LOCUS       NZ_JHEG04000001        20969 bp    DNA     linear   CON 14-MAR-2024
DEFINITION  Tolypothrix bouteillei VB521301 NODE_1_length_9494340_cov_10.278967,
            whole genome shotgun sequence.
ACCESSION   NZ_JHEG04000001
VERSION     NZ_JHEG04000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Tolypothrix bouteillei VB521301
  ORGANISM  Tolypothrix bouteillei VB521301
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales;
            Tolypothrichaceae; Tolypothrix.
REFERENCE   1  (bases 1 to 9494340)
  AUTHORS   Chandrababunaidu,M.M., Singh,D., Sen,D., Bhan,S., Das,S., Gupta,A.,
            Adhikary,S.P. and Tripathy,S.
  TITLE     Draft Genome Sequence of Tolypothrix boutellei Strain VB521301
  JOURNAL   Genome Announc 3 (1), e00001-15 (2015)
   PUBMED   25700407
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494340)
  AUTHORS   Sarangi,A.N., Mukherjee,M., Ghosh,S., Singh,D., Das,A., Kant,S.,
            Prusty,A. and Tripathy,S.
  TITLE     Improved Assembly of Tolypothrix boutellei genome
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 9494340)
  AUTHORS   Sarangi,A.N., Mukherjee,M., Ghosh,S., Singh,D., Das,A., Kant,S.,
            Prusty,A. and Tripathy,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) Computational Genomics Lab, Indian Institute
            Of Chemical Biology, 4, Raja, S. C. Mullick Road, Jadavpur, Kolkata,
            West Bengal 700032, India
REFERENCE   4  (bases 1 to 9494340)
  AUTHORS   Tripathy,S., Sanghi,N., Chandrababunaidu,M., Singh,D., Panda,A.,
            Gupta,A., Das,S. and Adhikary,S.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2014) Computational Genomics Lab, Indian Institute
            Of Chemical Biology, 4, Raja, S.C. Mullick Road, Jadavpur, Kolkata,
            West Bengal 700032, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2019
            Assembly Method                   :: SPAdes v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000760695.4-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 03:45:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,181
            CDSs (total)                      :: 8,084
            Genes (coding)                    :: 7,922
            CDSs (with protein)               :: 7,922
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 41 of 164
            Pseudo Genes (incomplete)         :: 132 of 164
            Pseudo Genes (internal stop)      :: 36 of 164
            Pseudo Genes (multiple problems)  :: 40 of 164
            CRISPR Arrays                     :: 25
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:00:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4711325
            Orig. end                         :: 4732294
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JHEG04000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20969
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="21"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20969
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20969
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10969](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10969)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(260..406)
                     /locus_tag="HC643_RS18900"
                     /old_locus_tag="DA73_0400018870"
     CDS             complement(260..406)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017740373.1"
                     /locus_tag="HC643_RS18900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018870"
                     /product="photosystem I protein PsaX"
                     /protein_id="WP_038088011.1"
                     /transl_table=11
                     /translation="MANEGKKKVGEDVAKTGATAPYPFRTGWAVLLLAINFLVAAFYFH
                     ILV"
     gene            complement(497..1399)
                     /gene="lipA"
                     /locus_tag="HC643_RS18905"
                     /old_locus_tag="DA73_0400018875"
     CDS             complement(497..1399)
                     /EC_number="2.8.1.8"
                     /GO_function="GO:0016992 - lipoate synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lipA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015113387.1"
                     /locus_tag="HC643_RS18905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018875"
                     /product="lipoyl synthase"
                     /protein_id="WP_038088014.1"
                     /transl_table=11
                     /translation="MTALEPSQIKTEIMAMPSWLRRPIGKASELSTVQRIIRQRQIHTI
                     CEEGRCPNRGECYAQQTATFLLMGPTCTRSCAFCQVDKGHAPMPVDLEEPQKVAEAVQL
                     LELRYVVLTSVARDDLPDQGAGHFVKTMETIRQMNPETQIEVLTPDFWGGVGAGEEGQR
                     DRIKTIVQAKPACYNHNIETVRRLQGPVRRGAKYDRSLFVLQVVKDIDPIIPTKSGLML
                     GHGETREEIIETMVDLRKVGCDRITIGQYMRPSLEHLPVQKYWTPEEFDELGKVAQDMG
                     FSHVRSGPLVRSSYHAGDN"
     misc_feature    complement(1464..1483)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 21.92"
                     /tool="antismash"
     misc_feature    1523..1537
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.63"
                     /tool="antismash"
     gene            1731..2369
                     /locus_tag="HC643_RS18910"
                     /old_locus_tag="DA73_0400018880"
     CDS             1731..2369
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016870052.1"
                     /locus_tag="HC643_RS18910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018880"
                     /product="response regulator"
                     /protein_id="WP_038088016.1"
                     /transl_table=11
                     /translation="MTSNKILVIDDTTVVRVKVREMLPPGNFQVLEAKDGVEGLNLIRQ
                     EKLSLIMLDFLLPKKNGWEVFQELQANSDLRKIPLVIMSGRKEEVTEKIPEPFEYFEFL
                     GKPFDQKQLIGAIKSAMTKAKQPRPEPAQVAVAATSNGVAATSNGVAATSNGVAATSNG
                     TSAVTSTESGSSAAEIQALNEKIAKMQAEIDALKKQLNQVVTFIKQKVK"
     misc_feature    complement(2753..2768)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.69"
                     /tool="antismash"
     misc_feature    2804..2817
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.84"
                     /tool="antismash"
     gene            2911..8550
                     /locus_tag="HC643_RS18915"
                     /old_locus_tag="DA73_0400018885"
     CDS             2911..8550
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.13.3"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012410185.1"
                     /locus_tag="HC643_RS18915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018885"
                     /product="ATP-binding sensor histidine kinase"
                     /protein_id="WP_038088020.1"
                     /sec_met_domain="Pkinase (E-value: 4.1e-40, bitscore:
                     136.6, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLIQLDTIFAIPGYRITQQIYSGSKTLVYQGIREKDQQSVILKLM
                     RNEYPTFHEIAQFRKQYAIAKNLNCAGIVKPYSLESYRNSYVLILEDFGGISLKEYITR
                     VETTQTAFLQQFFHIAVQIASTLHELHCHRIIHKDIKPANILINPTTYEVKLTDFSIAS
                     LLPKEIQQLTNPNFLEGTLAYISPEQTGRMNRGIDYRSDFYSLGVTFFELLTGQLPFTT
                     NDSMELVHCHIAKQPPKANSLNSNIPLTISEIISKLMAKNAEDRYHSAFGLKHDLEICK
                     QQWQETGNIIPFQLGEQDISEYFLIPEKLYGRQQEVETLLAAFERVTGGKSEIVLVAGS
                     SGIGKTAVVNEVHKPIVRQRSYFIKGKFDQFQRDIPLSGFLQAFQDLIRQLLSEPEPVL
                     QQWKSKILSALGEQAQVIVDVIPKLEVIIGRQPSVTELSGTAAQNRLNLLFQRFINVFT
                     TKEHPLVIFLDDLQWADTTSLKFIHLLSSKNTALEERTKYFNQEKQTIPEGAFLLIGAY
                     RDNEVSQMHPLSVTLNEIEKTGILINKINLKELNYNSLINLICDTLNCQEALSIPLTKI
                     VLTKTKGNPFFTHQFLKYLHDESILKFDFSHRLWQYNINEVKAIALTDDVVEFMALQLE
                     KLPSKTQAVLKLAACIGNEFDLRTLAIIYEKSEVDTAADLWQSLLDGLVLPETGYQLLV
                     DDSELSSIPNDGYQKYKFVHDRVQQAAYSLIPNNYKQSIHLKIGQLLLSSFSVEERKEK
                     IFILVNQFNVAAELLVDPSERDRLSQMNSIAGRKALASTAYVAAVKYLTTSIQLLPSDT
                     WKTNYDFTLALYETAAEAAYLSGNFELAEQLIEVVLIRAKTLLERTKVYEIKIQAVGAQ
                     SKPLPAIDVALGFLKLLGIEFPESPNQTDIQAEMAELTSNLAGRRIEDLIDYPEMTEPH
                     ALAAMRILSSVTSFAYQAIPELFPLLVFKQINLSLKYGTSPLSALVYVRYGIILCGVVG
                     DIEFGYRLGKLSLDLLVRFQVKDTISQVLMGFNGTIRLWKEHIREILPSLLEIYSISLE
                     TGSLEYGAYALYCYSFYAYFMGGELSLLKQQIENHSHALESTNQIMVLNWNNIYLQSVL
                     NLLNKQENPFELIGEAYNEKEMITYHQQANDSLGLVHLYLCKLQLCYLLADYNKAVENA
                     VNVEKYIEVAVGHLVVTEFHFYDSLAHLAVYPNAEKIEQAKILEKVSVNQEKMQKWAYH
                     APMNYLHKFYLVEAERCRVLGQYLEAVNSYEQAITLAQENEYVHEAALASELTGKFYLE
                     WGKLKIAKIYIIDAYYDYIRWGSKAKVEDLEKRYAQFLATILQQGKLNTEPATNSSSES
                     RTSLSTSGSNQTVLGSNSSISDSLDLASVVKASIALSGAIELEQLLSTLMEVVMENAGA
                     SKCALILSEGDAPSGSRPKGDRLATDVHLIVSAVSSNSTSARIDTAFPSIGLESSNDVP
                     VTLINYVKRTQEIAVIGDLKDKPVLGTDSYILREQPKSLLCIPIINQGKLLGILYLENH
                     LTAEAFTRDRIELLKLITTQAAISLENAILYQNLARVNERLEEYNRTLEEKVSERTREI
                     NEKNQHLKKALEELQNTQTQLIQSEKMSSLGQMVAGIAHEINNPINFIHGNINYATEYV
                     ESLFDLITIYQQEFSDSSTLAREKAIEIDLDFIEEDLPKLLDSMKVGTSRIRNIVLGLR
                     NFSRLDEAEMKPVDIHEGIDNTLMILHHRFKVKGTLGDRFEIEVIKEYSQLPLVECYPG
                     QLNQVFMNIIANAIDALHDCAEAQGIRLCDGYKPTIRIRTELANNDRVSVRIADNGAGI
                     PEDVSQKVFDPFFTTKSVGSGTGLGLSISYQIVVDKHKGSLTYNSIRGEGTEFVVQIPL
                     TQSHN"
     gene            complement(8647..9987)
                     /locus_tag="HC643_RS18920"
                     /old_locus_tag="DA73_0400018890"
     CDS             complement(8647..9987)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017740377.1"
                     /locus_tag="HC643_RS18920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018890"
                     /product="cytochrome P450"
                     /protein_id="WP_038088023.1"
                     /sec_met_domain="p450 (E-value: 1.1e-72, bitscore: 244.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATISQQSLPLPPGNFGLPLLGETLNFLCDPDYLEKRQKQYGFI
                     FKTNLFGHPTVVTMGADANRQLFTQEKMHLASRWPECTRALLGSGSLLIQTGNEHQNRR
                     KIFHKVFQPKIIEQSFSSIESISRSYLDKWERMGTFNWFPELSKFSLDVACKFLISLDT
                     SNDDHFSKLFEKWGDGLFSVPIPLPWTKFGRALHSRKQLLIRIEEIVRERQQQSHPGQD
                     LLGMLLQAQDEDGNKLSLQEVKEQVLTFLFGGHDTAASTLTSMCLFLAQFPEVLANARA
                     EQRQLGLEEPLTVERLKQMNYLDQVLKEVLRVNPPGAFGFRRVTKEFEVNGYRVPQDWT
                     VVYLFKRSHQDTSVYTQPECFDPGRFAPERAEDKAKPFGYLPFGGGLRECPGKEFAKLE
                     MKLLTAMLLREYEWELVSGQNLNFVQFPTPYPYDGLKVKFRRLASQH"
     gene            complement(10001..10969)
                     /locus_tag="HC643_RS18925"
                     /old_locus_tag="DA73_0400018895"
     CDS             complement(10001..10969)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017740378.1"
                     /locus_tag="HC643_RS18925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018895"
                     /product="terpene synthase"
                     /protein_id="WP_038088027.1"
                     /sec_met_domain="T1TS (E-value: 7e-76, bitscore: 255.1,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQLTFPKLHCPFPSQINKYVDVLEDSSLEWVLRFNLLAKESTYR
                     RFSKSKFFLLAARAYPNCELEELKIGNDWLSWVFIWDDQCDLSELKKQPEALKVYHKRF
                     MEILNGAEPTKNDIPLTHALHDLRQRTFQKASMQWFRHFLLCFEEYFQGCVEEAAVRAQ
                     NIVPDIDAYMMIRRCSVGGNLFLTVSEFCNNFMLSDFWRNHYFVDKLKLMTINVLAWCN
                     DIFSASREMASGDVHNIVLVLHYQQQLSLENSLDRAVIMHDREMKSLTSLEACVPVFGN
                     ELDSELTQYISIMHAWIRGNLDWYSYTDRYQSVEMLEPVKY"
     misc_feature    11575..11584
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            12084..12365
                     /locus_tag="HC643_RS18930"
                     /old_locus_tag="DA73_0400018900"
     CDS             12084..12365
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HC643_RS18930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DA73_0400018900"
                     /product="hypothetical protein"
                     /protein_id="WP_137986322.1"
                     /transl_table=11
                     /translation="MARTGITEVQILANTEIVRSRLLVCCESLFEGRRDEKSSLPSMKQ
                     ASLPFTKPLAVACGTRPLQWLLCHLPSAFFQSEVKRFDKPLQTLQSLK"
     gene            12411..14618
                     /locus_tag="HC643_RS18935"
                     /old_locus_tag="DA73_0400018905"
     CDS             12411..14618
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015111410.1"
                     /locus_tag="HC643_RS18935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018905"
                     /product="chemotaxis protein CheW"
                     /protein_id="WP_038088030.1"
                     /transl_table=11
                     /translation="MELAENDDIEAFLLESYENLDRIERDIIDLEKASSQGEALVRIYR
                     SLHTLKGNCGFLPFPKLESLAHAGESLLSALRDRLKHSPSGERKLAVTPQIVSILLQTV
                     DSIRQILSQIKTTKHEGNNDYSSLIKALTQLQETEQADTVSALPHSAVKTPILQQSKFV
                     ESSDSELPEVSALTASESSHIRVSVSLLDRVMNLVGELVLVRNQVLGFSDQFQDSAFDA
                     TCQRLNLITAQLQEGVMKTRLQPMSTIWQKFPRVIRDLALASGKQVQVEMEGADTELDK
                     SIVEAIKDPLTHVVRNCIDHGIELPAERTACGKSTIGHIFLRAFYESGKVNIEIGDDGR
                     GLDPERLKVRAQQLGLMNAIQAASASESEAINLIFLPGFSTSDRVTNLSGRGVGMDIVK
                     SNIEKINGSIEIYSQPGQGTTFKLKIPLTLTIIPALIVTCGGDRYAIPQTSLQELVCLE
                     AEQVLNSIEMLYDVPVYRLRGDLVPLVYLNQVLHIKDKVTHLETLSLVIVQAENCRFGL
                     FVDTIEDILEIVVKPLGKQLQALSLFAGATILGDGKVALIVDVVGLANRAGIITKQKHL
                     LSGDTVAENQDLVNRQQILLFQGPQGTRMGIPLTIAFRLEEIPRSAVEKVGNHYVVQSY
                     NRILPLIDLHTIFGDGEGAFASEADSLALVIVSPYSELSVGLVVDRILDIVDESLTIKG
                     IPSRPGVLLSAVIQGQITEILDIETVIRMSNPYLLQTTNRG"
     gene            14611..15054
                     /locus_tag="HC643_RS18940"
                     /old_locus_tag="DA73_0400018910"
     CDS             14611..15054
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015111411.1"
                     /locus_tag="HC643_RS18940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018910"
                     /product="chemotaxis protein CheW"
                     /protein_id="WP_038088033.1"
                     /transl_table=11
                     /translation="MAEQKLCTFFVNEIYFGIDIKYVQEVIRSQVMTHVPLAPPDICGL
                     INLRGQILTAIDLQRRLEMGESFMRSTAKFVDEVQGFNIVVCSEDEEVSLLVDEVGDVL
                     EFTTKTFQPPPATLKGKMRQMLAGAYPLEEGFVLVLDIKKILN"
     gene            15087..16391
                     /locus_tag="HC643_RS18945"
                     /old_locus_tag="DA73_0400018915"
     CDS             15087..16391
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1061: methyl-accepting
                     chemotaxis protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017313322.1"
                     /locus_tag="HC643_RS18945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018915"
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="WP_038088036.1"
                     /transl_table=11
                     /translation="MTTNRAGKTANSKSPAINSTDSVDTERSATNVQLQPLLKAMKAAR
                     DGDFSVRLPIENNGLGEIASVFNEWVSLNQSFVNQVDRLACDIGIEGKFGSQAVVKGSK
                     GSWKESLDNLNRMSSHLTAQIESINEVTLAVARGNLSEQLEEQNTGEFKQLTDNANQMI
                     GSLKFSISQMAEVATAVASSSEELTAVSQEMTENVKQTSEQATSASASAEQVNQNTSTI
                     VTAVEEMNASIREIAKTVVQGAKVATDAVKTATRTNQTIDKLGQSSVEIGKVIRVITSI
                     AQQTKLLALNATIEAARAGDAGRGFAVVANEVKELAKQTANATEDISQRIEAIQTDTKS
                     AVDAIAQITDIINQINDLQSTIASAVEEQTVTTNEIARNIAEVAKGTSDIAKNIGIVAL
                     NAQSTTDGASNMSQAAIELARMASDLQKVVNQFKY"
     gene            16407..17480
                     /locus_tag="HC643_RS18950"
                     /old_locus_tag="DA73_0400018920"
     CDS             16407..17480
                     /EC_number="3.1.1.61"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA]"
                     /GO_function="GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006482 - protein demethylation [Evidence
                     IEA]"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /GO_process="GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015129676.1"
                     /locus_tag="HC643_RS18950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018920"
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="WP_038088082.1"
                     /transl_table=11
                     /translation="MPKIRVLVVDDSVVVRSRVSKILSGDPELEVVGIAANGRIALAKI
                     PHLNPEVVLLDVEMPEMNGLETLAAIRQTYPHLAVIMFSASTRTGASATLEALSLGASD
                     YVTKPSNLGSVEATNQYIREDLIPKIKVLGARTTPLLAPMTMTHRTVPPERTRVEPVNI
                     VAIGVSTGGPNALATLLGELPSDLPVPILIVQHMPPMFTKLLAERLSSKCQIRVDEAVP
                     GVVVEPGHVWIAPGNFHMVVQRDKTVVRLATPQTPPENSCRPSVDVLLRSVAQVYGGGA
                     IAVILTGMGQDGLHGCQCIREAGGQVLAQDKASSVVWGMPGFVVSAGLADGVVPLEQMA
                     AEIMRRIRLHQVPILGT"
     gene            17495..18319
                     /locus_tag="HC643_RS18955"
                     /old_locus_tag="DA73_0400018925"
     CDS             17495..18319
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015111414.1"
                     /locus_tag="HC643_RS18955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018925"
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="WP_038088039.1"
                     /transl_table=11
                     /translation="MAVSTAEFDYLRQLVYNHSAVVLYPDKSYLAELYLQPIAEAAGFA
                     SITNLVTYLQAQPFSSLHIQAVEALVTNETSFFRDVYPFEALNHFVLPELIENRAIERS
                     LNIWCAACSNGQEPYSIAMLIREHFPMLSNWSVRLIASDFSTKVLARARQGCYNQLEIK
                     RGLPQLLREKYFYKLNSHWQIKEEISEMVEFRQINLLQSWSSLPELDIIFLRNVLIYFD
                     IATKKALLKKIKQQLKPDGFLFLGSGETIINLDKSFNRVQFDKSICYRLHNV"
     gene            18458..18823
                     /locus_tag="HC643_RS18960"
                     /old_locus_tag="DA73_0400018930"
     CDS             18458..18823
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861706.1"
                     /locus_tag="HC643_RS18960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018930"
                     /product="response regulator"
                     /protein_id="WP_038088043.1"
                     /transl_table=11
                     /translation="MALVLIIDDAAFSRRMIRKFLQGDGYEIIEATNGREGLEMVYNHK
                     PNCVLADLLMPDMNGFEFLEALQKEGLKIPTIIISADIQEGSRNQSYNLGAVNFINKPP
                     KANELREAVQQVINVKE"
     gene            18831..19436
                     /locus_tag="HC643_RS18965"
                     /old_locus_tag="DA73_0400018935"
     CDS             18831..19436
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017313326.1"
                     /locus_tag="HC643_RS18965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400018935"
                     /product="chemotaxis protein CheC"
                     /protein_id="WP_038088047.1"
                     /transl_table=11
                     /translation="MNVTADQLDALQELINIGVGRAASLLNEMIEAHICLEIPFVKVLT
                     ASQAYQELATRFEHDSLAAVRLGFTGSFYGTAGLIFPTESASTLVAVLTNEEPGSADLD
                     AVKIGTLSEIGNIVINGVMGSIGNVLRQHLNYTLPVYLEDTIDNLLLSTNVSNSQIILA
                     QARFTIKQLEIIGDIILVFEVSTFEALINVIDGEIGVA"
     misc_feature    20732..20749
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 23.88"
                     /tool="antismash"
ORIGIN
        1 aggtattggc aatccttatg gtgcgatcgc agctgctttt atcatcggta ttgctcaaga
       61 agctagcacg cctctacttg gttctcagta caaacaaggt gtcgcattat ttatcatgat
      121 tttggtgctg cttgttcgtc ccaaaggatt attcaaagga acgatataaa agggattgga
      181 gattggagat tgtaaatgag ctaatcaata cccaattgct catccccaat ttccaatccc
      241 tttcatctac ttgaacgtct tacactagaa tatgaaaata aaaggcagcg acaagaaagt
      301 taattgctaa cagtagtaca gcccaacctg tgcggaaggg gtaaggcgct gtagctccag
      361 ttttagcaac gtcttcgcct acttttttct taccttcatt agccatcgca ttctctccta
      421 atttaaagac ttattaacag ataccaaata tctgtcatat ttagcaaaat tcgttagaaa
      481 tatttgtcag acgctgttaa ttgtctcccg catgatagga actgcgaact aaaggtccgg
      541 aacgaacatg gctaaatccc atatcttgag cgactttgcc taattcatca aactcttctg
      601 gtgtccaata cttttgtact ggcaaatgtt ctaaagacgg acgcatatac tgaccgatag
      661 ttatacgatc gcatcccact ttacgcaaat ccaccatcgt ttcaataatt tcctctctcg
      721 tttccccgtg tcctagcatc aatcctgatt ttgtaggaat gatagggtca atgtctttaa
      781 ccacttgcag gacaaaaagc gagcgatcgt atttcgcccc ccgacgtact ggtccttgta
      841 aacgtcgtac tgtttcaatg ttgtgattgt aacaggctgg tttggcttgc actattgtct
      901 tgatgcgatc gcgctgacct tcctcaccag cacccacacc accccaaaaa tctggtgtca
      961 gtacttcgat ttgagtttct gggttcattt ggcgaatcgt ctccatcgtc ttcacaaagt
     1021 gacccgcccc ttggtctggt aaatcgtcac gagcaacaga agtcagcacc acgtaacgca
     1081 attctaagag ctgcactgct tctgcaacct tttgaggttc ctccaagtca acaggcattg
     1141 gtgcgtgacc tttatccact tgacaaaaag cacaagatct agtgcaggta ggccccataa
     1201 gtaaaaaagt agcagtttgt tgggcgtaac actcaccccg attcgggcag cgtccttctt
     1261 cgcaaatggt gtgaatttga cgttgcctga taatccgttg taccgttgag agttcgctag
     1321 ctttaccaat cgggcggcgc aaccatgaag gcattgccat aatttcagtt ttaatttgag
     1381 atggctctaa agcagtcata aatcattcaa aattttgcta aacgcagttg cctgaggcga
     1441 tttttgagaa aaattgtacc tctatagggg cgcaagacct ccatattcct acaataggtt
     1501 actgattcat tcgtcggtaa tttattttca gaaaatcacc taagtattac tttgccacaa
     1561 tcacagagtt aagaccgctg tttgttacgt atgacacttc aagtattgtc acttctatcg
     1621 gaaattactc tcccccgatc cagagatatg ttgggtatag tgggagaatt cttgacgccc
     1681 aagggcgcaa ctaccatata aacctaaagt ttctattaga gcaaacagtc gtgacaagta
     1741 acaaaatttt agttatcgat gacactacag ttgtcagggt aaaagtacga gaaatgttac
     1801 caccaggaaa cttccaagtt ttggaagcaa aagatggtgt agagggattg aatttaattc
     1861 gtcaggaaaa actgagcctg attatgctag atttcctact tcctaagaaa aatggctggg
     1921 aagtgtttca agagctccaa gccaatagtg atttgaggaa gattccttta gtcatcatgt
     1981 ctggtcgtaa agaagaggtg acggagaaaa tccccgaacc ttttgaatat tttgagtttc
     2041 ttggaaaacc ttttgaccaa aagcaactca ttggcgcaat caagtccgcc atgaccaagg
     2101 ctaaacaacc acgtcccgaa ccagcccagg tagcagtcgc agccacctcc aacggagtcg
     2161 cagctacctc caatggagtc gcagctactt ccaatggagt cgcagctact tctaatggaa
     2221 ccagtgcagt tacctctact gagtctgggt cttcggctgc agaaatccaa gcattaaatg
     2281 aaaaaattgc caaaatgcag gcagaaattg atgctttgaa aaaacaactc aatcaagttg
     2341 taacttttat caaacaaaaa gttaaatagc gatcggtatc gtgaggatat gtctctaacg
     2401 ctgtacttaa catagagtac ggcgtttaga tttttgttga ctaactcact gggctaaagc
     2461 cacagagatt cttttcggac atcatgcata cagacaagag cctacggtgg tcaaagcacc
     2521 atctagactg tcttcccaat tctcaaactg ggttcgagtt tacttgaacc gtttgacact
     2581 tttacagttt cctttcgggt cgtgtctgtc aatcaccgcc ttggaactac tgaattcggt
     2641 ggtacttgtg caaaattaaa tatacattgc tgaacgctat acccttgatt tagcaaagcc
     2701 aagagtgaaa gataaatata taaattaatg tatataacta ctgactggat ctcagaccac
     2761 acagtcagtt aagttctaag acagcggggt taataattat taagtttttc aaaaaaacgc
     2821 aaaaatacta ctttcggctc gtgacatgat acaaagactg tgcttctggg aatactaaat
     2881 tagaagtata ttatccagag cataatgtag atgttgatac agctagatac aatttttgct
     2941 attcctggct atcgcataac tcaacaaatt tactcgggaa gcaaaaccct ggtttatcaa
     3001 ggtattcggg aaaaagacca acaatcggtt atcttaaaac tgatgcggaa tgaataccca
     3061 acctttcatg aaattgctca gtttcgcaaa cagtatgcga tcgctaaaaa tctcaattgt
     3121 gcgggtattg tcaaacccta tagtttggaa agctatcgca acagttatgt tttgattctg
     3181 gaagactttg gcggtatttc cttaaaagaa tatatcactc gtgtagaaac tacccagaca
     3241 gcgtttctac aacaattttt tcacatagct gttcaaattg cctctaccct tcacgaatta
     3301 cactgtcatc gcatcattca taaagacatt aaacctgcta atattcttat taatcccaca
     3361 acatatgaag ttaaactgac agactttagt attgctagtc tcttaccgaa agaaattcaa
     3421 caactgacaa atcccaattt tttagaaggg actctcgctt acatttctcc agaacaaaca
     3481 ggaagaatga accgtgggat tgactaccgt agcgatttct attcccttgg cgtcacattt
     3541 tttgaactcc tgaccggaca gttacccttc acaacaaacg attccatgga gttggtgcat
     3601 tgtcatattg ccaaacaacc acccaaagct aattctttaa attctaatat tcccttaact
     3661 atatcagaga tcatcagcaa gctcatggca aaaaatgctg aagatcgcta tcacagtgct
     3721 ttcgggttaa aacacgattt agagatatgc aagcagcagt ggcaagaaac tggaaatatt
     3781 ataccctttc agttgggaga acaggatatt tccgagtatt ttttgattcc tgaaaaactc
     3841 tacggtcgtc aacaggaagt agaaacttta cttgcggctt ttgagcgagt cacgggtggt
     3901 aaatctgaaa tagttttggt tgcaggttca tctggtattg gtaagacagc tgttgtcaac
     3961 gaagttcaca aaccaattgt tcgtcagcgc agttacttta ttaaaggtaa gttcgatcaa
     4021 tttcaacgcg atattccttt atcgggattt ttacaagctt ttcaagattt aattaggcag
     4081 ttgttaagcg aacctgagcc cgttcttcaa caatggaaat ctaaaatctt atcagcatta
     4141 ggcgaacagg ctcaagtaat tgttgatgtt attcccaaac tagaagtcat tattggacgt
     4201 cagccatcag tcactgaact ctctggaact gctgctcaaa atcgtttaaa tttactattc
     4261 cagagattca ttaatgtctt cacaacaaaa gaacatcctt tggttatctt tctggatgac
     4321 ttacaatggg cagatacaac ttcgttaaaa tttattcatt tattaagcag taaaaatact
     4381 gctttggaag agcgaactaa atacttcaat caagaaaaac aaactatacc tgagggagct
     4441 tttttactta taggtgcata tcgagataat gaagtctctc aaatgcatcc cctgtctgtt
     4501 actctcaatg aaatagagaa gacaggaatt ttaataaata aaattaattt aaaagaatta
     4561 aattataaca gtttaataaa tttaatttgt gatacgctca attgtcaaga agctctatct
     4621 attcctctca caaagattgt attgacgaaa acaaaaggca atcctttttt tactcatcaa
     4681 tttctgaagt acttacatga tgaatccata ttaaaatttg atttttctca tcgtctttgg
     4741 cagtacaaca ttaatgaagt taaagccata gctcttacag atgacgttgt agaatttatg
     4801 gcacttcaac tagagaagtt gccaagcaaa actcaagcag tcttaaaact tgctgcgtgt
     4861 ataggtaacg aatttgattt aagaactctt gctattatct atgaaaaatc tgaagtcgat
     4921 acagcagcgg atttatggca atcgttactt gatggtcttg ttttacctga aactggttat
     4981 cagttattag ttgatgacag cgaactttca tcaattccta acgatggtta tcaaaagtat
     5041 aaatttgtac acgatcgggt acagcaagca gcctattctc tcattcccaa caattacaaa
     5101 cagtcaattc atttaaaaat tgggcaattg ttacttagca gtttttctgt cgaagaaaga
     5161 aaagaaaaga tttttatttt agtcaatcag tttaatgtag cagcagaact gctagtcgat
     5221 ccaagcgagc gcgatcgact gtcacaaatg aattcgatcg ctggtcgcaa agctttagca
     5281 tcaacagctt acgtagctgc tgttaaatat ttaactacca gcattcaatt actaccaagc
     5341 gatacctgga aaaccaatta tgattttact cttgctttgt atgaaacagc cgcagaagca
     5401 gcatacctaa gcggtaactt tgaactagca gagcaattga tagaagtggt attgatacga
     5461 gcaaaaaccc tattagagcg aacaaaagtt tatgaaatta agattcaggc tgttggagca
     5521 cagagtaaac cgctaccagc aatcgatgtt gcactggggt ttttaaaact tttgggcatt
     5581 gagtttccag agagccccaa ccaaacagat attcaagcag aaatggcaga acttacatca
     5641 aatttagctg gcagacgtat agaggactta atcgattacc cagagatgac ggaacctcat
     5701 gcactagctg ctatgcgtat tttatctagt gtaacgagtt ttgcctatca agctatacct
     5761 gagttgtttc ctttactcgt atttaaacaa atcaatctct cacttaagta tggcacttct
     5821 cctttgtctg cccttgttta tgttcgttac ggaataatac tttgtggagt tgtgggagac
     5881 atagagtttg gctatagatt gggcaaactc tctttagatc tgttggttag attccaggtt
     5941 aaagatacca tatcacaggt tcttatgggg tttaatggaa ctatcagact ttggaaagag
     6001 catattcgag aaatattgcc ctctctttta gaaatttatt ctattagttt agaaacagga
     6061 agtttagaat atggagctta tgctctttat tgttactcct tctatgcata ttttatgggt
     6121 ggagaacttt cactgctcaa gcagcaaata gaaaatcaca gccatgctct tgagtcaacc
     6181 aatcagataa tggtgttaaa ttggaataat atctacctgc aaagtgtctt aaatttatta
     6241 aataagcaag aaaatccatt tgagttaata ggtgaggctt ataatgaaaa agaaatgata
     6301 acatatcatc agcaagccaa tgacagcctt ggtcttgtgc atttgtattt atgcaagctt
     6361 cagctgtgtt atttactagc tgattacaat aaggcagttg aaaatgctgt taatgtcgag
     6421 aagtatatag aagtggcagt tgggcattta gttgttactg aatttcattt ctacgattct
     6481 ctggcacatc tagcagttta tcctaatgct gagaaaatag aacaagcaaa aattctggaa
     6541 aaggtatctg ttaatcaaga aaaaatgcag aaatgggcat accatgcccc tatgaattat
     6601 ttgcataaat tttatttagt agaagcagag cgatgtagag ttctgggtca atatcttgaa
     6661 gctgtaaata gttacgagca agcaattacc cttgctcaag aaaatgagta tgtacatgaa
     6721 gcggctctag ccagcgaatt gactggtaag ttttatctgg agtggggcaa acttaaaatt
     6781 gccaaaattt acataataga tgcttattat gactatatcc gttggggctc aaaagctaaa
     6841 gttgaggatt tggaaaagcg ttatgctcaa ttccttgcta caattcttca acaaggaaaa
     6901 ctcaacaccg aaccagcaac gaatagctct agtgaaagtc gcacatcctt atctacgagt
     6961 ggctccaatc aaactgtcct tgggtcaaat agcagtattt ccgattcttt agatttggca
     7021 tctgttgtaa aagcttcaat tgctctgtct ggagcaattg aactcgagca gttgctgtct
     7081 actttaatgg aagtggtgat ggagaatgct ggagcttcta agtgtgctct cattttaagt
     7141 gaaggcgatg ctccctctgg gagccgccca aagggcgatc gcttagcaac ggacgtgcat
     7201 ctgatagttt ctgcggtcag ttcaaattca acaagtgcgc ggatcgacac agcatttccc
     7261 tcaatcggtc tcgaatcaag caatgatgtt cccgtgactt taattaacta cgtcaagcga
     7321 acacaggaaa tcgcagtcat tggtgattta aaagataaac cagttttagg tactgatagc
     7381 tacattcttc gcgagcaacc caaaagtctc ttgtgtatcc cgattatcaa ccaaggtaag
     7441 ttactgggta ttctttatct agaaaatcat ttaacagcag aagcatttac acgcgatcgc
     7501 atagaacttc tcaaacttat taccactcaa gcagcaattt ctctagaaaa tgccatactc
     7561 taccagaatt tggcacgagt taacgagcga ttggaagaat acaaccgtac tttagaagaa
     7621 aaagtgtcag agagaactcg ggaaattaat gaaaaaaatc agcatctgaa gaaagcttta
     7681 gaagagttac aaaataccca aactcaatta attcaaagtg aaaaaatgtc ttctttaggg
     7741 caaatggtag cgggaattgc tcatgaaatt aataatccca ttaattttat tcatggcaat
     7801 attaattacg ctacagaata cgtagaatct ttatttgatt taattactat atatcagcaa
     7861 gaattctcag attcttccac tcttgctcgg gaaaaagcta ttgaaattga tttggatttt
     7921 atagaagaag atttgcccaa gcttttagat tccatgaagg tgggaacctc gcgtattcgg
     7981 aatattgtct taggcttacg caacttctct cgtttggatg aagccgaaat gaaacctgtt
     8041 gatattcatg agggtatcga taacactttg atgattctgc accatcgatt taaggtaaaa
     8101 ggcacgctag gcgatcgctt tgagatagaa gttatcaaag aatacagtca gctgccttta
     8161 gttgagtgct accccggcca gctcaatcag gtatttatga atattattgc caatgcgatc
     8221 gatgctctac acgattgcgc tgaagctcag ggtatacgtt tgtgcgacgg ctacaaacca
     8281 acaattcgca ttcggactga acttgcaaac aacgatcgcg tctcggttcg gattgctgac
     8341 aatggtgctg ggatacctga agatgtgagt cagaaagtct tcgatccatt ttttaccact
     8401 aaatcagttg gtagcggtac gggcttggga ttatcaatta gctatcagat tgttgtggat
     8461 aaacataaag gtagtctcac ttacaattcc atacgcggtg agggaacaga gtttgtagtc
     8521 caaattccgc taacacagtc acataactag caaataggtc agtgttaaaa attgtcgtca
     8581 tgataaggac tagtagtagt aggcgagcac tcagtaccca cctactaccg caaacattat
     8641 ttgatatcaa tgctgtgagg caagacggcg aaacttaacc ttaagaccgt cataaggata
     8701 tggtgtggga aactgaacga aatttaaatt ttgtcccgat accagttccc actcatactc
     8761 gcgtagcaac attgctgtca atagtttcat ttctaactta gcaaactcct taccggggca
     8821 ttctcgtaac ccaccgccaa aaggtaaata accaaatggt tttgctttat cctcagccct
     8881 ttctggcgca aaacgtcctg ggtcaaaaca ctccggttgt gtatagacag aagtgtcttg
     8941 atgacttctt ttaaataagt aaacgacagt ccaatcttgg ggaacgcgat aaccgttgac
     9001 ttcaaattcc ttcgtgactc tgcggaaacc aaaagcacca gggggattaa ctcgcaaaac
     9061 ctctttaaga acttggtcca gatagttcat ctgctttaaa cgttctactg ttagcggttc
     9121 ctccaacccc aactgtcgtt gttctgcacg agcattggct aaaacttccg gaaactgtgc
     9181 tagaaacaga cacattgatg tcagcgttga tgctgctgta tcgtgacctc caaatagaaa
     9241 cgttaagact tgttctttca cttcttgcaa acttaactta ttcccatctt cgtcttgcgc
     9301 ttgcaacagc atccccaaaa gatcttgacc gggatgtgat tgctgctgac gctctcggac
     9361 aatttcctca atgcgtatca gtagttgctt gcgactgtgt aatgctcgac caaacttagt
     9421 ccaaggtaaa ggtattggta ctgagaatag accatctccc cacttctcaa acaatttact
     9481 gaaatggtca tcgttagaag tatccaaact tatgagaaac ttgcaagcaa catcaagaga
     9541 gaatttgctc agttctggga accagttgaa tgtacccatg cgttcccatt tgtctaaata
     9601 actgcgggag atactttcta tactggaaaa ggactgttct atgatttttg gttggaaaac
     9661 tttatggaaa atcttgcgtc gattttggtg ctcgttacct gtttggataa gtaaagaccc
     9721 cgaccctagc aacgccctgg tacattcagg ccatctacta gcaagatgca ttttttcctg
     9781 ggtaaacaat tggcgattgg catctgctcc cattgtaaca actgtaggat gcccaaacaa
     9841 attggttttg aaaataaatc cgtattgctt ctgtcgcttt tccagataat ctggatcgca
     9901 cagaaagttt agcgtttcac caagtaacgg tagaccaaag ttaccaggag gtaaaggtag
     9961 cgattgctgg ctaatagttg ctgtcattat tttacctatc ttagtattta actggttcta
    10021 acatttcgac actttgatag cgatcggtat aagaatacca atcgagattg ccgcgtatcc
    10081 aagcatgcat tatggatata tattgcgtca attcactatc taactcgttg ccaaaaactg
    10141 gtacacaggc ttccaagctt gtcagagatt tcatctctcg atcgtgcata ataacggcgc
    10201 gatcgagaga gttttctaaa gaaagttgtt gctgatagtg cagtactaag actatgttgt
    10261 ggacatcacc acttgccatt tctctagagg ctgaaaagat atcattacac caagcaagaa
    10321 cgttaattgt cattagtttg agcttgtcta caaaataatg atttctccaa aaatcactca
    10381 gcataaagtt attgcaaaat tcactcactg tcaggaaaag atttccgcct acactgcatc
    10441 tgcgtatcat catataagcg tcaatgtcag gcacaatgtt ctgtgcgcgg actgctgctt
    10501 cttcaacaca tccttgaaag tattcttcaa agcaaaggag gaagtgacga aaccattgca
    10561 tgctcgcttt ttgaaacgtc ctctgtcgca aatcgtgtaa ggcgtgagtt agaggtatat
    10621 cattcttggt tggttccgct ccatttaaga tttccataaa tcttttgtga tacaccttta
    10681 gagcttcagg ttgttttttc aactctgaga gatcgcattg atcgtcccaa ataaataccc
    10741 aacttaacca atcgtttcca atttttaatt cttctaactc acagtttgga taggctcgtg
    10801 cagctagtaa aaagaatttt gacttagaaa accgacgata agttgattcc tttgccagta
    10861 aattgaagcg caatacccat tctaaagaag aatcttccaa aacatcaaca tatttattga
    10921 tttgagatgg aaaaggacag tgtaatttag gaaaagttaa ttgagtcata atacctctct
    10981 cagtgacaga aacgcaagac aaatagaccc aacctgaagt cagtgggctg aaatagaaac
    11041 tgtaagtggt agctaatttc tcctaactac ccctgccgat aagacttacg cactgtaggc
    11101 agaaaagagt atgtgcatta acgaatctgc aaccttaaca ggtttgcgtt caagaaaaat
    11161 ttacaaaatc ctcaccaggt tctcacactg catatgtatg cttgacatca gaattatcct
    11221 taaaaaaatg aagtcatgca tctcaaaaac agttcaaact ggaaagttga atcaatgcac
    11281 gtaaaacagg tttgatacag tgcgtaagtt ctcagcaagt ggctcatcgc gttcttacaa
    11341 agcaactcac tgctttaagt aacacgacat tttggcctca gacatatcct aatgaagatt
    11401 tacaagaagt aaatttttct tttggaagtc atgccgaaaa taaaagccat tattcttttg
    11461 aactaatact tgcaaattga cgtttcttta ggaaagcagc tagagcaaaa ctatgttctt
    11521 gccaatcaat gcaatctaat ttaaacatcg atttcctcaa gattttccat tttttgtttt
    11581 ttgaagtaac agtggttaat ccataacacg ttatacagaa attgaactta agattcagtc
    11641 tgtattttgt ggtgtttaag ataatcactg ttgcttcaaa aactttcttt tatataattt
    11701 ttgttttttt taatatttct cacatccgta cattcctatg tttaaaaatt taatcaagac
    11761 tcagtagaat agcgtatttt ctcttagaag ttataaagaa aatataaaaa aattatgaat
    11821 acaggattta ttgactgtag aaaatacgta aggtttttgt tgtcaagact aaaaacacgc
    11881 atttctctca gtattttgtt aaaattatta gtttttttca ttgtttctgg ttaagtatat
    11941 ttatttaatt gtcaggaaat agtgattatc aaagactgca aaatatattt gctttttcaa
    12001 aaattaagag aaataacttt acatggtttt ttaaaaatat agatatcttt aaaagatgag
    12061 tagagcgttg acacaataaa cacatggcta gaacaggcat tactgaggta caaatacttg
    12121 ctaatacgga aattgtgcga tcgcgcttat tggtctgttg cgagtcttta tttgaaggcc
    12181 gtcgcgatga aaaaagttca ctaccaagta tgaaacaagc gtctttacct tttactaagc
    12241 cacttgctgt agcctgtgga acccgcccac tacagtggct cctctgccat ctgccatctg
    12301 cctttttcca gtcagaagtg aagcgatttg ataaaccact acagacgctc cagagtttaa
    12361 aataaggtta ggatgaacca aaaaagataa atatagagcc tattaacgca atggagttag
    12421 cagaaaatga tgatatagaa gcatttctac ttgagagcta tgaaaatctc gatcgaattg
    12481 aacgcgacat tatcgatcta gaaaaagcgt cctctcaagg agaagcgcta gttcgcattt
    12541 atcgctcgct ccacacctta aaagggaact gtggcttttt accgtttcca aagttggaat
    12601 cactagccca tgcgggagag agtttgttaa gtgcgctacg cgatcgtcta aagcactcgc
    12661 cctctggcga gcgcaagcta gcggtaactc ctcaaatcgt tagtatctta ctccagacag
    12721 ttgacagcat ccggcagatt ctttctcaaa taaagacgac caagcatgag ggcaacaacg
    12781 attactcatc actcatcaag gctttaactc aattgcagga aaccgaacaa gcagacacag
    12841 tgtctgcatt acctcactct gctgttaaga ctccaatact acagcaaagt aagtttgttg
    12901 agtcgagtga tagcgaactt ccagaagtgt cagccctaac tgcatcagaa tcttctcaca
    12961 tccgagtcag cgtttctcta ttagatcggg tgatgaactt agtcggagaa ctcgttttag
    13021 tccgcaacca agtcctcgga ttttcagacc agtttcagga tagtgccttt gatgcgactt
    13081 gccagcgcct taacctaatt acagcccagt tgcaggaagg ggtgatgaaa actcgtctac
    13141 agccaatgag taccatttgg caaaaattcc ctcgcgtgat tcgcgatttg gcactcgcct
    13201 ccgggaaaca agttcaggta gaaatggaag gagcagatac ggaactcgat aaaagtattg
    13261 tagaagccat caaggaccct ttgactcacg tagtccgtaa ctgcatcgat catggtattg
    13321 agttaccagc agagcggacc gcttgtggta aatcaaccat agggcacata tttttgagag
    13381 ctttttatga aagcggtaaa gtcaatattg agatcggcga tgacggtcgc ggtctcgacc
    13441 cagaacgact caaagtgcga gcgcagcagc ttggtttaat gaatgccata caggctgcga
    13501 gcgcaagcga gtcggaagcg ataaacttaa tttttttacc aggcttctcg actagcgatc
    13561 gggtaaccaa cctttctggg cgaggggttg gtatggatat tgttaagagt aatattgaga
    13621 aaattaacgg cagcattgaa atttacagtc aaccgggaca aggaacgacg tttaaactga
    13681 agattccatt gaccttgaca attatcccag cattaattgt cacctgtggg ggcgatcgct
    13741 atgctattcc ccaaacgagc ctgcaagagt tagtttgctt agaagcagaa caggtactta
    13801 atagtatcga gatgctttac gacgttcccg tgtatcgctt gcggggcgat cttgtaccac
    13861 tggtttactt aaatcaagtg ttgcacatca aagacaaggt tactcattta gaaacactga
    13921 gtctggtaat tgtccaagct gagaactgtc gatttggtct gttcgtagac accattgaag
    13981 atattctaga gattgtcgtt aaacctctag gaaagcagtt gcaggcacta tccctatttg
    14041 caggcgctac tatccttggc gatggcaaag tagcattgat tgtagatgtc gtcggtttgg
    14101 caaaccgggc tggtataatc acgaaacaaa agcatctgtt gtcgggcgat acagtcgcag
    14161 agaaccaaga tctggtgaat cgccaacaga ttttactatt tcagggtccc caaggtacac
    14221 gcatgggtat tcccctaaca atagcatttc ggcttgaaga aattcctcgt tccgctgtgg
    14281 agaaagtcgg aaatcattat gtggtgcagt cttacaatag aattttgcca ctgatcgatc
    14341 tgcatactat atttggtgat ggcgaaggag cttttgcaag tgaagcagat tcgcttgcgc
    14401 tcgtgattgt ctctccctac tcagaactga gtgtcgggct agtggtcgat cgcattcttg
    14461 atatagtaga cgaatcatta acaatcaaag gtattcctag cagaccgggc gttcttttga
    14521 gtgctgttat tcaaggtcaa attacagaaa ttcttgacat tgaaactgtg attcggatgt
    14581 ccaaccccta tttactgcaa acaaccaatc gtggctgaac aaaaactttg cacttttttt
    14641 gtcaatgaaa tttactttgg cattgatata aagtatgttc aagaggtgat tcgctcccaa
    14701 gtgatgactc acgtaccttt agcaccacct gatatttgtg ggctcattaa cctgcgagga
    14761 caaattttga cggcgatcga tttgcagagg cgattggaaa tgggcgagtc gttcatgcgc
    14821 tcgaccgcta aatttgtaga tgaagttcag ggatttaata ttgttgtatg ttcggaggat
    14881 gaagaagtta gcttgctcgt tgatgaagtt ggagatgtct tggaatttac aacaaaaacc
    14941 tttcaacctc cacccgcaac gctaaaaggt aaaatgcgtc aaatgctagc aggagcttac
    15001 ccactcgaag aggggtttgt gctagttcta gacatcaaaa aaattttaaa ttaacattta
    15061 aaatcagcat aaaggagcgc ttggtcatga ctacaaatcg ggctggcaaa acagccaact
    15121 ctaaatcgcc tgcgataaat agtacggatt cggtagatac cgaacgcagt gcaacaaatg
    15181 tacaactgca accgttacta aaagccatga aagcagcccg agacggtgat tttagcgtgc
    15241 gtttaccaat cgagaataat ggtttgggtg aaattgcgtc ggtttttaat gaatgggtga
    15301 gtttgaatca aagttttgtc aaccaagtcg atcgcttggc atgcgatatc ggtatagaag
    15361 ggaagtttgg ttctcaggct gttgtcaaag gatcgaaagg ttcttggaaa gaatctcttg
    15421 acaacttgaa ccggatgtcg tcacatctaa cagcgcagat tgaaagtata aatgaagtca
    15481 cgcttgctgt tgctcgagga aatttgtccg agcagcttga agaacagaat actggagagt
    15541 ttaagcaact tactgataat gccaatcaaa tgattggcag cttgaaattt tccatcagtc
    15601 aaatggcaga ggttgcaacc gcagttgctt cttcctcaga agaactaacc gcagtcagtc
    15661 aggaaatgac agagaacgtc aaacaaacat cagaacaggc aacttccgcg tctgcctcag
    15721 cagagcaggt aaatcaaaat accagtacaa tcgtgactgc ggtagaagag atgaatgcca
    15781 gcattcgaga aattgctaaa actgtcgtcc aaggagcaaa ggtcgcaact gatgccgtaa
    15841 aaaccgccac tcgcacaaac cagacaattg acaaactcgg tcaaagtagt gttgaaattg
    15901 gcaaagtcat tagggtaatt acttcgatcg cccaacaaac aaagttactc gcactcaatg
    15961 ctacaattga agcggcaaga gcgggggatg caggtagggg ttttgctgtc gtcgccaacg
    16021 aggtgaaaga actagccaaa caaacggcaa atgctaccga agatatcagt cagcgaattg
    16081 aagcaattca gactgatacg aaaagtgctg ttgatgcgat cgcgcaaatt acggacatta
    16141 tcaaccagat taacgacctg caaagcacga tcgcgagtgc tgttgaggag caaacagtta
    16201 ctacgaatga aattgctcgc aatattgccg aagttgccaa aggaacatcg gatattgcca
    16261 aaaatattgg aattgtggcg ctcaacgctc aaagcacgac agatggagcc agcaatatgt
    16321 cacaagcagc catagagcta gctcgtatgg catcagattt acagaaagtt gtcaaccaat
    16381 ttaagtatta gttggttttt atcattatgc ccaagattcg ggtgctggtt gtggatgact
    16441 cagtggttgt tcgctctcga gtgagtaaga ttttgtctgg cgatccagaa ctagaagtcg
    16501 tagggattgc agccaatggt cgtattgccc tagctaagat tccccatctc aatccggaag
    16561 ttgtgctttt ggatgtcgag atgccagaga tgaatggttt agaaaccctt gctgcaatcc
    16621 gacaaactta tccacattta gcggtgatta tgttcagcgc ctccacacgt actggagcaa
    16681 gcgcgacact tgaagctctg tctttaggtg catcggatta cgtaacaaaa ccaagtaatt
    16741 taggaagtgt ggaggcaaca aaccaatata tccgtgagga cttaattcct aaaatcaaag
    16801 tacttggtgc cagaacgacg cccttactcg cgccaatgac aatgacccat cgaactgttc
    16861 ctcctgaacg aactcgggta gagccagtta atattgttgc aattggggtt tctactggag
    16921 ggcctaatgc tctggcgaca ctgcttggag agcttccatc cgatctacca gttcctatcc
    16981 tgatcgtgca acatatgccg ccgatgttta caaaactatt agcggaacgg ttatcctcta
    17041 agtgtcaaat ccgagtggat gaagcggttc ctggagtcgt agtagaaccc ggacacgtgt
    17101 ggatcgcacc gggaaatttt cacatggttg tgcaacgcga caaaactgta gtgcgacttg
    17161 ccacccctca gacacccccc gaaaattcct gtcgcccttc ggttgatgtc ctcttgcgtt
    17221 ccgttgcaca agtctatggt ggtggcgcga tcgcggttat actaacgggg atgggacaag
    17281 atggattgca cggctgccaa tgtatccgcg aagcaggagg acaggtgctt gcacaagaca
    17341 aagcaagtag cgtggtgtgg ggaatgcccg gtttcgttgt cagtgcaggg cttgccgatg
    17401 gggttgttcc tctcgagcaa atggcggctg aaatcatgcg ccgaattcgt ctccatcaag
    17461 ttcctatttt aggtacgtag taataaagta ggctatggct gtaagtaccg ctgaatttga
    17521 ctatcttcgt caattagtat ataatcactc ggcagttgta ttatatcctg ataaaagcta
    17581 tttagcagag ctatatttgc agccaattgc agaagcggca ggatttgctt caattaccaa
    17641 tttagtcact tacctgcagg ctcagccttt cagcagcctt cacatccaag cggtagaggc
    17701 actcgtcacc aacgaaacct cattttttcg tgatgtttat ccctttgaag cactgaacca
    17761 ttttgtgttg ccagaattga tcgaaaaccg agccattgag cgatcgctaa atatttggtg
    17821 tgctgcttgt tccaacgggc aggaacctta tagtattgcc atgctcattc gcgaacattt
    17881 tcccatgctg agcaattggt ctgtgcggtt aattgctagc gatttttcca ccaaggtact
    17941 agcacgagcc cgtcaaggat gttacaacca gcttgaaatc aagcgcgggc tacctcaact
    18001 cctgcgcgag aaatattttt ataagcttaa cagtcactgg caaattaagg aagaaatctc
    18061 tgagatggtt gagttccgtc agatcaatct cctgcaatcg tggtcatccc tccctgaatt
    18121 agacattata tttttacgca atgttttaat ctattttgat atagcaacta agaaagcatt
    18181 actgaaaaag ataaagcaac aattaaaacc agacggattc ctatttctcg gtagtggtga
    18241 aacgattatt aacttggata aatccttcaa ccgcgttcaa tttgataaga gtatttgtta
    18301 ccgattacac aatgtgtaga gcggcacaag taacgattct acaccagacc tatgcctaac
    18361 ggaatagaac cgcgaacgag ttggtataag gcttctagcg agctagctaa catataatca
    18421 atgataataa ttgaaaaaat caataagggc aaagctgatg gcattggttc taattatcga
    18481 cgatgcagcg ttttctcgca gaatgatccg caagtttttg caaggtgatg gctatgaaat
    18541 cattgaagca actaatggac gtgaaggatt agaaatggtt tacaaccaca agcccaactg
    18601 cgtattagca gatcttttaa tgccagatat gaatggattt gaatttcttg aggctttaca
    18661 aaaagaagga ttaaaaatcc ccacaattat catctcagct gatatccaag aagggtcgcg
    18721 caatcaaagt tataatttag gggcagttaa ctttattaac aaaccgccca aagcaaatga
    18781 attgcgagag gcggttcagc aagttattaa tgtgaaggaa taaagctccc atgaatgtga
    18841 cagcagatca actagatgcc ttacaagaat tgattaatat tggcgttggt cgggcagcta
    18901 gtctactcaa tgagatgata gaggctcaca tttgtttgga aattccgttt gtaaaagttt
    18961 tgactgcttc acaagcctat caagaattgg caacacgatt cgagcatgat agtttggcgg
    19021 ctgtaagact gggttttact ggctcgtttt atggtacggc tggcttaatt tttccaactg
    19081 aaagcgcatc aacattggtt gcagtcctga ctaatgaaga accaggttcg gctgacttag
    19141 atgccgtcaa aattggtacg ctgagcgaaa ttggcaatat tgtgattaat ggggtgatgg
    19201 gttccatcgg caatgtactc aggcagcacc tgaattacac gttgcctgtt tacttagaag
    19261 atacgattga taatttattg ttatctacaa atgtaagtaa ctcacaaatt atacttgcac
    19321 aagcacgctt cacaattaaa caattagaaa ttattggaga tattatttta gtttttgagg
    19381 tcagtacatt tgaggcgtta attaatgtga ttgatgggga aattggagta gcttaaacac
    19441 agaagaaata ttaaattttg gagcagtaga atattaaaat cggtctgatg aaatgagaaa
    19501 agaaattgag caagcacagg aaaagtttag ccttttagat cgaattcctt tgggaaattt
    19561 tatattgcac tcagatcgca cagtgttatt ttggaactgc tgcttagagg aatggacaaa
    19621 aattccaaga agtaagattt taggaaattc aatttatgaa tattttcctc atctaaatca
    19681 acctcgttac gccagtcgct tgcaccaaat ttttgaaggt ggacctccta caatcttttc
    19741 ttctcaattg cataaatatg tcattcccgt gcctgtagca caggataaat atcgcattca
    19801 acacacaaca gtaacagctg tacctgcatt gttcggagat gatttttatg ctcttttctc
    19861 aattcaagat gtcacagatt taacgtttcg agttcaggaa tataggaact tgcgagacca
    19921 agcattggct gtagcggaag aacgccaacg ggcaaaagaa gtggcagaaa cagcaaaccg
    19981 catcaaagat gaatttctcg ccatagtttc tcacgaactt cgttctcctc tgaatcctat
    20041 tttgggttgg gcaaagctcc ttaaaaaacg ttcgttgaat gaggttgcta gcttgcgtgc
    20101 tattgaaacc atcgaacgca atgccgagct acaggtgcaa ttgattgacg atctgttaga
    20161 tatttctcgc attcttcggg gcaaactcgc acttaattta gaaatcgtga atcttacttc
    20221 tattattgaa gcggctttag agacagtgcg attggcagca gaagcaaaat caattcaaat
    20281 gaatttgaat ttagacgcaa aaattggacg ggtaaagggt gatagcaatc gtcttcaaca
    20341 agttatttgg aatctgcttt ctaacgcagt caaattcaca cccattggtg gcgaggtaga
    20401 agtctactta gaacaaattg gttctgaggt acaaatcagc gtcagggata caggtcaggg
    20461 tattagttct gagtttttgc cacacgtatt tgagtatttc cgtcaagccg atagtagcat
    20521 cacccgcagg tcaggtggac tggggctggg tttggcaatt gtacgcaagc ttgtcgagtt
    20581 acatggtggt agagtttggg cagagagctt tggcgaagga caaggcgcga catttacagt
    20641 ttgtttacca gcgctgcaac aaggtcagga gttggtacgg aaagataaca acaaggttct
    20701 taacaattcg tatcccttct caatgctctg caatccctta gaagggatac gagtgttagt
    20761 tgtggatgat gaggttgata cacgcgagtt tctagccttt ctattggaac agcagggagc
    20821 aattgtcaca acagcgacat cagcccgtga agcacttgct gaaatagcaa ggtcaaaacc
    20881 agatttgctg ttgagcgata taggtatgcc agaagttgat ggttattctt tgatccagaa
    20941 actgcgatcg cttagcgcca gtcaaggag
//
