LOCUS       NZ_CDME01000006        10306 bp    DNA     linear   CON 13-AUG-2024
DEFINITION  Kibdelosporangium sp. MJ126-NF4, whole genome shotgun sequence.
ACCESSION   NZ_CDME01000006
VERSION     NZ_CDME01000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Kibdelosporangium sp. MJ126-NF4
  ORGANISM  Kibdelosporangium sp. MJ126-NF4
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Kibdelosporangium.
REFERENCE   1
  AUTHORS   Ramaraj,Thiru.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2014) National Center for Genome Resources (NCGR),
            National Center for Genome Resources (NCGR), 2935 Rodeo Park Drive
            East, Santa Fe, New Mexico, 87505, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000826545.1-RS_2024_08_13
            Annotation Date                   :: 08/13/2024 03:10:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,648
            CDSs (total)                      :: 10,573
            Genes (coding)                    :: 10,470
            CDSs (with protein)               :: 10,470
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 19 of 103
            Pseudo Genes (incomplete)         :: 89 of 103
            Pseudo Genes (internal stop)      :: 5 of 103
            Pseudo Genes (multiple problems)  :: 10 of 103
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:05:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 735902
            Orig. end                         :: 746208
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CDME01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10306
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="2"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10306
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10306
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5306](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5306)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    complement(299..315)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 20.44"
                     /tool="antismash"
     misc_feature    344..360
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.02"
                     /tool="antismash"
     gene            369..1007
                     /locus_tag="BN1199_RS17700"
     CDS             369..1007
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /locus_tag="BN1199_RS17700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_042184536.1"
                     /transl_table=11
                     /translation="MTRRNAKYDAIVRASRAVFGRVGYLTASIDVIAADAGVSTRTIYN
                     HFDNKEHLFAEVLLASSRQVAEVHEAIIERNLADVTDITDVEAALRGLAMEWDQPQPEF
                     DDHFAITRRMDAEREHFPAEIYRGWREAGPWRVQRALAVRFEQLRDKGLLDFPDAQFAA
                     HHFSVLLWATARTRGRDKDGPLAAKDFERIAADVVPAFLYGCAARRSGG"
     gene            complement(988..1323)
                     /locus_tag="BN1199_RS17705"
     CDS             complement(988..1323)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230600.1"
                     /locus_tag="BN1199_RS17705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_042184539.1"
                     /transl_table=11
                     /translation="MAQHAESKGSPESSGVTRGQVVGVIAAVVRWVGLLCTLILVIHVL
                     LTVGSANPANGITSFFADLAEPIALGFKDLFTPSDPKLHVLVNYGIAALFWLIVSSVVA
                     RIIRRFA"
     gene            complement(1378..1677)
                     /locus_tag="BN1199_RS17710"
     CDS             complement(1378..1677)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683932.1"
                     /locus_tag="BN1199_RS17710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_042184540.1"
                     /transl_table=11
                     /translation="MTTRRWHVSSGRGTAARIVFGVTAIFALIEAVYILLIVLGANTAN
                     GFFTFIQSLAEPLALFFPGLFATGNYNLDVILNYGLAAVFWLVIGSIIARIIGR"
     gene            1913..3697
                     /gene="leuA"
                     /locus_tag="BN1199_RS17715"
     CDS             1913..3697
                     /EC_number="2.3.3.13"
                     /GO_function="GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="leuA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271: 2-isopropylmalate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097697.1"
                     /locus_tag="BN1199_RS17715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2-isopropylmalate synthase"
                     /protein_id="WP_042184931.1"
                     /sec_met_domain="HMGL-like (E-value: 4.1e-79, bitscore:
                     261.3, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTNPDFRTSRIRKPARPAPAGQPEWNAQLGSSMPVHRYRPFAEL
                     VEDVTLTDRTWPDRRITQAPLWCAVDLRDGNQALIDPMSPARKRRMFDLLVKMGYKEIE
                     VGFPAASQTDFDFVREIITEGAVPDDVRIQVLSQCRPELIERTFEALQGVSKAIVHIYN
                     STSILQRRVVFREEREGIKKIATSAAEMVVELADKYTDTDFRFQYSPESYTGTELSYAA
                     EVCNAVTEIWKPTPQRPAILNLPATVEMATPNVYADSIEWMHRNLERRDSVILSLHPHN
                     DRGTGVAAAELGYQAGADRIEGCLFGNGERTGNVCLVTLGMNMFSQGIDPQIDFSDIDE
                     VRRTVEYCNQLPVPERHPYGGDLVFTAFSGSHQDAINKGLDALRDAADKAGTPVDQFEW
                     EVPYLPIDPKDVGRTYEAVIRVNSQSGKGGVAYVMKTEHQLELPRRLQIEFSGVVQRMT
                     DTEGGEVSPAQMWDMFATEFLTATAPLTLQRHRLADDGNGHDEISAVVHVEGDAHEVTG
                     NGNGPIAAFVDALASVGYDVRVLDYTEHALTAGDDARAAAYVECAVGDRVLWGVGVDSS
                     IVTASLRAVVSAINRANR"
     gene            complement(3678..4001)
                     /locus_tag="BN1199_RS17720"
     CDS             complement(3678..4001)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BN1199_RS17720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_042184541.1"
                     /transl_table=11
                     /translation="MSAVIRPPEAATPIFRNGFATTALALGIVGVGFGLTVLFGLLAAV
                     FGALAVLFGIVGVVRARKGRATDGGMAAIGVVAGLIAVALGIAGMVSMPDQPDSLSSTG
                     LLG"
     gene            complement(4170..4985)
                     /locus_tag="BN1199_RS17725"
     CDS             complement(4170..4985)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101895.1"
                     /locus_tag="BN1199_RS17725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_042184542.1"
                     /transl_table=11
                     /translation="MGVRKNQQNLTAGEKTRLVDAMLAMKRSGAYDSFVRQHIERLNGD
                     SDNGTRIGHRSPSFLPWHRKYLIEFERALQAVDSTVSLPYWDWTTAGARSTLWATDFMG
                     PDGSRTQNGRVTGGPFTGTNWPLSVRSDSAGYLRRSLGSGGVTLPTAGDVRTVLGIRTY
                     DTAPWNSTSSGSFRNSMEGFRGPNLHNRVHVWVNGTMAGAGSPNDPVFWLHHCHVDKLW
                     SDWQAGNGRANYVPVSGTADVVDIDEPMEPWGDVTPRDMFDHTAVYTYE"
     gene            complement(5001..5306)
                     /locus_tag="BN1199_RS17730"
     CDS             complement(5001..5306)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017995.4"
                     /locus_tag="BN1199_RS17730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_198151531.1"
                     /sec_met_domain="melC (E-value: 6.4e-22, bitscore: 73.6,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLNRRRLLMTSAVALAGGVTIGWNVLVANADQEFDETYEGRRING
                     RADMLFIDGDLVHVMRNGDGTYTTSINHYESFTSLREAAKRAVDTLGGLRPVSHAH"
     misc_feature    complement(5374..5387)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     18.15"
                     /tool="antismash"
     gene            complement(5407..6282)
                     /locus_tag="BN1199_RS17735"
     CDS             complement(5407..6282)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /locus_tag="BN1199_RS17735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_042184546.1"
                     /transl_table=11
                     /translation="MELGQGHLKTIIAVAECGSIGRAAVRLGQSQPAVSGNVRRIEKYF
                     GGALFHRSATGVRTTPLGDWVLVKARTLAADMEQLVSSARTVAGGTDGLTVAGQPCATM
                     VALIPLLPREAEVRVDHSAGWLLQQLADDAVDVCVLTEPAGAELPVPDGVHRKVLAREP
                     CPVGLSAEHPLAGRETVELTDLAEDDWVDNPFSDSGLRSACRRAGFEPRVRYWIADPGV
                     YWPLVRSGRAVGLFTGATHARHGVVFRSLQGDPLARRIVLYWRDRAAGHAALIQRLTGV
                     TMINGTESPY"
     misc_feature    complement(5449..5451)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6356..7381
                     /locus_tag="BN1199_RS17740"
     CDS             6356..7381
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875125.1"
                     /locus_tag="BN1199_RS17740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_042184548.1"
                     /transl_table=11
                     /translation="MVATEDRPTLEDVASHAGVSRSTASRALNAEMYVSSKTREAVLLA
                     ARDLGYSPNQAARSLVTRRTGAVALVLSEPEAKVLEDPYFLIVMRAAFRELAGRGSQML
                     VMFVDAPDDIPRTMGFLDGGHVDGALVFAPHRGDPLPIALKQLRVPVVFGGRPSHSSAD
                     LYSVDYDNVTGARLAVEHLLHLGRKKIVTIAGPSSHPAAVDRVTGWRETLAKQGISVNG
                     LAEDGDFTQAGGERAMATLLERESDLDAVFAASDPMAIGALHALRKAGRRVPEDVAVVG
                     FDDNPSLAPVTDPPLTSVHQEPEEQVRQMIVMLHDLLAGQKPDTRRKILPVTLTIRTST
                     S"
     gene            complement(7438..8469)
                     /locus_tag="BN1199_RS17745"
     CDS             complement(7438..8469)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676167.1"
                     /locus_tag="BN1199_RS17745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_042184552.1"
                     /transl_table=11
                     /translation="MSELTTSPTKTETLLQASGLQKYFPIRKGLLQRVKGNVQAVDGLD
                     FSVQKGETLSLVGESGCGKTTTGRLLTRLLEPTAGKVVFEGQDITHMSQAAMRPLRRDV
                     QMIFQDPYSSLNPRHTVGAIVGAPFNLQKVKPEKGVKKSVQELLELVGLNPEHYNRYPH
                     EFSGGQRQRIGIARTLALKPKLIIADEPVSALDVSIQAQVVNLLEDLQNELDLTYVMIA
                     HDLSVVRHVSDRVAVMYLGKIVELADRTSLYEKPMHPYTVALLSAVPVPDPERKAKRER
                     IRLKGDVPSPINPPSGCRFHTRCWKAQEICKTQEPQLVELAPGRQAACHFPENTGSQTI
                     AVI"
     gene            complement(8474..9553)
                     /locus_tag="BN1199_RS17750"
     CDS             complement(8474..9553)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660002.1"
                     /locus_tag="BN1199_RS17750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_083466492.1"
                     /transl_table=11
                     /translation="MKHSQEDLLAGGAARPSNRPDEAFLQVRDLRVHFPTDDGIVKSVD
                     GLTFSLERGKTLGIVGESGSGKSVTSLSIMGLHKKGTADISGQILLDGQDLVTATSEHV
                     RALRGKRMAMIFQDPLTAMHPYYTVGAQIIEAYRVHHNVSKSVAKKHAVEMLDRVGIPQ
                     PDKRVDDYPHQFSGGMRQRAMIAMALSCDPELLIADEPTTALDVTVQAQILDLIVDLQK
                     DFNSAVVIITHDLGVVAELADDIMVMYAGRSVEYGSAREVFSAPQHPYTWGLLGSMPRL
                     DRERTERLQPIKGTPPSLINVPEGCPFNPRCAFAGLNGDLSTTERPEFREVSPGHHIAC
                     HLSGEQRQHLWTTEIKPKL"
     misc_feature    9549..9564
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.62"
                     /tool="antismash"
ORIGIN
        1 gacgaacgcc ttgagggaag ccgggaagct gtggttgtac tcgggcgtga cgacgacgaa
       61 cgcgtccgcc gaggccagca cttccgtcac cgcctgcagt tcgtcggacc ttgtcggctc
      121 tgtgagcgcg ggcggctcgg cgggcagcac caacggcaaa ggtacgtcgg cgaggtcgat
      181 cacctgtaca tcgaactggc cgtgtcgagt ggcgcggtcg gtgaaccagt ccgcgatcag
      241 cggcccgcgg cggccctcac ggacgctgcc cacgatcacg gcgagcttca tctcagtcat
      301 gaaggtaaag tacaacgtgc atggcaattt agtaaaacga aggttgtaac tttgctgaca
      361 ggacgactgt gaccaggcga aacgcgaagt acgacgcgat cgtgcgggca tcgagggcgg
      421 tgttcggcag ggtcggctac ctgaccgcga gcatcgacgt gatcgcggcc gacgccggtg
      481 tctcgaccag aacgatctac aaccacttcg acaacaagga gcacctgttc gccgaggtgc
      541 tgctggccag ttcgcgccag gtcgccgagg tgcacgaggc gatcatcgaa cggaacctgg
      601 ccgacgtcac cgacatcacc gacgtggagg cggccctgcg cggcctggcc atggagtggg
      661 accagccgca gccggagttc gacgaccact tcgcgatcac ccggcggatg gacgccgagc
      721 gggagcactt cccggcggag atctaccgcg gctggcgcga agcgggtccg tggcgcgtcc
      781 aacgcgcact ggccgttcgc ttcgaacagc tgcgggacaa gggattgctg gacttcccgg
      841 acgcgcagtt cgccgcgcac cacttcagcg tgctgctgtg ggcgaccgcg cgcaccagag
      901 ggcgggacaa ggacggaccg ttggcggcca aggacttcga gcggatcgcg gccgacgtcg
      961 tgcccgcgtt cctctacggc tgtgcggcta ggcgaagcgg cggatgatcc tggcgaccac
     1021 actggacacg atcagccaga acagcgccgc gatgccgtag ttgaccaaca cgtgcagctt
     1081 ggggtcgctc ggcgtgaaca ggtccttgaa gccgagcgcg atcggttcgg cgaggtcggc
     1141 gaagaagctc gtgatcccgt tggccgggtt ggcactgccg acggtcagca gcacgtggat
     1201 caccaggatc agcgtgcaga gcaggccgac ccacctgacc acggcggcga tgacgccgac
     1261 cacctgtccc cgcgtgacgc ctgagctctc cggcgagcct ttggattcgg catgctgggc
     1321 catgcctcgg agtctggcat ggcccggcag ctttggctac ttctgagtaa caaactgtta
     1381 tcggccgatg atccgggcga tgattgagcc gatgaccagc cagaacaccg ccgcgaggcc
     1441 gtagttgagg atcacgtcga ggttgtagtt cccggtggcg aacaggccgg ggaagaacag
     1501 cgcgagcggc tccgcgagcg actggatgaa cgtgaagaac ccgttcgcgg tgttcgcgcc
     1561 gagcacgatc aacaggatgt acacggcttc gatcagcgcg aagatcgccg tgactccgaa
     1621 cacgatccga gccgccgtcc cccgtccgct cgagacatgc catctgcgag ttgtcatatc
     1681 tctgggttga cctcggtgcg cgccgcgaaa cgctgccgtt cggagtaacc cgttcgccca
     1741 gcatgtggga cgcccggttt ccggacaccg gggtcggctc gctagtgtgg ggccgtggct
     1801 cgatctctcc tgcttcgctg ccgcgacggg gcctgacctt cttgaccggc tccccgccgc
     1861 ggagctcagt gttgccgccg gtcgtccgct cgtgaagctg gagacccttt cgatgaccac
     1921 gaatcccgac tttcgcacgt cccgcatccg caaaccggcg cggcccgcgc ccgccggtca
     1981 gccggagtgg aacgcccagc tcggctcgtc gatgccggtg caccgctacc ggccgttcgc
     2041 cgaactggtc gaggacgtca cgctgaccga ccgcacctgg ccggaccgca ggatcaccca
     2101 ggccccactg tggtgcgcgg tcgacctgcg ggacggcaac caggcgctga tcgacccgat
     2161 gtcgcccgcc cgcaagcggc ggatgttcga cctgctggtc aagatgggct acaaggagat
     2221 cgaggtcggc ttcccggcgg ccagccagac ggacttcgac ttcgtccgcg agatcatcac
     2281 cgagggcgcg gtccccgacg acgtccgcat ccaggtgctg tcgcagtgcc gtcccgaact
     2341 gatcgagcgg acgttcgaag cgctgcaggg cgtgagcaag gcgatcgtcc acatctacaa
     2401 ctccacctcg atcctgcagc gccgcgtggt gttccgcgag gagcgcgaag gcatcaagaa
     2461 gatcgccacc tcggccgcgg agatggtcgt cgaactggcc gacaagtaca ccgacacgga
     2521 cttccgcttc cagtactcgc cggagtccta caccggcacg gaactgtcct acgcggccga
     2581 ggtctgcaac gcggtcaccg agatctggaa accgacgcca cagcgcccgg cgatcctgaa
     2641 cctgcccgcg accgtggaga tggccacgcc caacgtgtac gcggactcca tcgaatggat
     2701 gcaccgcaac ctggagcgcc gcgactcggt gatcctgtcg ctgcacccgc acaacgaccg
     2761 cggcaccggt gtggccgcgg ccgaactggg ctaccaggcc ggtgcggacc gcatcgaggg
     2821 gtgtctcttc ggcaacggcg agcgcaccgg caacgtctgc ctggtcacgc tgggcatgaa
     2881 catgttcagc cagggcatcg acccgcagat cgacttctcc gacatcgacg aggtccggcg
     2941 gacggtcgag tactgcaacc agttgccggt acccgaacgt cacccctacg gcggtgacct
     3001 ggtgttcacc gcgttctccg gcagccacca ggacgcgatc aacaagggcc tcgacgcgct
     3061 gcgcgacgcg gccgacaagg cgggcacccc ggtcgaccag ttcgagtggg aggtcccgta
     3121 cctgccgatc gacccgaagg acgtcggccg cacgtacgag gccgtgatcc gggtgaactc
     3181 gcagtccggc aagggcggcg tcgcctacgt gatgaagacc gagcaccagc tggaactgcc
     3241 gcgcaggctg cagatcgagt tctccggcgt ggtgcagcgg atgaccgaca ccgaaggcgg
     3301 cgaggtcagc ccggcccaga tgtgggacat gttcgcgacc gagttcctga cggcgacggc
     3361 accgctgacg ttgcagcggc accggctggc cgacgacggc aacggccacg acgagatctc
     3421 cgcggtcgtg cacgtcgaag gggacgcgca cgaggtcacc ggcaatggca acggcccgat
     3481 cgcggcgttc gtcgacgcgc tggcgagcgt gggctacgac gtccgcgtcc tggactacac
     3541 cgagcacgcg ctcaccgcgg gcgacgacgc acgcgccgct gcctatgtgg aatgcgccgt
     3601 gggagacagg gtgctgtggg gcgtcggcgt cgactcgtcg atcgtgacgg cctcgctgcg
     3661 tgccgtcgtg tccgcgatca accgagcaaa ccggtagaac tcagcgagtc gggttggtcg
     3721 ggcatggaca ccatgcccgc aatcccgagt gccaccgcga tcagaccggc gacgacaccg
     3781 atggcggcca tcccgccatc ggtggctcgg cccttgcggg cccgcacgac gccgacgatt
     3841 ccgaacagca cagcgagcgc cccgaacacg gccgcgagca ggccgaacag gacggtcagt
     3901 ccaaacccga cgccgacgat gcccagcgcc agtgcggtcg tggcgaaccc gttgcggaag
     3961 atcggcgttg cggcctcagg cggccggatc acggcgctca tgggcgactc ccgtggggaa
     4021 ggcgtccggg ctggacgctt ggcccataaa tcgccgttat ccaccttcgt gttacccgtt
     4081 gatgctcgtg tcaacctatt ggcgtacaac ggaaaacacc ctgtgtgcga gtcattgcac
     4141 agcgttgtga cttgaccgat tccgtcggat tactcgtacg tgtagacggc cgtgtggtcg
     4201 aacatgtccc tcggtgtcac gtcgccccac ggctccatcg gctcgtcgat gtccacgacg
     4261 tcggcggtgc cggagaccgg aacgtagttc gccctgccgt ttccggcctg ccagtccgac
     4321 cacagcttgt ccacatggca gtgatgcagc cagaacaccg gatcattcgg tgatcccgcg
     4381 cctgccatgg tgccgttcac ccacacgtgc acccggttgt gcaggttcgg gccgcggaag
     4441 ccctccatgc tgttgcggaa gctgcccgaa gacgtgctgt tccacggcgc ggtgtcgtat
     4501 gtcctgatgc cgaggacagt ccggacatcg ccggctgtcg gcagtgtgac cccgccggag
     4561 ccgaggctgc gccgcaggta cccggccgag tcgctgcgga ccgacagcgg ccagttcgtg
     4621 ccggtgaagg gaccgccggt gacccggccg ttctgggtcc ggctgccgtc cgggcccatg
     4681 aagtccgttg cccacaaggt ggatcgggcg cccgcggtgg tccagtccca gtacggcagg
     4741 ctgaccgtcg agtcgacggc ctgcagcgcc cgttcgaact cgatcaggta cttgcggtgc
     4801 cacggaagga aggacggcga acggtggccg atcctggtgc cgttgtcgct gtcgccgttg
     4861 agccgttcga tgtgctggcg gacaaaggag tcgtacgctc ctgagcgttt catcgccagc
     4921 atggcgtcca cgaggcgggt cttctcgccc gccgtcaggt tctgctggtt cttgcgaacc
     4981 cccattggtg ctcccccgtt tcagtgcgcg tgtgagaccg gacgcaggcc gccgagcgtg
     5041 tcgaccgccc gttttgccgc ctcgcgcaac gacgtgaacg actcgtagtg gttgatcgac
     5101 gtggtgtacg tgccgtcgcc gttgcgcatc acgtgcacga ggtcgccgtc gatgaacaac
     5161 atgtccgcgc ggccgttgat ccggcggccc tcgtaggtct cgtcgaattc ctggtccgcg
     5221 ttggcgacca gcacgttcca gccgatggtg acgccaccgg ccagggcgac cgcgctggtc
     5281 atcaacagtc ttcgtctgtt caacatgccg cagaagctag ccgcgccgaa gaccggacgg
     5341 ttccgccgat cgggaggaaa catgtgccca taggcaaagg cgatgcctga atctccagat
     5401 agccgtctag taagggcttt ctgtgccgtt gatcatagta acgccggtta accgctggat
     5461 caaggccgcg tgtcccgccg cccggtcgcg ccagtacagg acgatccgcc tggccaacgg
     5521 atctccttgc agtgaacgga aaacaacgcc atggcgggca tgtgtcgccc cggtgaacaa
     5581 gccgacagcg cgaccggaac ggacgagggg ccagtacacc ccggggtcgg cgatccagta
     5641 ccggacgcgc ggctcgaacc cggcgcggcg gcaggctgag cgcagaccgc tgtcgctgaa
     5701 cgggttgtcc acccagtcgt cttcggcgag gtcggtgagt tcgacggttt ccctgcccgc
     5761 caacggatgc tccgccgaca gcccgaccgg acacggctcg cgggcgagca ccttcctgtg
     5821 cacgccgtcc ggtacgggca gttcggcccc ggcgggttcg gtcagcacgc agacgtccac
     5881 cgcgtcgtcc gccagctgct ggagcagcca cccggccgaa tggtccaccc gcacctcggc
     5941 ctcgcggggc agcagcggga tcagcgccac catcgtggcg cacggctggc ccgcgacggt
     6001 cagcccgtcg gtcccgcccg ccacagtgcg ggccgaggac accaactgct ccatgtcggc
     6061 ggccagtgtg cgggccttca ccagcaccca gtcgcccagt ggcgtggtcc gtacgccggt
     6121 ggccgaccgg tggaacaacg ccccgccgaa gtacttctcg atccgcctga cgttgccgct
     6181 gacggccggc tggctctggc cgaggcggac ggccgccctg ccgatactgc cgcactcagc
     6241 caccgcgatg atcgtcttca aatgcccctg tcccaactcc ataatgtgga gatcttctcg
     6301 tgaccagggc tttccacaac agtatctttc gacctatctg ccctatgctc tgcagatggt
     6361 ggcaaccgag gaccgtccga cgctcgagga cgtcgcgtca cacgcgggcg tttcccgctc
     6421 gacagcgtcg cgcgcgctga acgcggagat gtacgtcagc tcgaagacac gggaggcggt
     6481 gctgctcgcg gcgcgtgatc tcggctactc cccgaaccag gcggcccggt cgctcgtcac
     6541 caggcggacc ggggcggtcg cgctggtgct gtccgagccc gaggccaagg tgctcgagga
     6601 cccctacttc ctgatcgtga tgcgcgccgc gttccgcgaa ctcgccgggc ggggcagtca
     6661 gatgctggtg atgttcgtcg acgcgccgga cgacatcccc cgcacgatgg gcttcctcga
     6721 cggcgggcac gtcgacggcg cgctggtgtt cgcgccgcac aggggagatc ccctgccgat
     6781 cgcactgaaa cagttgcggg tcccggttgt cttcggtgga cggccgagtc attcgagcgc
     6841 tgatctgtac tcggtggact acgacaacgt cacgggtgcg cggctggcgg tcgaacacct
     6901 gcttcacctg gggcgcaaga aaattgtcac catcgccggt ccgtccagtc acccggcggc
     6961 cgtcgaccgg gtcaccggct ggcgggaaac ccttgccaag caagggatat cggtcaacgg
     7021 cctggccgag gacggcgact tcacgcaggc cggcggcgaa agggcgatgg ccacgctgct
     7081 ggagcgcgag tcggacctcg acgccgtgtt cgccgcgagc gacccgatgg cgatcggagc
     7141 gctgcacgcg ttgcggaaag cgggcagacg agtgcccgag gacgtcgcgg tcgtgggatt
     7201 cgacgacaac ccgtcgctgg cgccggtcac ggacccgccg ctgacctcgg tacaccagga
     7261 accggaggaa caggtccggc agatgatcgt gatgctgcac gacctgctgg ccggacaaaa
     7321 accggacacc cggcgcaaaa tcctgccagt aaccctgaca atccgcacct caacctcgtg
     7381 agtgtttggt ccggttagaa ccggatcaaa cactcacgaa gctgacgctg ggaagtttca
     7441 gatcacggcg atggtctgcg acccggtgtt ctccgggaag tggcacgcgg cctgacggcc
     7501 gggggccagc tcgaccagct ggggctcctg ggtcttgcag atctcctgtg ccttccagca
     7561 gcgcgtgtgg aaccggcagc cggacggcgg gttgatcggc gacggcacgt cacccttcaa
     7621 ccggatccgc tcccgcttgg ccttgcgctc cgggtccggc accggcaccg cggacagcag
     7681 cgccacggtg tacgggtgca tcggcttctc gtacaggctg gtccggtcgg ccagctcgac
     7741 gatcttgccg aggtacatca ccgcgacccg gtcggacacg tgccgcacca ccgacaggtc
     7801 gtgcgcgatc atcacgtacg tcaggtcgag ctcgttctgc agatcctcga gcaggttcac
     7861 cacctgcgcc tggatcgaga cgtccagagc ggacaccggc tcgtcggcga tgatcagctt
     7921 cggcttcagc gcgagcgtgc gggcgatccc gatgcgctgc cgctggccgc cggagaactc
     7981 gtgcgggtag cggttgtagt gctcggggtt caggccgacc agttccagca gctcctggac
     8041 ggacttcttg acgcccttct cgggcttgac cttctgcagg ttgaacggcg cgccgacgat
     8101 cgcgcccacg gtgtgccgcg ggttcagcga cgagtacggg tcctggaaga tcatctgcac
     8161 gtcccggcgc agcgggcgca tggccgcctg cgacatgtgc gtgatgtcct ggccctcgaa
     8221 gacgaccttg cccgcggtcg gttcgagcaa ccgggtcagc agccgccccg tcgtggtctt
     8281 gccacagccg gactcgccga ccagcgagag cgtctcgccc ttctgcaccg agaagtccag
     8341 cccgtccacg gcctgcacgt tgcccttgac gcgctgcagc aggcccttgc ggatcgggaa
     8401 gtacttctgc agaccgctcg cttgcagcag cgtctcggtt ttggtggggg aggtggtcag
     8461 ttcactcatc agctcacaac ttcggcttga tctcggtggt ccacaggtgc tggcgctgct
     8521 cgccggacag gtggcacgcg atgtggtggc cggggctcac ttccctgaac tccgggcgct
     8581 cggtggtgga gaggtcgccg ttgagccccg cgaaggcaca gcgcgggttg aacgggcagc
     8641 cttccggcac gttgatcagg ctgggcggtg tgcccttgat cggctgcagc cgctcggtgc
     8701 gttcccggtc cagccggggc atcgagccga gcagacccca ggtgtagggg tgctgcggcg
     8761 cggagaagac ctcgcgcgcg ctgccgtact ccaccgagcg gcccgcgtac atcaccatga
     8821 tgtcgtcggc cagctcggcg accacgccga ggtcgtgggt gatgatgacg accgcggagt
     8881 tgaagtcctt ctgcaggtcc acgatcaggt cgaggatctg cgcctgcacg gtcacgtcga
     8941 gcgcggtggt cggctcgtcc gcgatcagca gctccgggtc gcatgacagc gccatcgcga
     9001 tcatcgcgcg ctggcgcata ccgccggaga actggtgcgg gtagtcgtcc acgcgcttgt
     9061 ccggctgcgg gatgccgacg cggtcgagca tctcgaccgc gtgcttcttg gccaccgact
     9121 tggacacgtt gtggtgcacc cggtaggcct cgatgatctg cgcaccgacc gtgtagtacg
     9181 ggtgcatcgc ggtcagcggg tcctggaaga tcatcgccat ccgcttgccg cgcaacgccc
     9241 gcacgtgctc cgacgtggcg gtgaccaggt cctggccgtc cagcaggatc tggccggaga
     9301 tgtccgcggt gcccttcttg tgcaggccca tgatcgacag gctggtcacg ctcttgccgg
     9361 agccggactc gccgacgatg ccgagggtct tgccccgctc gagcgagaac gtcagcccgt
     9421 ccaccgactt gacgatgccg tcgtcggtcg ggaagtgcac cctcaggtcc cgaacctgca
     9481 ggaaagcctc gtccggacgg ttcgacgggc gggccgcgcc gcctgccagc aagtcctctt
     9541 gggaatgttt cacgagtact tcaccctcgg gtcgacgacg gcgtacaaca ggtcaacgac
     9601 caggttcgcg atgacgatga aggtcgcgcc gaccagcgtc acgcccagca ccttcggaag
     9661 gtcgttgttc acgatcgcgt caatggcgta cttgccgagg ccgggcagcg agtacgtgct
     9721 ctcggtgagc accgcgccgc cgagcaggat gcccaggtcg agaccgaaga tcgtgaggat
     9781 cggggtgagc acggaacgca ggccgtgctt gacgaccacg gtccgttcgt tgaggccctt
     9841 cgcccgcgcg gtgcggatga agtcctcgtt catcgtctcc agcatggtcg ccctggtcag
     9901 ccgggcgtac ccggccgcgt tcaggaacgc cagcgtgatc cacggcagga tcagcgcacc
     9961 cgcccacgcc agtgggttgt cgccgaagtt gacgtacgta ccaccctgca cggtccagcc
    10021 gaggccgtag ctgaagatcg tcagcgacag caggccggtg aagaagatcg gcagcgacac
    10081 cccggccagc gcgacaccca tcgcggcccg gtcgaagatc gtgccgcggc gcaacgccga
    10141 caccacacca gtgctgatgc cgaagaacag ccagatcacg gcggcaccga gggccagcga
    10201 gatcgtcacg cccatccggt cgaccagatc cggccagacc gggttctggt tgatgaagga
    10261 atagcctaga cagggcgccg ggcagtgcac cgtcgtcgga ccggtg
//
