LOCUS       NZ_CP009272            10852 bp    DNA     circular CON 15-JAN-2024
DEFINITION  Burkholderia pseudomallei MSHR1153 chromosome 2.
ACCESSION   NZ_CP009272
VERSION     NZ_CP009272.1
KEYWORDS    RefSeq.
SOURCE      Burkholderia pseudomallei MSHR1153
  ORGANISM  Burkholderia pseudomallei MSHR1153
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Burkholderia; pseudomallei group.
REFERENCE   1  (bases 1 to 3280677)
  AUTHORS   Bunnell,A., Chain,P.S., Chertkov,O., Currie,B.J., Daligault,H.E.,
            Davenport,K.W., Davis,C., Gleasner,C.D., Johnson,S.L., Kaestli,M.,
            Koren,S., Kunde,Y.A., Mayo,M., McMurry,K.K., Price,E.P.,
            Reitenga,K.G., Robison,R., Rosovitz,M.J., Sarovich,D.S. and
            Teshima,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2014) Bioscience, Los Alamos National Laboratory,
            P.O. Box 1663, MS M888, Los Alamos, NM 87545, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-AUG-2014
            Assembly Method :: Newbler v. 2.6; Velvet v. 1.2.01; Allpaths v.
            39750; Phrap v. SPS - 4.24
            Genome Coverage                   :: 269x
            Sequencing Technology             :: Illumina; 454; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/15/2024 02:42:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,500
            CDSs (total)                      :: 6,424
            Genes (coding)                    :: 6,236
            CDSs (with protein)               :: 6,236
            Genes (RNA)                       :: 76
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 188
            CDSs (without protein)            :: 188
            Pseudo Genes (ambiguous residues) :: 0 of 188
            Pseudo Genes (frameshifted)       :: 110 of 188
            Pseudo Genes (incomplete)         :: 107 of 188
            Pseudo Genes (internal stop)      :: 18 of 188
            Pseudo Genes (multiple problems)  :: 43 of 188
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:48:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1865872
            Orig. end                         :: 1876724
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP009272.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            DNA was obtained from Dr. Richard Robison, Brigham Young University
            (BYU), richard_robison@byu.edu.
FEATURES             Location/Qualifiers
     region          1..10852
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="11"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10852
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10852
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5852](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5852
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(95..331)
                     /locus_tag="Y603_RS25390"
                     /old_locus_tag="Y603_4941"
     CDS             complement(95..331)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196519.1"
                     /locus_tag="Y603_RS25390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Y603_4941"
                     /product="hypothetical protein"
                     /protein_id="WP_009922809.1"
                     /transl_table=11
                     /translation="MTRHHTHAGIPRTLKIVGYCGLAAIALALFIAMCAILKDSALDRE
                     AARQLQNGNVELHGGADVEPGSTNDAAQARTPG"
     gene            525..773
                     /locus_tag="Y603_RS42565"
     CDS             525..773
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194583.1"
                     /locus_tag="Y603_RS42565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_011852397.1"
                     /transl_table=11
                     /translation="MWRRADARASRDASGRWNLNARAGRRRCGVDRRENGGEANETALS
                     ARGMAAKACDAMGQRGAAALERRGGAVRSGANQSRLP"
     gene            complement(1097..1924)
                     /locus_tag="Y603_RS25395"
                     /old_locus_tag="Y603_4942"
     CDS             complement(1097..1924)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /GO_process="GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1200: vibriobactin
                     utilization protein ViuB"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532447.1"
                     /locus_tag="Y603_RS25395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Y603_4942"
                     /product="siderophore-interacting protein"
                     /protein_id="WP_038726504.1"
                     /transl_table=11
                     /translation="MTQPTTERAVVRVRHTLKFRLLQVKRVVDVTPHLKRVTLAGDELD
                     DFESASFDDHVKVFFPPPGEDAPARPEFGPNGIVFPQDRPRPVARDFTPRRYDRAAREL
                     DLDFVLHHPGPASQWAAQARVGQTLGIGGPRGSFVIPKDFDWHLLIGDDTALPAVARRL
                     EELPAGTRVAAVLEVADPSAQIAFETDANLYVIWRHRGDAASGERGASRLVDALRELPL
                     PAGEGYVWAAGEAAAMRGVREHLCRERGVAKSRIRASAYWKHGAIAVHESLED"
     gene            complement(1964..2668)
                     /locus_tag="Y603_RS25400"
                     /old_locus_tag="Y603_4943"
     CDS             complement(1964..2668)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548999.1"
                     /locus_tag="Y603_RS25400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Y603_4943"
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_004548999.1"
                     /transl_table=11
                     /translation="MRHAHRFSRRACGMSDSFGHSPFEMFRQMFGGHRRGGGRHGFGPF
                     GGFGGGPGGFGGFGDDAMPRGRQFSADDLQLLLLALLAEQPSHGYELIKALDTRSSGFY
                     APSPGMVYPALTYLEELGYVTVQAEGNRKRYALADAGRAHLDAQRERVEMLFAKLAHLG
                     RKMEFMRRAFAGEAPAAPEDDTRGWLPEFVEARMALKQALFRRSGANADEQRRIAAILR
                     RAVADIEGRAQG"
     gene            3071..4477
                     /locus_tag="Y603_RS25405"
                     /old_locus_tag="Y603_4944"
     CDS             3071..4477
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009981062.1"
                     /locus_tag="Y603_RS25405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Y603_4944"
                     /product="cytochrome P450"
                     /protein_id="WP_009922815.1"
                     /sec_met_domain="p450 (E-value: 4.2e-34, bitscore: 115.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGLPLSLPRVDSTDSLFADPLAFLARARAQCGDVFVLREHGPIF
                     SRASDCSGVIAVFGEHRLRQVLTETDTFALPMSAAAKMALPKNLVNLNRGLHSMREPEH
                     GRHKRLLTGTANRALFDAHRSGMQTDLSRFCEMLNGDGRISVVSRMRELTAKLASRLFL
                     GPQCEEDAELTFLLSAYFTLRREASSPNAHDPLQYRDALIGAGRQLDCALRARIRRYRK
                     VPAGECAGLLQRLATAGQPGSPALSEDEIVGHANVMFVSSTEPVAISLAWLLLVLSQLP
                     DLRRALRAESAARASSPALPYDAPLLERVINETLRLLTPNALMVRATTQAASLQGVALP
                     ARCEIVVCPFLVHREANAFARPHAFLPSRWETARPSPYEYFPFGAGGHFCAGRNLALSL
                     IGEVLSTLLSRFDFVLDIEQFIDWRIHIMLMPKGDPTLMAHPVDERRDTPSPKWRGPVA
                     ELFHFAPGLS"
     misc_feature    4292..4294
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    4532..4546
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.99"
                     /tool="antismash"
     gene            4627..4827
                     /locus_tag="Y603_RS25410"
                     /old_locus_tag="Y603_4945"
     CDS             4627..4827
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525608.1"
                     /locus_tag="Y603_RS25410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Y603_4945"
                     /product="hypothetical protein"
                     /protein_id="WP_004201048.1"
                     /transl_table=11
                     /translation="MATKPKKTKGVSVSIEGKLPKMTLDMPVDAKKIKAIQKCLENGKL
                     TITMSKVDLAGGRMGEGYLYD"
     misc_feature    complement(4641..4650)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.0"
                     /tool="antismash"
     gene            5001..5852
                     /locus_tag="Y603_RS25415"
                     /old_locus_tag="Y603_4946"
     CDS             5001..5852
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603954.1"
                     /locus_tag="Y603_RS25415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Y603_4946"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_004196528.1"
                     /sec_met_domain="DUF692 (E-value: 9e-35, bitscore: 117.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAFDRDAGAPRIGLAYGPGMAEFAARHAHLVDYIEVPFELLRFS
                     PTIAELQQTIPFVLHCASLSVAGFVPPDDSTVDAIERTAVRTGTPWIGEHLAYISADPI
                     GEALGGTHEPTSLSYTLCPQLSDETVRRVADNLAVLAPRFPVPLIVENSPQYFSIPGST
                     MGMTDFIRAIADRCDVGLLLDLSHFLITAYNTGADVHREFARLPLERVVEVHLSGMSIQ
                     SGAAWDDHSLPASPILFELLERLLAVARPRALTFEYNWSPYFPLSVLTSHIERARALMG
                     LA"
     gene            5849..6682
                     /locus_tag="Y603_RS25420"
                     /old_locus_tag="Y603_4947"
     CDS             5849..6682
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543057.1"
                     /locus_tag="Y603_RS25420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Y603_4947"
                     /product="hypothetical protein"
                     /protein_id="WP_043275920.1"
                     /transl_table=11
                     /translation="MNARTIHALIAASAKDPQLIERWRRHPDLLRGAGIAPGDLDWRAL
                     EHFVGLSLKVRHNGLRGDFPLSFRLLSVVGLEIEVFAAYASDCAESGHVFAATPQERAR
                     DLIAFLDRWLDPAQRNHVLLWDILRHEHALACLRQAGDAPLADARPAARHRARATAGGI
                     PTLNGSVVQHVMRCNPDDLAAALHQKEPPLDAVPAVDTYCCYWRSAETGEIRLLRVDTL
                     GYCAIEAIDGRRSAADICHRLLGNRRLLPQFLSSLDQLAGIGIVRFARPLGCAAA"
     gene            6679..8622
                     /locus_tag="Y603_RS25425"
                     /old_locus_tag="Y603_4948"
     CDS             6679..8622
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004549365.1"
                     /locus_tag="Y603_RS25425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Y603_4948"
                     /product="radical SAM protein"
                     /protein_id="WP_043276329.1"
                     /sec_met_domain="PF04055 (E-value: 7.5e-14, bitscore: 49.8,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLLLVDNLVMPEEGSLAYLDVHPHLGLLALAAVAEADGHRVRIY
                     DPKRLIRDGTLAYDATLYERASRAILAERPDVVGFTALGCSFLFALNVAALLKRREPDL
                     PVLLGGPHATMLHRQILERFPQFDIIVRYEADEILPAVLDCLEQRTFDVIPGLSWRATG
                     RGAPLRFTDGKPKVEELDRLPIASYDHYPVDDLGLSMLRIEAGRGCPFACTFCSTAGFF
                     QRSFRLKSAERLVHELDILHRRYRVSDFKLDHDMFTVNRHKVMAFCEAVAGRGYRWRAS
                     ARIDCVDEALLRKMADAGCVNLYFGVETGSERMQTICRKRLDLRLVEPILAAADSFGIE
                     TTASFITGYPQESGQDQDDTLDMIGRCARRSSCLTQLHMLAPEPGTPLFDEHGAQIAYD
                     GYGGPYNTRLLGPGDEREVLDHPDIFQTYYYYPAALPRARHIFAVEAVDVLRRAGPIIL
                     AYALREFGGRLSRLISAWRRFADETSPGALPDAAGLEAFVAARFGRGHHLHSLFRYALR
                     LSAARAPADVVAEGPYEPDRPCMLAENVGLLEDIHNCEALVDRLRRSNDGAPALGDADV
                     GGLGAYIVRAEGEAVTGYWVESGIAAILDLFRAPRSCRAAAQWLAHVTRNDGVDASIFE
                     PLLRRGILVPAR"
     gene            8636..9625
                     /locus_tag="Y603_RS25430"
                     /old_locus_tag="Y603_4949"
     CDS             8636..9625
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852411.1"
                     /locus_tag="Y603_RS25430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Y603_4949"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_004532676.1"
                     /transl_table=11
                     /translation="MARRKLERWSALDLAEGLQLAHAVAALQTLGVVDAMAEPTTAQTL
                     SAACDLDPELLRGVLEYAASRTNLIRKTGDRFSASEHYTAQARFLLNLYAGAYADNASR
                     LATLLRRPALAGTTVDLVAHARAFEASEAGGGALAAIISQLHLNHVLDLGCGAGTLLHA
                     LAADDREFVGWGLDRNPSMCKAARMRARQAGIAARVKVFQGDGRHPGASIPPRVLARVR
                     NVVASQFVNEMFRGGTSRAQAWLRRMRRLLPDRLLVIADYYGRLGHGFQTPHRETLLHD
                     YAQLISGQGVPPPNADAWLAMYRAAGCRPLHVIEDRAATTRFVHLVAL"
     gene            complement(9797..10417)
                     /locus_tag="Y603_RS25435"
                     /old_locus_tag="Y603_4950"
     CDS             complement(9797..10417)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894570.1"
                     /locus_tag="Y603_RS25435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Y603_4950"
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_004194492.1"
                     /transl_table=11
                     /translation="MNWIAFSHGAMLCASLIVTIGAQNAFILRQGIMRSHVGKIVLLCT
                     LSDMLLIGAGVGGASVLVERYPAFVHAVLYVGLAYLAWFGLSALRRAFKPGHETLDATG
                     AVAAPPAQRAWPIVLMTLAFTWLNPHVYLDTFLLIGTAGAREPEGARGAFAIGAMAVSA
                     VWFVALGYGARLLAPWFKRALAWRVLDGAIGGMVLFLAAVQLR"
ORIGIN
        1 gaccgggtcg gcgaatcggt gcacgcggtc gcgttcacgc gattgcacga gcggccgatc
       61 cgcatcgact gacgccgatc ggcgggaatc cggatcaacc cggcgttctc gcctgggcgg
      121 cgtcgttcgt cgagcccggc tcgacatcgg cgccgccgtg caattcgacg ttcccgttct
      181 gcaactggcg cgcggcttcg cgatcgagcg cgctgtcttt gaggatcgcg cacatcgcga
      241 tgaacagcgc gagcgcgatt gcggcgagcc cgcaatagcc gacgattttg agcgttctcg
      301 gaatgccggc gtgggtgtgg tggcgagtca tggacgcgta tccgggtggc gacgcgcgaa
      361 gccgcgcggt ctcgttctcg ttcgacacgc gaaaataccg cggtgcgcgc cgtgctcttc
      421 cctatccggt cacggcggcg cgcgccccgt tgtgccgtca tcggcgtcgc catgtgccgt
      481 cgacataggt ataggacacg ggcgatgcgg atgcaactcc gctcgtgtgg agacgcgcgg
      541 atgcgagggc atcgcgcgat gcaagcggac gatggaacct gaacgcgcgc gccggccgcc
      601 gtcgctgcgg cgttgatcgg cgggaaaacg gcggagaagc gaacgaaacg gctttgtcgg
      661 cgcgcggaat ggcagcgaag gcgtgcgatg cgatgggtca gcgcggcgcg gctgcgctcg
      721 agcggcgcgg cggtgcggtg cgatcgggcg cgaaccagag ccgcttgcca tgagcgacgt
      781 atcgcgagcg tggcggtgcg cggctatttc aagacaatca agacaagccg atgcgatgcg
      841 acagcggtac gcgcgcgatt cagctcatac gaaacggcga aaacgcgacg cggcgaacgt
      901 agcggcggcg tgaaggggtt tgcggttttg gctttcgctt tcggcgatgg ccggtcgagg
      961 cgtacgcgcg tgtgctgcct gccggccgct atcgcgtgca ttgcaatact gcatgcgggt
     1021 gccggcgacg ccgtgcgctt ggatcgcgcg cgtgaatcgc cgggcgatat tcgggcgcgc
     1081 agcggccggc cgcggatcag tcttcgagcg attcgtgcac ggcgatcgca ccgtgcttcc
     1141 agtaagcgga tgcgcgaatc cgcgatttcg cgacgccgcg ctcgcggcac agatgctcgc
     1201 gcacgccgcg catcgcggcc gcctcgcccg ccgcccatac gtagccttcg cccgccggca
     1261 gcggcagttc gcgcaatgcg tcgaccagcc ggctcgcgcc gcgctcgccg ctcgccgcat
     1321 cgccgcgatg tcgccatatc acatacaggt tcgcatcggt ttcgaatgcg atctgcgccg
     1381 acggatcggc cacttcgagc accgccgcga cgcgcgtgcc ggccggcaat tcctcgagcc
     1441 ggcgcgcgac ggcgggcaat gcggtgtcgt cgccgatcag cagatgccag tcgaaatcct
     1501 tcgggatcac gaacgaaccg cgcggcccgc cgatgccgag cgtttgcccg acgcgcgcct
     1561 gagcggccca ctgcgaagcc ggccccggat gatgcagcac gaaatcgaga tcgagctcgc
     1621 gcgcggcgcg atcatagcgg cgcggcgtga aatcgcgtgc gacggggcgc ggcctgtcct
     1681 gcggaaacac gatgccgttc gggccgaatt cggggcgcgc gggtgcgtct tcgccgggcg
     1741 gcgggaagaa gactttcacg tgatcgtcga acgacgccga ttcgaaatcg tcgagttcgt
     1801 cgcctgccag cgtcacgcgc ttcagatgcg gcgtcacgtc gacgacgcgc ttgacctgca
     1861 gcaggcggaa tttcagcgta tgacgcacgc gtacgaccgc gcgctcggta gtcggttggg
     1921 tcatgtcgat cggttctcct tgatgagccg gaaacggcgc gattcagcct tgcgcgcggc
     1981 cttcgatgtc ggcgacggcg cgccgcaaaa tggccgcgat gcggcgttgc tcgtcggcgt
     2041 ttgcgccgct tctgcgaaag agcgcttgct tgagcgccat ccgtgcttcg acgaattccg
     2101 gcagccagcc gcgcgtgtcg tcctcgggcg cggcgggcgc ttcgccggcg aacgcgcgac
     2161 gcatgaattc catcttgcgg ccgagatgcg cgagcttcgc gaacagcatc tcgacgcgtt
     2221 cacgctgcgc gtcgagatgc gcgcggcccg cgtcggcgag cgcgtaacgc ttgcggttgc
     2281 cttcggcctg caccgtcacg tagccgagct cttccagata cgtgagcgcc ggatacacca
     2341 tgccggggct cggcgcgtag aagccgctcg agcgcgtgtc gagcgccttg atcagctcgt
     2401 agccgtggct cggctgctcg gcgagcagcg cgagcagcag caactgcaga tcgtcggcgc
     2461 tgaattgacg gccgcgcggc atcgcgtcgt ctccgaagcc gccgaaccca ccggggccgc
     2521 cgccgaagcc gccgaatgga ccgaagccat gccggccgcc gccgcggcga tggccgccga
     2581 acatctgacg aaacatctcg aacgggctgt gtccgaacga atcggacatg ccgcatgcgc
     2641 ggcgcgaaaa acggtgagcg tggcgcatgt ccgcctcctg tgtctttaga tgtatctaaa
     2701 gatatatatc gaaagatata aagtcaacgg aatttaaacg gggaacgatg ggggcggagt
     2761 gcgatggccg gacgggcgat gtcccgcccg gtaccgacac gcggagggcg cgacgatacc
     2821 ggaatgccgc gtgcaagggt ggggcgacgg ccgaaggcgg catggcgaac ccgggccctg
     2881 gcccgttcgt tcgatggcgg atgtttatcc gtcccggggc atgccgtatt gcgcggcggc
     2941 gttgcgttcg cgctcgggcg gtaccggccg ggcggcttcg gaagacgtga tactgtgacg
     3001 gcagccatgc tgtagcgatc aaccggcgta gccacgatcg gcacgaatcg ttcacggaca
     3061 cagcgcgcaa atgaatggcc tccctctgtc gcttccgcga gtcgacagca ccgattcgct
     3121 ctttgcggat ccgctcgctt ttctcgcacg agcgcgcgcg caatgcggcg acgtcttcgt
     3181 gctgcgggag cacggtccga ttttttctcg cgcgagcgat tgcagcggcg tgatcgccgt
     3241 gttcggcgaa catcggcttc gacaagtctt gaccgagacg gacaccttcg cgctgccgat
     3301 gtcagcggcg gcgaagatgg cgttgccgaa gaatctggtc aacctcaacc gcggcttgca
     3361 cagcatgcgc gagccggagc acggccggca caagcgcctc ctgacgggaa cggccaaccg
     3421 cgcgctgttc gacgcgcatc gatccggaat gcaaacggat ttgagccgct tttgcgaaat
     3481 gctgaacggg gacggccgga tttccgtcgt gagccgaatg cgcgagctga cagccaagtt
     3541 ggcgtcccgt ctttttcttg ggccgcagtg tgaggaggat gccgaactga cgtttctgct
     3601 aagcgcgtat ttcacgttgc ggcgcgaggc gtcttcgccc aacgcgcacg atccgttgca
     3661 gtaccgggac gcgctgatcg gcgccgggcg gcaactcgat tgcgcgttgc gggcgcggat
     3721 ccggcggtat cgaaaggtgc ccgccggcga gtgcgcgggg ctgctccagc ggctggcgac
     3781 ggccggccag ccgggttcgc ccgcgctttc cgaggatgag atcgtcggcc acgccaacgt
     3841 gatgttcgtg tcgagcacgg agccggtggc gatatcgctg gcgtggctgc tgctcgttct
     3901 gtcgcagttg cccgatctgc ggcgcgcgct tcgcgcggaa agcgccgccc gcgcatcgtc
     3961 gccggctttg ccgtacgacg cgccattgct cgagcgcgtc atcaacgaga cgcttcggct
     4021 cctgacgccc aacgcgctga tggtccgcgc cacgacgcag gcggcttcgt tgcaaggcgt
     4081 cgcgcttccc gcgcgctgcg agattgtcgt gtgcccgttc ctcgtgcacc gcgaggccaa
     4141 cgcgtttgcg cgcccgcatg cattcttgcc gtcccgatgg gagacggcga ggccgtcgcc
     4201 gtacgagtat tttccattcg gcgccggcgg ccatttctgc gcggggcgga atctcgcgct
     4261 gtcgctgatt ggcgaagtgc tgtcgacgct gttatcgcga ttcgatttcg ttttggatat
     4321 cgaacagttt atcgactggc gcattcatat catgctgatg ccgaaaggcg acccgacgct
     4381 catggcgcac cccgtcgacg aacgccgcga cacgccgtcg ccgaaatggc gcgggcctgt
     4441 cgccgagctg ttccatttcg cgccggggct ttcttgacat ttaccggggc gcatccttta
     4501 atcagaagca aatcggcatg cacacccgac gggatttttt cggtcatcga ttcgcatggc
     4561 gattttcggg ggttattcca cactcacagc tgagcgtcgc gtcctgccca caacaaagga
     4621 acgatcatgg ccacgaaacc caaaaaaaca aagggcgttt ccgtttccat cgaagggaag
     4681 ctgcccaaga tgacgctgga tatgccggtg gacgcgaaaa agatcaaggc catccagaaa
     4741 tgcctggaaa acggcaagct gacgatcacg atgagcaagg tggatctcgc cggcggccgc
     4801 atgggcgagg gctatctcta cgactgacgt ccggcggcgc gcgcgggcct tgccggccgc
     4861 gtcgcattct cgtcgcctcg atgctcgggt ttcgcagaga agccgcgccg ccgaacgcga
     4921 ggcgttcggc gtgcgctcgc gcaatggcgg gcgataaatc tggactgctg cggttcggcg
     4981 cctgaaggcg cgggaagcct atgaaagcgt tcgatcgcga tgccggggcg ccccggatcg
     5041 gccttgcgta cgggcccggc atggcggaat tcgctgcacg gcatgcgcat ctcgtcgatt
     5101 acatcgaagt gccgttcgag ctcttgcgat tctcgccgac gattgccgag cttcagcaaa
     5161 ccattccgtt cgttctgcac tgcgccagct tgtccgttgc gggattcgtg ccgcccgacg
     5221 attcgaccgt cgacgcaatc gagcgcacgg ccgtgcgcac cggcacgccg tggatcggcg
     5281 aacatctcgc gtacatctcg gccgatccca tcggcgaggc gctgggtggg acccatgagc
     5341 ccacgtcgct gtcttacacg ctgtgccctc agttgagcga cgagacggtg cggcgcgtcg
     5401 ccgataatct cgccgtgctc gctccgcgct ttccggttcc gctgatcgtg gaaaattcgc
     5461 cccaatactt ttcgattccc ggcagcacga tgggcatgac cgacttcatt cgcgcgatcg
     5521 cggaccggtg cgatgtcggc ttgctcctgg atctgagcca ttttctgatc acggcgtaca
     5581 acacgggcgc cgacgtgcac cgggaattcg cccggctgcc gctcgagcgc gtggtcgaag
     5641 ttcacctgtc cggcatgagc atccagtccg gcgcggcctg ggacgatcat tcgctgccgg
     5701 cgtcgccgat cctcttcgaa ctgctcgagc gcctgctcgc cgtcgcgcgg cctcgcgcgc
     5761 tgaccttcga atacaactgg tcgccatact tcccgttgtc ggtcctcacg agccacatcg
     5821 aacgcgcgcg cgcgctgatg gggcttgcat gaacgcaagg acgatacacg cgctgatcgc
     5881 ggccagcgcc aaggatccgc agctcatcga gcgttggcgg cggcatccgg acctgctgcg
     5941 cggcgcgggc atcgcgcccg gcgatctcga ttggcgcgca ttggagcatt tcgtcgggtt
     6001 gagcctgaag gtgcgccaca acggcctgcg cggagatttt ccgctgagct tcaggctgct
     6061 gagcgtggtc ggcctcgaga tcgaggtatt cgccgcgtat gcgtcggact gcgcggagag
     6121 cggccacgta ttcgccgcca cgccgcagga gcgcgcgcgc gatctgattg cgtttctcga
     6181 tagatggctc gatcccgcgc aacgcaatca cgttctgctg tgggacatcc tgcgccatga
     6241 acacgcgctc gcatgcctgc ggcaagcggg cgacgcgccg cttgccgatg cgcgtcccgc
     6301 cgcgcgtcat cgggcgcgcg cgacggccgg cggcattccg acgctcaacg gctccgtcgt
     6361 tcaacacgtg atgcgctgca atcctgacga tctggcggcg gcgcttcatc agaaggagcc
     6421 gccgctcgac gcggttcccg ccgtcgacac ttactgctgc tattggcgct ccgccgaaac
     6481 gggcgaaata cggttgctgc gggtcgatac gctcggttac tgcgcgatcg aagcgatcga
     6541 cggcaggcga tccgccgccg acatctgcca tcggttgctc ggcaatcgcc gtctgctgcc
     6601 gcagttcctg agttcgctcg accaactggc cggcatcggg atcgtgcgat tcgcgcggcc
     6661 gctcggatgc gccgcggcat gaagctgctg ctcgtcgaca atctcgtcat gccggaagag
     6721 ggctcgctcg cctatctcga cgtgcatccg catctcgggc tgctcgcgct ggccgcggtc
     6781 gccgaggccg acggccatcg cgtgcggatc tatgatccga agcgactgat ccgggacggc
     6841 acgctcgctt acgatgcgac gctttacgag cgggcgagcc gcgcgatcct cgccgagcgc
     6901 ccggacgtgg tcggcttcac cgcgctcggc tgcagttttc tgttcgcgct caatgtcgcg
     6961 gcgctgctaa agcggcgcga gcccgatttg ccggtcctgc tcggcggccc gcacgcgacg
     7021 atgctgcatc ggcagatcct cgagcgattc ccgcagttcg acatcatcgt tcgctacgaa
     7081 gcggacgaga tccttcccgc cgtgctcgac tgcctcgagc agcggacatt cgatgttatc
     7141 cccggcttga gctggcgcgc gacgggccgc ggtgcgccgt tgcgcttcac cgacggcaag
     7201 cccaaggtcg aggaactgga ccggctgccg atcgcgtcgt acgaccatta tccggtcgac
     7261 gatctgggcc tctcgatgct gcgcatcgag gccggccgcg gctgtccgtt cgcatgcacg
     7321 ttctgttcga cggcgggctt ctttcagcgc agctttcgcc tcaagtccgc cgagcgcctg
     7381 gtgcacgagc tggacatcct tcatcggcgc tatcgcgtgt ccgacttcaa gctcgaccat
     7441 gacatgttta ccgtgaatcg ccacaaggtg atggcgtttt gcgaggcggt ggccgggcgc
     7501 ggctaccggt ggcgcgcgtc ggcgcggatc gattgcgtcg acgaagcatt gctgaggaag
     7561 atggccgacg cgggctgcgt gaatctttat ttcggcgtgg agaccggctc cgaacgcatg
     7621 cagacaatct gccggaagcg gttggacttg cggttggtcg agcccattct ggccgcggcg
     7681 gattcgttcg gaatcgagac gaccgcgtcg ttcatcaccg gctatccgca ggaaagcggg
     7741 caggaccagg acgacacgct ggacatgatc gggcgctgcg cgcggcgatc gtcctgcctg
     7801 acgcaactgc acatgctcgc gccggagccc ggcacgccgc tgttcgacga acacggggcg
     7861 cagatcgcct acgacggcta tggcgggcct tacaacacgc gcctgctggg cccgggggac
     7921 gagcgcgagg tgctcgacca tcccgacata tttcaaacct actattacta ccccgccgcg
     7981 ctgccgcgcg cgagacacat ctttgccgtc gaggcggtgg atgtgctgcg ccgcgcgggg
     8041 ccgatcatcc tcgcgtacgc tttgcgggaa ttcggcggac ggctgagccg gctgatatcg
     8101 gcatggcgac ggttcgcgga cgaaacgtcg cccggcgcgt tgccggatgc ggcggggctg
     8161 gaggctttcg tggccgcgcg attcggccgc ggccatcacc tgcattctct tttccggtac
     8221 gcgttgcggc tgtccgcggc ccgcgctccg gcggacgtcg tcgccgaagg gccctatgag
     8281 ccggaccggc cttgcatgct ggccgaaaac gtcggcctgc tggaggacat ccacaattgc
     8341 gaggcgttgg tcgatcggct ccggcgctcg aacgacggtg cgccggcgct cggcgacgcg
     8401 gatgtcggcg ggctcggcgc ctatatcgtc cgcgcggagg gcgaagccgt caccggctat
     8461 tgggtggagt ccggcattgc cgcgatcctc gatctgttca gggcgccccg ttcgtgccgc
     8521 gccgctgcgc aatggctggc gcacgtcacc cgcaatgacg gcgtggatgc ttcgatcttc
     8581 gagccgctgc tgcgcagagg cattttggtg ccggcccgct aaattcattt cgctcatggc
     8641 tcgtcggaaa ctcgaacgct ggtccgcgct ggatctcgcg gaaggtcttc aactggcgca
     8701 tgccgtcgcc gcgctgcaaa cgctcggcgt cgtggatgcg atggcggagc cgacgacggc
     8761 gcaaacgctg agcgccgcgt gcgatctcga tcccgagttg ttgcgaggcg tcctcgaata
     8821 cgcggcatcg agaacgaacc tgatccgcaa gaccggcgac cgattctcgg cttccgagca
     8881 ctacacggcg caagcgcgtt tcctgctgaa tctgtatgcc ggcgcctatg ccgataacgc
     8941 ttcgcggctg gcgacgctgc tccggcggcc ggcgctggcg ggcacgacag tcgacctcgt
     9001 cgcgcacgcg cgcgccttcg aggcgagcga ggcgggcggc ggggcgctcg ccgcgatcat
     9061 cagccaattg cacctgaatc acgtactgga tctcggatgc ggcgccggta cgctgctgca
     9121 cgcgctggcg gcggacgatc gtgaattcgt cgggtggggg ctggatcgca atccgtcgat
     9181 gtgcaaggcc gcccggatgc gggcgaggca agcgggaatc gcggcgcgcg tgaaggtatt
     9241 tcagggcgac ggcaggcatc ccggcgcgtc gattccgccg cgcgtgctgg cgcgcgtgcg
     9301 caacgtcgtc gcgagccagt tcgtcaacga gatgtttcgc ggcggcacgt cccgcgcgca
     9361 ggcctggctt cgacgcatgc gccgcctgct gcccgaccgc ctgctcgtga tcgcggacta
     9421 ctacggccgc ctcggccacg gttttcaaac gccgcaccgc gagacgttgc tgcacgacta
     9481 tgcgcagctc atatcggggc aaggcgtgcc gccgcccaat gccgacgcgt ggctcgccat
     9541 gtatcgggcg gccggttgcc ggcctctgca tgtgatcgaa gaccgcgcgg ccacgacgcg
     9601 cttcgtccac ctggtagcgc tgtagcggca tgacgggcgc ggctcggcgg cgccgcttgc
     9661 gccgaccgcg tgcgccggct ggccgattcc ctcggggcgt gcggccatga cgttcgtggc
     9721 tcgctcgccg ccgtgcgccg cgtgccgccg tccgcgcttc agatggcgtc ggcaccgcca
     9781 acgcgcatcc gccgcgttag cgcaactgca ccgccgcgag aaacagcacc atgccgccga
     9841 tcgcgccgtc gagcacgcgc cacgcgagcg cgcgcttgaa ccatggcgcg agcaatcgcg
     9901 caccgtatcc gagcgcgacg aaccataccg cgctgaccgc catcgcgccg atcgcgaacg
     9961 cgccgcgcgc gccttccggc tcgcgcgcgc cggccgtgcc gatcagcagg aacgtgtcca
    10021 gatagacgtg cgggttgagc catgtgaacg cgagcgtcat cagcacgatc ggccaggcgc
    10081 gctgcgcggg cggcgcggca accgcgcccg tcgcatcgag cgtttcgtgg ccgggcttga
    10141 acgcgcggcg cagcgcgctc aggccgaacc atgcgagata ggcgaggccg acatacagca
    10201 cggcatgaac gaaggccgga tagcgctcga cgagcaccga tgcgccgccg acgcccgcgc
    10261 cgatcaacag catgtccgac agcgtgcaga gcaggacgat cttgccgacg tgcgaacgca
    10321 tgatgccctg ccggaggatg aacgcattct gcgcgccgat ggtgacgatg agcgatgcgc
    10381 agagcatcgc gccgtgagaa aaagcgatcc agttcatgtt cgttgcggct tccggcggtg
    10441 gactaacggg gcaagaggcg ctagtctgcg atgatcgctt gagtaagaaa agctaaatga
    10501 tctaatgccg attaaggaaa actgatgctc gactatgcac tgctcgatgc gcttgccgaa
    10561 gtgatccgcc acggctcgtt cgagcgggcg gcgaaagcgc tcaacgtgtc gccttcggcg
    10621 gtgtcgcagc gcgtgaagct cttggaggag cgcgtgggca gcgtgctcgt gaagcgcggg
    10681 cagccgtgcg tcgcgacgac ctcaggcgcg ctgctgtgcc gccacaccga gcgcgtgcgg
    10741 atgctggagg ccgagctgtc cgggcacatg cccgcgttcg cgggcgcgca agcgggggcg
    10801 tggccgacgc tgcgcgtggc cgtcaacgac gacagcgtcg cgacctggtt cg
//
