LOCUS       NZ_KL573589             3032 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces baarnensis strain NRRL B-2842 P144_Doro1_scaffold63,
            whole genome shotgun sequence.
ACCESSION   NZ_KL573589
VERSION     NZ_KL573589.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces baarnensis
  ORGANISM  Streptomyces baarnensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Doroghazi,J.R., Albright,J.C., Goering,A.W., Ju,K.-S., Haines,R.R.,
            Tchalukov,K.A., Labeda,D.P., Kelleher,N.L. and Metcalf,W.W.
  TITLE     A Roadmap for Natural Product Discovery Based on Large-Scale
            Genomics and Metabolomics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Doroghazi,J.R., Ju,K.-S. and Metcalf,W.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2014) Institute for Genomic Biology, University of
            Illinois at Urbana-Champaign, 3109 IGB, MC-195, Urbana, IL 61801,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: IDBA UD v. 1.0.9
            Genome Coverage                   :: 74x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 02:19:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,395
            CDSs (total)                      :: 7,345
            Genes (coding)                    :: 7,173
            CDSs (with protein)               :: 7,173
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 55 of 173
            Pseudo Genes (incomplete)         :: 138 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 27 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3032
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KL573589.1.
            Bacteria available from NRRL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..>1851
                     /locus_tag="BG84_RS0137065"
     source          1..3032
                     /collection_date="2010"
                     /culture_collection="NRRL:B-2842"
                     /db_xref="taxon:66872"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces baarnensis"
                     /strain="NRRL B-2842"
                     /submitter_seqid="P144_Doro1_scaffold63"
     region          1..3032
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..3032
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..3032
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[4:>1851](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5..>1851
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             5..>1851
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_DCL (1-301). E-value:
                     1.6e-104. Score: 341.1. Matches aSDomain:
                     nrpspksdomains_BG84_RS0137065_Condensation_DCL.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="BG84_RS0137065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_032791333.1"
                     /sec_met_domain="Condensation (E-value: 4e-59, bitscore:
                     187.2, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 7e-30, bitscore:
                     90.9, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGLHFHALYDTESPDLYTIQLVFDLEGDLDTETMRRAAAALLRRR
                     PNLRAGFLNQESGRPVQVVPAVTEVPWTDVDLRGIGAVQQEDLADTILDEDRAHRFDPA
                     RPPLLRFTAIRLGDNRHRLVLTNHHLLWDGWSVPVVVRELFELYAHGGDGAHLAPAPQF
                     REHLDWLAAQDRPAAEEAWRTALAGLDEPTLLAEAFGTADSAGAVSSDERAPDSSGLPE
                     RLSVRLPQSFTAELHTWARSRGLTVNTVLQGAWALLLARLTGREDVVFGATVSGRPADL
                     PGVEDMTGLFSNTLPVRVALERARALSLVGLLEQIQDQQAELMDHHHVGLSGIAARTDL
                     GELFDTLLVFENYPLDPSTLDLPGTGLRATGARVRDAAHYPLSLAVVPGDELELRFDYR
                     PERFTADRVEAIGDRLHALLGLIVTEPDRPVGRVGVLTDAEAEQLVQDRRQSRRYVELL
                     PFPDLFEMQAARTPDTAAVVYEETEDHSVELTYAELDARANRLARLLIERGIGPEDIVG
                     LLLPRSVDQVVAVLAVAKAGAAYLPIDPAYPAERIAYVVGDARPVLLLVDAVTGRRLPE
                     LDTGTPALVLGGDIALRRPGHRPAADERRAQLTPDSPAYV"
     aSDomain        10..909
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BG84_RS0137065_Condensation_DCL.
                     1"
                     /domain_subtypes="Condensation_DCL"
                     /evalue="1.60E-104"
                     /label="BG84_RS0137065_Condensation_DCL.1"
                     /locus_tag="BG84_RS0137065"
                     /protein_end="301"
                     /protein_start="1"
                     /score="341.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHLLWDG"
                     /tool="antismash"
                     /translation="GLHFHALYDTESPDLYTIQLVFDLEGDLDTETMRRAAAALLRRRP
                     NLRAGFLNQESGRPVQVVPAVTEVPWTDVDLRGIGAVQQEDLADTILDEDRAHRFDPAR
                     PPLLRFTAIRLGDNRHRLVLTNHHLLWDGWSVPVVVRELFELYAHGGDGAHLAPAPQFR
                     EHLDWLAAQDRPAAEEAWRTALAGLDEPTLLAEAFGTADSAGAVSSDERAPDSSGLPER
                     LSVRLPQSFTAELHTWARSRGLTVNTVLQGAWALLLARLTGREDVVFGATVSGRPADLP
                     GVEDMTGLFSNTLPVRVAL"
     CDS_motif       49..165
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137065_0001"
                     /evalue="4.10E-13"
                     /label="C2_DCL_024-062"
                     /locus_tag="BG84_RS0137065"
                     /protein_end="53"
                     /protein_start="14"
                     /score="41.6"
                     /tool="antismash"
                     /translation="DLYTIQLVFDLEGDLDTETMRRAAAALLRRRPNLRAGFL"
     CDS_motif       385..450
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137065_0002"
                     /evalue="1.40E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="BG84_RS0137065"
                     /protein_end="148"
                     /protein_start="126"
                     /score="36.8"
                     /tool="antismash"
                     /translation="NHHLLWDGWSVPVVVRELFELY"
     CDS_motif       805..900
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137065_0003"
                     /evalue="4.20E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="BG84_RS0137065"
                     /protein_end="298"
                     /protein_start="266"
                     /score="64.2"
                     /tool="antismash"
                     /translation="DVVFGATVSGRPADLPGVEDMTGLFSNTLPVR"
     CDS_motif       955..1068
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137065_0004"
                     /evalue="5.80E-16"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="BG84_RS0137065"
                     /protein_end="354"
                     /protein_start="316"
                     /score="50.9"
                     /tool="antismash"
                     /translation="QDQQAELMDHHHVGLSGIAARTDLGELFDTLLVFENYP"
     CDS_motif       1597..1638
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137065_0005"
                     /evalue="2.70E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="BG84_RS0137065"
                     /protein_end="544"
                     /protein_start="530"
                     /score="17.3"
                     /tool="antismash"
                     /translation="LAVAKAGAAYLPID"
     assembly_gap    1852..2040
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2041..>2604
                     /locus_tag="BG84_RS0137070"
     CDS             2045..>2604
                     /NRPS_PKS="Domain: AMP-binding (1-186). E-value: 3.5e-39.
                     Score: 126.5. Matches aSDomain:
                     nrpspksdomains_BG84_RS0137070_AMP-binding.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018524445.1"
                     /locus_tag="BG84_RS0137070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AMP-binding protein"
                     /protein_id="WP_030089435.1"
                     /sec_met_domain="AMP-binding (E-value: 8.4e-43, bitscore:
                     133.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSGSTGRPKGVVVTHRGVANLAGSQIERFAVEEGSRILQVASPS
                     FDAAFSELCLALLSGSTLVLAPADRLLPGAGRSGLAGVIARQRITHATISPAALAALDD
                     DGLPAGMVLTVAGEACSAALVDRWSRDRRMINAYGPTETTVCATMSEPLAPSDRRTPPP
                     IGRAVWNTHAYVLDASLGPVVPGV"
     CDS_motif       2050..2094
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137070_0001"
                     /evalue="1.30E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="BG84_RS0137070"
                     /protein_end="16"
                     /protein_start="1"
                     /score="24.0"
                     /tool="antismash"
                     /translation="TSGSTGRPKGVVVTH"
     aSDomain        2050..>2604
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BG84_RS0137070_AMP-binding.1"
                     /evalue="3.50E-39"
                     /label="BG84_RS0137070_AMP-binding.1"
                     /locus_tag="BG84_RS0137070"
                     /protein_end="186"
                     /protein_start="1"
                     /score="126.5"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="TSGSTGRPKGVVVTHRGVANLAGSQIERFAVEEGSRILQVASPSF
                     DAAFSELCLALLSGSTLVLAPADRLLPGAGRSGLAGVIARQRITHATISPAALAALDDD
                     GLPAGMVLTVAGEACSAALVDRWSRDRRMINAYGPTETTVCATMSEPLAPSDRRTPPPI
                     GRAVWNTHAYVLDASLGPVVPG"
     CDS_motif       2314..2331
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137070_0002"
                     /evalue="9.90E+01"
                     /label="NRPS-A_a5"
                     /locus_tag="BG84_RS0137070"
                     /protein_end="95"
                     /protein_start="89"
                     /score="-3.1"
                     /tool="antismash"
                     /translation="THATIS"
     CDS_motif       2458..2484
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137070_0003"
                     /evalue="6.00E-04"
                     /label="NRPS-A_a5"
                     /locus_tag="BG84_RS0137070"
                     /protein_end="146"
                     /protein_start="137"
                     /score="12.2"
                     /tool="antismash"
                     /translation="YGPTETTVC"
     assembly_gap    2605..2894
                     /estimated_length=290
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
ORIGIN
        1 aggaaggact ccacttccac gcgctgtacg acaccgagtc gcccgacctc tacaccatcc
       61 agctcgtctt cgacctcgaa ggcgacctgg acacggagac gatgcgccgc gcggccgccg
      121 cgctgctgcg ccgccggccc aatctgcgcg ccggtttcct gaaccaggag tccggccggc
      181 cggtgcaggt ggtaccggcc gtcaccgagg tgccgtggac ggacgtcgac ctccgcggca
      241 tcggcgccgt ccagcaggag gacctggcgg acacgatcct ggacgaggac cgtgcccacc
      301 gcttcgatcc ggcgcggccg ccgctgctcc gcttcaccgc gatccgcctc ggcgacaacc
      361 gccaccgcct cgtcctcacc aaccaccacc tcctctggga cggctggtcg gtgccggtcg
      421 tcgtgcggga gctgttcgag ctgtacgcgc acggcggcga cggggcgcac ctcgctccgg
      481 ccccgcagtt ccgcgagcat ctcgactggc tggcagcgca ggaccgtccg gccgcagagg
      541 aggcctggcg caccgcgctc gccggactcg acgagccgac gctcctcgcc gaagccttcg
      601 gcaccgccga ctccgccggt gccgtgtctt ccgacgagcg ggcaccggac tcctccggcc
      661 tgccggagcg gctgtccgtc cggctgccgc agtccttcac cgccgagctg cacacctggg
      721 cccgctcgcg cggactcacc gtgaacaccg tcctccaggg tgcgtgggct ctgctgctcg
      781 cccggctcac cggccgtgag gacgtcgtct tcggcgccac cgtctccggc cggcccgccg
      841 atctgcccgg cgtcgaggac atgaccggac tgttcagcaa cacgctgccg gtacgcgtcg
      901 cactggagcg ggcccgggcc ctgtcgctgg tcgggctgct ggagcagatc caggaccagc
      961 aggccgagtt gatggatcat caccacgtcg ggctgtccgg catcgccgcg cggaccgacc
     1021 tcggcgagct cttcgacacc ctgctcgtct tcgagaacta tccgctcgac ccgtcgacgc
     1081 tcgacctgcc gggcaccgga ctgcgggcca ccggcgcgcg cgttcgcgac gccgcccact
     1141 atccgctctc gctcgccgta gtaccgggcg acgagctcga actccgcttc gactaccggc
     1201 cggagcgctt caccgccgac cgtgtcgagg ccatcggcga ccggctgcac gccctgctcg
     1261 gcctgatcgt gacggagccg gaccggcccg tcgggcgcgt cggcgtcctc accgacgccg
     1321 aggcggaaca gctggtccag gaccggcggc agtcccggcg gtacgtcgaa ctcctgccgt
     1381 tcccggacct gttcgagatg caggcggcac ggacgccgga cacggcggcc gtggtgtacg
     1441 aggagacgga ggaccactcc gtcgagctga cgtacgccga actcgacgca cgggccaatc
     1501 gcctggcccg gctgctcatc gagcgcggca tcggcccgga ggacatcgtc ggcctgctgc
     1561 tgccgcgctc cgtcgaccag gtggtggccg tgctcgccgt tgccaaggcc ggggccgcct
     1621 acctgccgat cgatccggcg tatccggccg aacgcatcgc gtacgtcgtc ggtgacgcgc
     1681 ggcccgtact gctcctggtg gacgcggtga ccggccgccg gctgccggag ctcgacaccg
     1741 gcacgcccgc gctggtcctc ggcggcgaca tcgccctgcg ccggccgggc caccgtccgg
     1801 cggcggacga gcggcgcgca cagctgacgc cggacagtcc cgcgtacgtc annnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 tctacacctc cggttccacc ggccgtccga agggcgtcgt ggtcacgcac cgcggcgtcg
     2101 ccaacctcgc cggaagccag atcgagcggt tcgcggtcga ggagggcagc cggatcctcc
     2161 aggtcgcctc gccgagcttc gacgcggcct tctccgagct gtgcctcgcg ctgctgtccg
     2221 gctccacgct ggtcctggcg ccggcggacc ggctgctgcc gggagccggg cggtccggtc
     2281 tggccggggt gatcgcacgg cagcgcatca cgcacgcgac gatctcgccc gccgcgctcg
     2341 ccgccctgga cgacgacggg ctgcccgccg gcatggtgct gaccgtggcc ggtgaggcgt
     2401 gctccgccgc gctggtcgac cggtggtcgc gcgaccgcag gatgatcaac gcgtacgggc
     2461 cgaccgagac caccgtctgc gccaccatga gcgagccgct ggccccgtcg gaccgccgta
     2521 cgccgccgcc gatcggccgt gccgtgtgga acacccacgc ctatgtgctg gacgcctcgc
     2581 tcggtccggt cgttcccggt gtcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnntccggt cgttcccggt gtcgccggtg agctgtacgt gtccggtccc
     2941 ggcctggccc gtggctatct gggccgtccg gatctgacgg cttcgcggtt cgtggcggat
     3001 ccgttcgcgg cggacggttc gcggatgtac cg
//
