LOCUS       NZ_BBNO01000007        10921 bp    DNA     linear   CON 19-AUG-2024
DEFINITION  Streptomyces lydicamycinicus strain TP-A0598, whole genome shotgun
            sequence.
ACCESSION   NZ_BBNO01000007
VERSION     NZ_BBNO01000007.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces lydicamycinicus
  ORGANISM  Streptomyces lydicamycinicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Komaki,H., Hosoyama,A., Igarashi,Y. and Tamura,T.
  TITLE     Streptomyces lydicamycinicus sp. nov. and Its Secondary Metabolite
            Biosynthetic Gene Clusters for Polyketide and Nonribosomal Peptide
            Compounds
  JOURNAL   Microorganisms 8 (3), 370 (2020)
   PUBMED   32155704
  REMARK    DOI:10.3390/microorganisms8030370 Publication Status: Online-Only
REFERENCE   2
  AUTHORS   Komaki,H., Ichikawa,N., Hosoyama,A., Fujita,N. and Igarashi,Y.
  TITLE     Draft genome sequence of marine-derived Streptomyces sp. TP-A0598, a
            producer of anti-MRSA antibiotic lydicamycins
  JOURNAL   Stand Genomic Sci 10, 58 (2015)
   PUBMED   26380643
  REMARK    DOI:10.1186/s40793-015-0046-5 Publication Status: Online-Only
REFERENCE   3  (bases 1 to 900839)
  AUTHORS   Hosoyama,A., Kimura,A., Ohji,S., Ichikawa,N., Yamazoe,A. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2014) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Newbler v. 2.6
            Genome Coverage                   :: 93x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina HiSeq 1000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000829715.2-RS_2024_08_19
            Annotation Date                   :: 08/19/2024 02:08:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,323
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,092
            CDSs (with protein)               :: 7,092
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 4, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 54 of 145
            Pseudo Genes (incomplete)         :: 123 of 145
            Pseudo Genes (internal stop)      :: 9 of 145
            Pseudo Genes (multiple problems)  :: 39 of 145
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:07:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 843510
            Orig. end                         :: 854431
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBNO01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10921
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="9"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10921
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10921
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5921](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5921)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            255..761
                     /locus_tag="TU31_RS26170"
                     /old_locus_tag="TPA0598_07_07570"
     CDS             255..761
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093683.1"
                     /locus_tag="TU31_RS26170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_07570"
                     /product="lamin tail domain-containing protein"
                     /protein_id="WP_042158778.1"
                     /transl_table=11
                     /translation="MSRNASRIAAAVLGSAALAATAVLPAAAAGHGHHAPAPHHHHRSA
                     VSLGAIQYDSPGRDDYSNRSLNAEYVTVKNNGHRPVNLRGWTLSDRSGQTYRFGNVRLR
                     GHSQVRVHTGIGRDNRWDLYQDRRTYVWDDSDTAILRNDHHRIVDIESWGRHSGHHHGH
                     WGHSH"
     gene            complement(1214..2887)
                     /locus_tag="TU31_RS26175"
                     /old_locus_tag="TPA0598_07_07580"
     CDS             complement(1214..2887)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995544.1"
                     /locus_tag="TU31_RS26175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_07580"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_042158779.1"
                     /transl_table=11
                     /translation="MDYFDLGTFTRPVSTRSPEAQLWFDRGLVWSYAFNHEEAVTCFEA
                     AAAADPGCAMAYWGIAYALGPNYNKPWEFFDDADLRRSVERAHTAVERAHALCDGATPT
                     ERALIEALRERYPASRPATDCSLWNEPYAEAMRAVYELAPDDLDIATLYADALMNLTPW
                     QLWDLRTGEPVVGARTLEAKAVLDRALLTATGSDHPGTLHMYIHLMEMSPTPEKALSVA
                     DRLRGLVPDGGHLHHMPSHLDVLCGDYRRVVSGNSDAIKADERYLRRAGAMNFYTLYRS
                     HNYHFKIYGAMFLGQSRTALETAAQLEASIPEELLRVESPPMADWLEGFLAMRVHVLIR
                     FGRWTDILALPMPSDLELYCVTTAMLHYARAVAYSATGATDEAEAERELFRRAAERVPE
                     TRMLFNNTCRDILAIASAMLDGELAYRKGEYDAAFASLERSIELDDNLPYDEPWGWMQP
                     TRHAYGALLLEQGRVEEAEAVYRADLGLDNTLPRPLQHPGNVWALHGFHECLVQLGKTG
                     EAGIVAQQLTVATALADVPVEASCFCRLDTAGAKSGYSSD"
     misc_feature    complement(3308..3317)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     gene            3592..4005
                     /locus_tag="TU31_RS40655"
                     /old_locus_tag="TPA0598_07_07590"
     CDS             3592..4005
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="TU31_RS40655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="TPA0598_07_07590"
                     /product="hypothetical protein"
                     /protein_id="WP_063770827.1"
                     /transl_table=11
                     /translation="MRDWHRPLDLLPGGIETSIDADSTLTDTVDRIMLATGLACLPRAQ
                     ALRAMPVDSPVALGQCERSAVWDGSAGAVGSCLTKLGGANVLDFSRAAVDGSRSRAARS
                     DIRRWIDLGQGAEPRPFRRSGGGSRYAYQRGAP"
     gene            complement(4066..4776)
                     /locus_tag="TU31_RS26185"
                     /old_locus_tag="TPA0598_07_07600"
     CDS             complement(4066..4776)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006601759.1"
                     /locus_tag="TU31_RS26185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_07600"
                     /product="HAD family phosphatase"
                     /protein_id="WP_042158780.1"
                     /sec_met_domain="HAD_2 (E-value: 1.9e-28, bitscore: 95.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTADSILSWTPAAVVFDCDGTLMDTERHWEDARDMVLNSYGCAPS
                     DEFADKARGLHYTECGRLMAEVAGRPELTGDMTESLLKHFRELVAADPTTMPGAPELVH
                     STVQFAPLAVASNCPLEVVESCLGMAGLRDCFSHIVVPDDTTRPKPHPDVYLTAAQLCG
                     VDPADTLAVEDSHCGIQAAAGAGMRVLGVGPRPSDRETALADWWVHSLAEPAVQGWADA
                     RIPAQGTAPGTTTA"
     misc_feature    complement(4588..4590)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(4926..4941)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            complement(5001..5921)
                     /locus_tag="TU31_RS26190"
                     /old_locus_tag="TPA0598_07_07610"
     CDS             complement(5001..5921)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543192.1"
                     /locus_tag="TU31_RS26190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_07610"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_042158781.1"
                     /sec_met_domain="AfsA (E-value: 1.4e-15, bitscore: 52.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTSTVRAVGKSEPLSWSRTVERELVHRTSVAEVLLTDVRPTDTP
                     HVFLAAASWPRAHATFPRDGSQRHSPLILVETLRQLGLYIPLRFYAVPEVFRAVITDLH
                     FHLSPADELPARAGATEITCSARVSAVRRVPDESAVGLRLNVTFAACGVTFGRGGGGVR
                     FLDGARFAALRARSAVHAVPARTPEGGYRPPAAQLTVNHPHDAVLALDQGELLVSPADP
                     LHPFFFDHPTDHVPGMVLLEAARQAVSYRSRGRLSRPLFGRLKASRFTELTPPARVLCT
                     RHGSTCAFRILQGGTETACGTFGYT"
     gene            6021..6641
                     /locus_tag="TU31_RS26195"
                     /old_locus_tag="TPA0598_07_07620"
     CDS             6021..6641
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006601757.1"
                     /locus_tag="TU31_RS26195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_07620"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_078885952.1"
                     /transl_table=11
                     /translation="MYVQIRAETTRRFLLEAAATLFDEKGYAGTSISDISAHSGRTSGA
                     IYFHYASKEKLALAIVEEHFATWPHLISRYRQPGLPALERLVGLSFEVARAFRDDIVVR
                     AGARLWAERKAIDAQLPAPFLGWIEAVTQLLEEAARNGELAADVPPAATAHGIVCSFFG
                     LHTVSDALEGRRHIEERLRDLWTLLLRGLQAEPDPAALLARVR"
     gene            complement(7375..7710)
                     /locus_tag="TU31_RS26200"
                     /old_locus_tag="TPA0598_07_07630"
     CDS             complement(7375..7710)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="TU31_RS26200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="TPA0598_07_07630"
                     /product="hypothetical protein"
                     /protein_id="WP_245698725.1"
                     /transl_table=11
                     /translation="MDGSSGPSKRDEFVISGNLSRGGVPVGTYSQICTLTRTAPADEFD
                     LQSAADLALPLGQLTVQGRLTAIGAGPGNIVLASTGGTGRYRTAHGTVHGDNVSGRETQ
                     LTVHLIR"
     misc_feature    complement(7516..7518)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(7711..7725)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     gene            complement(8079..9668)
                     /locus_tag="TU31_RS26205"
                     /old_locus_tag="TPA0598_07_07640"
     CDS             complement(8079..9668)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1173: WD-40
                     repeat-containing protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092414.1"
                     /locus_tag="TU31_RS26205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_07640"
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="WP_042158782.1"
                     /transl_table=11
                     /translation="MGDTPEGITLTPDGARAYVANRGSNTVSVIDTATNTVIDTIATGA
                     GPTDVAISPDGTRAYVAVFDDGLVSVFDLASNTLVTDIPVGAGATMAAVTPDGTRVYVT
                     RQTTDTVSVIDTATNTVTDTVTVGTGPTGVVITPDGTRAYVASFSGSVSAIDTATNTVI
                     ATILAGDVPILLAVSSDGTRVYVTNAGSSSVSVIDTATNTTTDNIGVSAQPRFPALSPD
                     GAHLYVPNSEPDTVSVIDTATRTVVENISAGDGPTGFAVFPDGTRAYVGNVNAGTVEVM
                     ATTVIPDQGPMAGGTTVTLTGHHLANATAVHFGTAQAVITANTATSVTVTSPAGAGVVP
                     VTVTTAGGTGTLGSFYYMPTPALTGISPDAGPVSGSDQEIVITGRNLAGAIDVYFGTTR
                     AVIQSVSNTQVTVRAAPAPAPGAVAVTVITSGGSADGPFYTYVGQSTITDVSPSAGPTT
                     GGTGVTITGTGLAYTEQVTFNGTPAPFAVISDTTVTAVSPPSATEGTFDVTVTTPGGSS
                     SGSFTYVFPPGV"
     gene            10089..>10376
                     /locus_tag="TU31_RS26210"
                     /old_locus_tag="TPA0598_07_07650"
                     /pseudo=""
     CDS             10089..>10376
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576064.1"
                     /locus_tag="TU31_RS26210"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_07650"
                     /product="acetyl-CoA acetyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTSGTTYGNRKVAVAGVALSDRGRVDRATPYALHAQAARRALADS
                     GLDRAVIDGFASAGLGTLAPVEVAEYLGLRPTWVDSTSVGGTTWEALAAHA"
ORIGIN
        1 ctgtcctggt gatgcaccgg tggaaacccc atggggcgtc tggatgcatg tacggggcgt
       61 cgcatcggag ctggtgagcg cgggggagtg tgcgcctggc ttgctggggt tgtgtatatg
      121 tccgtgtttt gtaacaccaa accggatttc ttccgccaag gcgtgaccgg ccgtcactgt
      181 gtgggggccc ggtcaaaccg gccgggcgtc cggtttccgg cgccctgtcg tggcgccgta
      241 tccagggggt tttcgtgtcc cgtaacgctt cacgtatcgc cgccgctgtc ctcggctctg
      301 cggctctcgc ggccaccgct gtcctcccgg ccgccgcggc cggccacggc caccacgctc
      361 cggccccgca ccaccaccac cgctcggcgg tgagtctcgg ggcgatccag tacgacagcc
      421 ccgggcgcga tgactactcc aaccggagcc tgaacgcgga atatgtgacg gtcaagaaca
      481 atggtcaccg tccggtcaac ctccgcggat ggaccctgtc cgaccgctcg gggcagacct
      541 accgcttcgg caatgtgcgc ctgaggggcc acagccaggt ccgtgtccac accggcatcg
      601 gacgcgacaa ccgctgggac ctctaccagg accgccgcac ctacgtgtgg gacgacagcg
      661 acaccgccat cctgcgcaac gaccaccacc gcatcgtcga catcgaaagc tggggccgcc
      721 acagcggaca ccaccacggt cactggggcc acagccactg aaaatgagcc gctggtgaga
      781 cggtgagact tggtcactcc caccagcccc tggtgcgccg cagtcctgcg tctgcggcgc
      841 accgccatgt ccggctccgg gccgcggagt atgtccccgc gggccgggcg ggggcaggcc
      901 cacgtggtgt ctggggccgt ttgagcgggt gccgaggtgg ccgaggtcga gtcgacggcc
      961 agcccaggtc agggcatccg caggaggcat ggggagtgcc ggcaaaccgt agacagacca
     1021 cggccccacg ccggtctcca ggacggcatc accagttcgg gaaaaccccc gtcgcccagg
     1081 ctacgagcgc gcggagcaac agcggccgga gagccgcgcc gcgatggtcg cggttcgcgg
     1141 gggagtgagg cgaagtgcag caggcgggta gtgaggaagc cgttacacgg catcagttcg
     1201 cacggtcacc agctcagtcg gagctgtagc cgctcttcgc gcccgcggtg tcgaggcggc
     1261 agaagcacga cgcctcgaca gggacgtcgg cgagtgcggt ggccacagtg agttgctggg
     1321 cgacgatgcc ggcttccccc gtcttgccga gctgcacgag gcattcatgg aagccgtgga
     1381 gggcccagac gtttcctgga tgctgcaagg gcctgggcag ggtgttgtcg agcccgaggt
     1441 cggcccggta gacggcctcg gcctcttcga cccggccctg ttccagcaga agcgccccgt
     1501 aggcgtgacg ggtcggctgc atccagcccc acggttcgtc gtagggcagg ttgtcgtcga
     1561 gctcgatgga gcgctccagg gaggcgaacg ccgcgtcgta ctcgcccttg cggtaggcga
     1621 gttcgccgtc aagcatggcg gaggcgatcg ccaggatgtc ccggcacgtg ttgttgaaca
     1681 gcatccgcgt ctcgggaacc cgttcggccg cgcggcggaa cagctctcgt tcggcctcgg
     1741 cctcgtcggt tgcccccgtg gcggagtagg ccactgcgcg cgcgtagtgc agcattgccg
     1801 tggtgacgca gtacagctcc aggtcggagg gcatcggcag cgcgaggatg tctgtccacc
     1861 ggccgaagcg gatcaacacg tgcacccgca tggcgaggaa gccctccagc cagtcggcca
     1921 tgggcgggct ctccacgcgc agcagctcct cggggatgga cgcctccagt tgcgcggccg
     1981 tctccaatgc cgtcctggac tggccgagga acatcgcgcc gtagatcttg aagtggtagt
     2041 tgtgcgagcg gtacagggtg tagaagttca tcgcgccggc tcgccgcagg tacctctcgt
     2101 cggccttgat cgcgtcgctg ttgcccgaca cgacccgtcg gtagtccccg cacagcacgt
     2161 cgagatgtga gggcatgtgg tgcagatggc ccccgtccgg gaccagtcct cgcagtcggt
     2221 cggcgaccga aagagccttc tccggtgtcg gcgacatctc catcaagtgg atgtacatgt
     2281 ggagcgtgcc cggatgatcg ctgccggtcg cggtcagcaa cgcgcggtct aggacggcct
     2341 tcgcctccag cgtgcgcgcg ccgacaacgg gctcgcccgt gcgcaggtcc cacagctgcc
     2401 acggggtgag gttcatcaac gcgtccgcgt agagggtggc gatgtccagg tcgtcgggtg
     2461 cgagttcgta tacggcgcgc atggcctcgg catagggctc gttccagagg gaacagtccg
     2521 tcgcggggcg cgaggccggg tagcgctcgc gcagcgcctc gatgagcgcg cgctcggtcg
     2581 gggtggcgcc gtcgcacagt gcgtgggccc gttcgacggc ggtatgcgcc cgttccaccg
     2641 agcgccgcag atcggcatcg tcgaagaact cccagggctt gttgtagttg gggcccaggg
     2701 cgtacgcaat gccccagtac gccatggcgc agccgggatc cgctgctgcc gccgcttcga
     2761 agcaggtgac cgcctcttcg tggttgaacg cgtagctcca tacgaggcca cggtcgaacc
     2821 agagctgtgc ttccggtgaa cgtgtcgata cgggccgggt gaaggtcccg aggtcgaagt
     2881 agtccatgag gatctcctgc ggagggatgt gactcgacga cagtcggcgc tcagtgcgaa
     2941 gagggcgccg atcgaggctt tgcggttggc ctccgcattc gacgaacaga gcagcccgca
     3001 gtgccttctt cctgctgaca gcgggcgcag acagctatcc gcagactctt tctgtgctct
     3061 tcggccggag cgcggcgggg catctccagc gcacgccgcg tgatcgggtc ggctccggcc
     3121 acaagccccc ggtgtcggcg caccgggtga tcacgtccga cttcctgacg cagggacgtt
     3181 cctggaagcg accgggaggc cggcgaagga ggcgaggcgc atgacgtggc tgtcgagcac
     3241 actgttgtcg atcttgtaca cgcgagacac ggcatctcct tgccctccgt tgagccgcct
     3301 cggtcagcgc cgccttcgtt tcgttggact gcttcagcgt ggtgaccatg tccaccgggc
     3361 ggaccaaggt gccctggatg gacgaacgtc gctgcaccgc ctcgacgagg tccgcctgct
     3421 cctggcggag cgctcccggg gagtcatttt cgactgtaac ccggctgtgg ccggcgcccg
     3481 gatactgctc gggtgccacg ccgaggaaca ccgccagaat gcaaccggcc accagatcca
     3541 ccccgcccgc cacgccccga gccgatcgct gacgaggtag ccgagccgca gttgcgcgac
     3601 tggcaccggc cgctcgatct cctgccaggc ggcatcgaga caagcatcga cgccgacagc
     3661 accctcaccg acacggtcga ccgcatcatg ctcgccaccg gtctggcctg ccttccgcgc
     3721 gctcaagcgc tgagggcgat gcccgtcgac tcgcctgttg ccctcgggca gtgtgagcga
     3781 tccgcggtgt gggatggctc ggccggagcc gtgggaagtt gcctgaccaa gcttggtggc
     3841 gcgaacgtcc tggacttctc ccgggcggcc gtcgacggct cccgctcacg ggcagcgcgc
     3901 agcgacatcc gccgatggat cgacctcggc cagggcgccg agccgcgccc cttccgaaga
     3961 tccggcgggg gctcccggta tgcctaccaa cggggcgccc cctgaatgcc acccggcccg
     4021 cgggcccgcg gcccgtcacg gagtctgccg cgggccgggc cgccgtcatg cggtcgtggt
     4081 cccgggcgca gtgccctggg cagggatgcg ggcgtcggcc catccctgca ccgccggctc
     4141 tgccagcgag tggacccacc agtcggccaa cgccgtctcc ctgtcgctgg ggcggggacc
     4201 gactccgagt acccgcatgc ccgcacctgc ggcagcttgg atgccgcagt gggagtcctc
     4261 gacggccagc gtgtcagcgg ggtccacccc acagagttgg gcggcggtga ggtagacgtc
     4321 cggatgaggt ttgggtctcg tggtgtcgtc gggcaccacg atgtggctga agcagtcccg
     4381 gagtccagcc attcccagac aggactcgac gacttccagc gggcagttac tggccacggc
     4441 cagcggggcg aactgcacgg tcgaatgcac cagttccggc gccccgggca tggtggtggg
     4501 gtctgcggcg acgagttcac ggaagtgctt gagcaatgat tccgtcatgt ccccggtcag
     4561 ttccggtcgc ccggccacct cggccattaa gcgcccgcat tcggtgtagt gcagccctcg
     4621 cgccttgtcc gcgaattcgt ccgagggtgc gcagccgtag ctgttcagca ccatgtcacg
     4681 ggcgtcctcc cagtggcgtt cggtgtccat cagcgtgccg tcacagtcga aaacgacggc
     4741 cgcgggggtc caggaaagga tgctgtctgc ggtcattcgg cattccgttc tggtggggcc
     4801 cgaaggcgcc gatgcgaccg gtcgcgagag aggcagggca gcgtcggttt cacggacgcc
     4861 cggcaccggg ccgagggatt ccggcagggc agctctcacg gataggagtt catccgctcc
     4921 agccggaaaa taacgatcgt tacgttacgg acggcaacgc agaaaggtca accaattcat
     4981 aaatgtcaat attatgtgct ctacgtataa ccgaacgtgc cgcaagccgt ctccgtgccg
     5041 ccctggagga tgcggaatgc acacgtcgag ccatgccggg tgcacagcac acgtgcgggt
     5101 ggggtcagtt cggtgaaccg ggaggctttc agccggccga aaagcggccg gctcagtctg
     5161 ccccgactgc ggtacgagac ggcctggcgg gcggcttcca acagcaccat gccaggcacg
     5221 tgatcggtgg ggtggtcgaa gaagaacggg tgcagcgggt cggccggcga gaccaacagc
     5281 tcgccctggt cgagagcgag gaccgcatca tgcgggtgat tgacggtcag ctgtgcggcc
     5341 ggcgggcggt acccgccctc cggagtgcga gccgggacgg catggaccgc gctcctggcg
     5401 cgcagcgcgg cgaagcgcgc gccgtccagg aatcgcacgc ccccgccgcc tcggccgaaa
     5461 gtcaccccgc acgcggcgaa ggtgacattc agtcgcagtc cgacggccga ctcgtccggc
     5521 acccgccgta cggcgctgac ccgtgccgag caggtgattt cggtcgctcc ggcgcgggcc
     5581 ggcaactcgt cggcggggct gaggtggaag tgcaggtcgg tgatgaccgc acggaagacc
     5641 tccggaaccg cgtagaaccg caagggaatg tagagaccga gctgacgcag tgtttccacc
     5701 agaatcagcg gactgtgccg ctgcgaaccg tcgcggggga acgtcgcatg cgcgcgtggc
     5761 catgacgccg ccgccaggaa gacgtgcggg gtatcggtcg gacgtacatc cgtaaggagg
     5821 acctctgcca ccgaggtccg gtgcaccagc tcccgctcga cggtacggga ccagctgagc
     5881 ggctcactct ttccgacggc cctgaccgtt gaggtgctca tacgaccagg gaataccgaa
     5941 attcgaacat gcgcaccctt gaattccggg gcgcggggag cggtaggttg ccttccggcg
     6001 gaacgagaac aacgtttgag gtgtacgtgc agatccgggc ggaaacgacg cgcaggttcc
     6061 ttctcgaggc ggcggcaacc ttgttcgacg agaaagggta cgccggtacc agcatcagcg
     6121 acatcagtgc gcattccggt cgcaccagcg gggccatcta tttccactac gccagcaagg
     6181 agaagctcgc cctcgccatc gtcgaggaac atttcgccac gtggccgcat ttgatcagcc
     6241 gctaccggca gcccggcctg ccggccttgg agagactcgt cgggctgagc ttcgaggtgg
     6301 cacgcgcctt ccgagacgac atcgtcgtcc gggccggagc ccgcctttgg gccgaacgca
     6361 aagccatcga cgcccagctc cccgcaccgt tcctcggctg gatcgaagcc gtgacacagc
     6421 tcctcgaaga agccgcccgt aacggtgaac tcgctgccga tgtgccacct gccgccaccg
     6481 cccacggcat cgtctgctcc ttcttcggtc tgcacaccgt ctccgacgcc ctggagggac
     6541 gccggcacat cgaggagcgc ctgcgcgacc tgtggactct gttgctccgc ggcctccagg
     6601 ccgagcctga ccccgcggct ctgctcgctc gcgtgcgctg agcggcgcgc gacacccacc
     6661 gatcttgcag acgttgagga aggccgcagt ccgcagctgc gcctgttcca gtggacgggc
     6721 cgcggggatc gtcggggggc gtggccgtgc accagggtgt gcagtgcggg gtgcccggtg
     6781 agagcgcccg cgctgcggcg gtcctctgtg cgaactcacg gggtcgcccc gcgtccgcac
     6841 cggcctcggc ctggccgctg gcgtgcgatg tcgaggtggt cgacggggca gtacggcacg
     6901 gatgctttgc aggtgagggt gagcagcatt tgtcgacggt ttcgggcagc actgagcggt
     6961 cacagagctt ggaaaagtcg acgagaacta ggaacgctgt cagagcgtgt ggacccttct
     7021 ccggtgtcgt gatggtggct gaacttcggg ctccgtcaag gtcgttcgtc ttcggcttcg
     7081 cctgcgggcg gctccaccga gacgcggtgc gaagtccagc gatgcggttg cggcctgccg
     7141 gcagggcggt tcttcttctt gcgcggacat ggcctcccgc cggacttggt gtacggcacg
     7201 tatcacgagg cgcgcgcctt cttccggcag ccgctcaacg agcaggtgcg cttcaactcc
     7261 gggggcaaag agtggcgcac gccgggccgg gccgcgtcgc cgtggcccgg cccctcgaac
     7321 tcacgtgggc ggccccggag tcacctgggg ccacctcggg cgccggccgc ccggtcagcg
     7381 gatcagatgc acggtgagct gcgtttccct gccactgacg ttgtcgccgt gaacggtgcc
     7441 gtgcgcggtg cggtagcgtc cggtacctcc ggtgctcgcg aggacgatgt tgccggggcc
     7501 ggcacctata gcggttaacc ggccctggac ggtgagctgt cccaggggaa gggcgaggtc
     7561 ggccgcgctc tggaggtcga actcatcggc gggggcggtg cgggtcagcg tgcagatctg
     7621 gctgtaggtg ccgaccggga cgccaccgcg cgagaggttc ccgctgatga cgaattcatc
     7681 ccgcttgctg ggcccggacg acccgtccac atcgacaaaa gtgacctgcg tctgctgcgc
     7741 ggtcagctgg aatacttctt ctctcggata gtggtcgacg ccggtaccgg tcgcggccga
     7801 tgccgaattc ggtcagaaaa gaagggccgc gaatccggtg gccgcgctca gggcgatgcg
     7861 cttgaagctg aagtgacgca cgatttctcc tgtgggacgg gaagggaaat gcacttggcc
     7921 ggctgccgga aggtgttttc ctgcccgggc tggcatcgcg aggtcggcac ggctccggcg
     7981 ctcagggccc gttgccgggc aaccgagcgc ccggctggcc gggatctcgc tgctccggcc
     8041 cggatgcggc gcccgcccgg acggcgggcg cgggtgggtc agacgccggg tgggaagacg
     8101 taggtgaacg atcccgaaga gctgccgccg ggggtggtga cggtgacgtc gaaggtgccc
     8161 tcggtggcgc tgggcgggga cacggcggtc acggtggtgt cggagatgac cgcgaagggc
     8221 gccggcgtgc cgttgaaggt gacctgttcg gtgtaggcca ggccggtgcc ggtgatcgtc
     8281 accccggtgc cgccggtggt cggtcccgcg ctggggctga cgtccgtgat ggtggactgg
     8341 ccgacgtagg tgtagaacgg tccgtcagcg ctcccgccgc tggtgatcac ggtgacggcg
     8401 acggcgcccg gggccggggc aggggcggct ctgacggtga cctgggtgtt ggagacactc
     8461 tggatgacgg ctcgggtggt gccgaagtac acgtcgatcg ctccggcgag gttgcggccg
     8521 gtgatgacga tctcctggtc gctgcccgag acggggccgg cgtcggggct gatgccggtg
     8581 agggcgggcg tcggcatgta gtagaaggac ccgagggtgc cggtgccgcc cgcggtggtg
     8641 accgtcacgg ggacgacacc ggcgccggcg ggggaggtga cggtcaccga tgtcgccgta
     8701 ttggcggtga tcacggcctg ggcggtgccg aagtgcacgg cggtggcgtt ggccaggtgg
     8761 tgaccggtca gcgtcacggt ggttccgcct gccatgggcc cctggtcggg gatcacggtc
     8821 gtcgccatga cttcgacggt gccggcattg acgttgccca cataggcgcg tgtcccatcg
     8881 gggaagacgg cgaagccggt ggggccgtct cccgcgctga tgttctcgac gacggtgcgg
     8941 gtggcggtgt cgatcacact caccgtgtcc ggttcggaat tcggcacgta cagatgggcg
     9001 ccgtcggggc tcagcgccgg gaagcgcggc tgggcgctga cgccgatgtt gtcggtggtg
     9061 gtgttcgtgg cggtgtcgat cacactcacc gaggagctgc cggcgttcgt gacgtagacg
     9121 cgggtcccgt cggagctgac cgccagcagg atggggacgt cgccggcgag gatcgtggcg
     9181 atcacggtgt tcgtggcggt gtcgatcgcg ctgacggaac ccgagaagct cgccacgtag
     9241 gcccgggtcc cgtccggggt gatgaccacg ccggtcggcc cggtcccgac ggtcacggtg
     9301 tcggtgaccg tgttggtggc agtgtcgatc acactcaccg tgtcggtggt ctgccgcgtc
     9361 acatagacgc gcgtcccgtc cggggtgacc gccgccatcg tcgccccggc ccctacgggg
     9421 atgtccgtga cgagggtgtt ggaggcgagg tcgaacacgc tgaccagacc gtcgtcgaag
     9481 acggccacat aggcgcgggt gccgtcggga ctgatcgcca cgtcggtggg accggccccc
     9541 gtggcgatgg tgtcgatcac ggtgttcgtg gcggtgtcga tcacgctcac cgtgttcgag
     9601 ccccggttgg ccacataggc gcgggccccg tccggggtga gggtaatccc ctccggggtg
     9661 tcccccacgg gaataacagc gcctgcgaca aacgcggcat tcaatgcggc gtagggtgcc
     9721 cgggcatgct gcggagatgg tgacgctaag gcgaacatga cactccccaa agaaggcggg
     9781 acggtctttg tcccgcgtat ggaaatgtct gctccgccgc atgggcgaac agcgagcagt
     9841 caaccggccg cgaatccggc cggcgaaata cacacgcaag cggagaatac cctcccgggg
     9901 aaagtattta aagaccgcac gatatctcgg ataatcgctt taaggtgcgg ggtgggcgga
     9961 cctggccgcg acccaccggg cggacggggc tgccgcctgg cgtgtcgcgc cctgcccgcc
    10021 cggccacgtc tgatgtgccg tcagattcat gacctcaggg accacttcat gacctcaggg
    10081 accacttcat gacctcaggg accacttacg ggaatcgcaa ggtcgccgtc gccggagtcg
    10141 cgctgtccga ccgcggacgc gtggaccggg ccaccccgta cgccctgcac gcccaggccg
    10201 cccgccgggc actcgccgac agcgggctgg accgggcggt gatcgacggc ttcgcctcgg
    10261 ccggtctggg caccctcgcg cccgtcgagg tcgccgagta cctggggctg cgccccacct
    10321 gggtcgactc gacgtcggtg ggcggcacca cctgggaggc gttggccgcg cacgccggta
    10381 cgccaactcc gaggtgaggc cggccggacc gccgggtccg caaggccgac ggcgcgctgc
    10441 cccggcaaac accgttgcag cactagcact cctgcggtcg gcggacctcg cccgtgccca
    10501 gtgtgatcac ggcccgcggc cgcatgggcc cctcgccgcg tcgggagagc aggaccacct
    10561 cttcgaccac cgccgacagc gcgccgagcc ggacggcgca ctcggtggcc aggtgcttcg
    10621 ggtcctgggt gcggccgagg gacaaatgcg gggtgaagcg gccggcgcgc ccgtggcaaa
    10681 gcgggaaccg cagtcccagt tcccggtgga gatgggccca cggcgccggg tccgccgcag
    10741 ccggatcgag ccacaacgtc gcgtagtgcc ggtgccggaa gtaccgcact ccggccaggc
    10801 gcgccgtgaa cgccgtgctc tgcgccgcaa cggcggagag cagtggcacg gcccgcgcaa
    10861 acgcttcctc cggcacgaaa ccgaacagca gattcacatg cggcggccac cgccggacct
    10921 g
//
