LOCUS       NZ_CDME01000002        13403 bp    DNA     linear   CON 13-AUG-2024
DEFINITION  Kibdelosporangium sp. MJ126-NF4, whole genome shotgun sequence.
ACCESSION   NZ_CDME01000002
VERSION     NZ_CDME01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Kibdelosporangium sp. MJ126-NF4
  ORGANISM  Kibdelosporangium sp. MJ126-NF4
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Kibdelosporangium.
REFERENCE   1
  AUTHORS   Ramaraj,Thiru.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2014) National Center for Genome Resources (NCGR),
            National Center for Genome Resources (NCGR), 2935 Rodeo Park Drive
            East, Santa Fe, New Mexico, 87505, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000826545.1-RS_2024_08_13
            Annotation Date                   :: 08/13/2024 03:10:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,648
            CDSs (total)                      :: 10,573
            Genes (coding)                    :: 10,470
            CDSs (with protein)               :: 10,470
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 19 of 103
            Pseudo Genes (incomplete)         :: 89 of 103
            Pseudo Genes (internal stop)      :: 5 of 103
            Pseudo Genes (multiple problems)  :: 10 of 103
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:05:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 464862
            Orig. end                         :: 478265
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CDME01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13403
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="aminopolycarboxylic-acid"
                     /region_number="5"
                     /rules="(AesA and AesB and AesC)"
                     /tool="antismash"
     cand_cluster    1..13403
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(AesA and AesB and AesC)"
                     /kind="single"
                     /product="aminopolycarboxylic-acid"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13403
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8403](-)"
                     /cutoff="10000"
                     /detection_rule="(AesA and AesB and AesC)"
                     /neighbourhood="5000"
                     /product="aminopolycarboxylic-acid"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..8403)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(AesA and AesB and AesC)"
                     /neighbourhood="5000"
                     /product="aminopolycarboxylic-acid"
                     /protocluster_number="1"
     gene            complement(110..670)
                     /locus_tag="BN1199_RS02480"
     CDS             complement(110..670)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /locus_tag="BN1199_RS02480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydrofolate reductase family protein"
                     /protein_id="WP_042178407.1"
                     /transl_table=11
                     /translation="MKRAVASVHMSLDGVIEHPERWMFDHHDGEAAAYAHQQLLANDAL
                     VMGRRTYVTLAEAWSAAPTGSDMATRMNTMPKYVVSSTLDRVDWCNTTVISGDLPGEVS
                     KLLRDGQNLLMYGFGPVAQTLMYHDLLDELRIWLHPVLVGSGHLRDLLFRDGSTARLRL
                     VRTRTFTSGLVVLSYQPIAETAA"
     misc_feature    complement(659..675)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.88"
                     /tool="antismash"
     gene            795..3470
                     /locus_tag="BN1199_RS02485"
     CDS             795..3470
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /locus_tag="BN1199_RS02485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_052477660.1"
                     /transl_table=11
                     /translation="MTRTHPIEVSEREAEVLAAVGARLSNAQIANRLHISVRTVESHVS
                     SLLRKYGATDRWDLADQAQRGTPPPGHLAGLPVARTTFVGRSHECDLVVGLLADVRLVT
                     LLGPGGIGKTRLAAVVAEAVAPSFPLGGAFVDLVPVRDGFVERAVAAAIGVTERPGQTL
                     ADSIAQRLGRGRSLLVLDNCEHLIDAAAAFVERLLSTCPDVTVLVTSRERTGVPGERTV
                     PVEPLPLASDAERLFRDRATAADPGFTAGQAVVVELCGRLDGLPLAIELAAARSASLGP
                     DGLLAALDDSLLMLAGGRGPVERHRSLRAVLDWSHDLLAADERELFRRLSVFASGFDLT
                     AVAAVVRGRTRGAVADVLGRLVDKSLVVHQGGARSRWRLLETVRTFAAEQLDASGERAE
                     VRQRHLHWAAAAAAELETRLDGEWRGDFDAVVDDLRGAVAAAPGQDVVPHQLARSLGHL
                     SYARRFLHEAPDHYEEAARRALTPKQAAEDLRSAADGAHAFIDTGRAFQLLLASAEQAR
                     AAGDGNTEAIALAFAVTTANRHPAGFTVEVPHERLRALLDRATAIGDHQDRSVAAHLAA
                     ANAWNANGEKVTPEPALVPAAVATARATGDPVLISAGLDGVGIAALAAGRFREAHRVAT
                     ERMSLLDDMSRDDPYPAPEITDTYHMAATCAVAVGDLRTALSVARLAAADDLVGSQSAV
                     AMSALLPALVLSGDLDEAVRHGPVIWDVCVRADSPLAWVSPAVASVALAHGLLDDEDGF
                     LLWRARAEQVAGDAQARYLASYAAFVDARVALHTGQTDNADVLTERALVDFPRQDWHRA
                     FAHAAGAELAVASGLPDAADRLAAASATATENDWATTCLTRARGRLDHDADALTTSLAG
                     WDRIGARFEHAYTLRLSQEP"
     gene            complement(3613..5004)
                     /locus_tag="BN1199_RS02490"
     CDS             complement(3613..5004)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /GO_function="GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494607.1"
                     /locus_tag="BN1199_RS02490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MATE family efflux transporter"
                     /protein_id="WP_232327787.1"
                     /transl_table=11
                     /translation="MRRSVTTPAGGYPALVALAAPIAGIQLAQVALTSVDLAMLGLLGV
                     SAVAAGGLALLLYNQVRTMCVGMVTGVGNLVATAAGAGERRTGTDTLDDQARDEIRSLL
                     RSALLVATLAAAAGAAVLCGLALALPLLGQGPEIAALARGMMFALAGGLFPMVWLNVLR
                     QFAVGMRRPGSLLAVTLVSIAVNAGCNAAFIYGWAGLPRLGVAGVGLSTTVVQFFTLAA
                     FATTIRRDPQLRTMVSLSAWRADPAVVRRIARHGTPICFTYGSEAAMTSIATLLMGVFG
                     PAMLAASNVANQLAYIVYQCNIGLSQGSSILVSRAVGQSEPQHARTIARRALTLSWTFM
                     AVVSLGYVLAPLGMLWPFLHDETDTAVLGTASILLLFAVAQQYSKGTQNILVGLLRGLG
                     DTVSGLRCTLIGYWAVGVPVMAVCAYTFGWGGWGVWTGLCVGFAATAVLLAHRFRSALR
                     FHALS"
     misc_feature    complement(3949..3951)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5001..6071)
                     /locus_tag="BN1199_RS02495"
     CDS             complement(5001..6071)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     aminopolycarboxylic-acid: AesC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630843.1"
                     /locus_tag="BN1199_RS02495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="WP_042178411.1"
                     /sec_met_domain="AesC (E-value: 1.8e-164, bitscore: 543.7,
                     seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="PALP (E-value: 1.2e-56, bitscore: 189.2,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIVQRASDLIGDTPLLELAHTGTGTRLLLKLEQMNPTGSCKVRM
                     ARQMIIEAERDGKLRPGGHIVEPTSGNTGCGLALVALERGYRFTAVVDQHAAREKLRML
                     TAMGARLVFVDCPSDGGPSSVRRRQVAARLAAELGAYHPDQHNNPGNANGYGGLASELL
                     RQLGTVDVLVAAVGTGGSLCGTIRGLRAAGARTRSVAVEPVGSIIFGGPPGIYHQTGAG
                     SPAGFPIGSNVDRTVIDDAHRVSDADAFAGARVVAQRTGLLVGGTTGGAIHVALRRLFA
                     YPPGSTVVVLCNDAGEKYLDSVYDDGWLRDRAVLDELAQRRVERWFTTYAVSVRIAARM
                     ARSVPERVAVPVAVSA"
     gene            complement(6068..7423)
                     /locus_tag="BN1199_RS02500"
     CDS             complement(6068..7423)
                     /EC_number="5.1.1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     aminopolycarboxylic-acid: AesB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630844.1"
                     /locus_tag="BN1199_RS02500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alanine racemase"
                     /protein_id="WP_232327788.1"
                     /sec_met_domain="AesB (E-value: 1.4e-197, bitscore: 653.1,
                     seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 2.7e-18, bitscore:
                     63.2, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTALSPPLPALERPWARRLRASSMLWEIARAVGGGPFHVIHPPT
                     FAENLTGLVDALTAENTRGVVYYGKKANKASAWLRECTRPGTGVDVASVPELVHALGNG
                     VRGEAIGVTGAAKSDGLLWLGLRHHCLLAVDSADELERVAVHARRLEVTADVLLRVRPP
                     TAPTSRFGFAPDDLTAAVDRCATLGTMVALRGFSFHLDGYDPAPRAELAAHLIELCRRA
                     RTLGHPASKINIGGGIAVAYVSEEDWRRFTDSKHDSWFHGGRDPQRTYPYHQSPTGAAM
                     VTEILRHDVGGAPLAARLRDAGIDLLLEPGRALLDSAGFSVFPVQGSKDSTGHLVTTVA
                     GLSMSLSEQWKGSEFLPDPILVRTGGPRQGDTPVRTAVAGSSCLEYDVLTWRTVELPAR
                     PVPGDLLVYPNTAGYQMDKNESGFHQLPLPPKVVVDGDRWHVDTDYPIQEIP"
     gene            complement(7420..8403)
                     /locus_tag="BN1199_RS02505"
     CDS             complement(7420..8403)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     aminopolycarboxylic-acid: AesA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630845.1"
                     /locus_tag="BN1199_RS02505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ornithine cyclodeaminase"
                     /protein_id="WP_042178415.1"
                     /sec_met_domain="AesA (E-value: 2.5e-154, bitscore: 509.6,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYGPALHILTRKDLHDLELAPHEAITMVEDGYLAFASGRSQNPA
                     KLMVPMPDPTRDSVAYSMLGYDGSLEQAAFKTSYRQGSTSAERYYTTITLYDDTTGAPF
                     ALLDCHRVGATRTPASTALIARTCAKPGARSALMVGTGAQGIRTLPYLLTALPGLERLR
                     LYGTHPDGIRASIAALTDQFPDREVELVDDVEAAAADSDIVVAASGRAAHPKIRLGWLP
                     PGGLLISVASKGVQEGTLAQADYTVATSAAQVEVTGRRMAGPDGVFRIDAELPDILAGR
                     APGRRTDDDRVFAFSSGMIITDIPVAHALATRAIAAGRGREVTLWS"
     gene            complement(8649..9044)
                     /locus_tag="BN1199_RS02510"
     CDS             complement(8649..9044)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BN1199_RS02510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_232327789.1"
                     /transl_table=11
                     /translation="MRAILLALGALASLYLTVCAGDHGAHVLHPGVSAASLSFGPTGGP
                     VSADIGEVDGETRGALVHSPDGADVLTRAEDDSGALLLTLLLFVFPLPLNDRVHGGYRQ
                     PGRRKHPPPIRAGHGRAFLLTVCVART"
     gene            complement(9242..9994)
                     /locus_tag="BN1199_RS02515"
     CDS             complement(9242..9994)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /locus_tag="BN1199_RS02515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="WP_042178421.1"
                     /sec_met_domain="PhyH (E-value: 3.1e-07, bitscore: 27.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLRLRFAEHGFVRLDQSFPPELATAMADAVWSELTRTHGLSRTD
                     RSTWTVTEPRKLGSLRRKRTFDALATPAIVAAISELLGEESWKQPFSWGGPLVTFPSHG
                     QWNVPADGWHIDFPARSALRLKWLAFLAPVAAGGGGTVVLSGSHHLVERYLHRIDPADP
                     GRSSVARNAIFSADPWLRSIREPGAADQRVATLMKHGGTTVDGVDLRVVELTGQPGDVV
                     FLHPHLFHAPAPNHSQTPRLMVTGGLTG"
     gene            complement(10290..10712)
                     /locus_tag="BN1199_RS02520"
     CDS             complement(10290..10712)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /locus_tag="BN1199_RS02520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SRPBCC family protein"
                     /protein_id="WP_198151221.1"
                     /transl_table=11
                     /translation="MIVRRPAEVAFQAFTDPAVTSRFWYSKSSGPMVAGAELRWEWETY
                     GASVEVRVKEVQEGRLIRFEWGNYQQPTTVELRFTPHAEDATFVEVTESGFQGSGDDAV
                     RWLNDTVGGFSTALCAMKALLEHDIELNAGPDHHPV"
     misc_feature    complement(10678..10692)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.9"
                     /tool="antismash"
     misc_feature    10702..10715
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.87"
                     /tool="antismash"
     misc_feature    complement(10712..10725)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.7"
                     /tool="antismash"
     gene            complement(11030..11491)
                     /locus_tag="BN1199_RS55720"
     CDS             complement(11030..11491)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BN1199_RS55720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157527123.1"
                     /transl_table=11
                     /translation="MTRSTTYPVLVRRLAAGMVASGVIAATVVSAGCSSDKPISPTNPP
                     NSPPATVSSQPMPSSTGPIVTKTVADVAYQAGAVQPPDTVLRVAVGQPVKINIRADRTD
                     DITVDGYPDSNADVDSTEPEDIDFTPTHPGTVTIRLRGANVTLATVYVS"
     misc_feature    11491..11504
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.32"
                     /tool="antismash"
     gene            11738..13213
                     /locus_tag="BN1199_RS02535"
     CDS             11738..13213
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635836.1"
                     /locus_tag="BN1199_RS02535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_042178429.1"
                     /transl_table=11
                     /translation="MRTLLRGGRVIDPATGFDGTADVLVSGGVVTAVGPSLPSQPGDTE
                     IDVSGLVVGPGFIDLHSHVHTIAGQRLQAMDGVTTALDLEAGLMPIERAYAEAAAAGRP
                     LHYGFSASWGSARAHVLAGIEPDAKIDSGLAVLGNPAWQRTSSPTELNAWLSLLEGELA
                     AGALGIGVLLGYAPLTDPGEFMALARLAKQAGAPTYTHVRELVEVDPATPVDGSEEIAI
                     VAAETGAAMHHCHVNSTSGHQIDRVLAALEASRSAGSRVTVEAYPYGAGSTGIGAAFLS
                     PERLKMKGLSPSSVIMLESGERIADEGRLLQVRAEQPGAPCILEFLDERSPRDLALLRQ
                     ALAFPDSIVASDALPVYWKNGTSESTEWPLPPGGATHPRTSGTYAKTLRLMVRESEAWT
                     WLEAFRRCSYLPARVLDEVAPAARAKGRLDVGADADIVVLDPDTVTDAATYFDSTRPSV
                     GIRHLFVSGVAVVTDGNLRTDAFPGKPLRGEPR"
ORIGIN
        1 cccggcagca gcacgtggcc gtcgagtcgg tcggacaggt tccggatctg actgtccaat
       61 gtggtcggac cagctgtggt tcccatgtgt cctccttcag gacgcggcgt caggcggccg
      121 tttcggcgat cggttggtag gagagaacga cgagacccga cgtgaacgtc ctggtccgca
      181 ccaggcgcag cctcgccgtg ctgccgtcgc ggaagagcag gtcgcgcagg tgtccgctgc
      241 cgaccagcac cgggtgcagc cagatccgca gctcgtcgag caggtcgtgg tacatcagcg
      301 tctgcgcgac cgggccgaac ccgtacatca ggaggttctg gccgtcccgc agcagcttcg
      361 acacctcgcc ggggaggtca ccgctgatga ccgtcgtgtt gcaccagtcg accctgtcca
      421 gggtcgacga cacgacgtac ttgggcatcg tgttcattcg cgttgccatg tccgagccgg
      481 tgggagcggc cgaccacgcc tcggcgaggg tcacgtaggt ccggcggccc atgacgagcg
      541 cgtcgttcgc caggagctgc tggtgggcgt acgcggcggc ctcgccgtcg tggtggtcga
      601 acatccaccg ttccgggtgt tcgatcaccc cgtcgagcga catgtggacc gacgcgactg
      661 cccgtttcat gtccaacctc ctccgcttgc tgccacgaca gactgtctcg gggcccggta
      721 tccgaacatc cgtgccgata cggactcacc acggaacggc cccggatcgc ccaccgatcg
      781 gtcacactgt cgcggtgacg cggacgcacc cgatcgaagt ctccgaacgc gaggcggaag
      841 tactcgcggc ggtcggcgca cgcctgtcca acgcccagat cgccaaccgc ctgcacatct
      901 ccgtgcgcac ggtcgagagc cacgtgtcgt cgctgctgcg caagtacggc gccaccgaca
      961 ggtgggacct cgccgaccag gcccagcgcg gcacaccgcc gcccggtcac ctggctgggc
     1021 tgcccgtcgc gcgcacgacg ttcgtcgggc ggtcccacga gtgcgacctg gtcgtcggtc
     1081 tgctcgccga cgtgcggttg gtgacgctgc tgggccccgg cgggatcggc aagacccggc
     1141 tggcggcggt ggtggctgag gcggtggcgc cgtcgttccc gctcggcggc gcgttcgtcg
     1201 acctcgtgcc ggtgcgcgac gggttcgtcg aacgggcggt ggccgccgcc atcggtgtga
     1261 ccgaacggcc cggccagacc ctggcggaca gcatcgccca gcggctcggc cgcggccggt
     1321 cactgctcgt cctggacaac tgcgaacacc tcatcgacgc cgcagccgcg ttcgtcgagc
     1381 ggttgctgtc gacctgtccc gacgtgaccg tgctggtcac cagccgggaa cggaccgggg
     1441 tgccggggga gcggacggtg ccggtcgagc cgctgccgct ggcctccgac gccgagcggc
     1501 tgttccggga ccgggccacc gcggccgacc ccggcttcac cgctggccag gccgttgtcg
     1561 tcgaactctg cggcaggctg gacggcctgc ccttggcgat cgagctcgcg gccgcacgca
     1621 gcgcgtcact cggccccgac ggtctactgg ccgcgctgga cgacagtctg ctgatgctgg
     1681 ccggtggtcg tggaccggtc gagcggcacc ggtcgttgcg cgccgtgctc gactggagtc
     1741 acgacctgct cgcggcggac gaacgagagc tgttccggcg cctgtcggtc ttcgccagcg
     1801 ggtttgacct gacggccgtg gccgccgtcg tgcgcgggcg gacccgcggc gcggtcgccg
     1861 acgtgctcgg ccgactggtc gacaagagcc tcgtcgtgca ccaaggaggc gcacgcagca
     1921 ggtggcggct gctcgagacc gtgcggacgt tcgccgcgga gcaactcgac gccagcggtg
     1981 agcgagccga ggtccggcag cgacacctgc actgggctgc ggcggccgcc gcggagttgg
     2041 agacccggct ggacggcgag tggcggggtg actttgacgc cgtcgtcgac gacctgcgcg
     2101 gcgctgtcgc cgccgctcct ggtcaagacg tcgtcccgca ccagctggcc agatcgctgg
     2161 gccacctgtc ctatgcccgc cggttcctgc acgaggctcc cgaccactac gaggaagccg
     2221 ccaggcgggc gctcacgcca aagcaggccg ccgaggacct gcggagcgcc gccgatggcg
     2281 cgcacgcctt catcgacacg ggccgagcct tccagctgtt gctcgcctcg gccgagcagg
     2341 ccagggcggc cggggacggc aataccgaag cgatcgcgct cgccttcgcc gtcaccacag
     2401 ccaaccggca cccggccggg ttcacggtgg aggtgccgca cgagcggcta cgcgccctgc
     2461 tcgatcgggc cacggccatc ggtgaccacc aggaccggtc cgtcgccgcc catctcgccg
     2521 cggcgaacgc gtggaacgcc aacggggaga aggtcacgcc cgaacccgcc ctggtgcccg
     2581 ccgcggtggc caccgcgcga gcgaccggcg atccggtgct gatcagcgcg gggctcgacg
     2641 gggtcggcat cgcggcactg gccgctggcc ggttccgcga ggcgcaccgc gttgccactg
     2701 agcggatgtc cctgctcgac gacatgtccc gcgacgaccc gtacccggca cccgagatca
     2761 ccgacacgta ccacatggcc gccacctgcg cggtcgccgt cggcgacctg cgcaccgcgc
     2821 tgtccgtggc caggctggcc gctgccgacg acctcgtcgg cagccagtcg gccgtcgcca
     2881 tgagcgccct gctgcctgcg ctggtgctgt cgggcgacct cgacgaggcc gtgcgccacg
     2941 ggccggtcat ctgggacgtc tgtgtccgcg cggacagtcc gctcgcgtgg gtctcaccgg
     3001 cggtcgcgtc ggtcgcgctg gcgcacggcc tgctcgacga cgaggacggc tttctgctgt
     3061 ggcgagccag ggccgagcag gtcgctggcg atgcccaggc gaggtacctg gcgtcgtacg
     3121 cggcgttcgt cgacgcgcgg gtggccctcc acaccggaca gacggacaac gccgacgtgc
     3181 tgacggagcg tgccttggtc gatttcccgc gccaggactg gcaccgcgcg ttcgcccacg
     3241 ccgccggagc ggagctggct gtcgcgagtg gactgccgga cgcggccgac cgactggcgg
     3301 cggcatcagc cacggccacc gagaacgact gggccaccac ctgcctcacc cgagctcgcg
     3361 gtcggctcga ccacgacgcg gacgcactca ccacctccct cgcaggctgg gaccgtatcg
     3421 gcgcccgctt cgaacacgcc tacaccctgc ggctgagcca ggaaccgtga ccggcggacg
     3481 ccctcactgg tgcggtgtcc ccggcgaacg ttcgtggaca ccgcaccagc agccgtggaa
     3541 ctgccggaca gcgtgcaggg cggtgacggc gatcagccga gtcgccacct catgccaccg
     3601 gtcgtcgcgg aatcaggaca gcgcatggaa ccggagcgcg gaccggaaac ggtgtgcgag
     3661 caggaccgcg gtcgcggcga agcccacaca cagtcccgtc cacacgcccc agccgcccca
     3721 cccgaacgtg taggcacaca ccgccatgac cggaacgccg acagcccagt aaccgatgag
     3781 cgtgcaccgc aggccggaaa cggtgtcccc gagaccgcga agcagaccga ccaggatgtt
     3841 ctgggtgcct ttgctgtact gctgtgcgac ggcgaacagc agcaggatgg aggcggtgcc
     3901 gagcacggcc gtgtcggtct cgtcgtgcag gaacggccac aacatgccta acggtgccag
     3961 cacgtaaccc aggctcacca ccgccatgaa cgtccacgac agcgtcaggg cgcgccgcgc
     4021 gatcgtccgc gcatgctggg gttccgactg cccgaccgcg cggctgacca gtatcgacga
     4081 gccttgcgac aggccgatgt tgcactggta cacgatgtag gcgagctggt tggcgacgtt
     4141 ggacgcggcg agcatcgccg ggccgaacac acccatcaac agtgtcgcta tggacgtcat
     4201 cgcggcctcc gacccgtagg tgaagcagat cggtgtcccg tgccgggcga tccgccggac
     4261 caccgccgga tcggcccgcc acgcgctgag cgacaccatg gttcgcagtt gcgggtcccg
     4321 gcggatggtg gtggcgaacg ccgccagagt gaagaactgg accactgtcg tcgacaggcc
     4381 cacaccggcg acgccgagcc ggggcaggcc cgcccagccg tagatgaacg ccgcgttgca
     4441 gcccgcgttc accgcgatcg acaccagcgt cacggccagc agcgaaccgg gacggcgcat
     4501 cccgaccgcg aactgccgca acacgttcag ccacaccatc gggaacagcc cgcccgccag
     4561 cgcgaacatc atcccgcggg cgagtgcggc gatctcgggg ccctgaccga gcagcggcag
     4621 ggccagtgcg agtccacaca ggaccgcggc acccgctgcc gcggccaacg tggccaccag
     4681 gagcgccgat cgcagcagcg accggatctc gtcgcgcgcc tggtcgtcga gcgtgtctgt
     4741 gccggtgcgc cgctcacccg ctccggcagc ggtggccacc aggttcccga cgccggtgac
     4801 cataccgacg cacatggtgc ggacctggtt gtacaacagg agcgccagac cacccgccgc
     4861 caccgcggac acgcccagca gcccgagcat ggcgaggtcg acgctggtga gcgccacctg
     4921 cgcgagttgg atccccgcga tcggcgcggc cagcgcgacg agcgcggggt atccgcccgc
     4981 tggtgttgtc accgagcgtc tcacgcactc accgccacgg gcaccgccac ccgctcgggc
     5041 acggaccgcg ccatgcgggc cgcgatccgc accgacacgg cgtaggtggt gaaccaccgt
     5101 tccacccgcc gttgggcgag ttcgtcgagc accgcgcggt cgcgcaacca gccgtcgtcg
     5161 tacaccgagt ccaggtattt ctctccggcg tcgttgcaca acaccacgac agtgctcccg
     5221 ggtgggtacg cgaacaaccg ccgcaacgcc acgtggatcg cgccgccggt cgtcccgccc
     5281 accaacaagc cggtgcgctg tgcgaccacg cgggcgcccg cgaacgcgtc ggcatcggag
     5341 acccggtggg catcgtcgat cacggtacgg tccacattgg agccgatcgg gaagcccgcc
     5401 gggctgcccg cgccggtctg gtggtagatc cccggcgggc cgccgaagat gatcgaaccg
     5461 accggctcga ccgccaccga ccgggtccgt gcgcccgccg cccgcaaccc gcggatcgtg
     5521 ccgcacagcg acccgcctgt gccgaccgcc gcgaccagca cgtcgacggt gcccaactgc
     5581 cgcagcagtt ccgacgccaa cccgccgtag ccgttggcgt taccgggatt gttgtgctga
     5641 tcgggatggt aggcgcccag ttcggcggcg agccgcgccg cgacctgccg tctgcgcacg
     5701 gaactgggac cgccgtccga cgggcagtcg acgaacacca gccgcgcgcc catggcggtg
     5761 agcatgcgca gtttctcgcg ggccgcgtgc tggtcgacga cggcggtgaa ccggtagccg
     5821 cgttccaggg cgaccaacgc cagcccgcac ccggtgttgc cggatgtggg ctccacgatg
     5881 tgaccacccg ggcgcagctt gccgtcccgc tccgcctcga tgatcatctg ccgggccatt
     5941 cgcaccttgc acgagccggt ggggttcatc tgctccagct tgagcagcag gcgggtcccg
     6001 gtcccggtgt gcgcgagctc gagcagcgga gtgtccccga tcaggtccga ggcacgctgg
     6061 acgatggtca tgggatctcc tggatcgggt agtcggtgtc gacatgccag cggtcaccgt
     6121 cgacgaccac cttcggcggc aacggcaact ggtggaaacc gctctcgttc ttgtccatct
     6181 ggtagccagc cgtgttgggg tacacgagca ggtcgcccgg caccgggcgg gcgggcagtt
     6241 ccaccgtgcg ccaggtcagc acgtcgtact ccaggcaact ggaccccgcg acggcggtcc
     6301 ggaccggcgt gtcgccctgc ctcggtccgc cggtccggac cagtatcggg tcgggcagga
     6361 actcgctgcc cttccattgt tcggacaggc tcatgctcag cccggcgacg gtcgtgacca
     6421 ggtggccggt gctgtccttg ctgccttgta ccgggaacac cgagaacccg gcgctgtcca
     6481 gcagcgcccg gcccggttcg agcagcaggt cgatcccggc gtcgcgcagc cgggcggcca
     6541 gcggtgcgcc gccgacgtcg tgccgcagga tctcggtcac catcgccgcg ccggtggggg
     6601 actggtggta cgggtaggtg cgctgcggat cgcggccacc gtggaaccag ctgtcgtgct
     6661 tgctgtcggt gaaccggcgc cagtcctctt cggacacgta ggccacagcg atcccaccgc
     6721 cgatgttgat cttcgaggcg ggatggccga gcgtgcgggc ccgtcggcac agctcgatca
     6781 ggtgcgcggc gagctcggcg cgcggagccg ggtcgtatcc gtcgagatgg aacgagaaac
     6841 cgcgcagtgc gaccatcgtg ccgagtgtgg cgcaccggtc gacagcggcc gtgagatcgt
     6901 cgggagcgaa gccgaaccgg ctcgtcggcg cggtcggcgg ccggacccgc aacagcacgt
     6961 cggcggtcac ctcgagacgg cgggcgtgga cggccacgcg ctccagttcg tcggccgagt
     7021 cgaccgccag cagacagtgg tgccgcagcc ccagccacag cagaccgtcg gacttggcgg
     7081 caccggtcac cccgatcgcc tcaccacgga caccattgcc caaggcgtgc accagctccg
     7141 gcacgctcgc cacgtcgaca ccggtgccgg ggcgggtgca ttcgcgcagc cacgccgacg
     7201 ccttgttggc cttcttcccg tagtacacga caccacgggt gttctcggca gtcagtgcgt
     7261 cgaccagacc ggtgaggttc tcggcgaagg tcggcggatg gatcacgtgg aacggaccgc
     7321 cgccgacggc ccgagcgatc tcccacagca tcgagctggc gcgcagccga cgtgcccacg
     7381 gacgctccag tgcgggcagt ggtggcgaca gtgcggtggt cacgaccaca gggtcacctc
     7441 gcgtccacgg cccgccgcga tggcacgcgt ggccagcgcg tgcgccaccg ggatgtcggt
     7501 gatgatcatg ccgctgctga acgcgaacac ccggtcgtcg tccgtgcggc gtcccggggc
     7561 ccggcccgcg aggatgtcgg gcagttcggc gtcgatgcgg aacacgccgt ccgggcccgc
     7621 catccgccgt ccggtgacct ccacctgggc ggcactggtg gccaccgtgt agtcggcctg
     7681 cgcgagcgtg ccttcctgaa cgcccttgct ggcaacggag atcagcaaac cgcccggcgg
     7741 cagccagccc agccggatct tcgggtgcgc cgcccggccg gacgccgcca ccacgatgtc
     7801 cgagtcggcg gccgcggctt ccacgtcgtc gaccagttcc acctcgcggt ccgggaactg
     7861 gtcggtcagc gccgcgatac tggcgcggat accgtcgggg tgggtgccgt acagccgcag
     7921 ccgctccagc cccggcagcg cggtcagcag atagggcaac gtccggattc cctgtgcgcc
     7981 ggtgccgacc atcaacgccg agcgcgcccc cggcttggcg caagtccgcg cgatcagcgc
     8041 ggtactggcg ggtgtgcgcg tggcgccgac gcggtggcag tccagcagcg cgaacggagc
     8101 accggtggtg tcgtcgtaca gggtgatggt cgtgtagtac ctctcggcag aggtgctgcc
     8161 ttgccggtaa ctcgtcttga acgcagcctg ctcgagcgag ccgtcgtagc cgagcatcga
     8221 gtacgcgacc gagtcccgcg tggggtcggg catcggcacc atcagcttgg ccggattctg
     8281 cgaccggccg gacgcgaaag ccaggtaacc gtcctcgacc atcgtgatcg cctcgtgtgg
     8341 ggcgagttcg aggtcgtgca ggtccttacg ggtgaggatg tgcagtgcag ggccgtaatt
     8401 cacgggattg acaacctttc tggcgcaggg aattcgtctc ggcaccgtga aaccgtcgac
     8461 ttcgcgtggt tttgggcgtg cgggaacagg ggcgcacgcg tacgcggacg tccttgggaa
     8521 acgaacgtgt ttctacgtga gaatgccgag tcgaatgacc gatcaggtca gtgcagggaa
     8581 tcgagaagga acacgcgcaa ccgtcatcag gggtggctgt gaatgattgc ggagccgcag
     8641 cagggcggtc aggtccgcgc gacgcagacc gtcagcagga acgcgcgtcc gtgccccgcc
     8701 ctgatcggcg ggggatgctt tcgcctgccc ggctgccggt agcccccgtg cacgcgatcg
     8761 ttcagcggca acgggaacac gaacaggagc agcgtgagca ggagggcacc cgagtcgtcc
     8821 tcggctcgtg tcaggacatc cgcgccatcg ggactgtgca ccaacgcgcc gcgtgtctcg
     8881 ccgtccactt cgccgatgtc ggcggaaacc ggaccgccgg tcgggccgaa agacagggaa
     8941 gcggcggaga ctcccggatg caggacatga gctccgtggt ccccggcgca caccgtcaga
     9001 tacagggagg ccaatgcgcc gagggcgaga agtattgccc gcacccgccg gtagcggatt
     9061 ctgggggcag gcaaaatgac tcgcacagac cccgaagcta ctcagcgggc cggttcggtc
     9121 gataccgcca gacgggccat ggtgttcgcg gatttacccg gcgcttcgac gaactttcag
     9181 gttatgggga ggatttatga gtggagttgt gtgagcaaac tagaatccgg atgttcatcg
     9241 gtcatccggt gagaccgccg gtgaccatga ggcggggtgt ttgtgagtgg tttggtgctg
     9301 gtgcgtggaa caggtgggga tgtaggaaga ccacgtctcc tggttgacct gtcagttcca
     9361 ccacacgaag atcgacgccg tcgacggttg ttcctccgtg cttcatcagc gtggcaacgc
     9421 gttggtccgc ggcacccggc tcccggatgc tgcgcagcca cgggtcggcg ctgaagatcg
     9481 cgttacgtgc cacagatgag cgtcccgggt cggccgggtc gattcggtgc aggtaccgct
     9541 cgacgaggtg gtgggatccg gacaggacga cagtaccccc gccgcccgcg gcgaccggcg
     9601 cgagaaaggc cagccatttc aacctcagtg ctgatcgggc tgggaagtcg atgtgccagc
     9661 catcggctgg tacgttccac tgtccatggc tggggaaggt gaccagtggg ccgccccagc
     9721 tgaacggctg tttccagctc tcctcgccga gcagttcgct gatcgccgcg acgatcgccg
     9781 gggtggccag cgcgtcgaat gttctctttc gccgcagtga accgagtttc ctcggctcgg
     9841 tcactgtcca tgtggaacgg tcggtgcggg agaggccatg tgtccgcgtc agctccgacc
     9901 agaccgcgtc agccatcgcc gtggcgagtt caggtgggaa gctctggtcg agccggacga
     9961 agccgtgttc ggcgaaacgc aaccggagat ccatgtcgct ccttgagtgg ggaccctcaa
    10021 gcgcggcacg ggacaggccc gcatgcaggc agccgccaac ccatgccgac ccgacggtcg
    10081 gtcatgggcg ggcacctcag cacgcccaac accgcgacca taacagccgc tggctcggca
    10141 gtcatgtcat ttgcccctgg ccaagccaga tcgtcgaccc cgtctgtcat gagccagcag
    10201 atcattctgg ttcgtcccaa gtcggttgtc acagaacgac gttggacacc ctttgtacgt
    10261 ttgtggacac tcgcggacca ggagagtggt cagactgggt ggtggtcagg gccagcgttg
    10321 agttcgatgt cgtgctccag cagggccttc atcgcacaca aggcggtgct gaagccgccg
    10381 acagtgtcgt tgagccagcg gacagcgtcg tcccctgaac cttggaagcc ggactcggtg
    10441 acctccacga acgtggcatc ttccgcgtgc ggggtgaacc gcagctcgac cgtggtcggc
    10501 tgctggtagt tcccccactc gaaccggatc agacggcctt cctgcacttc cttgacccgc
    10561 acctcgacgg atgcgccata cgtctcccac tcccagcgca gttcggcccc cgcgaccatt
    10621 gggccgctgc tcttgctgta ccagaaacgg gaagtgacgg ccggatcagt gaacgcctgg
    10681 aacgccacct ccgcgggcct acggacgatc atgcatgcat acgcgacggg agcctgcgtg
    10741 aggtgaagtt tctccatgga cacggtcatg ctctcctaaa agtccctcac gggttcggac
    10801 cagagttcag aaatgtgcgg caaggtcgat cacctggtcg agcagcgtcg gaacgtcgtg
    10861 atgtcggcca tcagcacgtc ccgccccctc taccttgagc tgtcaaggca cgctaccgag
    10921 gataatcttg aggtgtcaag gtaaggtgtc gatcgtgacc gcctcacgag tgcgaaggcc
    10981 ccagcgggca tgctcgacga gttcctggcc ggtggaaagc ggctgagcgt caggagacgt
    11041 acacggtcgc cagcgtgacg ttggcgccac ggaggcggat cgtgacggtg ccggggtgtg
    11101 tcggggtgaa gtcgatgtcc tccggctcgg tggagtccac atcggcgttg ctgtccgggt
    11161 atccgtcgac agtgatgtcg tcggtccggt ccgcccggat gttgatcttc accggctggc
    11221 cgaccgccac tcgcaggacg gtgtcgggtg gctggacggc gcctgcctgg taggccacgt
    11281 cggcgactgt cttggtgacg atcggcccgg tcgacgaggg catcggctgg gaggacacgg
    11341 tcgccggtgg gctgttgggc ggattcgttg gggatatcgg cttgtccgat gagcagccgg
    11401 cgctcaccac tgtcgcagcg atcacgccgc tggccaccat gccagccgcc agccgccgca
    11461 caagcaccgg gtacgtggtc gacctggtca ttgagtcaga ttaagactga gtgcggccaa
    11521 ccgcagagcg ggtaacccga acgggtcgct ggcgatcatg tgtgcgcgag cgactcaccg
    11581 acgccgcgcc ggaggccagc gttgcgttac ggagaaggac gtcgagttcg tacgggcgga
    11641 tggatccggt gacccggatt cgtcgcccgc tacgaacggg cggtccgtga aacctcctag
    11701 ggtcgccagt gaacgaggcg agcctaggag gcggagcatg cggacactct tgcgtggcgg
    11761 tcgtgtcatc gacccggcga cgggttttga cggtacggcc gacgtgctgg tatccggcgg
    11821 ggtggtcacc gccgtcgggc cgagtctgcc ctcgcagccg ggtgacacgg agatcgacgt
    11881 ctccggtctt gtcgtcggcc ccggtttcat cgacctgcac agtcacgtgc acaccatcgc
    11941 gggccagcgg ctgcaggcga tggacggcgt cacgaccgcg ctcgacctcg aagccgggct
    12001 gatgccgatc gagcgggcct acgccgaggc cgccgctgcg ggaaggccgc tgcactacgg
    12061 tttctccgcg tcgtgggggt ccgccagggc gcacgtgctc gccgggatcg agcccgacgc
    12121 caagatcgac agcggcctcg cggtgctcgg gaacccggcc tggcaacgga cttcgtcgcc
    12181 gaccgaactc aacgcttggc tgtcccttct ggagggtgag ctcgctgcgg gcgcgctcgg
    12241 gatcggggtg ctgctcggtt acgctcctct gacggatccc ggcgagttca tggctctcgc
    12301 ccggctcgcc aaacaggcgg gggcacccac ctacacccac gtccgggaac tggtcgaagt
    12361 ggatcccgcg acccctgtcg acggctcgga ggagatcgcg atcgtcgcgg ccgagaccgg
    12421 cgccgccatg caccactgcc acgtcaacag cacttccggc caccagattg accgtgtgct
    12481 cgccgcgctg gaggcgagcc gctcggccgg gtcgagggtg accgtggagg cctatcccta
    12541 cggtgcgggc agtacgggga tcggcgcggc cttcctctcg ccggagcgtc tgaagatgaa
    12601 ggggctctcg ccgtccagcg tgatcatgct ggagtcgggg gagcggatcg ccgacgaggg
    12661 gcgtctgctc caggtgcgtg ccgagcaacc gggtgcgccg tgcatcctgg agttcctcga
    12721 cgagcgcagc ccgcgtgacc tcgcgctgct gcgccaggcg ctcgcgttcc ccgactccat
    12781 cgtcgccagt gacgccctgc ctgtgtactg gaagaacggc accagcgaga gcaccgaatg
    12841 gccgttgccg cccggtggcg cgacgcatcc gcgtacgtcc gggacgtacg ccaagacgtt
    12901 gcgcctgatg gtgcgcgaga gcgaggcctg gacttggctg gaggcgttcc ggcgctgttc
    12961 ctacctgcct gcgcgcgtcc tcgacgaggt cgcccccgcc gcgcgggcca agggcaggct
    13021 cgacgtcgga gcggacgccg acatcgtcgt cctcgacccg gacaccgtca ccgacgcggc
    13081 cacctacttc gactcgacaa ggccttcggt cggcatccgg catcttttcg tttcgggcgt
    13141 tgctgttgtc accgacggaa acctgcgcac cgacgcgttc cccggcaagc cgctgcgcgg
    13201 tgagccgcga tgagcgaact gctcgccgac atcgagacgc tcgttcgctg cgagtccccg
    13261 tcgtcggatc acgccgcggt ggcccgaagt gccgaggtcg tggcggggtt gggccggaaa
    13321 ctgctgggga cggagccgga gcggatcgtc gtcgacggcg tcacccacgt gcgatggcga
    13381 ttcggcggcg gaccgcctcg cgt
//
