LOCUS       NZ_BBNO01000007        11935 bp    DNA     linear   CON 19-AUG-2024
DEFINITION  Streptomyces lydicamycinicus strain TP-A0598, whole genome shotgun
            sequence.
ACCESSION   NZ_BBNO01000007
VERSION     NZ_BBNO01000007.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces lydicamycinicus
  ORGANISM  Streptomyces lydicamycinicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Komaki,H., Hosoyama,A., Igarashi,Y. and Tamura,T.
  TITLE     Streptomyces lydicamycinicus sp. nov. and Its Secondary Metabolite
            Biosynthetic Gene Clusters for Polyketide and Nonribosomal Peptide
            Compounds
  JOURNAL   Microorganisms 8 (3), 370 (2020)
   PUBMED   32155704
  REMARK    DOI:10.3390/microorganisms8030370 Publication Status: Online-Only
REFERENCE   2
  AUTHORS   Komaki,H., Ichikawa,N., Hosoyama,A., Fujita,N. and Igarashi,Y.
  TITLE     Draft genome sequence of marine-derived Streptomyces sp. TP-A0598, a
            producer of anti-MRSA antibiotic lydicamycins
  JOURNAL   Stand Genomic Sci 10, 58 (2015)
   PUBMED   26380643
  REMARK    DOI:10.1186/s40793-015-0046-5 Publication Status: Online-Only
REFERENCE   3  (bases 1 to 900839)
  AUTHORS   Hosoyama,A., Kimura,A., Ohji,S., Ichikawa,N., Yamazoe,A. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2014) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Newbler v. 2.6
            Genome Coverage                   :: 93x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina HiSeq 1000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000829715.2-RS_2024_08_19
            Annotation Date                   :: 08/19/2024 02:08:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,323
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,092
            CDSs (with protein)               :: 7,092
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 4, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 54 of 145
            Pseudo Genes (incomplete)         :: 123 of 145
            Pseudo Genes (internal stop)      :: 9 of 145
            Pseudo Genes (multiple problems)  :: 39 of 145
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:07:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 212326
            Orig. end                         :: 224261
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBNO01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11935
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11935
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11935
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6935](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6935
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            759..1136
                     /locus_tag="TU31_RS23445"
                     /old_locus_tag="TPA0598_07_01870"
     CDS             759..1136
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057039.1"
                     /locus_tag="TU31_RS23445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_01870"
                     /product="DUF2267 domain-containing protein"
                     /protein_id="WP_042158023.1"
                     /transl_table=11
                     /translation="MDDKEFFRAVADRARLSRQEGADLTRATLETLALRLSDGEARDLA
                     LELPEPLRVSLKRERREMEIFGPDESIRRVRARTGLSASEADRGVRAVLGTLQEVISRK
                     EFEHAMSQLGKEYAQLVEAAR"
     misc_feature    complement(1308..1322)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: medium, score: 22.51"
                     /tool="antismash"
     misc_feature    1397..1411
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.1"
                     /tool="antismash"
     gene            1622..2893
                     /locus_tag="TU31_RS23450"
                     /old_locus_tag="TPA0598_07_01880"
     CDS             1622..2893
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088740.1"
                     /locus_tag="TU31_RS23450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_01880"
                     /product="MFS transporter"
                     /protein_id="WP_042158024.1"
                     /transl_table=11
                     /translation="MTGTAPAVAPAAPLPHVRRDIRLFWWAGTCDALGSQTSGLVLPLL
                     LLTLGRSPAEVGALAGLSAVATLLLGPFAAVPADRGARKQLMVGSALVSAAAMAGVTVA
                     VAAGPVPLAVLIAAVLVERCATSCYEAAALGTVALVCPPGEHRRVLSRLQAGEGGALVL
                     GPALGGLLFHAERWLPFLADALSYAVAAGCIGAMRAPLVPPREQKSPAGAVRRGLRSRA
                     AEAAAGLALIRREPFLRLALLWISAVNALLVALYYTTVFALQHDGRGAAPLGLVLALAG
                     AAGLAGALAAPRIAGRRSAGRVLVTVSWLMVPPAAALAMARDAWHFGLLFGLLCLLTPV
                     ATVALQARIIQVVPPGLQARTGTVLATVSGATAAVAPALAGLSADRAGAAVTLMGCAAL
                     LAVLALHTTCVAPARTRRADSEPA"
     misc_feature    2078..2080
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2890..4740
                     /locus_tag="TU31_RS23455"
                     /old_locus_tag="TPA0598_07_01890"
     CDS             2890..4740
                     /NRPS_PKS="Domain: Abhydrolase_1 (363-498). E-value:
                     1.2e-08. Score: 26.8. Matches aSDomain:
                     nrpspksdomains_TU31_RS23455_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088741.1"
                     /locus_tag="TU31_RS23455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_01890"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_042158027.1"
                     /sec_met_domain="PF00561 (E-value: 5.2e-06, bitscore: 21.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 1.8e-36, bitscore:
                     121.2, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTTTAHLTALPSADTGFRVYHPALPEVCPRDPARMALAADADGR
                     CEIFTWNAVTGTARQVTDSPHGTLHCALDAEARVWWFAEDRHGHGLWYFQDFEGGARQP
                     GLTGLSPGRPHGLAVSDAGTVALGLGDGSGMTVHLGRPGGPARQVWTVDGPALLTGISP
                     SGGLLALSGAAGSDRAVTLVTCSGAVLDQLSGHHGRLWALGFAPSPAGTELLLVQEHDD
                     RYRLATWRPGSGLLPQDWCCFDTEITARWYPAGRRVLIRQDRHGRSTLAAADLDRRTLT
                     PVPSPPGTLLDAAPHPGDDVHYLWTDSATPPRMCSTAGTRLPELATLPTPAPGRHRELW
                     TPGPDGPVHTLLSEPEPVTGRRPPLVFLVHGGPADHDRDAYDPEVHSLVASGFAVARVN
                     YRGSTGYGPRWRAAYGAGVGLTQVADLVAVRGDLLRRGLARADAIGLWGTSWGGYLALL
                     ALGTRPDLWQAGVAVKPVADCAAAYRTGTPALRALDERLFGGTPDTVPERYARSSPLHY
                     AADVRAPLLVIAATLDTKCPPGQVRSYLGALRRAGAAHESMWLETGHDGYTGAHHVAVL
                     HRALSFLDQQLRRAPAAHRTPRPPVDGVSGGTGPSGAPGA"
     aSDomain        3979..4383
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TU31_RS23455_Abhydrolase_1.1"
                     /evalue="1.20E-08"
                     /label="TU31_RS23455_Abhydrolase_1.1"
                     /locus_tag="TU31_RS23455"
                     /protein_end="498"
                     /protein_start="363"
                     /score="26.8"
                     /tool="antismash"
                     /translation="PLVFLVHGGPADHDRDAYDPEVHSLVASGFAVARVNYRGSTGYGP
                     RWRAAYGAGVGLTQVADLVAVRGDLLRRGLARADAIGLWGTSWGGYLALLALGTRPDLW
                     QAGVAVKPVADCAAAYRTGTPALRALDERLF"
     gene            5001..6935
                     /locus_tag="TU31_RS23460"
                     /old_locus_tag="TPA0598_07_01910"
     CDS             5001..6935
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088743.1"
                     /locus_tag="TU31_RS23460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_01910"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_042158028.1"
                     /sec_met_domain="TIGR03975 (E-value: 6.7e-186, bitscore:
                     614.8, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILRRSVDERTSGRADVLHANLEFT
                     DWITRRTEFTADDYQYYALSSYFMGCGDWVFSSALYDDPEWRVPEFTASMRGKLRDERL
                     QMSKELHRVVPQFVQEIAERIVAAGPDVVGFTSTFQQNTAALAAAKYVKRLAPHIRTVM
                     GGANCDAEQGHATHRNFPFVDFVVRGEGEAAFPQLLTALDEGGDLSAVPGLCHRDADGE
                     SVANPMSTRPLPPATILPPDYSGYFERLASSVARNWVEPKLVVEGARGCWWGEKHHCTF
                     CGLNGSFMQFRSKSPETFYEEIMDLARRHRVLDMYLVDNILDMGYLTTVLPRIIDSGYD
                     LRMHIEIKANMRRPQLRTLAQAGMIYVQPGIESLNSRVLDLMDKGVSGCQNVRMLRDGA
                     ETGLSVSWNYLHGFPGESATDYEPVIAQIPALEHLDPPVDLSARIAIERFSPYFNRPEL
                     GFTGLRPEEHYRFTYDLPESELLDMAYVFEAPERGITKPTVTALNDALAAWKKHHADSR
                     LTHADLGDRIVLVSRRRAFDWGALELTAPTEIAAFRLLDQPHAPAALTRKLAARLPEHT
                     VDEAAVHALLQHWVTLGLVFTDGGQYVHLAPAAVNEDLLRLDFMRHTHAAAARPERPDD
                     DARALVAHV"
     gene            6984..7658
                     /locus_tag="TU31_RS23465"
                     /old_locus_tag="TPA0598_07_01920"
     CDS             6984..7658
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088744.1"
                     /locus_tag="TU31_RS23465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_01920"
                     /product="DUF5825 family protein"
                     /protein_id="WP_078885919.1"
                     /transl_table=11
                     /translation="MISTAAPSSRALTLHAWRDYDKDACALPGMSLGALDLTAPPDDQA
                     ARLWELGARRVEFTGEIDLTAVDDPADAAQAVRSLCLIRDLTARAVMVQWQLRLPPEPD
                     DGWRDLSHLQPPHTLTGPADPAAALAEWRNGHYLCKCLWRQGPGFVQIRDRRWGELRRF
                     TAEEPEYQQAIAQLSYGAPLRDVPPAIAADFLAERLVFRTGPLLWWLPYRVNRWIQEAM
                     AV"
     gene            7782..9389
                     /locus_tag="TU31_RS40460"
                     /old_locus_tag="TPA0598_07_01930"
     CDS             7782..9389
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /locus_tag="TU31_RS40460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_01930"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_052719021.1"
                     /sec_met_domain="Pkinase (E-value: 1.4e-43, bitscore:
                     144.7, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVRFAPLDDDDPTRVAGYDLRARLGAGGMGRVYLAFSPGGRALA
                     VKVVRPDYAQDEEFRRRFRKEIDAAQRVQGLYTAPVVDADSEAKLPWLATAYVPGPTLH
                     QAVTEHGPLPLLTVFRLLAGVAEGLTAIHGCELIHRDLKPANILLAEDGPRVIDFGIAH
                     AADATTLTSTHIRVGTPAFMAPEQIRGRSATPATDVFALGNLAVFAATGRTAFGEGNPD
                     ALFYRILNEAPELDDCPPTLRAVVERCLARDPGERPAVSEIMEYARKQTTGQTLALAGS
                     WLPEAVERSLAGYDTKAYVPPKTKKAGQGDTAPRTAETEKKDTAGGKAGTKTGGGAGVK
                     PPAGGGGSSSGGGSSSGGALAVVGLLAAVAFFVIGPDKVIGAIGDELKESPSPSSTYST
                     PSYTSSPDVNAQATGLTGGSDTSTPEPTEEATPDGCARGNQVFRSNLDMYDKASAASLS
                     SLASSLDSAASDAGDSEVASALRDLADDARALSDAASRFDAALNRRDSSQAETYKDAFS
                     SARQEWEDDAETFKSVCS"
     misc_feature    7842..7844
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(9444..10502)
                     /locus_tag="TU31_RS23475"
                     /old_locus_tag="TPA0598_07_01940"
     CDS             complement(9444..10502)
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025563.4"
                     /locus_tag="TU31_RS23475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_01940"
                     /product="RICIN domain-containing protein"
                     /protein_id="WP_042158031.1"
                     /transl_table=11
                     /translation="MKPTGHSGLRRTLTVFIAVLAVALGLTTQTGTAQADDGSGLNGLT
                     FTSVNNGRNLDVQNGNTGDGVFIVTNSAPGYHQKWNAGLQADGSFTLVNDATGKCVGAG
                     YPLKQQSCSGSSGLRWYFQPVSGAGDTFMIRNAGDNKCVDVVLGAQYNDAWTQTYGCNG
                     SKAQQWRVPSSATGAVFKAAVSYASKRCQKDATTCSWTKGSQAPAEPLPKQCVSPVWYN
                     GTEAPVPWTFSLSTSSGWSSQLGVSFTSTLGTGMASPVQTSVSLTLSGQVTYDLRTDLG
                     NSLVISVPSHQYGWVALSELATKVTGEWTFDANGFPWKAQDTVTVPLRNDDQGRASIYL
                     AQTSPDFTNCNS"
     misc_feature    complement(10476..10478)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(10594..11589)
                     /locus_tag="TU31_RS23480"
                     /old_locus_tag="TPA0598_07_01950"
     CDS             complement(10594..11589)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573501.1"
                     /locus_tag="TU31_RS23480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TPA0598_07_01950"
                     /product="DMT family transporter"
                     /protein_id="WP_063770820.1"
                     /transl_table=11
                     /translation="MGTTAVDGDVGSVRRANGRALAAASVTVVLWASSFIAIRSCAADF
                     GPGALALGRLVVASVVLSALLLARRGGFPAREAWPGILASGVLWFGAYMVALNWGERHV
                     DAGTASLVVNVGPILMALLGGWLLKEGFPPKLMAGMAVSFAGAVVVGLSMSGGGTSSLV
                     GVLLCLFAALAYAVGVVLQKPTLKHATPMQVTTFGALAGAVACLPFAGQLVSQVSRAPV
                     SATLQMVYLGVFPTALAFTTWAYAVSRTTVGKMGATTYAVPVLVVFLSWLFLGEIPGWL
                     TLLGGLLCLSGVAVSRMRGKAQPRVPEDARGGGADARAEGPAESAQRTAG"
     misc_feature    complement(11694..11708)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     misc_feature    11836..11850
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.07"
                     /tool="antismash"
ORIGIN
        1 ccggaattcg tggaggcccg ttatctgttc acgtctgccg gtgggaagaa ggaccgcggc
       61 gccggactgc tgtccggtga gggcttcggc ggcggcgagt cgcccgacgt catcaccgtc
      121 cgcaccgagt acccgtatca gaccctccaa gcggttcccg agaagtccga cctcccgtcc
      181 ttctcgctct gggccgctta cgtaccggag gacgcggagc agcgcaccaa ccacgcctat
      241 ggcctgctca tgatcgcgaa gccgccgatc ccgggagccc tgcatctggc ctggccgctc
      301 atccggcgct tcaccgagcg ggtgttcgcc gaggaccgga tggcggtcga ggccgagcag
      361 cgggcctggg acgagcaggg cgaggactgg aaccaggagg tcttcccgct gatcctcgat
      421 gtccgtgacg tgctgcgcac caacggggtc ccgatccgtc ccgaatccgc tctctgcggc
      481 gccgccgcgc tgtgcgacgg ctcgccgcgg gccgacgccg catcggggac cggcgcgcac
      541 cggccgggct gaccggccgg gcgtgcggtg gccggtggcc gggctcgcgc agctgccgtc
      601 tgaccgcaat caccgcaggt caggcgccct ccgtcggtga cgttcggtgg gtgcgcgggt
      661 gcagccggtg gtgggccgac ggtgggcggg ggatcgtggc aaggtggtgg gtgaggtgcc
      721 tccctcgccc atcccccgat tccgatgtgg aggctcgcat ggacgacaag gagttcttcc
      781 gggcagtggc ggaccgcgcg aggctctcgc ggcaggaagg ggccgatctc acccgcgcca
      841 cgctcgaaac gctggccctg cgtctgagcg acggcgaagc ccgcgatctg gccctcgaac
      901 ttcccgagcc gctgcgtgtc tcgctgaagc gggagcggcg ggagatggag atcttcgggc
      961 cggacgagtc gatccgcagg gtgcgagcgc gcaccggcct gtccgcgtcg gaagccgacc
     1021 gcggtgtacg ggcggtcctc ggcacgctcc aagaggtcat ctcccgcaag gagttcgagc
     1081 acgccatgtc gcaactcggc aaggagtatg cgcagttggt ggaggctgcg cgctgacggc
     1141 cggctccaag gcccgcgcgt ggcgagtggg ccgcagtatc ggccggcccg ccaccggctg
     1201 aggggtgagg gcggacggcc ggccgttcgg cgtggctccc gagtgggcgc accggatcgg
     1261 tcccacctga tccgcctgcc ctgtcgttcc gcttgaaggt gttcctcgca cacaccttgt
     1321 ggcccgcgtc actgtctggc taagctccgg cacgattcgg acacatttcc tccgtaagtt
     1381 gacctattgg tgtggttgat cttcatggtc tgcccacatg tgtgaggtgg cttcgcatgg
     1441 aacggtcaac ggaatccccg gcaggcggcg caccttacga cttaccgcgc atctcctccc
     1501 ggcccgtgac ggcggacggc ccgacacgcg gccgcaccag cggccgtacg caccacccgg
     1561 cacggaccac gtacggcgct gcccaccgcg gcaccgcgcg accggccccg ggccgcccct
     1621 gatgaccggg accgcgccgg cggtggcacc ggccgcaccg ctgccccatg tccgccgcga
     1681 tatccggctg ttctggtggg cgggcacctg cgacgccctg ggcagccaga catccgggct
     1741 ggtcctgccg ctgctgctgc tgaccctcgg ccggtcaccc gccgaggtcg gggccctcgc
     1801 cggactctcc gcggtcgcga cgctgctgct cggcccgttc gccgccgtcc ccgccgaccg
     1861 cggggcgcgc aagcagctga tggtgggctc cgcactggtc tccgccgccg cgatggccgg
     1921 ggtcaccgtc gccgtcgcgg cgggcccggt cccgttggcc gtgctcatcg ccgccgtgct
     1981 cgtcgagcgg tgcgcgactt cctgctacga ggccgcggcg ctcggcaccg tcgccctggt
     2041 gtgcccgccc ggggagcacc ggcgcgtcct gtcccgatta caggcggggg agggaggcgc
     2101 cctcgtgctc gggcccgcac tgggcgggtt gctcttccac gccgagcgct ggctgccgtt
     2161 cctcgcggac gccctctcct acgccgtcgc ggcgggctgt atcggcgcca tgcgcgcgcc
     2221 cctcgttccg ccccgtgagc agaagtcacc ggccggagcg gtacgccgag ggctgcggtc
     2281 ccgggccgcc gaagcagcgg ccggactggc gctgatccgc cgcgaaccct tcctgcggct
     2341 cgcgctcctg tggatctcgg ccgtcaacgc cctgctcgtc gcgctgtatt acacgacggt
     2401 gttcgccctt cagcacgacg gccggggcgc ggcaccgctg ggcctggtcc tcgcgctcgc
     2461 cggggcggcc ggtctggccg gagcgctcgc cgcaccccgg atcgccggcc ggcgctccgc
     2521 gggccgggtg ctggtgacgg tctcctggct gatggtgccg cccgccgctg ccctcgcgat
     2581 ggcccgcgac gcctggcact tcggcctgct cttcggtctg ctgtgtctgc tgacgccggt
     2641 ggccaccgtg gcgctacagg cccggatcat ccaggtcgtc ccacccggac tccaggcccg
     2701 caccgggacc gtgctggcca ccgtgtcggg tgcgaccgcc gccgtggcgc ccgccctggc
     2761 cgggctgtcc gccgaccggg ccggggccgc cgtcaccctc atgggctgcg ccgcgctgct
     2821 ggccgtgctc gcgctccaca cgacctgcgt ggcaccggcc cgcacccgcc gcgccgattc
     2881 ggagcccgca tgagcacgac cacggcacac ctcaccgcac tgccgtcggc cgacaccggc
     2941 ttccgggtct accacccggc gctgcccgag gtctgtccac gtgacccggc gcggatggcg
     3001 ctggccgcgg acgccgacgg ccgctgcgag atcttcacct ggaacgccgt caccggcacc
     3061 gcgcgccagg tcaccgacag cccgcacggc accctgcact gcgccctcga cgccgaggcc
     3121 cgggtgtggt ggttcgcgga ggaccgccac ggccacggac tgtggtactt ccaggacttc
     3181 gagggcggag cgcggcagcc gggcctgacc ggcctgtcgc cgggccgccc gcacggcctc
     3241 gccgtcagcg acgccgggac cgtggccctc ggcctcgggg acggcagcgg aatgaccgtc
     3301 caccttggga ggccgggcgg tccggcccgg caggtgtgga ccgtcgacgg gccggccctg
     3361 ctgacgggca tctcgccctc gggcgggctg ctggccctgt ccggggcggc cggctccgac
     3421 cgggcggtca cgctcgtgac ctgctcaggg gcggtgctcg accaactctc cggacaccac
     3481 ggccggctct gggcgctcgg cttcgcaccg tccccggcag gcaccgaact gctgctggtc
     3541 caggagcacg acgaccgcta ccggctggcg acatggcggc ccggcagcgg actgctgccc
     3601 caggactggt gctgcttcga caccgagatc accgcgcgct ggtaccccgc gggacgccgc
     3661 gtcctcatcc gccaggaccg ccacggccgt tccaccctcg ccgcggccga cctcgaccgg
     3721 cgcaccctga ccccggtacc gtccccgccc ggcaccctgc tcgacgccgc cccgcacccg
     3781 ggcgacgatg tgcactacct ctggaccgac agcgccaccc cgccccggat gtgctccacc
     3841 gccggcaccc ggctcccgga gctcgccacc ctgccgaccc cagcgcccgg ccggcaccgt
     3901 gaactgtgga cgcccggccc ggacgggccc gtgcacaccc tgctcagcga accggaaccg
     3961 gtcaccggcc gtcggccccc gctggtcttc ctggtgcacg gcggccccgc cgaccacgac
     4021 cgtgacgcct acgacccgga ggtgcactcc ctggtcgctt ccgggttcgc cgtcgcccgg
     4081 gtcaactacc gcggctccac cggctacggc ccccgctggc gcgccgcgta cggcgcgggc
     4141 gtcgggctca cccaggtcgc cgacctcgtc gcggtccgcg gcgatctgct ccgccgcggc
     4201 ctggcccgcg cggacgcgat cggcctgtgg ggcacctcct ggggcggcta tctggccctg
     4261 ctcgcgctcg gcacccggcc ggacctctgg caggcgggcg tcgccgtcaa acccgtcgcc
     4321 gactgcgcgg cggcgtaccg caccggcacc cccgccctgc gggccctgga cgagcggctg
     4381 ttcggcggca cgccggacac cgtgcccgag cgctacgccc ggagctcacc cctccactac
     4441 gccgccgacg tccgtgcccc gctgctggtg atcgccgcca ccctcgacac caaatgcccg
     4501 cctggccagg tacggagcta cctcggcgca ctgcggcggg ccggggcggc acacgagtcg
     4561 atgtggctgg agaccggcca cgacggctac accggcgccc accacgtggc cgtactgcac
     4621 cgcgcgctga gcttcctgga ccagcagctg cgccgcgctc ccgcggccca ccgaaccccc
     4681 cgtcccccgg tggacggggt ctccggaggc accggcccgt ccggcgcccc cggagcgtaa
     4741 gaaccgttcc gcaaccggca gaagaggaga agcagccatg cagaaggaca tcatccacaa
     4801 cgacccgctc gcgggtgacg aggagaaccg cagaccgagc atcggcgtca cggtgaccgt
     4861 cccgttccgt aacgccgagg acaccgagga ggactgaccc tccccggccg gcccggtggc
     4921 acaccgggcc ggccggaccg ccgcacctca ccggccggcc gccggccacc tcccacccca
     4981 ccgtcgtacg aggagccgcc atgcgcgtcc tgctggtcaa catgccctgg tccccgatcg
     5041 acctgccgtc cctcgccctc ggcatcctga gacgaagcgt cgacgagcga acctccggcc
     5101 gggccgatgt cctgcacgcg aacctggagt tcaccgactg gatcacccgg cggaccgagt
     5161 tcaccgcgga cgactaccag tactacgcac tctcctccta cttcatggga tgcggcgact
     5221 gggtgttctc ctccgccctc tatgacgacc ccgagtggcg ggtgccggag ttcaccgctt
     5281 cgatgcgcgg caaactgcgt gacgagcggt tgcagatgtc caaggaactg caccgtgtgg
     5341 taccgcagtt cgtgcaggag atcgccgagc ggatcgtcgc ggccggcccc gacgtcgtcg
     5401 gcttcacctc caccttccag cagaacaccg ccgcgctcgc cgccgccaag tacgtcaaac
     5461 gcctcgcccc gcacatcaga accgtcatgg gcggcgccaa ctgcgacgcc gagcagggcc
     5521 acgccaccca ccgcaacttc ccgttcgtgg acttcgtggt gcgcggcgag ggcgaggccg
     5581 ccttccccca gctgctcacc gcgctcgacg agggcggcga cctgtccgcc gtccccggac
     5641 tgtgccaccg cgacgccgac ggcgaaagcg tcgcgaaccc gatgagcacc agaccgctgc
     5701 cgcccgcgac catcctgccg cccgactaca gcggctactt cgagcgactg gcgtcctccg
     5761 tcgcccgcaa ctgggtggag cccaaactcg tcgtcgaggg ggcccgcggc tgctggtggg
     5821 gggagaagca ccactgcacc ttctgcggac tcaacggctc cttcatgcag ttccgcagca
     5881 agagccccga gaccttctac gaagagatca tggacctggc ccgccggcac cgggtgctgg
     5941 acatgtacct cgtcgacaac atcctcgaca tgggctacct caccaccgtc ctgccccgca
     6001 tcatcgacag cggctacgac ctgcggatgc acatcgagat caaggccaat atgcgccggc
     6061 cccagctgcg caccctcgcc caggccggaa tgatctacgt ccagccgggc atcgagagcc
     6121 tcaacagccg ggtgctggac ctgatggaca agggcgtcag cggctgccag aacgtccgca
     6181 tgctccggga cggagccgag accgggctct ccgtctcctg gaactacctc cacggcttcc
     6241 ccggggagag cgccacggac tacgagcccg tcatcgccca gataccggcc ctggaacacc
     6301 tcgacccgcc ggtcgacctc tccgcccgta tcgccatcga acgcttcagc ccgtacttca
     6361 accgtcccga actcggcttc accggtctgc gccccgagga gcactaccgg ttcacctacg
     6421 acctgcccga atccgaactc ctcgatatgg cctacgtctt cgaggcgccc gaacgcggca
     6481 tcaccaagcc caccgtcacc gcgctcaacg acgccctcgc cgcctggaag aagcaccacg
     6541 cggacagcag actcacccat gcggacctcg gcgaccgcat cgtgctcgtc agccgccggc
     6601 gcgccttcga ctggggcgcc ctggaactca ccgcacccac cgagatcgcc gccttccggc
     6661 tgctcgacca gccgcacgcc cccgccgccc tgacccgcaa gctcgccgcc cggctccccg
     6721 agcacaccgt cgacgaggcc gccgtgcacg ccctcctcca gcactgggtg acgctcgggc
     6781 tggtcttcac cgacggcggc cagtacgtcc acctggcacc tgccgccgtc aacgaggacc
     6841 tgctgcgcct cgacttcatg cgccacaccc acgccgcggc agcccggccg gaacggcccg
     6901 acgacgacgc ccgagccctc gtcgcccacg tctgatcccc gcccggcccg tagcaggact
     6961 cttatgcccc gcaggagaac gccatgatca gcaccgccgc cccatcctcc cgagcgctga
     7021 ccctccacgc ctggcgcgac tacgacaagg acgcctgcgc cctgccgggc atgagcctgg
     7081 gcgcgctcga cctgaccgcg ccgccggacg atcaggccgc ccggctgtgg gaactcgggg
     7141 cccgccgggt ggagttcacc ggggagatcg atctgaccgc ggtcgacgac cccgccgacg
     7201 ccgcccaggc cgtccggagc ctgtgcctga tccgcgatct gaccgcccgt gccgtcatgg
     7261 tgcagtggca gctgcggctg cccccggagc ccgacgacgg ctggcgcgac ctcagccacc
     7321 tccagccgcc ccataccctc accggccccg ccgaccccgc ggccgcgctg gccgagtggc
     7381 gcaacgggca ctacctctgc aagtgcctgt ggcgccaggg ccccggcttc gtccagatcc
     7441 gcgaccgccg ctggggcgaa ctgcgccgct tcaccgccga ggagcccgaa taccagcagg
     7501 cgatcgccca gctctcctac ggggcaccgc tccgcgacgt gcccccggcc atcgccgccg
     7561 acttcctggc ggagcgcctg gtcttccgca cggggcccct gctgtggtgg ctcccgtacc
     7621 gggtgaaccg gtggatccag gaggcgatgg ccgtctgagg accgcggggg agcgccggcg
     7681 catgcgcagc tccggtctgc gcatgcggca tgtatccgcc gtatgtctcg tgggatgctg
     7741 ccgaggtccg atgtcggtcc tgagacagca cgggggtacg gatggctgtc cggttcgctc
     7801 cgctcgacga cgacgatccg acccgggtgg ccggatacga cttacgcgca cggctcggcg
     7861 cgggcggcat ggggcgggtg tacctcgcct tcagccctgg tggccgggcg ctcgcggtga
     7921 aggtcgttcg gcccgactac gcccaggacg aggagttccg gcgccgcttc cggaaggaga
     7981 tcgacgccgc ccagcgcgtc cagggcctct acactgcacc ggtcgtcgac gccgacagcg
     8041 aggcgaagct gccctggctg gccaccgcct atgtgcccgg ccccaccttg caccaggccg
     8101 tcaccgagca cggtccgctg ccgctgctca cggtcttccg gctgctggcg ggcgtcgccg
     8161 agggcctcac cgcgatccac ggctgcgaac tgatccaccg cgacctcaaa ccggccaaca
     8221 tcctcctcgc cgaggacggc ccccgcgtca tcgacttcgg catcgcccac gccgccgacg
     8281 ccaccacgct caccagcacc cacatccgcg tcggcacacc cgcgttcatg gccccggaac
     8341 agatccgcgg gcgctccgcc accccggcca cggatgtctt cgcgctcggc aacctcgccg
     8401 tattcgccgc caccggacgt accgccttcg gcgagggcaa tcccgacgcc ctcttctacc
     8461 ggatcctcaa cgaggccccg gagctggacg attgtccgcc cacactgcgc gcggtcgtcg
     8521 agcggtgcct tgcccgggac cccggggagc ggcccgccgt cagcgagatc atggagtacg
     8581 ccaggaagca gacgaccggt cagacgctgg ccctcgccgg ttcctggctc cccgaggccg
     8641 tggagaggtc cctggccggg tacgacacca aggcgtatgt gccgcccaag accaagaagg
     8701 ccggccaggg cgacacggcg ccccgcaccg ccgagaccga gaagaaggac acggctggcg
     8761 gcaaggccgg taccaagacg gggggagggg ccggcgtcaa gccgccggcc ggtggcgggg
     8821 gaagctcgtc cggcggggga agctcgtccg gtggcgcgct cgccgtggtt ggactgctcg
     8881 ccgcggtggc gttcttcgtc atcggcccgg acaaggtcat cggcgcgatc ggcgacgagc
     8941 tcaaggaatc gccgtccccc tcatcgacgt actccacacc gtcgtacacc tcgtcgccgg
     9001 acgtgaacgc gcaggcgacc ggtctgaccg gcggcagcga cacctcgacc ccggagccga
     9061 cggaggaagc gacccccgac ggctgtgcgc ggggcaatca ggtcttccgc agcaacctcg
     9121 atatgtacga caaggcgagc gccgcctccc tgagctcgct ggcctccagc ctcgactcag
     9181 cggcgagcga cgcgggcgac tcggaggtgg cctcggcgct ccgcgacctc gccgacgacg
     9241 cgcgggcgct gagtgacgcg gcatcacggt tcgacgccgc gctcaaccgc cgcgacagct
     9301 cacaggccga gacctacaag gacgcgttca gctcggcgcg ccaggagtgg gaggacgacg
     9361 ccgagacctt caagtccgtg tgctcctaga ccgcgtccgc ttcagggtgg gcccgcacga
     9421 tccgtgcggg cccgccttct ggttcagctg ttgcagttcg tgaagtccgg gctggtctgc
     9481 gccagataga tgctggcacg gccctggtca tcgttgcgca gcggaaccgt gaccgtgtcc
     9541 tgtgccttcc acgggaagcc gttcgcgtcg aaggtccact caccggtcac cttcgtcgcc
     9601 agctcggaca gcgccaccca gccgtactga tgcgacggca cggagatcac caggctgttg
     9661 ccgagatccg tccgcagatc gtaggtgacc tggccggaga gggtgagact gacgctggtc
     9721 tgcaccgggc tcgccatgcc cgtgccgagg gtggaggtga aggacactcc gagctggctc
     9781 gaccagccgc tggaggtgct cagcgagaac gtccacggca ccggcgcctc ggtgccgttg
     9841 taccagacgg gagagacgca ctgcttggga aggggctcgg ccggggcctg cgaacccttc
     9901 gtccacgagc aggttgtggc gtccttctgg cagcgcttcg acgcatagga caccgcggcc
     9961 ttgaacacgg cgccggtcgc cgacgagggg accctccact gctgcgcctt gctgccgttg
    10021 cagccgtagg tctgggtcca tgcgtcgttg tactgggcgc ccagcacgac gtcgacgcac
    10081 ttgttgtcac cggcgttacg gatcatgaaa gtgtcgccgg cgccgctcac gggctggaag
    10141 taccagcgca gaccggacga tccggaacag gactgctgct tgagcggata gcccgctccg
    10201 acgcacttgc ccgtggcgtc gttgacgagg gtgaacgatc catcggcctg aagaccggcg
    10261 ttccacttct ggtggtagcc cggcgcggag ttggtgacga tgaagacacc gtccccggtg
    10321 ttgccgttct gcacatccag gttccggccg ttgttgaccg atgtgaacgt caggccgttc
    10381 aggcccgagc cgtcatcggc ctgggccgta ccggtctgtg tggtcaggcc gagggcgacg
    10441 gcgagcacgg cgatgaagac ggtgagtgtt ctgcgtaaac cgctgtgtcc ggtcggtttc
    10501 ataccagaac aaccccctta tctgatcgag gtgttggtgg tgtggtccac cgtgttccgc
    10561 acgctatcgc ccccgatgcc cgggcggcag tgctcagccg gcagtgcgct gcgcggactc
    10621 cgccggcccc tccgcccgcg cgtccgcacc gccccctcgt gcgtcctccg ggacccgcgg
    10681 ctgcgccttc ccccgcatcc gcgacaccgc caccccggac aggcagagga gaccaccgag
    10741 gagcgtcagc cagccgggta tctcgccgag gaacagccag gacaggaaga cgacgagaac
    10801 cggtaccgcg tacgtcgtgg cgcccatctt cccgacggtc gtacgggaca ccgcataggc
    10861 ccacgtggtg aacgcaaggg cggtcgggaa cacacccagg tacaccatct gcaaggtcgc
    10921 cgataccggg gcccggctca cctgagacac caactgcccg gcgaacggca gacaggccac
    10981 cgctccggcg agcgcgccga acgtcgtcac ctgcatcggt gtggcgtgct tcagggtcgg
    11041 cttctgaagc accacaccca ccgcgtatgc cagcgccgcg aacagacaca gcaggacgcc
    11101 gacgagagac gacgtgccac cgccggacat ggacagtccg accaccacgg ccccggcgaa
    11161 cgacaccgcc atgcccgcca tcagcttcgg cgggaagccc tccttcagca gccaccctcc
    11221 cagcagcgcc atcaggatcg gcccgacgtt gacgaccagg gaggccgtgc cggcatccac
    11281 atgccgctca ccccagttca acgcgaccat gtacgcgccg aaccacagga ctccggaggc
    11341 gaggatgccc ggccaggcct cgcgggccgg gaatccacca cgccgggcca gcaggagggc
    11401 gctcaacacg acggacgcca ccaccagccg gcccagtgcg agcgcgcccg gcccgaaatc
    11461 cgcggcgcaa ctacggatcg cgatgaacga cgacgcccac agcaccacgg tgacggaggc
    11521 cgcggcgagc gctcgcccgt tcgcccgccg cactgacccg acatcgccgt ccaccgctgt
    11581 tgttcccacc cgccggctca ctccggtgtc ctcccaaagc tctccacgta cgccctgcgc
    11641 gcacatgagc cgacagcttg taagaggccg caccgcactg acaagcgaat accggggccg
    11701 gtcgggccat gccgggaatc cggcagtccc ggtgccggcg cgttcgccgt tctgccgagg
    11761 cggcaggacg tcaagcccgg ccgccgccga gggcagcaga cgaccacccc gctccacggc
    11821 gagtgtggtc accggtgagg agctcggcca accgccgggt catttccgtg gggttggcgg
    11881 cgtggacgag ttcggtgcgg tgtatcagcc ggggctctgc caccggcacg gcggt
//
