LOCUS       NZ_KN150941            10852 bp    DNA     linear   CON 15-JAN-2024
DEFINITION  Burkholderia pseudomallei strain BFD scaffold1, whole genome shotgun
            sequence.
ACCESSION   NZ_KN150941
VERSION     NZ_KN150941.1
KEYWORDS    WGS; RefSeq.
SOURCE      Burkholderia pseudomallei
  ORGANISM  Burkholderia pseudomallei
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Burkholderia; pseudomallei group.
REFERENCE   1  (bases 1 to 3267697)
  AUTHORS   Daligault,H.E., Davenport,K.W., Minogue,T.D., Bishop-Lilly,K.A.,
            Broomall,S.M., Bruce,D.C., Chain,P.S., Coyne,S.R., Frey,K.G.,
            Gibbons,H.S., Jaissle,J., Koroleva,G.I., Ladner,J.T., Lo,C.C.,
            Munk,C., Palacios,G.F., Redden,C.L., Rosenzweig,C.N., Scholz,M.B.
            and Johnson,S.L.
  TITLE     Whole-genome assemblies of 56 burkholderia species
  JOURNAL   Genome Announc 2 (6), e01106-14 (2014)
   PUBMED   25414490
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267697)
  AUTHORS   Bishop-Lilly,K.A., Broomall,S.M., Chain,P.S., Chertkov,O.,
            Coyne,S.R., Daligault,H.E., Davenport,K.W., Erkkila,T., Frey,K.G.,
            Gibbons,H.S., Gu,W., Jaissle,J., Johnson,S.L., Koroleva,G.I.,
            Ladner,J.T., Lo,C.-C., Minogue,T.D., Munk,C., Palacios,G.F.,
            Redden,C.L., Rosenzweig,C.N., Scholz,M.B., Teshima,H. and Xu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2014) Bioscience, Los Alamos National Laboratory,
            P.O. Box 1663, MS M888, Los Alamos, NM 87545, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2013
            Assembly Method :: Newbler v. 2.6; Velvet v. 1.2.08; AllPaths v.
            44837; phredPhrap v. SPS - 4.24
            Assembly Name                     :: BFD
            Genome Coverage                   :: 304.0x
            Sequencing Technology             :: 454; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/15/2024 02:34:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,650
            CDSs (total)                      :: 6,571
            Genes (coding)                    :: 6,399
            CDSs (with protein)               :: 6,399
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 87 of 172
            Pseudo Genes (incomplete)         :: 122 of 172
            Pseudo Genes (internal stop)      :: 22 of 172
            Pseudo Genes (multiple problems)  :: 48 of 172
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:41:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2282051
            Orig. end                         :: 2292903
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KN150941.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10852
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="14"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10852
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10852
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5852](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5852)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            448..1068
                     /locus_tag="DP56_RS09325"
                     /old_locus_tag="DP56_2649"
     CDS             448..1068
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894570.1"
                     /locus_tag="DP56_RS09325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DP56_2649"
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_004194492.1"
                     /transl_table=11
                     /translation="MNWIAFSHGAMLCASLIVTIGAQNAFILRQGIMRSHVGKIVLLCT
                     LSDMLLIGAGVGGASVLVERYPAFVHAVLYVGLAYLAWFGLSALRRAFKPGHETLDATG
                     AVAAPPAQRAWPIVLMTLAFTWLNPHVYLDTFLLIGTAGAREPEGARGAFAIGAMAVSA
                     VWFVALGYGARLLAPWFKRALAWRVLDGAIGGMVLFLAAVQLR"
     gene            complement(1240..2229)
                     /locus_tag="DP56_RS09330"
                     /old_locus_tag="DP56_2650"
     CDS             complement(1240..2229)
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852411.1"
                     /locus_tag="DP56_RS09330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DP56_2650"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_004545057.1"
                     /transl_table=11
                     /translation="MARRKLERWSALDLAEGLQLAHAVAALQTLGVVDAMAEPTTAQTL
                     SAACDLDPELLRGVLEYAASRTNLIRKTGDRFAASEHYTAQARFLLNLYAGAYADNASR
                     LATLLRRPALAGTTVDLVAHARAFEASEAGGGALAAIISQLHLNHVLDLGCGAGTLLHA
                     LAADDREFVGWGLDRNPSMCKAARMRARQAGIAARVKVFQGDGRHPGASIPPRVLARVR
                     NVVASQFVNEMFRGGTSRAQAWLRRMRRLLPDRLLVIADYYGRLGHGFQTPHRETLLHD
                     YAQLISGQGVPPPNADAWLAMYRAAGCRPLHVIEDRAATTRFVHLVAL"
     gene            complement(2243..4186)
                     /locus_tag="DP56_RS09335"
                     /old_locus_tag="DP56_2651"
     CDS             complement(2243..4186)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004549365.1"
                     /locus_tag="DP56_RS09335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DP56_2651"
                     /product="radical SAM protein"
                     /protein_id="WP_004544988.1"
                     /sec_met_domain="PF04055 (E-value: 7.7e-14, bitscore: 49.8,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLLLVDNLVMPEEGSLAYLDVHPHLGLLALAAVAEADGHRVRIY
                     DPKRLIRDGTLAYDATLYERASRAILAERPDAVGFTALGCSFLFALNVAALLKRREPDL
                     PVLLGGPHATMLHRQILERFPQFDIIVRYEADEILPAVLDCLEQRTFDVIPGLSWRATG
                     RGAPLRFTDGKPKVEELDRLPIASYDHYPVDDLGLSMLRIEAGRGCPFACTFCSTAGFF
                     QRSFRLKSAERLVHELDILHRRYRVSDFKLDHDMFTVNRHKVMAFCEAVAGRGYRWRAS
                     ARIDCVDEALLRKMADAGCVNLYFGVETGSERMQTICRKRLDLRLVEPILAAADSFGIE
                     TTASFITGYPQESGQDQDDTLDMIGRCARRPSCLTQLHMLAPEPGTPLFDEHGAQIAYD
                     GYGGPYNTRLLGPEDEREVLDHPDIFQTYYYYPAALPRARHIFAVEAVDVLRRAGPIIL
                     AYALREFGGRLSRLISAWRRFADETSPGALPDAAGLEAFVAARFGRGHHLHSLFRYALR
                     LSAARAPADVVAEGPYEPDRPCMLAENVGLLEDIHNCEALVDRLRRSNDGAPALGDADV
                     GGLGAYIVRADGEAVTGYWVESGIAAILDLFRAPRSCRAAAQWLAHVTRNDGVDASIFE
                     PLLRRGILVPAR"
     gene            complement(4183..5004)
                     /locus_tag="DP56_RS09340"
                     /old_locus_tag="DP56_2652"
     CDS             complement(4183..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543057.1"
                     /locus_tag="DP56_RS09340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DP56_2652"
                     /product="hypothetical protein"
                     /protein_id="WP_009932366.1"
                     /transl_table=11
                     /translation="MNARTIHALIAASTKDPQLIERWRRHPDLLRGAGIAPGDLDWRAL
                     EHFVGLSLKVRHNGLRGDFPLSFRLLSVVGLEIEVFAAYASDCAESGHVFAATPQERAR
                     DLIAFLDRWLDPAQRNHVLLWDILRHEYALACLRQAGDAPLPDARHRARATAGGIPTLN
                     GSVVQHVMRCNPDDLAAALHQKEPPLDAVPAVDTYCCYWRSAETGEIRLLRVDTLGYCA
                     IEAIDGRRSAADICHRLLGNRRLLPQFLSSLDQLAGIGIVRFARPLGCAAA"
     gene            complement(5001..5852)
                     /locus_tag="DP56_RS09345"
                     /old_locus_tag="DP56_2653"
     CDS             complement(5001..5852)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603954.1"
                     /locus_tag="DP56_RS09345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DP56_2653"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_038756459.1"
                     /sec_met_domain="DUF692 (E-value: 3e-34, bitscore: 115.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAFDRDAGAPRIGLAYGPGMAEFAARHAHLVDYIEVPFELLRFS
                     PTIAELQQTIPFVLHCASLSVAGFVPPDDSTVDAIERTAVRTGTPWIGEHLAYISADPI
                     GEALGGTHEPTSLSYTLCPQLSDETVRRVTDNLAVLAPRFPVPLIVENSPQYFSIPGST
                     MGMTDFIRAIADRCDVGLLLDLSHFLITAYNTGADVHREFARLPLERVVEVHLSGMSIQ
                     SGAAWDDHSLPASPILFELLERLLAVARPRALTFEYNWSPYFPLSVLTSHIERARALMG
                     LA"
     gene            complement(6026..6226)
                     /locus_tag="DP56_RS09350"
                     /old_locus_tag="DP56_2654"
     CDS             complement(6026..6226)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525608.1"
                     /locus_tag="DP56_RS09350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DP56_2654"
                     /product="hypothetical protein"
                     /protein_id="WP_004201048.1"
                     /transl_table=11
                     /translation="MATKPKKTKGVSVSIEGKLPKMTLDMPVDAKKIKAIQKCLENGKL
                     TITMSKVDLAGGRMGEGYLYD"
     misc_feature    6203..6212
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.0"
                     /tool="antismash"
     misc_feature    complement(6307..6321)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.99"
                     /tool="antismash"
     gene            complement(6376..7782)
                     /locus_tag="DP56_RS09355"
                     /old_locus_tag="DP56_2655"
     CDS             complement(6376..7782)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009981062.1"
                     /locus_tag="DP56_RS09355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DP56_2655"
                     /product="cytochrome P450"
                     /protein_id="WP_038756461.1"
                     /sec_met_domain="p450 (E-value: 8.6e-35, bitscore: 117.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGLPLSLPRVGSTDSLFADPLAFLARARAQCGDVFVLREHGPIF
                     SRASDCSGVIAVFGEHRLRQVLTETDTFALPMSAAAKMALPKNLVNLNRGLHSMREPEH
                     GRHKRLLTGTANRALFDAHRSGMQTDLSRFCEMLNGDGRISVVSRMRELTAKLASRLFL
                     GPQCEEDAELTFLLSAYFTLRREASSPNAHDPLQYRDALIGAGRQLDRALRARIRRYRK
                     APAGECAGLLQRLATAGQPGSPALSEDEIVGHANVMFVSSTEPVAISLAWLLLVLSQLP
                     DLRRALRAESAARASSPASPYDAPLLERVINETLRLLTPNALMVRATTQAVSLQGVALP
                     ARCEIVVCPFLVHREANAFARPHAFLPSRWETARPSPYEYFPFGAGGHFCAGRNLALSL
                     IGEVLSTLLSRFDFVLDIEQFIDWRIHIMLMPKGDPTLMAHPVDERRDTPSPKWRGPVA
                     ELFHFAPGLS"
     misc_feature    complement(6559..6561)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            8190..8897
                     /locus_tag="DP56_RS09360"
                     /old_locus_tag="DP56_2656"
     CDS             8190..8897
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548999.1"
                     /locus_tag="DP56_RS09360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DP56_2656"
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_004196523.1"
                     /transl_table=11
                     /translation="MRHAHRFSRRECGMSDSFGHSPFEMFRQMFGGHRRGGGRHGFGPF
                     GGFGGGGPGGFGGFGDDAMPRGRQFSADDLQLLLLALLAEQPSHGYELIKALDTRSSGF
                     YAPSPGMVYPALTYLEELGYVTVQAEGNRKRYALADAGRAHLDAQRERVEMLFAKLAHL
                     GRKMEFMRRAFAGEAPAAPEDDTRGWLPEFVEARMALKQALFRRSGANADEQRRIAAIL
                     RRAVADIEGRAQG"
     gene            8937..9764
                     /locus_tag="DP56_RS09365"
                     /old_locus_tag="DP56_2657"
     CDS             8937..9764
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /GO_process="GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1200: vibriobactin
                     utilization protein ViuB"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532447.1"
                     /locus_tag="DP56_RS09365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DP56_2657"
                     /product="siderophore-interacting protein"
                     /protein_id="WP_004194445.1"
                     /transl_table=11
                     /translation="MTQPTTERAVVRVRHTLKFRLLQVKRVVDVTPHLKRVTLAGDELD
                     DFESASFDDHVKVFFPPPGEDAPARPEFGPNGIVFPQDRPRPVARDFTPRRYDRAAREL
                     DLDFVLHHPGPASQWAAQARVGQTLGIGGPRGSFVIPKDFDWHLLIGDDTALPAVARRL
                     EELPAGTRVAAVLEVADPSAQIAFETDANLYVVWRHRGDAASGERGASRLVDALRELPL
                     PAGEGYVWAAGEAAAMRGVREHLCRERGVAKSRIRASAYWKHGAIAVHESLED"
     gene            complement(10088..10336)
                     /locus_tag="DP56_RS42845"
     CDS             complement(10088..10336)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194583.1"
                     /locus_tag="DP56_RS42845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_011852397.1"
                     /transl_table=11
                     /translation="MWRRADARASRDASGRWNLNARAGRRRCGVDRRENGGEANETALS
                     ARGMAAKACDAMGQRGAAALERRGGAVRSGANQSRLP"
     gene            10530..10766
                     /locus_tag="DP56_RS09370"
                     /old_locus_tag="DP56_2658"
     CDS             10530..10766
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196519.1"
                     /locus_tag="DP56_RS09370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DP56_2658"
                     /product="hypothetical protein"
                     /protein_id="WP_004196519.1"
                     /transl_table=11
                     /translation="MTRHHTHAGIPRTLKIVGYCGLAAIALALFVAMCAILKDSALDRE
                     AARQLQNGNVELHGGADVEPGSTNDAAQARTPG"
ORIGIN
        1 caaccgcgtc gacgaaccag gtcgcgacgc tgtcgtcgtt gacggccacg cgcagcgtcg
       61 gccacgcccc cgcttgcgcg cccgcgaacg cgggcatgtg cccggacagc tcggcctcca
      121 gcatccgcac gcgctcggtg tggcggcaca gcagcgcgcc cgaggtcgtc gcgacgcacg
      181 gctgcccgcg cttcacgagc acgctgccca cgcgctcctc caagagcttc acgcgctgcg
      241 acaccgccga aggcgacacg ttgagcgctt tcgccgcccg ctcgaacgag ccgtggcgga
      301 tcacttcggc aagcgcatcg agcagtgcat agtcgagcat cagttttcct taatcagcat
      361 tagatcattt agcttttctt actcaagcga tcatcgcaga ctagcgcctc ttgccccgtt
      421 agtccaccgc cggaagccgc aacgaacatg aactggatcg ctttttctca cggcgcgatg
      481 ctctgcgcat cgctcatcgt caccatcggc gcgcagaatg cgttcatcct ccggcagggc
      541 atcatgcgtt cgcacgtcgg caagatcgtc ctgctctgca cgctgtcgga catgctgctg
      601 atcggcgcgg gcgtcggcgg cgcatcggtg ctcgtcgagc gctatccggc cttcgttcat
      661 gccgtgctgt atgtcggcct cgcctatctc gcatggttcg gcctgagcgc gctgcgccgc
      721 gcgttcaagc ccggccacga aacgctcgat gcgacgggcg cggttgccgc gccgcccgcg
      781 cagcgcgcct ggccgatcgt gctgatgacg ctcgcgttca catggctcaa cccgcacgtc
      841 tatctggaca cgttcctgct gatcggcacg gccggcgcgc gcgagccgga aggcgcgcgc
      901 ggcgcgttcg cgatcggcgc gatggcggtc agcgcggtat ggttcgtcgc gctcggatac
      961 ggcgcgcgat tgctcgcgcc atggttcaag cgcgcgctcg cgtggcgcgt gctcgacggc
     1021 gcgatcggcg gcatggtgct gtttctcgcg gcggtgcagt tgcgctaacg cggcggatgc
     1081 gcgttggcgg tgccgacgcc atctgaagcg cggacggcgg cacgcggcgc acggcggcga
     1141 gcgagccacg agcgtcatgg ccgcacgccc tgagggaatc ggccagccgg cgcacgcggt
     1201 cggcgcaagc ggcgccgccg agccgcgccc gtcatgccgc tacagcgcta ccaggtggac
     1261 gaagcgcgtc gtggccgcgc ggtcttcgat cacatgcaga ggccggcaac cggccgcccg
     1321 atacatggcg agccacgcgt cggcattggg cggcggcacg ccttgccccg atatgagttg
     1381 cgcatagtcg tgcagcagcg tctcgcggtg cggcgtttga aaaccgtggc cgaggcggcc
     1441 gtagtagtcc gcgatcacga gcaggcggtc gggcagcagg cggcgcatgc gtcgaagcca
     1501 ggcctgcgcg cgggacgtgc cgccgcgaaa catctcgttg acgaactggc tcgcgacgac
     1561 gttgcgcacg cgcgccagca cgcgcggcgg aatcgacgcg ccgggatgcc tgccgtcgcc
     1621 ctgaaacacc ttcacgcgcg ccgcgattcc cgcttgcctc gcccgcatcc gggcggcctt
     1681 gcacatcgat ggattgcgat ccagccccca cccgacgaat tcacgatcgt ccgccgccag
     1741 cgcgtgcagc agcgtgccgg cgccgcatcc gagatccagt acgtgattca ggtgcaattg
     1801 gctgatgatc gcggcgagcg ccccgccgcc cgcctcgctc gcctcgaagg cgcgcgcgtg
     1861 cgcgacgagg tcgactgtcg tgcccgccag cgccggccgc cggagcagcg tcgccagccg
     1921 cgaagcgtta tcggcatagg cgccggcata caggttcagc aggaaacgcg cttgcgccgt
     1981 gtagtgctcg gaagccgcga atcggtcgcc ggtcttgcgg atcaggttcg ttctcgatgc
     2041 cgcgtattcg aggacgcctc gcaacaactc gggatcgaga tcgcacgcgg cgctcagcgt
     2101 ttgcgccgtc gtcggctccg ccatcgcatc cacaacgccg agcgtttgca gcgcggcgac
     2161 ggcatgcgcc agttgaagac cttccgcgag atccagcgcg gaccagcgtt cgagtttccg
     2221 acgagccatg agcgaaatga atttagcggg ccggcaccaa aatgcctctg cgcagcagcg
     2281 gctcgaagat cgaagcgtcc acgccgtcat tgcgggtgac gtgcgccagc cattgcgcag
     2341 cggcgcggca cgaacggggc gccctgaaca gatcgaggat cgcggcaatg ccggactcca
     2401 cccaatagcc ggtgacggct tcgccgtccg cgcggacgat ataggcgccg agcccgccga
     2461 catccgcgtc gccgagcgcc ggcgcaccgt cgttcgagcg ccggagccga tcgaccaacg
     2521 cctcgcaatt gtggatgtcc tccagcaggc cgacgttttc ggccagcatg caaggccggt
     2581 ccggctcata gggcccttcg gcgacgacgt ccgccggagc gcgggccgcg gacagccgca
     2641 acgcgtaccg gaaaagagaa tgcaggtgat ggccgcggcc gaatcgcgcg gccacgaaag
     2701 cctccagccc cgccgcatcc ggcaacgcgc cgggcgacgt ttcgtccgcg aaccgtcgcc
     2761 atgccgatat cagccggctc agccgtcctc cgaattcccg caaagcgtac gcgaggatga
     2821 tcggccccgc gcggcgcagc acatccaccg cctcgacggc aaagatgtgt ctcgcgcgcg
     2881 gcagcgcggc ggggtagtaa tagtaggttt gaaatatgtc gggatggtcg agcacctcgc
     2941 gctcgtcctc cggacccagc aggcgcgtgt tgtaaggccc gccatagccg tcgtaggcga
     3001 tctgcgcccc gtgttcgtcg aacagcggcg tgccgggctc cggcgcgagc atgtgcagtt
     3061 gcgtcaggca ggacggccgc cgcgcgcagc gcccgatcat gtccagcgtg tcgtcctggt
     3121 cctgcccgct ttcctgcgga tagccggtga tgaacgacgc ggtcgtctcg attccgaacg
     3181 aatccgccgc ggccagaatg ggctcgacca gccgcaagtc caaccgcttc cggcagattg
     3241 tctgcatgcg ttcggagccg gtctccacgc cgaaataaag attcacgcag cccgcgtcgg
     3301 ccatcttcct cagcaatgct tcgtcgacgc aatcgatccg cgccgacgcg cgccaccggt
     3361 agccgcgccc ggccaccgcc tcgcaaaacg ccatcacctt gtggcgattc acggtaaaca
     3421 tgtcatggtc gagcttgaag tcggacacgc gatagcgccg atgaaggatg tccagctcgt
     3481 gcaccaggcg ctcggcggac ttgaggcgaa agctgcgctg aaagaagccc gccgtcgaac
     3541 agaacgtgca tgcgaacgga cagccgcggc cggcctcgat gcgcagcatc gagaggccca
     3601 gatcgtcgac cggataatgg tcgtacgacg cgatcggcag ccggtccagt tcctcgacct
     3661 tgggcttgcc gtcggtgaag cgcaacggcg caccgcggcc cgtcgcgcgc cagctcaagc
     3721 cggggataac atcgaatgtc cgctgctcga ggcagtcgag cacggcggga aggatctcgt
     3781 ccgcttcgta gcgaacgatg atgtcgaact gcgggaatcg ctcgaggatc tgccgatgca
     3841 gcatcgtcgc gtgcgggccg ccgagcagga ccggcaaatc gggctcgcgc cgcttcagca
     3901 gcgccgcgac attgagcgcg aacagaaaac tgcagccgag cgcggtgaag ccgaccgcgt
     3961 ccgggcgctc ggcgaggatc gcgcggctcg cccgctcgta aagcgtcgca tcgtaagcga
     4021 gcgtgccgtc ccggatcagt cgcttcggat catagatccg cacgcgatgg ccgtcggcct
     4081 cggcgaccgc ggccagcgcg agcagcccga gatgcggatg cacgtcgaga taggcgagcg
     4141 agccctcttc cggcatgacg agattgtcga cgagcagcag cttcatgccg cggcgcatcc
     4201 gagcggccgc gcgaatcgca cgatcccgat gccggccagt tggtcgagcg aactcaggaa
     4261 ctgcggcagc agacggcgat tgccgagcaa ccgatggcag atgtcggcgg cggatcgcct
     4321 gccgtcgatc gcttcgatcg cgcagtaacc gagcgtatcg acccgcagca accgtatttc
     4381 gcccgtttcg gcggagcgcc aatagcagca gtaagtgtcg acggcgggaa ccgcgtcgag
     4441 cggcggctcc ttctgatgaa gcgccgccgc cagatcgtca ggattgcagc gcatcacgtg
     4501 ttgaacgacg gagccgttga gcgtcggaat gccgccggcc gtcgcgcgcg cccgatgacg
     4561 cgcatcggga agcggcgcgt cgcccgcttg ccgcaggcat gcgagcgcgt attcatggcg
     4621 caggatgtcc cacagcagaa cgtgattgcg ttgcgcggga tcgagccatc tatcgagaaa
     4681 cgcaatcaga tcgcgcgcgc gctcctgcgg cgtggcggcg aatacgtggc cgctctccgc
     4741 gcagtccgac gcatacgcgg cgaatacctc gatctcgagg ccgaccacgc tcagcagcct
     4801 gaagctcagc ggaaaatctc cgcgcaggcc gttgtggcgc accttcaggc tcaacccgac
     4861 gaaatgctcc aatgcgcgcc aatcgagatc gccgggcgcg atgcccgcgc cgcgcagcag
     4921 gtccggatgc cgccgccaac gctcgatgag ctgcggatcc ttggtgctgg ccgcgatcag
     4981 cgcgtgtatc gtccttgcgt tcatgcaagc cccatcagcg cgcgcgcgcg ttcgatgtgg
     5041 ctcgtgagga ccgacaacgg gaagtatggc gaccagttgt attcgaaggt cagcgcgcga
     5101 ggccgcgcga cggcgagcag gcgctcgagc agttcgaaga ggatcggcga cgccggcagc
     5161 gaatgatcgt cccaggccgc gccggactgg atgctcatgc cggacaggtg aacttcgacc
     5221 acgcgctcga gcggcagccg ggcgaattcc cggtgcacgt cggcgcccgt gttgtacgcc
     5281 gtgatcagaa aatggctcag atccaggagc aagccgacat cgcaccggtc cgcgatcgcg
     5341 cgaatgaagt cggtcatgcc catcgtgctg ccgggaatcg aaaagtattg gggcgaattt
     5401 tccacgatca gcggaaccgg aaagcgcgga gcgagcacgg cgagattatc ggtgacgcgc
     5461 cgcaccgtct cgtcgctcaa ctgagggcac agcgtgtaag acagcgacgt gggctcatgg
     5521 gtcccaccca gcgcctcgcc gatgggatcg gccgagatgt acgcgagatg ttcgccgatc
     5581 cacggcgtgc cggtgcgcac ggccgtgcgc tcgattgcgt cgacggtcga atcgtcgggc
     5641 ggcacgaatc ccgcaacgga caagctggcg cagtgcagaa cgaacggaat ggtttgctga
     5701 agctcggcaa tcgtcggcga gaatcgcaag agctcgaacg gcacttcgat gtaatcgacg
     5761 agatgcgcat gccgtgcggc gaattccgcc atgccgggcc cgtacgcaag gccgatccgg
     5821 ggtgccccgg catcgcgatc gaacgctttc ataggcttcc cgcgccttca ggcgccgaac
     5881 cgcagcagtc cagatttatc gcccgccata gcgcgagcgc acgccgaacg cctcgcgttc
     5941 ggcggcgcgg cttctctgcg aagcccgagc atcgaggcga cgagaatgcg gcgcggccgg
     6001 caaggcccgc gcgcgccgcc ggacgtcagt cgtagagata gccctcgccc atgcggccgc
     6061 cggcgagatc caccttgctc atcgtgatcg tcagcttgcc gttttccagg catttctgga
     6121 tggccttgat ctttttcgcg tccaccggca tatccagcgt catcttgggc agcttccctt
     6181 cgatggaaac ggaaacgccc tttgtttttt tgggtttcgt ggccatgatc gttcctttgt
     6241 tgtgggcagg acgcgacgct cagctgtgag tgtggaataa cccccgaaaa tcgccatgcg
     6301 aatcgatgac cgaaaaaatc ccgtcgggtg tgcatgccga tttgcttctg attaaaggat
     6361 gcgccccggt aaatgtcaag aaagccccgg cgcgaaatgg aacagctcgg cgacaggccc
     6421 gcgccatttc ggcgacggcg tgtcgcggcg ttcatcgacg gggtgcgcca tgagcgtcgg
     6481 gtcgcctttc ggcatcagca tgatatgaat gcgccagtcg ataaactgtt cgatatccaa
     6541 aacgaaatcg aatcgcgata acagcgtcga cagcacttcg ccaatcagcg acagcgcgag
     6601 attccgcccc gcgcagaaat ggccgccggc gccgaatgga aaatactcgt acggcgacgg
     6661 cctcgccgtc tcccatcggg acggcaagaa tgcatgcggg cgcgcaaacg cgttggcctc
     6721 gcggtgcacg aggaacgggc acacgacaat ctcgcagcgc gcgggaagcg cgacgccttg
     6781 caacgaaacc gcctgcgtcg tggcgcggac catcagcgcg ttgggcgtca ggagccgaag
     6841 cgtctcgttg atgacgcgct cgagcaatgg cgcgtcgtac ggcgaagccg gcgacgatgc
     6901 gcgggcggcg ctttccgcgc gaagcgcacg ccgcagatcg ggcaactgcg acagaacgag
     6961 cagcagccac gccagcgata tcgccaccgg ctccgtgctc gacacgaaca tcacgttggc
     7021 gtggccgacg atctcatcct cggaaagcgc gggcgaaccc ggctggccgg ccgtcgccag
     7081 ccgctggagc agccccgcgc actcgccggc gggcgccttt cgataccgcc ggatccgcgc
     7141 ccgcaacgcg cgatcaagtt gccgcccggc gccgatcagc gcgtcccggt actgcaacgg
     7201 atcgtgcgcg ttgggcgaag acgcctcgcg ccgcaacgtg aaatacgcgc ttagcagaaa
     7261 cgtcagttcg gcatcctcct cacactgcgg cccaagaaaa agacgggacg ccaacttggc
     7321 tgtcagctcg cgcattcggc tcacgacgga aatccggccg tccccgttca gcatttcgca
     7381 aaagcggctc aaatccgttt gcattccgga tcgatgcgcg tcgaacagcg cgcggttggc
     7441 cgttcccgtc aggaggcgct tgtgccggcc gtgctccggc tcgcgcatgc tgtgcaagcc
     7501 gcggttgagg ttgaccagat tcttcggcaa cgccatcttc gccgccgctg acatcggcag
     7561 cgcgaaggtg tccgtctcgg tcaagacttg tcgaagccga tgttcgccga acacggcgat
     7621 cacgccgctg caatcgctcg cgcgagaaaa aatcggaccg tgctcccgca gcacgaagac
     7681 gtcgccgcat tgcgcgcgcg ctcgtgcgag aaaagcgagc ggatccgcaa agagcgaatc
     7741 ggtgctgccg actcgcggaa gcgacagagg gaggccattc atttgcgcgc tgtgtccgtg
     7801 aacgattcgt gccgatcgtg gctacgccgg ttgatcgcta cagcatggct gccgtcacag
     7861 tatcacgtct tccgaagccg cccggccggt accgcccgag cgcgaacgca acgccgccgc
     7921 gcaatacggc atgccccggg acggataaac atccgccatc gaacgaacgg gccagggccc
     7981 gggttcgcca tgccgccttc ggccgtcgcc gtcgccccac ccttgcacgc ggcattccgg
     8041 catcgtcgcg ccctccgcgt gtcggtacca ggcgggacat cgcccgtccg gccatcgcac
     8101 tccgccccat cgttccccgt ttaaattccg ttgactttat atctttcgat atatatcttt
     8161 agatacatct aaagacacag aaggcggaca tgcgtcacgc tcaccgtttt tcgcgccgcg
     8221 aatgcggcat gtccgattcg ttcggacaca gcccgttcga gatgtttcgt cagatgttcg
     8281 gcggccatcg ccgcggcggc ggccggcatg gcttcggccc attcggcggc ttcggcggcg
     8341 gcggccccgg tgggttcggc ggcttcggag acgacgcgat gccgcgcggc cgtcaattca
     8401 gcgccgacga tctgcagttg ctgctgctcg cgctgctcgc cgagcagccg agccacggct
     8461 acgagctgat caaggcgctc gacacgcgct cgagcggctt ctacgcgccg agccccggca
     8521 tggtgtatcc ggcgctcacg tatctggaag agctcggcta cgtgacggtg caggccgaag
     8581 gcaaccgcaa gcgttacgcg ctcgccgacg cgggccgcgc gcatctcgac gcgcagcgtg
     8641 aacgcgtcga gatgctgttc gcgaagctcg cgcatctcgg ccgcaagatg gaattcatgc
     8701 gtcgcgcgtt cgccggcgaa gcgcccgccg cgcccgagga cgacacgcgc ggctggctgc
     8761 cggaattcgt cgaagcacgg atggcgctca agcaagcgct ctttcgcagg agcggcgcaa
     8821 acgccgacga gcaacgccgc atcgcggcca ttttgcggcg cgccgtcgcc gacatcgaag
     8881 gccgcgcgca aggctgaatc gcgccgtttc cggctcatca aggagaaccg atcgacatga
     8941 cccaaccgac taccgagcgc gcggtcgtac gcgtgcgtca tacgctgaaa ttccgcctgc
     9001 tgcaggtcaa gcgcgtcgtc gacgtgacgc cgcatctgaa gcgcgtgacg ctggcaggcg
     9061 acgaactcga cgatttcgaa tcggcgtcgt tcgacgatca cgtgaaagtc ttcttcccgc
     9121 cgcccggcga agacgcaccc gcgcgccccg aattcggccc gaacggcatc gtgtttccgc
     9181 aggacaggcc gcgccccgtc gcacgcgatt tcacgccgcg ccgctatgat cgcgccgcgc
     9241 gcgagctcga tctcgatttc gtgctgcatc atccggggcc ggcttcgcag tgggccgctc
     9301 aggcgcgcgt cgggcaaacg ctcggcatcg gcgggccgcg cggttcgttc gtgattccga
     9361 aggatttcga ctggcatctg ctgatcggcg acgacaccgc attgcccgcc gtcgcgcgcc
     9421 ggctcgagga attgccggcc ggcacgcgcg tcgcggcggt gctcgaagtg gccgatccgt
     9481 cggcgcagat cgcattcgaa accgatgcga acctgtatgt ggtatggcga catcgcggcg
     9541 atgcggcgag cggcgagcgc ggcgcgagcc ggctggtcga cgcattgcgc gaactgccgc
     9601 tgccggcggg cgaaggctac gtatgggcgg cgggcgaggc ggccgcgatg cgcggcgtgc
     9661 gcgagcatct gtgccgcgag cgcggcgtcg cgaaatcgcg gattcgcgca tccgcttact
     9721 ggaagcacgg tgcgatcgcc gtgcacgaat cgctcgaaga ctgatccgcg gccggccgct
     9781 gcgcgcgcga atatcgcccg gcgattcacg cgcgcgatcc aagcgcacgg cgtcgccggc
     9841 acccgcatgc agtattgcaa tgcacgcgat agcggccggc aggcagcaca cgcgcgtacg
     9901 cctcgaccgg ccatcgccga aagcgaaagc caaaaccgca aaccccttca cgccgccgct
     9961 acgttcgccg cgtcgcgttt tcgccgtttc gtatgagctg aatcgcgcgc gtaccgctgt
    10021 cgcatcgcat cggcttgtct tgattgtctt gaaatagccg cgcaccgcca cgctcgcgat
    10081 acgtcgctca tggcaagcgg ctctggttcg cgcccgatcg caccgcaccg ccgcgccgct
    10141 cgagcgcagc cgcgccgcgc tgacccatcg catcgcacgc cttcgctgcc attccgcgcg
    10201 ccgacaaagc cgtttcgttc gcttctccgc cgttttcccg ccgatcaacg ccgcagcgac
    10261 ggcggccggc gcgcgcgttc aggttccatc gtccgcttgc atcgcgcgat gccctcgcat
    10321 ccgcgcgtct ccacacgagc ggagttgcat ccgcatcgcc cgtgtcctat acctatgtcg
    10381 acggcacatg gcgacgccga tggcggcaca acggggcgcg cgccgccgtg accggatagg
    10441 gaaaagcacg gcgcgcaccg cggtattttc gcgtgtcgaa cgagaacgag accgcgcggc
    10501 ttcgcgcgtc gccacccgga tacgcgtcca tgactcgcca ccacacccac gccggcattc
    10561 cgagaacgct caaaatcgtc ggctattgcg ggctcgccgc aatcgcgctc gcgctgttcg
    10621 tcgcgatgtg cgcgatcctc aaagacagcg cgctcgatcg cgaagccgcg cgccagttgc
    10681 agaacgggaa cgtcgaattg cacggcggcg ccgatgtcga gccgggctcg acgaacgacg
    10741 ccgcccaggc gagaacgccg ggttgatccg gattcctgcc gatcagcgtc agtcgatgcg
    10801 gatcggccgc tcgtgcaatc gcgtgaacgc gaccgcgtgc accgattcgc cg
//
