LOCUS       NZ_KL573590             4522 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces baarnensis strain NRRL B-2842 P144_Doro1_scaffold64,
            whole genome shotgun sequence.
ACCESSION   NZ_KL573590
VERSION     NZ_KL573590.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces baarnensis
  ORGANISM  Streptomyces baarnensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Doroghazi,J.R., Albright,J.C., Goering,A.W., Ju,K.-S., Haines,R.R.,
            Tchalukov,K.A., Labeda,D.P., Kelleher,N.L. and Metcalf,W.W.
  TITLE     A Roadmap for Natural Product Discovery Based on Large-Scale
            Genomics and Metabolomics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Doroghazi,J.R., Ju,K.-S. and Metcalf,W.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2014) Institute for Genomic Biology, University of
            Illinois at Urbana-Champaign, 3109 IGB, MC-195, Urbana, IL 61801,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: IDBA UD v. 1.0.9
            Genome Coverage                   :: 74x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 02:19:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,395
            CDSs (total)                      :: 7,345
            Genes (coding)                    :: 7,173
            CDSs (with protein)               :: 7,173
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 55 of 173
            Pseudo Genes (incomplete)         :: 138 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 27 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4522
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KL573590.1.
            Bacteria available from NRRL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4522
                     /collection_date="2010"
                     /culture_collection="NRRL:B-2842"
                     /db_xref="taxon:66872"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces baarnensis"
                     /strain="NRRL B-2842"
                     /submitter_seqid="P144_Doro1_scaffold64"
     region          1..4522
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..4522
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4522
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2761:>4522](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2762..>4522
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            12..>760
                     /locus_tag="BG84_RS1000000139090"
     CDS             12..>760
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Epimerization (1-249). E-value: 9.3e-90.
                     Score: 293.3. Matches aSDomain:
                     nrpspksdomains_BG84_RS1000000139090_Epimerization.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="BG84_RS1000000139090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_158688363.1"
                     /sec_met_domain="Condensation (E-value: 9.1e-24, bitscore:
                     71.1, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGEVVVPPVVEWLRGLGSGVSGFHQSVLVSVPGGVGLGALAAAVR
                     GVVDHHDALRLRLLVGADGGWGLEVGAVGSVPVEGCVRRVDVSGGDGGVLSELVRVEAD
                     AARGRLDPVSGVMVQVVWFDAGVGRAGRLLWVVHHLAVDGVSWRVLLPDLEAAWRQVAA
                     GGPVALEPVGTSYRAWAGMLPELAADRAGELALWQGVLAEPDGLLGSRALDPVVDVAGT
                     AGSVTVEVPAEVSQGLLSWVPGVWGAGV"
     aSDomain        15..758
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BG84_RS1000000139090_Epimerizati
                     on.1"
                     /evalue="9.30E-90"
                     /label="BG84_RS1000000139090_Epimerization.1"
                     /locus_tag="BG84_RS1000000139090"
                     /protein_end="249"
                     /protein_start="1"
                     /score="293.3"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLAVDG"
                     /tool="antismash"
                     /translation="GEVVVPPVVEWLRGLGSGVSGFHQSVLVSVPGGVGLGALAAAVRG
                     VVDHHDALRLRLLVGADGGWGLEVGAVGSVPVEGCVRRVDVSGGDGGVLSELVRVEADA
                     ARGRLDPVSGVMVQVVWFDAGVGRAGRLLWVVHHLAVDGVSWRVLLPDLEAAWRQVAAG
                     GPVALEPVGTSYRAWAGMLPELAADRAGELALWQGVLAEPDGLLGSRALDPVVDVAGTA
                     GSVTVEVPAEVSQGLLSWVPGVWGAG"
     CDS_motif       108..185
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS1000000139090_0001"
                     /evalue="1.60E-06"
                     /label="C2_starter_024-063"
                     /locus_tag="BG84_RS1000000139090"
                     /protein_end="58"
                     /protein_start="32"
                     /score="20.8"
                     /tool="antismash"
                     /translation="GGVGLGALAAAVRGVVDHHDALRLRL"
     CDS_motif       408..473
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS1000000139090_0002"
                     /evalue="4.40E-11"
                     /label="NRPS-E2"
                     /locus_tag="BG84_RS1000000139090"
                     /protein_end="154"
                     /protein_start="132"
                     /score="34.9"
                     /tool="antismash"
                     /translation="LLWVVHHLAVDGVSWRVLLPDL"
     assembly_gap    761..1074
                     /estimated_length=314
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1212..1516
                     /estimated_length=305
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1694..1963
                     /estimated_length=270
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2138..2480
                     /estimated_length=343
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2627..2759
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2760..>4522
                     /locus_tag="BG84_RS0137100"
     CDS             2762..>4522
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_DCL (53-363). E-value:
                     5.7e-108. Score: 352.5. Matches aSDomain:
                     nrpspksdomains_BG84_RS0137100_Condensation_DCL.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="BG84_RS0137100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_158688365.1"
                     /sec_met_domain="Condensation (E-value: 2.6e-64, bitscore:
                     204.2, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 7.2e-12, bitscore:
                     31.6, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEELARLWVGVLEALVQEAGEPGAGGLTPSDVLAEVSQAEIEAL
                     EATYPGLDDVLPLAPLQEGLHFHALDEDAGSAAYTVQIGLDLEGGLDLRAMRHAAEALL
                     ERHAGLRAGFVHEGLDAPVQVIASEVELPWSQRDVSELDAPERDAALGRILDEDRARRF
                     DPTRPPLLRFTAIRLGENRHHLVLTYDHLLLDGWSTAVLMRELFALYANGCDTTALPRV
                     TAFREHLGRLAEQDRPAAEEAWRTALAGLDEPTLLAEAFGTAGSAGSADPADPASVAAP
                     VSEQLRVELTEEQTRAVAARARALGVTLNTVVQGSWGLTLARLTGREDVVFGATVSGRP
                     ADLPGIEDMVGLFINTLPVRVRVRPADSLAGLLTAVQDEQARLMPHQHVRLTDVQRLAG
                     HGELFDSIAVFENYPVDALDLGTPDGRLRVTGIDGGDTTHYPLTLIAVPGDRLHLRLGY
                     RTDLIGEERAGRFLNGLLAVLDAVAAGTDRPTGRIDLLTAAEREQLLVHRNDTAAAVPD
                     GTVADAFQRQAAATPDAVAVRFEGAAVSYAELDDRASRLARLLVARGAGPEQFVAVAME
                     RSADLLVAVLA"
     aSDomain        2922..3851
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BG84_RS0137100_Condensation_DCL.
                     1"
                     /domain_subtypes="Condensation_DCL"
                     /evalue="5.70E-108"
                     /label="BG84_RS0137100_Condensation_DCL.1"
                     /locus_tag="BG84_RS0137100"
                     /protein_end="363"
                     /protein_start="53"
                     /score="352.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: DHLLLDG"
                     /tool="antismash"
                     /translation="DVLPLAPLQEGLHFHALDEDAGSAAYTVQIGLDLEGGLDLRAMRH
                     AAEALLERHAGLRAGFVHEGLDAPVQVIASEVELPWSQRDVSELDAPERDAALGRILDE
                     DRARRFDPTRPPLLRFTAIRLGENRHHLVLTYDHLLLDGWSTAVLMRELFALYANGCDT
                     TALPRVTAFREHLGRLAEQDRPAAEEAWRTALAGLDEPTLLAEAFGTAGSAGSADPADP
                     ASVAAPVSEQLRVELTEEQTRAVAARARALGVTLNTVVQGSWGLTLARLTGREDVVFGA
                     TVSGRPADLPGIEDMVGLFINTLPVRVRV"
     CDS_motif       2931..2966
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137100_0001"
                     /evalue="8.80E-04"
                     /label="C1_dual_004-017"
                     /locus_tag="BG84_RS0137100"
                     /protein_end="68"
                     /protein_start="56"
                     /score="12.3"
                     /tool="antismash"
                     /translation="PLAPLQEGLHFH"
     CDS_motif       2991..3110
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137100_0002"
                     /evalue="1.30E-13"
                     /label="C2_DCL_024-062"
                     /locus_tag="BG84_RS0137100"
                     /protein_end="116"
                     /protein_start="76"
                     /score="43.2"
                     /tool="antismash"
                     /translation="AAYTVQIGLDLEGGLDLRAMRHAAEALLERHAGLRAGFVH"
     CDS_motif       3333..3392
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137100_0003"
                     /evalue="5.00E-10"
                     /label="C3_DCL_135-156"
                     /locus_tag="BG84_RS0137100"
                     /protein_end="210"
                     /protein_start="190"
                     /score="31.8"
                     /tool="antismash"
                     /translation="HLLLDGWSTAVLMRELFALY"
     CDS_motif       3747..3842
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137100_0004"
                     /evalue="4.60E-23"
                     /label="C5_DCL_263-294"
                     /locus_tag="BG84_RS0137100"
                     /protein_end="360"
                     /protein_start="328"
                     /score="73.6"
                     /tool="antismash"
                     /translation="DVVFGATVSGRPADLPGIEDMVGLFINTLPVR"
     CDS_motif       3891..4004
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137100_0005"
                     /evalue="3.20E-19"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="BG84_RS0137100"
                     /protein_end="414"
                     /protein_start="376"
                     /score="61.4"
                     /tool="antismash"
                     /translation="QDEQARLMPHQHVRLTDVQRLAGHGELFDSIAVFENYP"
ORIGIN
        1 ccggtggtgg tgtgggtgag gtggtggtgc ctccggtggt ggagtggctg cgggggctgg
       61 gctctggtgt gagtggtttt catcagtcgg tgttggtgtc ggtgccgggt ggtgtgggtc
      121 tgggtgcgtt ggctgcggcg gttcggggtg tggtggatca tcacgacgct ttgcggttgc
      181 ggttgttggt cggggctgat ggtggttggg gtcttgaggt cggggccgtt ggttcggttc
      241 cggtcgaggg gtgtgtgcgg cgggttgatg tgtcgggtgg ggacgggggt gtgttgtcgg
      301 agttggtccg tgtggaggcg gatgcggctc gtgggcggtt ggatcctgtg tcgggtgtga
      361 tggtgcaggt ggtgtggttc gacgcgggtg tggggcgtgc gggccggttg ttgtgggtgg
      421 tgcatcacct ggcggtcgac ggtgtgtcgt ggcgggtgtt gctgccggac ctggaagcgg
      481 cgtggcggca ggttgcggcc ggtgggccgg tggcgttgga gccggtgggg acgtcgtacc
      541 gggcctgggc cgggatgctg ccggagctgg ccgcggaccg tgccggtgag ctggcgctgt
      601 ggcagggcgt attggccgag cctgatgggc tgttgggttc gcgtgcgctg gacccggtgg
      661 tggatgtcgc gggcactgcg ggttcggtga cggtggaagt tccggccgag gtgtcgcagg
      721 ggttgctgtc ctgggttccc ggtgtgtggg gcgcgggagt nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggtca
     1081 tgtgctggtg gatcttgagg ggcatggccg tgagcagttg gtggacggct gggatctgtc
     1141 gcggacggtc gggtggttca cgagccggta ccccgtgcgg ctggacgtcg acggggtgga
     1201 cgtcggcgct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnngcgg tgaagagggt gaaggaggcc gtgcgggctc tgccggaccg
     1561 gggtgtcggg ttcggtctgc tgcggtacgt ggagcccggc gcctctgagg cgctgtcggc
     1621 gttggcggaa ccgcaggtcg ggttcaacta cctgggccgc ttccggtccg gcggtgccgg
     1681 cggtgagggt gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtccggt gaggacgact
     1981 ggctgcccgt cggtgagtcg ggaggcgtcg gtggtggtgt cgatccgtcg atgcggatgc
     2041 cgcacgcggt ggaggtcaac gccctggcgc tggacggcgc gagtggttcg gtgctgtcgg
     2101 cggtctgggc gtggcctggt ggtgtgttcg gtgacggnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn tgaggtcgag gagctggccc ggttgtgggt cggtgtcctg
     2521 gaggctttgg tggccggggc cggtgagccg ggtgccggtg gtctgacgcc ctcggatgtg
     2581 ctggcggagg tgtcgcaggc ggagatcgag gcgctggagg cgacgtnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     2761 gaggtcgagg agctggcccg tctgtgggtc ggtgtcctgg aggccttggt ccaggaggcc
     2821 ggtgagcccg gtgccggtgg tctgacccct tccgacgtgc tggcggaggt gtcgcaggcc
     2881 gagatcgagg cgctggaggc gacgtatccg gggctggacg acgtactgcc gctcgcaccg
     2941 ttgcaggaag gactccactt ccacgcgctc gacgaggacg cgggctccgc cgcgtacacc
     3001 gtgcagatcg gcctcgatct cgaaggcggc ctcgacctcc gcgcgatgcg ccacgccgcc
     3061 gaagcgctcc tggaacggca cgcggggctg cgcgccggat tcgtgcacga aggtctggac
     3121 gctccggtcc aggtcatcgc ctccgaggtc gaactgccct ggtcccagcg ggatgtgagc
     3181 gagctggacg ccccggagcg cgacgcggct ctcggccgga tcctggacga ggaccgcgcg
     3241 cgccgcttcg atccgaccag gccgccgctg ctccgcttca ccgcgatccg cctcggcgag
     3301 aaccgccacc acctggtcct cacctacgat cacctgctcc tcgacggctg gtcgaccgcc
     3361 gtcctgatgc gcgagctgtt cgccctgtac gccaacggct gcgacaccac cgctctgccc
     3421 cgggtcacgg ccttccgcga gcacctcggc cggctggccg agcaggaccg cccggccgcc
     3481 gaggaggcct ggcgcaccgc gctcgccgga ctcgacgagc cgacgctcct cgccgaagcc
     3541 ttcggcaccg ccggttccgc cggttccgcc gacccggccg atccggcctc cgtggcagcc
     3601 ccggtctccg aacagctccg cgtcgagctc accgaggagc agacgcgggc cgtcgccgcg
     3661 cgtgcccgcg cgctcggcgt cacgctcaac accgtggtgc agggcagctg gggtctgacg
     3721 ctggcccggc tcaccggccg cgaggacgtc gtcttcggcg ccaccgtctc cggccgtccc
     3781 gccgatctgc ccggcatcga ggacatggtg ggcctcttca tcaacaccct gccggtacgc
     3841 gtccgggtcc gccccgccga cagcctcgcc ggtctcctca cggccgtgca ggacgaacag
     3901 gccaggctga tgccgcacca gcacgtccgg ctgaccgatg tgcagcggct ggccggacac
     3961 ggcgaactct tcgactccat cgccgtgttc gagaactacc cggtcgacgc gctcgacctc
     4021 ggcactccgg acggccggct ccgcgtcacc ggtatcgacg gcggtgacac cacgcactat
     4081 ccgctcaccc tgatcgccgt tcccggcgac cgtctgcacc tgcgcctcgg ctaccgcacc
     4141 gacctcatcg gcgaggagcg cgccgggcgc ttcctgaacg gactgctcgc cgtcctcgac
     4201 gcggtcgccg ccggcaccga ccggccgacc ggccggatcg acctgctcac cgcggccgaa
     4261 cgcgagcagc tcctcgtcca ccgcaacgac acggcggcag cggtgccgga cggcacggtc
     4321 gccgacgcgt tccagcgcca ggccgccgcc accccggacg ccgtggccgt ccgcttcgag
     4381 ggcgccgccg tgtcctacgc cgagctggac gaccgtgcgt cgcggctggc caggctgctc
     4441 gtcgcccggg gggcgggccc ggagcagttc gtcgcggtcg ccatggagcg ctcggccgat
     4501 ctgctcgtcg ccgtactggc gg
//
