LOCUS       NZ_CDME01000011        10396 bp    DNA     linear   CON 13-AUG-2024
DEFINITION  Kibdelosporangium sp. MJ126-NF4, whole genome shotgun sequence.
ACCESSION   NZ_CDME01000011
VERSION     NZ_CDME01000011.1
KEYWORDS    WGS; RefSeq.
SOURCE      Kibdelosporangium sp. MJ126-NF4
  ORGANISM  Kibdelosporangium sp. MJ126-NF4
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Kibdelosporangium.
REFERENCE   1
  AUTHORS   Ramaraj,Thiru.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2014) National Center for Genome Resources (NCGR),
            National Center for Genome Resources (NCGR), 2935 Rodeo Park Drive
            East, Santa Fe, New Mexico, 87505, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000826545.1-RS_2024_08_13
            Annotation Date                   :: 08/13/2024 03:10:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,648
            CDSs (total)                      :: 10,573
            Genes (coding)                    :: 10,470
            CDSs (with protein)               :: 10,470
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 19 of 103
            Pseudo Genes (incomplete)         :: 89 of 103
            Pseudo Genes (internal stop)      :: 5 of 103
            Pseudo Genes (multiple problems)  :: 10 of 103
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:05:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1013609
            Orig. end                         :: 1024005
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CDME01000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10396
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10396
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10396
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5396](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5396)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(197..212)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     gene            302..1087
                     /locus_tag="BN1199_RS33180"
     CDS             302..1087
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459638.1"
                     /locus_tag="BN1199_RS33180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_042190868.1"
                     /transl_table=11
                     /translation="MGWGSWVAVGDSFTEGLNDVDDDGGFRGWADRLAELMGDRDPNFR
                     YANLAIRGKMMDEIIDEQVPLAIEANPDLVTFCAGGNDLIVPGSDADELAARLEQAIVD
                     LQAAGSSVLIFAGPDPKAMPIMRRVRAKVAIFNEHLHAIADRHGAMIVDLWAMHVLGDE
                     RAWSDDRLHFSAEGHRRIALRTAEVLGIPTEEDWREPWPPQERDTSWLKMRKADLDWTR
                     VHLVPWVKRQLRGESMGDGVQPKRPELRPLGDMSDCQKS"
     gene            complement(1183..3819)
                     /locus_tag="BN1199_RS33185"
     CDS             complement(1183..3819)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /locus_tag="BN1199_RS33185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_042190870.1"
                     /transl_table=11
                     /translation="MSTGENDEEDHAVVRFGVLGPVQAVVNGEPVALGGPGVRGLLAML
                     LLRPNEVVPVEDILDGLWGEDPPLTARTIVQNYVSRLRRQLRLVDPTGSVWIDTQLPGY
                     QLIVDEDLIDATRATGLLTRARHEHPVRRAEMLREALGLWRGPVLADVSPRISAPELDD
                     LRFAVLEARVEADLDLGRHAELIGELATFAEAYPFREQMIAHLLLALYRTGRRADALEA
                     YQRFARRAADDLGIDPGPALRELHERVLNDDSTLLLPAPAPLVPPKVGVLVPGQLPPAP
                     VGFHGRDEELVWLDQLLAEDHGEATPIGVLGGPAGIGKTALATMWGRMVGHEFPDGQLF
                     AGLRGFHSEHTPLEPGDVLTQFLLTLGVPATEIPADTDDRAALYRSLLAHRRVLVLLDD
                     ARDSAQVRLLLPGGSRALVLITSRVRLEAMVARSGARLLDLQPLPAADSVRVIDSITGH
                     GRMDRSRLEQLASLSGGLPLALRIVGARLAARQDWIADELIAELGDESTRLTALAAEHS
                     DTSVRAAFDLTYRRLAEPDAFRLLGLVPGPSVSPAATAVLQSVDTATARRSLRELADAY
                     LVSETSRDVFVMHDLVRLHAKELAPVGEERAAAVDRLVTYYLNAANTARHFLGPVAFPL
                     EKTDNSGIGSAFHAVEWFEREWANLVAVLDVAAPGDVWRLARMAHDFRVVHPVWQPWQA
                     IVHKGLSSAVSAGDLEGEAWSRHSMCAMYAQFGRAGETLDEATRIGEIAAELGDSRLAS
                     VGEEALAAAHYGLGNHREAVVAYEKAIERYRDSGDDELSVFAVGRLALLHGEMGDEQAA
                     ERHARAAVALSAAVFAEAVSREILGAVLSAQGAQSAARQQRERSAELYAQVKSAKAEEM
                     RDLTDDV"
     misc_feature    complement(2038..2040)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(3517..3519)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(3679..3681)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    3881..3892
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.27"
                     /tool="antismash"
     gene            complement(4093..5004)
                     /gene="thpD"
                     /locus_tag="BN1199_RS33190"
     CDS             complement(4093..5004)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="BN1199_RS33190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_042190872.1"
                     /sec_met_domain="PhyH (E-value: 2.4e-31, bitscore: 106.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTGIRPVDRYPTRVIGEPGLIDRTDPTVWADTPGPVDAATLAS
                     HEAKGYSIDDAVLSKQEVQTYWQELVRLTGDPELHSDERVVIEKESQEIRSIFEVHRIS
                     QLIAELIRDPRILDRARQLLGSEVYVHQSRVNFMPGFKGKGFYWHSDFETWHAEDGMPA
                     PRAVSMSLALTDNYPFNGGLMVMPGSHRTFVPCTGETPANHYRESLKEQEIGVPSRENI
                     TQLAAKHGIDQFTGEAGSALWFDANIMHGSGNNITPFPRSNIFVVFNSVENALQEPYAA
                     PAPRPTFIAARDFTPLTRSTRV"
     gene            complement(5001..5396)
                     /locus_tag="BN1199_RS33195"
     CDS             complement(5001..5396)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787751.1"
                     /locus_tag="BN1199_RS33195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_042190875.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.4e-55, bitscore:
                     182.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTTNDVTDTDRDVQTPNWRSKRIILAGEQVGFSVHETTLYAG
                     TVNDFWYANHVEAVFVVEGEGELEDKETGTVYQLTPGSLYLLNGHERHQLRPRTQMRTV
                     CVFNPPVTGREVHDENGVYPLIVEGAT"
     gene            complement(5393..6646)
                     /gene="ectB"
                     /locus_tag="BN1199_RS33200"
     CDS             complement(5393..6646)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-357). E-value: 1.3e-71.
                     Score: 233.3. Matches aSDomain:
                     nrpspksdomains_BN1199_RS33200_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631928.1"
                     /locus_tag="BN1199_RS33200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_042190877.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.3e-75, bitscore:
                     248.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIFETLESEVRSYCRGWPTTFDKATGSVLHDESGKEYLDFFAGA
                     GALNYGHNNPSLKRAVLDYMLADGVIHSLDMNTVAKRDLLESFQDIVLKPRELDYKVMF
                     PGPAGANAVEAALKLARKVTGRESIINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHA
                     TPMPYDNYFDGQTPDFLFFERLLEDSGSGLNEPAAVIVETVQGEGGINAARVEWLRGLS
                     DLCKRHSILLIVDDVQMGCGRTGPFFSFEAAGIEPDIVCLSKSIGGYGLPLALTLFKPE
                     LDVWEPGEHNGTFRGINPAFVAATEALKVYWQDDKLEKSTLAKGEQVGRALTGLVESYS
                     DKAGMRAKGRGLARGLQFDDAALADKVCSVAFERGLLMETSGPDGEVVKLMPPLTITDD
                     ELEHGLGIVAQSVKGALA"
     aSDomain        complement(5576..6580)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BN1199_RS33200_Aminotran_3.1"
                     /evalue="1.30E-71"
                     /label="BN1199_RS33200_Aminotran_3.1"
                     /locus_tag="BN1199_RS33200"
                     /protein_end="357"
                     /protein_start="22"
                     /score="233.3"
                     /tool="antismash"
                     /translation="DKATGSVLHDESGKEYLDFFAGAGALNYGHNNPSLKRAVLDYMLA
                     DGVIHSLDMNTVAKRDLLESFQDIVLKPRELDYKVMFPGPAGANAVEAALKLARKVTGR
                     ESIINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHATPMPYDNYFDGQTPDFLFFERL
                     LEDSGSGLNEPAAVIVETVQGEGGINAARVEWLRGLSDLCKRHSILLIVDDVQMGCGRT
                     GPFFSFEAAGIEPDIVCLSKSIGGYGLPLALTLFKPELDVWEPGEHNGTFRGINPAFVA
                     ATEALKVYWQDDKLEKSTLAKGEQVGRALTGLVESYSDKAGMRAKGRGLARGLQ"
     gene            complement(6698..7213)
                     /gene="ectA"
                     /locus_tag="BN1199_RS33205"
     CDS             complement(6698..7213)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466527.1"
                     /locus_tag="BN1199_RS33205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_042190879.1"
                     /transl_table=11
                     /translation="MSGKTFGQAVVDVPSTSDGAGMWRLARDSRSLDLNSSYSYLLWCR
                     DFAGTSAVMRVDDDVIGFVTGYLRPNQADTLVIWQIAVADEHRGRGFAATMLDALVRRT
                     GARHLEATVTSDNDASIAMFRALATRWGAGLTGNQLFSADQFPDDHAPEFLFRIGPFLS
                     VSAPSPLS"
     gene            complement(7377..8405)
                     /gene="mgrA"
                     /locus_tag="BN1199_RS33210"
     CDS             complement(7377..8405)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="mgrA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155093.1"
                     /locus_tag="BN1199_RS33210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="WP_042190880.1"
                     /transl_table=11
                     /translation="MVYRADERRYDNAEFRRSGRSGLKLPLISLGLWQNFGGSRPYEHG
                     RQIARRAFDLGVTHFDLANNYGPPYGSAEENFGRILRDDLRPYRDELLISTKAGYDMWP
                     GPYGDFGSRKYLLASLDQSLGRMGLDYVDIFYSHRFDPDTPLEETVGALVSAVQQGKAL
                     YVGISSYSPAKTKEAADLLRQAGVPLLIHQPSYSMLNRWIEPELLDTLAEVGAGCIAFS
                     PLGQGLLTDRYLNGVPEGSRATQQGSLHQEHLSEENLDRVRGLNGIAQRRGQKLSQLAL
                     AWVLRDPRVTSALIGVSSVEQLEENLAALDSAPLTADELAEVDKYAVESGLDLWRPSRV
                     AG"
     gene            complement(8632..9366)
                     /locus_tag="BN1199_RS33215"
     CDS             complement(8632..9366)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BN1199_RS33215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_052478638.1"
                     /transl_table=11
                     /translation="MPDTGFRRSLYVGVDIRGYGAAPEQRQQKWQEILLRSLDRAASSV
                     GLNRAHWTRQQQGDAELAVVPHHEPEPVIVDGFTRRLEAELRQHNADVLPCARLRLRMA
                     VHHGVVVNGPNGFPGRDAVVVGRLLDSKPLRGVLAQWPEANLAVMLSELVYRSVVEAGH
                     TSLRTDQFRRLAVREKEFKGYGWLWLPGCSDSIDMYARPIVPRWDSADVRARPYVVRAE
                     EVSEVVVRGRHRATAQRGYIGG"
     misc_feature    complement(9355..9373)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 19.01"
                     /tool="antismash"
     misc_feature    complement(9409..9427)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 21.52"
                     /tool="antismash"
     misc_feature    9411..9424
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.92"
                     /tool="antismash"
     misc_feature    9499..9508
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.77"
                     /tool="antismash"
     misc_feature    9501..9514
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.68"
                     /tool="antismash"
     gene            complement(9521..10231)
                     /locus_tag="BN1199_RS33220"
     CDS             complement(9521..10231)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /locus_tag="BN1199_RS33220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_198151802.1"
                     /transl_table=11
                     /translation="MGKIIPRRVAGGALPYPQTSLLGRLSERTREDFLAVGVSESRPAG
                     VTIINQGENDSSALLLLHGSVKVLATDPAGERTLLAIRVGGDLVGEMAALDVGPRSATV
                     TACTDIFYKRMTKAELRNFLARRPDAAIEVATMLSERLRWADRRRIEHGRPASVRVARV
                     LVDLYEAYGTRSSGHWDLTVPLTRDELASIAGIKLSTAEKIIRAFQQEGLVSGRYRGIA
                     VLDMARLRVRAEIT"
ORIGIN
        1 tgggcgttcc agtcgtccat cttctcgctc gcgtgctgcg agcgtgggtc gactggttgc
       61 tgcgatcctt ccggcatcgt gtcccccatt taggcgtacc ctctccaacc ccgcatggta
      121 actgtgatgt gagaatcgcg tgatcggttc ccgcatgcac acggagtcgt gatgggacag
      181 tgataataca ctgtgtgtga cattgatgtc acttctcgca accgacggtg gcgtaggtag
      241 gtgtccatta ggcatcctga tggtttagtt gtgcgtgaca acggccttcg ggagggatgg
      301 cgtgggatgg ggcagctggg tcgcggtggg ggacagcttc accgaggggc tgaacgacgt
      361 tgatgatgat gggggctttc ggggctgggc ggatcgcctt gccgagctga tgggtgaccg
      421 ggatccgaac ttccggtacg ccaatctcgc gattcgcggg aagatgatgg acgagatcat
      481 cgacgagcag gtgccgctgg ccatcgaggc caacccggac ctggtcacgt tctgtgccgg
      541 tggcaatgac ctgatcgtgc ccggcagtga cgcggacgag ctcgccgccc ggcttgagca
      601 ggccattgtg gacctgcagg ccgccggcag ctcggtgctg atcttcgccg gtcccgaccc
      661 caaggcgatg ccgatcatgc ggcgggttcg ggcgaaggtg gccattttca acgagcacct
      721 gcacgcgatc gcggaccggc acggggcgat gatcgtcgac ctgtgggcca tgcacgtcct
      781 cggcgacgag cgggcgtgga gtgacgaccg tctgcacttc tccgcggagg ggcaccgacg
      841 gatagcgttg cgcaccgccg aggtgctcgg tatacccacc gaggaggact ggcgcgagcc
      901 gtggccgccg caggagcggg acacctcctg gttgaagatg cgcaaagccg atctcgactg
      961 gacgcgggtg cacctggtgc cgtgggtcaa gcgccagctg cgtggcgagt ccatggggga
     1021 cggtgtgcag cccaagcgtc ctgagctgcg cccgctcggt gacatgtcgg attgtcagaa
     1081 gagttgagca gcccggccgg cactccccct cggcgtgccg gccgcccccg gtggcgcccg
     1141 gcgtagcctg ccagttcggc gaacaacatc actaaccgtg cgctaaacgt cgtcggtcag
     1201 atcccgcatt tcttccgcct tggccgattt gacctgcgcg tacagctcag cagatcgctc
     1261 ccgttgttgg cgcgcagccg attgcgcgcc ctgcgcggac agcaccgcgc cgaggatctc
     1321 gcggctgaca gcctcggcga acaccgcggc ggacagtgcg accgcggcgc gggcatgtcg
     1381 ttcagcggcc tgttcgtcgc ccatttcccc gtgcagcaag gcgagcctgc cgacggcgaa
     1441 caccgagagc tcgtcgtcac cggaatcccg gtagcgctcg atggctttct cgtacgcgac
     1501 aacggcttcg cggtggttgc cgagtccata gtgggcagcc gcgagcgcct cctcgcccac
     1561 cgaggccagt cgggagtcgc ccagttccgc cgcgatctcc ccgatccgcg tggcctcgtc
     1621 gagggtctct ccggcacggc cgaactgcgc gtacatggcg cacatgctgt ggcgtgacca
     1681 ggcctcaccc tcaagatcac cagcggagac ggcgctgctt agtcctttat ggacgatcgc
     1741 ctgccacggc tgccacacgg ggtgtaccac ccggaagtcg tgggccatgc gggcgagccg
     1801 ccacacgtca cccggcgccg cgacatccag cacagcaacg aggttggccc actcccgctc
     1861 gaaccactcc accgcgtgga aagccgatcc gatacccgag ttgtctgttt tctccagggg
     1921 aaacgccacc ggccccagga agtgccgtgc cgtgttggcg gcgttgaggt agtacgtgac
     1981 gagccggtcg accgcggccg cccgctcctc gccgacgggc gcgagttcct tggcgtgtaa
     2041 tcgaacgaga tcgtgcatga cgaacacgtc ccggctggtc tcggacacga ggtacgcgtc
     2101 ggcgagctcg cgcaggctcc ggcgggccgt cgcggtgtcg accgattgca gcacagcggt
     2161 ggcggccggg ctgaccgacg gaccggggac cagcccgagc agccggaagg cgtccggctc
     2221 ggcgagacgg cgatacgtca ggtcgaaagc ggcgcgcacg ctcgtgtcgg agtgctccgc
     2281 cgccagcgcg gtcagcctgg tggactcatc gccgagttcg gcgatcagct cgtcggcgat
     2341 ccagtcctgg cgcgcggcca ggcgggcacc gacgatccgc agcgccagcg gcaaaccacc
     2401 ggacaacgac gccagttgct caagtctgga cctgtccatc cgcccgtgac cggtgatgga
     2461 gtcgatcacg cgcaccgagt cggccgccgg aagtggttgc aggtcaagca gtcgcgcccc
     2521 actgcgggcg accatcgcct cgagccggac ccggctggtg atcagcacga gggcgcgcga
     2581 accacccggc agcaacagcc ggacctgcgc ggaatcccgt gcgtcgtcga gcagtacgag
     2641 cacccgccgg tgcgccagca gcgaacggta gagcgcggcg cggtcgtcgg tgtccgccgg
     2701 gatctcggtg gcaggcacgc cgagcgtgag caggaactgg gtgagcacgt cgcccggctc
     2761 cagcggtgtg tgctccgagt ggaacccgcg cagcccggcg aacagctggc cgtcggggaa
     2821 ctcgtggccg accatccggc cccacattgt cgccaacgcg gtcttgccga tccctgcggg
     2881 cccaccgagc acaccgatcg gtgtggcctc accgtggtcc tcggcgagca actggtcgag
     2941 ccacaccaac tcctcgtcgc ggccgtggaa gccgaccggc gcgggcggca gctggccggg
     3001 cacgagcaca ccgaccttcg gcggcacgag cggagcgggc gcgggcagca gcaacgtgga
     3061 gtcgtcgttg agcacgcgct cgtgcagttc ccgcaaggcc gggcctggat cgataccgag
     3121 gtcgtccgcg gcgcgccggg caaaccgttg gtacgcctca agagcgtccg cgcgcctgcc
     3181 ggtccggtac aacgcgagca gcaggtgggc gatcatctgt tcccggaacg ggtacgcctc
     3241 ggcgaacgtc gccagctcgc cgatcagctc ggcgtgcctg cccaggtcga ggtcggcctc
     3301 gacccgtgcc tccagcacgg cgaaccgcag atcgtccagt tccggtgcgc tgatccgggg
     3361 cgagacgtcg gcgagcaccg ggccacgcca caacccgagc gcctcgcgca gcatttccgc
     3421 ccggcggacc ggatgttcgt gtctggcgcg ggtgagcagc ccggtggcgc gagtggcgtc
     3481 gatcaggtcc tcgtcgacga tcaactggta gccgggtaac tgtgtgtcga tccacaccga
     3541 cccggtcggg tcgaccaggc gcagctgcct gcgcagccgg gaaacgtagt tctgcactat
     3601 ggtccgcgcg gtcagtggcg ggtcctcgcc ccacagcccg tcgaggatgt cctctaccgg
     3661 aacgacctcg ttgggacgta acagaagcat cgccaacagg ccacgcacgc ccggcccgcc
     3721 gagcgcgacc ggttccccgt tgaccaccgc ctgcaccggt cccagcactc cgaaccgaac
     3781 gacggcatgg tcctcctcgt cattctcccc cgtactcaca acaaccctcc cccgggtcct
     3841 tactaccggt gaccaaggta agtcgacacc ggggggttaa gtaaaggttt actcatcatt
     3901 cggccagaag gcccagtcac aagtcggccc cttgccgacc ggtcccgatc cggttggcaa
     3961 ggggccacac tcacacggcc tgatcacggg tttcgccgac cccaccccga gtgggggacc
     4021 gactcacccg cgaccgtcat ccggctgagc gagatccatg cgtctcaaat gtaacagagc
     4081 gcatgtgatc catcacaccc gggtcgaccg cgtcaaaggg gtgaaatcgc gggccgcgat
     4141 gaaggtcggc cgcggcgcgg gcgccgcgta cggctcctgc aaggcgttct caacgctgtt
     4201 gaacacaacg aagatgttcg accgcgggaa cggcgtgatg ttgttgccgg agccgtgcat
     4261 gatgttcgcg tcgaaccaca gcgcggaccc ggcttcgccg gtgaactggt cgatgccgtg
     4321 cttggcggcg agctgggtga tgttctcccg gctcggcacg ccgatctcct gctccttcaa
     4381 cgactcccgg tagtggttcg ccggtgtctc gccggtgcac ggcacgaatg tccggtgcga
     4441 gcccggcatg accatcagtc cgccgttgaa ggggtagttg tcggtcagcg cgagcgacat
     4501 gctgaccgcg cgcggcgcgg gcatgccgtc ctcggcgtgc cacgtctcga agtccgagtg
     4561 ccagtagaag cccttgccct tgaagccagg catgaagttc acccggctct ggtgcacgta
     4621 gacctcggag ccgagcagtt gccgcgcccg gtcgaggatc ctcgggtccc tgatcagctc
     4681 ggcgatcagc tggctgatcc ggtgcacctc gaagatcgac cggatctcct gcgactcctt
     4741 ctcgatcacc acgcgctcgt ccgagtgcag ctcggggtcg ccggtcagtc gtaccagttc
     4801 ctgccagtac gtctggacct cctgcttgct cagcacggcg tcgtcgatcg agtacccctt
     4861 ggcctcgtgc gacgccaggg tggcggcgtc caccgggccg ggcgtgtccg cccacaccgt
     4921 ggggtcggtt cggtcgatca gaccaggttc gccgatgacc cgggtcgggt agcggtcaac
     4981 gggccggatg ccggtcagag tcatgtggct ccttccacga tcagggggta gacgccgttt
     5041 tcgtcatgga cttcgcggcc ggtcacgggt gggttgaaca cgcagaccgt gcgcatctgg
     5101 gtacgcggcc gcaactggtg gcgctcgtgg ccgttgagca ggtagagcga gcccggcgtg
     5161 agttggtaca ccgtgccggt ttccttgtcc tccagttcgc cctcgccctc gacgacgaag
     5221 acggcctcga cgtggttggc gtaccagaag tcgttgactg ttcccgcgta cagcgttgtc
     5281 tcgtgcacgg agaagccgac ctgctcaccg gcgaggatga ttctcttgct gcgccagttc
     5341 ggcgtctgca cgtcccgatc ggtgtcggtg acgtcgttgg tcgtgcggac gatcaagcca
     5401 gtgctccctt cacggactgt gcgacgatgc ccagtccatg ctccagttcg tcgtcggtga
     5461 tagtcaacgg cggcatcagc ttcacgacct caccgtcggg gccggacgtc tccatcagca
     5521 ggccgcgctc gaaggcgacg ctgcacacct tgtcggcgag cgcggcgtcg tcgaactgca
     5581 gacctcgtgc caggccacgg cctttcgcac gcatccctgc cttgtccgaa taggactcga
     5641 cgagtccggt cagcgcccgg ccgacctgct cgcccttggc cagggtgctc ttctccagct
     5701 tgtcgtcctg ccagtacacc ttcagcgcct cggtcgcggc gacgaaagcc gggttgatgc
     5761 cgcggaaggt cccgttgtgc tcgccgggct cccacacgtc cagctccggc ttgaacaagg
     5821 tcagcgcgag cggaagtccg tacccgccaa tggacttgga caggcagacg atgtcgggct
     5881 cgatccccgc ggcctcgaag ctgaagaacg gcccggtccg gccgcagccc atctggacgt
     5941 cgtcgacgat cagcaggatc gagtgccgct tgcagagatc cgacagcccg cgcagccatt
     6001 cgacccgagc ggcgttgatg ccgccctcgc cctggacggt ctcgacgatc acggccgccg
     6061 gttcgttcag tccgctgccg ctgtcttcga gaagcctttc gaagaacagg aaatccgggg
     6121 tctggccgtc gaagtagttg tcgtacggca tgggcgtggc gtgcacgagc gggattcccg
     6181 caccaccgcg cttcatcgag ttgccggtga ccgacaacgc gccgagcgtc atgccgtgga
     6241 acgcgttggt gaagttgatg atcgactcgc ggccggtcac cttgcgggcc agcttcagcg
     6301 cggcctcgac cgcgttcgcg ccggccggtc cggggaacat caccttgtag tcgagctcgc
     6361 gcggcttgag cacgatgtcc tgaaaggact cgagcaggtc acgctttgcc acggtgttca
     6421 tgtcgagcga gtggatgacg ccgtcggcga gcatgtagtc gagcacggcc cgtttcagcg
     6481 acgggttgtt gtgcccgtag ttgagagcgc cggcaccggc gaagaagtcc aggtactcct
     6541 tgccggactc gtcgtgcagc acgctgccgg tggccttgtc gaaggtggtc ggccagccac
     6601 ggcagtagct gcggacctcg gattcgagtg tctcgaaaat ggtcacggtt ctccgcgttg
     6661 tgttcgaggg agcagtgcgc aggtgatctg ggcaggctca ggagagtggg gacggcgcgc
     6721 tgacggacag gaacgggccg atccggaaga ggaactcggg cgcgtggtcg tcagggaact
     6781 ggtcggcgct gaacagctgg ttgccggtca gccccgcacc ccagcgtgtg gcgagtgccc
     6841 ggaacatcgc gatggacgcg tcgttgtccg aggtgacagt cgcttcgaga tgtctcgcgc
     6901 cggtccggcg gaccagggcg tcgagcatgg tggcggcgaa acctctgccg cggtgctcgt
     6961 cggcgacagc gatctgccag atcaccagcg tgtcagcctg gttggggcgc agatacccgg
     7021 tgacaaagcc gatcacgtcg tcatcgacgc gcatgaccgc ggaggtaccg gcgaaatctc
     7081 tgcaccacag caggtaggag tacgaagagt tcaggtcgag cgagcgcgaa tcccgcgcca
     7141 gccgccacat acccgcgccg tccgatgtgg acggcacatc cactactgcc tggccgaagg
     7201 tttttccgga catgccaagg gaacgtaaca gaggtgtttc gcccggtcag ctcaccggaa
     7261 cgtggatccg gccgtgcacc tgggggaatg cgaaacgcac tgtggtgatc agtacagagt
     7321 gttgatcagc gcgtgcgcgc ccgaatagcg tcgcgcgcac gcgcgttccc agaacgtcag
     7381 cccgctacac gtgacggccg ccacagatcc agtccggact cgacggcgta cttgtcgacc
     7441 tccgccagct cgtccgcggt cagcggtgcc gagtccagcg cggcgaggtt ctcctccagc
     7501 tgctcgacag agctcacacc gatcaacgcg gaggtgaccc tcgggtcccg cagcacccag
     7561 gcgagcgcca gctgcgagag cttctggccg cgccgctgag cgatcccgtt cagcccgcgg
     7621 acccggtcga gattctcctc cgacaggtgc tcctgatgca acgacccctg ctgcgtggcg
     7681 cgcgatccct cgggcacgcc gttgaggtac ctgtctgtga gcaggccctg gccgagcggg
     7741 gagaacgcga tgcagcccgc gccgacctcc gcgagggtgt ccagcagttc cggctcgatc
     7801 cagcggttga gcatcgagta cgacggctgg tggatcagca gcggcacgcc cgcctgcctg
     7861 agcaggtccg cggcctcctt ggtcttggca ggcgagtagg aggagatgcc gacgtagagg
     7921 gccttgccct gctgcaccgc gctgaccagc gcgcccaccg tctcttccag cggtgtgtcc
     7981 gggtcgaacc ggtgcgagta gaagatgtcg acgtagtcca gccccatccg gccgagcgac
     8041 tggtccagcg aggccagcag gtacttgcgc gagccgaagt caccgtacgg tcccggccac
     8101 atgtcgtagc ccgccttggt ggagatcagc agctcgtcgc ggtacggccg caggtcgtcg
     8161 cgcaggatcc ggccgaagtt ctcctcggcc gagccgtagg gcgggccgta gttgttggcc
     8221 aggtcgaagt gcgtcacgcc gaggtcgaac gcgcgccggg cgatctgcct gccgtgctcg
     8281 tacggccggg aaccgccgaa gttctgccac agccccagtg agatcagcgg cagtttgagg
     8341 ccgctgcgcc cgctgcgccg gaattccgca ttgtcgtatc gccgctcgtc ggctcggtac
     8401 accacggtcg cgaatatagg tcaacagtgc cgcgtgatcc cagtcccttg gccttgctgg
     8461 ggctcaaatc gtccctggcg gcaccggccg ggaagtccaa agacaaccag tattcgcgac
     8521 ttcccggcgc caccgccagg aacaatttga tctcccagca aggcgaaagg gactgggatc
     8581 acgcggcacg aggcgaggtg gcccggcaat gggggcaagg ccaccccgac ctcaaccgcc
     8641 gatgtacccg cgctgggccg tggccctgtg ccgccctcgg acgacaactt cggacacctc
     8701 ttcggcacgg accacatagg gccgcgcgcg gacatcggcg gagtcccacc tcggcacgat
     8761 cggacgtgcg tacatgtcga tcgagtccga gcagccgggc agccacagcc acccatagcc
     8821 cttgaattcc ttctcccgca cggcaagcct gcggaactgg tcggtgcgca gcgacgtgtg
     8881 ccccgcctcg accaccgagc ggtagaccag ttcggacagc atcaccgcga ggttggcctc
     8941 cggccactgt gccagcacac cgcgcagtgg cttcgagtcg agcagcctgc cgaccaccac
     9001 cgcgtccctg ccgggaaatc cgttggggcc gttgacgacc acgccgtggt gcacggccat
     9061 ccgcaaccgc agcctcgcac agggcaacac gtccgcgttg tgctggcgca gttcggcttc
     9121 cagccgccgg gtgaacccgt cgacgatgac cggctcgggc tcgtgatggg gaacgaccgc
     9181 cagttccgcg tcgccctgct gctgacgggt ccagtgcgcc cggttcaggc cgaccgacga
     9241 cgccgcccga tcgagcgacc gcagcaggat ctcctgccat ttctgttgac gctgctccgg
     9301 tgcggcgccg tacccacgaa tatcaactcc gacatagagc gaccgtcgga agccggtatc
     9361 tggcatacaa ttagaccctt cgagtacgga tccgagggat gatggtgctc tgtgtaagca
     9421 tgcagtagtt cgtccggctg aaagcggcac tggtacgggt tcgccagctt ttcaccgaat
     9481 acccgggtcc gattggacca attttttaac agtacaaggg tcacgttatt tcggcgcgca
     9541 ccctgagcct ggccatatcg agcacggcga tgccccggta tcgcccgctg accaggcctt
     9601 cctgctggaa ggcgcgaatt atcttctccg ctgtcgacag cttgataccg gcgatcgacg
     9661 cgagctcgtc ccttgtcaac ggaacggtca gatcccaatg accactcgat cttgttccat
     9721 atgcctcgta aagatcgacg agaacacgcg cgacgcgcac gctcgccgga cggccgtgtt
     9781 cgattcttcg gcggtcggcc caccgcaatc gttcgctcaa catggtggcc acctcgatgg
     9841 ccgcgtccgg gcgcctggcg aggaagttgc gcagctccgc cttggtcatc cgcttgtaga
     9901 aaatgtcggt gcacgcggtc accgtcgccg atctcgggcc gacgtcgagc gcggccatct
     9961 cgccgacgag atcaccaccg accctgatcg cgagcagtgt ccgttccccg gccgggtccg
    10021 tggccagcac tttcaccgac ccgtgcagca acagcagtgc cgatgaatcg ttttcaccct
    10081 ggttgatgat tgtcacgccg gccggtcggc tttccgaaac gccgacggcg aggaaatcct
    10141 ccctcgtccg ttccgacagc cgcccgagca ggctcgtctg tgggtacggc agtgccccac
    10201 cggccactct cctaggaatg atctttccca cctcgccgat tgtcgagccg gcccgagaat
    10261 gcgaccaccg gacccatcga cgcgtttccg cggactgtcc gcaatagtgg cacacggata
    10321 aagcggctca gttggccagg tgctcgacca ggaaaacctg cagctcgccg acggcgaagc
    10381 cgagcacggc gccgac
//
