LOCUS       NZ_KL573588             5035 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces baarnensis strain NRRL B-2842 P144_Doro1_scaffold61,
            whole genome shotgun sequence.
ACCESSION   NZ_KL573588
VERSION     NZ_KL573588.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces baarnensis
  ORGANISM  Streptomyces baarnensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Doroghazi,J.R., Albright,J.C., Goering,A.W., Ju,K.-S., Haines,R.R.,
            Tchalukov,K.A., Labeda,D.P., Kelleher,N.L. and Metcalf,W.W.
  TITLE     A Roadmap for Natural Product Discovery Based on Large-Scale
            Genomics and Metabolomics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Doroghazi,J.R., Ju,K.-S. and Metcalf,W.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2014) Institute for Genomic Biology, University of
            Illinois at Urbana-Champaign, 3109 IGB, MC-195, Urbana, IL 61801,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: IDBA UD v. 1.0.9
            Genome Coverage                   :: 74x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 02:19:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,395
            CDSs (total)                      :: 7,345
            Genes (coding)                    :: 7,173
            CDSs (with protein)               :: 7,173
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 55 of 173
            Pseudo Genes (incomplete)         :: 138 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 27 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5035
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KL573588.1.
            Bacteria available from NRRL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5035
                     /collection_date="2010"
                     /culture_collection="NRRL:B-2842"
                     /db_xref="taxon:66872"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces baarnensis"
                     /strain="NRRL B-2842"
                     /submitter_seqid="P144_Doro1_scaffold61"
     region          1..5035
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..5035
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5035
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1059:>2653](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1060..>2653
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(45..827)
                     /locus_tag="BG84_RS0137035"
     CDS             complement(45..827)
                     /NRPS_PKS="Domain: Thioesterase (20-235). E-value: 5.9e-52.
                     Score: 169.3. Matches aSDomain:
                     nrpspksdomains_BG84_RS0137035_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004: thioesterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143417.1"
                     /locus_tag="BG84_RS0137035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_050495843.1"
                     /sec_met_domain="PF00561 (E-value: 1.4e-08, bitscore: 22.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Thioesterase (E-value: 1.5e-53, bitscore:
                     170.0, seeds: 16, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1e-12, bitscore:
                     36.9, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEGSDVWFRRFRRVARPRLRLVCLPHAGGSASTFRSWPQWLPED
                     VEVLAVNLPGRQDRLGEDCVSDMDRLADGVAEALLPLSGEPFALFGHSMGASVAFEVAH
                     RLEQRYGTVAAALIVSGQQPPARYRVNEAHLGGDPGLLAEVRRLGGEGAAFLDSPEMRE
                     LLMPAIRGDFALMGAYRPDPSLRIAAPVDAWVGDADVDVTVEETRAWAEVTTGPFAYRV
                     FSGDHFYLNAQSAEFTGALLERLARPEAKAGSAPHQVR"
     aSDomain        complement(123..767)
                     /ASF="active site serine inconclusive"
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BG84_RS0137035_Thioesterase.1"
                     /evalue="5.90E-52"
                     /label="BG84_RS0137035_Thioesterase.1"
                     /locus_tag="BG84_RS0137035"
                     /protein_end="235"
                     /protein_start="20"
                     /score="169.3"
                     /tool="antismash"
                     /translation="RLVCLPHAGGSASTFRSWPQWLPEDVEVLAVNLPGRQDRLGEDCV
                     SDMDRLADGVAEALLPLSGEPFALFGHSMGASVAFEVAHRLEQRYGTVAAALIVSGQQP
                     PARYRVNEAHLGGDPGLLAEVRRLGGEGAAFLDSPEMRELLMPAIRGDFALMGAYRPDP
                     SLRIAAPVDAWVGDADVDVTVEETRAWAEVTTGPFAYRVFSGDHFYLNAQSA"
     CDS_motif       complement(510..572)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137035_0001"
                     /evalue="1.30E-08"
                     /label="NRPS-te1"
                     /locus_tag="BG84_RS0137035"
                     /protein_end="106"
                     /protein_start="85"
                     /score="27.2"
                     /tool="antismash"
                     /translation="PFALFGHSMGASVAFEVAHRL"
     gene            1060..>2653
                     /locus_tag="BG84_RS0137040"
     CDS             1060..>2653
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_Starter (8-300). E-value:
                     9.2e-104. Score: 338.9. Matches aSDomain:
                     nrpspksdomains_BG84_RS0137040_Condensation_Starter.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="BG84_RS0137040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_032791330.1"
                     /sec_met_domain="Condensation (E-value: 5.9e-48, bitscore:
                     151.1, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 8.8e-13, bitscore:
                     35.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDVQDLLLPLTAAQSGIWFAQELDPANVIYNAGEYLEIRGRVDA
                     AVFEAALLRVLAEAECLRVRFTDTSDGPRQRIAPVGDWRLPVLDVSAEPDPRAAAELRM
                     RDLLATPKDIRRDPLFDFVLFRLADDHHLWLHAYHHSAVDGFTVSLVARRTAEVYTALA
                     AGDEPTPTPFVPLTALIDADTAYRASDRFAADRAYWTDRMAGAPEPAGLSGGRPATPHR
                     LERRTAHLDRTSADALRDLARAAEVPWPPLLAAAFAAYLQRCTGGDEIVLGLPVTTRLG
                     RTARTAPGMVSNVLPLRLAPHDDMTVGELLRHTSAAMRGDLRHQRYRYEDLRRDLNLLA
                     DDQRLVGPQVNIMMFDYDLTFGGHRAVVHNLSVGPADDMSVVVYDRTDGNGLQIDLDAN
                     PELYTPDAIDAHLHRFLRYLRELAAAGPDQVLGTLGLVDEDERRLLLDTWNDTGHEVPA
                     VTLTELLTAQAAATPDAVAVLFEDTAVSYAELDDRAARLARLLVGRGVGPERFVAVAVP
                     RSVELVVALVAVLK"
     CDS_motif       1084..1125
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137040_0001"
                     /evalue="6.50E-05"
                     /label="C1_starter_004-017"
                     /locus_tag="BG84_RS0137040"
                     /protein_end="22"
                     /protein_start="8"
                     /score="15.3"
                     /tool="antismash"
                     /translation="LPLTAAQSGIWFAQ"
     aSDomain        1084..1959
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BG84_RS0137040_Condensation_Star
                     ter.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="9.20E-104"
                     /label="BG84_RS0137040_Condensation_Starter.1"
                     /locus_tag="BG84_RS0137040"
                     /protein_end="300"
                     /protein_start="8"
                     /score="338.9"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHSAVDG"
                     /tool="antismash"
                     /translation="LPLTAAQSGIWFAQELDPANVIYNAGEYLEIRGRVDAAVFEAALL
                     RVLAEAECLRVRFTDTSDGPRQRIAPVGDWRLPVLDVSAEPDPRAAAELRMRDLLATPK
                     DIRRDPLFDFVLFRLADDHHLWLHAYHHSAVDGFTVSLVARRTAEVYTALAAGDEPTPT
                     PFVPLTALIDADTAYRASDRFAADRAYWTDRMAGAPEPAGLSGGRPATPHRLERRTAHL
                     DRTSADALRDLARAAEVPWPPLLAAAFAAYLQRCTGGDEIVLGLPVTTRLGRTARTAPG
                     MVSNVLPLRLA"
     CDS_motif       1147..1263
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137040_0002"
                     /evalue="1.40E-16"
                     /label="C2_starter_024-063"
                     /locus_tag="BG84_RS0137040"
                     /protein_end="68"
                     /protein_start="29"
                     /score="53.0"
                     /tool="antismash"
                     /translation="IYNAGEYLEIRGRVDAAVFEAALLRVLAEAECLRVRFTD"
     CDS_motif       1471..1536
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137040_0003"
                     /evalue="2.10E-08"
                     /label="C3_starter_134-155"
                     /locus_tag="BG84_RS0137040"
                     /protein_end="159"
                     /protein_start="137"
                     /score="27.0"
                     /tool="antismash"
                     /translation="YHHSAVDGFTVSLVARRTAEVY"
     CDS_motif       1864..1953
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137040_0004"
                     /evalue="5.70E-14"
                     /label="C5_starter_265-294"
                     /locus_tag="BG84_RS0137040"
                     /protein_end="298"
                     /protein_start="268"
                     /score="44.3"
                     /tool="antismash"
                     /translation="EIVLGLPVTTRLGRTARTAPGMVSNVLPLR"
     CDS_motif       2002..2115
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137040_0005"
                     /evalue="1.60E-18"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="BG84_RS0137040"
                     /protein_end="352"
                     /protein_start="314"
                     /score="59.1"
                     /tool="antismash"
                     /translation="SAAMRGDLRHQRYRYEDLRRDLNLLADDQRLVGPQVNI"
     assembly_gap    2654..2883
                     /estimated_length=230
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2993..3313
                     /estimated_length=321
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3454..3763
                     /estimated_length=310
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3764..>4614
                     /locus_tag="BG84_RS0137055"
     CDS             3764..>4614
                     /NRPS_PKS="Domain: AMP-binding (1-266). E-value: 6.3e-65.
                     Score: 211.3. Matches aSDomain:
                     nrpspksdomains_BG84_RS0137055_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010268955.1"
                     /locus_tag="BG84_RS0137055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AMP-binding protein"
                     /protein_id="WP_030089431.1"
                     /sec_met_domain="AMP-binding (E-value: 3.3e-80, bitscore:
                     257.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGAVVPHAGIVNRLLWMQDHYRLTAEDRVLQKTPSGFDVSVWEF
                     FWPLITGAALVVARPDGHKDPLYLAELIQARSVTTTHFVPSMLQAFLAEPAAAGCTGLR
                     RVICSGEALPAPLRDRFHTVLTGDGPGPGPELHNLYGPTEASVDVTWWQCGPDDKTPSV
                     PIGRPVWNTRLYVLDARLRPVPAGAPGELYLAGVQLARGYLHRPGLTASRFVADPFAGD
                     GGGEGGGDGSGGGARMYRTGDVVRWTAGGDLEYLGRADDQVKLRGFRIELGEIEAAVLA
                     NP"
     aSDomain        3767..4561
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BG84_RS0137055_AMP-binding.1"
                     /evalue="6.30E-65"
                     /label="BG84_RS0137055_AMP-binding.1"
                     /locus_tag="BG84_RS0137055"
                     /protein_end="266"
                     /protein_start="1"
                     /score="211.3"
                     /specificity="substrate consensus: Ser"
                     /tool="antismash"
                     /translation="KGAVVPHAGIVNRLLWMQDHYRLTAEDRVLQKTPSGFDVSVWEFF
                     WPLITGAALVVARPDGHKDPLYLAELIQARSVTTTHFVPSMLQAFLAEPAAAGCTGLRR
                     VICSGEALPAPLRDRFHTVLTGDGPGPGPELHNLYGPTEASVDVTWWQCGPDDKTPSVP
                     IGRPVWNTRLYVLDARLRPVPAGAPGELYLAGVQLARGYLHRPGLTASRFVADPFAGDG
                     GGEGGGDGSGGGARMYRTGDVVRWTAGGDLEYLGRADDQVKLR"
     CDS_motif       4316..4405
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137055_0001"
                     /evalue="5.00E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="BG84_RS0137055"
                     /protein_end="214"
                     /protein_start="184"
                     /score="54.0"
                     /tool="antismash"
                     /translation="PAGAPGELYLAGVQLARGYLHRPGLTASRF"
     CDS_motif       4526..4591
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0137055_0002"
                     /evalue="1.10E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="BG84_RS0137055"
                     /protein_end="276"
                     /protein_start="254"
                     /score="42.8"
                     /tool="antismash"
                     /translation="YLGRADDQVKLRGFRIELGEIE"
     assembly_gap    4615..4888
                     /estimated_length=274
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
ORIGIN
        1 gcccggaacc tctccggttc cgggccgcct cgtcgtggtc ctgctcagcg cacctggtgt
       61 ggcgcgctgc ccgccttcgc ctccggccgc gcgagccgct ccaggagcgc accggtgaac
      121 tccgcgctct gcgcgttcag atagaagtgg tcgccggaga acacgcggta cgcgaacggc
      181 cccgtcgtca cctccgccca ggcccgcgtc tcctcgacgg tgacgtccac gtccgcgtcg
      241 ccgacccagg cgtccaccgg cgccgcgatc cgcagcgagg gatccggccg gtacgcgccc
      301 atcagcgcga agtcgccgcg gatcgccggc atcagcagct cacgcatctc cggactgtcc
      361 aggaacgccg cgccttcgcc gccgagccgc cgcacctccg ccaacaggcc cggatcgccg
      421 ccgagatgcg cctcgttgac gcggtaacgg gcgggcggct gctggccgga gacgatcagc
      481 gccgcggcca cggttccgta ccgctgctcc agccggtgcg ccacctcgaa ggccaccgac
      541 gcgcccatgc tgtggccgaa cagcgcgaac ggctcgccgg acagcggcag cagcgcctcc
      601 gcgaccccgt cggccagccg gtccatgtcg ctcacacagt cctcgccgag ccggtcctgc
      661 cgccccggaa ggttgacggc aagcacctcc acgtcctcgg gaagccactg cggccaggac
      721 cggaacgtcg acgcggatcc tcccgcgtgc ggcagacaga ccagccgcag ccggggccgc
      781 gccactcgtc tgaagcgccg gaaccacaca tcggacccct cggccatcac tccacctccg
      841 tcgtcgtgcc accgccgttc ggcgacgcca ccagtgcacc cgcaccaccg ctgccaccgg
      901 gccggtctgc aacaacgtgc acgcgcacgg gaatcggccg cgtaccgggc caggatgagc
      961 ggacgcgcct cctgtgcgtt ccgccgtgat gactgccggc agcggcccgt accgctcccc
     1021 ggccccggtg ttcccgaaaa cgaccgaggg tggttcccgt tgtccgacgt ccaggacctt
     1081 ctcctgccgc tgaccgccgc gcagtccggg atctggttcg cgcaggaact cgacccggcc
     1141 aacgtcatct acaacgccgg cgagtacctg gagatccgcg gccgggtgga cgcggcggtc
     1201 ttcgaggccg cgctgctgcg cgtgctggcc gaggcggagt gcctgcgcgt gcggttcacg
     1261 gacacctccg acggcccccg gcagcggatc gccccggtcg gcgactggcg ccttccggtc
     1321 ctggacgtct cggcggagcc ggatccgcgc gcggcggcgg aactgcggat gcgggacctg
     1381 ctggccacac cgaaggacat ccgccgcgat ccgctcttcg acttcgtcct gttccggctg
     1441 gcggacgacc accacctgtg gctgcatgcc taccaccaca gcgccgtcga cggcttcacc
     1501 gtctcgctgg tggcccggcg gaccgccgag gtctacaccg cgctggcggc gggggacgag
     1561 ccgacgccga cgcccttcgt cccgctcacg gcgctgatcg acgccgacac cgcgtaccgc
     1621 gcctccgacc gcttcgccgc cgaccgcgcg tactggacgg accggatggc cggcgcaccg
     1681 gagcccgccg gcctctccgg cggccgcccc gccaccccgc accgcctcga acgccgcacc
     1741 gcgcacctcg accggacgtc ggccgacgca ctgcgcgacc tggcccgcgc cgccgaggtg
     1801 ccgtggccgc cgctgctggc cgccgccttc gccgcgtatc tgcagcgctg caccggcggc
     1861 gacgagatcg tcctcggcct gccggtcacc accaggctcg gccgcaccgc gcgcaccgcc
     1921 cccggcatgg tctccaacgt cctgccgctg cgcctcgcac cgcacgacga catgaccgtc
     1981 ggcgagctgc tgcgccacac ctccgccgcc atgcgcggcg acctgcggca ccagcgctac
     2041 cggtacgagg acctgcgccg cgacctgaac ctcctcgccg acgaccagcg gctggtcgga
     2101 ccgcaggtca acatcatgat gttcgactac gacctgacct tcggcgggca ccgcgccgtc
     2161 gtccacaacc tgtccgtcgg accggccgac gacatgtccg tggtcgtcta cgaccggacc
     2221 gacggcaacg gtctgcagat cgacctcgac gccaacccgg agctctacac accggacgcg
     2281 atcgacgcgc acctccaccg cttcctgcgc tacctccgcg agctggccgc cgcaggaccc
     2341 gaccaggtcc tcggcacgct cggcctggtc gacgaggacg agcggcgact gctgctggac
     2401 acctggaacg acaccggcca cgaggtaccg gccgtcacgc tcaccgagct gctgaccgcg
     2461 caggccgccg ccaccccgga cgccgtcgcc gtcctcttcg aggacaccgc cgtctcctac
     2521 gccgaactgg acgaccgcgc ggcgcggctg gcccgcctgc tcgtgggccg gggcgtcggc
     2581 ccggagcgct tcgtcgcggt cgccgtgccg cgttccgtcg aactcgtcgt ggccctggtg
     2641 gcggtgctga aggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnggtgctg aaggccgggg ccgcgtatct gccggtggat cccggctatc cggcggaccg
     2941 gatcgcgttc ctgctggagg acgcggctcc ggtgctgacg ctgaccacct ccnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnccgccgt ggccgccgac ctgccggaga ccggcacccc gcgcctggtc
     3361 ctcgacaccg acgacaccgc cgcgctgctg gccgcccacg gcggcccgga ccacaccgca
     3421 ccgcttccgg ctccgctgcc cgcccacccg gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgaagg gcgcggtcgt
     3781 cccgcacgcc ggcatcgtca accggctcct gtggatgcag gaccactacc ggctcacggc
     3841 agaggaccgg gtgctccaga agacgccgtc cggattcgac gtctccgtct gggagttctt
     3901 ctggccgctg atcaccggag cggcgctcgt cgtcgcgcgc ccggacggac acaaggaccc
     3961 gctgtacctc gccgagctga tccaggcccg gtccgtcacc accacgcact tcgtaccgtc
     4021 catgctccag gcgttcctcg ccgagcccgc cgccgccggc tgcaccggcc tgcgccgcgt
     4081 gatctgcagc ggcgaggcac tgccggcccc gctgcgcgac cgcttccaca ccgtgctcac
     4141 cggcgacggt cccggccccg gcccggaact ccacaacctg tacgggccga ccgaggcgtc
     4201 cgtcgacgtg acctggtggc agtgcggacc cgacgacaag accccgtccg tgccgatcgg
     4261 ccgcccggtg tggaacaccc ggctgtacgt cctggacgcg cggctgcgcc cggtaccggc
     4321 cggagcaccg ggagagctct atctcgcggg cgtgcagctg gcccgtggct acctgcaccg
     4381 tccgggcctg acggcttcgc ggttcgtggc ggatccgttc gccggtgacg gcggtggtga
     4441 gggcggtggt gacggcagtg gtggcggtgc gcggatgtac cggaccggtg acgtggtgcg
     4501 gtggacggcg ggtggcgacc tggagtacct gggccgcgcc gacgaccagg tgaagctgcg
     4561 cggcttccgg atcgagctcg gcgagatcga ggcggcggtc ctggcgaacc cggannnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnag gcggccgtga tcgtccgcga ggaccgcccg
     4921 ggatcaccgg cggtggtggc gtatgtggtg ccggaggccg gtggtgtcgg tggtgtcggt
     4981 ggtgcgggcg gagcggtgga cgtgtccgtg ctgcgcggcc gggtggccgg tgtgc
//
