LOCUS       NZ_BBNO01000008        11330 bp    DNA     linear   CON 19-AUG-2024
DEFINITION  Streptomyces lydicamycinicus strain TP-A0598, whole genome shotgun
            sequence.
ACCESSION   NZ_BBNO01000008
VERSION     NZ_BBNO01000008.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces lydicamycinicus
  ORGANISM  Streptomyces lydicamycinicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Komaki,H., Hosoyama,A., Igarashi,Y. and Tamura,T.
  TITLE     Streptomyces lydicamycinicus sp. nov. and Its Secondary Metabolite
            Biosynthetic Gene Clusters for Polyketide and Nonribosomal Peptide
            Compounds
  JOURNAL   Microorganisms 8 (3), 370 (2020)
   PUBMED   32155704
  REMARK    DOI:10.3390/microorganisms8030370 Publication Status: Online-Only
REFERENCE   2
  AUTHORS   Komaki,H., Ichikawa,N., Hosoyama,A., Fujita,N. and Igarashi,Y.
  TITLE     Draft genome sequence of marine-derived Streptomyces sp. TP-A0598, a
            producer of anti-MRSA antibiotic lydicamycins
  JOURNAL   Stand Genomic Sci 10, 58 (2015)
   PUBMED   26380643
  REMARK    DOI:10.1186/s40793-015-0046-5 Publication Status: Online-Only
REFERENCE   3  (bases 1 to 724454)
  AUTHORS   Hosoyama,A., Kimura,A., Ohji,S., Ichikawa,N., Yamazoe,A. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2014) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Newbler v. 2.6
            Genome Coverage                   :: 93x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina HiSeq 1000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000829715.2-RS_2024_08_19
            Annotation Date                   :: 08/19/2024 02:08:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,323
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,092
            CDSs (with protein)               :: 7,092
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 4, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 54 of 145
            Pseudo Genes (incomplete)         :: 123 of 145
            Pseudo Genes (internal stop)      :: 9 of 145
            Pseudo Genes (multiple problems)  :: 39 of 145
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:07:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 425285
            Orig. end                         :: 436615
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBNO01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11330
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11330
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11330
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6330](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6330
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            682..2049
                     /locus_tag="TU31_RS28160"
                     /old_locus_tag="TPA0598_08_03700"
     CDS             682..2049
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602440.1"
                     /locus_tag="TU31_RS28160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_03700"
                     /product="alkaline phosphatase PhoX"
                     /protein_id="WP_042159566.1"
                     /transl_table=11
                     /translation="MSATRRQILARTGALGAGIAFSGSLTELFTGTATAQSMGQRGYGP
                     LVPDPDGLLDLPRGFRYRVLSREGDPLRSGEGKVPSNCDGMAAFPGHRGGTHLVRNHEN
                     RTNSRQAVPRVRDLTYDPGGEGGCTALALDGDNKVLSERVAIAGTSTNCAGGPTPWNTW
                     LTCEETEFRAGEENYTKDHGFIFEVDPYDPRRTGAVPLTAMGRFQHEAIAVDPGTGIVY
                     ETEDAFEKPFGLFYRFLPEKPMGGRGSLRAGGRLQALRVPGVPDLSTIQRPGTTFDHVT
                     WVDVPDPLARETPIRFQDFGPQGITHAQKLEGCYWGGSCVYFISSFAHREAGSAADHYG
                     QVWKYDPKKRRLTQVVVFGPDSDLQLPGESPDNICLAPSGGLMVCEDGEGAQHVFGLTR
                     RGEVYAMARGRQNIGTPQKPEWGEFAGVTFSPDHRTMYVNCYTPGTTFAVTGPWHH"
     gene            complement(2223..2891)
                     /locus_tag="TU31_RS28165"
                     /old_locus_tag="TPA0598_08_03710"
     CDS             complement(2223..2891)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088894.1"
                     /locus_tag="TU31_RS28165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_03710"
                     /product="hypothetical protein"
                     /protein_id="WP_042159568.1"
                     /transl_table=11
                     /translation="MIALLRYQTALLIGSHRWLPPVILYAAWLAIGVQSGGPILDAFGY
                     AAGGLLPAAVWLTRVCVTNEPPAARNCAAAAAGPGRVHLAAVLTAAGTGLLLALLGTAV
                     VVLLSDPRSTDLQVAVPVPPAAFAGLLAALVCLLMGIAIGALSNWPVLRSTGWSIPTGL
                     LAALLLLVLGASPANAAVSGLVTGSVHGTISTPWLPLAATGLLAAAATAAACALSSRRG
                     "
     gene            complement(2888..3808)
                     /locus_tag="TU31_RS28170"
                     /old_locus_tag="TPA0598_08_03720"
     CDS             complement(2888..3808)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602442.1"
                     /locus_tag="TU31_RS28170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_03720"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_042159571.1"
                     /transl_table=11
                     /translation="MRLDGVGRRYGVRGPWVLREVDLEVPARALLRVEGTNGSGKSTLL
                     RLLAGIDRPSTGRITGRPRTAYVPERFPVALPFTAVGYLTHLGRIHGLPGPEATRRATE
                     WLERFGAAGHARTPLAELSKGTSQKVAVAQALLAEPDLLVLDEAWTGLDGGAREVLDRA
                     VAERVADGGTVVFVDHDPQRLAGAADARYRVADGRLLTHEESGPTAGAKAAAGPRVRIE
                     AEGAARSLPPTGAPPLPGGPEIAPGPDGTIRLTVPAAHSDALLRALLTARPPWHIRSVT
                     AVPLPHEAAPAAADDSPAPDEAPLP"
     gene            4035..4784
                     /locus_tag="TU31_RS28175"
                     /old_locus_tag="TPA0598_08_03730"
     CDS             4035..4784
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602443.1"
                     /locus_tag="TU31_RS28175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_03730"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_042159572.1"
                     /transl_table=11
                     /translation="MTSTETAGPSGNSADSPFEHAETDRDAAPQFVLPLVVRIEREAPP
                     ARTDALETAARAVLVLLADERSAGDGEWAAAVRDWQDARIRKVVRRARRAEWRRAAALP
                     GITVTGHGAEVRVFPPVPLDGWPKDLARLQVSGTELDDPQPPGAPADGPVLWFNPELEM
                     SAGKEMAQAGHGAQLAWWELSEEERAAWRAAGFPLSVRSARRVEWDALTRSGLPVVRDA
                     GFTEIAPGSCTVVADHPALRRALSAEG"
     gene            5001..6330
                     /locus_tag="TU31_RS37330"
                     /pseudo=""
     CDS             5001..6330
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564414.1"
                     /locus_tag="TU31_RS37330"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 2.9e-95, bitscore: 313.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIAAEIAELPGVDWVEVVAENICPGHLPYALEE
                     LRERGTTVIPHGVSLGLGGADRPDPGRLTALAERAEALGAPLVTEHIAFVRAGGPLTAS
                     PRMEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALINWPGEELTEGQFLA
                     ELVERTGVRLLIDVANLHTNHINRGEDPQAALDELPLDALAYVHVAGGVERDGVWHDSH
                     AHPVTRPVLDVLAALCDRTTPPGVLLERDDVFPEGGELAGELDAIRTVLKEARHDRAA"
     gene            6336..7316
                     /locus_tag="TU31_RS28190"
                     /old_locus_tag="TPA0598_08_03760"
     CDS             6336..7316
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175676.1"
                     /locus_tag="TU31_RS28190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_03760"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_078886045.1"
                     /transl_table=11
                     /translation="MSYPQAVYAGIAASSLLLILGTVSVRLRTPSVRRTDALAHDVWEM
                     AFLAGGPGRVVDAALAGMHEDGRLAVGGPGVVTVRQALARDAVEAAVLDAIARTPGGAL
                     AALRGTAMRSPAVQAVGDGLAARGLLRRPQLGRGWRRWAGAQTAVCGVLFFVGLLLSVA
                     GSGGDDGFGVPPVVTTAPAVIGGLVVASLCRRAFTRRITKAGSFALHTAKVAHAPAGVG
                     GVGQPAGLVVALGGTALIADELLRQQFEEAQRAAAGSGSASSGSSASDTSGGGSGSDGG
                     SWCGSSDGGSGCGSGGSSCGGGASCGGGSSCGGGSSCGGGGCGGG"
     misc_feature    6341..6357
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.62"
                     /tool="antismash"
     misc_feature    complement(6342..6357)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 21.07"
                     /tool="antismash"
     misc_feature    complement(7366..7376)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 17.67"
                     /tool="antismash"
     gene            7390..8190
                     /locus_tag="TU31_RS28195"
                     /old_locus_tag="TPA0598_08_03770"
     CDS             7390..8190
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088889.1"
                     /locus_tag="TU31_RS28195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_03770"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_042159576.1"
                     /transl_table=11
                     /translation="MWLLFFLVACSAAVLSCALLCRAAVAAARATPGPAPRPAPETVPD
                     AAAARRGPGLTLYEMAYLSGGPHRLADVVLVLMAQQRRLLLADTGWATVVDPVGKDAVE
                     RAALTAIGPEGQCRIPAIRDALVGDDAVRQVADRLVTDGLAVPAAVRPGVGSAVRLVQG
                     AALLVLLSAAASYWMAPAGADTGLLPAWFSLPLILTLGTWAVARFELHPYSDWATTAGE
                     RQLPGVSHSPAAPATPASLVTLALRGPAALTDPALRAALHSSGA"
     gene            8275..9963
                     /locus_tag="TU31_RS28200"
                     /old_locus_tag="TPA0598_08_03780"
     CDS             8275..9963
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (92-508). E-value:
                     5.5e-18. Score: 57.4. Matches aSDomain:
                     nrpspksdomains_TU31_RS28200_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602445.1"
                     /locus_tag="TU31_RS28200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_03780"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_042159577.1"
                     /sec_met_domain="PF00561 (E-value: 1.3e-25, bitscore: 85.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGTIGSLLVTALATAPAGGATGAAAAPPAPVPHRIEFGR
                     CAAVEHLPSTVECGRLTVPLDYARPDGKKIHLTVSRIRATARGERQGALVFNPGGPGAS
                     SMEFPLYGGLPKWRKVARAYDFVGYAPRGVGRSAPLSCQNPKEFAKAPTDSRMHPDAAF
                     KRQKRAEAREYARGCVRRAGADLPYYNSVNNARDLDMLRAALGEKKLTFMGASYGTYFG
                     AVYATLFPGHVRRMIFDSVVNPAPRKIWYQNNLDQDVAFERRWGDWLGWVARHHAHYHL
                     GTTKKAVQQAYNKAAGKLRRTPLNGKIGAGQLQSAFLKTGYNDAFWPMRAEALAGYLHG
                     NPKPLIAQALPRDTGAAADENSNAVYTAVECNDAPWPHDWRTWNRDNTEIARIAPFETW
                     DNAWMNLPCAYWPDRPASAAAELDDAVRRADHPAAGRDLADVAGHVARIRIDDVLHGRR
                     GPLDVHTEPGALPPVLLLAAERDAATPYRGALELQRRLPGSSLVTENGAGTHGVAFNDN
                     ACVTKYAEAYLLHGKIPAHKVYCAPRAEPLPGQQATRAQHALPGK"
     aSDomain        8551..9798
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TU31_RS28200_Abhydrolase_1.1"
                     /evalue="5.50E-18"
                     /label="TU31_RS28200_Abhydrolase_1.1"
                     /locus_tag="TU31_RS28200"
                     /protein_end="508"
                     /protein_start="92"
                     /score="57.4"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASSMEFPLYGGLPKWRKVARAYDFVGYAPRGVGRS
                     APLSCQNPKEFAKAPTDSRMHPDAAFKRQKRAEAREYARGCVRRAGADLPYYNSVNNAR
                     DLDMLRAALGEKKLTFMGASYGTYFGAVYATLFPGHVRRMIFDSVVNPAPRKIWYQNNL
                     DQDVAFERRWGDWLGWVARHHAHYHLGTTKKAVQQAYNKAAGKLRRTPLNGKIGAGQLQ
                     SAFLKTGYNDAFWPMRAEALAGYLHGNPKPLIAQALPRDTGAAADENSNAVYTAVECND
                     APWPHDWRTWNRDNTEIARIAPFETWDNAWMNLPCAYWPDRPASAAAELDDAVRRADHP
                     AAGRDLADVAGHVARIRIDDVLHGRRGPLDVHTEPGALPPVLLLAAERDAATPYRGALE
                     LQRRLPGSSLVTENGAG"
     gene            complement(10048..10809)
                     /gene="hemQ"
                     /locus_tag="TU31_RS28205"
                     /old_locus_tag="TPA0598_08_03790"
     CDS             complement(10048..10809)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536947.1"
                     /locus_tag="TU31_RS28205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_08_03790"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_370682770.1"
                     /transl_table=11
                     /translation="MDHMTDASTPASAPQTPAAPEKVPHAGKKAKDLNEVIRYTLWSVF
                     KLRDVLPEDRSGYADEVEELFAQLAAKDVVVRGTYDVSGLRADADLMIWWHSESSDALQ
                     EAYNLFRRTRLGRALEPVWSNMALHRPAEFNKSHIPAFLADEQPRAYVSVYPFVRSYEW
                     YLLPDEDRRRMLADHGKMARGFPDVRANTVPSFSLGDYEWVLAFEADELYRIVDLMRHL
                     RGSEARMHVREEIPFYTGRRKPVAELVAGLA"
ORIGIN
        1 caccctgctc ggcaagctgg tggacgccgg ggtggcgctg gccgccacct ccaacaccct
       61 ccccggcaag ctcggcgagg gccggttcgc ggccgccgac ttcctgcgcg agatccaggg
      121 gctgagcgcc cacttccgcc cgctgcgcat cgacggcgag gactaccgcc accgcggtct
      181 gcccgaggcc cccgccccgt acgacgacga gaccgtcacc cgcaccgcgt accgcacccc
      241 cggcgcctcg ctcgacgact tcccctccct gctggaccat ctgtccaagg tgcaccccag
      301 ccgctacggc gccatgtgcg acggcatcga ggcggtctgc ctcaccgatg tccaggcggt
      361 gcccgaccag tccacggcgc tgcggctggt cgtcctcgcg gaccggctct acgaccgtga
      421 ggtgccggtg gtgacctccg gcaaggcctt cgacgagctc ttcagcgacg agatgctgcg
      481 gggcggctac cgcaagaagt actaccgggc gatctcccgg ctgacggcgc tggcccgcga
      541 cgcgggcgcc ctggtggccg acgcgcggta ggcggaacag cggcaacacc cggttggtgc
      601 ccgtcggctg gttcccggcg gcccggccgc gtggtacaca cgagcgggtc acaccacctg
      661 tccgccaaca gggagttgcc catgtccgcg acacgacgtc agatcctggc acgcaccggt
      721 gcgctgggag cggggatcgc cttctccgga agcctcacgg agctcttcac gggaaccgcg
      781 accgctcaga gcatgggaca gcgcggctac ggcccgctgg tgccggaccc ggacggtctg
      841 ctcgatctcc cccggggctt ccgctaccgg gtcctgtccc gggagggcga cccgctgcgc
      901 tcgggagagg gcaaggtgcc cagcaactgt gacgggatgg ccgccttccc cggccaccgt
      961 ggcggcaccc acctggtccg caaccacgag aaccgtacga actcccgcca ggccgtcccc
     1021 cgggtccgcg atctgaccta cgaccccggt ggcgagggcg gctgtacggc cctcgcactc
     1081 gacggcgaca acaaggtgct ctcggagcgg gtcgccatcg ccggcacctc caccaactgc
     1141 gcgggtggtc ccaccccctg gaacacctgg ctgacctgcg aggagaccga gttccgggcc
     1201 ggtgaggaga actacaccaa ggaccacggc ttcatcttcg aggtcgatcc ctacgacccg
     1261 cggcgcaccg gagccgtccc gctgaccgcg atgggccgtt tccagcacga ggcgatcgcc
     1321 gtggaccccg gcaccggcat cgtctacgag accgaggacg ccttcgagaa gccgttcggc
     1381 ctcttctacc gctttctgcc cgagaagccg atgggcggcc gcggttcgct ccgggccggc
     1441 ggccgcctcc aggcgctgcg cgtccccggt gtgcccgacc tctccaccat ccagcggccc
     1501 ggcaccacct tcgaccacgt cacctgggtc gatgtgcccg acccgctggc ccgtgagacc
     1561 ccgatccgct tccaggactt cggcccccag ggcatcaccc acgcccagaa actggagggc
     1621 tgctactggg gcggctcctg cgtctacttc atctccagct tcgcccaccg ggaggccggt
     1681 tcggcggcgg accactacgg ccaggtgtgg aagtacgacc cgaagaagcg gcggctgacc
     1741 caggtcgtcg tcttcggccc ggacagcgac ctccaactgc ccggcgaatc ccccgacaac
     1801 atctgcctcg cgcccagcgg cggcctgatg gtctgcgagg acggcgaggg cgcccagcac
     1861 gtcttcggcc tcacccggcg cggcgaggtc tatgcgatgg cccgcggccg gcagaacatc
     1921 ggcaccccgc agaaaccgga gtggggcgag ttcgccggcg tgacgttctc gcccgaccac
     1981 cgcacgatgt acgtcaactg ctatacgccg ggaacgacgt tcgcggtgac ggggccttgg
     2041 catcactgag gcgggggcgg gtccctggcg ccactgatgc tgcgccgggg gcccggtgcc
     2101 actgacgctg cggcggggac cactcccccc cacccccgaa cccacccccg ccccctcccc
     2161 cgaaggggga ggagggggag ggggcggggg agagccggcg ccccttcccg gaggcttccc
     2221 cttcagcccc gccgtgagct gagggcgcac gcggccgccg tggcggcggc cgcgaggagc
     2281 ccggtggcgg cgagcggcag ccaaggggtg ctgatcgtcc cgtggacgga gccggtgacc
     2341 aggcccgata cggcggcgtt ggcgggcgaa gcgcccagga ccaacagcag cagggcggcg
     2401 agcagacccg tcgggatgct ccagccggtg ctgcgcagca ccggccagtt gctcagcgcg
     2461 ccgatggcga tgcccatgag cagacacacc agcgcggcga gcagcccggc gaacgccgcg
     2521 ggcgggaccg gaacggccac ctgaaggtcg gtggagcgcg ggtccgacag cagcacgacc
     2581 acggccgtgc cgagcagcgc cagcagcagc ccggtgccgg ccgcggtcag tacagcggcg
     2641 aggtgtaccc ggccgggccc ggcggcggcc gcggcgcaat tgcgggcggc cggcggctcg
     2701 ttggtgacac agacccgggt cagccatacg gcggccggca gcagcccgcc ggcggcgtag
     2761 ccgaacgcgt cgaggatcgg cccgccgctc tggacgccga tggccagcca ggccgcgtag
     2821 aggatgaccg gcggcagcca gcggtgcgag ccgatgagca gtgccgtctg ataacggagg
     2881 agagcgatca cgggaggggg gcctcatcgg gtgcggggga atcgtcggcg gcggcggggg
     2941 cggcctcgtg cggcagcggg acggcggtga ccgagcggat gtgccacggc ggacgggcgg
     3001 tgagcagggc gcgcagcagg gcgtcggagt gggcggccgg caccgtcagc cggatcgtcc
     3061 cgtccggtcc gggcgcgatc tccggcccgc cgggcagcgg cggcgctccg gtgggcggca
     3121 gactccgcgc tgcgccctcg gcctcgatgc gcacacgggg accggccgcg gccttcgcac
     3181 ccgccgtcgg accgctctcc tcgtgggtca gcagccgtcc gtcggcgacc cggtagcggg
     3241 cgtcggcggc gcccgccagc cgttgcgggt cgtggtcgac gaacacgacg gtgccgccgt
     3301 ccgcgacccg ttcggcgacc gcccggtcga gcacctcgcg cgcgccgccg tccagaccgg
     3361 tccaggcctc gtcgaggacc aggaggtcgg gctcggcgag cagcgcctgg gcgaccgcga
     3421 ccttctggct cgtccccttg gacagctctg ccagcggggt ccgggcatgc ccggccgccc
     3481 cgaagcgttc cagccactcg gtggcgcggc gggtcgcttc gggtccgggc agcccgtgga
     3541 tgcggccgag gtgggtgagg tagccgacgg cggtgaaggg gagggcgacc gggaagcgtt
     3601 cgggcacata ggcggtgcgc gggcggccgg tgatccggcc ggtgctgggc cggtcgatcc
     3661 cggcgagcag ccgcagcagg gtggatttcc cgctgccgtt ggtcccctcg acgcgcagca
     3721 gcgcacgggc gggcacttcg aggtccacct cccgcagcac ccaggggccg cgcaccccgt
     3781 accgccgccc caccccgtcc agcctcatac gtcccggcac ctccgttgtc tcctggcggc
     3841 cccgccgcac caagtccccg acggtagcca aggcggccgc ccggtgcctg cccgggggcg
     3901 cgttcagcgc cggacggtac gtgcctcaac ctcgcgccgg gcggacggag ttggccccgg
     3961 ccgtggcccg gcgtgggccg gggagccgga tacgggccgg gggcgctgtc ggcggcggct
     4021 ggcagactgg gcaggtgacc agcaccgaga ccgccgggcc gtccggcaac agcgccgaca
     4081 gccccttcga gcacgcagag accgaccggg acgcggcgcc gcagttcgtg ctgccgctcg
     4141 tcgtccggat cgagcgggag gccccgccgg cgcgtacgga cgcgctggag acggccgcgc
     4201 gggcggtgct ggtgctgctg gcggacgagc ggtcggccgg ggacggcgag tgggccgcgg
     4261 cggtgcggga ctggcaggac gcccggatcc ggaaggtggt gcggcgggcg cgccgcgccg
     4321 agtggcggcg ggcggcggcg ctgccgggca tcacggtcac cggccacggg gccgaggtgc
     4381 gggtcttccc gccggtgccg ctggacggct ggccgaagga cctggcgcgc ctccaggtct
     4441 cgggcacgga gctggacgat ccgcagccgc ccggggcgcc cgccgacggc ccggtgctgt
     4501 ggttcaaccc cgagctggag atgtcggcgg gcaaggagat ggcgcaggcc gggcacggcg
     4561 cgcagttggc gtggtgggag ctgtccgagg aggagcgggc ggcctggcgg gcggccgggt
     4621 tcccgctgtc ggtgcggtcc gccaggcgcg tggagtggga cgcgctcacc cgcagcggtc
     4681 tgcccgtggt gcgggacgcc ggattcaccg agatcgcgcc gggttcctgc acggtcgtgg
     4741 ccgaccaccc cgcgctgcgg agggccctgt cggccgaggg ctgagccggc aggacggggc
     4801 tcgcgcccgg cccgatcagg ccaccgtcgg cgccgcccgc gccggtcacc gtcgtagggc
     4861 agaggtgggc ccgcagcggc gccgcgcagc accgccacgg cgccggtgaa gctcaaatca
     4921 acctctgaac tcggccggtg cagggttcgg ccgggcctca cgcggcatga gtacgacacc
     4981 acggagcgag agggggagcc atggagcgcc tgggtacggg aatcggctgg cggccggaaa
     5041 tcgccgcgga gatcgcggag ctgcccgggg tggactgggt cgaggtggtc gcggagaaca
     5101 tctgcccggg ccatctgccg tacgccctgg aggagttgcg cgagcgcggc acgaccgtga
     5161 tcccgcacgg ggtctcgctc ggcctcgggg gtgccgaccg tccggacccc ggccggctga
     5221 ccgccctggc cgagcgcgcc gaggcgctcg gcgcaccgct ggtcaccgag cacatcgcct
     5281 tcgtgcgggc gggcggcccg ctcaccgcgt cgccccgtat ggaggccggg catctgctgc
     5341 cggtaccgag gacccgggac gcgctggacg tgctgtgcga gaacgtacgg atcgcccagg
     5401 acgccctgcc ggtgccgctg gcgctggaga acatcgccgc gctcatcaac tggccgggcg
     5461 aggagctgac cgaggggcag ttcctggccg agctcgtgga acggaccggg gtgcggctgc
     5521 tgatcgatgt cgccaacctc cacacgaacc acatcaaccg gggcgaggac ccccaggcag
     5581 ccctcgacga actgccgctg gacgccctcg cctacgtcca tgtcgcgggc ggggtggagc
     5641 gcgacggggt gtggcacgac agccacgccc acccggtgac caggcccgtg ctggacgtgc
     5701 tcgccgcgct gtgcgaccgg accaccccgc ccggggtgct gctggagcgg gacgacgtct
     5761 tccccgaagg cggggaactg gccggcgaac tcgatgcgat acgcaccgtg ctgaaggagg
     5821 cccgccatga ccgggcggct tgaggcggtg ccggacatcg cgtcggtaac ggacgccact
     5881 gacgaggcgg ccaccgcccc cgaccccggc acccacgccg cccgccagcg gctggcgctg
     5941 gcgcagaccg cgctgctgtc cgcgctggtc gccgggatgc ccgcccccga gggcttcgac
     6001 cgcacccggc tgcgggtgca gagccgggcg ctggccgcca agcgggccga tgtggtcgcc
     6061 aaggtcgcgc ccgagctgcc ggagatcctc ggcgccggct accgggccgc cttcgcggac
     6121 catgcccgga accgtccgat gagcggcggc taccgccagg acgccctgga cttcgcggcg
     6181 catctgctgg cccaggggcg gcccgaagat gccgccgcgc ggcgggagtt gacgcgctgg
     6241 tggcgggagc gtgcgggtcc gaagccgccg tccgcacggc ccgcggcccg gctggcgcgc
     6301 gctctgcggc tcttccggcg acgggggtga ccgcgatgtc ctacccccag gcggtgtacg
     6361 cggggatcgc cgcctcgtcg ctgctgctga tcctcggcac cgtatccgtg cggctgcgca
     6421 ccccgtccgt ccggcggacc gacgccctgg cccacgacgt gtgggagatg gcgttcctgg
     6481 ccggtggccc cgggcgggtg gtggacgccg cactcgccgg gatgcacgag gacggccggc
     6541 tggccgtggg cggccccgga gtggtgaccg tgcgccaggc gctcgcgcgg gacgccgtcg
     6601 aggcggccgt gctggacgcg atcgcccgga cgcccggcgg ggcgctcgcc gcgctgcggg
     6661 gcacggcgat gcgcagcccg gcggtgcagg ccgtcggcga cgggctggcg gcccgcgggc
     6721 tgctgcgccg gccgcagctc ggccggggct ggcgccggtg ggcgggtgcc cagacggcgg
     6781 tgtgcggggt gctcttcttc gtgggtctgc tgctctcggt cgccgggtcc ggcggggacg
     6841 acgggttcgg ggtgccgccg gtcgtcacga ccgcgcccgc ggtgatcggt gggctggtcg
     6901 tggcgtcgct gtgccggagg gcgttcaccc gtcggatcac caaggcgggg tcgttcgccc
     6961 tgcacaccgc gaaggtggcg cacgcacccg cgggagtcgg cggggtcggg cagcccgccg
     7021 ggctggtggt ggcgctcggc ggtacggcgc tgatcgcgga cgagctgctg cggcagcagt
     7081 tcgaggaggc acagcgggcc gcggcgggca gtggctccgc ctcgtccggc agctccgcgt
     7141 ccgacacctc cgggggcggc tcggggagcg acggtggctc ctggtgcggg tcctccgacg
     7201 gcgggtccgg gtgcggcagc ggcggctcgt cgtgcggcgg cggtgcgagc tgtgggggcg
     7261 gctcgtcatg cgggggcggg tcgagctgtg gcggcggagg ctgcgggggc ggctgaaccc
     7321 ggcgggcaac tgcccgcatg ctgagaaact tgtggcggcc gttgcgtcac atcgcataaa
     7381 aactccggca tgtggctgct cttcttcctc gtcgcctgtt cggcggcggt gctgtcctgt
     7441 gcgctgctct gccgtgcggc ggtcgccgcg gcccgcgcca cccccgggcc ggccccgcgg
     7501 ccggctcccg agaccgtccc ggatgcggcc gccgcgcgcc gcggccccgg cctgaccctg
     7561 tacgaaatgg cgtacctgtc gggcggcccg caccggctcg ccgacgtggt gctggtgctg
     7621 atggcccagc agcggcggct gctgctcgcc gacaccggct gggccaccgt cgtcgacccg
     7681 gtcggcaagg acgccgtcga gcgcgccgcc ctcaccgcca tcggccccga agggcagtgc
     7741 cgcataccgg cgatacggga tgccctggtc ggcgacgacg cggtgcggca ggtggccgac
     7801 cggctggtga ccgacgggct ggcggtgccc gccgcggtcc gtccgggcgt cggcagcgcc
     7861 gtccgcctgg tgcagggcgc cgctctgctg gtgctgctgt ccgccgcggc gtcctactgg
     7921 atggcaccgg ccggcgcgga caccgggctg ctgccggcct ggttctcgct gccgctgatc
     7981 ctcaccctcg ggacctgggc cgtggcccgc ttcgagctcc acccgtacag cgactgggcc
     8041 accaccgccg gcgagcggca actgcccggc gtgagccact cccccgccgc ccctgccacg
     8101 cctgcctccc tggtcacttt ggcccttcgc ggccccgccg ccctcaccga tcccgccttg
     8161 cgcgccgcgc tccactcttc cggggcatag cctccgacac cggtgccagg tgctttacaa
     8221 cgacaatcgc cgccccaact atcccttttg tatgcacaca cgaagggacg gccagtgaga
     8281 gcacttgcgt tgtacggcac catcgggtcg ctgctggtga ccgccctcgc cactgccccg
     8341 gcaggcggcg ccacgggggc cgccgccgcg ccacccgcac ccgttccgca ccgcatcgaa
     8401 ttcggccgct gcgccgcggt ggagcatctg ccgtcgaccg tcgagtgcgg cagactcacc
     8461 gttcctctcg actacgcgcg ccccgacggc aagaagatcc acctcaccgt cagccggatc
     8521 cgcgccacgg cgcgcggcga gcggcagggc gccctggtct tcaaccccgg cggccccggc
     8581 gcctccagca tggagttccc gctctacggg gggctcccca agtggcgcaa ggtggcccgt
     8641 gcctatgact tcgtgggcta cgcgccacgg ggtgtgggcc gctcggcgcc gctgtcgtgc
     8701 cagaacccca aggagttcgc caaggcgccc accgacagcc ggatgcaccc cgatgccgcc
     8761 ttcaagcggc agaagcgggc ggaggccagg gagtacgccc gcggctgtgt ccgccgggcc
     8821 ggcgcggacc tgccgtacta caactccgtc aacaacgccc gcgacctgga catgctgcgg
     8881 gccgcgctcg gcgagaagaa gctgacgttc atgggcgcct cgtacgggac gtacttcggt
     8941 gcggtctacg cgacgctctt ccccggccac gtacgacgga tgatcttcga cagtgtggtc
     9001 aatcccgcgc cccggaagat ctggtaccag aacaacctcg accaggacgt cgccttcgag
     9061 cgccgctggg gcgactggct gggctgggtc gcccggcacc atgcgcacta ccacctcggt
     9121 acgaccaaga aggcggtgca gcaggcctac aacaaggccg cgggcaagct gcgccgcaca
     9181 ccgctcaacg ggaagatcgg cgccggccag ctccagtcgg cgttcctgaa gaccggctac
     9241 aacgacgcct tctggccgat gcgcgccgag gcgctcgcgg ggtacctgca cggcaacccc
     9301 aagccgctga tcgcccaggc cctgccccgg gacaccggcg ccgccgccga cgagaacagc
     9361 aacgccgtct acacggccgt ggagtgcaat gacgcgccct ggccgcacga ctggcggacc
     9421 tggaaccggg acaacacgga gatcgcccgg atcgccccgt tcgagacctg ggacaacgcc
     9481 tggatgaatc tgccgtgcgc ctactggccc gaccggccgg cgtcggccgc cgccgagctc
     9541 gacgacgcgg tgcgccgcgc ggaccatccc gccgccgggc gtgacctggc cgatgtcgcc
     9601 gggcatgtgg cccggatccg gatcgatgac gtactccatg ggcggcgggg tccgctcgat
     9661 gtccatacgg agcccggtgc gctgccgccg gtgctgctgc tggcggccga gcgggacgcg
     9721 gcgacgccgt accggggggc gctggagctc cagcgccggc tgcccggctc ctcgctggtc
     9781 accgagaacg gcgcgggcac gcacggcgtc gcgttcaacg acaacgcctg tgtgaccaag
     9841 tacgccgagg cgtatctgct gcacgggaag atcccggcgc acaaggtcta ctgcgcaccg
     9901 cgcgcggaac cgctgccggg ccagcaggcg acccgggccc agcacgccct gcccgggaag
     9961 tagccgaccc cccttgcgcc gcgggccgtc cctgaggggg cggcccgcgg tgtggtgtcc
    10021 ggggcccgcc cggacgcgcg cgtccggtta cgccagaccc gcgaccagct cggcgaccgg
    10081 cttgcgccgc cccgtgtaga acgggatctc ctcgcggacg tgcatccgcg cctcggagcc
    10141 gcgcagatgg cgcatgaggt cgacgatgcg gtagagctca tcggcctcga aggccagcac
    10201 ccactcgtag tcgccgagcg agaaggacgg cacggtgttg gcgcgcacgt ccgggaagcc
    10261 tcgggccatc ttgccgtggt cggccagcat ccggcggcgg tcctcgtcgg ggaggaggta
    10321 ccactcgtag gagcgcacga aggggtagac gctcacatag gcgcgcggct gctcgtcggc
    10381 caggaaggcc gggatgtgcg acttgttgaa ctcggcgggg cggtgcagcg ccatgttcga
    10441 ccacaccggc tccagcgcac ggcccagacg ggtccggcgg aagaggttgt acgcctcctg
    10501 gagggcgtcc gagctctccg agtgccacca gatcatcagg tccgcgtcgg cccgcagacc
    10561 ggacacgtcg taggtgccgc ggaccaccac gtccttggcc gcgagctggg cgaacagctc
    10621 ctcgacctcg tcggcgtagc cgctgcggtc ctccgggagg acatcgcgca gcttgaacac
    10681 ggaccagagg gtgtagcgga tgacctcgtt gaggtccttg gccttcttgc cggcgtgcgg
    10741 caccttctcg ggcgccgcgg gggtctgcgg cgcggaagcg ggagtggaag cgtctgtcat
    10801 gtggtccatt ctcactcggc ggggactgtg tccggcgtca gggtgccgag gagctcgtcc
    10861 gcggcgcggc gcgccgagcc gatgcaggcc gggatgccga cgccgtcgta gaccgcgccg
    10921 cacacccgca gcgcacccgg catccgggcg accgccgcgc ggatcccggc gacccgctcc
    10981 agatggccga cggcgtactg cggcagtccg ccttcccagc gggtgaccgt gctcgccacc
    11041 ggccgggcgg tcaggccgac cgccgcgccg aggtcggcga gcgagaggtc gaccagttcg
    11101 gagtcgtcgc gctggaggtc ggcggtgtcc tggtaccggc cgagggaggt gcgcagcacg
    11161 aagaggtcgg ggtcggcggc gtcgatccag ccccatttgt ggctggcgaa ggtcgaggcc
    11221 ttgatgcggt ggccgtcgac gggcggtacg aggaagccgc tgccgccggg cacccggtcc
    11281 aggtcggcgc ggcggaaggc catggtgacc agcgccatcg aggcgtagtc
//
