LOCUS       NZ_BBNO01000007        10984 bp    DNA     linear   CON 19-AUG-2024
DEFINITION  Streptomyces lydicamycinicus strain TP-A0598, whole genome shotgun
            sequence.
ACCESSION   NZ_BBNO01000007
VERSION     NZ_BBNO01000007.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces lydicamycinicus
  ORGANISM  Streptomyces lydicamycinicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Komaki,H., Hosoyama,A., Igarashi,Y. and Tamura,T.
  TITLE     Streptomyces lydicamycinicus sp. nov. and Its Secondary Metabolite
            Biosynthetic Gene Clusters for Polyketide and Nonribosomal Peptide
            Compounds
  JOURNAL   Microorganisms 8 (3), 370 (2020)
   PUBMED   32155704
  REMARK    DOI:10.3390/microorganisms8030370 Publication Status: Online-Only
REFERENCE   2
  AUTHORS   Komaki,H., Ichikawa,N., Hosoyama,A., Fujita,N. and Igarashi,Y.
  TITLE     Draft genome sequence of marine-derived Streptomyces sp. TP-A0598, a
            producer of anti-MRSA antibiotic lydicamycins
  JOURNAL   Stand Genomic Sci 10, 58 (2015)
   PUBMED   26380643
  REMARK    DOI:10.1186/s40793-015-0046-5 Publication Status: Online-Only
REFERENCE   3  (bases 1 to 900839)
  AUTHORS   Hosoyama,A., Kimura,A., Ohji,S., Ichikawa,N., Yamazoe,A. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2014) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Newbler v. 2.6
            Genome Coverage                   :: 93x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina HiSeq 1000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000829715.2-RS_2024_08_19
            Annotation Date                   :: 08/19/2024 02:08:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,323
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,092
            CDSs (with protein)               :: 7,092
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 4, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 54 of 145
            Pseudo Genes (incomplete)         :: 123 of 145
            Pseudo Genes (internal stop)      :: 9 of 145
            Pseudo Genes (multiple problems)  :: 39 of 145
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:07:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 444233
            Orig. end                         :: 455217
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBNO01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10984
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="4"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10984
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10984
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5984](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5984)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            327..554
                     /locus_tag="TU31_RS24495"
                     /old_locus_tag="TPA0598_07_03990"
     CDS             327..554
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608014.1"
                     /locus_tag="TU31_RS24495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_03990"
                     /product="hypothetical protein"
                     /protein_id="WP_042158238.1"
                     /transl_table=11
                     /translation="MTEHPPVIVHPPTPSGGRRVTVRGQIVGLAHGRGDVAEFLRRAGV
                     TGPAGSIALDDPRLVEWRGGSLDDWPMPPA"
     gene            686..853
                     /locus_tag="TU31_RS24500"
                     /old_locus_tag="TPA0598_07_04000"
     CDS             686..853
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019548361.1"
                     /locus_tag="TU31_RS24500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_04000"
                     /product="hypothetical protein"
                     /protein_id="WP_245698675.1"
                     /transl_table=11
                     /translation="MSDDSGNSGSSDDGVFSTRSGGSGGSSDLSVLRRAMRWETLVILL
                     ILVILTVVAS"
     gene            1187..2707
                     /locus_tag="TU31_RS24505"
                     /old_locus_tag="TPA0598_07_04010"
     CDS             1187..2707
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092055.1"
                     /locus_tag="TU31_RS24505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_04010"
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="WP_042158240.1"
                     /transl_table=11
                     /translation="MNEDSRYEAVRSRDARFDGAFFFAVSTTGIYCRPSCPATTPRRRN
                     VAFFPTAAAAQGSGFRACRRCRPDAVPGSAEWNARADVVGRAMRLISEGVVDREGVAGL
                     AARLGYSARQVQRQLNAELGAGPVALARAQRAHTARVLLQTTGLPAAEIAFAAGFASVR
                     QFNDTIKEIYALTPSELRAARPGKGTRFGPVAPPTTPGVLPLRLAFRGPYAARQLFDHL
                     QRRAITGIEEVSGEPGARTYRRTLRLPHGAGIAEVDEAAGDGWLDCRLHLAELRDLTTA
                     SQRVRRLFDLDADPYAVAERLRADAVLARLVDRHPGLRSPGAPAPDELAVRAVLGQQVS
                     VAAGRTLGDALVAAYGEPLPEPAGALTHLFPRVDDLAQAALTELGMPESRRATLRTLST
                     ALADGTVVLDAGADRDEAERALLGLRGIGPWTAGYIRMRALGDPDVLLVGDAAVLAGMR
                     RAGAPTAGLRERANAWRPWRSYAMHHFWNAPVTEPASPRPTTTESTRP"
     gene            2704..3216
                     /locus_tag="TU31_RS24510"
                     /old_locus_tag="TPA0598_07_04020"
     CDS             2704..3216
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608011.1"
                     /locus_tag="TU31_RS24510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_04020"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_042158241.1"
                     /transl_table=11
                     /translation="MTLYTTLDSPLGELLLVGEESATAPGGTALASLSVPGQKGGATVQ
                     DGWTYAPTAFAGIAHQLRAYFDGELTRFALAYAPSRGTDFQQRVWQALEAIPYGTTTTY
                     GKIAADIGAARGAVRAIGTAIGANPLLVVRPCHRVIAANGSLSGYAGGPARKQQLLGIE
                     GARPELR"
     gene            3268..4050
                     /locus_tag="TU31_RS24515"
                     /old_locus_tag="TPA0598_07_04030"
     CDS             3268..4050
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092053.1"
                     /locus_tag="TU31_RS24515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_04030"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_181018653.1"
                     /transl_table=11
                     /translation="MTTTTTATTERHTRPGTLARRVTAADWTALAEELNTLGCAVTPRL
                     LTPADCRALSALYDGSAAVDGTDPASRARFRSTIDMARHRFGSGQYRYFADPLPTPVQA
                     LREAFYPHLREIARDWADKLGRPAPWPDTLEEWLTMCHEAGQTKSSQILLRYGAGDWNA
                     LHRDIFGEMVFPLQVVIGLDEQGTDYTGGEFLLVEQRPRAQSRGTSTVLPQGHGLIFTT
                     RDRPVRSARGWSAGPVRHGVSTVRSGRRHSLGLVFHDA"
     gene            complement(4091..4855)
                     /locus_tag="TU31_RS24520"
                     /old_locus_tag="TPA0598_07_04040"
     CDS             complement(4091..4855)
                     /NRPS_PKS="Domain: PKS_KR (7-176). E-value: 2.1e-10. Score:
                     32.7. Matches aSDomain:
                     nrpspksdomains_TU31_RS24520_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="TU31_RS24520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_04040"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_042158242.1"
                     /sec_met_domain="adh_short (E-value: 5.2e-30, bitscore:
                     100.4, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGELKGKTALVTGSSRGIGRGIAQRLAADGALVAVHYRSNDTAAK
                     ETAESILDAGGRAFIIGAELGVPGDAEALYAAFDEGLAAYGAEPGLDILVNNAAISIPG
                     HIDEVTPEDFDRTLAVNTKAVFFIAQHGLRRMRDGGRIINVSSAVTSTAFPSTIAYSVS
                     KGAVDAFTLTLAKDLGARGITVNTLAPGFIETDMNASMRATPEAHAALASLSVFNRIGQ
                     PQDIADVAAFLASDESRWITGQRIDVTGGSML"
     aSDomain        complement(4328..4834)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TU31_RS24520_PKS_KR.1"
                     /evalue="2.10E-10"
                     /label="TU31_RS24520_PKS_KR.1"
                     /locus_tag="TU31_RS24520"
                     /protein_end="176"
                     /protein_start="7"
                     /score="32.7"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGIGRGIAQRLAADGALVAVHYRSNDTAAKETAESIL
                     DAGGRAFIIGAELGVPGDAEALYAAFDEGLAAYGAEPGLDILVNNAAISIPGHIDEVTP
                     EDFDRTLAVNTKAVFFIAQHGLRRMRDGGRIINVSSAVTSTAFPSTIAYSVSKGAVDAF
                     TLTLAK"
     CDS_motif       complement(4763..4828)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_TU31_RS24520_0001"
                     /evalue="3.00E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="TU31_RS24520"
                     /protein_end="31"
                     /protein_start="9"
                     /score="10.3"
                     /tool="antismash"
                     /translation="LVTGSSRGIGRGIAQRLAADGA"
     gene            complement(5001..5984)
                     /locus_tag="TU31_RS24525"
                     /old_locus_tag="TPA0598_07_04050"
     CDS             complement(5001..5984)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="TU31_RS24525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_04050"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_078885933.1"
                     /sec_met_domain="AfsA (E-value: 1.9e-19, bitscore: 65.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTMQVSTVQPRSPHQLPAPVGDRATGPRSPHGAQVPQEFVHRP
                     LADDMLVTSWRRVGDTRFSLTAQWPHDHGYFTPNRGRHHLILTGETIRQAGLLLCHAEL
                     GVPLGHHFILGDLVYTTHPEHLAVGSGPTPLTIDVLCSRTRMRAGTLTSGRFDMTFRKA
                     GRIVATGHSDVTVTSPAVYRRLRGDRLSARHTPGPLPAPVPPRPTGSAKEHDVLLSPTG
                     RPDRWLLRIAPGHPAVVNPANDHVPGMVLLDAAQQAARTLTSPGAFVPYAFGTVFHRYA
                     EHGTPCVIEAHRVPSALPCTTTVQVTGLQNGSPVFVSTLTALDPRD"
     misc_feature    complement(6046..6059)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.3"
                     /tool="antismash"
     gene            6159..6845
                     /locus_tag="TU31_RS24530"
                     /old_locus_tag="TPA0598_07_04060"
     CDS             6159..6845
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032559.1"
                     /locus_tag="TU31_RS24530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_04060"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_042158244.1"
                     /transl_table=11
                     /translation="MAQQDRAIRTRRVILEAAASVFDEQGYDRATIAEVLERAGVTKGA
                     LYFHFASKEQLALAVIEEQVVDIAVQPQTIKLQEFVDSGQVLAFRLRSDPIQRGAARLA
                     VEQGSNHLDRKQSMLSWTRFVEGLLNDARSRGEILESVVVGETAELFVGAFAGLQMMSQ
                     ALTNRADLSRRLTVFFEHTLPSIAVPAILAKLNLDSERGAQLDAALQAKAVVKVAADAD
                     AGVAAG"
     misc_feature    complement(6890..6906)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.09"
                     /tool="antismash"
     misc_feature    complement(7200..7215)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     gene            7520..10207
                     /locus_tag="TU31_RS40465"
                     /old_locus_tag="TPA0598_07_04070"
     CDS             7520..10207
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574653.1"
                     /locus_tag="TU31_RS40465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_04070"
                     /product="MFS transporter"
                     /protein_id="WP_042158245.1"
                     /transl_table=11
                     /translation="MTRTTTDQLPARAAEIAATPPGSDPAVGGRRRSAGGIVPVLAFAG
                     IVVAVMQTLLVPVIKDLPLLLNTAPSNATWVMTATLLAGAVSTPIMGRLGDLYGKRRML
                     LTSLAVMVVGSLVCGFTSDLVTMIVGRALQGFAMGAIPLGIGIMRDELPRERLGSAMGL
                     MSSSIGVGGGLALPAAALVAQHADWHTLFFGAAGLGVLSMVLTLLVVPETAVRAQGRFD
                     VAGALGLSAGLVALLLPLTKGSDWGWGSPTTLILFGVAVVILVLWGAMELRLADPLVDL
                     RTTARREVLLTNLASITVGVAFYAISLVLPQLLQLPTSTGYGLGQSMVVAGLCMAPLGL
                     TMMFVAPLYARIAARRGPKVSLLLGMLVIALGYGAGIGLMNAPWQTVIIAVVVGAGIGL
                     AYSSLPALIIGAVDASETGAANGLNTLMRSIGTSVSSAVIGMVLADMSQKTGPVTVPTM
                     AGFRVSFLIATAAVLVGVALACFLPSQRKASRPTLLAQSTDGAADAVDADGTRGAARPP
                     AMPAAPLTATPPVHAAPAAADDAAESAVPAEAVEPAAPAKAAEPAAPAKAAEPAAPAKA
                     AEPAAPAKAVEPMAAGNAVALTAAEEQPAPLVKSAPSWARPVTEPDEVRTPPSSGATAA
                     SADPAGFRGRVADTSGGPVPGASITLIDRQGRQAGIATADADGHFAVDAPTAGTYVLTG
                     AAPGHTPHAASATYRGEGAATRLDLVLAGTGRLGGTLLGGTEGTPLAGGSIVVTDAEGA
                     VVARTASGAEGSWDVAPLPPGHYTLVLSAAGHQPQARAVDLSGSAPERQDARLKPAATV
                     RGTVRGPNGRPLAEAAVTLVEDDAIAGHTVTGPDGAFAFSGMSGAHYTLTAAGYPPHAV
                     PISLTAGAHETLDLDLSQPPTAGG"
     gene            complement(10375..10959)
                     /locus_tag="TU31_RS24540"
                     /old_locus_tag="TPA0598_07_04080"
     CDS             complement(10375..10959)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1298: putative carboxymuconolactone decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528860.1"
                     /locus_tag="TU31_RS24540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_04080"
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_042158246.1"
                     /transl_table=11
                     /translation="MPARIAPAEAPHSSEVAAVLHRMMPGDEEPIALFRLYARNLPLAE
                     ALHAWGSYQLSRQLSLGLRDREILIDRTCARCGCEYEWGVHIARFAPRVGLTPAHISSL
                     THGNSADACWTTDRDRLLLDAADALHTGHDIDDALWERLSAEFSPEQLLDLLMLCGWYH
                     AISFTARAGRLTPEPGTPKFDDFLPPAAPKP"
ORIGIN
        1 ggtgtggctg tcgaactcct tcaccagccc catgtcgtgg aacatggccg acacatagag
       61 gagctccgga tcgaaggcga tgtcgtgggc cgaggcgtag gcggccgccc agagatacgc
      121 ccgcacggag tggttcagca gcgcgggcga gcagtaggcg ctcgccacct ccagcgcggc
      181 acgacaggcg cgggtatcgg gaatcacaag atcatccatg gtcatggcca tgattctggc
      241 gcatccccct gtgcccgtcg cggaggattg acgctcacgg cggctgccga aatgcgcgag
      301 cgcggacccc ggcgcacgct ggatgcatga ccgagcaccc gcctgtcatc gtgcacccgc
      361 cgacgccgtc cggtggccga cgggtgaccg tgcgtgggca gatcgtcggc ctggcccatg
      421 gccgcggcga tgttgccgag ttcctccgtc gcgcgggcgt caccggcccg gccgggagta
      481 tcgcgctcga cgatccccgc ctcgtcgaat ggcgcggtgg ctcgctggac gactggccga
      541 tgccaccggc gtgacatccg ggccgatcag ccgttcgccg tggccgtacc cgtctctcgg
      601 tgccgcgggc ccgagggata cgctgacctc cggcgatcgg cagggccgtc ggtcggtgag
      661 ccggcggcag ggagcggagc agggcgtgag cgacgacagc gggaacagcg gcagcagcga
      721 cgacggcgtg ttctcgacgc gcagtggcgg gtcgggcggc tcttcggacc tgagtgtcct
      781 gcggagggcg atgcgctggg agactctcgt gatcctcctg attctcgtga tcctcacggt
      841 agtggcctcg taggacgggt tcgataccgg ggaatccctg aactcgcgtc ggcctcttgg
      901 gagttcaggt gctcacgtgc tgaccgggca cggtcccgga atcctccctg gcagggcccg
      961 tgggcctgaa ctcccttacc gttaaaggct gttgtccgcc gggccggcgg tgacccgcgg
     1021 tccggcggtg tgccgcgatc ctgtggggcg gcacggcctc aaggggggcg ggcgcccgca
     1081 cccccaagcg cgtacgcgtg gccccgtggg cccgccccgg ctccccgaac gcaccgtccg
     1141 atttccgcca gcaccgccgc ctgacctgct gcacagtgga accgtgatga acgaggacag
     1201 caggtacgag gcggtgcgca gcagggacgc ccgattcgac ggcgccttct tcttcgcggt
     1261 gtccacgacc gggatctact gccggccgag ctgtcccgcc accacgcccc ggcgccgtaa
     1321 tgtcgctttc ttcccgaccg ccgcggccgc ccagggctcc ggattccgcg cctgccggcg
     1381 ctgccgcccc gatgcggtgc ccggctcggc ggagtggaat gcccgcgccg atgtcgtcgg
     1441 gcgggcgatg cggctgatca gcgagggtgt ggtcgaccgg gaaggcgtcg ccgggctggc
     1501 ggcccggctc ggctacagcg cccggcaggt ccagcggcag ctgaacgccg agctgggcgc
     1561 cgggcccgtg gccctggcgc gggcacagcg cgcccacacc gcccgggtcc tgcttcagac
     1621 caccggactc ccggcggccg agatcgcctt cgcggcgggc ttcgccagcg tgcggcagtt
     1681 caacgacacc atcaaggaga tctacgcgct cactccgagc gaactgcgcg ccgcccgccc
     1741 cggcaagggg acccgcttcg gcccggtggc gccgcccacc acgcccgggg tgctgccgct
     1801 gcggctggcc ttccgcgggc cgtatgcggc gcggcagctc ttcgaccacc tccagcggcg
     1861 tgcgatcacc ggcatcgagg aggtcagcgg cgagcccggg gcccgtacct accggcggac
     1921 cctgaggctg ccgcacggcg ccgggatcgc cgaggtcgac gaggcggccg gggacggctg
     1981 gctggactgc cggctgcacc tggcggagct gcgggatctc accaccgcga gccagcgggt
     2041 gcgccgcctc ttcgatctgg acgccgatcc gtacgcggtc gccgagcggc tgcgcgcgga
     2101 tgccgtactg gcgcggctgg tggaccggca ccccgggctg cgctcgccgg gcgctcccgc
     2161 cccggacgag ctcgccgtcc gtgccgtcct cggccagcag gtctcggtcg cggccggccg
     2221 cacactgggc gacgccctgg tcgccgcgta cggagagccg ctgcccgaac cggccggtgc
     2281 gctcacccac ctcttccccc gcgtcgacga cctggcgcag gccgcgctca ccgaactcgg
     2341 gatgcccgaa tcccggcgcg cgaccctgcg aaccctgagc accgccctcg ccgacggcac
     2401 ggtcgtgctg gacgccggag ccgaccggga cgaggccgaa cgcgcgcttc tcggactgcg
     2461 cggcatcggc ccgtggaccg ccggatacat ccggatgcgc gccctcgggg accccgatgt
     2521 cctgctggtc ggcgatgcgg ccgtactggc cgggatgcgc cgggccggcg ctcccacggc
     2581 cggtctgcgg gagcgggcga acgcctggcg cccctggcgc tcgtacgcga tgcaccactt
     2641 ctggaacgcg ccggtcacgg agccggcgag cccccgcccg accaccacgg aaagcacccg
     2701 accatgacgc tctacaccac cctcgacagc ccgctgggcg aactcctgct ggtgggcgag
     2761 gaatcggcca ccgccccggg cggcaccgcg ctcgcttcgc tgtccgtacc cgggcagaag
     2821 ggcggggcga ccgtccagga cggctggacg tacgccccca ccgcctttgc cggcatcgcc
     2881 caccagctcc gcgcctactt cgacggggag ctgacccgct tcgcccttgc ctacgccccc
     2941 agccggggca ccgacttcca gcagcgcgtg tggcaggccc tggaggccat cccgtacggc
     3001 accaccacca catacgggaa gatcgccgcc gacatcggtg cggcgcgcgg tgcggtacgc
     3061 gccatcggaa cggcgatcgg cgcgaacccg ctgctcgtcg tccggccctg ccatcgggtc
     3121 atcgccgcga acggttccct gtcgggctac gcgggcgggc ccgcacgcaa gcagcagctc
     3181 ctcggcatcg agggcgctcg gccggagctc cgctgagctg cccccaccgc cgagcgccgc
     3241 accgacccac gcccactgga gcgctccatg accaccacga ccactgcgac caccgagcgc
     3301 cacacccgcc ccggcaccct cgcccggcgc gtcacggccg ccgactggac ggcgctggcc
     3361 gaggaactga acaccctggg ctgcgcggtg acgccacgtc tgctcactcc ggccgactgc
     3421 cgtgccctca gcgcgctcta cgacggatcc gccgcggtcg acggcacgga ccccgccagc
     3481 cgggcccgct tccgctcgac cattgacatg gcacggcacc gcttcggctc gggccagtac
     3541 cgctacttcg ccgacccgct gcccacacct gtccaggccc tgcgcgaggc gttctacccg
     3601 cacctgcggg agatcgcccg cgactgggcg gacaagctcg gcagaccggc cccctggccg
     3661 gacaccctgg aggagtggct gaccatgtgc cacgaagccg ggcagaccaa gtcctcgcag
     3721 attctgctgc gttacggcgc gggggactgg aacgcgctgc accgcgacat cttcggggag
     3781 atggtcttcc ccctccaggt cgtcatcggc ctggacgagc agggcaccga ctacaccggc
     3841 ggcgaattcc tcctcgtcga gcagcggccg cgcgcccagt cccgcggcac cagcaccgtc
     3901 ctgccacagg gccacggact gatcttcacg acccgcgacc gccccgtacg ctccgcccgg
     3961 ggctggtcgg ccggccccgt acgccacggt gtgagcaccg tccgctccgg ccggcggcac
     4021 tccctgggcc tggtcttcca cgacgcctga agcggctgcc gctgcacgcg ggcccagggg
     4081 gccgacggcc tcagagcatg gagccgccgg tgacatcgat gcgctggccg gtgatccagc
     4141 gggactcgtc ggacgcgagg aaggcggcga cgtcggcgat gtcctggggc tggccgatcc
     4201 ggttgaacac cgagagggag gccagcgcgg cgtgcgcctc cggagtcgcc cgcatcgacg
     4261 cgttcatatc ggtctcgatg aagccggggg cgagggtgtt caccgtgatg ccccgcgctc
     4321 cgagatcctt cgccaaggtg agggtgaagg cgtccaccgc gcccttggag acgctgtagg
     4381 cgatggtcga ggggaacgcc gtactcgtca ccgccgacga gacgttgatg atgcgtccgc
     4441 cgtcccgcat ccgccgcaga ccgtgctggg cgatgaagaa cacggccttg gtgttgaccg
     4501 cgagggtgcg gtcgaagtcc tcgggcgtca cctcgtcgat gtgccccggg atgctgatcg
     4561 ccgcgttgtt gacaaggatg tcgagaccgg gctcggcgcc gtacgccgcc agcccctcgt
     4621 cgaacgcagc gtagagcgcc tcggcatcac cgggaacacc cagttccgca ccgattatga
     4681 aggcccggcc gcccgcgtcg aggatgctct cggccgtttc cttggccgcg gtgtcattac
     4741 tgcggtagtg gacggcgacc agtgccccgt cggcggcgag gcgctgcgcg atgccccggc
     4801 cgataccccg gctggatccc gtcaccagcg cggtctttcc cttgagttca cccatggtgg
     4861 tctggccttt cacgtgccgg tggtgggcat accgcccccg aggactgtcg agtggtgtgg
     4921 tggtgacgtg gccggaccgg gccggctgct ccgcgtggtg cgtcggccga cggcgaacgg
     4981 tccgccgtga gaacgggtcg tcagtcccgt gggtccaacg ccgtcagggt ggagacgaag
     5041 accggactgc cgttctgcaa gccggtcacc tgcaccgtgg tggtgcaggg cagcgccgac
     5101 gggacgcggt gagcttcgat cacacagggg gtgccgtgct cggcgtaccg gtggaaaacg
     5161 gtcccgaagg cgtacgggac gaaggcgccg ggcgaggtga gggtgcgggc ggcctgctgg
     5221 gcggcgtcga gcagcaccat gccggggaca tggtcgttcg ccgggttcac caccgcgggg
     5281 tggccgggcg cgatgcgcag cagccagcgg tcgggccggc cggtggggga gagcaggacg
     5341 tcgtgttcct tggcgctgcc ggtcggccgg ggcggaacgg gggcggggag ggggccgggg
     5401 gtgtgccgcg cactcagtcg atcgccgcgg agccggcggt agacggcggg tgaggtgacc
     5461 gtgacgtcgg agtggccggt cgcgacgatc cggcccgcct tacggaaggt catgtcgaag
     5521 cggccactgg tcagcgtgcc cgcccgcata cgcgtccgac tgcagaggac gtcgatggtc
     5581 agtggcgtgg gcccgcttcc caccgcgaga tgctcggggt gggtggtgta cacgaggtcg
     5641 ccgaggatga agtggtggcc gagcgggaca ccgagttcgg cgtggcagag caacaacccg
     5701 gcctgacgga tggtctcacc cgtgaggatg agatggtgtc gcccgcgatt gggggtgaag
     5761 taaccgtgat cgtgcggcca ctgggcggtc agcgagaaac gcgtgtcacc gacccggcgc
     5821 catgaggtga ccagcatgtc gtcggcgaga gggcggtgta cgaactcctg ggggacctgg
     5881 gcgccgtggg ggctccgcgg tccggtcgcc cggtccccga cgggggccgg cagttggtgt
     5941 ggagacctgg gctgaacagt gctgacctgc atcgtgtcgc tcatgactac ccccaggcgt
     6001 cgctacgggt ggaattacat cgctgataag atacgtacca tccggtttta ttttcaatgc
     6061 cgggccgtcg aaaaggggaa agtagctgat cgagcgacga cttgagctgt agctaactgt
     6121 tgcggaacgt agagacatcg agagacagga ggcgtcctgt ggcacaacag gaccgcgcga
     6181 ttcggacccg gcgagtgatc ttggaggcgg ctgcgtcggt tttcgacgag cagggttacg
     6241 accgcgcgac gatcgccgaa gtcctcgagc gcgccggcgt gacgaagggc gccttgtact
     6301 tccacttcgc ctccaaggaa cagttggcgc tcgcggtgat cgaggagcag gtggtcgata
     6361 tcgccgtgca gccgcagacg atcaagctgc aggaattcgt cgactcgggc caggtgctgg
     6421 ccttccggct gcgcagcgac ccgatccagc ggggagcggc gcgactggcg gtggagcagg
     6481 gctccaatca cctcgaccgc aagcagtcga tgctgtcgtg gacccggttc gtcgagggct
     6541 tgctgaacga cgcccggagc cgcggagaaa ttctggagag cgttgtcgtg ggggagaccg
     6601 ccgagttgtt cgtcggcgcc ttcgcggggc tgcagatgat gtcgcaggcc ctgaccaacc
     6661 gggccgacct cagtcgtcgg ctcaccgtct tcttcgaaca caccctcccg agcatcgccg
     6721 tgccggccat cctcgccaaa ctcaacctgg actccgaacg gggtgcccaa ctcgatgccg
     6781 ccctgcaggc gaaggcggtg gtgaaggttg cggccgacgc cgatgccgga gtggccgccg
     6841 gctgatcccg ccccgtccga tgtgcagttg cggtcgcggg aggcggagga tgttttggtg
     6901 catcacgacc gctgaaaaca taccgcgtgt gcggtgttga atatcgaagt gtgtgcaatg
     6961 cgtgggtgag ctgtgcaggt caggggcgtc gcggaagagc cgcggcgcct ctcggcgttg
     7021 aggtggcggc aggatgtgcg ggagccgcga acggtggccg gcgaaggcgc ggcacttcgg
     7081 gctcgggtgg atgtcagtgg tgtgctgttc actggactcg gcgggctcgg agaggccgcg
     7141 tccgggacga acgggggagg tccggtgcac gggttgaggg agagcgaagg ccggggcggc
     7201 gtgacggaga tcaccgatgc cgggatcggg tgccgggtcg tacggcggaa tagaacgccg
     7261 gggtagaagc gttcgtcatg cacgtatcag ccgtgcgcgg agaactcccc actcgtcagc
     7321 ggcctgatca cgccgatgcc cgtagacgac accacacttc gaccgctcct ccgacgatcc
     7381 ctccgccgcc ctcgtgctgt gccgtcccat gtcccgacga cgctcacacc ccaggccgcg
     7441 tccgcctccc tctgctcacc cctgctccgt cacccgctga tgacatccat ccacctctgc
     7501 cgccctggag gccgcacgca tgacccggac gacgacggac caactccccg caagggccgc
     7561 ggagatcgcg gccacgcccc cggggtccga cccggccgtg ggcgggcggc ggagatctgc
     7621 cggcggcatc gtcccggtcc tcgccttcgc gggcatcgtc gtcgcggtga tgcagacgct
     7681 gctcgtgccg gtcatcaaag acctgccgtt gctgctcaac accgcgccca gcaacgccac
     7741 ttgggtcatg acggccaccc tcctcgccgg tgccgtctcg acccccatca tggggcgcct
     7801 cggcgatctg tacggcaagc ggcggatgct gctgaccagc ctcgcggtca tggtcgtcgg
     7861 ctcgctggtc tgcggcttca ccagtgatct ggtcaccatg atcgtgggcc gggcgctcca
     7921 gggcttcgcc atgggcgcca ttcctctcgg gatcggcatc atgcgcgacg agctgccgcg
     7981 cgagcggctc ggctcggcca tgggcctgat gagctcatcg atcggcgtcg gcggcgggct
     8041 cgccctgccg gccgcggccc tggtcgccca acacgccgac tggcacaccc tgttcttcgg
     8101 cgccgccggg ctcggcgtgc tgtcgatggt gctcaccctc ctcgtcgtcc cggagaccgc
     8161 ggtacgggcc caggggcgct tcgatgtggc gggcgccctc gggctgtcgg ccggtctggt
     8221 ggccctgctg ctgcccctca ccaagggcag cgactggggc tggggttcgc cgaccaccct
     8281 catactcttc ggtgttgcgg tcgtgatcct ggtgctgtgg ggcgcgatgg agctgcgtct
     8341 cgccgacccg ctggtcgatc tgcggaccac cgcgcgccgt gaggtgctgc tgaccaacct
     8401 cgcctcgata acggtcggtg tggcgttcta cgccatctcc ctggtgctgc cgcagctgct
     8461 ccagctgccc acgtcgaccg gctacgggct gggtcagtcg atggtggtgg ccgggctgtg
     8521 catggcgccc ctgggtctga cgatgatgtt cgtcgccccg ctgtacgccc ggatcgccgc
     8581 ccggcgcggc cccaaggtct cgctgctgct cggcatgctc gtcatcgcgc tcggctacgg
     8641 cgcgggcatc ggcctgatga acgccccctg gcagaccgtc atcatcgcgg tggtcgtcgg
     8701 cgccggtatc ggtctggcgt actcctcgct gcccgccctg atcatcgggg ccgtcgacgc
     8761 ctcggagacc ggtgcggcca acggcctgaa caccctgatg cgctccatcg gcacctcggt
     8821 gtccagcgcg gtgatcggca tggtcctggc cgatatgtcc cagaagacgg ggccggtcac
     8881 ggtgcccacg atggccggct tccgggtctc cttcctcatc gccacggccg ccgtcctcgt
     8941 cggcgtcgcg ctggcgtgtt tcctgccgtc acagcgcaag gcgtcccgcc cgaccctgct
     9001 cgcccagagc accgacggcg cggcggacgc ggtggacgcc gatggaacgc ggggtgcggc
     9061 gcgcccgccg gcgatgcccg ctgcgccgct caccgccaca ccgcccgtac acgctgcccc
     9121 cgccgcagcg gacgacgcag ccgaatcggc cgtcccggcc gaggcagttg aaccggccgc
     9181 cccggcgaaa gcagccgaac cggccgcccc ggcgaaagca gccgaaccgg ccgccccggc
     9241 gaaagcagcc gaaccggccg ccccggccaa agcagttgaa ccgatggccg cgggtaacgc
     9301 agtcgcactt accgccgcag aggagcagcc ggccccgctg gtgaagtcgg cgccgtcctg
     9361 ggcgcggccg gtcaccgaac cggacgaggt ccgcaccccg ccgtcctccg gggcgacggc
     9421 cgccagcgca gaccccgccg ggttccgggg ccgggtggcg gacacctccg gcggcccggt
     9481 ccccggcgcg agcatcaccc tgatcgaccg gcagggccgc caggccggca tcgccacggc
     9541 cgacgccgac ggtcacttcg cagtcgacgc ccccacggcc ggcacctatg tcctcaccgg
     9601 cgccgcgccc ggccacacac cccacgcggc ctccgccacc taccgcggcg aaggcgccgc
     9661 gacccggctc gacctcgtcc tcgccggcac cggccgactg ggcggcacct tgctcggcgg
     9721 caccgagggc accccgctgg ccggcgggag catcgtcgtc accgatgccg aaggcgctgt
     9781 ggtggcccgt acggcctccg gcgccgaggg gagctgggat gttgccccac tgccgcccgg
     9841 ccactacacc ctggtcctca gtgccgccgg tcaccagccg caggcccgtg ccgtggacct
     9901 gtccggcagc gcaccggaac gccaggacgc ccgcctgaag ccggcggcca ccgttcgcgg
     9961 gacggtacgg ggcccgaacg gccgaccgct cgccgaagct gccgtgaccc tcgtcgagga
    10021 cgacgcgatc gccggacaca ccgtcaccgg accggacggc gccttcgcct tctccggcat
    10081 gagcggcgcc cactacaccc tcacagcggc cggttaccca ccccacgccg tcccgatatc
    10141 cctcaccgcc ggcgcccacg aaaccctgga cctggacctg tcccagccac cgacagccgg
    10201 cggctgacgg cctccggcac gcggacgcac gacggcagac agacaccaac gccctgccga
    10261 gagccactca tccggcaggg cggcggctgc tcggccgcgg ccccaagtcg gcttctccgc
    10321 cgacgacctg gcacgcagcc gtccgccgcc ggccccgcct tgccgtggcg tgcttcaggg
    10381 cttcggtgcg gccggaggga gaaagtcgtc gaacttcggt gtgccgggtt ccggggtgag
    10441 cctgccggcg cgggcggtga aactgatggc gtggtaccag ccgcacagca tcagcaggtc
    10501 caggagctgt tccgggctga actccgcgct gagacgctcc cacagcgcgt catcgatgtc
    10561 gtggccggtg tgcagggcat cggccgcgtc gaggaggagc cggtcgcggt cggtggtcca
    10621 gcaggcgtcc gcgctgttgc cgtgcgtcag ggagctgatg tgcgccgggg tcagtccgac
    10681 gcggggtgcg aagcgcgcga tatgcacgcc ccattcgtac tcgcagccgc agcgggcgca
    10741 ggtgcggtcg atgaggatct cgcggtcccg caggccgagg ctgagttgcc ggctgagctg
    10801 gtacgagccc caggcgtgca gcgcctcggc cagcgggagg ttgcgtgcgt acagccggaa
    10861 gagcgcgatc ggctcctcgt cccccggcat catgcggtgc agtaccgccg ccacctctga
    10921 cgagtgcggc gcttcggccg gtgcaatccg ggcaggcacg actccccctt ccactgattc
    10981 gcgt
//
