcctgcgctg
     2101 tttgtcaatg catccccgtc tcccctgacg ggtcgacaac acccctttgc ggcgccagac
     2161 tgggggcaca tgccccgcgg gagccgtcga gcgccggaac gagcgaaaga tcgtcacctt
     2221 cgcgtcaggg cccgccagtt ccggccaatc ccgtggagca tatcgaaccg catggttggt
     2281 tttctatgta gactgcaatg agctcgcagc cgtactagag acgggacgga caccgtgccc
     2341 acccagcatc gcgccatcca gagccgccag gcactcatcc gctcggccgc agagaccttc
     2401 ctggagaagg gggttcccgc agcgggcatg gtcgagatca gccggcgggc ccggctgagc
     2461 aagggagccc tctacttcca cttcacgtcg aaggacgacc tcacgctcgc ggtgcgggac
     2521 gcggcgctgg aggccctaca ggagctcgaa gacgccttca cccgctccac gcagccgctg
     2581 accaccgccg tacgggactt caccaccgcg ctgttcggcc gggtggagtc ggacgcggtg
     2641 ctccgcgccg gcttgcggct gcggccggag accgcgccgg ggctcggtat ctacgccctg
     2701 gagcagcgct ggtacgcgct gtttctcgac aaagcggtga cctgcgggac cggcgggccg
     2761 gccgggccgc atgcgacgga caccgaaccg cggcggacgg cccagctgtt gacctcgatc
     2821 gtggtcggtc tgctgcatct gggcggtgag gacgggacgt ggtgggaccg cgaggccatc
     2881 gtcgacctgt gggcgctgct gccggccgca ccgggccgga tcccggccgc cgtcccggct
     2941 ccggcgcagc ttccggcggc cggctgaacc gggcccgccc cggtcctcag gccccggcct
     3001 cacaacgcga ccagcacggc cccgatatga cgaccctgcg tcagttcgcg cagcgcctgc
     3061 ggcgcctgtt cgatcccctt cagaagggcg tgcgggaaga ccagcgtgcc ctcccgcagg
     3121 ccctggccga actccttcgt ccactcctgc ggcaggtccg ggtgggcgtg cagcccgaac
     3181 ccgcgcaggg ccacccgccg cgtgatgatc agccccgggt ccagctccac cggggcgatg
     3241 tcgccgcggt ccccgcccag ctgaccggag agcgcgccga ccagcgcgaa ccgcgcatcg
     3301 gggcgggcca ccgcgagcgc cgccgtcagc tgttcgccgc cgacggtgtc cagcagcaca
     3361 tcgataccgc ccggcgcggc ccgccgcagc tgccgctcga tcggcccggc gccgcgcacg
     3421 atcgtgtcgt cgtagcccaa ctcggcgcgc agccgcgcgg ctttgcgctc cgagccggtg
     3481 ctgccgacga cccgcccggc acccagccgc cgcgcgagct gcccggccat gctgcccacg
     3541 ccgcccgccg cgccactgat gaacacggtg tcccccggcc tgacctcggc ggcccggcgc
     3601 aaggcacccc aggcggtcgc cccctgcgac agatgcgcgg cagcggtcgg cagcgcgtcc
     3661 gggtcgaggc gccgtacgcg cgacgcgtcg accgctgcga actcccgcca gccgcagggg
     3721 tgctggacca gatcgccggg ggtgaagccg cctccgggcg ccgccaccac ctcgccgacc
     3781 gccgcgcccc acagcgcctc acccggtacg aatgagggca tcggcagccg gattccggtc
     3841 atctgcgacc gcatcaccgc ggtcaccgcc atcaggcggt tgcgcaccag cacctctccg
     3901 tcccgcggct cgggcaccgg gacctcggcg atcgtgaagt ggcgtgccgt cagctcgcct
     3961 tcgacccggt ccgcgagccg gacctcgcgg tgtacggcgg gtatggcggg gacgacggtg
     4021 ggggacatcg gacagctcct cgcgctcgta gcactcgatg atgccgcccc ggaggacgtc
     4081 accggttcac gcccgagggg ccccgcctcc ggaccgggcg caaattcaat accgcatggt
     4141 tggcttcttt atgatgcgga aatagactgc gcggccacgg gcagcgggtc gcactcaggc
     4201 acaacaactc caagagagca tacggaggat gatcggtggt caagcaggaa cgcgcagtgc
     4261 gcacccgacg cgccgtcctc gaagcggcgg ctcatgtcat cggcacccgc gggtaccagg
     4321 ccgccacgat ggccgagatc atccagcgcg ccggggtcac caaaggagcg gtgtacttcc
     4381 acttcacgtc caaggacgca ctggcccgcg cggtcataac ggagcagacc gatccctttc
     4441 tgccgcaggt cagcgagtcc cggctccagg acgccatcga cttcacccac caagtggcac
     4501 tggccctgcg cagcgatccg ttgctccagg ccggcacgcg gatcgcggtg gagacgacct
     4561 tcagcgagga tccgctggtg ccgtaccagg cgtggaccga catcatcacc acgatgttca
     4621 ccgaggcccg ggacaacggt gaattactgc ccggggtggc accggaccgg gcggccgagt
     4681 tcttcgtcgc cgcctacatg ggcgtgcagt tgttctcgcg cgccgcgacc aaccgcgccg
     4741 atctcccgga gcgggtcacg gccctgtgga agcacacact tccgggcctc gcctcgcccg
     4801 gtgcgctgag ccatctggat ccgcacggcc gctgcgtcaa ggtctcggtc tgacgttccg
     4861 ggccaccgcc ctggcggagc cgacggctcc ttcccggctc tggaaaacaa accacatggt
     4921 ccgtatctta gtgttttggg ccgtgaccaa gcctcagccc gtcagggcgc tgagagccgc
     4981 accacagaaa ggaaccacct atggccagcg ccacgctcac gccgtacttg cgcgatcagc
     5041 gccacgacat cggaggcggg tccgccgacg gaatccgacc gtcgtcgctc accacgaccg
     5101 tgccgcgcga gtacgtccac cgcaacgcgt tgtccgaggt gttcctgacg aactggcggc
     5161 gtggtggcgc ggatacctgg gtggtcagcg cccagtggcc gcgcgcccac accttctacg
     5221 ggccgctcca cgggatgcac gacccgatcc tgctgataga gacggtgcgg caggccggca
     5281 tattactgag ccatgtcgcg caccgggtgc cgctcgacca cccgatcatc tggcagcggg
     5341 tccgctacga cctcgcgccg caggcactgc gcaccgcgca gcaccccgcc gaggtcgaac
     5401 tgcacatcac cgaccgcgac atggtccacc gcggcaagcg gctggtcagc gccctccagg
     5461 agttccggat ccggtgcgac ggcgccgagc tggcctccgc gacgctcgac tactcctgtc
     5521 acagcccggc cgtctaccgc cggctgcgcg gcgagtacgg cgatctggcg ttcgccaact
     5581 cccgtacgct gccgctgccc ggggccatga gcccgcagct ggtcgcccgg gaacacgacc
     5641 gcgatgtggt gctctccccc accgaccgcc ccgaccgctg gcagctgcgg gtggacacct
     5701 cgcacccggt tctcttcgac caccccgtgg accacgcccc cggcatgctg atggtcgagg
     5761 ccgcccggca ggccgcgcag gccgccacgc ccggcgtcac cctgccggtc tcgatggagt
     5821 gctccttctc gcgctacgcc gaactcgacg cgccgatctg ggtacaggcg cggaccaccg
     5881 gccggggcga cgacgaccgc cggcaggtcg aggtcaccgt cgaacagcac ggccggccgg
     5941 tcgtctcggc gcaggtcagc tgcgtaccga caccgccctg accgaccccc cggccgacca
     6001 ccgcaccggc ccgccggacc cctgaccggc ctctccgccg gcccgtcggc gtccttgcgg
     6061 accggccccg cgaacggccc cgccccgctg cccgtgcctc cccgtcaccg cagcgtgagg
     6121 cggggcttcg gcgcgtccag gcgtccggtg ggcggagtgc ggctgcccgc ctggtagggg
     6181 agcggccagg gcgcgcccgg tccggcgtac ccctgctcgg cggcggcgtg cagcgtccag
     6241 tgcgggtcgt acagatgcgg tctggccagc gcgcacaggt ccgtacgtcc ggccagtacc
     6301 agggagttga cgtcgtccca ggaggagatg gcgccgaccg cgatgaccgg cacgccgagg
     6361 gtgttgcgga tacggtcggc gaacggggtc tggtaggagc ggccgaagtc ggggcgctcg
     6421 tcgggcacca cctggccggt ggacacatcg atggcgtcgg cgccgtgctc ggcgaaggcc
     6481 cgcgccatcg ccaccgcgtc ctccgcggtg gtgccgccgt ccgcccagtc ggtcgcggag
     6541 atccggaccg tcatcggacg cccggccggc cagacggccc ggaccgcgtc gaagacctcc
     6601 agcgggaagc gcagccggcc ggtgaggtcg ccgccgtagg cgtcggtgcg gtggttggtg
     6661 agcggtgaga ggaagccgga gagcagatag ccgtgggcgc agtggagttc gagcaggtcg
     6721 aagccggctc gggcggcgga ctcggcggcg cggacgaact gtgcgcggag ggtgccgagt
     6781 tcgggttcgg tcagggcgtg cggggtctgg ttgacgcccg ggcggtaggg cagcgcggag
     6841 ggcgccacga ggggccagtt gccgtccggg agcggctggt cgatgccgtc ccacatcagc
     6901 cgggtggagc ccttgcggcc ggagtggccc agctggacgc cgatggcggt gccgggggcg
     6961 gaggtgtgca cgaagtcggt gacccggcgc caggccgtct cgtgctccgg cgcgtacagc
     7021 cccgtacagc cgggcgtgat ccgcccctcg gggctgacgc acaccatctc cgtcatcacc
     7081 agtccggccc cgcccagcgc gcgggcgccg aggtggacga ggtggaagtc gccgggggtg
     7141 ccgttgttgt ccgcggcgga gtacatgtcc atcggggaga ccacgacccg gttgcgcagg
     7201 gtcagctcac ccagccggaa gggcaggaac atcgggggtg tgccgggcgg ggtgcccgcc
     7261 tcgtcctcga cggccgcgac gaatccgggg tcgcgcagcc gcaggttgtc gtgggtgacg
     7321 cggcggctgc gggtgaggag gttgaaggcg aactggtgcg gcggctggtc gagataggtg
     7381 gccagctcct cgaaccaggc gaggctggcg cgggccgcgc gctgggtgga cgcgacgacg
     7441 gggcggcgct cctcctcgta cgcggccagc gcggaggccg tgtcgggctg ctcctcaaga
     7501 caggccgcca gcgccagggc gtcctcgacg gcgagcttgg tgccggagcc gatggagaag
     7561 tgcgcggtgt gcgcggcatc gccgagcagc acggtgttgc cgtgcgacca gcggtcgttg
     7621 acgaccgtgc ggaaggcgat ccagctggag ttgttgccgc gcagcgaccg gccgcgcagg
     7681 gcgtcggtga agaccttggc gcaccactcg gccgactcgc gctcatcgca gcggtcgagc
     7741 ccggccgacc gccagacctc ctcgcgcatc tcgacgatga ccgtgctggc gccgtccgcc
     7801 gggccctccg gccgggcgaa ggggtaggcg tgcagctgga ccacgccgtg ctcggtctcg
     7861 gcgatctcga agcggaaggc gtcgaaggcg aagtcggcgg cgagccagat gtagcggcag
     7921 cggtgggtgg tcagctgcgg gtggaaggcg tcggcgtgcg cggtgcgggt ggcgctgtgc
     7981 accccgtcgg cggcgatcac caggtcgtac gcacgggcca gttcggcggc gggcggggcc
     8041 tcggtgcgga agcgcagctc gatgccgaga tcgcggcagc gccggtggag tacggccagc
     8101 agccggcgcc ggccgagcgc cgcgaagccg tgtccgccgg aggtcagggt gcggccccgg
     8161 tggacgatgt cgatgtcgtc ccagcggacg aactccgcct ggagggcgcg gtagacctcc
     8221 gggtcggcgt gctcgatgcc accgagggtc tcgtcggaga gcacgacgcc gaacccgaag
     8281 gtgtcctcgg gtgcgttgcg ctcccatacg gtgatctcgc gggtggggtc gaggcgcttg
     8341 agcagcaccg cggcgtacag cccgcccggc ccgccgccga tgacggcgac ccgcatggct
     8401 atttcccctg ccacttgggg gcccgcttct ccgtgaaggc ggcgtggaat tccgcgtagt
     8461 cgtcgctgtt catcagcagc gcctgggtgg cggcgtccat ctccacggcg gcggccagcg
     8521 gcatatcgag ctcggcggtg agcagcgcct tggtctgggc gtgcgccatc gcgggccctt
     8581 cggcgagccg ccgggcgagc gcagcggccg cctcgtcgac ccggccctcc tcggccaact
     8641 cgctgatcag gccgaggcgt tcggcctccg gtgcccggac ggtgtcgccg agcatcagca
     8701 gccgggtggc gtggccgagg ccgatgacgc ggggcaggag ataggccgcg cccatgtcgc
     8761 cgccggagag tccgacccgg gtgaagagga aggcgaaccg ggcggacggg tcggcgaccc
     8821 ggaagtccgc ggccagcgcg agcaccgcgc ccgcccccgc cgcgaccccg tgcaccgccg
     8881 cgatcaccgg gaacgggcac tcccgcaccg cccggacgac ctgcccggtc atccggttga
     8941 agtccagcag ttgggcggtg tccatgccca gggtggcgcc gatgatctcc tcgacgtcgc
     9001 cgccggagca gaatccgcgc ccctcccccg cgagcaccag ggcgcgtacg gaacgctccc
     9061 tggacagctc ggcgagcagg tcgcggaggt cggcgtaggc ctcgaaggtg agcgcgttga
     9121 gcttgtccgg gcgcgccagg gtgacggtgg cgaccccgtc ctgccgcgtc acgcgaaggt
     9181 gctgccaggt ctcggtactg cgggcggatc ctgcgaacgg gctcatcggg ctgcctgccc
     9241 cctttggccg ggtcggccgg tggatgctgt tccggatgcc tctcgaagtt atcactcctt
     9301 catgactgtc gtcatgagaa cgcgatacga cacggccgac gacgacggcc cgccacccgc
     9361 cggggccgcc agggtggacc cgccgaaggt cccggccgcc gcatgacgta ggtcatccga
     9421 tccccggccg ctcgcccctg ctcccccgcc tccgcgcttt ctacggtgga ggggcccgat
     9481 ccggtctcag gccgccgacc cggcctttcg cgaacggacg caccgcgcat gctggaacca
     9541 cccacccctg cgacctggcg catcgcgctg ccgcgcggcg cgaccgccgt ccccatcgcc
     9601 cgtgcgatgg tccggaccgc catgcaggat ctccgggtga cggccgaccg gaccaccgct
     9661 caactactga ccgcggagct ggtcaccaac gtgctcaagc acacctgcgg tgccgacccc
     9721 atcgagctgg tggtggaaca gcggccgacg ggctgccgga tcgaggtaca cgacgacagc
     9781 ccgctgctcg tgaagggcct gggcgatccg gtcccccttg gagaaccggg ggcgggggtg
     9841 gtgggagcgc cgggcggtgg cgcgccggat ggcccggggg tggacccgcc cggcggcata
     9901 tcggccggcc cgtcggccgg gccgtcaccc ggccggtcgt cggacggcga ggtgccgact
     9961 cagcgggcgg aggaggaggc cgggcggtcc gcagacggcg ccgagacggg cgatggcggg
    10021 gaggggcagg agctggacgg ggaggggcag gggcccgacg aggacaacgg gcatgccgga
    10081 gtcgatggcg acacctacgc cgacggctcc ccggactccc gttccgcgga cgacgacgat
    10141 gccgacgcca cggccgccca cgggaccgac accggccccg acgccctctc ccccggcccg
    10201 gcccgcggtg accacgacgg tcgtggtctg ctgctgatcc ggtccctcag cgcggcctcc
    10261 ggctgtcgcc cgacgccgcg cggcaaggcc gtctggttca cccttcccgg ggtgcggcgc
    10321 ccgcgccgac gctgacggcg gtcgcgccct cgccggcacc ggccgccggg tcctgcgccg
    10381 ccgccgcgag ccgcgagcga cggcgcccgt agcacgcgta gaccagcgca ccggccacca
    10441 ggaacccggc gaactggagc caggtcgccg gaccggtgcc gtacatcaga tagaggcaga
    10501 aggcgatgcc gagcgccggg ctgaccggga agagcgggac acggaagcgg cgcggcagat
    10561 cggggcgggt acggcggagc accatcacgc tgaggttgac caccgccatg accgccagcg
    10621 tcccgatggt cgacaggttc atcaccgcgt ccaacgggac gaccgcggcc ggcacggcga
    10681 aaacgaccgc gacgatccag gtgttggcga cgggggtgga cgtccggggc gagacccgct
    10741 cgaagatccg cgggaccagg ccgtcccgcg ccatcgacat caggatgcgg gtctggccgt
    10801 acatgaccgc gaggacgacc gaggcgatgg cgacgacggc gccgaacgcg atcaccccgc
    10861 cgccgagcgt cgagtgggtg acgtggttga cgatcagcga gagcgcggcg ggccgcccgc
    10921 cgacctcgcc ggcggagagc gcgccgatgg cggagaccgc gaccaggcag tacaggacgg
    10981 t
//
