LOCUS       NZ_CP009163            10852 bp    DNA     circular CON 03-FEB-2024
DEFINITION  Burkholderia pseudomallei K42 chromosome 2, complete sequence.
ACCESSION   NZ_CP009163
VERSION     NZ_CP009163.1
KEYWORDS    RefSeq.
SOURCE      Burkholderia pseudomallei K42
  ORGANISM  Burkholderia pseudomallei K42
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Burkholderia; pseudomallei group.
REFERENCE   1  (bases 1 to 3327069)
  AUTHORS   Johnson,S.L., Baker,A.L., Chain,P.S., Currie,B.J., Daligault,H.E.,
            Davenport,K.W., Davis,C.B., Inglis,T.J., Kaestli,M., Koren,S.,
            Mayo,M., Merritt,A.J., Price,E.P., Sarovich,D.S., Warner,J. and
            Rosovitz,M.J.
  TITLE     Whole-Genome Sequences of 80 Environmental and Clinical Isolates of
            Burkholderia pseudomallei
  JOURNAL   Genome Announc 3 (1), e01282-14 (2015)
   PUBMED   25676747
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327069)
  AUTHORS   Baker,A.L., Bunnell,A., Chain,P.S., Chertkov,O., Daligault,H.E.,
            Davenport,K.W., Davis,C., Gleasner,C.D., Johnson,S.L., Koren,S.,
            Kunde,Y.A., Mayo,M., McMurry,K.K., Robison,R., Rosovitz,M.J. and
            Warner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2014) Bioscience, Los Alamos National Laboratory,
            P.O. Box 1663, MS M888, Los Alamos, NM 87545, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-AUG-2013
            Assembly Method :: Newbler v. 2.6; Velvet v. 1.2.08; Allpaths v.
            44837; Phrap v. SPS - 4.24; Hgap v. 1.4
            Genome Coverage                   :: 415x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/03/2024 03:30:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,503
            CDSs (total)                      :: 6,427
            Genes (coding)                    :: 6,257
            CDSs (with protein)               :: 6,257
            Genes (RNA)                       :: 76
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 99 of 170
            Pseudo Genes (incomplete)         :: 105 of 170
            Pseudo Genes (internal stop)      :: 19 of 170
            Pseudo Genes (multiple problems)  :: 47 of 170
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:06:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1466016
            Orig. end                         :: 1476868
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP009163.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            The DNA was obtained from Dr. Richard Robison, Brigham Young
            University (BYU), richard_robison@byu.edu.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10852
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10852
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10852
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5852](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5852)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            448..1068
                     /locus_tag="X993_RS23765"
                     /old_locus_tag="X993_4605"
     CDS             448..1068
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894570.1"
                     /locus_tag="X993_RS23765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="X993_4605"
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_004194492.1"
                     /transl_table=11
                     /translation="MNWIAFSHGAMLCASLIVTIGAQNAFILRQGIMRSHVGKIVLLCT
                     LSDMLLIGAGVGGASVLVERYPAFVHAVLYVGLAYLAWFGLSALRRAFKPGHETLDATG
                     AVAAPPAQRAWPIVLMTLAFTWLNPHVYLDTFLLIGTAGAREPEGARGAFAIGAMAVSA
                     VWFVALGYGARLLAPWFKRALAWRVLDGAIGGMVLFLAAVQLR"
     gene            complement(1240..2229)
                     /locus_tag="X993_RS23770"
                     /old_locus_tag="X993_4606"
     CDS             complement(1240..2229)
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852411.1"
                     /locus_tag="X993_RS23770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="X993_4606"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_004532676.1"
                     /transl_table=11
                     /translation="MARRKLERWSALDLAEGLQLAHAVAALQTLGVVDAMAEPTTAQTL
                     SAACDLDPELLRGVLEYAASRTNLIRKTGDRFSASEHYTAQARFLLNLYAGAYADNASR
                     LATLLRRPALAGTTVDLVAHARAFEASEAGGGALAAIISQLHLNHVLDLGCGAGTLLHA
                     LAADDREFVGWGLDRNPSMCKAARMRARQAGIAARVKVFQGDGRHPGASIPPRVLARVR
                     NVVASQFVNEMFRGGTSRAQAWLRRMRRLLPDRLLVIADYYGRLGHGFQTPHRETLLHD
                     YAQLISGQGVPPPNADAWLAMYRAAGCRPLHVIEDRAATTRFVHLVAL"
     gene            complement(2243..4186)
                     /locus_tag="X993_RS23775"
                     /old_locus_tag="X993_4607"
     CDS             complement(2243..4186)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004549365.1"
                     /locus_tag="X993_RS23775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="X993_4607"
                     /product="radical SAM protein"
                     /protein_id="WP_044491338.1"
                     /sec_met_domain="PF04055 (E-value: 1.2e-14, bitscore: 52.4,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLLLVDNLVMPEEGSLAYLDVHPHLGLLALAAVAEADGHRVRIY
                     DPKRLIRDGTLAYDATLYERASRAILAERPDAVGFTALGCSFLFALNVAALLKRREPDL
                     PVLLGGPHATMLHRQILERFPQFDIIVRYEADEILPAVLDCLEQRTFDVIPGLSWRATG
                     CGAPLRFTDGKPKVEELDRLPIASYDHYPVDDLGLSMLRIEAGRGCPFACTFCSTAGFF
                     QRSFRLKSAERLVHELDILHRRYRVSDFKLDHDMFTVNRHKVMAFCEAVAGRGYRWRAS
                     ARIDCVDEALLRKMADAGCVNLYFGVETGSERMQTICRKRLDLRLVEPILAAADSFGIE
                     TTASFITGYPQESGQDHDDTLDMIGRCARRPSCLTQLHMLAPEPGTPLFDEHGAQIAYD
                     GYGGPYNTRLLGPEDEREVLDHPDIFQTYYYYPAALPRARHIFAVEAVDVLRRAGPIIL
                     AYALREFGGRLSRLISAWRRFADETSPGALPDAAGLEAFVAARFGRGHHLHSLFRYALR
                     LSAARAPADVVAEGPYEPDRPCMLAENVGLLEDIHNCEALVDRLRRSNDGAPALGDADV
                     GGLGAYIVRADGEAVTGYWVESGIAAILDLFRAPRSCRAAAQWLAHVTRNDGVDASIFE
                     PLLRRGILVPAR"
     gene            complement(4183..5004)
                     /locus_tag="X993_RS23780"
                     /old_locus_tag="X993_4608"
     CDS             complement(4183..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543057.1"
                     /locus_tag="X993_RS23780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="X993_4608"
                     /product="hypothetical protein"
                     /protein_id="WP_044490939.1"
                     /transl_table=11
                     /translation="MNARTIHALIAASAKDPQLIEHWRRHPDLLRGAGIAPGDLDWRAL
                     EHFVGLSLKVRHNGLRGDFPLSFRLLSVVGLEIEVFAAYASDCAESGHVFAATPQERAR
                     DLIAFLDRWLDPAQRNHVLLWDILRHEYALACLRQAGDAPLPDARHRARATAGGIPTLN
                     GSVVQHVMRCNPDDLAAALHQKEPPLDAVPAVDTYCCYWRSAETGEIRLLRVDTLGYFA
                     IEAIDGRRSAADICHRLLGNRRLLPQFLSSLDQLAGIGIVRFARPLGCAAA"
     gene            complement(5001..5852)
                     /locus_tag="X993_RS23785"
                     /old_locus_tag="X993_4609"
     CDS             complement(5001..5852)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603954.1"
                     /locus_tag="X993_RS23785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="X993_4609"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_038725437.1"
                     /sec_met_domain="DUF692 (E-value: 9.1e-35, bitscore: 117.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAFDRDAGAPRIGLAYGPGMAEFAARHAHLVDYIEVPFELLRFS
                     PTIAELQQTIPFVLHCASLSVAGFVPPDDSTVDAIERTAVRTGTPWIGEHLAYISADPI
                     GEALGGTHEPTSLSYTLCPQLSDETVRRVADNLAVLAPRFPVPLIVENSPQYFSIPGST
                     MGMTDFIRAIADRCDVGLLLDLSHFLITAYNTGADVHREFARLPLERVVEVHLSGMSIQ
                     SGAAWDDHSLPASPILFELLERLLAVARPRALTFEYNWSPYFPLSVLTSHIERARALMG
                     LV"
     gene            complement(6026..6226)
                     /locus_tag="X993_RS23790"
                     /old_locus_tag="X993_4610"
     CDS             complement(6026..6226)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525608.1"
                     /locus_tag="X993_RS23790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="X993_4610"
                     /product="hypothetical protein"
                     /protein_id="WP_004201048.1"
                     /transl_table=11
                     /translation="MATKPKKTKGVSVSIEGKLPKMTLDMPVDAKKIKAIQKCLENGKL
                     TITMSKVDLAGGRMGEGYLYD"
     misc_feature    6203..6212
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.0"
                     /tool="antismash"
     misc_feature    complement(6307..6321)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.99"
                     /tool="antismash"
     gene            complement(6376..7782)
                     /locus_tag="X993_RS23795"
                     /old_locus_tag="X993_4611"
     CDS             complement(6376..7782)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009981062.1"
                     /locus_tag="X993_RS23795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="X993_4611"
                     /product="cytochrome P450"
                     /protein_id="WP_038754080.1"
                     /sec_met_domain="p450 (E-value: 2.6e-35, bitscore: 119.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGLPLSLPRVDSTDSLFADPLAFLARARAQCGDVFVLREHGPIF
                     SRASDCSGVIAVFGEHRLRQVLTETDTFALPMSAAAKMALPKNLVNLNRGLHSMREPEH
                     GRHKRLLTGTANRALFDAHRSGMQTDLSRFCEMLNGDGRISVVSRMRELTAKLASRLFL
                     GPQCEKDAELTFLLSAYFTLRREASSPNAHDPLQYRDALIGAGRQLDRALRARIRRYRK
                     APAGECAGLLQRLATAGQPGSPALSEDEIVGHANVMFVSSTEPVAISLAWLLLVLSQLP
                     DLRRALRAESAARASSPASPYDAPLLERVINETLRLLTPNALMVRATTQAVSLQGVALP
                     ARCEIVVCPFLVHREANAFAHPHAFSPSRWETARPSPYEYFPFGAGGHFCAGRNLALSL
                     IGEVLSTLLSRFDFVLDIEQFIDWRIHIMLMPKGDPTLTAHPVDERRDTPSPKWRGPVA
                     ELFHFAPGLS"
     gene            8196..8912
                     /locus_tag="X993_RS23800"
                     /old_locus_tag="X993_4613"
     CDS             8196..8912
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548999.1"
                     /locus_tag="X993_RS23800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="X993_4613"
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_038760625.1"
                     /transl_table=11
                     /translation="MRHAHRFSRRECGMSDSFGHSPFEMFRQMFGGHRRGGGRHGFGPF
                     GGFGGFGGGGPGGFGGFGDDAMPRGRQFSADDLQLLLLALLAKQPSHGYELIKALDTRS
                     SGFYAPSPGMVYPALTYLEELGYVTVQAEGNRKRYALADAGRAHLDAQRERVEMLFAKL
                     AHLGRKMEFMRRAFAGEAPAAPEDDTRGWLPEFVEARMALKQALFRRSGANADEQRRIA
                     AILRRAVADIEGRAQG"
     gene            8952..9779
                     /locus_tag="X993_RS23805"
                     /old_locus_tag="X993_4614"
     CDS             8952..9779
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /GO_process="GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1200: vibriobactin
                     utilization protein ViuB"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532447.1"
                     /locus_tag="X993_RS23805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="X993_4614"
                     /product="siderophore-interacting protein"
                     /protein_id="WP_004194445.1"
                     /transl_table=11
                     /translation="MTQPTTERAVVRVRHTLKFRLLQVKRVVDVTPHLKRVTLAGDELD
                     DFESASFDDHVKVFFPPPGEDAPARPEFGPNGIVFPQDRPRPVARDFTPRRYDRAAREL
                     DLDFVLHHPGPASQWAAQARVGQTLGIGGPRGSFVIPKDFDWHLLIGDDTALPAVARRL
                     EELPAGTRVAAVLEVADPSAQIAFETDANLYVVWRHRGDAASGERGASRLVDALRELPL
                     PAGEGYVWAAGEAAAMRGVREHLCRERGVAKSRIRASAYWKHGAIAVHESLED"
     gene            complement(10103..10351)
                     /locus_tag="X993_RS43440"
     CDS             complement(10103..10351)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194583.1"
                     /locus_tag="X993_RS43440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_011852397.1"
                     /transl_table=11
                     /translation="MWRRADARASRDASGRWNLNARAGRRRCGVDRRENGGEANETALS
                     ARGMAAKACDAMGQRGAAALERRGGAVRSGANQSRLP"
     gene            10545..10781
                     /locus_tag="X993_RS23810"
                     /old_locus_tag="X993_4615"
     CDS             10545..10781
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196519.1"
                     /locus_tag="X993_RS23810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="X993_4615"
                     /product="hypothetical protein"
                     /protein_id="WP_004196519.1"
                     /transl_table=11
                     /translation="MTRHHTHAGIPRTLKIVGYCGLAAIALALFVAMCAILKDSALDRE
                     AARQLQNGNVELHGGADVEPGSTNDAAQARTPG"
ORIGIN
        1 caaccgcgtc gacgaaccag gtcgcgacgc tgtcgtcgtt gacggccacg cgcagcgtcg
       61 gccacgccgc cgcttgcgcg cccgcgaacg cgggcatgtg cccggacagc tcggcctcca
      121 gcatccgcac gcgctcggtg tggcggcaca gcagcgcgcc cgaggtcgtc gcgacgcacg
      181 gctgcccgcg cttcacgagc acgctgccca cgcgctcctc caagagcttc acgcgctgcg
      241 acaccgccga aggcgacacg ttgagcgctt tcgccgcccg ctcgaacgag ccgtggcgga
      301 tcacttcggc aagcgcatcg agcagtgcat agtcgagcat cagttttcct taatcggcat
      361 tagatcattt agcttttctt actcaagcga tcatcgcaga ctagcgcctc ttgccccgtt
      421 agtccaccgc cggaagccgc aacgaacatg aactggatcg ctttttctca cggcgcgatg
      481 ctctgcgcat cgctcatcgt caccatcggc gcgcagaatg cgttcatcct ccggcagggc
      541 atcatgcgtt cgcacgtcgg caagatcgtc ctgctctgca cgctgtcgga catgctgttg
      601 atcggcgcgg gcgtcggcgg cgcatcggtg ctcgtcgagc gctatccggc cttcgttcat
      661 gccgtgctgt atgtcggcct cgcctatctc gcatggttcg gcctgagcgc gctgcgccgc
      721 gcgttcaagc ccggccacga aacgctcgat gcgacgggcg cggttgccgc gccgcccgcg
      781 cagcgcgcct ggccgatcgt gctgatgacg ctcgcgttca catggctcaa cccgcacgtc
      841 tatctggaca cgttcctgct gatcggcacg gccggcgcgc gcgagccgga aggcgcgcgc
      901 ggcgcgttcg cgatcggcgc gatggcggtc agcgcggtat ggttcgtcgc gctcggatac
      961 ggtgcgcgat tgctcgcgcc atggttcaag cgcgcgctcg cgtggcgcgt gctcgacggc
     1021 gcgatcggcg gcatggtgct gtttctcgcg gcggtgcagt tgcgctaacg cggcggatgc
     1081 gcgttggcgg tgccgacgcc atctgaagcg cggacggcgg cacgcggcgc acggcggcga
     1141 gcgagccacg aacgtcatgg ccgcacgccc tgagggaatc ggccagccgg cgcacgcggt
     1201 cggcgcaagc ggcgccgccg agccgcgccc gtcatgccgc tacagcgcta ccaggtggac
     1261 gaagcgcgtc gtggccgcgc ggtcttcgat cacatgcaga ggccggcaac cggccgcccg
     1321 atacatggcg agccacgcgt cggcattggg cggcggcacg ccttgccccg atatgagctg
     1381 cgcatagtcg tgcagcagcg tctcgcggtg cggcgtttga aaaccgtggc cgaggcggcc
     1441 gtagtagtcc gcgatcacga gcaggcggtc gggcagcagg cggcgcatgc gtcgaagcca
     1501 ggcctgcgcg cgggacgtgc cgccgcgaaa catctcgttg acgaactggc tcgcgacgac
     1561 gttgcgcacg cgcgccagca cgcgcggcgg aatcgacgcg ccgggatgcc tgccgtcgcc
     1621 ctgaaacacc ttcacgcgcg ccgcgattcc cgcttgcctc gcccgcatcc gggcggcctt
     1681 gcacatcgat ggattgcgat ccagccccca cccgacgaat tcacgatcgt ccgccgccag
     1741 cgcgtgcagc agcgtaccgg cgccgcatcc gagatccagt acgtgattca ggtgcaattg
     1801 gctgatgatc gcggcgagcg ccccgccgcc cgcctcgctc gcctcgaagg cgcgcgcgtg
     1861 cgcgacgagg tcgactgtcg tgcccgccag cgccggccgc cggagcagcg tcgccagccg
     1921 cgaagcgtta tcggcatagg cgccggcata caggttcagc aggaaacgcg cttgcgccgt
     1981 gtagtgctcg gaagccgaga atcggtcgcc ggtcttgcgg atcaggttcg ttctcgatgc
     2041 cgcgtattcg aggacgcctc gcaacaactc gggatcgaga tcgcacgcgg cgctcagcgt
     2101 ttgcgccgtc gtcggctccg ccatcgcatc cacgacgccg agcgtttgca gcgcggcgac
     2161 ggcatgcgcc agttgaagac cttccgcgag atccagcgcg gaccagcgtt cgagtttccg
     2221 acgagccatg agcgaaatga atttagcggg ccggcaccaa aatgcctctg cgcagcagcg
     2281 gctcgaagat cgaagcgtcc acgccgtcat tgcgggtgac gtgcgccagc cattgcgcag
     2341 cggcgcggca cgaacggggc gccctgaaca gatcgaggat cgcggcaatg ccggactcca
     2401 cccaatagcc ggtgacggct tcgccgtccg cgcggacgat ataggcgccg agcccgccga
     2461 catccgcgtc gccgagcgcc ggcgcaccgt cgttcgagcg ccggagccga tcgaccaacg
     2521 cctcgcaatt gtggatgtcc tccagcaggc cgacgttttc ggccagcatg caaggccggt
     2581 ccggctcata gggcccttcg gcgacgacgt ccgccggagc gcgggccgcg gacagccgca
     2641 acgcgtaccg gaaaagagaa tgcaggtgat ggccgcggcc gaatcgcgcg gccacgaaag
     2701 cctccagccc cgccgcatcc ggcagcgcgc cgggcgacgt ttcgtccgcg aaccgtcgcc
     2761 atgccgatat cagccggctc agccgtccgc cgaattcccg caaagcgtac gcgaggatga
     2821 tcggccccgc gcggcgcagc acatccaccg cctcgacggc aaagatgtgt ctcgcgcgcg
     2881 gcagcgcggc ggggtagtaa tagtaggttt gaaatatgtc gggatggtcg agcacctcgc
     2941 gctcgtcctc cggacccagc aggcgcgtgt tgtaaggccc gccatagccg tcgtaggcga
     3001 tctgcgcccc gtgttcgtcg aacagcggcg tgccgggctc cggcgcgagc atgtgcagtt
     3061 gcgtcaggca ggacggccgc cgcgcgcagc gcccgatcat gtccagcgtg tcgtcatggt
     3121 cctgcccgct ttcctgcgga tagccggtga tgaacgacgc ggtcgtctcg attccgaacg
     3181 aatccgccgc ggccagaatg ggctcgacca accgcaagtc caaccgcttc cggcagattg
     3241 tctgcatgcg ttcggagccg gtctccacgc cgaaataaag attcacgcag cccgcgtcgg
     3301 ccatcttcct cagcaatgct tcgtcgacgc aatcgatccg cgccgacgcg cgccaccggt
     3361 agccgcgccc ggccaccgcc tcgcaaaacg ccatcacctt gtggcgattc acggtaaaca
     3421 tgtcatggtc gagcttgaag tcggacacgc gatagcgccg atgaaggatg tccagctcgt
     3481 gcaccaggcg ctcggcggac ttgaggcgaa agctgcgctg aaagaagccc gccgtcgaac
     3541 agaacgtgca tgcgaacgga cagccgcggc cggcctcgat gcgcagcatc gagaggccca
     3601 gatcgtcgac cggataatgg tcgtacgacg cgatcggcag ccggtccagt tcctcgacct
     3661 tgggcttgcc gtcggtgaag cgcaacggcg caccgcagcc cgtcgcgcgc cagctcaagc
     3721 cggggataac atcgaatgtc cgctgctcga ggcagtcgag cacggcggga aggatctcgt
     3781 ccgcttcgta gcgaacgatg atgtcgaact gcgggaatcg ctcgaggatc tgccgatgca
     3841 gcatcgtcgc gtgcgggccg ccgagcagga ccggcaaatc gggctcgcgc cgcttcagca
     3901 gcgccgcgac attgagcgcg aacagaaaac tgcagccgag cgcggtgaag ccgaccgcgt
     3961 ccgggcgctc ggcgaggatc gcgcggctcg cccgctcgta aagcgtcgca tcgtaagcga
     4021 gcgtgccgtc ccggatcagt cgcttcggat catagatccg cacgcgatgg ccgtcggcct
     4081 cggcgaccgc ggccagcgcg agcagcccga gatgcggatg cacgtcgaga taggcgagcg
     4141 agccctcttc cggcatgacg agattgtcga cgagcagcag cttcatgccg cggcgcatcc
     4201 gagcggccgc gcgaatcgca cgatcccgat gccggccagt tggtcgagcg aactcaggaa
     4261 ctgcggcagc agacggcgat tgccgagcaa ccgatggcag atgtcggcgg cggaccgcct
     4321 gccgtcaatc gcttcgatcg cgaagtaacc gagcgtatcg acccgcagca accgtatttc
     4381 gcccgtttcg gcggagcgcc aatagcagca gtaagtgtcg acggcgggaa ccgcgtcgag
     4441 cggcggctcc ttctgatgaa gcgccgccgc cagatcgtca ggattgcagc gcatcacgtg
     4501 ttgaacgacg gagccgttga gcgtcggaat gccgccggcc gtcgcgcgcg cccgatgacg
     4561 cgcatcggga agcggcgcgt cgcccgcttg ccgcaggcat gcgagcgcgt attcatggcg
     4621 caggatgtcc cacagcagaa cgtgattgcg ttgcgcggga tcgagccatc tatcgagaaa
     4681 cgcaatcaga tcgcgcgcgc gctcctgcgg cgtggcggcg aatacgtggc cgctctccgc
     4741 gcagtccgac gcatacgcgg cgaatacctc gatctcgagg ccgaccacgc tcagcagcct
     4801 gaagctcagc ggaaaatctc cgcgcaggcc gttgtggcgc accttcaggc tcaacccgac
     4861 gaaatgctcc aatgcgcgcc aatcgagatc gccgggcgcg atgcccgcgc cgcgcagcag
     4921 gtccggatgc cgccgccaat gctcgatgag ctgcggatcc ttggcgctgg ccgcgatcag
     4981 cgcgtgtatc gtccttgcgt tcatacaagc cccatcagcg cgcgcgcgcg ttcgatgtgg
     5041 ctcgtgagga ccgacaacgg gaagtatggc gaccagttgt attcgaaggt cagcgcgcga
     5101 ggccgcgcga cggcgagcag gcgctcgagc agttcgaaga ggatcggcga cgccggcagc
     5161 gaatgatcgt cccaggccgc gccggactgg atgctcatgc cggacaggtg aacttcgacc
     5221 acgcgctcga gcggcagccg ggcgaattcc cggtgcacgt cggcgcccgt gttgtacgcc
     5281 gtgatcagaa aatggctcag atccaggagc aagccgacat cgcaccggtc cgcgatcgcg
     5341 cgaatgaagt cggtcatgcc catcgtgctg ccgggaatcg aaaagtattg gggcgaattt
     5401 tccacgatca gcggaaccgg aaagcgcgga gcgagcacgg cgagattatc ggcgacgcgc
     5461 cgcaccgtct cgtcgctcaa ctgagggcac agcgtgtaag acagcgacgt gggctcatgg
     5521 gtcccaccca gcgcctcgcc gatgggatcg gccgagatgt acgcgagatg ttcgccgatc
     5581 cacggcgtgc cggtgcgcac ggccgtgcgc tcgattgcgt cgacggtcga atcgtcgggc
     5641 ggcacgaatc ccgcaacgga caagctggcg cagtgcagaa cgaacggaat ggtttgctga
     5701 agctcggcaa tcgtcggcga gaatcgcaag agctcgaacg gcacttcgat gtaatcgacg
     5761 agatgcgcat gccgtgcggc gaattccgcc atgccgggcc cgtacgcaag gccgatccgg
     5821 ggcgccccgg catcgcgatc gaacgctttc ataggcttcc cgcgccttca ggcgccgaac
     5881 cgcagcagtc cagatttatc gcccgccatt gcgcgagcgc acgccgaacg cctcgcgttc
     5941 ggcggcgcgg cttctctgcg aagcccgagc atcgaggcga cgagaatgcg gcgcggccgg
     6001 caaggcccgc gcgcgccgcc ggacgtcagt cgtagagata gccctcgccc atgcggccgc
     6061 cggcgagatc caccttgctc atcgtgatcg tcagcttgcc gttttccagg catttctgga
     6121 tggccttgat ctttttcgcg tccaccggca tatccagcgt catcttgggc agcttccctt
     6181 cgatggaaac ggaaacgccc tttgtttttt tgggtttcgt ggccatgatc gttcctttgt
     6241 tgtgggcagg acgcgacgct cagctgtgag tgtggaataa cccccgaaaa tcgccatgcg
     6301 aatcgatgac cgaaaaaatc ccgtcgggtg tgcatgccga tttgcttctg attaaaggat
     6361 gcgccccggt aaatgtcaag aaagccccgg cgcgaaatgg aacagctcgg cgacaggccc
     6421 gcgccatttc ggcgacggcg tgtcgcggcg ttcgtcgacg gggtgcgccg tgagcgtcgg
     6481 gtcgcctttc ggcatcagca tgatatgaat gcgccagtcg ataaactgtt cgatatccaa
     6541 aacgaaatcg aatcgcgata gcagcgtcga cagcacttcg ccaatcagcg acagcgcgag
     6601 attccgcccc gcgcagaaat ggccgccggc gccgaatgga aaatactcgt acggcgacgg
     6661 cctcgccgtc tcccatcggg acggcgagaa tgcatgcggg tgcgcaaacg cgttggcctc
     6721 gcggtgcacg aggaacgggc acacgacaat ctcgcagcgc gcgggaagcg cgacgccttg
     6781 caacgaaacc gcctgcgtcg tggcgcggac catcagcgcg ttgggcgtca ggagccgaag
     6841 cgtctcgttg atgacgcgct cgagcaatgg cgcgtcgtac ggcgaagccg gcgacgatgc
     6901 gcgggcggcg ctttccgcgc gaagcgcgcg ccgcagatcg ggcaactgcg acagaacgag
     6961 cagcagccac gccagcgata tcgccaccgg ctccgtgctc gacacgaaca tcacgttggc
     7021 gtggccgacg atctcatcct cggaaagcgc gggcgaaccc ggctggccgg ccgtcgccag
     7081 ccgctggagc agccccgcgc actcgccggc gggcgccttt cgataccgcc ggattcgcgc
     7141 ccgcaacgcg cgatcgagtt gccgcccggc gccgatcagc gcgtcccggt actgcaacgg
     7201 atcgtgcgcg ttgggcgaag acgcctcgcg ccgcaacgtg aaatacgcgc ttagcagaaa
     7261 cgtcagttcg gcatccttct cacactgcgg cccaagaaaa agacgggacg ccaacttggc
     7321 tgtcagctcg cgcattcggc tcacgacgga aatccggccg tccccgttca gcatttcgca
     7381 aaagcggctc aaatccgttt gcattccgga tcgatgcgcg tcgaacagcg cgcggttggc
     7441 cgttcccgtc aggaggcgct tgtgccggcc gtgctccggc tcgcgcatgc tgtgcaagcc
     7501 gcggttgagg ttgaccagat tcttcggcaa cgccatcttc gccgccgctg acatcggcag
     7561 cgcgaaggtg tccgtctcgg tcaagacttg tcgaagccga tgttcgccga acacggcgat
     7621 cacgccgctg caatcgctcg cgcgagagaa aatcggaccg tgctcccgca gcacgaagac
     7681 gtcgccgcat tgcgcgcgcg ctcgtgcgag aaaagcgagc ggatccgcaa agagcgaatc
     7741 ggtgctgtcg actcgcggaa gcgacagagg gaggccattc atttgcgcgc tgtgtccgtg
     7801 aacgattcgt gccgatcgtg gctacgccgg ttgatcgcta cagcatggct gccgtcacag
     7861 tatcacgtct tccgaagccg cccggccggt accgcccgag cgcgaacgca acgccgccgc
     7921 gcaatacggc atgccccggg acggataaac atccgccatc gaacgaacgg gccagggccc
     7981 gggttcgcca tgccgccttc ggccgtcgcc gtcgccgtcg ccccaccctt gcacgcggca
     8041 ttccggcatc gtcgcgccct ccgcgtgtcg gtaccgggcg ggacatcgcc cgtccggcca
     8101 tcgcactccg ccccatcgtt ccccgtttaa attccgttga ctttatatct ttcgatatat
     8161 atctttagat acatctaaag acacaggagg cggacatgcg tcacgctcac cgtttttcgc
     8221 gccgcgaatg cggcatgtcc gattcgttcg gacacagccc gttcgagatg tttcgtcaga
     8281 tgttcggcgg ccatcgccgc ggcggcggcc ggcatggctt cggcccattc ggcggcttcg
     8341 gcggcttcgg cggcggcggc cccggtgggt tcggcggctt cggagacgac gcgatgccgc
     8401 gcggccgtca attcagcgcc gacgatctgc agttgctgct gctcgcgctg ctcgccaagc
     8461 agccgagcca cggctacgag ctgatcaagg cgctcgacac gcgctcgagc ggcttctacg
     8521 cgccgagccc cggcatggtg tatccggcgc tcacgtatct ggaagaactc ggctacgtga
     8581 cggtgcaggc cgaaggcaac cgcaagcgtt acgcgctcgc cgacgcgggc cgcgcgcatc
     8641 tcgacgcgca gcgtgaacgc gtcgagatgc tgttcgcgaa gctcgcgcat ctcggccgca
     8701 agatggaatt catgcgtcgc gcgttcgccg gcgaagcgcc cgccgcgccc gaggacgaca
     8761 cgcgcggctg gctgccggaa ttcgtcgaag cacggatggc gctcaagcaa gcgctctttc
     8821 gcagaagcgg cgcaaacgcc gacgagcaac gccgcatcgc ggccattttg cggcgcgccg
     8881 tcgccgacat cgaaggccgc gcgcaaggct gaatcgcgcc gtttccggct catcaaggag
     8941 aaccgatcga catgacccaa ccgactaccg agcgcgcggt cgtacgcgtg cgtcatacgc
     9001 tgaaattccg cctgctgcag gtcaagcgcg tcgtcgacgt gacgccgcat ctgaagcgcg
     9061 tgacgctggc aggcgacgaa ctcgacgatt tcgaatcggc gtcgttcgac gatcacgtga
     9121 aagtcttctt cccgccgccc ggcgaagacg cacccgcgcg ccccgaattc ggcccgaacg
     9181 gcatcgtgtt tccgcaggac aggccgcgcc ccgtcgcacg cgatttcacg ccgcgccgct
     9241 atgatcgcgc cgcgcgagag ctcgatctcg atttcgtgct gcatcatccg gggccggctt
     9301 cgcagtgggc cgctcaggcg cgcgtcgggc aaacgctcgg catcggcggg ccgcgcggtt
     9361 cgttcgtgat cccgaaggat ttcgactggc atctgctgat cggcgacgac accgcattgc
     9421 ccgccgtcgc gcgccggctc gaggaattgc cggccggcac gcgcgtcgcg gcggtgctcg
     9481 aagtggccga tccgtcggcg cagatcgcat tcgaaaccga tgcgaacctg tatgtggtat
     9541 ggcgacatcg cggcgatgcg gcgagcggcg agcgcggcgc gagccggctg gtcgacgcat
     9601 tgcgcgaact gccgctgccg gcgggcgaag gctacgtatg ggcggcgggc gaggcggccg
     9661 cgatgcgcgg cgtgcgcgag catctgtgcc gcgagcgcgg cgtcgcgaaa tcgcggattc
     9721 gcgcatccgc ttactggaag cacggtgcga tcgccgtgca cgaatcgctc gaagactgat
     9781 ccgcggccgg ccgctgcgcg cgcgaatatc gcccggcgat tcacgcgcgc gatccaagcg
     9841 cacggcgccg ccggcacccg catgcagtat tgcaatgcac gcgatagcgg ccggcaggca
     9901 gcacacgcgc gtacgcctcg accggccatc gccgaaagcg aaagccaaaa ccgcaaaccc
     9961 cttcacgccg ccgctacgtt cgccgcgtcg cgttttcgcc gtttcgtatg agctgaatcg
    10021 cgcgcgtacc gctgtcgcat cgcatcggct tgtcttgatt gtcttgaaat agccgcgcac
    10081 cgccacgctc gcgatacgtc gctcatggca agcggctctg gttcgcgccc gatcgcaccg
    10141 caccgccgcg ccgctcgagc gcagccgcgc cgcgctgacc catcgcatcg cacgccttcg
    10201 ctgccattcc gcgcgccgac aaagccgttt cgttcgcttc tccgccgttt tcccgccgat
    10261 caacgccgca gcgacggcgg ccggcgcgcg cgttcaggtt ccatcgtccg cttgcatcgc
    10321 gcgatgccct cgcatccgcg cgtctccaca cgagcggagt tgcatccgca tcgcccgtgt
    10381 cctataccta tgtcgacggc acatggcgac gccgatggcg gcacaacggg gcgcgcgccg
    10441 ccgtgaccgg atagggaaga gcacggcgcg caccgcggta ttttcgcgtg tcgaacgaga
    10501 acgagaccgc gcggcttcgc gcgtcgccac ccggatacgc gtccatgact cgccaccaca
    10561 cccacgccgg cattccgaga acgctcaaaa tcgtcggcta ttgcgggctc gccgcaatcg
    10621 cgctcgcgct gttcgtcgcg atgtgcgcga tcctcaaaga cagcgcgctc gatcgcgaag
    10681 ccgcgcgcca gttgcagaac gggaacgtcg aattgcacgg cggcgccgat gtcgagccgg
    10741 gctcgacaaa cgacgccgcc caggcgagaa cgccgggttg atccggattc ctgccgatcg
    10801 gcgtcagtcg atgcggatcg gccgctcgtg caatcgcgtg aacgcgaccg cg
//
