LOCUS       NZ_CP009287            20665 bp    DNA     circular CON 26-APR-2024
DEFINITION  Paenibacillus graminis strain DSM 15220 chromosome, complete genome.
ACCESSION   NZ_CP009287
VERSION     NZ_CP009287.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus graminis
  ORGANISM  Paenibacillus graminis
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 7166464)
  AUTHORS   den Bakker,H.C., Tsai,Y.-C., Martin,N., Korlach,J. and Wiedmann,M.
  TITLE     Comparative genomics of the Paenibacillus odorifer group
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7166464)
  AUTHORS   den Bakker,H.C., Tsai,Y.-C., Martin,N., Korlach,J. and Wiedmann,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2014) Food Science, Cornell University, Stocking
            hall, Ithaca, NY 14853, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method :: HGAP and Quiver: SMRT analysis package v. 2.0
            Coverage                          :: 136x
            Sequencing Technology             :: PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000758705.1-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 01:34:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,297
            CDSs (total)                      :: 6,174
            Genes (coding)                    :: 6,104
            CDSs (with protein)               :: 6,104
            Genes (RNA)                       :: 123
            rRNAs                             :: 10, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 28 of 70
            Pseudo Genes (incomplete)         :: 51 of 70
            Pseudo Genes (internal stop)      :: 7 of 70
            Pseudo Genes (multiple problems)  :: 16 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:17:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3035491
            Orig. end                         :: 3056156
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP009287.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20665
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="7"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20665
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20665
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10665](+)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10665
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     misc_feature    545..563
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.7"
                     /tool="antismash"
     misc_feature    complement(547..561)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: strong, score: 25.16"
                     /tool="antismash"
     misc_feature    complement(548..563)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 25.99"
                     /tool="antismash"
     gene            579..2180
                     /locus_tag="PGRAT_RS12405"
                     /old_locus_tag="PGRAT_13015"
     CDS             579..2180
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019911564.1"
                     /locus_tag="PGRAT_RS12405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13015"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025709173.1"
                     /transl_table=11
                     /translation="MQQDPNITNNKKLFYSLISIEEVTQPLTENRPAPLFQDYTLIIVS
                     EGQGWVEADERRFPVEKGAGFLFDAGAIHRIQAGEKGLCFYRIAFEIIGRGNSGHSWKE
                     RLSMSGILRPGPVSCRPYSQCALLLEAICHSRRSPEEIEGFAGHIRFQELLLLIMRANA
                     AALKTRDEHEAVQRSIRYMQEHFSEPVTVDQLAEVAGVGRARYTRIFKEMTGRIPLEYL
                     NGLRIERAQQQLLLTDDRLHDIALTVGYSNEYYFNRRFKGSVGVTPGQYRSLHQEGLRV
                     FAPFLEDYLLALGIVPVAQFSHPQWGKQEYLSLEQVPTFDISTGDWDGLSRFMPELIML
                     DDGFQRWHLEECRRIAPLLRLPFHQEDWRATLHSAAAIFGRTERVREIIRNYEHRAKRA
                     KQQLSRIVKDRSVAFLRLSSCGITLYGCEELGYTGGVLHHDLGLQPHPLVRELTRGQKR
                     VNLTAEELSHLNADHLFITFDRQEGEGRELLDTSLWKRLPAVRSGCVYEVDFMAWMNYG
                     VLSHQRKIEDVLRVLA"
     gene            complement(2219..2341)
                     /locus_tag="PGRAT_RS34645"
     CDS             complement(2219..2341)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006207870.1"
                     /locus_tag="PGRAT_RS34645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_269847144.1"
                     /transl_table=11
                     /translation="MSKLKRSDKAVVSFLLGLASLLIIAFFIIRYIVQNVSFAP"
     gene            2778..2891
                     /locus_tag="PGRAT_RS34025"
     CDS             2778..2891
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013372853.1"
                     /locus_tag="PGRAT_RS34025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YjcZ family sporulation protein"
                     /protein_id="WP_208619445.1"
                     /transl_table=11
                     /translation="MGENVGGYGGGYGAFTSTGAILVLFILLVIISKTILL"
     gene            complement(2989..4695)
                     /locus_tag="PGRAT_RS12415"
                     /old_locus_tag="PGRAT_13030"
     CDS             complement(2989..4695)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593850.1"
                     /locus_tag="PGRAT_RS12415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13030"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_025706780.1"
                     /transl_table=11
                     /translation="MKQAVREHKVLLTITLLCSILTSAAGVLIALILQKVIDAALQGDK
                     QLFREILLLSVVYLLLLGLFGLIYSLCSKALIRNVTITLRGRVFRGVFRQKAETFSATN
                     TADYLSALTNDIKLLEDNYIQPMLQVLQNVVVFAVSLGILLYLSPLVTVILIGCMVLMF
                     AIPSLFGKALQHKQSEVSSQMSAFTASLKDLLSGYEVITSYAMGRYAEQTFKQQNHRAA
                     GVRFAADRLFAFNESVSHTLAILTQFAVVFIAAYLIITGNLSAGSLVALVQLSGGFVGP
                     VLAIMENLPKIQGIKPVQERIDLFSEEKHEPDAEGLTPVFHSTLEARHLQFSYKQGNPV
                     IQDISLSLEKGKKYALVGPSGCGKSTLVKLLTGYYDRFDGSIALDGTDLKRLDREKLQQ
                     MVSTIHQNVYMFDSDIRHNICLHEEFSEQMLDTALHTSGIHKFLQNTPDGLLSPVGENG
                     LQLSGGQRQRIAVARALIRNKPILVLDEGTSAIDMQTAYEIESRLLKLNELTLITITHN
                     MNEDLLGLYDQIIYMENGKIAEMGSLEELLKHGGRFRGFYTLEKDLAGATG"
     gene            complement(4844..5887)
                     /locus_tag="PGRAT_RS12420"
                     /old_locus_tag="PGRAT_13035"
     CDS             complement(4844..5887)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /locus_tag="PGRAT_RS12420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13035"
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_025706781.1"
                     /transl_table=11
                     /translation="MNLDIREQLDPLFETMGLLYVSSNREQHKEQTIMELDKLGIDGEA
                     FYNKNLKIIDKYIQAFQKHQITEDSDRLFFTDQTYFSVLLALLCENANWLTELEKVSED
                     RIREALLQVLISEHSEQHSSAIEDTHLNVRSLDEIISFLEEYPFEEGVKWKLMSLLQQP
                     RKHMLALVSAVSRNLPAFEQACKEVEKPLGKLIPKLVQSIHKNGDEGFLKLVEFFSQSS
                     TLHPALIMPLGQAVYVSQCYCGLYVDLLPISGNHIADPKEYLLMRLKALADNSKLQILS
                     SLKASPKYNLEIAEQLGLSAATVSHHMNVLLASGMVGIDKKNSKVHYHLETDNIRLLID
                     ELERYLL"
     gene            complement(5986..6504)
                     /locus_tag="PGRAT_RS12425"
                     /old_locus_tag="PGRAT_13040"
     CDS             complement(5986..6504)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179175.1"
                     /locus_tag="PGRAT_RS12425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13040"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_025706782.1"
                     /transl_table=11
                     /translation="MTIHITKCGLKDSFQLQEVSYETFKETFKDQNSPENMKAYLEKAF
                     NLEQLENELSNIFTHFFLIYSNNEAAGYLKVNTNEAQSEEMGDEALEIERIYIKHKFQK
                     HGLGKVLLNKAIEMAMEHRKKKIWLGVWEKNENAIAFYKKMGFVQTGVHSFYMGDEEQM
                     DIIMTKTLI"
     gene            complement(6529..6981)
                     /locus_tag="PGRAT_RS12430"
                     /old_locus_tag="PGRAT_13045"
     CDS             complement(6529..6981)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658994.1"
                     /locus_tag="PGRAT_RS12430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13045"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_025706783.1"
                     /transl_table=11
                     /translation="MKEILREIGMIARALDSLSNIEFKEYDLTKGQYLYLVRICENPGI
                     IQEKLAEMIKVDRSTATRAIKKLEINGFIEKKEDKHNKKNKKLFPTEKGQHVYPFIKRE
                     NDHSNRVALEGFSERELETMFTLLQRARKNIEKDWEFVKKGNKRIY"
     misc_feature    7237..7252
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.87"
                     /tool="antismash"
     misc_feature    complement(7238..7251)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 23.04"
                     /tool="antismash"
     misc_feature    7261..7274
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.39"
                     /tool="antismash"
     gene            7294..7491
                     /locus_tag="PGRAT_RS12435"
                     /old_locus_tag="PGRAT_13050"
     CDS             7294..7491
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017687387.1"
                     /locus_tag="PGRAT_RS12435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13050"
                     /product="hypothetical protein"
                     /protein_id="WP_025706784.1"
                     /transl_table=11
                     /translation="MQIRDGQMIEIVYMDKLGNISQRKIEVVGIRDGRLRAHCLTAGAP
                     RVFIIANILAWRRVGEKRYA"
     gene            7484..8746
                     /locus_tag="PGRAT_RS12440"
                     /old_locus_tag="PGRAT_13055"
     CDS             7484..8746
                     /EC_number="2.7.7.7"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017249438.1"
                     /locus_tag="PGRAT_RS12440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13055"
                     /product="DNA polymerase IV"
                     /protein_id="WP_025706785.1"
                     /transl_table=11
                     /translation="MPKDRVILLSDCQAFYASVEKAAHPEYRDKPIAVGDPSRMNGIVL
                     AACPIAKAQGVTTASRVGEALAKCPELIVIRPRMGTYIKVSLLISEIYRTYTDLVEPYS
                     IDEQFLDVTGSLAYFGGSLKDMIQKIQHHVLLSTGVWTRIGIGSTKVMAKMATDNYAKK
                     MKEGIYELTFNQLESTLWRLPVQDMFMVASRMTKNFWRMGISTIGDIARMELPEFKQRM
                     RTTMGKQSDIQAEYYWQTARGLDPSPVVTGIRHQIKSVGHGKALRWNLYTRLTDIEVVL
                     LELVIEVCQRARRYRYMGAVVSISVLETDGESSCSYERQMTLPEPSSLTHEVAAAALTL
                     FMDNWSGLPVGRLAITLSRLSDDGVIQLTLFDDRVRSYNKERAIDGIKARYGNQALIRA
                     SSLLESGVARERAEQIGGHYR"
     gene            8743..9216
                     /locus_tag="PGRAT_RS12445"
                     /old_locus_tag="PGRAT_13060"
     CDS             8743..9216
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010498244.1"
                     /locus_tag="PGRAT_RS12445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13060"
                     /product="hypothetical protein"
                     /protein_id="WP_025706786.1"
                     /transl_table=11
                     /translation="MSKLDGNERWKTKMIMTEHVEQFEGQQRDAGGKMITIEERTMVRD
                     LILLPYMDTMVSKSLKELEHSGNILKRAYSMAGQAIQRRIMQDTYQLQKELKQRNIRVL
                     ADEQEEFLVYYKIFCRGYQERFGLTRDVMRTEISLRLTQYTADLGSVLRDHLK"
     gene            9469..10008
                     /locus_tag="PGRAT_RS12450"
                     /old_locus_tag="PGRAT_13065"
     CDS             9469..10008
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PGRAT_RS12450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PGRAT_13065"
                     /product="hypothetical protein"
                     /protein_id="WP_155990453.1"
                     /transl_table=11
                     /translation="MNYIWQALFMVLLINLQSYIMRNEFSLSYLVPLITILLFAIFFKA
                     VVKIPLIWSLIVTVLGYVAYAFLQTGLAKLLFGSIAAAQGEASNGYLLQFASGLITILL
                     SVLLYKIGWGFKFDFERLRFKFEDMLMVLLIIIFLVSVSVIFYYNDLFVNILFFASVMM
                     FLLYYAIRKEAQEDID"
     gene            10001..10531
                     /locus_tag="PGRAT_RS12455"
                     /old_locus_tag="PGRAT_13070"
     CDS             10001..10531
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281451.1"
                     /locus_tag="PGRAT_RS12455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13070"
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_025706788.1"
                     /sec_met_domain="AgrB (E-value: 6.1e-19, bitscore: 66.6,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDVISTRLAAGIKNVVPDHPASHAVLKFAIAVVLNVLSIISLTL
                     LISLFTGRLTEAGLILISFALLRQVSGGVHLKSGTQCVVFTTALFTLLSFVELGNVYVQ
                     ALNAVSLALVLWLAPIGIERQTRIPKRHWPKLKIAAGILIIFNMLFCSAVVAVSFFAQA
                     CTLFLAWRGVKSI"
     gene            10528..10665
                     /locus_tag="PGRAT_RS32330"
     CDS             10528..10665
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: TIGR04223"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /locus_tag="PGRAT_RS32330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="WP_081758884.1"
                     /sec_met_domain="TIGR04223 (E-value: 3e-07, bitscore: 28.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKDIQRKAVYGLASVLSGMAALVVLVSTSIVYINQPEVPEELLK
                     "
     gene            11128..11445
                     /locus_tag="PGRAT_RS12465"
                     /old_locus_tag="PGRAT_13085"
     CDS             11128..11445
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PGRAT_RS12465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PGRAT_13085"
                     /product="hypothetical protein"
                     /protein_id="WP_042266678.1"
                     /transl_table=11
                     /translation="MKVGICSSGCIKVVIDLKVHENGSLIAMTNFGDYFIIPSYNNLDE
                     ATEYSPFPDFLKRISEHDLANLYQISDVDYENKRIIFIGGSQKSVGEEYWTDFINSYEQ
                     Q"
     gene            complement(11461..11886)
                     /locus_tag="PGRAT_RS12470"
                     /old_locus_tag="PGRAT_13090"
     CDS             complement(11461..11886)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PGRAT_RS12470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PGRAT_13090"
                     /product="hypothetical protein"
                     /protein_id="WP_025706791.1"
                     /transl_table=11
                     /translation="MATIISYGSQAKIPLDPLTITTTVVGTPGAGAGLATAQVNISPAN
                     PASFSSRVELNGSFSLTALANSQFFVIIRRAGVDIYRSFYRFTTTTDIQINVLWVDGPY
                     LGVHNYELVIQNDSNLPINLFGPIAFTATAIGGEGVI"
     gene            12192..12485
                     /locus_tag="PGRAT_RS12475"
                     /old_locus_tag="PGRAT_13095"
     CDS             12192..12485
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019910556.1"
                     /locus_tag="PGRAT_RS12475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13095"
                     /product="YciI family protein"
                     /protein_id="WP_025706792.1"
                     /transl_table=11
                     /translation="MFIVNLSYIKSISEVENHLEEHVRFLEKYYNSNKFIFSGRKVPRT
                     GGVILINATSKEELDFILKEDPFYQNKIAQYEVIEVEITKYDDRFATFLMKE"
     misc_feature    12236..12250
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.67"
                     /tool="antismash"
     gene            complement(12900..13355)
                     /locus_tag="PGRAT_RS12485"
                     /old_locus_tag="PGRAT_13105"
     CDS             complement(12900..13355)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017688321.1"
                     /locus_tag="PGRAT_RS12485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13105"
                     /product="ferritin-like domain-containing protein"
                     /protein_id="WP_025706793.1"
                     /transl_table=11
                     /translation="MYMVYPYWFRSQFVPIWATSTPDALELMRSSVQGERNDELFYDQL
                     IQLAPNPEQAEVITSIRNDERGHNQMFRQMYRELTGYEVTGVSNEVPEQVTSYTAGLQK
                     AFQGELSAVEKYRKIWFGLPYGVYKDTLYGIILDEQKHAAKYNNLLL"
     misc_feature    13534..13543
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 17.14"
                     /tool="antismash"
     misc_feature    complement(13621..13634)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 23.01"
                     /tool="antismash"
     misc_feature    complement(13934..13947)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 24.68"
                     /tool="antismash"
     gene            13976..14941
                     /locus_tag="PGRAT_RS12490"
                     /old_locus_tag="PGRAT_13110"
     CDS             13976..14941
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841534.1"
                     /locus_tag="PGRAT_RS12490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13110"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_025706794.1"
                     /transl_table=11
                     /translation="MYEWQKQIQIIVDEIDKYIKNYNDEALTLRFLSRRLGYSDFYTTR
                     KFKEISGMQFRDYLRHRKLAFALKEVRDSEKSLLDIACDYGFSSHEAFTRALKGTYGVT
                     PSEYRKKPKPVVLRTKINPFDRYFLGLGEIGMLKSTDHVKIYFVTIPAHKFLHIKNYES
                     NGYWDFWQKQSLIPGQDCETICGLLDSIKGKLDDDGGSESNGGSGQIMAYINDPDGRLC
                     DWGIPRTECHGVRLPFDYKGEVPPQMLMVDVPEAEYIVFEHGPFDYEQENRSVEEKIEK
                     AMATFDFAGTGYCFDTSPGRIIYFYFNPDRYFKYIRPVRK"
     misc_feature    14948..14963
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.0"
                     /tool="antismash"
     gene            <15045..15250
                     /locus_tag="PGRAT_RS34305"
                     /pseudo=""
     CDS             <15045..15250
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019911539.1"
                     /locus_tag="PGRAT_RS34305"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="HTHASLMLASGENVKIVSERFRHANIASHWTRTAICCQTSKGALL
                     KTSII"
     gene            15341..16225
                     /locus_tag="PGRAT_RS33870"
                     /old_locus_tag="PGRAT_13115"
     CDS             15341..16225
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010347716.1"
                     /locus_tag="PGRAT_RS33870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13115"
                     /product="discoidin domain-containing protein"
                     /protein_id="WP_042266681.1"
                     /transl_table=11
                     /translation="MKKIFLLVVSCIIFTVILAKPLVNAATTEEVNLVPVMTSNTAPSG
                     IASASSIWSTNHQAFSVFDGKPNDVGWASNVTQAGWIAYEFEASVVVNKYKLMPRGEDI
                     SFAKECPRDWTFEGWNGTDWVVLDKQANVSDWAKGVKKVFSFENGVAFKKYRLNITMNN
                     GTTAVTLGAFEMYNSTPGPTPTVTPEPTVEPTPTITPTPSPTATPSPTPEQPTGNRAIL
                     VVTMNTGLEKEFDLSMEEVNSFITWYENKQAGAGKASYAIDKHDNNKGPFTNRKDYVIF
                     DKILTFSVDEYSK"
     gene            complement(16461..17012)
                     /locus_tag="PGRAT_RS31590"
     CDS             complement(16461..17012)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PGRAT_RS31590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_025708038.1"
                     /transl_table=11
                     /translation="MIVFVILGLLAIIFIGAAVYGVKQGIEKSKKLKEEIAETGALQGI
                     YVTHVEGLGIGNVECKIFRFSDRVLIDSSKQKFEIYLDQLRAAEAKSEKELIEKGKSVV
                     GRAVIGTLLVPGLGTIVGGMSGIGNKKKKGKTNHYMIFNFIDSKGELSAVTFQDVISFG
                     INDFCKNINNSPRESNVVRL"
     misc_feature    17517..17530
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 22.98"
                     /tool="antismash"
     gene            17550..17747
                     /locus_tag="PGRAT_RS12505"
                     /old_locus_tag="PGRAT_13125"
     CDS             17550..17747
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019911556.1"
                     /locus_tag="PGRAT_RS12505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13125"
                     /product="hypothetical protein"
                     /protein_id="WP_025708039.1"
                     /transl_table=11
                     /translation="MHMHIGHTVEIVYMDKAGKITQRKSEVHGIHDGRIRATCLTTGAP
                     RVFLVANIMAWQPVQERRYA"
     misc_feature    17948..17962
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: strong, score: 20.54"
                     /tool="antismash"
     gene            18073..18357
                     /locus_tag="PGRAT_RS31595"
     CDS             18073..18357
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PGRAT_RS31595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_052415712.1"
                     /transl_table=11
                     /translation="MSKLDGNERWKTKMIKTEHVEQFEGQQRDAGSKMITIEERTMVRD
                     LILLPYIDTMVRKSLKELEHSGSILKRAYLLAIEAIQRRIMQGTYLLQK"
     gene            18677..18925
                     /locus_tag="PGRAT_RS12520"
                     /old_locus_tag="PGRAT_13140"
     CDS             18677..18925
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016885857.1"
                     /locus_tag="PGRAT_RS12520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_13140"
                     /product="hypothetical protein"
                     /protein_id="WP_025708240.1"
                     /transl_table=11
                     /translation="MYAHERMAELWTISNQRSLKNSEKLEMEICQQANVIYCWDWAWFK
                     QLSLIADETNDIKMQHELCARLEELHWEVMVSDTETL"
     tRNA            complement(19339..19410)
                     /anticodon=(pos:complement(3054867..3054869),aa:Val,
                     seq:gac)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="PGRAT_RS12525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="PGRAT_13145"
                     /product="tRNA-Val"
     gene            complement(19339..19410)
                     /locus_tag="PGRAT_RS12525"
                     /old_locus_tag="PGRAT_13145"
     misc_feature    complement(19585..19598)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.09"
                     /tool="antismash"
     gene            19764..20120
                     /locus_tag="PGRAT_RS34310"
     CDS             19764..20120
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /locus_tag="PGRAT_RS34310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_238326903.1"
                     /transl_table=11
                     /translation="MGSDSSRFKVLGDYLRSRRNRLQPEQAGLSGSYNQRRTPGLRREE
                     VALLAGVSATYYTWLEQGREVTASRDIMENIGRALQLTPDENKHLMELWNPSEPEQVSS
                     INTVLNPQWQDIIR"
     gene            20121..20600
                     /locus_tag="PGRAT_RS34315"
                     /pseudo=""
     CDS             20121..20600
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /locus_tag="PGRAT_RS34315"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAYTSFISNEKSEILAWNEAAAEILIDFDSLPAPERLMLRLMFTD
                     PSLRQRMVN"
ORIGIN
        1 agaagttgcg ctcgcggaat acgaccagaa ggtggaagat ggaaaagcta agctgcagca
       61 ggcgctggga caagaaaagg tggcggtcat ccggctgaat gttgcggaca aaaccttcgc
      121 tttattcggt gtcaaaaacc gttataccgg tgtgatttat gaccaatttg gactgactcc
      181 aatcccgatg gctgccgaga tgacagatta tcaggcgatt atatcggaag aggtgattcc
      241 tgagctggaa gcggaccata ttattgtatt tcctgatcat ggaggctggg atacggcgga
      301 gaatcaggaa gcggttcaag tcctggctgg cccgctgtgg aagaacctgc ctgcggtgaa
      361 gaagggaaat atttataaaa tggaacgttc gcactggcag tccggcgcca ttacggccaa
      421 tgcgatgaag cttgatgatc tgctggaggc aatggtgaaa taaaatagaa gtaagcgcta
      481 tgaatcgtac aggaaggtct atcagcatgg ttgataggcc ttttattttg ttttacacta
      541 aggtggtgaa aatgattctc aattaggggg gcggtagaat gcagcaagac ccgaatatca
      601 ccaataacaa aaagctgttc tacagcttaa taagtataga agaagttacg caacccctca
      661 ccgaaaacag accagctcca ttgtttcagg actatacgct gattatagtt tcagaggggc
      721 agggctgggt cgaagcggac gaaagacggt ttccagtgga aaaaggtgcg ggattcctgt
      781 ttgatgccgg ggcaatccac cggattcagg caggggagaa gggactttgc ttttaccgga
      841 ttgcctttga aattattggc agggggaaca gtgggcatag ctggaaggag aggctgtcca
      901 tgagcggaat actccgcccg ggacctgtta gctgcaggcc ttactcacaa tgcgcattgc
      961 tgctcgaagc catctgccat agccgcagga gcccggaaga gatcgaagga ttcgcgggac
     1021 atatccgctt tcaagagctg ctgctgttaa ttatgcgtgc aaatgccgca gcgctgaaga
     1081 caagggatga gcatgaggcg gtacagcgtt ctatccgcta tatgcaggag cattttagtg
     1141 aaccggttac ggttgaccag cttgcggagg ttgcaggcgt gggccgtgcc cggtatacgc
     1201 ggatttttaa agagatgacg gggcggattc ccttggagta cttgaatgga ctgcggatag
     1261 aacgggcaca gcagcagctg cttctgaccg atgaccgtct gcatgatatc gcattaactg
     1321 tcggctacag caatgaatat tattttaacc ggcgttttaa gggctcggtg ggagttacgc
     1381 ccggccaata ccggagtctt caccaggaag ggctgcgtgt attcgctcct tttctggagg
     1441 attacctgct ggcgttaggg attgttccgg tggctcagtt ctcccatccc cagtggggta
     1501 aacaggagta tctcagtctg gagcaggtgc caacattcga tatttccact ggagactggg
     1561 acggcctctc ccgctttatg cccgagctga ttatgctgga tgacggcttt cagcgctggc
     1621 atctggagga gtgccgcagg attgccccgc tgctccggct cccttttcat caggaagact
     1681 ggcgggcaac cttgcactct gcagcagcca tcttcggcag gacggaacgt gtgcgggaga
     1741 tcatccgcaa ttatgagcac cgggcaaaga gggccaagca acagctcagc cgcattgtaa
     1801 aggaccggag cgtagccttc ctgcggctct cctcctgcgg aatcactcta tatggttgtg
     1861 aagagctggg gtataccgga ggcgtattgc atcatgatct ggggctgcag ccgcatccgc
     1921 tcgtccggga attgacccgg ggtcagaagc gggtgaatct taccgctgaa gagctgtcac
     1981 atcttaatgc ggatcatttg ttcattacct ttgaccggca ggagggtgaa gggcgggaac
     2041 tgctggatac ctcgctctgg aaaagactgc ccgctgtacg cagcggatgt gtatacgagg
     2101 ttgattttat ggcctggatg aattacggcg tgctgtccca tcagcgtaag attgaggatg
     2161 tactgcgcgt gctggcgtga aggcagaaga agaggcaacg tacgtgcgca acctgggctt
     2221 agggggcaaa cgatacattt tgaacaatat aacggatgat aaaaaaggcg ataatcagca
     2281 aagaagccag gccgagtaaa aaagagacga ctgctttatc ggaacgtttg agtttgctca
     2341 ttgatttcac ctctggggtt aaagctgata aaacgtttta tggtccagta aggcagcgta
     2401 agccgccctt cttgggtgga aacctccaaa acatcggaga tgggattgat ttcacatttt
     2461 ccggtatacg tttccccgct taccagatga acgcgttgac acatactggt aacaagtgcg
     2521 agctgaatat cctttttttt catttcgtca cttcccttgc ttctatctct aaaattactt
     2581 tgtattaatt atttaaaccg gattagcaat cattaacagt catgaaagtt acatctttgt
     2641 tattatttat gcatagccaa atggacctgg agaaaaagca ggtaggtcta aatatatttc
     2701 tttttcatgt atgcccaagc gtatggcgtt aatccgcata ttttaagctg tactctattt
     2761 taaggaggaa ttagattatg ggtgaaaatg ttggcggata cggcggcgga tacggtgctt
     2821 ttacttctac cggagcgatt ttggttctgt tcattttgct ggtgatcatc tctaaaacta
     2881 ttctgctgta agcttcatta ctgcaactca ctgctaacgc tcattgcaac tgcaagccaa
     2941 aaaccgccag cttgggagct ggcggttttt ggctgaggcc agacgggatt acccggtggc
     3001 tcccgccaga tctttttcca aagtataaaa acctctgaat ctgcccccat gcttaagcag
     3061 ctcctccaga cttcccatct cagcgatctt tccattctcc atataaataa tctggtcgta
     3121 taagccaagc agatcctcat tcatattatg agtgattgta atcagggtca gctcgttaag
     3181 cttcagcaat ctgctctcga tttcataagc ggtctgcata tcaatagccg aggtgccttc
     3241 atccaggacc agaatcggct tattgcggat caatgcccgg gcgacggcaa tccgctgccg
     3301 ctggcctccc gacagctgca aaccgttctc tccgacaggg gagagcaggc catccggtgt
     3361 attctgtaaa aacttatgaa ttccgctggt gtgaagggcc gtatccagca tttgctccga
     3421 aaactcctca tgcaggcata tattgtgcct gatatcggaa tcaaacatat acacattctg
     3481 atggatggtg gagaccattt gctgcagctt ctccctgtca agccgtttca aatcggtgcc
     3541 gtcaagcgca atggaaccat caaagcgatc ataatatccc gtcaacaatt tgaccaaggt
     3601 tgatttcccg cagccgctgg gacccaccag cgcgtatttt ttccctttct ccagactaag
     3661 ggaaatatct tgtattaccg gatttccttg tttgtagcta aactgcagat gcctggcctc
     3721 caatgtgctg tgaaatacag gagtaagtcc ctcggcatcc ggctcatgtt tctcttcgct
     3781 gaacaaatcg atgcgttcct gaacaggctt gatcccctgg attttgggga gattctccat
     3841 gatggccagc actggcccca caaagccgcc gcttagctga accagggcga ccaggcttcc
     3901 ggcggacaga ttgccggtaa tgatcagata ggcggcgata aaaacaaccg caaactgtgt
     3961 cagaatcgcc aaagtatggg acacgctttc gttgaaggcg aacagccggt cagcggcaaa
     4021 ccggaccccg gcggcccggt ggttttgctg tttgaaggtc tgctcggcat acctgcccat
     4081 agcataggat gtaatcacct cgtacccgga taataaatcc ttcaggcttg cggtgaacgc
     4141 ggacatttgg ctggagactt cactttgctt atgctgcagc gctttgccga atagggaagg
     4201 aatggcaaac atgagcacca tgcatccaat caagatcaca gtcaccaggg ggctgagata
     4261 gagcaatatc cccaaagaaa cagcaaatac tactacgttc tgcagaacct gcagcatagg
     4321 ctggatatag ttgtcctcca gcagcttaat atcattggtg agcgcggaca aataatcagc
     4381 ggtattggtt gctgagaagg tttcggcctt ctgccggaac acaccgcgaa acacccgccc
     4441 gcgcagtgta atcgtcacat tgcggatcag cgctttgctg cacagcgaat agatcagtcc
     4501 gaacaatccc aaaagcagca aataaacaac cgacagcagc agaatttccc ggaaaagctg
     4561 tttgtctccc tgcaaagcag catcaatcac cttttgcagg atcagggcaa taagcactcc
     4621 cgctgctgag gtgagaatac tgcataacag tgtaatggta agcagtactt tgtgctcgcg
     4681 gaccgcttgt ttcataacat tttccactcc ttaagttaga tggcggtaaa acaacaaatg
     4741 aaatatacca tataaattag attatggtca aactaattag tttaatatcg aatatttgat
     4801 tataaaaaaa actccccgca gccttgcggg aagccgttta ttttcacagc agataccgtt
     4861 ccagttcatc aatcaaaagc cttatattgt ccgtctccaa atggtagtgg actttactgt
     4921 tttttttgtc aatacccacc attccgctag ccaacagcac attcatatga tgggacactg
     4981 tagcagccga caaccctaac tgttccgcga tctccagatt gtatttggga ctggccttca
     5041 gtgaagatag tatctgcagt ttgctgttgt cggcaagcgc ctttaatctc ataagcaggt
     5101 attctttggg atcggcaata tggttaccgg agatggggag taaatccaca tacaatccgc
     5161 aataacattg tgagacgtac accgcctgac ccagaggcat gatcaaagcg ggatggagag
     5221 tactactctg gctgaaaaac tcgacgagct ttagaaaccc ctcatcgccg ttcttatgaa
     5281 tcgattgcac taacttggga atcagcttgc caagcggctt ttcgacctct ttgcaggcct
     5341 gttcaaaagc gggaagattt ctgctcaccg cacttaccag tgccaacatg tgtttacggg
     5401 gctgctgcag caagctcatc agcttccatt tcacaccttc ctcaaacgga tactcctcaa
     5461 gaaagctaat gatctcatcc aatgaacgta cattaaggtg cgtatcctca atagcagaac
     5521 tgtgctgctc actgtgttcc gagatcaata cttgaagcag tgcctccctg atccggtctt
     5581 cagacacttt ctcaagctcc gtaagccagt tggcattttc acacagcaga gccagcagca
     5641 cagagaaata cgtctgatcc gtaaaaaaca ggcggtcact gtcctcagtg atttgatgtt
     5701 tttgaaaggc ctggatgtac ttgtcgatga tcttcagatt tttattgtaa aaggcctcgc
     5761 cgtctatgcc aagtttgtcc agctccatga tcgtctgttc cttatgctgt tccctgttgc
     5821 tgctgacata aagcaagccc atggtttcaa aaagaggatc gagctgttcc cggatatcga
     5881 ggttcatggc gcctccaatt taattagtga aatttggtgt tcatctaatc tattatatgt
     5941 ctatggaagt gccttttcaa gcggatttcc aaatgcttag acccgttata taagtgtttt
     6001 ggtcattata atgtccattt gttcttcatc acccatataa aaggagtgga cccccgtttg
     6061 aacaaacccc attttcttat aaaaggcaat cgcattttca tttttttccc atacgcccag
     6121 ccagattttc tttttcctgt gttccatcgc catttctata gctttattta gcagaacttt
     6181 accaagccca tgtttttgaa atttatgctt tatataaatc ctctcaattt ccagtgcttc
     6241 atcacccatt tcttcagact gggcctcatt ggtatttacc tttaaatatc cagcggcttc
     6301 attattagaa taaatcaaaa agaagtgcgt aaaaatattg gataattcgt tttctaattg
     6361 ttctaagttg aatgcctttt ccaaataagc cttcatgttt tcaggtgagt tctgatcctt
     6421 aaacgtctcc ttaaacgttt cataactaac ttcttgaagc tggaatgaat ctttaaggcc
     6481 acactttgtt atgtgtatag tcatttaaac gggttgctcc ttttaaaatt aataaattct
     6541 tttgtttcct ttttttacaa attcccagtc tttttctata ttttttcttg ctctttggag
     6601 cagagtgaac atggtttcta attctctttc ggaaaatccc tctagtgcaa cccgattgga
     6661 atgatcattt tctcttttaa taaaaggata aacatgctgc cctttctctg taggaaagag
     6721 ctttttattt tttttgttgt gcttgtcttc tttcttttca ataaaaccgt taatttcaag
     6781 cttttttata gcacgggttg ctgttgaccg gtctactttt atcatctcag ctaacttttc
     6841 ttggatgatt cccgggtttt cacatattcg cacaaggtac aaatactgcc cttttgtaag
     6901 gtcatattct ttgaattcta tattgctcaa agaatccagt gcccttgcta tcattccaat
     6961 ttcccgaaga atttccttca ttatttaact ccttagcaga tatttttgtt gcaaatacaa
     7021 taaaagaggg cagattaaat ttaacctaac tttattgcat ttgcaacaaa aaataatttt
     7081 gccacttcac tgtttgctgt atagattgaa cttgtgattg ttctatttcg ggatgggtcg
     7141 tgactacaga gaatgtttga acttccggcc actgttgtct ggatatctct tggtttcttt
     7201 tttatcccag cgtaagaata tcttacttgt tgtttgtaga acgtatgttt ggttataata
     7261 caaacaaatg ttcctgtggt ggaggcggat cttatgcaga ttcgagacgg tcaaatgatc
     7321 gaaattgtat atatggacaa gctgggaaac atatctcagc ggaaaattga agttgtggga
     7381 atcagggacg gccggctccg ggcccattgt cttactgccg gagcaccccg cgtatttatt
     7441 attgccaata tcctcgcttg gcggcgggta ggagagaagc gctatgccta aggatcgtgt
     7501 cattctactg tccgactgcc aagcctttta tgcctcggtg gagaaagccg cccatcctga
     7561 atatagagat aaaccgatcg ccgtaggaga tccgtccaga atgaacggca ttgtactggc
     7621 agcctgcccg attgccaaag cacagggcgt aacgacggcc tcacgtgtag gtgaagcttt
     7681 agcaaaatgc ccggagctaa ttgtcatacg cccccgcatg ggaacttaca ttaaggtctc
     7741 gcttctgatc tcggaaattt atcggaccta taccgatctg gtggaaccct acagcatcga
     7801 tgagcagttt ctggatgtca caggctctct tgcttacttt ggagggtcat tgaaagacat
     7861 gattcaaaaa atccagcacc atgttttgct gtcgacaggc gtctggaccc ggataggcat
     7921 tggttccact aaagtcatgg ccaaaatggc gacggataat tacgccaaaa agatgaagga
     7981 agggatatac gagcttacct ttaaccagtt ggagtccacc ctttggagac tgccggttca
     8041 ggatatgttt atggtggcgt ctagaatgac caagaatttt tggcgcatgg gtatatccac
     8101 aatcggagac attgcacgaa tggagctgcc ggagtttaag cagcgaatgc gaaccaccat
     8161 gggcaagcag agcgatatcc aggcagaata ttattggcaa accgcgcgcg ggctcgatcc
     8221 tagtcccgta gttacaggaa tacggcatca gatcaagtcc gtggggcatg gcaaggccct
     8281 gcgctggaat ttatataccc ggctgacgga tatcgaggtt gtgctgcttg agcttgtcat
     8341 cgaggtgtgc cagcgggcac ggcggtatcg ttacatgggg gcggtggtct ctatttccgt
     8401 tttggagaca gacggagaga gctcatgctc ttatgagcgc cagatgacat tgcccgagcc
     8461 ttcatcgttg acgcatgaag tggccgcagc ggcgcttaca ttattcatgg acaactggtc
     8521 cgggctgccg gtggggcgct tagccataac gttgtcccgc ctttcggatg acggggtgat
     8581 ccagcttacg ctgttcgatg accgggtccg ctcttacaac aaggaacgcg caatcgacgg
     8641 aattaaagcc cggtatggaa atcaagccct catcagggct tcatctttac ttgaatccgg
     8701 agttgcccgg gagagagctg aacagattgg gggtcattat agatgagcaa actcgacggc
     8761 aatgaacgct ggaaaacgaa aatgattatg accgagcatg ttgagcagtt cgaggggcag
     8821 cagcgtgatg ccggaggtaa aatgataacg attgaagaga ggacgatggt ccgtgacctt
     8881 atcctgctcc cttacatgga tactatggtt agcaagagcc tcaaggagct tgagcactcc
     8941 ggcaacattc tgaaacgcgc ctattcgatg gccggccaag ccatccagcg ccggatcatg
     9001 caggatacct atcagctgca aaaagagctg aagcagcgga atatcagagt gctggcggat
     9061 gagcaggagg aatttttggt gtattacaaa atcttttgcc ggggatacca ggaacgcttt
     9121 gggctaaccc gtgatgtgat gcggacagag atcagcttaa ggctgaccca atatacagcc
     9181 gaccttgggt cggtgttaag agatcatcta aaatagaaag aaccccgcca atcgctggcg
     9241 gggtttcttc gcacgtactt gaagtaaccc cgtctgatgg ggctaattct gtagtaaata
     9301 agtactatac gttagtcctc tttccgatat ataattaatg cattgaatga aaattatgtc
     9361 aggcggtgct gtgtgttgtc atgggaacct tttggatata tggcattttc aacaattgaa
     9421 accctttcgg tgtattgtct cataatgtcg ctgttcaggt ttaaatttat gaattatatt
     9481 tggcaagcct tattcatggt gcttctcatt aatctgcaga gttatatcat gcgcaatgaa
     9541 ttttcacttt cataccttgt tccgctgatt acgattttgc tgtttgcaat cttttttaag
     9601 gctgtggtga agatcccctt aatctggtcc ttaattgtaa cggtgttggg ttatgtggca
     9661 tacgcttttt tgcaaaccgg cttggccaag ctgctattcg gatctattgc agctgctcaa
     9721 ggagaggctt cgaacggtta tttgctgcag tttgcaagcg gattaataac cattcttttg
     9781 tcagtattac tgtataaaat cggttgggga tttaagttcg attttgaacg gctccgtttt
     9841 aaattcgaag atatgctgat ggtcttatta atcataatct tcctggtctc cgtttcggtc
     9901 atcttctatt ataatgattt gttcgtgaac atattgtttt ttgcttcagt catgatgttt
     9961 ctgctctact acgctattcg aaaggaggcg caggaagaca ttgattgatg ttatttccac
    10021 aagattggca gccggaataa agaatgttgt tcctgaccac ccggcctcac acgccgtgct
    10081 taagtttgca atagccgtgg ttctaaatgt gctgtctatc atctcgttga cgttgttaat
    10141 atctctattt accggccgcc taactgaagc tgggttgata ttgatttcat tcgcactgct
    10201 gcggcaggta tccgggggag tacaccttaa atctggaacc caatgtgtag tcttcaccac
    10261 ggcattattc actcttttat cctttgtgga gctgggtaat gtgtacgttc aagcattaaa
    10321 cgctgtaagt ctggcccttg tactatggtt agcgcctata ggaattgaac ggcagactcg
    10381 tattcccaaa cgtcattggc caaaactcaa aatagctgca ggcatcctaa ttatttttaa
    10441 tatgctgttc tgttcagcag tagttgcagt aagcttcttc gcgcaggctt gtactctatt
    10501 ccttgcttgg aggggggtga aatcaatatg atcaaggata ttcagcgtaa agcggtatat
    10561 gggctggcgt ctgttttgtc aggaatggct gctttggttg tgcttgtgag caccagtatt
    10621 gtatatatca accagccgga agtgcccgaa gaactgctga aatagtttgg aggtttagca
    10681 tgagtatgat aagcgctact acagatctag agggacagtc agggcttgtt cctgtaagag
    10741 gggaagatat catatttctg ggtacagaca gctcctcaag catcatatta ttgcacactt
    10801 tggactctgt attttacaca accggtacac taaaatattg gacaagggtt ttaaatgcat
    10861 cggggtacac cttttactta gcagaccgta acaatagtat aaacatccac aacatagtgg
    10921 agatcaacag tcttctgaag gttgcttact tcgaacgcga acggaccaag gattcaaaat
    10981 attgcacaat gtccaaaagc ggcatcaaaa aagtgtgcca gcttgtggga gaactaaata
    11041 catcagtggt attcaattaa aagctatcct tcgggatagc tcaaatgggg tttcagaacc
    11101 taaccctaag ttctgaaacc cctggaaatg aaggtgggga tttgtagttc tggatgcata
    11161 aaagtagtaa ttgatttaaa ggttcatgaa aatggctcgc ttattgcgat gactaacttt
    11221 ggtgattatt ttattattcc ttcgtataat aatttggacg aggcgacgga atattcgccg
    11281 tttcccgatt tcctcaaacg tatttcagaa catgatttag caaatttata tcaaataagt
    11341 gacgttgatt atgaaaataa aaggattata tttataggag gaagtcaaaa atcagttggc
    11401 gaagaatatt ggacggattt cataaatagt tatgagcagc aatgaagcct cggagaaagc
    11461 ttaaattacg ccctctccgc caatagcagt tgctgtgaat gctatgggtc caaatagatt
    11521 tataggcaag ttgctatcat tttggattac caattcatag ttgtggactc caagataagg
    11581 accatcaacc cagagaacat ttatttgaat atcagtagtt gtagtaaaac gataaaagga
    11641 tcgataaata tcaactccag cacgccgaat tataacaaag aattgactat tagcaagtgc
    11701 agtaagacta aaactaccat ttaactcgac tctactggag aaagaggcag gattagccgg
    11761 actaatattt acttgcgctg tggctagacc cgctccagct ccaggagtcc caacaaccgt
    11821 tgtagtaata gtaagcgggt ctaatgggat ttttgcctgt gaaccataac ttataattgt
    11881 tgccatttat catcactcca tttcaatgtt atacgttatt ctattgtgaa tctgcaatta
    11941 tagattggac tgaattcctg atgattacat acatttaagt tgtactctgc ttgatcctat
    12001 aaatctagat actagcgagg gaatgtacaa aaaaactttt aacaaaagat gctgatccgc
    12061 agcatgccgg atttagtcca gtttcgcatc aggaaaattt tccttatcgt catatacata
    12121 atgttccaaa taaaacggat ggacagtcta ctgccagtct gatagcacat ttcaatttga
    12181 gggggacgta catgtttatt gtgaatttga gttatataaa gtcgataagt gaggttgaaa
    12241 atcatcttga agagcatgtc agatttcttg aaaagtatta caactcaaat aagtttatat
    12301 tctcgggaag gaaggtccct agaactggtg gtgtaatttt aattaatgcc acaagtaaag
    12361 aagaattaga ctttattttg aaggaagacc cgttttatca aaataaaatt gcccagtatg
    12421 aagttataga agttgaaatt acaaagtacg atgatcgttt cgctaccttc ctaatgaagg
    12481 agtgatcact tgagcactac ataagtcatg gagagtgatg cagacttcca cgtggcggct
    12541 ttggcacaat cgaaggtatc agtatgttgc ggccagaaga tgatccagaa gggcgtggat
    12601 tacgggggca tagttgaagg atactctcca gagagtatta agatagcggg tggtcggttc
    12661 atccgagaac gaggttccgc tttaaagtga ggacataagc tgcataaaaa aagccctgac
    12721 cagaccacgg gcttttttct atttttctgt aacaaattta tcaacgattt tttgtctgcc
    12781 ttctttgttg aagtggggat cacaatccaa agggccataa taaatcctca tacatccgta
    12841 atttactggc tgtaatgggg gcggcagtgc atgcaattac taccggtatg cagctgagtt
    12901 cacagcaata gattattata cttcgctgca tgcttctgct catcaagaat aatgccgtac
    12961 aaagtatcct tataaacgcc atatggaagg ccgaaccata tctttcggta cttttcaact
    13021 gcagacaatt caccttgaaa ggctttttgt aaaccggcag tatacgaagt aacttgttca
    13081 gggacttcat tgctaactcc tgtcacttca tatcctgtta attccctgta catttgccgg
    13141 aacatttgat tatgccctcg ttcatcattc cgaatagagg taataacttc ggcttgttca
    13201 gggttcggag caagttgaat gagttggtca taaaaaagtt catcatttcg ctcgccttgg
    13261 actgaactcc tcattaattc aagtgcatct ggagttgaag ttgcccaaat agggacaaat
    13321 tgtgatctga accaatacgg atacaccata tacatgagct taggcctcca ataattagta
    13381 agcattctat aatcaatcat atgcgcactg gcccattgca tgtgcaggat ccctcttttt
    13441 actttatgta tccttttgtt gttcatcaca ttccattgtt cgcataagca tttcctggag
    13501 ttgccgttgc tagatgaacg tagtgttcca tctgaaggcg gactaatcaa gtggttattg
    13561 ttcttaaaag gtgcggatac atacgttcat taactaatac ttgctattat gggatttcaa
    13621 ttaaaataat taaatattat gctttaaacc agccgttttt ccggtttgga gaaacggcat
    13681 ttttgtgctg atttttctta atatacctgg tgaatgtgca aatatagatt tcaaaagttg
    13741 tatggctggt actgtgtgtt gtatacgcac tttatccggt gccggaaact ggatgatgaa
    13801 catgctgcta acgacaattt ctttgataga agtattaaaa acttcatatt gtaggctaga
    13861 cgcgacgatg cagaacttaa aagaacaaat gaaggaacag caagtaaaat caagcagtga
    13921 tctctctgtt tctttataat aaatataggg tgattgaaac ggggtgatta ggcgaatgta
    13981 cgagtggcag aaacaaattc aaataatcgt tgatgaaatt gacaaatata ttaaaaatta
    14041 taatgacgaa gccttgacac tacgctttct atcccgcagg ctgggttatt ccgattttta
    14101 tacaacgaga aaattcaaag aaatatcggg tatgcaattt agggattatc tgcggcatag
    14161 aaaattagcc tttgccctaa aagaagttcg ggatagtgaa aaaagccttt tagatattgc
    14221 ttgtgattat ggtttttcat cacatgaagc ttttaccaga gcgttaaagg gaacgtatgg
    14281 tgtaactcca agcgaatacc gaaaaaagcc taagcctgtc gttcttcgta caaaaataaa
    14341 ccctttcgac cgctactttt tgggattggg agagattggt atgttgaaat ctacagacca
    14401 tgttaaaatt tattttgtaa ctatccccgc tcacaaattt ttgcacatta aaaactatga
    14461 gagtaatggg tattgggatt tttggcaaaa gcaaagtctt attccaggac aagactgcga
    14521 aaccatttgc ggcctgctcg atagtatcaa gggcaaattg gatgacgatg gcgggagcga
    14581 atctaacggc ggcagcggtc agattatggc gtacattaat gacccggacg gcagactctg
    14641 cgattggggt attccacgta cagagtgtca tggtgtacga cttccttttg attataaagg
    14701 cgaagtacca ccgcaaatgc ttatggttga tgttcccgaa gccgagtata ttgtttttga
    14761 acatgggcca ttcgattatg agcaggaaaa tcgtagtgtg gaggaaaaga ttgaaaaggc
    14821 aatggcaact tttgattttg caggcactgg ttactgcttt gatacttccc ccggtagaat
    14881 aatttacttc tattttaatc cggaccggta ttttaagtat atcagaccag tgcggaagta
    14941 gataaaatcg actgcctgtc cgctattgtt tctaacggga aaaccaaaca gaaaattcca
    15001 ctttgatgaa gtgtgcgtaa tgaatatcct ttcacagtcc ttgtcacact catgcatcat
    15061 tgatgctggc atcaggcgaa aatgtgaaga tagtgtccga gaggtttaga cacgccaata
    15121 tcgcatcaca ctggacacgt acagccatct gttgccaaac aagcaaaggg gcgctgctca
    15181 aaacttcgat aatctaatgc tagctagtga ctcctttttc aaatccacat attccctcaa
    15241 taacccttaa gtgtattgta aatttctgtc gaataacaga ctgtttgttc gcctttattt
    15301 agtgtaggct tggtacatac aatttaaaag gagagtacat atgaagaaaa tctttttgct
    15361 tgttgtatct tgtataattt ttactgtaat actggctaaa ccattagtca atgcagctac
    15421 cactgaggaa gtgaatttag tgcctgttat gacttctaac acggctcctt ctggaattgc
    15481 gagtgcgagt agtatttgga gtacgaacca ccaagccttt agtgtatttg acggaaaacc
    15541 taatgatgtt ggatgggcat ccaatgtaac ccaggctgga tggatcgctt atgagtttga
    15601 ggcctcagtc gtagttaata aatataaatt gatgccacgt ggagaagaca tcagctttgc
    15661 caaagagtgc cctcgtgatt ggacgtttga aggttggaat ggaactgatt gggtagtact
    15721 tgataaacag gcaaacgtat cggattgggc taaaggtgtc aaaaaggtgt tctcattcga
    15781 gaatggggta gcttttaaaa agtaccgatt gaatattacc atgaacaatg gtactaccgc
    15841 tgtaaccctt ggagcatttg aaatgtataa ttcaacacca ggaccaacac ctaccgtcac
    15901 accagaacca actgttgagc caacaccaac gattacgcct acaccttcac caaccgcaac
    15961 accatcgcca actccagagc aaccgaccgg taaccgtgca atcctggttg taacgatgaa
    16021 caccggcctt gaaaaagaat ttgacctgag tatggaagaa gtgaattcct ttatcacctg
    16081 gtatgagaat aagcaagccg gtgcagggaa ggcatcgtat gctattgata agcatgacaa
    16141 caacaaaggg ccgttcacca accgaaaaga ctatgtgatc tttgataaga tcctcacctt
    16201 cagtgtggat gaatattcga aatagcattt acaacttgtt ctaggcacaa taaaataaat
    16261 gtaacggatg taccgtaccc tgccagtaat ggtggggtat ttcttttcgt taaggaaaat
    16321 ttatccttat cgtcacatac attgatccag atgtgctatt gttttattac acggatcgga
    16381 agcaccgagc gtgcagtccg aaagcatcgg caaaactaaa agcccgtcaa ccttaactgg
    16441 tcggcgggct tactgttttt ttaaagtctg actacattgg attcgcgagg ggaattgttt
    16501 atgtttttgc agaagtcatt tatgccaaac gatataacgt cttgaaaagt tactgccgac
    16561 aactcccctt tagaatcgat aaaattaaat atcatataat ggttagtttt tcctttcttc
    16621 tttttgtttc ctataccgga cattcccccg acaattgtcc ctagtcctgg gacaagcagt
    16681 gtgccgatta cagcacgacc aacaacgctt tttccctttt cgattaactc tttctcactt
    16741 ttggcttcgg cggctctaag ttggtccagg tatatttcaa acttttgttt gcttgaatca
    16801 ataagtacac gatcagaaaa tctaaagatt ttgcactcga catttccgat gccaagccct
    16861 tccacgtgag ttacataaat cccttgcaat gctccggttt ccgcgatctc ttctttaagc
    16921 tttttcgatt tttcaattcc ttgttttact ccataaacgg cagcccctat aaagataatt
    16981 gcaagtaatc ctaaaatgac aaatacaatc atgattatcc ccctaaattt gataattaat
    17041 caaaatcatt ctatcaaaat tgggtaatat ttactatgta atttttaagc ttgggaatat
    17101 gctcactggt ttcggtgggc tttttgtcgt ttacataaat aaccttgtca aatatactga
    17161 ttagcaaata ctaaaattgc acttcgggag tggtttatat accaagtcaa gaggcagttt
    17221 ttaaaatgaa atcaatgttt aaccctaaag atgaattagc ccaatgggtt ctgaaaattg
    17281 ccatgtccag aaatgatttt ctatatgttc ataatcgcct tcgtgatttg caaccataat
    17341 cttggagcat attaatttgg tatttgctga tgatcttgct ttaaagctaa gtttaggtga
    17401 gatttctgag gtgtttttcc ttcagaattt gtaatgattt atcaaaagtg ataacttatc
    17461 tttaaaggag gaaaatatgg acatcttaat gtttttacct aattgtggtt ataatacaaa
    17521 caaacgttct tgtctctgga ggcgatcaaa tgcacatgca cattggtcat accgttgaaa
    17581 tagtatatat ggataaagcg ggaaagatca cgcagcgcaa gagtgaagta cacggcatcc
    17641 acgatggccg tatacgggcg acctgcctga ctaccggcgc gcctcgggta ttcctggtcg
    17701 ccaacatcat ggcctggcag ccagtgcagg agaggagata tgcttgacga taccccgcgt
    17761 aagctgctgc gtgtcatcgc acagtttcgt taccatttca ggcggatgcc gaatcttccg
    17821 gagttaaggc ggttaagcgg gcggcggcca gcggagataa acaaggggtt taaaatactg
    17881 gtcgctgaaa attacataac ctggagtccg tctcaaccta tcgagacagc cgtcattatt
    17941 gagggctgga aaaggtgttc cgtatgatac gatgccgcag ggaggcgcac agaacgcaag
    18001 tggcggcact aacatagatt gctggctgta tcattagagg ggtcacagtg accaggttgg
    18061 aggtcattat aaatgagcaa gctcgacggg aatgagcgct ggaaaacaaa aatgatcaag
    18121 actgagcatg ttgagcagtt cgaggggcag cagcgtgatg ctggtagtaa aatgattacg
    18181 attgaagaga ggacgatggt ccgtgatctc attttgcttc cttacataga taccatggtt
    18241 agaaagagcc tgaaggagtt ggaacactcc ggcagtattt tgaagcgtgc gtatctgttg
    18301 gccattgaag ccatccagcg ccggatcatg cagggtacct atctactgca gaagtagctc
    18361 aagcagcgga atatcaaggt gctggcggat gagcaggacg aatttatcct atatcacaaa
    18421 gtcttttgcc ggggatatca ggaacggttt ggattgacga gggatgttat gcgtacagag
    18481 ataagcctgc ggttaagcaa gtatattgca gagctaggga cagtattaaa agatcatctt
    18541 aaataaacga aatctcgtca gtgattgtac tggcggggtt ttgtttgctt tccgttccac
    18601 ttttattttg tcgggatggt tgcggaatcc gccatacgag ggctgccagc gtcctcgcat
    18661 catataggag gggtagttgt atgcacatga acgaatggct gaattatgga ctatcagtaa
    18721 tcaacgttct ttgaaaaatt ctgaaaaact tgaaatggag atctgccagc aagcaaacgt
    18781 aatttactgc tgggattggg catggtttaa gcagttatct ttaatagctg atgagacaaa
    18841 tgacatcaag atgcagcatg aactttgcgc tcgattagag gagctgcatt gggaagtcat
    18901 ggtgtccgat actgaaacac tataatcatc taaaagttgt tttgtaagag ctctttggtt
    18961 cattcccttt gactctcgat gataacgtaa cagacactcc cctaagatga gtgccacaat
    19021 acctccgata attaaattaa gaaggtgatg tagaaaataa taacacatga ataaattgat
    19081 gacacaatgc gggaaaaaag aaaatggtcc aaatataaaa atccggtgga cgagaaattc
    19141 ttagaatata aagttagaat cgcgttagca gtattagatg ttcctttacc agcgaaagtt
    19201 aaagtaatca ctccgaataa aaaatatcaa acaattcgtc cccaaaataa ttttcaagca
    19261 caattaagtt agttaagcga attgaagaga aaagaaaaaa acccttatat aataagggtt
    19321 tttagagatg gtgcagtata ggacggatgg ggttcgaacc catgacccct accctgtcaa
    19381 gatagtgctc tcccgctgag ctaccgtcct gcaacgaaat ttataatacc ataggatgtg
    19441 aggggaaggc aatgtttttt tctgggcagg ctcgcaaata cctgatatac aaattattct
    19501 atcgttgctg ccggaggaat agcgtaggat aagctatgat acatagaacg atcaattgct
    19561 ggagggagca agcaaggact gttttttttg cgaaaaatta cctcaatggg gcagacgctg
    19621 ggatgatctg gaatgttgct cagcggatta acagaagatt aacgcaccaa atggcagttt
    19681 gttgtggatt aaggtataag ctttatctgc tgctccggct ggggtagaat acaataaaca
    19741 tggcttggaa aagaggttgg actatgggaa gtgacagcag cagattcaaa gtgctgggcg
    19801 attatctcag atcgcggcgg aaccgtcttc agccggaaca ggctggcttg agcggttcct
    19861 acaatcagcg gcgaacgccg gggctgcggc gggaagaggt agctttgctg gccggggtca
    19921 gcgctaccta ctacacttgg ctggaacagg ggagagaggt tactgcatcc cgggacatta
    19981 tggagaatat agggagggcc ctgcagctga ctccggacga gaacaagcat ctgatggagc
    20041 tatggaatcc ctctgagccg gagcaggttt cttctattaa tacagtgctg aatccgcaat
    20101 ggcaggatat tatccggtag ttggcctata cttcttttat cagcaatgaa aaatccgaaa
    20161 ttctggcctg gaatgaggcc gccgccgaaa tactcattga ctttgactct ttgccggcac
    20221 cggagcgcct aatgcttcgc ttgatgttta ccgatccttc actgcggcag cgtatggtga
    20281 actgagagga gttcgcccgc cattccgttg ccgtttttag aacgtattat gacaagcagc
    20341 atggcgaagc tttttataag gaatttgtgg aacggctctg cgcggacagt ccggagttca
    20401 agcgcatttg ggaattgcat gatgtggagc ataaaagagt gaaccgggtg tacatccgga
    20461 aatctgctga ggcggtgcct aatgcctacg atattcactc cgttgccagt attgcagagc
    20521 atcccgatct gcatatatgc gtctacacac cggttctgaa tgctgcgcag gatataccag
    20581 agaacaccgc aggcggataa cctcatgctt ccctggtaaa gatcttacta gcataaacat
    20641 actgtggctg catcccgaag cgggg
//
