LOCUS       NZ_JHEG04000001        20546 bp    DNA     linear   CON 14-MAR-2024
DEFINITION  Tolypothrix bouteillei VB521301 NODE_1_length_9494340_cov_10.278967,
            whole genome shotgun sequence.
ACCESSION   NZ_JHEG04000001
VERSION     NZ_JHEG04000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Tolypothrix bouteillei VB521301
  ORGANISM  Tolypothrix bouteillei VB521301
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales;
            Tolypothrichaceae; Tolypothrix.
REFERENCE   1  (bases 1 to 9494340)
  AUTHORS   Chandrababunaidu,M.M., Singh,D., Sen,D., Bhan,S., Das,S., Gupta,A.,
            Adhikary,S.P. and Tripathy,S.
  TITLE     Draft Genome Sequence of Tolypothrix boutellei Strain VB521301
  JOURNAL   Genome Announc 3 (1), e00001-15 (2015)
   PUBMED   25700407
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494340)
  AUTHORS   Sarangi,A.N., Mukherjee,M., Ghosh,S., Singh,D., Das,A., Kant,S.,
            Prusty,A. and Tripathy,S.
  TITLE     Improved Assembly of Tolypothrix boutellei genome
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 9494340)
  AUTHORS   Sarangi,A.N., Mukherjee,M., Ghosh,S., Singh,D., Das,A., Kant,S.,
            Prusty,A. and Tripathy,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) Computational Genomics Lab, Indian Institute
            Of Chemical Biology, 4, Raja, S. C. Mullick Road, Jadavpur, Kolkata,
            West Bengal 700032, India
REFERENCE   4  (bases 1 to 9494340)
  AUTHORS   Tripathy,S., Sanghi,N., Chandrababunaidu,M., Singh,D., Panda,A.,
            Gupta,A., Das,S. and Adhikary,S.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2014) Computational Genomics Lab, Indian Institute
            Of Chemical Biology, 4, Raja, S.C. Mullick Road, Jadavpur, Kolkata,
            West Bengal 700032, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2019
            Assembly Method                   :: SPAdes v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000760695.4-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 03:45:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,181
            CDSs (total)                      :: 8,084
            Genes (coding)                    :: 7,922
            CDSs (with protein)               :: 7,922
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 41 of 164
            Pseudo Genes (incomplete)         :: 132 of 164
            Pseudo Genes (internal stop)      :: 36 of 164
            Pseudo Genes (multiple problems)  :: 40 of 164
            CRISPR Arrays                     :: 25
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:00:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2795382
            Orig. end                         :: 2815928
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JHEG04000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20546
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phenazine"
                     /region_number="12"
                     /rules="phzB"
                     /tool="antismash"
     cand_cluster    1..20546
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="phzB"
                     /kind="single"
                     /product="phenazine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20546
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:10546](+)"
                     /cutoff="20000"
                     /detection_rule="phzB"
                     /neighbourhood="10000"
                     /product="phenazine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10546
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="phzB"
                     /neighbourhood="10000"
                     /product="phenazine"
                     /protocluster_number="1"
     misc_feature    complement(260..276)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 23.43"
                     /tool="antismash"
     misc_feature    complement(312..326)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 21.98"
                     /tool="antismash"
     misc_feature    complement(555..569)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.51"
                     /tool="antismash"
     gene            675..1142
                     /locus_tag="HC643_RS11135"
                     /old_locus_tag="DA73_0400011130"
     CDS             675..1142
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016862423.1"
                     /locus_tag="HC643_RS11135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011130"
                     /product="DUF3574 domain-containing protein"
                     /protein_id="WP_038072532.1"
                     /transl_table=11
                     /translation="MKFKISYNTYGFILGIGLIFNCALAISIYQKSSDIETYINSFCKN
                     QLNGKSFTRTELLFGLSKSDGSRVNEKEFQFFIDNEVTPLFPDGLTLLTGKGQFKNSRK
                     IVVKEGSKLLILLYPFNSDSNRKIQQIRQAYVKNFQQESVLRVDELSCVSF"
     gene            1366..3786
                     /locus_tag="HC643_RS11140"
                     /old_locus_tag="DA73_0400011135"
     CDS             1366..3786
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745400.1"
                     /locus_tag="HC643_RS11140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011135"
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="WP_038072534.1"
                     /transl_table=11
                     /translation="MPQYFGKLPTTDQPWTTIGRVQSVNKSDAAKQPLRERVIHLECGE
                     ARVSISVLASNLLRVRMSPTGEFQPRRPWAVTLDDTEWTNVPFEVNVTDSVVEIETEQI
                     RVSVQREKCRITCFDKEGRPFAQDTEMAMGWRLGAVAAWKHIEAEEHFYGFGERTGLLD
                     KLSEVKTNWTVDALDYSTQTDEMYQAIPFFIALRPQLAYGIFFNTTFWSQFDIGAEAPG
                     TLKMETRGSELDYYIIYGPEPADILRTYTQLTGRMALPPKWSLGYHQCRWSYESETIVR
                     ELAQEFRSRQIPCDVIHLDIDYMRGYRVFTWSPNRFSDPAKLIGDLGQNGFKTVTIIDP
                     GVKYEPEANYHVFDQGIKHDYFVRKVDGQLFHGYVWPEKSVFPDFLRSDVRHWWGDLQK
                     SLTDAGVAGIWNDMNEPAIDDRPFGDGGNKIWFPLDAPQGGDKEDKGDKGEGSTVSPLS
                     TLLSHRATHAEVHNLYGLSMARASAEGLQRLRPNERSFVLTRSGFAGVQRWSSVWMGDN
                     QSLWDHLEMSLPMLCNMGLSGVAFVGCDIGGFAGNATAEMFARWMQVGMLYPFMRGHSA
                     MSTARHEPWVFGERTEKICREYINLRYQLLPYLYTLFWEAATTGTPILRPLLYHYPNDP
                     KTYTLYDQVLLGSHLMAAPVYRPGVEHRSVYLPEGTWYDWWTGESYHGPVHILAHAPLE
                     TMPLYVRAGAIIPMQPVMQYVDERPLNQLRLRIWHGASEFTLYEDDGHTFEFQKGAFAT
                     TNYRVFANAEHTILEIEERQGEWIPPSREVIVELVGVGKQQFQDDGKPRTLKF"
     misc_feature    complement(3892..3905)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 23.02"
                     /tool="antismash"
     misc_feature    4078..4091
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.31"
                     /tool="antismash"
     gene            4118..6889
                     /locus_tag="HC643_RS11145"
                     /old_locus_tag="DA73_0400011140"
     CDS             4118..6889
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1061: methyl-accepting
                     chemotaxis protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745401.1"
                     /locus_tag="HC643_RS11145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011140"
                     /product="MHYT domain-containing protein"
                     /protein_id="WP_038072536.1"
                     /transl_table=11
                     /translation="MLSSTYDQGLVALSIVLAVLSSYTALDLAGRVSAVEKRVRLAWLI
                     GGAITMGIGIWSMHFVAMLAFSLPIPIVYDVGIVVFSITPAIATSAGALFLASRRFMSM
                     KRLLTGGVLMGLGIASMHYIGMAAMRMDASTHYDILLFVLSVAIAIGASIAALYIAFQF
                     RIQTSKTGRSSRIFSAIVMGAAISGMHYTGMAAVSFTPTKVVSSVLANRVVQSSLTWLA
                     IGIGMATLVVLSVTLLTSFADRRITSQTTLLKQQEVEAQRLQQFTEMTLRIRRSLKLED
                     VLNTAVSEVQQVLGADRAIIRRLNRDWSGTIIAESVAQGWISTLGHTMDDSFWERYLES
                     YNNGRVWVIDNIYQVGFAEEHLAILKHFQIKAYIAAPILQNDRLQGLLFAHQCSNARIW
                     QKWEIELFRQLAVQIGIALEQASLLHELQQAQEVLRLRERAIAAASNGILITAPLQEDN
                     PIVFCNAAFETMTGYSLEEVLGRNCRFLQGEGTNPATVREINNAVKQERDCQVVIKNYR
                     KDGTPFWNQLAISPVRNASGQVINFIGVQADITEQMQVQEQLRLSKENLQNQVVELLSD
                     VEEASRGDLTVHAQMSKGEIGIVANFFNAIIESLRHIVIQVKQAVQQVNVLVGENSQAT
                     RLLADDALKQADEITRTLKSLDEMVLSIQAVADSAHKAAVMARTASTTAEVGRDSMERT
                     VDSITNLHLTMAEAAKKVKQLGESSQKISKVVSLINQIALQTNVISINASIEAAKAGEE
                     GQAFAVVAEEVGDLAAHSAQATEEIQQIVRNIQIETSEVVKAVEQGMTQVVEGTDSVKD
                     TKQQLEKILEVSREIDLLVQSISHATVSQAQTSQEVASLMKQIAQDSLHTSDFSRLMSS
                     SLEKTVDVAQQLQASVTVFKTDPEERLSVEPLEAADIAQSSGRDSVVLKMTQ"
     gene            complement(6984..7628)
                     /locus_tag="HC643_RS11150"
                     /old_locus_tag="DA73_0400011145"
     CDS             complement(6984..7628)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745402.1"
                     /locus_tag="HC643_RS11150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011145"
                     /product="SH3 domain-containing protein"
                     /protein_id="WP_038072538.1"
                     /transl_table=11
                     /translation="MASIFYVATKEGDPLTVRSKPNGESVGSLSNGTEVQVTGEPVQAG
                     NRKWVQIGTNRWVANEFLTSIKTARVVAKRTTKTIGGGLRVYETRLIDSNGNVINTVQA
                     VSGRVGKQTPSDVAGSETPVPFGIYKFDNPGVVGTPPGNKPEFGGVWSPVTPLFSTGRS
                     GIGVHYDPSALAQNANTGTAGCFATPTVKERDIMTNFIRTHKPTHLIVYEG"
     gene            7928..8779
                     /locus_tag="HC643_RS11155"
                     /old_locus_tag="DA73_0400011150"
     CDS             7928..8779
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015120664.1"
                     /locus_tag="HC643_RS11155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011150"
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_038072540.1"
                     /transl_table=11
                     /translation="MQQPKPQQTAAILKAEWNDLIVEYGRLQEVGDFDFAMPKYAISVA
                     FLPHERVTWSVDGSKPQTSALPAGSVFLYSDREFVWHRREKESEYVNLKIEPVLLNRLA
                     NESGLSTPVRLEHRVLFPDPTILHVAHLLKSELVNSGFAGNLYVESLRNLLAVHLLRNY
                     GGVLSHSTPEEIGVLDVLKLKHIKDYIEDNLASELAIATLAALVPMSQFHFARAFKTLV
                     GEPPHRYILQRRIERAKVLLKVTRFSSAEIAYQVGFSNPSHFTAQFRKLTGVTPKQFRD
                     SA"
     gene            8884..9942
                     /locus_tag="HC643_RS11160"
                     /old_locus_tag="DA73_0400011155"
     CDS             8884..9942
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316611.1"
                     /locus_tag="HC643_RS11160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011155"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_237265865.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.3e-06, bitscore:
                     27.2, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTMLSAIKASALSLVLLTSSAYAHSQNLSESKNSTQPQVQQLSD
                     TVIAANAYKPGVNRVTFQSEGETLVGNLYLPASYKAGDKLPAAIVAGSWTTVKEQMPSI
                     YARKLAEQGFAALAFDFRTFGESGGKIRSFESPTAKIVDIKNAVSFLQTVDAVDRNRIA
                     GLGVCASAGYMAVATATDSRIKSFVAVAPWIHDAAIVNTIYGGEKAVREKIKIAQAART
                     KLQQTGQVDYVPAASATNGAAAMYGNIDYYLNPKRGAIPQWANKFAVMSWAEWLTFSAM
                     PQAKQIKVPTLFIHSEKAAIPDGARRFFAAIPGNNKNMVWFPETQQFDFYDREATINQA
                     VALTVKQLRTSL"
     gene            10001..10546
                     /locus_tag="HC643_RS11165"
                     /old_locus_tag="DA73_0400011160"
     CDS             10001..10546
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phenazine: phzB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015255.4"
                     /locus_tag="HC643_RS11165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011160"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_050045677.1"
                     /sec_met_domain="phzB (E-value: 2.7e-29, bitscore: 101.0,
                     seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLMQKALIGATLMLSLLTIGPQTQAGITTHIATANTNAEAIRQNN
                     EQTIRTYFQLLSQKRMDEWLKLWADDGVQDMPYSPAGFPKRVEGKAAVAKHYSALPTSV
                     GRMVFPDLKIYPTTDPNTFFVEFRGEIEVLATKKPYNNTYAGLFQFHNGKVVLFREYYN
                     PIVFVEAWGNPSGFTQQK"
     gene            10625..11737
                     /locus_tag="HC643_RS11170"
                     /old_locus_tag="DA73_0400011165"
     CDS             10625..11737
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877047.1"
                     /locus_tag="HC643_RS11170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011165"
                     /product="aldo/keto reductase"
                     /protein_id="WP_038072542.1"
                     /transl_table=11
                     /translation="MSLNTYYTLGRSGLRVTRLALGAMTFGTEWGWGSDEATARQMFNT
                     YVDAGGNFIDTADLYTNGTSETWLGKFIAERNLRDRIVVATKFSYNAEPGNPNAGGNGR
                     KNIMRAVEGSLQRLGTDYIDLYILHTWDTITPAEEVIRTLDDLVRSGKVRHIGLSNTPS
                     WYAARAQTIAEWRNYEPLSALQLEYSLIERNIEREFIPLGLELGMGTMVWSPLASGLLS
                     GKYRPSEGGFTGEGRLETVRGISNPAFQKFSDRNWKIVAELETVAKELGRSMAQVAVNW
                     TANRPGIASVIIGATKQSQLEDNLKALSFEIPTELANRLEAISRPEPQFPYIFFGSEIQ
                     GMIHGGATVGYKPAGYQPNVLIQSQGAGVS"
     gene            11777..12349
                     /locus_tag="HC643_RS11175"
                     /old_locus_tag="DA73_0400011170"
     CDS             11777..12349
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /locus_tag="HC643_RS11175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011170"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_050045678.1"
                     /transl_table=11
                     /translation="MNHQRRNVLLTFSASLIGFLSTTEIAKARTTSPTQVNSQVPMNTI
                     ILQKLVDRAEIIDAINIIGMGADLRDWKACRGAFADRVLTDYTSLNGGTPNTVAADTLV
                     AGWADFFSKTFKATQHMITNHAVIVNGERANCTSHFRAHHVYLEGKPGKTWTLGGFYNH
                     ELSHINGRWKVTQMKMTWTYEEGTRPG"
     gene            12354..12803
                     /locus_tag="HC643_RS11180"
                     /old_locus_tag="DA73_0400011175"
     CDS             12354..12803
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HC643_RS11180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DA73_0400011175"
                     /product="hypothetical protein"
                     /protein_id="WP_038072544.1"
                     /transl_table=11
                     /translation="MMQSDILVLQASWDELAIKYAYLVKPPVSSSNTLIVSIVTYQIEV
                     TAAIPTGGFVNAEEIESDWLNDVQKKTLLDYLQITLSLLRFEEIVDQKMLCADRQPILK
                     RQITNPSILHLAHLLRAEMEAPKFFSQQFVSSIIKALIIHCWYQL"
     gene            complement(12848..13609)
                     /locus_tag="HC643_RS11185"
                     /old_locus_tag="DA73_0400011180"
     CDS             complement(12848..13609)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459314.1"
                     /locus_tag="HC643_RS11185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011180"
                     /product="hypothetical protein"
                     /protein_id="WP_038072546.1"
                     /transl_table=11
                     /translation="MNLSTYFMHLVGSGISAYYFAAQIRDPKTRPNVLAGFQLAESGSV
                     PFLTALSDRATAEGDTWLAEKLAQHASDETKHGQIFAHALKQLNKEVIDFKNLPKNTQE
                     NKSENKSQERRSPFFAAFFEGYSQEQLKPEVIDWDVFMGSTYILELDASKDFTRMANVL
                     PENDPVTRNLKLGILSIAKDETGHAAYIYEAMMRRMPSANVERLVDEWRTRKTNAMLAF
                     AGNMLQRKNNSRSMVQDAATTSETHSEVVAA"
     misc_feature    13693..13704
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.92"
                     /tool="antismash"
     misc_feature    13804..13820
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.35"
                     /tool="antismash"
     misc_feature    complement(13806..13819)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.16"
                     /tool="antismash"
     gene            14071..15864
                     /locus_tag="HC643_RS11190"
                     /old_locus_tag="DA73_0400011185"
     CDS             14071..15864
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453747.1"
                     /locus_tag="HC643_RS11190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011185"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_038072548.1"
                     /transl_table=11
                     /translation="MTDRVYAADVLVVGGGTGGTAAALQAARRGVKTILVSEFPWLGGM
                     LTSAGVSAPDGNELEAFQTGLWGAFIKQLQRRQPMGLDNSWVSFFSYDPRIGAEIFADW
                     VQELPNLLWLPGRRPLEVIQQNDCVFGVRFADFTVTAKVTLDATELGDLLDLAEVPYRW
                     GWELRSEWGEISAPASFNYLTEKYPVQAPTWVVVMQDFGESMAPEIPSAPNDNPSQFTG
                     AWDNCGAEQFLNYGRLPGGLFMINWPIHGNDYGHGVGRVIASSEKRREFLQECLWHSQN
                     FARFIQHQIGYRYGLAHHIFPPFPRSPIPSISPSSFALHPYYRESRRLVGLTTIREQDI
                     LPIAGGSVAPLHEDAIAIGNYANDHHYPEIKFELLPKSIRWGGRWTGTPFTIPYRCLIP
                     IKTDGLLVCEKNISVSHIANGATRLQPVVMNIGQAAGMAAALCIELNLSPRDLPVRTLQ
                     EALLRDRHHAAAIVPIFNLPPDSPDWLHMQLYYLDNPETYPHSGNCPHFSPTVGFYSYG
                     DAVNLRNIDCFMGFFQCQTQQDYKFIITSPSDYQGQTWQIVTLRSDIDEQLQTYLHGQF
                     IKILGRFNSAGNWLLAEHIEC"
     gene            complement(16728..17786)
                     /locus_tag="HC643_RS11195"
                     /old_locus_tag="DA73_0400011190"
     CDS             complement(16728..17786)
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017747010.1"
                     /locus_tag="HC643_RS11195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011190"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_038072550.1"
                     /transl_table=11
                     /translation="MSDTLTKLTYQTFQQGKSYFGLTHKILSIRLKELLFPKKEATSQG
                     IPREAILTLQQRQNNLQEVDWEDAERGVYPKSLLFDNPWEDFFRYYPLIWVDLPQIWDR
                     ANQRKYQDFAADVDIKGYPSYYIQNFHHQTNGYLSDLSANLYDLQVEILFQGSADAMRR
                     RILAPLKKGLEVFRDLAPRQTRVLDVACGTGRTLKMIRAALHQASLFGTDLSPAYLRKA
                     NELLSEIPGELPQLLQANAEELPYVDNYFHAVTSVFLFHELPATARQRVIEECFRVTKP
                     GGVFIICDSIQLSDSPEMEPFMEYFHETFHEPYYKHYITDDLVERLEKAGFVNIEMQVH
                     FMSKYLIARKAA"
     misc_feature    complement(17990..18006)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 23.25"
                     /tool="antismash"
     gene            18065..18265
                     /locus_tag="HC643_RS11200"
                     /old_locus_tag="DA73_0400011195"
     CDS             18065..18265
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453750.1"
                     /locus_tag="HC643_RS11200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011195"
                     /product="hypothetical protein"
                     /protein_id="WP_038072606.1"
                     /transl_table=11
                     /translation="MSTQEKARALMMRHYQLIKNRQQSMLGRAGEELGLPGEVSHYWNP
                     TQGKIDPSARMTYDRSNAAMS"
     gene            complement(18469..19884)
                     /gene="glnA"
                     /locus_tag="HC643_RS11205"
                     /old_locus_tag="DA73_0400011200"
     CDS             complement(18469..19884)
                     /EC_number="6.3.1.2"
                     /GO_function="GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="glnA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017747008.1"
                     /locus_tag="HC643_RS11205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DA73_0400011200"
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="WP_038072552.1"
                     /transl_table=11
                     /translation="MTTPQEVLKMIQDNNIQMIDLKFIDMPGIWQHLTLYQNQIDETSF
                     TDGVPFDGSSIRGWKAINESDMAMVLDANTAWIDPFMQEPTLSVICSIKEPRTGEWYSR
                     CPRVIAQKAVDYLVKTGLGDTAFFGPEAEFFIFDDVRFDQNQHSGYYYVDSVEGRWNSG
                     KDEGPNLGYKPRYKEGYFPVAPTDTSQDMRTEMLLTMAKCGVPIEKHHHEVATGGQCEL
                     GFRFGKLIEAADWLLTYKYVIKNVARKYGKTVTFMPKPVFQDNGSGMHTHQSIWKDGQP
                     LFAGDQYAGLSEMALHYIGGILKHAPALLAITNPTTNSYKRLVPGYEAPVNLAYSQGNR
                     SASIRIPLSGSNPKAKRLEFRCPDATSNPYLAFAAMLCAGIDGIKNKIDPGQPLDKNIY
                     ELSPEELAQVPSTPGSLELALEALENDHAFLTEPGVFTEDFIQTWISYKLDNEVNPMRL
                     RPHPYEFSLYYDV"
     misc_feature    complement(20233..20249)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 23.74"
                     /tool="antismash"
     misc_feature    complement(20234..20248)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 21.11"
                     /tool="antismash"
     misc_feature    20286..20304
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.55"
                     /tool="antismash"
ORIGIN
        1 ttattgcgga ttgcgtcgct actcttctgg aaatctgttg aatatgcgtg gttagagatt
       61 gccaaaaact aaactatcta ttttgtggga tgggtggagt atccatccct tgttttgaat
      121 cgtcgagcta ccttcgatat cttatttagc gttgctcata ctacttgtcg caattgataa
      181 tttactcggc aaaatcagtt caaaacatta cgaaatttac tatttttctt tgtatattaa
      241 tatagttggc cgagatagaa tattatttta tatctataaa atattggttt aattgtttgt
      301 gccaatacta atgttaaaat tgagaattta aaaagtatga ttgctaaaaa atgtagacat
      361 aagatcgcta aaacttaagc attaactgca tattctattt tatctaagta ggtaggcaca
      421 attaaaccga actatataaa cttatgtaaa gtgtcacaaa tgctttaaag attaagcttt
      481 taaatgattt acatttctta atgtagttgt gttttttagc gcccacctac ttaattagtc
      541 aatatagaaa tctcaattgt cagaaaaaat cctgaaagaa tacgttatgt ttatttcgct
      601 tcttgactgg atattgttaa tcaagcgagt ctcctccgga gactctaagc ggacgtttat
      661 atatagataa cgttatgaaa ttcaaaatat cctacaacac atatggattt attttaggca
      721 ttggactcat ttttaactgt gcattagcaa taagtatata tcagaaatct tctgacatag
      781 aaacttacat aaatagcttt tgtaagaatc agttgaatgg aaaaagtttt acgagaaccg
      841 agctattgtt tggtttatca aaatcagacg gctcaagggt gaacgaaaaa gaatttcagt
      901 ttttcataga caatgaagtt acacctcttt ttcctgatgg gctaaccttg ttaactggta
      961 agggtcaatt caagaattcg cgtaaaatag ttgtcaaaga agggtcgaag cttttaatat
     1021 tactttatcc ttttaacagt gacagtaacc gaaaaattca acaaattcga caggcatatg
     1081 taaaaaattt tcaacaagag tctgtgttgc gtgttgatga actatcatgc gtatctttct
     1141 aataaatcaa tcgatcgcaa gattttgata ttgttacctt cgttcacgtt gaaaatgatg
     1201 ggtatggcaa gcaaaagtta gcagagttta ttgctgaagc ttgtactaaa tttgtggagt
     1261 taacagcagc attataaaaa ttctgccatc agtaggttta tgcccatagg ttgagtgtac
     1321 taaagtggca attgttttta gatatacatc taccttggag ggtgcatgcc ccagtacttt
     1381 ggaaaattgc cgacaactga ccaaccttgg actactattg gtagggtaca atctgtcaac
     1441 aagagcgatg ctgcaaagca gccgcttcgc gaacgcgtta ttcacctaga gtgtggtgaa
     1501 gcccgtgtta gtattagcgt actcgcatcc aacttgctac gagtgcggat gtccccaact
     1561 ggtgaatttc aaccccgtcg tccttgggcg gtaacactgg acgacacaga atggacaaat
     1621 gtacctttcg aggtaaacgt aacagatagt gttgtagaaa ttgaaaccga gcaaatacgc
     1681 gtgagcgtac agagagaaaa atgccgtata acctgctttg ataaagaagg acgtcccttt
     1741 gctcaagata cagagatggc aatgggttgg cgattgggag cagtcgccgc atggaaacac
     1801 atagaagcag aagaacactt ttacggattt ggcgaacgca ctggattgct ggataaactg
     1861 agcgaagtca aaaccaattg gacggttgat gctttagact acagtaccca aactgatgaa
     1921 atgtatcagg caataccgtt ttttatagcc ttgcgtcccc aacttgcata cggtattttc
     1981 ttcaacacca ctttttggag tcagtttgat attggtgcag aagcaccggg taccttaaaa
     2041 atggaaactc gtgggagcga gttagattac tacattattt acggtcctga acctgcagac
     2101 attcttcgca cttacaccca gttaacaggt agaatggcac taccaccaaa atggtcactt
     2161 gggtatcacc aatgtcgttg gagttacgag tcagaaacta tagtacggga actagcgcaa
     2221 gaatttcgat cgcgtcaaat tccctgtgat gtcatccatc ttgacattga ttacatgcgg
     2281 ggttaccgcg tgtttacctg gagtcccaac cgcttttccg atcctgcaaa actgattggt
     2341 gacttgggac aaaacggttt taaaaccgtg accattatag atccaggagt gaaatacgag
     2401 ccagaagcca attaccacgt gtttgaccaa gggataaaac acgactattt tgtcagaaaa
     2461 gtggatggac agttgttcca cggctatgtt tggcctgaaa aatccgtgtt tcctgacttt
     2521 ttgcgttctg atgtgcgtca ttggtgggga gatttgcaaa aaagcctgac tgatgcaggc
     2581 gttgcgggaa tatggaatga tatgaacgaa ccagccattg acgatcgccc ttttggggat
     2641 ggtggaaata aaatttggtt tcctctagat gcacctcaag gaggggataa ggaagacaag
     2701 ggagacaagg gggaaggttc taccgtgtct cccttgtcta ctttgctctc acatagagct
     2761 actcacgctg aagtacataa tttgtatggt ttgtcaatgg cgagagcgag tgcagaaggt
     2821 ttgcaacggt tgcgtcccaa tgagcgttct tttgtgctga ctcgttctgg ttttgcagga
     2881 gtgcagcgat ggtcatcggt gtggatgggt gataatcagt cgttgtggga tcatttagaa
     2941 atgtccctac caatgctgtg taatatgggg ctgtctggag tagcttttgt aggatgcgat
     3001 attggtggat ttgcaggtaa tgccacagca gaaatgtttg ctcggtggat gcaggtagga
     3061 atgctctacc ccttcatgcg aggtcactca gctatgagta cagcacgcca cgagccgtgg
     3121 gtgtttggag aacgtaccga gaaaatttgt agagaataca tcaatctacg ttatcaactg
     3181 ctaccctacc tttataccct cttttgggaa gcagcaacca cagggactcc tattttaaga
     3241 ccgttactct accattaccc caacgatccc aaaacttata ccctttacga ccaggtttta
     3301 ctcggttctc atttgatggc tgcacctgtt taccgtcctg gagtagagca ccgttccgta
     3361 tatttgcctg aaggaacttg gtatgattgg tggacgggag agagctatca cggtccggtt
     3421 cacatcctcg ctcatgcacc attagaaaca atgcctcttt atgtccgcgc cggggcaatt
     3481 attcccatgc agcccgtgat gcagtatgtc gatgagcgac cgctaaacca attgcgattg
     3541 cgtatttggc atggtgctag tgagtttacc ctttatgaag atgacggaca tacatttgag
     3601 tttcaaaaag gagcttttgc aacgacaaat taccgtgttt ttgctaatgc agaacacaca
     3661 attttagaaa ttgaagaacg acaaggagaa tggataccac catcccgtga agtcattgtt
     3721 gaacttgttg gagtgggtaa acaacaattt caagatgatg gaaaaccgcg tactttgaaa
     3781 ttttaatagt tagtaagggc gcaaggcttt gcgcccctat actgataatt tctctgccag
     3841 ttgcataagt tctgtttgac aaaaaaagaa tttatgtaaa aataacatat gtgataataa
     3901 taatacattg tattagaatc gatctcaaat ttatttctaa gctgcgtcta ctttgaaatc
     3961 catagttttt accttagaga aaaactcaag ttttcgatct gggttgggtt tttctgtttg
     4021 ctatagcctc aaatcttgct ctacctgaag caaacagatt gagagtttca tgcatctatt
     4081 tttaaaaaac atttaaaaac acctaggaga tgcaacgatg ttaagtagca cctatgacca
     4141 aggtttagtg gcgctatcga tagtgctggc agtactttca tcctatacag ccttagacct
     4201 agccggaaga gtgagtgcag tggagaaaag ggtaaggctg gcttggttga ttggtggtgc
     4261 aattacgatg ggtattggga tctggtcaat gcactttgtt gccatgctag cctttagttt
     4321 accaatacct atcgtctatg atgtgggaat tgttgtattc tcaataacgc ccgcgatcgc
     4381 gacttcagca ggtgcgcttt ttcttgcaag tcgtcggttt atgagtatga agcgattgct
     4441 cactggcggt gtattaatgg gtttaggaat tgcatctatg cattatattg gtatggcagc
     4501 gatgcggatg gatgcctcta ctcactacga tatcctattg tttgtgctgt cagttgcgat
     4561 cgcgatcggc gcgtcaatag cagcgttgta tattgccttt caattccgca tacaaacgag
     4621 taaaacagga agatcgtctc ggattttcag tgcgatcgtt atgggggctg ctatctccgg
     4681 gatgcactat acaggaatgg cggcagtttc tttcacacca acaaaagttg tgagttcagt
     4741 cttagcaaac cgggttgtgc aatcttctct cacttggctt gcgatcggca ttggcatggc
     4801 tacactcgtt gtcttaagtg ttacactgct gacatcattt gcggatcgac gcataacatc
     4861 tcaaacaact cttctgaaac aacaagaagt tgaagcgcaa cgattgcaac aatttacaga
     4921 aatgactcta cgcattcggc gttctctgaa gttggaagat gttctcaata ctgctgtgag
     4981 tgaagtgcag caagtattag gtgccgatcg cgctattatc cgtcggttga atcgtgattg
     5041 gagcggtacc attattgctg aatccgttgc tcaaggttgg atttctactt tgggtcatac
     5101 aatggacgat tctttttggg aacgttatct cgaatcttat aataatggtc gagtttgggt
     5161 tattgataat atttaccaag tgggttttgc tgaggagcat ctggcgattt taaagcactt
     5221 tcagattaaa gcttatatag ctgcacctat cctacaaaac gatcgacttc agggtttact
     5281 ttttgctcat cagtgttcta atgctcggat ttggcaaaag tgggaaattg aattgtttcg
     5341 acaactcgca gttcaaattg gcattgctct agaacaagca agtctcctcc atgaattaca
     5401 acaagcgcag gaagtgctgc ggttacgcga gcgtgccatt gctgctgcta gcaatggtat
     5461 tctcattacc gctccactac aggaagataa ccccatcgtc ttttgcaatg cagcatttga
     5521 aaccatgacg ggttactcac tagaagaagt attggggcgt aactgtcgct ttttacaggg
     5581 agaaggcaca aaccctgcta ctgtaagaga aatcaacaat gccgtgaagc aagagcggga
     5641 ttgccaagtt gttattaaga attatcgcaa agatggtacc cctttctgga accaattggc
     5701 catttcacca gtgcgaaatg cctccgggca agttataaat tttattgggg tacaagcgga
     5761 catcacagaa caaatgcagg tacaagaaca attgcggctt agtaaagaaa atttacagaa
     5821 tcaagtcgta gaattgttaa gtgatgttga agaagcgtct agaggtgacc tgaccgtgca
     5881 tgcccagatg agtaagggcg agattggtat tgtagccaac tttttcaatg ctattattga
     5941 aagcctgcgc cacattgtga ttcaagtaaa acaagccgtt caacaggtga atgtacttgt
     6001 aggggaaaac tcacaagcga cgcgtctgtt ggcagatgat gcactcaaac aagcggatga
     6061 gattactcgt accctaaaat ctttagatga aatggtactt tcaattcaag ctgtagcaga
     6121 tagcgcccat aaagcagcag ttatggcacg tactgcatca acaactgcag aagttggtag
     6181 agattcaatg gagcgcacag ttgatagcat cacgaaccta catttgacta tggcagaagc
     6241 tgctaaaaag gtgaaacagc ttggtgaatc atctcaaaag atttccaaag ttgtgtcttt
     6301 aattaaccaa attgccttgc aaaccaacgt aatatcaatc aatgctagta ttgaggcagc
     6361 aaaagcaggt gaggaaggtc aggcttttgc agttgtggca gaagaagttg gggatttagc
     6421 tgctcattca gctcaagcga cggaagaaat tcaacagatt gtacgaaata tacagattga
     6481 aactagtgaa gttgtgaaag ctgtggaaca aggcatgaca caggttgtgg aaggcacaga
     6541 ttcagtaaaa gataccaagc aacagcttga gaaaatttta gaagtgtctc gtgaaattga
     6601 cttgttagta cagtctattt cccatgcaac agtatctcaa gcgcaaactt cgcaagaggt
     6661 agcgtctttg atgaaacaga ttgctcaaga ttcacttcat acgagcgatt tctctcgctt
     6721 gatgtctagt tccttggaaa aaactgtaga cgtagcacaa cagttacaag cttcagtcac
     6781 tgtgttcaag accgatcctg aggaaaggct gagtgttgaa cctttggaag cagcagatat
     6841 tgctcagtct tctggtcggg attctgttgt gttaaagatg acacaataat caaggcgaat
     6901 gaaaaattaa ggtaatagaa aattgaggcg aatagaattc gcggtcatgt agagacgtag
     6961 cacgttacgt ctctacataa cgactaacct tcgtacacaa tcaaatgagt tggtttgtga
     7021 gtgcgaataa agtttgtcat aatgtcccgt tctttaacag ttggtgttgc aaaacaacca
     7081 gcagtaccag tgttagcatt ttgagccaaa gcagagggat cgtagtgaac gcctataccc
     7141 gaacgtcccg tactaaatag aggtgtcact ggagaccaaa caccaccaaa ttctggttta
     7201 tttcctggtg gtgtacccac cactccgggg ttgtcaaact tgtaaattcc aaaaggtaca
     7261 ggtgtttcag aaccagcaac atcggaggga gtttgcttgc caactcgacc ggaaacagct
     7321 tgtactgtat taataacgtt accattgcta tctataagcc gagtttcata aactcttaaa
     7381 ccaccaccga tggtttttgt cgttcttttt gcaacaactc gtgctgtttt gatacttgta
     7441 agaaactcat tagcgaccca gcgatttgtt ccaatttgta cccacttcct gtttcctgct
     7501 tgcacgggtt cgccagtcac ttgaacttca gtaccattgc tgagagaacc aactgattcc
     7561 ccattgggtt tagaacgcac tgtgagagga tcgccttctt ttgttgcaac gtaaaaaata
     7621 ctagccatta atatatgcct gacagatgtt aaaagtaatg aaaacttttc ctgctgggaa
     7681 ttgtttttgc tgacatcaaa atacttcaat ctctgctgac aaaattacgg atagcctctt
     7741 aagagattcg gaattgcaac atccccaact tcttgatgga tacagccgat tgatgggata
     7801 agctgtgctg ccttttattt tctcatattg acctagttat aaaaaagata atcagttaag
     7861 atttatattc gtattcctct acacttaagg tatacaaaca ataagggtta aaatatttaa
     7921 ttctctaatg cagcaaccca agccccaaca aacggcagcg attcttaaag ccgaatggaa
     7981 tgacttgatt gtcgagtacg gtcgtttgca agaggtcgga gatttcgact tcgcaatgcc
     8041 caaatatgct atcagtgtag cgtttcttcc tcacgaacgt gtcacttggt cggtggatgg
     8101 gagtaagccg caaacctcgg ctttgcctgc aggaagcgtg tttctatatt cggatcggga
     8161 gtttgtatgg cacaggcgcg aaaaggagag cgaatacgta aatctgaaga tcgagccagt
     8221 gttacttaac agactggcaa atgaaagcgg gctctctact cccgttaggt tggaacacag
     8281 agtgcttttt cccgatccaa ctattctaca cgtggcgcat ttgttgaagt ccgaactagt
     8341 taatagtggt tttgcgggaa atttgtatgt tgaatcgttg cgaaatttgc tagcagtgca
     8401 tctcctcaga aattacggtg gagtcttaag tcattctact ccagaggaaa tcggtgttct
     8461 agatgtttta aaactcaagc acattaaaga ttatattgaa gacaatttag ccagtgaatt
     8521 agcgatcgca actcttgcgg cgttagtccc catgagccag tttcattttg cccgtgcttt
     8581 caaaacgctt gtaggagaac cgccacaccg ctacattctg caacgacgaa tcgaacgggc
     8641 aaaagtctta ctaaaagtga ctcggttctc gtcagcagaa attgcttacc aagtgggttt
     8701 ttccaatccg agtcacttta ccgcgcaatt tcgcaagctt acaggggtaa cgccaaagca
     8761 atttcgcgac agtgcataaa ttgaaccgag cataattttg atataaccgc gcaagaatgg
     8821 ggcgtgaatg ctttgggcag attctttaaa ttggatttaa caagaaacaa cggagaacga
     8881 acaatgaaaa caatgctttc cgctattaaa gcttctgccc tctcactagt attgttaact
     8941 agtagcgctt atgcccattc acaaaatctg tcagaaagca agaattcaac acagccgcaa
     9001 gtgcagcaac tttctgatac tgtcatcgct gctaacgcct acaaacccgg tgtaaatcga
     9061 gttacatttc aaagtgaagg cgaaacgcta gttgggaact tgtacttacc tgctagctac
     9121 aaagctggcg ataagttacc cgcagcgatc gtcgcaggtt cttggacaac agtgaaagag
     9181 caaatgccca gtatatatgc tcgaaagctt gcagaacaag gctttgcagc cttagcattt
     9241 gattttcgta cctttgggga aagcggcggt aagattcgca gttttgaatc tcccactgcc
     9301 aagatagtag atatcaaaaa tgctgttagc ttcctgcaaa ctgtcgatgc agtcgatcgc
     9361 aaccggattg ctgggttggg tgtttgcgct tcagcgggtt atatggcggt agctacagca
     9421 actgattccc gtatcaagtc atttgtcgct gttgcgcctt ggattcatga tgcagcaatt
     9481 gtaaatacca tctacggtgg tgaaaaagct gtacgggaaa aaatcaaaat tgcacaagca
     9541 gcacgcacaa aactacagca aacaggtcag gtggactatg tacctgcagc tagcgcaacg
     9601 aatggtgctg cagcaatgta cggtaatatc gactactatc ttaatcccaa acgtggtgca
     9661 attccccaat gggcaaacaa atttgcagtg atgtcttggg cggagtggtt gacctttagt
     9721 gccatgccac aagccaagca aatcaaagtc cctacattgt ttatccatag tgagaaagcc
     9781 gcaattcctg atggtgcgcg tcgattcttt gcagccatcc ctggcaataa caaaaacatg
     9841 gtttggtttc ccgaaacgca acaatttgat ttctatgacc gagaagccac tattaaccaa
     9901 gccgttgctc tcacggttaa gcaattaaga acatctctat aaaggcttga aacattggat
     9961 cgtcatttct gtaaaacttt aaaaccagga ttttgtcaag atgttaatgc aaaaagcttt
    10021 aattggcgca accttaatgc tgagtttgtt gaccatcgga cctcaaaccc aagctggtat
    10081 cacaacacac atagcaactg caaacactaa tgccgaagca atccgccaaa acaacgagca
    10141 aacaatccgc acgtacttcc agttgttatc ccaaaaacgc atggatgaat ggctaaaact
    10201 atgggcggat gatggggtac aagatatgcc ctactctcct gctggatttc ccaaacgcgt
    10261 tgaaggcaaa gccgccgtcg ccaaacatta ttcggcacta ccaactagtg ttggacgcat
    10321 ggtgttccct gacttaaaaa tttaccctac tactgacccc aatactttct ttgtagaatt
    10381 tcgtggcgaa attgaagtac tggcaacgaa aaagccttac aacaacactt acgccggact
    10441 ctttcaattc cacaatggta aggttgtact gtttcgtgaa tactacaacc ctatcgtgtt
    10501 cgtagaagct tggggtaatc ccagtggctt tactcagcag aagtaattta acagtgcaga
    10561 acaaaaagag tttcactatc actccaaaca ctttttaaat atacacgtta aggagctacg
    10621 aattatgtct ctcaacactt actacactct aggacgttct ggattgcgcg ttacccgtct
    10681 cgccctcgga gcaatgacct ttggtactga atggggttgg ggaagtgatg aagccaccgc
    10741 acgccaaatg tttaatacct acgttgatgc aggtggtaac tttatcgata cggcagattt
    10801 atataccaat gggactagcg aaacgtggtt gggtaaattt attgccgagc gcaacttgcg
    10861 cgatcgcatt gtcgttgcta ctaagtttag ctacaatgcc gaaccgggaa atccaaatgc
    10921 gggtggtaac gggcgcaaaa atattatgcg ggcggtagaa ggttcgctgc aacggttggg
    10981 aactgattat atcgacctct acattctcca tacatgggat acaatcaccc cagccgaaga
    11041 agtcatacgg acactagatg atttagttcg ttctggtaaa gtgcgccata tcgggctgag
    11101 caatacccct agttggtatg cagcacgagc gcaaactatt gccgaatggc gtaattacga
    11161 accgctttcc gctctccagc ttgaatactc cttgattgag cgtaatattg agcgggaatt
    11221 tattcccctc ggtctggaat tgggcatggg tacaatggtg tggagcccgt tggcgagcgg
    11281 tcttctcagt ggtaagtaca gacccagcga gggtggtttt acaggagaag gacgattgga
    11341 aacagttcgc ggtatctcaa atccggcgtt tcagaagttt agcgatcgca actggaaaat
    11401 tgtggcagaa cttgaaactg tggcaaaaga acttgggcgg agtatggctc aagttgcagt
    11461 caactggaca gcaaatcgcc ctggaattgc atcggtcatt attggagcta cgaagcaatc
    11521 gcaacttgag gacaatctaa aagctctctc gtttgagatt cccaccgaac ttgccaatcg
    11581 tctcgaagct atcagtcgcc ccgaaccgca atttccttac attttctttg gttcagaaat
    11641 tcaaggcatg attcatggtg gtgcaacagt tggatataaa cctgctggat accaacctaa
    11701 tgtattgatt cagtcacagg gtgctggtgt atcttaaggg ctgtataaaa cttaactcca
    11761 agtctagcaa cagactatga atcaccaacg ccggaacgtg cttttgactt ttagtgcgag
    11821 cttaatagga tttttatcta ccacagagat tgctaaggct cgcactacat ctcccactca
    11881 agtcaattct caagtcccca tgaataccat cattctacag aaattagtag atcgtgctga
    11941 aattattgat gccataaaca ttatcggtat gggtgcagat ctacgagact ggaaagcttg
    12001 tcgcggagcc ttcgccgatc gggttctgac tgactacacc tccctcaacg gtggcactcc
    12061 taatactgta gcagcagata ctttagtagc tggctgggca gactttttca gcaaaacctt
    12121 caaagccacc cagcacatga ttaccaatca tgctgtgata gtgaatggcg agcgagcaaa
    12181 ctgtacctcc cactttcggg cgcatcacgt ttatcttgag ggaaaacctg gtaaaacttg
    12241 gacgcttggg gggttctaca atcacgaact aagccatatc aacggacgtt ggaaagttac
    12301 acaaatgaag atgacttgga cttacgaaga aggtactcgt cccggttaag tttatgatgc
    12361 agtccgatat tctcgtcctc caagcctctt gggatgagct tgccatcaag tacgcgtatt
    12421 tagttaaacc acctgtttcc tcaagcaata ctttaatagt tagtattgtg acatatcaga
    12481 tagaagttac tgctgcgata ccaacgggag gctttgttaa cgcagaggaa attgaatccg
    12541 attggttgaa cgatgtccaa aaaaaaaccc tgctagatta cctgcaaatc acactcagcc
    12601 tattaagatt tgaggaaatt gttgaccaaa aaatgctttg tgccgatcgg caacccattt
    12661 taaagaggca gattactaac ccatccattc ttcatttagc acatctgcta cgagctgaaa
    12721 tggaagctcc aaaattcttc agtcaacagt ttgtttcttc aattatcaag gctctgataa
    12781 ttcattgctg gtaccaattg tgactcatct tgtcctcaat ccctgacccc taaccccccc
    12841 tttcctttta cgcagccact acctcagaat gagtttctga ggtagtggct gcatcttgca
    12901 ccatagagcg agaattattc ttacgctgca acatatttcc ggcaaatgct agcatggcgt
    12961 ttgtcttacg agtacgccac tcatctacaa gtctttctac attagcagaa ggcattcgcc
    13021 gcatcatggc ttcgtaaatg taagcagcat gaccggtttc atctttggca atgctcaaaa
    13081 ttcccaattt taagttgcga gtcactggat cgttctcggg taagacgttt gccattcggg
    13141 taaagtcttt acttgcatct aactctaaaa tataagtgct acccatgaac acatcccaat
    13201 caatgacttc cggtttgagt tgttcttgag aataaccctc gaagaaagct gcaaaaaacg
    13261 ggctgcggcg ttcttgcgat ttattttccg atttattttc ctgagtgttt tttggcaaat
    13321 ttttaaaatc tataacttct ttgttaagtt gttttaaggc atgagcaaaa atttgaccgt
    13381 gtttggtttc atctgatgcg tgttgcgcca gtttttctgc aagccatgta tcaccttctg
    13441 ctgtagcgcg atcgctaagt gcggtcagga aaggaacaga accagattct gccaattgga
    13501 accctgctag aacattgggg cgagttttag ggtcacggat ttgagcagca aagtagtagg
    13561 cgctgatacc agaaccaacc aagtgcatga agtaagtcga gaggttcatg agaaatccaa
    13621 tccgatcgat ggaaagttac atttcttata ctagtacagt tcggcgtaaa taaagcaacc
    13681 attagtgaca gagaaaaagc ttacagttaa agcttttgcg ccttctgccc tctgccatcg
    13741 tacttctgcc ttcgtgtact agtcctcatt ttttgattga ggtaataaat ttttcttgag
    13801 cggttgcaaa gtacttgcca acttttaccg atgctgttag caactcggag atggtatctc
    13861 tcattgagac tctctaagtg aggggtaaaa ccgctctaat tccctagctt gtatctgtta
    13921 acagagggag gaaatgtgtg tgagatccgt caaatttact ttaatacaag acaaactaca
    13981 ttatgtattt ataaaaaata tctaaagacg atgaaagctg gaagttttat cctccatact
    14041 ttatccttca tgctttcttg aggttcagtc atgacagatc gggtgtacgc agctgacgtc
    14101 ttagtggtgg gaggcggaac aggaggaact gcagctgctc tccaagcagc gcggcggggg
    14161 gtaaaaacta ttttggttag tgaatttcct tggttggggg gaatgctgac ttctgcggga
    14221 gtatctgcac cggatggcaa tgagttggaa gctttccaaa caggattgtg gggtgcattt
    14281 ataaaacaat tgcaacgccg tcaaccaatg gggttagaca acagttgggt cagcttcttt
    14341 agttacgacc ctcgcattgg agcagagatt ttcgcagatt gggttcaaga gttaccaaac
    14401 ttactttggc ttccaggtag aagaccttta gaggtcattc aacaaaatga ctgcgttttt
    14461 ggtgttcggt ttgcagattt tactgttact gccaaagtaa cccttgatgc cacagagttg
    14521 ggggatttgt tagatttagc tgaagtacct taccgttggg gttgggagtt gaggtctgaa
    14581 tggggagaaa ttagcgctcc agcatcattt aattatctca cagaaaaata tccagtgcaa
    14641 gcccccactt gggtagtggt tatgcaagat tttggtgaat ctatggctcc tgaaatccca
    14701 agtgcgccaa atgacaaccc ctcacaattt actggtgctt gggataattg tggtgcagaa
    14761 caatttttaa attacggacg cttgcctggg ggtctgttta tgattaattg gcccattcat
    14821 ggtaatgatt acggtcatgg agtcgggcga gtcatagcat cttcagagaa gcggcgcgag
    14881 tttctgcaag aatgcctttg gcacagccaa aattttgccc gttttatcca acatcaaatc
    14941 ggttatcgct acggtttagc ccatcatatt tttcccccat tcccccgttc ccccattccc
    15001 tctatctctc cctcatcttt tgcccttcac ccctattatc gggagagcag acgcctcgta
    15061 ggactgacga ccatacgaga acaggatatt ttaccaattg cgggaggtag cgttgcaccg
    15121 ttacatgaag atgctattgc gattggtaac tatgctaacg accatcatta tccggagata
    15181 aaatttgaat tgcttcctaa atctattcgt tggggaggac gttggacggg aactcctttt
    15241 acaattccct atcgctgtct tattcctata aaaacagatg gcttattagt ttgcgagaaa
    15301 aatatttcgg tttctcatat tgctaacggc gcgactcgat tgcaaccagt agttatgaat
    15361 attggtcaag cagctggtat ggcagctgct ttgtgtattg agttaaattt aagccctcgc
    15421 gatttgcctg taagaacttt gcaagaggct ttgttgcgcg atcgccatca tgcagctgct
    15481 atcgttccca tctttaattt accacctgat tctccggatt ggttacatat gcaactgtat
    15541 tatttagata atcctgaaac ataccctcat agcggcaatt gcccccattt ctcgccaact
    15601 gtgggcttct actcttatgg cgatgctgtt aatttaagaa acattgattg tttcatgggg
    15661 tttttccagt gccaaactca gcaggattat aaatttatta ttacttcacc aagcgattat
    15721 cagggtcaaa cttggcaaat tgtcacgttg cgatcggaca ttgacgagca gttgcaaaca
    15781 tatcttcacg gacaattcat caaaattttg ggtcgtttta acagtgcagg aaattggtta
    15841 ctagcggaac atattgagtg ctaacaagga tttttttgtg gtaaaaaaca atactcttga
    15901 ataaattatc cggatgcaca aaagaaaact cagtatcagt gaaatggata gttcaaaaat
    15961 aaacattcat tatgcgtgca gctgtttcac ttttaatcgt gggtttcctg tgtagctcct
    16021 ttgctattaa caaccaagtc cctaacactt tctcgtccaa atatgaatcg ccacaattga
    16081 ttttggcgag agcgacaaaa aaaagctcaa cctcacggtc tggacgttct ggttatagag
    16141 gtagtggacg tagagaattg attgaatata tcagtgctcc gcaatctgaa gtctaacaac
    16201 tgcaagttcg tgccagttcc tggggtggca aagaccttgt agcaagggaa taaaagtacg
    16261 gtaatccagg gtgttacgct tcctatccgt tacgtaccct ataaagcaaa aaaagtaact
    16321 tttcaattgt tggatttctc agtaatattg tggtgtttta ccagttattt cctatactag
    16381 ttaagtagtg aattcttgca ttaatgtcaa gtaaagttgc tactttgtat tgcaagtatt
    16441 gaaagatctt caaatacgca caaaactcct attgcaactt tttagttgta cggagatagt
    16501 tcattaggct aagtaagtcg gtgttcgtaa ttatcatgag tttgtagttg cgtgtctagc
    16561 cttcagcacg caagagtggc gaacgcaaga agcgcaacta cgagccaata tagagatttg
    16621 acgtttcgta acagagtttg ttttttctag cgacttactt aaaatttgca aaaaagaggg
    16681 tggctcaatc taccacccca tgtcaacaga aaagtatagg aatatagtta agcagcttta
    16741 cgagcaatca aatacttgct cataaagtga acttgcattt caatattcac aaaacctgcc
    16801 ttttccaatc gctctaccag atcgtcagta atataatgct tgtagtaagg ttcatggaaa
    16861 gtttcatgga agtattccat aaatggttcc atttctgggg aatcgctcaa ttgaattgag
    16921 tcacaaatta taaatacgcc gcctggtttt gtgaccctaa agcattcttc aataacccgt
    16981 tggcgagctg ttgcaggtaa ttcatgaaag agaaaaacag aggtgacagc atggaaatag
    17041 ttatctacgt atggcaactc ttctgcattt gcctgcaaaa gttggggtaa ttcccctggt
    17101 atttcagaca aaagctcgtt agccttacgc agatatgctg gtgacaaatc tgttccaaac
    17161 aaagatgctt gatgcaaagc cgctcgaatc atttttaaag tgcgaccggt tccgcaagct
    17221 acgtccagaa cacgggtttg acggggggct aagtctctga aaacttccaa ccctttcttt
    17281 aggggagcaa gaatgcgccg tcgcatagca tctgctgaac cttgaaagag aatttccacc
    17341 tgcaagtcat acagatttgc ggatagatcg ctcaagtagc cattggtttg gtggtggaag
    17401 ttttggatgt agtagctcgg ataaccttta atgtctacgt cagcggcaaa atcctggtat
    17461 ttcctttggt ttgccctatc ccaaatttgg ggtaaatcaa cccatattag agggtagtag
    17521 cggaaaaaat cttcccaagg gttatcaaac aacaagcttt taggatacac accccgttcg
    17581 gcatcttccc aatcaacttc ctgcaaatta ttttgccttt gttgaagcgt caatatagct
    17641 tccctaggaa taccttgaga tgttgcctcc ttcttaggaa agagcaactc tttgaggcga
    17701 atacttaaaa ttttatgggt tagaccaaaa taacttttgc cttgctgaaa agtttgataa
    17761 gtcagcttgg ttaaagtgtc agacatgaga atttccttta attaagtttt tagaatatat
    17821 ctttacatat tttaagtatt tgtaactgag tgctcataaa tctgaagaaa ggtttacagc
    17881 ttgaaaaaat aagtcttggg agagatgcaa aatcctatag atatttattc tgctacaaat
    17941 attaaatttt attaagatat tatttttcgt aactttagat acagaatagg tgttatatta
    18001 aaactaaaga aatatgtcag gtaatattga catttataaa aaaaccacta aggaggattt
    18061 cagtatgtct acacaagaaa aagcacgtgc tcttatgatg cgtcactatc agttgatcaa
    18121 gaatcgtcaa caatccatgc tgggtcgtgc gggtgaggag ctaggtcttc ctggagaagt
    18181 ttctcattat tggaatccaa ctcaaggcaa aatagacccc tcggctcgca tgacttacga
    18241 tcgcagtaat gcagcaatga gttagcacaa ggggctaggg actagggctt ggggacaagg
    18301 aagacgagga gaatgcagta taaagttctt ccttgtttcc cccttccccc caattcccaa
    18361 tccccaatca aaaaaaaacc accactagcg ggtggtttta tgttgattag ttccccgact
    18421 tttttaagga agccggggtt cgacgtactg tgcaagagtc cctaaaaatt aaacatcgta
    18481 atagagagaa aattcgtagg ggtgaggacg caagcgcatt gggttcacct cattatccaa
    18541 cttgtaggaa atccaagttt ggataaagtc ttcagtgaat acaccaggtt cggttaagaa
    18601 agcatggtca ttctccaggg cttccaaagc gagttcaaga gaacccggag ttgaaggaac
    18661 ttgagccaat tcttccggag aaagttcgta aatattctta tccaaaggtt gaccgggatc
    18721 gattttattc ttgataccat ctatcccagc acataacata gcagcaaatg ccagatatgg
    18781 gttagaagtc gcatcgggac agcggaactc caaacgcttg gctttggggt tgctaccaga
    18841 taatggaatc cgaatagaag cagaacggtt accttgagag taagccaaat ttacaggtgc
    18901 ttcataacca ggtactaagc gcttgtagga gttggtagta gggttagtga ttgcgagcaa
    18961 tgcaggtgcg tgtttgagaa taccgccaat gtagtgcagc gccatctcac tcaagccagc
    19021 atactggtct ccagcaaaca gaggttgacc atctttccaa atagattggt gggtgtgcat
    19081 cccagatccg ttgtcttgga agacgggctt gggcatgaag gtaactgttt taccgtattt
    19141 cctggcaacg ttcttgataa cgtacttata agtcaacaac cagtcagccg cttcaatcaa
    19201 ctttccaaag cggaaaccca gttcacactg accgccagta gcaacttcat ggtggtgctt
    19261 ttcaattggt actccgcact ttgccattgt cagcagcatt tctgtccgca tatcttgcga
    19321 agtgtccgtt ggagcaactg ggaagtaacc ctctttgtag cggggtttgt aacccaagtt
    19381 gggtccttca tctttaccgg aattccaacg accttcaacc gagtctacgt agtagtagcc
    19441 cgagtgttgg ttttggtcaa agcggacatc atcaaaaatg aaaaattcag cttcaggacc
    19501 aaagaacgct gtatcgccaa gaccggtttt caccagatag tcaacagctt tctgagcgat
    19561 aacgcgagga caacggctgt accactcacc cgtgcgaggt tccttgatgc tacagataac
    19621 actgagcgtt ggttcttgca tgaaagggtc gatccacgcc gtgttggcat cgagtaccat
    19681 tgccatgtcc gattcattga tggctttcca accccggata ctagaaccgt cgaaaggtac
    19741 gccgtccgta aaggaagttt catcgatttg gttttggtaa agcgtcaaat gctgccaaat
    19801 ccctggcata tcgatgaatt tcagatcgat catctgaatg ttgttatcct gaatcatctt
    19861 caggacttct tgtggggttg tcatggttac tccttaggct gccgatttat caatagtaaa
    19921 accagagtgt tcaatgtttg ctgaagaaag ctcgctaact gcaaatccat cagacccctg
    19981 gaatatccta gaaacaggac attagtggat tttgtatttt ttactacaaa atgccattaa
    20041 gaagcatgac atttaacttt tatttaaata atgggaattg aggattgggg attgggtaga
    20101 caaggaagat ttctattctc tctcccctgt ccgccttgtc cgccttatcc gccttatccc
    20161 tccactcccc atcccctcta ttaaaaaagt tcaccccgca gggggcaact ttgacataga
    20221 atccataaat agcgcacaga ttttgtgcag gggcttcttt taataagtgg cgcaaaaaga
    20281 taccgattgc gaacagcaat cagataaata tcaaaccata ataggattga ttgggagaaa
    20341 aaggaatgcg ggatgcagta actagcttaa ttaaaaatta tgacgttgct ggacggtatt
    20401 ttgaccggaa tgccatagac aacctcaagt cgtattttga aagtggaaca gcacgggttc
    20461 aagctgctgc gtctatcaac tcaaatgcgg cgtccattgt caagcaggct ggttctaaat
    20521 tatttgaaga acaacctgag ttgatt
//
