LOCUS       NZ_BBNO01000007        12941 bp    DNA     linear   CON 19-AUG-2024
DEFINITION  Streptomyces lydicamycinicus strain TP-A0598, whole genome shotgun
            sequence.
ACCESSION   NZ_BBNO01000007
VERSION     NZ_BBNO01000007.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces lydicamycinicus
  ORGANISM  Streptomyces lydicamycinicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Komaki,H., Hosoyama,A., Igarashi,Y. and Tamura,T.
  TITLE     Streptomyces lydicamycinicus sp. nov. and Its Secondary Metabolite
            Biosynthetic Gene Clusters for Polyketide and Nonribosomal Peptide
            Compounds
  JOURNAL   Microorganisms 8 (3), 370 (2020)
   PUBMED   32155704
  REMARK    DOI:10.3390/microorganisms8030370 Publication Status: Online-Only
REFERENCE   2
  AUTHORS   Komaki,H., Ichikawa,N., Hosoyama,A., Fujita,N. and Igarashi,Y.
  TITLE     Draft genome sequence of marine-derived Streptomyces sp. TP-A0598, a
            producer of anti-MRSA antibiotic lydicamycins
  JOURNAL   Stand Genomic Sci 10, 58 (2015)
   PUBMED   26380643
  REMARK    DOI:10.1186/s40793-015-0046-5 Publication Status: Online-Only
REFERENCE   3  (bases 1 to 900839)
  AUTHORS   Hosoyama,A., Kimura,A., Ohji,S., Ichikawa,N., Yamazoe,A. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2014) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Newbler v. 2.6
            Genome Coverage                   :: 93x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina HiSeq 1000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000829715.2-RS_2024_08_19
            Annotation Date                   :: 08/19/2024 02:08:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,323
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,092
            CDSs (with protein)               :: 7,092
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 4, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 54 of 145
            Pseudo Genes (incomplete)         :: 123 of 145
            Pseudo Genes (internal stop)      :: 9 of 145
            Pseudo Genes (multiple problems)  :: 39 of 145
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:07:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 725977
            Orig. end                         :: 738918
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBNO01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12941
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="7"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12941
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12941
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7941](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7941
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    complement(467..487)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.36"
                     /tool="antismash"
     misc_feature    complement(4829..4844)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.78"
                     /tool="antismash"
     misc_feature    4979..4989
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     misc_feature    4982..4997
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.3"
                     /tool="antismash"
     gene            5001..6155
                     /locus_tag="TU31_RS25695"
                     /old_locus_tag="TPA0598_07_06480"
     CDS             5001..6155
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016642316.1"
                     /locus_tag="TU31_RS25695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_06480"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_042158625.1"
                     /sec_met_domain="DAO (E-value: 8.2e-70, bitscore: 231.7,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTRRSSDVIVVGAGVVGAACAYYAARAGLSVTVIDRGPVAGGTT
                     GAGEGNLLVSDKEPGPELDLALLSTGLWQELAELLPPQIEFEPKGGLVVAADETGMRAL
                     RDFAAGQGKAGVRAEEVPADRLRELEPHLTSGLAGGFHYPQDAQVMPALAAAHLLRAGG
                     ERVRLRLGEEVTGLLTTADGAVRGVRTAAGEVSAPYVVNAAGTWGGEFARLAGVELPVL
                     PRRGFVLVTEPLPRVVRRKVYAADYVADVASGSAALQTSAVVEGTAAGPVLIGASRERV
                     GFDRTVSTEVLRRLAAQATALFPALAGVRAMRTYVGFRPYLPDHLPAIGPDPRVPGLLH
                     ACGHEGAGIGLAPATGLLIARSLSGGEPPLDPGPFSPARFEAAA"
     gene            6183..6497
                     /locus_tag="TU31_RS25700"
                     /old_locus_tag="TPA0598_07_06490"
     CDS             6183..6497
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091458.1"
                     /locus_tag="TU31_RS25700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_06490"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_042158626.1"
                     /sec_met_domain="Fer2_4 (E-value: 3.6e-25, bitscore: 83.5,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARTPAELAGANPGPPFEITMDGRPVPALPGQSVAAALWSAGILA
                     WRTTRTTGRPRGAFCGIGQCYDCLATINGEPNRRACLVPASPGDVITTQEGHGHARIGV
                     "
     gene            6478..7941
                     /locus_tag="TU31_RS25705"
                     /old_locus_tag="TPA0598_07_06500"
     CDS             6478..7941
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091457.1"
                     /locus_tag="TU31_RS25705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_06500"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_042158628.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 4.4e-24, bitscore:
                     80.8, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 3.4e-11, bitscore:
                     39.0, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPASASDPYDLAVIGAGAAGLAGAVTAAELGLTVALLDAAPQTGG
                     QFYRHPAPAVGAARPEALHHDWAAFAELRRRLERSDVRRLSGHHVWAVTKEDGDLWAVH
                     AVTGPDGTGEQSVRVRARTVLFATGAYERQLPFPGWTVPGVVGAGGAQAMLKSGLVLPG
                     KRIVVAGSGPLLLAVAASLAAAGAQVPAVIEASGYLGYARHPRALAANPHKLVEAAVHG
                     AALLRHRVRLRTRSAVTEVHGTDRVAAVTVTRLDRDWRPVRGTGRRIACDALAVGHGLV
                     PQIELATALGCATRRTADGTSGLALTGLQETSVRGLWAAGETGGVGGAPLARVEGELAG
                     LAAAARLQGRPADEGRVRALRRRQDRMRAFAEAMAVSHAPAAGWTGWLTDDTEVCRCEE
                     VTAGTIREAVTGLGARDARTVKLLTRAGMGWCQGRMCGTAAACLAAGDGAAEPLAERRP
                     FAVPVPLSALASAGDPAGSRPDAAPPVDD"
     misc_feature    7979..7989
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     gene            8013..8921
                     /locus_tag="TU31_RS25710"
                     /old_locus_tag="TPA0598_07_06510"
     CDS             8013..8921
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091456.1"
                     /locus_tag="TU31_RS25710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_06510"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_042158630.1"
                     /transl_table=11
                     /translation="MTALTWNSTRPWRGIMVATALPLRDDLSVDHDAYAQHVRWLIEQG
                     CDGVVPNGSLGEYQTLTDDERARVVRTAVEAAGDGARVMPGVAAYGSAESRRWAEQAGE
                     AGCGSVLLLPPNAYRADEPAVRAHYAEAAKAGVPVVAYNNPLDTKVDLTPGLLARLHQD
                     GSIVAVKEFSGDVRRAYEIAELAPELDLLIGADDVLLELAVAGAVGWVAGYPNAFPATC
                     AALYHAAVAGDLDTALPLYKSLHPLLRWDSKTEFVQCIKLSMDLVGRHGGPARPPRSPL
                     TGEIEAEVRAATEKAVAAGHH"
     gene            8953..9957
                     /locus_tag="TU31_RS25715"
                     /old_locus_tag="TPA0598_07_06520"
     CDS             8953..9957
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="TU31_RS25715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_06520"
                     /product="proline racemase family protein"
                     /protein_id="WP_042158952.1"
                     /transl_table=11
                     /translation="MRTRHVFHAVDSHTEGMPTRVITGGVGVLPGATMAERRLHFIEHL
                     DHLRTLLMYEPRGHSAMSGAILQPPTRADADYGVLYIEVSGLLPMCGHGTIGVATVLVE
                     SGMVPVTEPVTPVRLDTPAGLVAVDVHVADGVAKSVTLTNVPAFCAGLDRTVEVPGFGR
                     VTYDLAFGGNFYAFVELDALGLPFDRARKDDLLATGLAVMTAINASPDRPVHPEQPEIA
                     GVKHVYLAAPGSDAQHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLNRDFVNE
                     SFIGTRFTGRLVAETEVGGRPAVVPTVTGRAWITGTAQYFLDPDDPFPGGFLL"
     gene            9954..11336
                     /locus_tag="TU31_RS25720"
                     /old_locus_tag="TPA0598_07_06530"
     CDS             9954..11336
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091454.1"
                     /locus_tag="TU31_RS25720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_06530"
                     /product="aldehyde dehydrogenase"
                     /protein_id="WP_042158631.1"
                     /sec_met_domain="Aldedh (E-value: 1.9e-124, bitscore:
                     411.3, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAVASVTSRNPADPSDVLVSVPAPGAFAAADAVERARAAQPAW
                     LAGGAAARSAALGAVATALEAAADELAALAVREVGKPLTEARAEIARSVAIWRYYAQAP
                     YEPLGAVHETAVGPGLLLTRRRPYGVAGLLTPWNFPFAIPSWKAAPALAAGNAVVLKPA
                     PEATACARRLASLVEQALPTGVCTVLPGGATEGNALVSVADVISFTGSTTVGRAVVQAA
                     TARGVPVQAEMGGLNAALVLPDADIEQAARHIATAIAGYAGQKCTATRRVIAVGPALGP
                     LREALAEALRAVPVGDPADPATVCGPLINEQARDRVTRARDGLSVLAGGTAPGLPGWYA
                     APTLVEQVPPGHQLLREEVFGPVAALLPADDLAHAVRISNSVPYGLVTSVHSAGLDPVL
                     HGLDQLDTGMIRVNAPSTGVDFHLPFGGAKESGHGPREQGRAALEFYTSSRTYTLSTVG
                     AM"
     gene            11384..12070
                     /locus_tag="TU31_RS25725"
                     /old_locus_tag="TPA0598_07_06540"
     CDS             11384..12070
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543023.1"
                     /locus_tag="TU31_RS25725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_06540"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_042158634.1"
                     /transl_table=11
                     /translation="MGHLKHRDLNATRERLRDQVAHALRAALISGELRPGEVYSAPVLA
                     EDFGISATPVREAMLDLAREGLVEPVRNKGFRVTEVNERDLDQYTEIRALIEIPMVGRI
                     TRSAARAELEALRPVAEEIVRAARDHDLIGYLEADRQFHLELLGLSGNDRLVETVGDLR
                     KRSRLYGLTALDERGELIPSAEEHLELLDLMLAGEAKAAEKCMAKHLGHVRSLWARSGT
                     AAKSAT"
     misc_feature    12137..12147
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 19.65"
                     /tool="antismash"
     misc_feature    12583..12597
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
ORIGIN
        1 atgcacacct acaccagcag cgtccacagc ccgtacacga tcactgccac ctacaacagc
       61 agcgacgact tcaccggctc ggtggccacg gccaaccagg ccgtcctgcc ggcggcgacg
      121 accaccacgg tgacctccgt accggacccg tccgtcgccg gtcagccggt caccttcacg
      181 gcgatcgtcg ctccggcgga gcacggcgcg ggtgcgccga ccggaacagt gatcttcgac
      241 ttcggggacg gcacggaccc gtccgcacag ccggtcgtcg ccggagtggc gaccgccgtg
      301 cacgcctacc ccagcccgtc cggcagcccg tacaccgtca ccgccaccta cggcgggggc
      361 accgatttca ccgcttccag cggcaccacc actcagtccg tcggccaggc cgcgtccacg
      421 acggtggtca cctcgctgcc cgaaccggcg gtgaccggcc agccggtgac cgtcaccgcg
      481 accctctcgg ccgccgcgcc cggggacggg acaccgaccg gcacggtcac cttcgacttc
      541 ggtgacggta cgtcacccag cacggtgccg gtcacggccg gggtcgccac gacgacacac
      601 acctgggccg gcacctccgg cagcccgtac accgtcacgg ctgtctacag cggggacatc
      661 aacttcaccc cgtccaccgg taccggcacg cagacgatcg ccccagccgc gaccacgact
      721 accgtcagcg cactgccgac gccctcggtg acggggcagc cggtggacct caccgcgacg
      781 gtctccgccg tcgcaccggg tgccggcacc cccaccggca ccgtcacctt cgacttcggc
      841 gacggcaccc cggccctcac cgcacccctg acggacggca ccgccaccct cacccatgcc
      901 tgggacggta cgtccggcag cccctatacc gtcacggtca cctacagcgg ggacgccgac
      961 ttcagcccct ccaccggtac cggcacacag accgtcggcc cggccgcgac caccaccacc
     1021 gtcagcggac tgccggagcc ctcggtcacc ggccagccgg tgacgttcct cgcgcgggtg
     1081 agttccgacg tcctgggggg cgccccgccg acgggtacgg tcaccttcga cttcggcgac
     1141 ggcaccgctc ccgttaccgt cgccgcggcg ggtggcgtgg cgagcgccgt gcacacctac
     1201 acgagcgcca cgggcagccc gtacacggtc cacgccacct actacggcga cgaccggttc
     1261 accgcctccg tgggcaccga aacgcacacc gtgcagccgt cgttgtcggc gacggtggtc
     1321 agctcgtcgc cggatccctc ggtgacgggc gggccggtgg acatcaccgc gacggtctcc
     1381 gcggccgcgc cgggggccgg gacgcccacc ggcacggtca ccttcgactt cggtgacggc
     1441 accccgtccg tcaccgcacc cgtgacggac ggcaccgcca ccgtctccca cgcctgggcg
     1501 agcacctccg gcagccccta cgccctcacg gccgactaca gtggggacgc cgatttcagc
     1561 tcctccacgg ggaccggaac gcagaccgtc gcccaggccg cgaccacgac catcgtgacc
     1621 tcctcgcccg atccctcggt gacgggcggg ccggtggacc tcaccgcgac ggtctccgcg
     1681 gtcgcgccgg gggccgggac gcccaccggc accgtcacct tcgacttcgg cgacggcacc
     1741 ccctccgtca ccgccacggt caccgccggc gtggccaccg tgcggcacac ctggaccggc
     1801 acctccggca gcccgtacac cgtcacggcc gactacggcg gggacctcga cttcggcccc
     1861 tccaccggca ccgacagcca gaccgtcggc caggcctccg ccgccctgtc cctgttctcc
     1921 tccccggatc cgtccgtggc cggacaggag gtcaccttca ccgccctggt ctccgtggtt
     1981 cccccgggcg cgggcacccc caccgggacc gtcaccttcg atttcggcga cggcaccgcg
     2041 cccgtcaccg caccggtcgt ctccgatgcg gcgaccgtga cgcacgccta cgcgaattcg
     2101 accggcagcc cctacacggt caccgccacc taccacggtg acgacgactt cgccgccgtc
     2161 accaccaccg acctccacag ggtggcggtc agcgccgcca ccacgctgac gaccgtgagc
     2221 tcctccccgg acccctcggt gacgggccgg ccggtgacct tgacggcgac cgtggctccg
     2281 accccgcccg gcgccggggt gccgaccgga acggtcacct tcaccttcgg cgacggaacg
     2341 ccggccgtca cggcctcgct ctccggcggg gtcgccggcg tcacccacgc ctacaccacc
     2401 gcgacgggca gcccgtacac cgtgaccgcg cgctacagcg gggacgccaa cttcagctcc
     2461 tcctcgggca cggacagcca gacggtcgac ccggcgtcga cactgaccac ggtggtcacc
     2521 gcgccggacc cgtccgtggt cggccagccg gtgaccctca ccgcgaccgt cgcaccggtc
     2581 gcaccgggtg ccggggtgcc gaccggcgcg gtcaccttcg acttcggtga cggcactgcg
     2641 cccaccgtga cctcgctctc cgacggcgtc gcccggatca accacaccta ctcggccgcg
     2701 ggcggcccct acacggtgac cgcgctctat gaggggaacg acagcttctc ctcgtcgagc
     2761 ggcagtgaca cccagacggt gggcaggtcc gcgacgacga cgtcggtggt gtcctctcct
     2821 gatcccacgg tggtggggca gttgaccacg gtgacggctt cggtggcgtc ggtcgcgccg
     2881 gggtcggggg cgccgtcggg tacggtcacg ttccatttcg gtgatggcag tccgacgttg
     2941 acggcgccgg tcagtaatgg tctggcggtg gtgacgcacg cttatgggag tacggcgggc
     3001 agtccgttcg cgatctccgc gatgtatgac ggggatgcga acttctcctc gtcgagcggg
     3061 agtgataccc agaccgtcgg taaggcggcc accaccacgt cggtggtgtc ctctcctgat
     3121 cccacggtgg tggggcagtt gaccacggtg acggcttcgg tggtgtcggt cgcgccgggg
     3181 tcgggggcgc cgtcgggtac ggtcacgttc catttcggtg atggcagtgc gacgttgacg
     3241 gcgccggtca gtaatggtct ggcggtggtg acgcacgctt atgggagtac ggcgggcagt
     3301 ccgttcgcga tcactgcgat gtatgacggg gatgcgaact tctcctcgtc gagcgggagt
     3361 gacacccaga ccgtcggtaa ggcggccacc accacggcgg tcatctcctc gccggatccc
     3421 acggtcgtcg gccagcccac caccctcacg gcctccgtcg cgtccgtggt gcccggggcc
     3481 gccgccccca ccggtacggt caccttcagc ttcggcgacg gcaccccctc gaccaccacc
     3541 acgctctccg acggcatcgc gaccgtcacg cacacctaca ccaccaggtc cggcagcccc
     3601 ttccccatta cggcgaccta caacggggat gccggcttca cgtcgtcgag tggcagtgat
     3661 gtgcagacgg tgggccgggc ggcgacgacg acggcggtgg tttcttcgcc ggatccgtcg
     3721 gtggcggggc aggcggtgac gttgaccgcg acggttgctg cggtggcgcc gggggccggt
     3781 actcctacgg gtacggtcac cttcagtttc ggtgatggca ccagcgccgc caccgccacc
     3841 ctctccggcg gcaccgcgac cgtgacgcac accttcagcg ggacctccgg cagcccgtac
     3901 accgtcacgg ccacctacaa cggcgacggc gacttcacct cgtcgagtgg cagtgatgtg
     3961 cagacggtgg gccgggcggc gacgacgacg gcggtggttt cttcgccgga tccgtcggtg
     4021 gcggggcagg cggtgacgtt gaccgcgacg gttgctgcgg tggcgccggg ggccggtact
     4081 cctacgggta cggtcacctt cagtttcggt gatggcacca gcgccggcac caccaccctc
     4141 tccggcggca ccgcgaccgt cacccacacc tacgccacca ggtccggcag ccccttcccc
     4201 gtcacggcca cctacaacgg ggacgccaac ttcgcgccct ccaccggcga tgacacccag
     4261 acgctcacca agaatgcgac caccaccacg gtgacctcct ctcccgaccc ctcggtgtcc
     4321 ggcgggacgg tgaccgtccg ggcgacggtc tcttccgctt ccggtacgcc gaccggcacg
     4381 gtcaccttca gtttcggtga cggcaccagc accgccaccg cgaccctctc cggcggagtc
     4441 gccaccgtca cccacgccta tacgaccacc accgggagcc cgttcaccct cacggccacc
     4501 tacggcgggg acgccagctt caacggctcc accggcaccg acacccagac cgtcacccgg
     4561 gccacgacca ccaccaccgt ggtctccacg cccaaccccg ccacgacggg tgaccggatc
     4621 acggtcaccg cgaccgtggt tcccgttgcc cccggaacgg gcacgcctac cggaaccgtc
     4681 accctcgcca tcacgggacg cagccccctg accgtgcctc tcgtcggcgg cacggccagt
     4741 acgtcgttca acccgctgtc gaagggctcc cacgtggtca ccggcaacta caacggcgac
     4801 gtcagcttcg cgccgtccac cggaaccact gtccagacgg tgaactgacg gagcggtccg
     4861 gttggcgcgt ccgttactta cccgcgcccg gggagtcgtg aagaatccgg acgcgacgac
     4921 gggcgctgaa tccaggggag ttgcgaccgg atcaatgcgt cacccttggc agcgtcagtg
     4981 caatgtgaaa tattactgac gtgcccacga gaagaagctc ggatgtcatc gtcgtcggag
     5041 cgggcgtggt cggcgctgcc tgcgcctact acgcggcccg cgccggcctc tccgtcaccg
     5101 tgatcgaccg cggccccgtc gcgggcggca ccacgggagc cggggagggc aacctcctgg
     5161 tctccgacaa ggagcccggc cccgaactcg atctcgcgct gctgtccacg gggttgtggc
     5221 aggaactggc ggaactgctc ccgccgcaga tcgagttcga gcccaagggc ggcctcgtgg
     5281 tcgccgccga cgagaccggg atgcgggccc tgcgggactt cgcggccgga cagggcaagg
     5341 ccggtgtgcg ggcggaggag gtccccgcgg accgcttgcg ggagctggaa ccgcatctga
     5401 catccggcct ggcagggggc ttccactatc cgcaggacgc tcaggtgatg ccggccctgg
     5461 ccgccgccca tctcctgcgg gcgggcggtg agcgggtgcg cctgaggctc ggtgaggagg
     5521 tcaccggcct gctgaccacg gcggacgggg cggtgcgggg ggtgcgtacg gcggccggcg
     5581 aggtctctgc cccgtacgtg gtcaatgcgg ccggcacctg gggcggcgag ttcgcccggc
     5641 tcgcgggcgt cgaactcccg gtgctgccgc gccgcggctt cgtgctcgtc accgaaccgc
     5701 tgccacgggt ggtacggcgc aaggtgtacg cggccgacta cgtggccgac gtcgccagcg
     5761 gctcggcggc actccagacc tcggcggtgg tcgagggcac cgctgccgga cccgttctga
     5821 tcggcgccag ccgggaacgg gtcggcttcg accggacggt gtcgaccgag gtgttgcgcc
     5881 gcctggcggc ccaggccacc gccctgttcc cggcgctcgc cggggtccgc gcgatgcgga
     5941 cgtatgtggg cttccggccg tacctgcccg accatctgcc ggccatcggc cccgatccgc
     6001 gggtgcccgg tctgctgcac gcctgcggtc acgaaggcgc gggaatcggc ctcgccccgg
     6061 ccaccgggct gctcatcgcg cggtccctga gcgggggcga accgcccctg gacccgggcc
     6121 cgttcagccc cgcccgcttc gaagccgccg cctgacccgt ccgctacgag aggaggcccg
     6181 cagtggcccg tacccccgcc gaactggcag gggcgaaccc cggcccgccg ttcgagatca
     6241 cgatggacgg ccgtccggtc cccgccctgc ccggccagtc cgtcgccgcg gccctgtggt
     6301 ccgccggcat cctcgcctgg cgcaccaccc gcaccaccgg ccgtccgcgg ggtgccttct
     6361 gcggtatcgg gcagtgctac gactgcctcg ccaccatcaa cggggagccc aaccgccggg
     6421 cctgtctggt gcccgcaagc cccggcgacg tgatcaccac ccaggaggga cacggccatg
     6481 cccgcatcgg cgtctgaccc gtacgacctc gcggtgatcg gcgcgggggc cgccggtctc
     6541 gccggcgccg tcacggcagc cgaactcggt ctgacggtgg ccctgttgga cgccgcaccg
     6601 cagaccggcg gccagttcta ccggcatccg gcaccggccg tgggcgcggc ccggcccgaa
     6661 gccttgcacc atgactgggc ggccttcgcc gaactgcgcc ggcgcctgga gcggagcgat
     6721 gtccgccggc tgtccgggca ccatgtctgg gccgtcacga aggaggacgg tgacctgtgg
     6781 gcggtgcacg ccgtcaccgg gcccgacggc accggggagc aatcggtccg ggtgcgggcc
     6841 cgtacggtcc tgttcgcgac cggcgcctat gaacgccaac tcccctttcc cggctggacc
     6901 gtaccggggg tcgtgggcgc cgggggagcg caggccatgc tcaagtcggg cttggtgctg
     6961 cccggtaagc ggatcgtcgt ggcgggcagc gggcccctgc tgctcgcggt cgccgcgtcg
     7021 ctggccgccg ccggtgcgca ggttcccgcc gtgatcgagg cgtccggcta tctggggtac
     7081 gcacgacacc cccgtgcgct cgccgccaat ccgcacaagc tcgtggaggc ggcggtccac
     7141 ggggccgccc tgctgcggca ccgggtccgg ctgcgcaccc gcagtgcggt caccgaggtg
     7201 cacggcaccg accgggtggc ggccgtcacc gtcacccggc tcgaccgcga ctggcggccc
     7261 gtgcggggga cgggccggcg gatcgcgtgc gacgcgctcg cggtgggcca cggcctggtc
     7321 ccgcagatcg aactggccac cgcactcggc tgcgccaccc gccgcaccgc ggacggaaca
     7381 tccggcctgg ccctgaccgg ccttcaggaa acctccgtac gcgggctgtg ggcggcgggg
     7441 gagaccgggg gagtgggcgg cgccccgctc gcccgggtgg aaggggaact ggccgggctc
     7501 gcggcagccg cccggctcca ggggcgcccg gccgacgaag ggcgcgtacg ggcgctgcgg
     7561 cgtcggcagg accggatgcg tgccttcgcc gaggcgatgg ccgtcagcca tgcaccggcg
     7621 gcgggctgga cgggctggct gaccgatgac acggaggtct gccgctgcga ggaagtgacc
     7681 gccggaacga tccgcgaggc cgtcaccggc ctcggcgccc gtgacgcccg caccgtcaaa
     7741 ctcctcaccc gggccggtat gggctggtgc cagggacgga tgtgcgggac ggcagcggcg
     7801 tgcctcgcgg caggtgacgg agcggcggaa ccactcgccg aacgccgtcc gttcgcggta
     7861 cccgttccgc tctcggcgct cgcctcggcc ggcgaccccg ccggaagccg gccggacgcc
     7921 gccccgcccg tcgacgactg accgcccgtc gcccttgtgg cggccgcgga cgacccaata
     7981 aaatgtcaca cgctacagaa gggttcaccg gcatgaccgc cctcacctgg aacagcaccc
     8041 gcccctggcg cggcatcatg gtcgccacgg cgctgccgct gcgcgacgat ctgtccgtcg
     8101 accatgacgc ctatgcccag cacgtccgtt ggctgatcga gcagggctgc gacggcgtcg
     8161 taccgaacgg ctcgctcggc gagtaccaga ccctcaccga cgacgagcgg gcccgcgtgg
     8221 tgcggaccgc ggtcgaagcg gcgggcgacg gcgcgcgggt catgcccggc gtcgccgcct
     8281 acggcagcgc cgagtcccgc cgctgggcgg agcaggcggg cgaggcgggc tgcggctcgg
     8341 tcctgctgct gcccccgaac gcctaccgcg ccgacgaacc ggccgtccgc gcccactacg
     8401 ccgaggccgc caaggcgggg gtgccggtcg tcgcgtacaa caaccccctc gacaccaagg
     8461 tggatctgac acccgggctg ctggcccggc tgcaccagga cggcagcatc gtcgcggtca
     8521 aggaattctc cggcgacgta cggcgcgcct acgagatcgc cgaactcgcc ccggaactcg
     8581 acctgttgat cggcgcggac gatgtcctgc tggaactcgc cgtggccggt gcggtcggct
     8641 gggtcgccgg ctaccccaac gccttccccg ccacctgcgc cgcgctgtac cacgccgccg
     8701 tcgccggtga cctggacacc gccctgccgc tctacaagtc cctgcacccg ctgctgcgct
     8761 gggactcgaa gacggagttc gtccagtgca tcaagctgtc catggacctc gtcggccgcc
     8821 acggtggccc cgcccgcccg ccgcgctccc cgctcaccgg ggagatcgag gccgaagtcc
     8881 gggccgccac cgagaaggcc gtcgccgccg gacaccactg accgcatccg tgccgcttcg
     8941 aaaggactca tggtgcgtac gcgtcatgtc ttccacgccg tcgactcgca caccgagggc
     9001 atgcccacgc gggtgatcac cggtggcgtc ggggtgctgc ccggcgccac catggccgag
     9061 cgcagactcc acttcatcga gcacctcgac cacctcagga ccctcctgat gtacgagccg
     9121 cgggggcact ccgccatgag cggcgccatc ctccagccgc ccacccgcgc cgacgccgac
     9181 tacggcgtcc tctacatcga ggtctccggt ctgctgccga tgtgcgggca cggcaccatc
     9241 ggggtcgcca ctgtcctcgt cgagtcgggc atggtgccgg tgaccgagcc ggtcactccg
     9301 gtgcggctcg acaccccggc cggtctggtc gccgtcgacg tacacgtggc ggacggggtg
     9361 gcgaagtcgg tcaccctcac caatgtgccc gccttctgcg ccggcctgga ccggaccgtg
     9421 gaggtcccgg gattcgggag ggtcacctac gacctcgcct tcggcggcaa cttctacgcc
     9481 ttcgtcgaac tcgacgcgct ggggctgccg ttcgaccggg cccgcaagga cgatctgctg
     9541 gccaccggac tggccgtcat gacggcgatc aacgcctcgc cggaccgtcc ggtccacccc
     9601 gagcagccgg agatcgccgg cgtcaagcat gtctacctgg ccgcccccgg ctcggacgcc
     9661 cagcactcgc ggcacgcgat ggcgatccat ccgggctggt tcgaccgctc gccctgcggc
     9721 accggcacct cggcgcggat ggcgcagctg cacgcccgcg gtgaactgcc cctgaaccgg
     9781 gacttcgtca acgagtcctt catcggcacc cgtttcaccg gccggctggt cgccgagacc
     9841 gaggtcggcg gacggcccgc cgtggtgccg accgtcaccg gccgcgcctg gatcaccggc
     9901 accgcccagt acttcctcga cccggacgac cccttccccg gaggcttcct cctgtgacca
     9961 ccgccgtcgc ttccgtcacc tcccgcaatc cggccgatcc gtccgatgtc ctggtgtcgg
    10021 ttccggcccc cggagccttc gccgcggcgg atgccgtcga gcgggcccgc gcggcccagc
    10081 ccgcctggct ggccggtggt gccgccgccc gctcggccgc cctgggcgcg gtcgccaccg
    10141 cccttgaggc cgccgcggac gaactggccg cgctcgccgt acgcgaagtg ggcaagccgc
    10201 tcaccgaggc gcgcgccgag atcgccagaa gcgtcgcgat ctggcgctac tacgcccagg
    10261 ccccctacga acccctcggt gccgtgcacg agaccgccgt cggcccgggg ctgctgctga
    10321 cccgccgccg tccttacggg gtcgccggcc tcctcacccc ctggaacttc ccgttcgcca
    10381 ttccgagctg gaaggcggct ccggcactcg ccgcgggcaa cgcggtggtc ctcaagcccg
    10441 cccccgaggc gacggcctgc gcccggcgcc tcgcctccct cgtcgagcag gccctgccga
    10501 ccggggtgtg cacggtgctg cccggcggag cgaccgaggg caacgcactc gtctccgtcg
    10561 ccgacgtcat ctccttcacc ggctcgacga ccgtcggccg cgcggtcgtg caggccgcca
    10621 cggcccgggg cgtcccggtc caggccgaga tgggcggtct gaacgcggcc ctggtcctcc
    10681 cggacgcgga catcgagcag gccgcgcgcc atatcgccac cgcgatcgcc ggatacgcgg
    10741 gccagaagtg caccgccacc cgtcgtgtga tcgcggtcgg accggcgctc ggccccctgc
    10801 gggaagcgct cgcggaagcc ctgcgtgcgg tgccggtcgg tgacccggcc gaccccgcca
    10861 ccgtgtgcgg gcccctcatc aacgagcagg cccgcgaccg ggtcacccgc gcccgggacg
    10921 gcctctcggt cctggcgggc ggaaccgcgc ccggcctccc cggctggtac gcggccccta
    10981 ccctggtcga gcaggtgccc ccgggccatc agctgctgcg cgaggaggtc ttcggaccgg
    11041 tcgcggcgct gctgcccgcc gacgacctgg cccatgcggt ccggatctcc aactcggtcc
    11101 cgtacggcct ggtcacctcg gtccactcgg ccggtctgga tccggtcctg cacgggctcg
    11161 accagctcga caccggaatg atcagagtca acgccccgtc caccggagtc gacttccatc
    11221 tgccgttcgg cggcgccaag gaatccggcc acggaccgcg ggaacagggc cgcgccgccc
    11281 tggagttcta cacctccagc cgtacgtaca ccctctccac ggtgggagca atgtgaaatt
    11341 gtatgctggt gatccgctcc gccaggaagg gatcaccacc gccatggggc acctgaagca
    11401 ccgcgatctc aacgccaccc gggagcggct gcgcgaccag gtcgcccatg ccctgcgcgc
    11461 cgccctgatc tccggtgaac tgcggccggg ggaggtgtac tcggcgccgg tgctggccga
    11521 ggacttcggg atctcggcca cgcccgtgcg cgaggcgatg ctcgacctgg cgcgcgaggg
    11581 cctggtcgag cccgtccgca acaagggctt ccgggtcacc gaagtcaatg agcgcgacct
    11641 cgaccagtac accgagatcc gcgcactcat cgagattccc atggtcgggc ggatcacccg
    11701 ctccgccgcc cgcgcggagc tggaggcgct gcggccggtc gccgaggaga tcgtgcgcgc
    11761 cgcgcgcgac cacgacctca tcggctacct ggaggccgac cggcagttcc acctcgaact
    11821 gctcggcctc agcggcaatg accgcctcgt cgagaccgtc ggtgacctgc gcaagcgctc
    11881 acggctctac gggctcacgg ccctggacga gcggggcgag ctgatcccgt cggccgagga
    11941 acacctcgaa ctcctcgacc tgatgctggc gggcgaggcg aaggccgccg agaagtgcat
    12001 ggccaagcac ctgggccatg tccgctcgct ctgggccagg agcgggaccg cggcgaaatc
    12061 cgccacgtag cgggccgacg gcagcgggcc ctgtcccctt acggacagcg acgccgtccg
    12121 ccctcccgcc ggtccatgcg atgtcacgct tcatgccgcc cccgggctgt ggaagggacc
    12181 catgaacccc gcatacacca ccgtcgacca cctcttcacc gtccccctgg accactccgc
    12241 ccccaccggg gaacacatac aggtcttcgc ccgcgaggtc gtcgacccgg tccgtgcgga
    12301 cgagcggctg ccctggctgc tgtacttgca aggcggtccg ggcggcaaat ccccccgccc
    12361 atcggccggc tcacccggct ggctggacca ggcactccag acccaccggg tgctcctgct
    12421 cgaccagcgc ggcaccggcc gttccacccc ggtcaccgcc cgagccgccg cccgcttcgc
    12481 ctcacccgcc caactcgccg cctacctggg ccacttccgc gccgactcga tagtggccga
    12541 cgccgaactg atccgccggc aactctgcgc ggacgaaccc tgggagaccc tcggccagag
    12601 ctacggcggc ttcctcaccc tcacctacct ctcccgcgcc cccgagggcc tgcgggcctg
    12661 ctacgtgacc ggtggtctgc ccggtctcac cgccaccgcc gacgaggtct acgcccgcac
    12721 ctacccgcgg gtgcgggacc gcgtcctcga cttctacgcc cgctacccgg acgacgcccg
    12781 ccgcctccgc cggatcgccg atctcctcac ggccaccgag gtccggctgc ccgacggcga
    12841 ccgcctcacc acccgccggc tgcgcaccct cggcctcgcc ctcggtatgg gcgacggctt
    12901 cgagcggctc cactggctgc tcgatgaatc cctggacgcc g
//
