LOCUS       NZ_CP009287            23897 bp    DNA     circular CON 26-APR-2024
DEFINITION  Paenibacillus graminis strain DSM 15220 chromosome, complete genome.
ACCESSION   NZ_CP009287
VERSION     NZ_CP009287.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus graminis
  ORGANISM  Paenibacillus graminis
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 7166464)
  AUTHORS   den Bakker,H.C., Tsai,Y.-C., Martin,N., Korlach,J. and Wiedmann,M.
  TITLE     Comparative genomics of the Paenibacillus odorifer group
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7166464)
  AUTHORS   den Bakker,H.C., Tsai,Y.-C., Martin,N., Korlach,J. and Wiedmann,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2014) Food Science, Cornell University, Stocking
            hall, Ithaca, NY 14853, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method :: HGAP and Quiver: SMRT analysis package v. 2.0
            Coverage                          :: 136x
            Sequencing Technology             :: PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000758705.1-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 01:34:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,297
            CDSs (total)                      :: 6,174
            Genes (coding)                    :: 6,104
            CDSs (with protein)               :: 6,104
            Genes (RNA)                       :: 123
            rRNAs                             :: 10, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 28 of 70
            Pseudo Genes (incomplete)         :: 51 of 70
            Pseudo Genes (internal stop)      :: 7 of 70
            Pseudo Genes (multiple problems)  :: 16 of 70
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:17:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1017841
            Orig. end                         :: 1041738
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP009287.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..23897
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="2"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..23897
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..23897
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:13897](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..13897
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            177..1403
                     /locus_tag="PGRAT_RS04345"
                     /old_locus_tag="PGRAT_04540"
     CDS             177..1403
                     /EC_number="2.7.7.65"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025: diguanylate cyclase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /locus_tag="PGRAT_RS04345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04540"
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_025705304.1"
                     /transl_table=11
                     /translation="MKTTMKAAFSRTLPFLVVAAAYFLYTKINTMSNPQVEAIGLSPYA
                     IFFVGAVLSWKFNRSREFYILLILAICIASMAYLPEISGTALRSGDIYSIICLAIPLNI
                     ALFSFFKERGIISLWGSLRMALILGQCLFLFWQVKSGEREWLHLLNKEVVPVDLHTVTP
                     VPQLSVIAFVVTFVILIVRGIVYRSSSQEISFISVLLAIFYVLHQNNSPLLYSIIFAAS
                     GLVFIISIIQDSYSMAFSDELTGLPSRRALKQDMMKLGFNYAIAMLDIDFFKKFNDTYG
                     HDTGDEVLRLVASNIKEVTGGGRAFRYGGEEFTILFPGKNINDVLPHLEELREKISKQA
                     FTIRAKGRSKNKSSKRSSSARTSKQIYITVSIGVSQKNEKYKTTESVMKSADTALYRAK
                     KKGRNCVSK"
     misc_feature    complement(1573..1590)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            2024..3763
                     /locus_tag="PGRAT_RS04350"
                     /old_locus_tag="PGRAT_04545"
     CDS             2024..3763
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1068:
                     extracellular solute-binding protein family 5"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913517.1"
                     /locus_tag="PGRAT_RS04350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04545"
                     /product="peptide-binding protein"
                     /protein_id="WP_025705305.1"
                     /transl_table=11
                     /translation="MAKKRKWWSGLLVLSLVGVLFTGCSTNNNATKSPNATAESSASAA
                     SEAPAASEPAAGAPVDGGTLVMSTFSDIVNLNPLMINDTSSGDLAEFVFAKLYNLDREG
                     NVKAEPWSLAADVPEISADGLTYTVKLKDGIKWSDGQPVTADDVVFTVDMAKNPETGSP
                     LTSLFDKVKTVEKVDDKTVKFTMKQVYAPFLYSLVQAVVPSHVLKDVKPKEMQTNPYGT
                     DPAKTVTNGPWKWTSWKQGDSHTLDADPNYWGEVKPHIAQLVYKIYADQNTEVQALLKG
                     DTDHISAIPVTQVEAVKANKDIHIIQKPGAQYEYVMFNFDAKNFKDKYGLFTGQKTRQA
                     IAHALNRQGMVDNILKGVGAVMNAPFLPDTWADPGDAAVNYDYNAETAKKLLAEDGWVA
                     GKDGILTKDGHRFSFELQYNAGNSRREQVAAVIQQNLKEVGIEVTPKAIDFAAWIDQNV
                     TPGKFQALLLAWSLNTPDPDSESVFSSKYFPPAGQNSGWYKNEKLDQLWVDGYSTVDQA
                     KRKEIYKEVGKEISTDLPYVFLYQYGQAIGTGPRVHWAEEDAPEPSLGYGQYFHAIKWW
                     VTD"
     misc_feature    complement(2056..2074)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.63"
                     /tool="antismash"
     gene            3885..4856
                     /locus_tag="PGRAT_RS04355"
                     /old_locus_tag="PGRAT_04550"
     CDS             3885..4856
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1118:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728862.1"
                     /locus_tag="PGRAT_RS04355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04550"
                     /product="ABC transporter permease"
                     /protein_id="WP_025705306.1"
                     /transl_table=11
                     /translation="MTEYLIRRVLQSVLVIFLITILTFLLIHAAPGGPTQMMLSPGLSP
                     EVFKQQAHNLGLDQPVPVQYVRWISGLLQGDLGYTFKNHLPVADLLWPRIGNTFILMGA
                     AWLLSLIIAIPWGIYNSTKVYGLSDQSASFISYLGFAMPTFWFGILLQQWFSLKLRWFP
                     LSDMHTRGKEGDIGDLFMHLVLPVTVLSLGFLASYMKYARASMLEVLDQDYIRTARAKG
                     VKERRVVFHHALRNALIPIITILGLDLPILVAGAALTESVFNWPGMGRWFVQMATQREY
                     SVLMAITIVTAVMVVLGNLLADILYAIVDPRVKLGKQGGKAA"
     gene            4889..5833
                     /locus_tag="PGRAT_RS04360"
                     /old_locus_tag="PGRAT_04555"
     CDS             4889..5833
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1085:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913515.1"
                     /locus_tag="PGRAT_RS04360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04555"
                     /product="ABC transporter permease"
                     /protein_id="WP_042267808.1"
                     /transl_table=11
                     /translation="MSSTKTKKAAELKKPPGPWAILWKKFSHNPYAMGGLVVLLFFIVI
                     AALAPLLTKYKPEAIDLMFANLKPGVDGHLLGTDELGRDILTRLLYSARISLMIGFSVA
                     FASVAIGSIIGAISGYFGGWVDTVFMRIVDVMNSVPTLFLNILVLAIFGSRIEYMILIL
                     ALTSWMSIARLVRGNFLQLREMQYVEAAKAIGVSSWGIIFRHLLRNASFPIIVNATLMV
                     GGAILSESALSYLGLGIQAPATSWGLMLSNAQEFMLVDPLQAVYPGLCILLVVLAVNFI
                     GDGIRDGLDPRQQLTKSRRRLDKWRKNYSKSGN"
     gene            5800..6816
                     /locus_tag="PGRAT_RS04365"
                     /old_locus_tag="PGRAT_04560"
     CDS             5800..6816
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913514.1"
                     /locus_tag="PGRAT_RS04365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04560"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_025705308.1"
                     /transl_table=11
                     /translation="MAEKLLEIRKLTTGFVTEKGLVKATDQISIRVDKGQTVCLVGESG
                     SGKSVTSLAIMRLIDYAGGMILEGNINFHGQDLAVKKQEEMRDIRGNQIAMIFQDPMSS
                     LNPVFTVGEQIAESLQLHQNKSSKEAMQMAIDLLKLVGIPAPEIRARQYPHELSGGMCQ
                     RIVIAIALACNPELLIADEPTTALDVTVQAQILDLLRKLQAELGMSILLITHDMGVAAE
                     IADRIAVMYAGAIVEEGTVEEIFDHPSHPYTIGLLQSIPGFAGGRGGELYTIKGTIPPI
                     GQLPPGCRFHPRCPHAMEVCSMKEPPDFMITDDHRTACWLFQDKPVQADNKDGVRQA"
     gene            6813..7832
                     /locus_tag="PGRAT_RS04370"
                     /old_locus_tag="PGRAT_04565"
     CDS             6813..7832
                     /EC_number="7.4.2.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223365.1"
                     /locus_tag="PGRAT_RS04370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04565"
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="WP_025705309.1"
                     /transl_table=11
                     /translation="MTNETAAKPLNNTTSAEVLVEVKDIKKYFPITKGLLNRTVGQVKA
                     VDGVSLSIRRGETFGLVGESGCGKSTFGRVLLRLQSATGGEVLFGGRDIHSLGHQELRK
                     LREEMQIIFQDPFGSLNPRFLVKDIIGEPLKIHRNMTANEIDARVVELMELVGLDATRR
                     NRYPHEFSGGQRQRIGIARAIALNPQFIVADEAVSALDVSVQSQVINLLMKLQKELGLT
                     FLFIAHGLNVVRHISDRVGVMYLGKLVEVAETEALFAAPLHPYTAALLSAIPKPTPRRK
                     QDRIVLQGDVPSPANPPSGCRFHTRCPLAQERCRQQEPLLEELHPGRMVACHFPLASS"
     gene            8127..8780
                     /locus_tag="PGRAT_RS04375"
                     /old_locus_tag="PGRAT_04570"
     CDS             8127..8780
                     /EC_number="3.4.-.-"
                     /GO_function="GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913512.1"
                     /locus_tag="PGRAT_RS04375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04570"
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="WP_025705310.1"
                     /transl_table=11
                     /translation="MQIQLGDQTLQLHVQYAKRKKISLQIDMVGLITVKAPTGTSEEMI
                     RSAVQRHGEWILESLRRNAAARQLPAPKEYQGEGRFLYLGKEYLLHELINTGEQDAEKL
                     KLELKKFYFASLKKIAAERITRYQTMLKVKPKSIEIVESRTKWGSCSADKKLTFNYRLA
                     MAPPEVIDYVIIHELCHLLHMNHDRSFWRRLGSVMPDYKEKEAFLARQGQFMTL"
     gene            complement(8777..9652)
                     /locus_tag="PGRAT_RS04380"
                     /old_locus_tag="PGRAT_04575"
     CDS             complement(8777..9652)
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389367.1"
                     /locus_tag="PGRAT_RS04380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04575"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_025705311.1"
                     /transl_table=11
                     /translation="MQQLTLMKDYKHNDPLRKSLSGLAESTFGISFERWYREGFWSERY
                     IPFSYVQEDQVIANVSVTLLDWIINGVKKSAVQIGTVMTHPGYRHRGLSARLMHAVLAE
                     YGDSADFMYLFANDTVLDFYPRFGFIPEEEHLFSMDFSAPLSGAGGIRKLDINSLEDLA
                     FIAKFAEGRLPVSKRFGTGRTQGLLMFYCLEVFNEDIYYLENEDVIVIYKNEGKQLELF
                     DIISHREINLEEILTKLAGRDTEKVLFHFTPDYEGIPVTSTPHHSGLFIRAGGDNIFPH
                     HVKHPATSIA"
     misc_feature    complement(9786..9806)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 21.92"
                     /tool="antismash"
     misc_feature    complement(9931..9947)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 21.52"
                     /tool="antismash"
     gene            10001..11929
                     /locus_tag="PGRAT_RS04385"
                     /old_locus_tag="PGRAT_04580"
     CDS             10001..11929
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913511.1"
                     /locus_tag="PGRAT_RS04385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04580"
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_025705312.1"
                     /sec_met_domain="GATase_7 (E-value: 1.1e-15, bitscore:
                     56.1, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 2.3e-27, bitscore:
                     95.1, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAIAGIYHLQHQPLDPKQGPDLMQQLSRYPADEIRTWQSTDVFL
                     GCLSQWITPESLGETLPYYDSLRKLAITADAIIDNREELFERLQVPRGDRVSMPDSLLI
                     LLAYEKWGEQAPVYLVGDFAFMIWDERKRALFGARDFSGNRTLYFLRSRGLLSFCTVIH
                     PLLSMPGADRTLNEGWASEFLAIHTRTDVSDQYSTVYQSIEQLPPSHSISVKDGNIFFS
                     RYCTLQAPPELRLRSDQEYEEAFREVFGQAVRDRLRTHRAVGANLSGGLDSGSVVSFAA
                     RELRRQGQPLHTFSSYPVDHFVDFNIGKRIADERPYIQETIDYVGNVRPNFLNFPDLSP
                     YSEIDDWLDIMEMPYKFIENSYWLKGIFEQAQMKNIGVLLSGQRGNWTISWGPALDYQA
                     KLIREFRWISFYRENRKYNESMGANRRKVFQIVGKKAFPSLTGLLPAKHEAPPDLIHPD
                     FARRTEVDERLKALGINSGSSQTIYDIRRKHFEQPHVWNVNGTIATKLSLKYRVWDRDP
                     TNDLRVIRFCLSVPESQYVQNGVDRSLIRRAMKGLLPDEVRLNRKRRGVQGADGIYRML
                     PKWDDFLQELREMIQDPRTSAYLNNTGLAGCLNRLGSEPAPNMIFNSDFRLLTRGLVFY
                     RFLKRLS"
     gene            11959..12084
                     /locus_tag="PGRAT_RS32995"
                     /old_locus_tag="PGRAT_04585"
     CDS             11959..12084
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002172970.1"
                     /locus_tag="PGRAT_RS32995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04585"
                     /product="paeninodin family lasso peptide"
                     /protein_id="WP_020426161.1"
                     /transl_table=11
                     /translation="MKKEYSKPALEVLDVKMTMAGPGVAIVDAFQDDPDEVQHYS"
     CDS_motif       11959..12018
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MKKEYSKPALEVLDVKMTMA"
                     /locus_tag="PGRAT_RS32995_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       12019..12084
                     /prepeptide="core"
                     /locus_tag="PGRAT_RS32995_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="-12.40"
                     /molecular_weight="2241.3"
                     /monoisotopic_mass="2240.0"
                     /core_sequence="GPGVAIVDAFQDDPDEVQHYS"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MKKEYSKPALEVLDVKMTMA"
                     /leader_location="[11958:12018](+)"
     misc_feature    11993..12007
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 20.44"
                     /tool="antismash"
     gene            12199..13140
                     /locus_tag="PGRAT_RS04395"
                     /old_locus_tag="PGRAT_04590"
     CDS             12199..13140
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PGRAT_RS04395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PGRAT_04590"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_025705313.1"
                     /transl_table=11
                     /translation="MASTVQGLAYKAFGLQIHSEFPLPELPLTGEPGLIGSVSVIQGDL
                     KERWEAMPKITPSLGVSGHEVMFEAKDTALFAIQDGNTITVSPAPGADPDCIRLFILGS
                     CMGILLMQKQILPLHGSALVIEGKAYALVGRSGAGKSTLASYLMDQGNPLISDDVIPVM
                     VHNGYPFAVPGYPQQKLWQQSLDYLGMNSSLYRPLFERETKFAVPVHDRFHGEPVQLAG
                     LFELSAAPEAPVSLQPIQGMERFHTLYNHTYQRAIVDRIGIREWHFGMLASFVGRLPMY
                     RLTRPEQGFSAPQQTSLILEALQQQLVKEMNS"
     gene            13137..13442
                     /locus_tag="PGRAT_RS04400"
                     /old_locus_tag="PGRAT_04595"
     CDS             13137..13442
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913504.1"
                     /locus_tag="PGRAT_RS04400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04595"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_025705314.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 8.9e-24,
                     bitscore: 81.8, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 9.5e-14, bitscore: 50.0,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNMNTADVLSLESVLVQREGNIASDMDGEKVMLNVKNGKYYNLGE
                     VGGEIWTALASPVTAGRVVEIIRETFEVPEEIARQDVFVFLQSLLDEDLAGIVSQP"
     aSDomain        13182..13421
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_PGRAT_RS04400_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="4.10E-26"
                     /identifier="RREFam002.1"
                     /label="PGRAT_RS04400"
                     /locus_tag="PGRAT_RS04400"
                     /protein_end="95"
                     /protein_start="15"
                     /score="81.8"
                     /tool="antismash"
                     /translation="VQREGNIASDMDGEKVMLNVKNGKYYNLGEVGGEIWTALASPVTA
                     GRVVEIIRETFEVPEEIARQDVFVFLQSLLDEDLA"
     gene            13439..13897
                     /locus_tag="PGRAT_RS04405"
                     /old_locus_tag="PGRAT_04600"
     CDS             13439..13897
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913503.1"
                     /locus_tag="PGRAT_RS04405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04600"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_025705315.1"
                     /sec_met_domain="PF13471 (E-value: 9.1e-26, bitscore: 88.7,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MITLRRIRLLLTVDKAALALLPEALWRLFLVRIQLLFPFAKTAPQ
                     LGLQAQETPTLSKEGDIARIQHVTKAIRVMSRVTPWKSTCMVRAVAGLKMLEKRGIEST
                     LYMGVAKDKQGQMIAHAWLRSGSYYVSGDDAMKGFVVVEKFAKVLHAD"
     gene            13911..15710
                     /locus_tag="PGRAT_RS04410"
                     /old_locus_tag="PGRAT_04605"
     CDS             13911..15710
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913502.1"
                     /locus_tag="PGRAT_RS04410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04605"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_025705316.1"
                     /transl_table=11
                     /translation="MQAIMYYIRQLQRLSGVKLYLNLLGMVISGLLESSAVLLLVPMLG
                     MAGIQLGGNAAGIDLPMFDFISELPQTSALALVLGAYVLIVLCQNLISRFVAIRNVDIQ
                     QSYSRKLRYEVYGALLRAEWSFFLKKRTSDLINVMTTELARVLAGISSFLQFLTSLLFT
                     LVQIGFAFWLSPQMTLAVLVCAVLLSLFSRRFIRKAKRLGSRSSELGKQYLAGITDQLN
                     GIKDIKSNNLEDSRLSWMHEFTGEVKSEQLDYTRLRTNSQLLYKMSSTLLIAVFIFVSF
                     RFLHVEGPYLFLVILVFARLWPTFSNLQTLLEQLASVLPAFKQLQLLHEESLQAAEHGA
                     HAQSGTVAAPMTLKRSLEARDVNFRYNPQEENYSLRSVDVVIPANRMTAIVGRSGAGKS
                     TLVDLLMGLIQPESGEILADGEPVTGERLLPYRRSIGYVAQDPFLYNATIRDNLTMIKP
                     DASEAELWEALEFASSADFVRKLPQGLDTLLGDRGVRLSGGERQRLVLARAIIRKPSIL
                     VLDEATSALDTENEKRIQEALERLKATVTVIVIAHRLSTIRGADQILVIDQGRVIQQGV
                     YSGLASEQGGMFSRLLSSQEEAM"
     gene            complement(16423..17607)
                     /locus_tag="PGRAT_RS04415"
                     /old_locus_tag="PGRAT_04615"
     CDS             complement(16423..17607)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /locus_tag="PGRAT_RS04415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04615"
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_042266087.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 1.1e-61, bitscore:
                     207.0, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDGYALDKKGFSKELRLLLEVLKTDDIDETAERNPQLFTDIDWE
                     LWVRLSLHHRVFPYIYPKLSRMREEHVPDAVTQRLKWEYRKNTVQMLQLSGEMGYLGEE
                     LAKLQIRSLFLKGPVLAQELYGDISLRTSRDLDFLVPMAQLAETEALLVRLGYVKEEDF
                     ESILGDWKWREHHTTFNHPDKGIKVEVHWRLSPGPSKEPEFDELWKCARTSSHFGQNVH
                     YLGSEDLFLFLAAHGARHGWSRLRWLLDIRQLLLQKPDSGKLAGLIQQYNYADVGGQTL
                     LLAADLLAAPVEPALEGFTKRPKARRIAQLALFYVARMINLHNPPLEPEAERHYKYYQP
                     AILTRWHQFLYVAGFLYPYAVDAKTLPLPKPLHVLYFPLRPFLWTWRKVSGQEK"
     gene            17873..18745
                     /locus_tag="PGRAT_RS34190"
                     /old_locus_tag="PGRAT_04620"
     CDS             17873..18745
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018400.4"
                     /locus_tag="PGRAT_RS34190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04620"
                     /product="DUF1186 domain-containing protein"
                     /protein_id="WP_025708739.1"
                     /transl_table=11
                     /translation="MQHLIESIKYNKDRRFPREELEEIISRKEEAIPHLLEIMRELQAH
                     PQLAEDPARLDFMYAAYLLSQFHVTALFPILVELFSLPEELLDMIFSDILTDAGGRMLA
                     SVYDGDLHLLKGLIENREAGEYARAQGLRALTILVYEGRILRETAVGYVKELLTSKRDD
                     YDYYFNAEMVCTAVDLYPEEVYAEIEKLYEEEALDPYVILLDEVQATLQMSQAVAMQHK
                     HEQVSYQYINDIHAEMEKWVCFQPESSGKIELLGDQLKQKSVKVDPKPGRNDPCPCGSG
                     KKYKKCCGK"
     gene            18855..20090
                     /locus_tag="PGRAT_RS04425"
                     /old_locus_tag="PGRAT_04625"
     CDS             18855..20090
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010283041.1"
                     /locus_tag="PGRAT_RS04425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04625"
                     /product="MFS transporter"
                     /protein_id="WP_042266090.1"
                     /transl_table=11
                     /translation="MPSNWKKNIVLFLTSQTISLFGSSLVQYAILWYITLNTQSGLMMT
                     ISIICGFVPTFLLSPFAGVWADRYSRKLLIVLSDTCIAAATLVLALLFLMGYDEMWLLF
                     VVSAVRALGTAVQTPAVGAMLPQLVPEEQLTKVNGTNGSIQALIMLLSPMLSGALLTVA
                     SIEVIFFIDVITAALAVAAMLLFVKVPLHAKAAAEQTLSYFSDMRLGFSYIKQHSYIRT
                     FFIFMAFFLVLAAPVAFLTPLQVTRTFGDDIWRLTAIEIAFSIGMMLGGLILAGWQGFR
                     NRVYTMTMSSLAMGAFTFALGVVPVFWIYLLFMGLVGLAMPFFNTPSTVLLQEKVEPDY
                     LGRVFGVLGMISSSMMPLGMLIFGPLADVVKIEWMLMLTGLLMFIQGFFLLGSKVLVKA
                     GEPAPAEADVKQ"
     gene            20117..21040
                     /locus_tag="PGRAT_RS04430"
                     /old_locus_tag="PGRAT_04630"
     CDS             20117..21040
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /locus_tag="PGRAT_RS04430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04630"
                     /product="phosphotransferase"
                     /protein_id="WP_036703872.1"
                     /sec_met_domain="APH (E-value: 5.9e-28, bitscore: 96.9,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFVSGIPGAESWTTVEPVLKGWSSDRKFYVEDQAGRRLLLRLSA
                     GRTFQRKQQEYEAIQRFYRLGFPMSRAVDFGIYGEGEGGEQYVYMLLTWVDGVPLEERL
                     PGLPPEEQYRLGAEAGRILKQMHTIPVQEELPHWEADMQSTILSRIREYENCPYRLEDD
                     QQVIAFVRQNVGLIHNVEKVHHHGDFHVGNLIYTPEGRIGVIDFNRWDIGDYAEEFYKL
                     QIFDRERSIPFAAGKLEGYFGGPPPEAFWKRQALYVAYASLYSIKWSIPYGAADVHDMM
                     DRYRLALSDYDHFRRTVPGWYLGDGE"
     misc_feature    21087..21103
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.52"
                     /tool="antismash"
     gene            21173..21613
                     /locus_tag="PGRAT_RS04435"
                     /old_locus_tag="PGRAT_04635"
     CDS             21173..21613
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913497.1"
                     /locus_tag="PGRAT_RS04435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04635"
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="WP_025704412.1"
                     /transl_table=11
                     /translation="MNNEELNNGLPENYEELKKAAGRSADWRSRLEAVEELGRFNHKQI
                     IDILNRLMVSDPVYTVQEAAYEKLKAFGEDVTVPSKNKAELFRGISKIVLRIKKSLPQD
                     HSYEEFKEKLKKMRIDVYDAYEGEKGENFDAWLQKLWASATK"
     gene            21704..22300
                     /locus_tag="PGRAT_RS04440"
                     /old_locus_tag="PGRAT_04640"
     CDS             21704..22300
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PGRAT_RS04440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PGRAT_04640"
                     /product="hypothetical protein"
                     /protein_id="WP_025704413.1"
                     /transl_table=11
                     /translation="MQNSSANEPLTRLFQEYLELSAHLRPDYTASLGSGHREGQPAISD
                     IVPDAPKLLQAIYSQVSGTSSDEEEPSLIEFIPGYRLIHIQEYVQEIKVLESILKEKGC
                     SGGGTVLPILTNYGSDFICYYQSEDGTERICDLLHDYGDLAVMYDSPEKFLETLCEFYK
                     QEVYFLDEEGYLDCDLVLEGEVGAALNPSANYWSE"
     gene            22369..23256
                     /locus_tag="PGRAT_RS04445"
                     /old_locus_tag="PGRAT_04645"
     CDS             22369..23256
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /locus_tag="PGRAT_RS04445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PGRAT_04645"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_025704414.1"
                     /sec_met_domain="Abi (E-value: 4.5e-16, bitscore: 57.5,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHYIFRNRTRQLRAGWEILLAAAAIFILTMLMSFGLSLRVNSRQL
                     FDPDGWVRVFKASGLYMLVVVCFTVRVVRRLPLSSIGLSRPDVKRFAQGFLSGALLMAI
                     IVMVLWALGNAAFQNEGWKPRFSQLDLPGLMLTACIAGICEEVLFRGYIQHLLSGRLGT
                     TGAVAITSVLFSAAHMANPGYNWISALNIVLIALIFSCATLRTGNLYFAMALHLSWNLV
                     QGYGFGVSVSGKIPQGLYSVLLGGPDWITGGRFGLEASILTTILLGLICAGLLLVHVMK
                     PRRRTQQTEFSSRY"
ORIGIN
        1 tgaccgttat tgccggttat tttgtgttcg ggcttggcag ggacagggaa cgttaacagg
       61 gaataccatg aaattcaata ataaatcccg attgactggt tcgctttatt tagcagctca
      121 atacaaccga tattaatatg gagccttaga aaactccatg aaaggaatta ttgagcttga
      181 agactacgat gaaagcagcc ttttccagaa cattgccatt tttagtggtg gcagcagcct
      241 actttttgta cacaaaaatc aatacaatgt ccaatccgca ggtagaggcc ataggtttat
      301 caccgtatgc gatttttttt gtaggggccg tattatcctg gaaatttaac cggagccggg
      361 aattttacat tctgctgatt cttgcgattt gtatagcgtc catggcctat ttgccggaga
      421 tttcaggtac agctctgcgg agtggggata tttattccat tatctgtctg gcgattccgt
      481 tgaacattgc gctgttttcc ttttttaagg agaggggcat catcagctta tggggaagcc
      541 tccggatggc tctgatcctg gggcaatgcc tgtttctgtt ctggcaggtg aagtcgggag
      601 aaagagaatg gcttcatctc ttgaataaag aggtagtacc tgtagatctc cacaccgtaa
      661 ctcccgtacc gcagctgtct gtgattgcgt ttgtggtcac ctttgtgatc ctgatagtca
      721 ggggaattgt ataccgttcc tcttcgcagg agatttcatt cataagtgtc ttattagcca
      781 tattttacgt gctgcaccag aacaatagtc cgctgctgta ttcgatcatt tttgctgctt
      841 cagggcttgt tttcattatt tcgatcattc aggattcgta ctcgatggct ttttccgatg
      901 agctaacggg tctgccttcc cggcgggcgc ttaagcagga catgatgaag ctgggcttta
      961 actatgccat cgctatgctg gacattgatt ttttcaaaaa attcaatgat acctatggac
     1021 atgacacggg agatgaagtg ctgagactgg tggcttccaa cataaaggag gttacgggcg
     1081 gagggagagc cttccgctat ggcggggagg agtttactat tctttttccg ggtaaaaaca
     1141 taaatgatgt gcttccccat ctcgaagaac tgcgggagaa aatatccaaa caggccttca
     1201 ccatacgcgc caaaggcaga tccaaaaaca agtcttcaaa aaggtcctcc agtgccagga
     1261 cctccaagca aatatacata accgtaagca tcggtgtctc ccaaaagaat gagaagtaca
     1321 aaaccacaga aagcgtaatg aaatcggcag acactgcttt ataccgcgcg aagaaaaagg
     1381 gcaggaattg tgtgagcaaa taaaagggga tacggcaatg gaagataacc gctataaact
     1441 agactttgca aagctatacc agtagaagca tacatattat taagaagcga acagcagaga
     1501 aggtagagat accctagcgg ctgttcgctt cttttttatg cgtaatatga atatatatat
     1561 accaataaaa atatcacatg taattaacat ggttaaatca aacggctgtt atcaaaactc
     1621 tctttatttt aacttttaat ggtattaatg attgagcccg gaaatacatt ggaatgaaaa
     1681 gggattatac tacattatat tgaaataaga caaggtagaa aacgcataaa aaagatattg
     1741 acgtaagaaa atttaacatg taatataaac cataatttat atgggaaagc tagtttcccg
     1801 acatgagttg tttctgttgg taattgacct ttggtctatt tcaggggatg acgacttaat
     1861 agtaaaaggt ttttttgtga ggtattgtta cataccttga aggctgttgg actgttttga
     1921 tttgcacaag ggaggttgaa agcttcagca gagagatcat gatagaaccc ggcaacgctg
     1981 gcggttcacc gtacacgaac ctaactaagg aggagtaaat cgaatggcaa aaaagagaaa
     2041 atggtggtct gggttactgg ttttatctct agtaggggtt ttatttacag gttgcagtac
     2101 aaataacaac gcaactaaat cacctaatgc tacggcagag agctctgctt cagccgcaag
     2161 cgaagctccc gcagcttcag aaccggctgc aggcgcccct gttgatggcg ggacgctggt
     2221 tatgagcaca ttctccgata tcgtcaatct taatcctctt atgatcaacg atacttcttc
     2281 cggtgatttg gcagaattcg tatttgccaa actttacaat ttggaccgcg aaggaaatgt
     2341 aaaggcagaa ccttggtctc tggcagcgga tgtgccggaa atctctgcgg atggattaac
     2401 ttatactgtg aagttgaaag acggcatcaa atggagcgat ggccagccgg tcactgcaga
     2461 cgatgtggta tttacggttg atatggcgaa gaatccggaa accggatcgc cgcttacaag
     2521 tctattcgac aaggtaaaga ccgttgagaa agtcgatgac aagacagtga agttcacaat
     2581 gaagcaagtg tacgcgccat tcttgtactc cttggttcaa gctgtagtac cttcccatgt
     2641 tctgaaggat gtcaagccta aagaaatgca aactaatcca tatggtaccg acccggccaa
     2701 aaccgttaca aacggaccct ggaagtggac ctcctggaaa cagggcgaca gccataccct
     2761 tgatgctgat ccaaactatt ggggcgaagt taaacctcac attgcacaat tggtttataa
     2821 aatctatgct gaccagaata ccgaggttca agctctcctg aagggcgaca cggaccatat
     2881 cagtgctatt ccggttacac aggttgaggc agtcaaagcc aacaaagaca ttcatatcat
     2941 tcaaaagccg ggcgcccagt atgaatatgt aatgttcaac ttcgatgcca agaacttcaa
     3001 agacaaatac ggtttattca cgggacagaa gaccagacag gccattgctc atgcactgaa
     3061 ccgtcaaggg atggtcgata acattctgaa gggtgtcggt gcggtgatga atgcaccgtt
     3121 cctgccggat acctgggctg atcctggcga tgcagccgtt aactatgatt acaatgcaga
     3181 aacggccaag aaactgttgg cggaagacgg atgggttgcc gggaaagatg gcatcctgac
     3241 caaagacgga caccggttct ccttcgaact gcaatataat gcgggcaaca gccgccgtga
     3301 gcaggttgct gcagtcattc agcagaacct gaaggaagtc ggcattgaag ttacaccgaa
     3361 ggccattgac tttgcggcct ggattgacca gaacgtaacg ccgggtaaat tccaagccct
     3421 gctgttagcg tggtccctga acactcctga cccggattca gagagtgtct tctcctccaa
     3481 gtacttcccg cctgccggac agaacagcgg ctggtataag aatgagaagc tggatcagct
     3541 gtgggtagac ggatattcta cggttgacca ggccaagcgt aaagaaattt ataaagaagt
     3601 aggtaaagaa atctctaccg acctgcctta cgtgttcctg tatcaatatg ggcaagccat
     3661 tggtacgggg cctagagttc actgggcaga ggaagacgct cctgaacctt cactgggtta
     3721 tggacagtac ttccatgcga tcaaatggtg ggtaaccgac taattgaaca gacacaggaa
     3781 atagaattgc gggggaggaa agagaattct ctttcctctt ctccttttgt catgaaggag
     3841 gcccgtgatc ctatgcctta ccatctctag gaggacgact tttgatgaca gaatatctga
     3901 tccgccgcgt actgcaatcg gtgctggtga tctttttgat tactatactt acctttcttc
     3961 taattcatgc ggctcccgga gggccgaccc agatgatgct gtctccggga ctttcaccgg
     4021 aagtcttcaa gcagcaggcg cataatctcg gcctggacca gcctgtccct gtgcagtatg
     4081 tgcgctggat tagcggcttg ctgcagggag acctcggtta tacttttaaa aatcatctgc
     4141 cggttgcgga tttgctatgg ccccgtatcg gcaatacctt cattctcatg ggagcagcct
     4201 ggctgttatc tctaatcatt gcaattccct gggggattta taacagcacc aaagtatatg
     4261 gattgtccga tcagtcggcc tccttcatct cttatctcgg ctttgcgatg ccgacgttct
     4321 ggttcggaat cctgttgcag cagtggtttt ccctgaagct gcgctggttt cctctgtccg
     4381 atatgcatac aaggggcaaa gaaggggata tcggcgactt gttcatgcac cttgtgctgc
     4441 cggtaacggt gctgtcactg ggttttcttg catcctatat gaagtatgca cgcgcaagca
     4501 tgcttgaagt gctggatcag gattatatcc gtaccgcacg cgccaagggt gtcaaagaac
     4561 gcagggttgt cttccaccat gcgctgcgga acgcgcttat tccgattatc accattctgg
     4621 ggctggatct tcctattctg gtggccgggg ccgcgctaac ggagagtgtg ttcaactggc
     4681 cgggtatggg acgctggttc gtacaaatgg cgacccagcg cgagtattct gtactcatgg
     4741 ctataactat cgtaacggcg gttatggttg tgctgggcaa tctgcttgcg gatattttgt
     4801 atgcgatagt agatccccgc gtcaagctcg gcaagcaggg aggtaaagca gcatgagtac
     4861 taatccttca ccattgcccg ctgatgccat gtcttcaacg aaaacgaaaa aagcggctga
     4921 attgaaaaaa cctccgggtc cctgggccat cttgtggaaa aagttctcgc acaaccccta
     4981 cgccatgggc ggacttgtcg tgctgctttt tttcatagtg attgctgcac tcgctcctct
     5041 tctcactaag tataagccgg aggccattga tttgatgttt gccaacctga agcctggagt
     5101 ggacgggcat ctgctgggaa cagacgagct gggccgggac atactcacca gactgctcta
     5161 cagtgcgaga atctccctga tgatcgggtt ttcagtagcc tttgcttcag tggctatcgg
     5221 ttcgattatc ggtgcgattt caggctattt tggcggctgg gtggataccg tcttcatgcg
     5281 tattgtggac gtgatgaact ccgttcctac gctgttcctg aatatactcg tgctggcgat
     5341 attcggctcc aggattgagt atatgatcct gattctggcc ttgaccagtt ggatgagtat
     5401 tgcgcggctg gtgcggggga acttcctgca gcttagggaa atgcaatatg tcgaggcggc
     5461 caaagcgatc ggggtatcga gctgggggat tattttccgg catctgctcc gaaatgcaag
     5521 cttcccgatt attgtgaatg cgactctgat ggttggcggt gcgattctaa gtgaatcggc
     5581 attgtcctat ctgggccttg gcatccaggc tccggccaca agctggggac tgatgctcag
     5641 caacgcgcag gagttcatgc tggtagatcc gctgcaggcc gtatatcccg gtctgtgcat
     5701 cctgctggtg gtgcttgcgg ttaactttat cggcgatggt atccgggatg gactggatcc
     5761 cagacagcaa ttgaccaaat cccggaggag gctggacaaa tggcggaaaa attactcgaa
     5821 atccggaaac tgaccaccgg ctttgtaacg gaaaaggggc tcgtgaaggc gaccgaccaa
     5881 atctccatcc gtgtggataa agggcagacg gtgtgtcttg tcggtgagtc cggcagcgga
     5941 aaaagtgtga cctcgcttgc cataatgcgg ctgattgatt acgccggcgg aatgattctt
     6001 gaaggcaata ttaactttca tggacaggat ctggctgtga aaaagcagga agagatgcgg
     6061 gatatccgcg gcaatcagat tgcgatgatt tttcaggacc cgatgtcctc gctgaatccg
     6121 gtattcacgg ttggcgagca gatcgccgaa agtctgcagc tgcaccagaa taaaagttcc
     6181 aaagaggcta tgcaaatggc cattgatctg ctgaagctgg tcgggattcc cgcaccggag
     6241 atccgggcaa gacagtatcc gcatgagctc tccggcggga tgtgccagcg gattgttatt
     6301 gctattgctc tggcctgcaa tcccgagctg ctgatcgccg atgagccgac aactgccctt
     6361 gatgttacgg ttcaggcgca gattctggat ttgctgcgca agcttcaggc ggagctaggc
     6421 atgtccattc tgctgatcac ccatgatatg ggcgtggctg ccgagattgc ggaccgcatc
     6481 gctgttatgt atgcgggagc tattgtagaa gaaggtacag tagaagagat ttttgatcat
     6541 cccagccatc cgtatacgat tggcctgctg caatccattc ccggctttgc gggagggcgt
     6601 ggcggagaac tgtatactat taaaggaacg attccgccca tcggccagct tccgccgggc
     6661 tgccgctttc atccgcgctg tccgcatgcg atggaggtct gcagcatgaa ggagccgccg
     6721 gatttcatga ttacggatga tcaccgtacc gcctgctggc tgtttcagga taagccggtc
     6781 caagctgaca acaaagatgg ggtgagacag gcatgaccaa tgagacagca gccaaaccgt
     6841 taaataatac aacctccgcc gaagttttgg tggaagtgaa ggatatcaaa aaatatttcc
     6901 cgatcaccaa aggcctgctt aaccggacgg ttggccaggt gaaggccgtt gatggtgttt
     6961 ctctgtctat tcgcagaggg gaaacgtttg gcctggttgg tgaatccggc tgcggcaaat
     7021 ccacttttgg acgggttctg ctccggctgc aaagcgccac cgggggggag gtgctgtttg
     7081 gcggccggga tatccattcg ctgggccatc aggagctcag gaagctgcgt gaggagatgc
     7141 agattatttt tcaggacccg ttcggttctt tgaatccgcg gtttctggtt aaggatatta
     7201 ttggcgagcc gctgaagatt caccggaaca tgacggcaaa tgagatcgat gccagagttg
     7261 tggagctgat ggagctggtt ggtctggatg ctacgcggag aaaccgctac ccgcatgaat
     7321 tctcgggcgg acaacgccag cgcataggga tcgcgcgtgc gattgcgctg aatccgcagt
     7381 tcattgtcgc cgacgaagca gtatccgcgc tcgacgtatc cgtgcagtcc caagtgatta
     7441 acctgctgat gaagctgcag aaggagcttg ggctgacgtt tctgttcatt gcgcatgggc
     7501 tgaatgtcgt ccgccatatc tcggaccgtg taggcgtcat gtatctgggc aagctggttg
     7561 aggtcgcgga gacggaggcg ttgtttgccg ccccgctgca tccgtatacg gcagcgctgc
     7621 tctcggcgat tcccaagccg acgccaagac ggaagcagga ccggattgta ctgcaaggtg
     7681 atgttccttc accggcgaat ccgccgtccg gctgccggtt tcatacccgt tgtccgctgg
     7741 cccaggagag atgccgccag caggagccgc tgcttgaaga actgcatccc ggacgaatgg
     7801 tggcctgcca ttttccgctg gcatcatcgt agtaccggtt tactctatct gaagcataaa
     7861 taatgtgaaa aagggatgct cctgaagccg ctggccggct gcgggagcat ccctttttgg
     7921 ccgtttctac ttctacagcg gaaaccggaa gtgtacagca gttgttaacc taagtgaaat
     7981 tccgggccgt tttaaaagta agcaaacctg cgttcggccg cgcccttgaa ttggctggag
     8041 ctttcttagg ggctgggcta tttggcatcg gtgaatccgt gttaaactta agggaattca
     8101 tcaaccttac agaaacgatg gtggctatgc agattcaact cggggatcag accctgcagc
     8161 ttcatgttca atatgccaaa cggaaaaaaa tctccctgca gatcgatatg gtagggctta
     8221 ttacagtcaa ggcgccaacg ggtacaagcg aagagatgat ccgcagcgca gtacagcggc
     8281 atggtgaatg gattctggag agcctgcgca ggaatgcggc ggcccggcag ctcccggctc
     8341 ccaaggaata ccagggggag ggcaggttcc tgtaccttgg gaaggagtat ctgctgcacg
     8401 aattaattaa taccggtgag caggatgcgg agaagctgaa gcttgagctg aaaaagtttt
     8461 attttgccag cctgaaaaag attgccgccg agcggatcac ccgttatcaa acgatgctga
     8521 aggtgaagcc caaatccatt gaaatcgttg aatcccgaac caaatggggg agctgcagtg
     8581 cggataaaaa gctgaccttc aattaccggc tcgccatggc gccgcctgag gtcatcgatt
     8641 atgtaatcat tcatgagctg tgtcatctgc tgcatatgaa tcatgaccgg tccttttggc
     8701 gccgtctcgg aagtgtgatg ccggactaca aggagaagga agcttttctc gcccggcagg
     8761 ggcagtttat gacgctttaa gcaatggatg tagccgggtg cttaacgtga tgagggaata
     8821 tattatcacc gcccgcccgt atgaataagc cgctgtgatg cggggtgctt gtgacaggaa
     8881 tcccttcgta atccggagta aagtggaaaa gtactttttc agtatccctg cctgccagct
     8941 tagttagaat ctcttcaagg ttgatctctc tgtggctaat gatatcaaaa agctccagct
     9001 gtttgccttc gtttttatat ataacgataa cgtcctcgtt ctccagataa taaatgtctt
     9061 cattaaagac ctccaagcaa taaaacataa gcaagccttg cgtacgtccg gttccgaagc
     9121 gcttggatac cgggagcctg ccctctgcaa atttggcaat gaaggctaaa tcctcaaggc
     9181 tatttatatc taattttcga atcccccctg cccccgaaag gggggcgcta aagtccatac
     9241 tgaaaagatg ctcctcttct ggaataaaac caaaccgtgg atagaaatcg agaaccgtat
     9301 cgttggcgaa aaggtacata aagtccgcgc tgtccccata ctctgccagc acagcatgca
     9361 ttaaccgggc ggataagccc ctatgccggt aaccgggatg tgtcatcact gtaccgattt
     9421 gcacggcgct tttcttcacg ccattaataa tccaatcaag cagggtcacc gatacattgg
     9481 caattacctg atcttcctga acgtaagaaa aagggatata ccgctcactc cagaagcctt
     9541 cccggtacca gcgttcaaag cttatgccaa aagtgctttc ggcaagcccg gacaggcttt
     9601 tacggagggg gtcattatgt ttgtaatcct tcattaaggt tagctgctgc atgtaaccgc
     9661 tccttagtaa atattttttc gtgtggggct aaaataactt gtgacgctta gctacccgtg
     9721 taattggctc catctgatct ggttccatgg tctcaattcc tggaaagggt cttcaattca
     9781 atagattata gtctttctgc ctatgttcat aacaaatata aaatatattg tcaatacgct
     9841 aaaggatacc aaaaagtagt cggtggtgtt attcacaatt aatgggaagg gcgttatctt
     9901 atatataaag aaacgttctt aattaagaac cagtaaaaaa gctccaatcg gtgctttcca
     9961 gaagcacgag ggaggctttt gaaccaggtg ggggcttgcc atgagcgcga ttgcgggaat
    10021 ctatcattta cagcatcaac ctctggaccc taagcagggg cctgatctta tgcagcagct
    10081 cagccgctat ccggccgatg agatcaggac atggcagagc acagatgtat tcctgggctg
    10141 tctgtcgcag tggattacgc cggaatccct tggggagacg cttccttatt atgattccct
    10201 gcggaagctg gccattacgg ctgacgccat tattgataac cgtgaagagt tgttcgagcg
    10261 gcttcaggtg ccgcgcgggg atagagtctc tatgccggac agtctgctta ttctgctggc
    10321 ttatgagaaa tggggcgagc aggcaccggt ttatcttgtc ggtgactttg cctttatgat
    10381 ctgggatgaa cggaagcggg cactgttcgg ggccagagat ttctccggga accggacgtt
    10441 gtacttcctg cggtccaggg ggctgttatc tttctgtacc gtcattcatc cgctcctgtc
    10501 gatgccgggg gcagaccgga ctctgaatga gggttgggcc tccgagttcc tggcgattca
    10561 tacacggacc gatgtttcgg accagtattc cacagtctat cagtccatcg aacaactgcc
    10621 gccgtcccat tctatttccg taaaggacgg gaatatcttc ttctcccggt actgcaccct
    10681 gcaggctcca cccgagctgc ggctgcggtc agaccaggag tacgaggaag cgtttcgtga
    10741 ggtgttcggg caagcggtcc gggacaggct ccggacgcat cgcgcggttg gagccaacct
    10801 aagcggaggc ctcgattccg gatcggtagt cagcttcgcc gctagagagc ttcgccgcca
    10861 gggccagccg ctgcatacct tcagttccta tccggtggat cactttgtcg atttcaacat
    10921 cggcaaacgt atcgccgacg agcgtcccta tatccaggaa accatcgatt atgtcgggaa
    10981 tgtccggcct aactttctga actttcccga tctgagtccg tacagtgaaa ttgatgactg
    11041 gctggatatt atggagatgc cttataaatt tatcgagaat tcctattggc tcaaagggat
    11101 ctttgagcag gcgcaaatga agaacatcgg cgtgctgctc agcgggcaga gaggcaactg
    11161 gaccatttcc tggggtcctg cactggatta tcaggctaag ctgattcgtg aattccgctg
    11221 gatctccttt taccgggaaa accggaagta caatgagtcg atgggggcaa accgcagaaa
    11281 ggtatttcaa attgtcggca aaaaagcctt cccgtcgctg actggactgc tgccggctaa
    11341 gcacgaagct ccgcctgatc tcatccaccc tgatttcgca aggcgcacag aagtggacga
    11401 acggcttaag gcgctgggga ttaacagtgg ctcttcccag accatctacg atatccggcg
    11461 caagcatttt gaacagccgc atgtctggaa tgtgaacggg acgatagcaa cgaagctgtc
    11521 actgaaatac cgggtgtggg atcgtgatcc tacgaatgat ctgcgggtta tccgcttctg
    11581 cctgtccgtg ccggaaagcc agtatgtgca aaatggggtg gaccgctcgc tgatccgcag
    11641 agcgatgaaa ggactgctgc cggacgaggt gcgtttgaac cggaaacggc gcggcgttca
    11701 gggggcggac gggatttacc gtatgctacc gaagtgggac gattttcttc aggagctcag
    11761 agagatgatc caggaccccc gcacctctgc ttacctcaat aataccgggc tcgccggatg
    11821 tttgaaccgg ctgggtagtg agcccgcacc aaatatgatt ttcaactcgg attttcgcct
    11881 tctgacacgt ggacttgtgt tttaccgttt tctgaagcgt ttatcctgat gagggaatgc
    11941 ttgaaagggg gtgaaaatat gaaaaaggaa tacagcaagc ctgctttgga agtactcgat
    12001 gtgaaaatga ctatggctgg acctggtgtt gctatcgtcg acgcgttcca agatgatccg
    12061 gatgaagtac aacactactc ctaggatggt tttgttcgcc tggccctcgt ctccctaagc
    12121 ttgctgaggg ggaccagggc tatcctttta caaacagttc cccgaacccg ggcaaatcga
    12181 agatatggag tgaatcgtat ggcgagtacc gttcaaggtt tggcttacaa agcatttggt
    12241 ctgcaaatcc acagcgaatt ccctttgccg gagcttcctc ttaccggtga gccggggctt
    12301 attggcagcg tatcagttat tcagggagat ctgaaggaac gctgggaagc tatgccgaag
    12361 atcacgccga gccttggggt atccgggcat gaggttatgt tcgaagctaa ggatacagcc
    12421 ctctttgcta ttcaggacgg caatacgatt acagtatcac ctgctcccgg agcagatccc
    12481 gattgtatcc gtctcttcat tctgggaagc tgcatgggta ttctgctgat gcagaaacag
    12541 attcttccgc ttcatggaag cgcgcttgtt attgaaggca aggcatacgc gctggttggg
    12601 cgttcagggg caggcaaatc aacgctggcc tcgtatttga tggatcaggg gaacccgctg
    12661 atcagtgacg acgttattcc cgttatggtg cacaatggat atccatttgc agttccaggt
    12721 tatccgcagc agaagctgtg gcagcagagc ctggactatt tgggaatgaa ttcgtccctc
    12781 taccgtccgc tgtttgagcg ggaaaccaag tttgccgtac cggtgcatga ccgctttcac
    12841 ggcgaacctg tgcagcttgc agggttgttt gaattatctg ccgccccgga ggctccggtc
    12901 agtctgcagc cgattcaagg catggagcgg ttccacacat tgtacaatca tacctatcaa
    12961 agagcgattg tcgaccggat cgggatccgc gagtggcatt tcggcatgct ggcttctttc
    13021 gtggggcggc tgccgatgta ccggttgacc cggcctgagc agggctttag cgctccgcag
    13081 caaacgtcgc ttattcttga agcattacag cagcaactag tgaaggagat gaactcatga
    13141 atatgaatac ggcggatgtc ttatccctcg aatcagtgct ggtacagcgt gagggaaata
    13201 ttgccagtga tatggacggc gaaaaggtta tgctgaacgt aaaaaatggc aagtactaca
    13261 acctgggtga ggttggcgga gaaatctgga cggcgctggc gtcgcctgta accgctggta
    13321 gggtggtgga gatcatccgg gaaacttttg aggttccgga agagattgcc cgccaggatg
    13381 tgtttgtttt tttgcagagc ctgctggatg aggatcttgc gggtattgta agccagccat
    13441 gattacctta cggcgcatac gcctgctgct gacggtagat aaagccgcct tggcgctcct
    13501 tcccgaggcg ctttggaggc tttttctcgt tcggattcaa ctgttgttcc cgttcgccaa
    13561 aacagctcca cagcttggcc tacaggcgca ggaaacgcct acgctttcca aagagggcga
    13621 tatcgcccgc atccagcatg ttaccaaggc gatccgggtc atgagccgcg ttacaccctg
    13681 gaaaagcact tgcatggtca gagcggtagc gggactcaaa atgctcgaaa aacgcgggat
    13741 tgagagtact ttgtacatgg gggttgccaa ggacaagcag ggccagatga ttgcccatgc
    13801 ctggctgcgc agcgggtcgt attatgtctc gggcgatgat gccatgaagg gctttgtagt
    13861 ggtggagaaa ttcgccaaag tgttacatgc cgattgatcg aggtgggtcc atgcaagcga
    13921 taatgtatta catccggcag cttcagcgct tgtccggagt caaattatat ctgaatctgc
    13981 tcggtatggt gatcagcggc ctgctggaga gctctgctgt tctgctgctg gtgccgatgc
    14041 tgggtatggc tgggattcag cttggcggaa acgcagcagg catcgatctc cctatgttcg
    14101 atttcatatc ggagctgccg cagacctcag cgcttgcgct ggtgcttggt gcgtatgtgc
    14161 tcattgtgct ttgccagaat ctgatctccc gttttgtcgc catccgcaac gtggacattc
    14221 agcagagcta cagccggaag ctgcggtatg aggtgtatgg cgcactgctg cgggccgagt
    14281 ggtctttttt tctgaaaaag cgcacttcgg acctgatcaa tgtaatgacg actgaactcg
    14341 cgagggtgct tgcgggaatc agcagctttc tgcagttctt gacttcgctg ctgttcacgc
    14401 tggtacagat cggcttcgcg ttctggctgt ctccccaaat gactctggct gttctggtct
    14461 gcgcggttct gctgtccctg ttctcccggc gtttcatccg caaagccaag aggctgggca
    14521 gccgcagttc cgagctgggc aagcagtatc tggctggcat tactgatcaa ttgaacggga
    14581 tcaaggacat caaaagcaac aatctggagg attcccggct gagctggatg catgaattca
    14641 ccggcgaagt caaaagcgag cagctggatt atacgcggct tcgcacgaat tcccaactgc
    14701 tgtacaaaat gtcgtcgacc ctgctgattg cggtgttcat cttcgtgtcg ttccgcttcc
    14761 tgcatgtgga ggggccttat ctgttcctgg ttattctggt gttcgcccgt ctgtggccga
    14821 ccttttccaa cctgcagacc ctgctggagc agcttgcttc cgtactgcct gccttcaagc
    14881 agctgcagct gctgcatgag gaatctctgc aggctgcgga gcatggtgcg cacgctcaat
    14941 ccggtactgt ggctgctcct atgacattga agcgtagtct tgaagcacgt gatgtgaatt
    15001 tccgctacaa cccgcaggag gagaattatt cattgcgcag tgtggacgtg gtgatcccgg
    15061 cgaaccgcat gacagccatt gtgggacgtt caggtgcggg aaaaagcaca cttgttgacc
    15121 tgctcatggg attgattcag ccggaatcgg gtgagattct ggcagacgga gagccggtta
    15181 caggggagag gcttctgcct taccggcgct ccatcggcta tgtagcgcag gacccttttc
    15241 tctacaacgc aacgatccgt gacaatctga cgatgattaa gccggatgca agcgaggctg
    15301 agttgtggga ggcgctggag ttcgcctcgt cggctgactt cgtccgcaag ctgccgcaag
    15361 ggctcgatac gctgcttggc gaccgcggcg tccgcctctc cggcggagaa cggcagcgcc
    15421 ttgtgctggc gcgggcgatt atccgcaagc cgtcgatcct ggtgctggac gaagcgacga
    15481 gcgcgctgga taccgagaac gagaagcgga ttcaggaggc gctggagcgc ctgaaagcaa
    15541 cagttacggt cattgtcatt gcgcaccgcc tgtcgaccat ccggggcgcg gaccagatcc
    15601 tggtcatcga ccagggcagg gtcatccagc agggagtgta cagcggcctg gcctccgaac
    15661 aaggcggcat gttcagccgc ctgctcagca gccaggaaga agcgatgtag cctattgcgc
    15721 tgcggagtta ggtgaagttt gctggagcta gcttgaagtg ttgtgagtgc tatgtaagct
    15781 agtagagcat cagactctaa atggtggatt tggagcacaa ctccggcgga acgaatgaag
    15841 gcgtggagag atcacacttc aggtgtaccg attcccagct tgattgatat ctggaccggg
    15901 ggagtctggc tatgtctaac ggactgagga gcacttattt ctattgaaaa tcatttgttc
    15961 cgcaagttaa cggacaccag agcctttaaa gctctttctt gaaaataaag gttttacggt
    16021 ccgtaaggag cagactcgct tgatgttgtt tctcttcacg gaactacaga gggtttggcc
    16081 ttattcacag aaactgcatt ctattgcact tcatgcaatc aaatgaggtc gaatagcctc
    16141 tggtagataa aagcaaagga ttctatcgca caaaacgcaa tagaatcctt ttttgcgtgc
    16201 agagagctga aatctgctgc acgttctgca atagatgcgg cagggggggc cgcctgtctg
    16261 cgtcttcagc aatccgcccg cctgtctgcg tcttcagcaa tccgcccgcc tgctgcgtcc
    16321 cccgcaatcc gcccgcctgc tgcgtccccc cgcaatccgc cctcttgagc gccccgaacg
    16381 gctaagccct ggtagctcca gtcttcgaac ccttgaagcc ccttatttct cctggccgct
    16441 tactttgcgc caggtccaca gaaaggggcg caaagggaaa taaagtacat gcagcggctt
    16501 cggcagcggc agtgtcttgg catctaccgc ataaggatac aggaagccgg ctacatacaa
    16561 gaactggtgc cagcgcgtca gaattgccgg ctgataatat ttgtaatgcc gctcggcctc
    16621 aggctccagg ggaggattat gcagattgat catgcgcgcc acatagaaaa gcgccagctg
    16681 ggcaatccgc cgggccttag gccgcttggt gaagccctcc agggcgggct caaccggcgc
    16741 tgccagcaga tcggcagcca gaagcagtgt ctggccgcca acatcggcgt aattatactg
    16801 ctgtatgagt ccagccagtt tgccggagtc aggcttttgc agcagcagct gacggatatc
    16861 cagcagccat cttagcctcg accagccgtg gcgtgcgcca tgggccgcca gaaaaagaaa
    16921 caagtcttcg ctgcccaaat aatgaacatt ctgcccgaag tggctgctgg tccgcgcaca
    16981 tttccacagc tcgtcgaact ccggttcctt ggaagggccg gggcttaggc gccaatgcac
    17041 ctccacctta atccctttgt ccgggtgatt gaacgtggta tgatgctccc gccatttcca
    17101 gtcgcccagt atactttcga agtcttcctc cttcacataa ccaagacgga ccagcagcgc
    17161 ttcggtttcg gccagttgtg ccatcggcac caggaagtca aggtcacggg aggtccgcag
    17221 cgaaatatcg ccatacagct cctgggcaag caccggtcct ttcaggaaca gggaacgtat
    17281 ttgcagcttt gcaagctctt ccccgagata gcccatttcc ccgctgagct ggagcatctg
    17341 aacggtgttt ttccggtatt cccatttcag gcgctgggta accgcatccg gcacatgctc
    17401 ttcacgcatc cggctaagct taggatatat ataaggaaat acacgatgat gcagggacaa
    17461 ccgtacccat agctcccagt cgatatccgt gaatagttgc ggatttcgtt ctgccgtttc
    17521 atcgatgtca tcagttttga gcacttcaag aagaagcctc agttccttgg aaaagccttt
    17581 tttatccagg gcatacccgt cggtcatagg aaatctcctt acttggattt gtacagcttc
    17641 cttcaaataa ttgttcggtt taaagaacgc actgtgagcg taccatcgaa acccgaaatt
    17701 tgtcaatttc ccggctgatg ccaaatttaa ctttctgaat tcctgccgaa ttcccctaga
    17761 aagtatataa cataacaggc agactattga cgcagtcagc gtggttgata gactaagaag
    17821 agtacttata atactgggat aggcaaaata tctttttaat ggaggaagca atatgcagca
    17881 tctaatagaa tctatcaaat acaacaagga ccgccggttt ccccgcgaag agctggagga
    17941 gatcattagc agaaaagagg aggccattcc ccatttgctg gagattatgc gtgaacttca
    18001 ggcacatccc cagttggcgg aggacccggc gcgattagat tttatgtacg ctgcttatct
    18061 gctttcgcag ttccatgtca cggcattatt ccccattctg gttgagctgt tctcgctccc
    18121 ggaggaatta ttagatatga tcttcagcga cattttgacg gatgccggag gaagaatgct
    18181 ggcatccgtc tacgatggcg accttcattt gttgaagggg ctgatcgaaa acagagaagc
    18241 cggtgaatat gcccgggcgc aggggcttcg ggcgcttacc atacttgtgt atgaaggccg
    18301 gatcttaaga gagacggcag taggctatgt gaaggagctg ctgaccagca aacgggacga
    18361 ttacgattac tatttcaatg cggagatggt gtgcactgca gttgatttat atcccgaaga
    18421 ggtatacgca gaaattgaaa agctgtatga agaggaggca cttgacccct atgtcatcct
    18481 tctggatgag gtgcaggcca ccctgcaaat gagtcaagcc gtagcgatgc agcataaaca
    18541 tgagcaagtg tcttatcaat acattaatga tatccatgcc gagatggaaa agtgggtgtg
    18601 cttccaacct gaaagctcag ggaaaataga attgctaggc gatcagttga aacaaaagtc
    18661 agttaaggtc gacccaaaac ccggacgtaa tgacccatgc ccttgtggaa gcggaaaaaa
    18721 gtataagaaa tgttgcggga aataggagga tgaaatagtc cgctgccccc aggctcgaac
    18781 tcccttttcc aaaacttccc atactatgct aagatagctg caaaatatcc agagacagga
    18841 gtgtttctct gcccatgcct tcgaactgga aaaagaacat tgtgctcttt ctgaccagcc
    18901 agaccatctc tttattcggc tcttcactgg ttcagtatgc gatcctctgg tatatcaccc
    18961 tgaataccca gtccggcctt atgatgacaa tttcgattat ttgcgggttt gtgccgacgt
    19021 tcctgctgtc tccttttgcc ggagtgtggg cggaccggta cagccgcaag ctgctgattg
    19081 tcctctccga cacctgcatc gcggcggcga cgctggttct ggcgctgctg ttcctgatgg
    19141 gctatgacga gatgtggctg ctgtttgtgg tttctgcggt gcgggcactc ggaaccgcag
    19201 tgcaaacacc ggctgtaggt gcaatgctgc cccaacttgt gccggaggag cagctcacga
    19261 aggtaaacgg gacgaacggg agtattcaag ccctgatcat gctgctgtcc ccgatgctga
    19321 gcggcgcgct tctcactgtg gcttcgatcg aggtgatctt ttttatcgac gtgatcacgg
    19381 cggcgctggc ggtggctgcg atgctgcttt ttgtgaaggt gccgctgcat gccaaggctg
    19441 ccgcagaaca gaccctgagc tacttcagcg atatgcggct aggcttcagc tatattaagc
    19501 agcatagcta tatccgtacg ttttttattt tcatggcctt tttccttgta ttggctgccc
    19561 cggttgcttt tctgaccccg ctgcaggtca cacggacctt tggtgatgat atttggcggc
    19621 ttacggcgat cgaaatcgcg ttttccatcg ggatgatgct tggcggtctg atcctggccg
    19681 gatggcaggg attccgcaac agagtgtata cgatgacgat gtcttcactc gcgatgggag
    19741 cattcacgtt tgcgctgggc gttgtccccg tgttctggat ttacttgctc ttcatggggc
    19801 tggttggtct ggcgatgccg ttcttcaata ccccttctac cgtgctgctg caggaaaagg
    19861 tggagccgga ttacctgggc agagtgttcg gcgtgctggg gatgatctca agctccatga
    19921 tgccgctcgg aatgctgatc ttcggaccgc tggccgatgt ggtgaaaatt gaatggatgc
    19981 taatgctcac ggggctgctc atgtttattc agggcttttt cctgctgggc agcaaggtgc
    20041 tggtgaaggc gggcgaacct gcaccagcgg aagcggatgt gaagcaatag aatgcaaggg
    20101 gggggagaat atcattatgg atttcgtgag cggaataccg ggtgcggagt cgtggacaac
    20161 ggtagagccg gtgctgaaag gctggtcgag tgaccggaag ttctacgttg aggatcaggc
    20221 ggggcgcagg ctgctgctga gactctcggc aggccgtacg tttcaacgca aacagcagga
    20281 atatgaagcc attcagcggt tttacaggct gggttttccg atgtcccggg ctgtcgattt
    20341 tggaatatac ggtgagggag aaggcgggga gcaatacgtt tatatgctgc ttacttgggt
    20401 ggatggcgtc cctctggagg agcgtctgcc tggactgccg ccggaggaac agtatcgcct
    20461 gggcgcagag gctggaagaa tattgaagca gatgcatacg atcccggttc aagaggaact
    20521 ccctcattgg gaagcagaca tgcaatctac aatcctctca aggatcaggg aatacgaaaa
    20581 ctgtccctac cgcctggagg acgaccagca agtgatcgcc ttcgtcaggc aaaatgtcgg
    20641 cctgatccat aacgtggaga aagtgcatca tcacggtgac ttccatgtcg gcaatttgat
    20701 ttacacccct gaaggcagga ttggtgtgat cgatttcaac cgctgggata ttggagatta
    20761 tgcggaggaa ttctataagc tgcagatctt cgacagggag cggagcatcc cgtttgcagc
    20821 aggcaagctg gagggatatt tcggcggacc gccgccggag gccttttgga aaagacaggc
    20881 gctgtatgtg gcgtatgcct cgctgtattc catcaagtgg tcgataccct acggcgcagc
    20941 cgacgttcac gatatgatgg accgctaccg tctcgcgctt tcggattatg atcatttccg
    21001 gagaactgtt cccgggtggt acctgggaga cggggagtga tattttgtcc aaaagattga
    21061 aatgaaggag tccagtacgg tagaattgca cttataagcg gcgccttgga atgtttaaat
    21121 cccgggcctg ctatacaacc agcagattta accaagaagg ggattgtaac gtttgaataa
    21181 tgaagaattg aacaacgggc tgccggagaa ttatgaggaa ctgaagaagg ctgccggacg
    21241 ctccgccgat tggagatcgc gtcttgaggc cgtggaggag cttggccgct tcaaccataa
    21301 acaaattatt gatattctga atcgcctcat ggtcagcgat cctgtatata ccgtccaaga
    21361 agctgcatat gagaagctaa aggcctttgg tgaagatgtc acagtgccgt cgaagaacaa
    21421 agccgagctg ttcagaggta tttccaagat tgtgctgcgc atcaagaaga gccttccgca
    21481 ggaccattcg tatgaagagt tcaaggaaaa gctgaagaag atgcggatag acgtctacga
    21541 cgcttacgaa ggcgagaagg gcgagaattt tgacgcctgg ctgcagaaat tgtgggcttc
    21601 ggccacgaaa taaggatgga ccatagagcg gagagacggg gaagattccc tatcgctccg
    21661 cttttttaag cgtaccccaa tccaaggaag caaaggagat cacatgcaaa actcttcagc
    21721 taatgaacca ttaacacgct tatttcagga atatcttgaa ctgtctgccc atttgcgccc
    21781 cgactatacc gccagcttag ggagtggaca cagagagggg cagcctgcga tcagcgacat
    21841 cgtaccggat gcgccaaagc ttttgcaggc catttacagc caggtttccg gtacaagcag
    21901 cgacgaagaa gagcctagtc tgatcgagtt catcccgggc taccggctga ttcatattca
    21961 ggagtatgtg caggaaatca aggtgctgga aagcatcctg aaggaaaagg gctgcagcgg
    22021 cggcgggact gtgctgccca ttctgaccaa ttacggaagt gatttcatct gttattacca
    22081 aagtgaggac ggaacagagc ggatctgcga tctgctgcat gattacggcg acctggccgt
    22141 gatgtatgat tccccggaga agttcctgga gactctgtgt gaattctata agcaggaagt
    22201 ttattttttg gatgaggaag gttatctgga ctgcgatctg gtgctggaag gggaagttgg
    22261 ggcggcactg aatccgtcgg caaactattg gtcggaataa ttggtgaagt gccgcagtgt
    22321 tttttcctgt taatctaaca gacagttcaa aaaggaaggt gaattgcggt gcattacata
    22381 ttcagaaata gaacgcgcca gctgcgggcg ggatgggaga tcctgcttgc agccgcggct
    22441 atttttatac ttaccatgct gatgtccttt ggtttatctc tgcgggtgaa ttccaggcag
    22501 ctgtttgatc cggacggctg ggtacgggtg ttcaaagcaa gcgggctcta catgctggtc
    22561 gttgtttgtt ttacggtgcg tgttgtccgc cggctgccgc tgagcagtat aggcctgtca
    22621 cggccggatg tgaagcggtt cgcacaaggg tttctgagcg gtgccctgtt aatggctatt
    22681 attgttatgg ttctatgggc gctggggaat gcggcctttc agaatgaagg atggaagccc
    22741 cggttcagcc aattggacct gcccggcctg atgcttactg cttgtatagc gggaatctgc
    22801 gaagaagttt tattcagggg atacatacag catttgctgt ccggccgtct gggaactact
    22861 ggggccgtag ctataacctc agtcttgttt tcggcggccc atatggccaa tcccggctat
    22921 aattggatca gtgccctgaa tattgtgctg atagccttga ttttttcgtg tgcaaccctt
    22981 cggacaggga atctgtattt tgcaatggca ctgcaccttt cctggaacct ggttcagggt
    23041 tatgggttcg gtgtctcagt cagcggaaag atcccgcagg gattatactc cgtcctcctg
    23101 ggagggcctg actggattac gggcggacgc ttcggcctgg aggcgagcat cctgacgaca
    23161 atcctgcttg gattaatctg tgctggcttg ctgctggtgc atgtgatgaa gccgcgcaga
    23221 agaacgcagc agacggaatt ttcaagccgt tattagagcg tttctcaaat ataagaaagt
    23281 atatgtacgg gtgccatccg gaaaaaggat ggcacccgtt ttattggagt attctccgta
    23341 tgtctactgc tgctgttact gctgatgtta ctcctgcaca gccttctttt cctgctcccg
    23401 cagttctaca cgccggattt tgccggaggc agttttggga agatctgcga taaattcaat
    23461 tttgcgcggg tatttgtaag gtgcagtcca ctctttgaca tgggactgca gctcttttac
    23521 cagttgaggt gtaccggcgc tgtggtcctt caatacaaca aaggccttaa caaccgagcc
    23581 gcgaatctca tcagggcttg ctaccacggc gcattccttg accagggcat gtttcattaa
    23641 ggcttcctcc acttcaaacg gtccgatggt atagcctgag ctgataatga tatcgtcgcc
    23701 cctgccttcg aaccagaaat agccgtcctc atccttgcgc gcccggtccc cggtgacaaa
    23761 atactcgcca tgggagcagt tcaccttgcg ttcagggtcc ttgtagtaat tctggaacaa
    23821 ggccggcatg ctcaagtgca cggcaatatc tccaacgact cccgcaggca gcgattttcc
    23881 ctccccgtca atcactt
//
