LOCUS       NZ_KN150953            10912 bp    DNA     linear   CON 15-JAN-2024
DEFINITION  Burkholderia pseudomallei strain BEC scaffold2, whole genome shotgun
            sequence.
ACCESSION   NZ_KN150953
VERSION     NZ_KN150953.1
KEYWORDS    WGS; RefSeq.
SOURCE      Burkholderia pseudomallei
  ORGANISM  Burkholderia pseudomallei
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Burkholderia; pseudomallei group.
REFERENCE   1  (bases 1 to 4237627)
  AUTHORS   Daligault,H.E., Davenport,K.W., Minogue,T.D., Bishop-Lilly,K.A.,
            Broomall,S.M., Bruce,D.C., Chain,P.S., Coyne,S.R., Frey,K.G.,
            Gibbons,H.S., Jaissle,J., Koroleva,G.I., Ladner,J.T., Lo,C.C.,
            Munk,C., Palacios,G.F., Redden,C.L., Rosenzweig,C.N., Scholz,M.B.
            and Johnson,S.L.
  TITLE     Whole-genome assemblies of 56 burkholderia species
  JOURNAL   Genome Announc 2 (6), e01106-14 (2014)
   PUBMED   25414490
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237627)
  AUTHORS   Bishop-Lilly,K.A., Broomall,S.M., Chain,P.S., Chertkov,O.,
            Coyne,S.R., Daligault,H.E., Davenport,K.W., Erkkila,T., Frey,K.G.,
            Gibbons,H.S., Gu,W., Jaissle,J., Johnson,S.L., Koroleva,G.I.,
            Ladner,J.T., Lo,C.-C., Minogue,T.D., Munk,C., Palacios,G.F.,
            Redden,C.L., Rosenzweig,C.N., Scholz,M.B., Teshima,H. and Xu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2014) Bioscience, Los Alamos National Laboratory,
            P.O. Box 1663, MS M888, Los Alamos, NM 87545, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2014
            Assembly Method :: Newbler v. 2.6; Velvet v. 1.2.08; AllPaths v.
            39750; phredPhrap v. SPS - 4.24
            Assembly Name                     :: BEC
            Genome Coverage                   :: 279.0x
            Sequencing Technology             :: 454; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/15/2024 02:29:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,952
            CDSs (total)                      :: 6,888
            Genes (coding)                    :: 6,565
            CDSs (with protein)               :: 6,565
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 323
            CDSs (without protein)            :: 323
            Pseudo Genes (ambiguous residues) :: 0 of 323
            Pseudo Genes (frameshifted)       :: 131 of 323
            Pseudo Genes (incomplete)         :: 228 of 323
            Pseudo Genes (internal stop)      :: 25 of 323
            Pseudo Genes (multiple problems)  :: 51 of 323
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:39:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3310201
            Orig. end                         :: 3321113
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KN150953.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10912
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10912
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10912
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5912](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5912
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1315..1452)
                     /locus_tag="DO72_RS29390"
                     /old_locus_tag="DO72_2427"
     CDS             complement(1315..1452)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532784.1"
                     /locus_tag="DO72_RS29390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DO72_2427"
                     /product="hypothetical protein"
                     /protein_id="WP_004532784.1"
                     /transl_table=11
                     /translation="MKRKEKTLPLWAIWLISLVAVIAWCGVHGEPEQPVQQEIRPVSCA
                     "
     gene            complement(1461..1676)
                     /locus_tag="DO72_RS45660"
     CDS             complement(1461..1676)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009971783.1"
                     /locus_tag="DO72_RS45660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_038760828.1"
                     /transl_table=11
                     /translation="MEQSNERGVAERREALFEDLAELGIGAGVCVPTFVVYTPPRTARG
                     DQAAAHAMKELRPLLAKLARRRLILR"
     gene            complement(1680..1901)
                     /locus_tag="DO72_RS29400"
                     /old_locus_tag="DO72_2426"
     CDS             complement(1680..1901)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897093.1"
                     /locus_tag="DO72_RS29400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DO72_2426"
                     /product="hypothetical protein"
                     /protein_id="WP_009897093.1"
                     /transl_table=11
                     /translation="MTDFDIAQAQPRVVAPGVVEVGPFFERYMRGGYFIVKTPSGCREY
                     HWCEQPDASDTTVMMTRDEALQLASHRW"
     misc_feature    complement(1704..1706)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(2217..2999)
                     /locus_tag="DO72_RS29405"
     CDS             complement(2217..2999)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532752.1"
                     /locus_tag="DO72_RS29405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_009909344.1"
                     /transl_table=11
                     /translation="MGGMKTIMNELRDELRVAHIIIRSALSVATFDQKMEWANMNERDA
                     VIGEGITRANERQAAIDGGSVDALYRELKCADRIIANARSLLSDHQFELWTVAIRFAGV
                     MSQNVVRDDVRRRLLTRAVLTCGGNSVADGVAAYQLVAEALVYGGESWIERVRAQRARA
                     REFEDVLMRLRSSNVWKDLVRVLEQFADDAVHHLNAEQRRLLREFITECLKGLDKSFEA
                     GVVSDRVAEKDGDNARNDLAGHEKTGVRELLHVLDSCG"
     misc_feature    complement(3019..3030)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.75"
                     /tool="antismash"
     misc_feature    complement(3129..3142)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.44"
                     /tool="antismash"
     misc_feature    3366..3380
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 20.55"
                     /tool="antismash"
     misc_feature    complement(3367..3382)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.33"
                     /tool="antismash"
     gene            3391..3720
                     /locus_tag="DO72_RS29415"
                     /old_locus_tag="DO72_2424"
     CDS             3391..3720
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909341.1"
                     /locus_tag="DO72_RS29415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DO72_2424"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009909341.1"
                     /transl_table=11
                     /translation="MKTTVDYLDEAKRCLGVESDYALSKRLDIRQSTISGYRAGRSHFD
                     ELTALKIAQACNIDPMEVIAAAAYERAKTPDVRDIWMGAWEKFSKGFRWLALPANACGA
                     LVPQV"
     gene            complement(4087..4515)
                     /locus_tag="DO72_RS29420"
                     /old_locus_tag="DO72_2422"
     CDS             complement(4087..4515)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022362.4"
                     /locus_tag="DO72_RS29420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DO72_2422"
                     /product="DUF2778 domain-containing protein"
                     /protein_id="WP_223872142.1"
                     /transl_table=11
                     /translation="MKRCPECAMSRPYELSDGRYKCRSCGKKFSWTSVWDGRRDRESGG
                     RWGTMRDFVLEHFYGTDRTTWFALYRADGQIDDWTFINGVRRGSFRLHPNGRRGLSEGC
                     VTLVNQQDFVKLRAALKATPMISVPGFAGRAYGTVDVR"
     misc_feature    complement(4553..4567)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 19.89"
                     /tool="antismash"
     misc_feature    complement(4613..4628)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.96"
                     /tool="antismash"
     gene            4656..4967
                     /locus_tag="DO72_RS29425"
                     /old_locus_tag="DO72_2421"
     CDS             4656..4967
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521493.1"
                     /locus_tag="DO72_RS29425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DO72_2421"
                     /product="DUF2282 domain-containing protein"
                     /protein_id="WP_004521493.1"
                     /transl_table=11
                     /translation="MSAKLSFHTALLVGAVASLVASGAHAAPLTKAEADAAVAAHKEKC
                     FGVALKGQNDCAAGPGTTCQGTSTVDFQGNSWKFVQGGTCTSIQVPGGGHGSLTPLKS"
     gene            5001..5912
                     /locus_tag="DO72_RS29430"
                     /old_locus_tag="DO72_2420"
     CDS             5001..5912
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534478.1"
                     /locus_tag="DO72_RS29430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DO72_2420"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_063923570.1"
                     /sec_met_domain="DUF692 (E-value: 5.2e-86, bitscore: 286.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDSVSIAKYMHRDCLHCPASLVGTSFKPCHFADIVDNGLQDGFF
                     EVHAENYMGKGGPPHRALSALRETYPVSVHGVCLSIGGPSPLDAHHLARFRDVVARYEP
                     VLVSEHLAWSSHGGAFYNDLLPLPYTKATLDRVCAHVDQIQEAIARPLLLENPSTYVGF
                     ASSTMSETEFIAAVAGRTGCGLLLDINNAFVSATNHGCSAADYLADFPLEQVGEIHLAG
                     HSEQRDDEDEPLLIDSHDRPVAGPVWALYASVIARTGPVPTLVEWDSQLPAWPVLRAQA
                     LEARRIMDAAVASPVEVADHAL"
     gene            5902..6681
                     /locus_tag="DO72_RS29435"
                     /old_locus_tag="DO72_2419"
     CDS             5902..6681
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521491.1"
                     /locus_tag="DO72_RS29435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DO72_2419"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_004521491.1"
                     /transl_table=11
                     /translation="MRSDAAASDYACAFAPGLTNPSIGAPDDAIAHGHKRVARRYDVYR
                     NNVTVSLIEALAAIYPAVQRIVGVEFFRAMARFHVRETLPVSPLLFEYGRDFPDFIAGY
                     EYARTMPWLADTARIERAWLDAYHAADLSTLTADAFTAIPPDALAAVRLVPHPATRIVR
                     SPYPAVSIFAMNRSDGPVTSLHSAEAEDALVTRPAHDVIVSRLPLGGAVFLISLIDGAS
                     LGDAVAITLEDNPAFDLPASLRGMIEAGVFTAIHSGA"
     gene            6687..7190
                     /locus_tag="DO72_RS29440"
                     /old_locus_tag="DO72_2418"
     CDS             6687..7190
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527026.1"
                     /locus_tag="DO72_RS29440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DO72_2418"
                     /product="DoxX family protein"
                     /protein_id="WP_004521490.1"
                     /transl_table=11
                     /translation="MSDRHTANRAPLRALTSLVSRVRHGIQWLAHPTLAQFALRLALAV
                     PFWKSGILKWNGFLRLNDTAIDLFTSEFQLHLPGGPYPFPAPAVFAFLSGCGEVLFPVL
                     LALGLGTRFAAAGLFVMTCVVELTVPDGWPIHLTWAAMALGIAAWGPGRLSLDYLLGDR
                     APRS"
     gene            7256..7897
                     /locus_tag="DO72_RS29445"
                     /old_locus_tag="DO72_2417"
     CDS             7256..7897
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521489.1"
                     /locus_tag="DO72_RS29445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DO72_2417"
                     /product="DUF1109 domain-containing protein"
                     /protein_id="WP_009973415.1"
                     /transl_table=11
                     /translation="METRDLVTWLATDLTPVDPGAVLQRLYHALLVGFAGSTALLVALY
                     GIRSDMPQLILTTMFWVRFAFPLAVVFSATRLAERLGRPGARLCSVGLATALPVAAMLL
                     ATGGVLIATPPGYRLQLVLGTTWRTTVVDIVLLSLPPLAAALHAMKQLAPTRLVLAGAG
                     AGLLAGAQALVVYSLYCAEMSMPFWGVWYMLSLMLTVALGAMLAPIWLRW"
     gene            complement(7916..8557)
                     /locus_tag="DO72_RS29450"
                     /old_locus_tag="DO72_2416"
     CDS             complement(7916..8557)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009973414.1"
                     /locus_tag="DO72_RS29450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DO72_2416"
                     /product="DUF1109 domain-containing protein"
                     /protein_id="WP_004527028.1"
                     /transl_table=11
                     /translation="MKTNDFIDMLAAGVTPVERGVPARRLGSALAVAAVGAVLLMTIAF
                     GVRPDLSAVMRTPIFWAKLAFPICVAAGAIMATVRLARPGATVGAGRLVVGVGIAAVWI
                     AGLYAVATAAFGDRLAVVLGQTWRVCPFNIVLLSAPGFVAMFVAIRGLAPTRLRTAGAA
                     AGLAASAIATIAYCFHCPEMSPAFWGVWYLLGMALPAALGALVAPIWLRW"
     gene            complement(8554..9099)
                     /locus_tag="DO72_RS29455"
                     /old_locus_tag="DO72_2415"
     CDS             complement(8554..9099)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009973413.1"
                     /locus_tag="DO72_RS29455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DO72_2415"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_038762616.1"
                     /transl_table=11
                     /translation="MHSVESRLRALFVSGLAGHTPDYRSFLSELTRHLRGFLRKRIPQF
                     DADIEDLVQEILLAVHNARHTYRADEPLTAWVHAIARYKLMDFFRTRARREALHDPLDD
                     HTDIFSEPDDDASDAHRDIGRLLDHLPDKQRLSILHVKLQGLSVAEAAQLIGASESVVK
                     VSIHRGLKALAARVRGVV"
     gene            <9560..>9832
                     /locus_tag="DO72_RS40510"
                     /pseudo=""
     CDS             9560..>9832
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492556.1"
                     /locus_tag="DO72_RS40510"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSAVEYARYEDRKVLAAARRPIYAAASEETARQALQDFADGPWGA
                     KYPTIVHSWQRAWEHVVPFYVFPPEIRRVVYTNSIERMNGQLRKII"
     gene            <9833..10287
                     /locus_tag="DO72_RS40515"
                     /pseudo=""
     CDS             <9833..10287
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558218.1"
                     /locus_tag="DO72_RS40515"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="DDE-type integrase/transposase/recombinase"
                     /pseudo=""
                     /transl_table=11
                     /translation="VIDVFARRIVGWRVSSSMSTDFVLDALGQALYARQPGDDGTLIHH
                     PDRGLNTSAFGTANVWPRRVSSRPSAAVATATITRRLKRSTASTRRT"
     gene            <10457..10798
                     /locus_tag="DO72_RS29460"
                     /pseudo=""
     CDS             <10457..10798
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1163: rhodanese domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011326331.1"
                     /locus_tag="DO72_RS29460"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="rhodanese-like domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="FHARDSMEPVSRNELTTRLADGLVTLLDVRPHDEFAQGHLPGALN
                     IPLAALEARLSELPADTEIVAYCRGPYRVLAFEAVTALRARGFKAARLEGGFPEWKAAG
                     LVVETGAAD"
ORIGIN
        1 gtcgcccgtg cttgcgatca atccgccgtc gcactcgaag tcggttccga gtccgccgcc
       61 gctctgaggg cgtgcgctaa ggcaggcggc aacaccttcc cgcgctttgc ggcgcggcgc
      121 cggatacccg agcatgcctt cgcgctcaaa aagaaccgct gcggcacgtc gccagtctcc
      181 aagatgtccg acaacgaaga cacgccgtcg tcgttgaggg acggcgcgag gatgtgattc
      241 cactcggacg tactgagcgt caagaacccg gtatgcgaac ccatacccga gttctgccaa
      301 gcccccgagg aaggttccaa agtcccgtcc gccgttggac gacaggacac cggggacgtt
      361 ttcccagacc agccagcggg gagcgtagcg gcgagcaatc gcaagatagg tgagcatgag
      421 gttgccacgc ggatcatcca gccccttgcg gagtccggcg acgctgaatg actggcaggg
      481 agttccgccg acgagaagat cgatagctgc atcaggccat tccttgaaat gcttcatgtc
      541 cccaagattg ggaaccgttg ggtagtggtg acgcaagaca gcgcagggaa accgctcgat
      601 ttcgctgaac catgctggcc gccagccgag cgaatgccat gcaaccgtag ccgcctctat
      661 gcctgaacaa acggaaccgt agatcattcg cagagcccgg atgcagacgc gcacggggcc
      721 ggtggctcgg cgaaggcggt cgaagcgctc atgcgtctgc cacctcatgc aaatgaatca
      781 agtagtcaac gaccggattc ccggttggcg tctccgtccc cgcacgcgcc agatcaatcg
      841 cgatatgcgc cggaattgac gctctacgcc aatacctata gagcccactt ttcctacccg
      901 ccaaaatctg gcgcatcttc ggcgcgtctt ccatcgtcat cacccgcgaa accacgtgta
      961 gcgcgctgcg gcgaatggcc cgagccattc ggactctccc tccggatcga tgcgcaatgt
     1021 cgatccagcc ggcgagcgat tcggtgcgtc gagcgagcac gccgcgtctt cgctgcggaa
     1081 cgggttgccc tcatcgatcg ccgcccacgt gaacgtctcc cggtcgctgt acggacttgt
     1141 gtttgagatt cggatcatag tcaccccctc acgcgtggac gccgcaacgc atccagcctg
     1201 tgaactcgaa aaaaacgggg cgctttggaa gccgccccgc ccaaaactcg ccgtgctatc
     1261 cgaggccgaa agtttgcacg gcgagtgacc acacgacagc ccggctgccg cgtgctaagc
     1321 gcacgaaacc gggcgaatct cctgctgcac cggctgttcc ggctcaccat gtacaccgca
     1381 ccacgcaatg accgcgacga gcgaaatcaa ccaaatcgcc cagagcggca gcgtcttctc
     1441 cttccttttc atctgctctc ctatcgcaga atcaaccgac gccgcgcaag cttcgcgagc
     1501 agtggtcgca actccttcat cgcgtgagcc gccgcttgat cgccacgcgc cgtacgcggt
     1561 ggggtgtaca caacaaaagt cggcacgcaa acgcccgcgc ctatcccaag ttccgcgagg
     1621 tcttcgaaca gcgcttctcg ccgttcagcg accccgcgct cattcgattg ctccatgccc
     1681 taccaccgat gcgaagcaag ctgtaacgct tcatcgcgcg tcatcatgac cgtcgtgtcg
     1741 ctcgcgtccg gctgctcgca ccagtgatat tcccggcaac ccgacggcgt tttcacgatg
     1801 aagtaaccgc cgcgcatata ccgctcaaag aaagggccga cttcgaccac ccccggcgca
     1861 acgacgcgcg gctgtgcctg cgcaatatcg aaatcagtca cgcgagcctc acaatcgaat
     1921 acacccgccc gatgcgcttc aacgtcgcgc gtggtcgatc gacatcagcc gcatcgctcg
     1981 cgcgacggcg tgccatctgc tccgcgttgc tcgcgcgaat cattcgccga atcgcaatcg
     2041 cttccgcctc ttgttgatta ggattgctat aaatatgagc cattgaatca ccccgctatg
     2101 tgtgtttgtg ctgtcaacag ttggggtcgc tgcggcgggc tatacttact cgtccctcaa
     2161 gacccatcca atcgcgataa acagaggcag aaatgaacac gaaggattct ccgaattcac
     2221 ccgcaactat cgagaacgtg cagcaattca cgaacgccgg tcttttcgtg accggccaga
     2281 tcattgcgcg cgttatcccc gtctttctca gccaccctgt cactgaccac gccagcctca
     2341 aacgacttat cgaggccctt aaggcactcg gtaatgaact cgcgaaggag tcgccgctgt
     2401 tcagcgttca agtgatggac ggcatcatcg gcgaattgct cgagaacccg cacaaggtct
     2461 ttccacacgt tgcttgatcg caggcgcatc aacacatctt cgaactcgcg agcccgcgcc
     2521 cgctgtgcac ggactcgctc gatccaagac tcgccgccat acacgagcgc ctcggcgact
     2581 agctgatagg cagcgacacc gtccgcgacc gaattaccgc cacacgtgag tacggcacgg
     2641 gtcagcaacc gccgtcgaac atcgtcgcgc acaacgttct gcgacatgac accggcaaac
     2701 cggatcgcca cggtccacag ttcaaactgg tgatctgaaa gcagcgatcg agcgtttgcg
     2761 atgatgcgat ccgcgcactt caactcgcga tacaacgcat cgacgctgcc accgtcaatc
     2821 gccgcttgcc gttcgttggc acgcgtgatg ccctctccga tcacggcgtc acgctcgttc
     2881 atgttcgccc attccatctt ctggtcgaac gtggcaactg aaagtgcgct gcggatgatg
     2941 atgtgcgcga cgcgcaactc gtcgcgcagt tcgttcatga tcgtcttcat gccgcccatc
     3001 ccccgcggaa cgcgaccggc gttcgcgtcc acttttggac taatccgatc attgcgtcca
     3061 taaacgcacg gaaatacggc tcgatcgtct ggcgcaccag cgccgaaggt gtaagcgttt
     3121 gttgcagcga aacgtgcatt tgacttcccc tttctcaacc ccttgaacgg tgtacggctt
     3181 gaaagtcgac cggcggctgg atagctagtc cagcaccggc ggggttggga accggctgcc
     3241 gtgtcgacag gcggataata cttcgcaccc tcgaagcatg caagcctcga agcattgtga
     3301 tcacgaagca ttgaggtcat gaacgttgca tgttcgagaa catcgaagta atcttgccgt
     3361 cgggttgata ttgattttta aggggtttca atgaagacga ccgtcgatta cctcgacgaa
     3421 gccaagcgtt gccttggtgt cgagtccgac tatgccctgt ctaagcgact ggacattcgc
     3481 caatccacca tcagcggcta tcgtgccggc cgcagccatt tcgacgagct gaccgcgctc
     3541 aagatcgcgc aggcgtgcaa tatcgatccg atggaagtga tcgcagcggc cgcttatgag
     3601 cgcgcaaaaa cgcctgacgt tcgcgatatc tggatggggg cttgggaaaa attttccaag
     3661 ggttttcggt ggctggcgct acccgctaac gcttgcgggg ccttggtccc gcaggtgtaa
     3721 cgccagctaa agttagcgat cccttttgat attatgtcaa atattgcacg tagactgatc
     3781 gcgacgattc gagcaatcaa agcagccctg tccctgacaa tcacgcgtcc ggcaacagcg
     3841 tagcaagccc aaaatcatct ccttcgacac gcctgccatg agcaggcgtt ttttatgctc
     3901 ggcaatctcg taatatacgc gccgtcgctt cgcgtcatgg attccgcgcc gagtattgcg
     3961 gatcacccag tagagattcc tatatcccga gccacgtcgg ccagttcttg cgccgacagg
     4021 ccgccttggc gagcgccaac gtaaccgcca gcgtcgtcac cacgttaaac acgattcgag
     4081 acgttttcat cgaacatcga ccgtaccata tgcgcgaccg gcgaaaccgg gcacgctgat
     4141 catgggcgtt gccttgaggg cggctcgcag ctttacgaag tcctgttgat tcaccagagt
     4201 gacgcagcct tcactcaagc ccctccgccc gttcggatga aggcggaagc tccctcgcct
     4261 cacgccattg atgaaggtcc aatcgtcaat ttggccgtcc gcacgataca gcgcaaacca
     4321 agtcgtccga tcggttccgt agaagtgttc caaaacgaaa tcgcgcatgg tgccccaacg
     4381 cccgcctgac tctcgatctc gccgcccatc ccagaccgac gtccaactga atttcttgcc
     4441 acagctacga cacttgtacc gtccatcgga cagttcatag ggcctgctca tcgcgcattc
     4501 gggacagcgt ttcatcgtcg gaccagcata acgctggtcc gatctgggtg ggaattacca
     4561 aaaaatttcg accgctgtaa ccatttgcgc cgatcgcacg aattaacttg cgtacccaca
     4621 acgggtacct tccacatcaa tcatcggaga acaccatgtc ggcaaaactt tcgttccata
     4681 ccgccctgct cgtcggcgcc gtcgccagcc tcgtcgcctc gggtgcgcat gccgcgccgc
     4741 tcacgaaagc cgaggccgac gccgccgtcg ccgcccacaa ggaaaaatgt tttggcgtcg
     4801 cgttgaaggg gcaaaacgac tgcgcggcgg gccccggcac cacttgccag ggcacctcga
     4861 ccgtcgactt ccagggcaac tcgtggaagt tcgttcaggg cggtacgtgc acgagcatcc
     4921 aggtgccggg tggcggtcac ggctcgctca cgccgctgaa gtcgtaaagc gcatcgacaa
     4981 gaggaaatcc ggaggcgctc atgagtgatt ctgtcagtat tgcgaaatat atgcaccgtg
     5041 actgcctcca ttgcccagca tcgctggtcg gcacaagctt caaaccttgt cactttgcgg
     5101 acatcgtcga taacggcttg caggacggct tcttcgaggt gcacgcggaa aactacatgg
     5161 gcaagggcgg tccgccgcat cgagcgctaa gcgcgctgcg cgaaacctat ccggtttccg
     5221 tgcacggcgt gtgcctgtcg atcggtgggc catcgccgct cgatgcgcat cacctcgcac
     5281 gttttcgtga tgtcgtcgcg cgctacgaac ccgtgctcgt ctccgagcat cttgcgtggt
     5341 catcgcacgg cggcgccttc tacaatgatc tgctgccctt gccctatacg aaagcgacgc
     5401 ttgatcgggt ttgcgcgcac gtcgaccaga ttcaggaggc gatcgcgcgt ccgctgctgc
     5461 tcgagaatcc ctcgacctat gtcgggttcg cgtcgtcgac gatgagcgaa accgaattta
     5521 ttgcggcggt cgccgggcgt accggttgcg gcctgttgct cgacatcaat aacgcgttcg
     5581 tgtcggcgac caatcatggc tgctcggccg ccgactatct cgcagatttt ccgctcgaac
     5641 aggtcggcga aatccatctg gccggacaca gcgaacaacg cgacgacgag gacgaaccgc
     5701 ttctcatcga cagccacgat cgcccggttg ccggccccgt atgggcgttg tacgcgagcg
     5761 tgattgcccg cacagggccg gtgccgacac tcgtcgaatg ggacagccag cttcccgcct
     5821 ggcccgtgct gcgtgcgcag gcgctcgaag cccgccggat catggacgct gccgtcgcat
     5881 cgcccgtgga ggtagccgat catgcgctct gatgctgccg cttccgatta cgcgtgcgcg
     5941 ttcgcgcctg gcctcacgaa tccgtcgatc ggcgcgccgg acgacgcgat cgctcatggg
     6001 cacaagcgcg tcgcccgccg ctacgacgtc taccgcaaca acgtgacggt cagcctgatc
     6061 gaagcgctcg cggcgattta cccggcggta cagcgcatcg tcggtgtcga gttcttccgc
     6121 gcgatggcgc gcttccatgt acgcgaaacg ctgcccgtct cgccgctgct gttcgaatac
     6181 gggcgcgact ttccggactt catcgccggt tacgaatacg cgcgcacgat gccgtggctc
     6241 gcggataccg cccgtatcga gcgcgcatgg ctcgacgcgt atcacgcggc cgatctgtcg
     6301 acgctgaccg ccgatgcgtt cacggcgatc ccgccggatg cactggccgc ggtgcgtctc
     6361 gtcccgcatc cggccacccg tatcgtgcgc tcgccgtatc ctgccgtttc gatctttgcg
     6421 atgaaccggt ccgatggccc cgtcacgtcg ctgcactccg ccgaagccga ggatgcgctc
     6481 gtcacgcgcc cggcgcacga cgtgatcgtc tcccggctgc cgctcggtgg cgcggtgttc
     6541 ctgatcagcc tgatcgacgg tgcctcgcta ggcgacgcag tcgcgatcac cctcgaagac
     6601 aatcccgcct tcgatctccc ggccagcttg cgcggcatga tcgaagccgg cgtgtttacc
     6661 gccatccatt ccggagcctg accgccatgt ccgatcgaca caccgccaat cgcgcgccgc
     6721 tgcgcgcgct cacctcgctt gtcagccgcg tgcggcatgg tatccagtgg ctcgcccacc
     6781 cgacgcttgc gcagttcgca ttgcgactcg cgcttgcggt gccgttctgg aaatctggca
     6841 ttctcaagtg gaacggcttc ctgcggctca acgacactgc gatcgatttg ttcacgagcg
     6901 aattccagtt gcatctgccc ggcggcccat acccgttccc cgcaccggcc gtgttcgcct
     6961 ttctgtccgg ctgcggggag gtgctgttcc cggtactgct ggcgctcggc ctcggcacgc
     7021 gattcgcggc ggccggcctg ttcgtgatga cctgtgttgt cgagctgacg gtgccggacg
     7081 gctggccgat ccacctgacg tgggcggcaa tggccctcgg tatcgcggcg tggggcccgg
     7141 gccgcctctc gctcgactat ctgctcggtg atcgcgcgcc gcgcagttga gcccgtggcg
     7201 cggcaacacg ccgccgtgcc ggtccgcaac gctgcccgtc ctgcgcatct tcgcaatgga
     7261 aacccgagac ttggtcacct ggctggcaac cgacctgacg ccggttgacc ccggcgcagt
     7321 tttgcagcgg ctctaccacg cgctcctcgt cggcttcgcc ggcagcaccg ccctcctcgt
     7381 cgcgttgtat ggcatccgca gcgatatgcc ccaattgatc ctgacgacga tgttctgggt
     7441 gcggttcgcg ttcccactcg ccgtcgtctt ctcggcaacg cggctcgccg agcgccttgg
     7501 ccgccctggc gcgcgactgt gttccgttgg gcttgcgacc gcgctgccgg ttgccgcgat
     7561 gctgcttgcc acgggcggcg tgttgattgc gactccgccc ggctaccggc tgcaactggt
     7621 gctcggaacg acgtggcgga ccacggtcgt ggacatcgtg ctgctgtcgc tgccgccgct
     7681 cgccgccgcg ttgcatgcga tgaagcaact cgcgccaacg cgtctcgtgc tcgccggcgc
     7741 gggtgccggc cttcttgccg gcgcacaggc gctcgtcgtc tattcgctct actgcgccga
     7801 gatgtcgatg ccgttctggg gtgtctggta catgttgtcg ctcatgctaa cggtggccct
     7861 cggtgcgatg cttgccccga tctggctgcg ctggtaggca cggcgcccca gcccctcacc
     7921 agcgcagcca gatcggagcg acgagtgcgc cgagtgcggc cggcaacgcc atcccgagca
     7981 gataccagac gccccagaat gccgggctca tttccgggca gtggaagcag taggcgatcg
     8041 tcgcgatcgc actcgcggcg agccccgccg ccgcgcccgc ggtacgcaac cgcgtgggcg
     8101 ccaacccccg gattgcgacg aacatcgcaa cgaaaccggg cgccgacagc agaacgatgt
     8161 tgaacggaca cacgcgccac gtctgaccga gcacgaccgc gagccggtcg ccgaatgcgg
     8221 ccgtcgcgac cgcataaagg cccgcgatcc agaccgccgc gatgccgacg ccgaccacca
     8281 gccggcccgc accgacggtc gcgccggggc gggcgagccg tacggtcgcc atgatcgcgc
     8341 ctgccgccac gcaaatcgga aacgcgagct tcgcccagaa gatcggcgtg cgcatcacgg
     8401 ccgacagatc ggggcgcacg ccgaacgcaa tcgtcatcag cagcacggcg cccaccgcag
     8461 cgacggcaag cgcactgccc aggcgcctgg ccgggacgcc gcgctcgacg ggtgtgacgc
     8521 ccgccgccag catgtcgatg aaatcgttcg ttttcatacc actcctcgca cgcgcgccgc
     8581 gagcgccttc agtcccctgt gaatgctgac cttgacgacg gattcggacg caccgatcag
     8641 ttgcgccgcc tcggcaaccg acaagccctg cagcttcaca tgcaggatcg atagccgttg
     8701 cttgtccggc agatgatcca gcagcctgcc gatgtcgcga tgcgcgtcgg acgcgtcgtc
     8761 gtcgggttcg gaaaagatgt ccgtgtgatc gtcaagcgga tcgtgcagcg cttcgcggcg
     8821 ggcgcgcgtc cggaagaaat ccatcagctt gtagcgcgcg atggcatgta cccacgccgt
     8881 cagcggttcg tccgcgcgat acgtatgacg tgcattatgg acggcgagca ggatctcctg
     8941 cacgagatct tcgatgtccg cgtcaaactg cgggatccgc ttgcggagaa agccgcgcaa
     9001 gtggcgggtc agctccgaaa ggaacgaccg gtaatccggc gtatggccgg cgaggccgct
     9061 gacgaacagc gctctcaagc gggattccac ggaatgcaag atcgggcccc ggacctagcg
     9121 caccaggcca tcttgccgcg gctgcgtccc gccccgaacg ccggggctca cgcgtcgcgc
     9181 tgccagcgcc agcgagtgcg gccgacccgt gagcccgcga gcggctccgt ttgattcatc
     9241 cgtgatcgct actcgcccgt acccgatgtc catgctgcgg ttccttgctc aatccgatgt
     9301 ttcgcctggg ccgatgcatc ggccgagcct gccgtcacga taccgtgcgg cgggcaatcc
     9361 tgatcccccc acgcctggat acggtcgatc gcgatctcgg gcgccgggtg ctccgctaac
     9421 atgcctccat gtctcggcaa gcaactttag atcaagcaat ctagaatatc gtggtagcca
     9481 actcattgca atgcggcgcg ttgtgaggtc ggacggaatg cgtggaggcc aacgggttaa
     9541 gcagcgatcc gattcagata gaagcgctgt cgaatacgcc cgctacgagg accgtaaggt
     9601 gctcgccgca gcgcggcgtc cgatctacgc ggctgccagc gaagagacgg cacggcaggc
     9661 gctgcaagac ttcgccgacg gaccttgggg cgcgaagtat ccgacgatcg tgcattcgtg
     9721 gcaacgcgcg tgggagcacg tcgtgccgtt ctacgtattt ccgccggaga ttcggcgggt
     9781 cgtttatacg aactcgatag agcgcatgaa tggccaactg cgcaagatca tcgtgatcga
     9841 cgtgtttgcc cgtcgcatcg tcggttggcg cgtcagttcg tcgatgagca cggactttgt
     9901 tctggatgcg cttggacagg cgctgtacgc gcgccaaccg ggcgacgatg ggactttgat
     9961 acaccacccc gatagggggc tcaatacgtc agcattcggt acagcgaacg tttggccgag
    10021 gcgggtatcg agccgtccgt cggccgccgt ggcgacagct acgataacgc gccggctgaa
    10081 acgatcaacg gcctctacaa gacggacctg attcatcgac gcgccccgtg gaaaacgagg
    10141 caatccgttg aactggcgcc cctggaatgg gtggcccggt acaaccatcg gctgatggaa
    10201 ccgctcggcc atatcccgcc tgccgacgct gaggcaaact actacaggca actcggaaat
    10261 gctgctgaag tgcctgcatt aacttaaacc aaccggcctc cacgattccc ggcgcggttc
    10321 acttcgactg cggcgaaggc ttcatcggcg cgtttcttcg catcggccgc ggcgcccgtc
    10381 gcgctcgtga cggccgcgct cccgcttcgc gcacgtcggc gaagcgctcg tcgtcgttct
    10441 cgcagttgcg agcgatttcc atgcgcgcga ttctatggag ccggtatcgc gaaatgagct
    10501 gaccacgcgt cttgcggacg gtctggtcac cttgctggac gtgcgcccac atgacgagtt
    10561 cgcacaaggg catctgccgg gcgcgctcaa tatcccgctg gccgcgctgg aggcccgcct
    10621 gagcgagctc ccggccgaca cggaaatcgt ggcgtattgc cgcggcccgt accgtgtact
    10681 tgcattcgag gccgtgactg ccctgcgtgc cagaggcttc aaggccgctc gtctcgaggg
    10741 cggctttcct gaatggaaag cggccggtct cgtcgtcgag accggcgccg ccgactgacg
    10801 tgagccgaag cgacgcacgg catcgcactc ctgtcgatca acctcgcgga tcagcggccg
    10861 gtttcatgcg ctgccagcgt gcgatcgagc acgacgagcg tccggtacag ca
//
