LOCUS       NZ_KL573582             7789 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces baarnensis strain NRRL B-2842 P144_Doro1_scaffold49,
            whole genome shotgun sequence.
ACCESSION   NZ_KL573582
VERSION     NZ_KL573582.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces baarnensis
  ORGANISM  Streptomyces baarnensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 21173)
  AUTHORS   Doroghazi,J.R., Albright,J.C., Goering,A.W., Ju,K.-S., Haines,R.R.,
            Tchalukov,K.A., Labeda,D.P., Kelleher,N.L. and Metcalf,W.W.
  TITLE     A Roadmap for Natural Product Discovery Based on Large-Scale
            Genomics and Metabolomics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21173)
  AUTHORS   Doroghazi,J.R., Ju,K.-S. and Metcalf,W.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2014) Institute for Genomic Biology, University of
            Illinois at Urbana-Champaign, 3109 IGB, MC-195, Urbana, IL 61801,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: IDBA UD v. 1.0.9
            Genome Coverage                   :: 74x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 02:19:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,395
            CDSs (total)                      :: 7,345
            Genes (coding)                    :: 7,173
            CDSs (with protein)               :: 7,173
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 55 of 173
            Pseudo Genes (incomplete)         :: 138 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 27 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 7789
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KL573582.1.
            Bacteria available from NRRL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..445
                     /locus_tag="BG84_RS0136650"
     region          1..7789
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..7789
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7789
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2324:2789](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(2325..2789)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     CDS             <3..445
                     /codon_start=2
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956275.1"
                     /locus_tag="BG84_RS0136650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="WP_030089302.1"
                     /transl_table=11
                     /translation="LARLGTQLQARMDRWVREAGSPPQPRSTITAILSSVLPTDEESRR
                     ITRIYAAYYVLVLGDSSLEEHATPQPALLEGFLAKQLRLAQEAGTVGPEKDPAMVAAGL
                     MAMVNGLGSSVLGGQRTGKAALLVLRHHLDELFGPATPASDGS"
     gene            727..1113
                     /locus_tag="BG84_RS1000000139825"
     CDS             727..1113
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BG84_RS1000000139825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158688347.1"
                     /transl_table=11
                     /translation="MTNWTNSGSLSDLLTISDVPLQPDPPQKGGALKVTISGRLNTLPG
                     EGATGRVLVKYGFITLSNTTPKWSELLAGEPDPTEPGPVSLVFPVSLDAQRTERQVHAH
                     RLRDRRGRPPGRLRRPRSRPVGRR"
     gene            complement(2325..2789)
                     /locus_tag="BG84_RS1000000140705"
     CDS             complement(2325..2789)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.4"
                     /locus_tag="BG84_RS1000000140705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_050495832.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.4e-11,
                     bitscore: 35.4, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTLGKLALGAVSMLTAAGSLLVAGPAVAAAPAAEVAPQVQVFTA
                     AAGVAPPAQLIGPNGEKPTEWGVASFDMLRSGSPVAKASVGGGTWLSGTTAALTGKTCY
                     SNYIHPTKKHSASIAFAGGTKKDIRRKDVWAKAKITAGAAYTCNTYWGVY"
     gene            3059..3580
                     /locus_tag="BG84_RS1000000140910"
     CDS             3059..3580
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /locus_tag="BG84_RS1000000140910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_324605530.1"
                     /transl_table=11
                     /translation="MHQALFAQFCGANVPGPSDAAAARARSTAVLEGVTAQGATSSAIA
                     SDLRVSGHSVQRWRRMWDEGGPRALRSRGPASLPRLSEKQFARLRPREISAPPLQTPRR
                     PMETPHGTPRRPSSPSPAASSAGGASRTVLWALNASYGLLREHWLPGFRAARDGVRVNA
                     LHWRSGTQCS"
     misc_feature    3458..3460
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3685..>4110
                     /locus_tag="BG84_RS0136665"
     CDS             3685..>4110
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982141.1"
                     /locus_tag="BG84_RS0136665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="WP_158688348.1"
                     /transl_table=11
                     /translation="MLGLGAVGVAAAPMLQGGLERFLGAAARKDPTGLTGLLPNGGGFR
                     YYSVVSSVPEKTAATFRLTVDGLVDRPGSYTLDELRALPQTRVVRDVQCVTGWRVPQTA
                     FEGVTLSRILDAAGVQARGRAIRFTCFDGAYSESLTLD"
     assembly_gap    4111..4356
                     /estimated_length=246
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4357..4604
                     /locus_tag="BG84_RS0136670"
     CDS             <4361..4604
                     /codon_start=3
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576522.1"
                     /locus_tag="BG84_RS0136670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="WP_030089308.1"
                     /transl_table=11
                     /translation="LDQARREDVMVALRMRDEPLSHTHGGPARLYVAPMYFYKSAKWLS
                     GITVTPEVVPGYWEERGYDVDAWVGRSNGRDDAPTV"
     gene            4588..5280
                     /locus_tag="BG84_RS0136675"
     CDS             4588..5280
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933355.1"
                     /locus_tag="BG84_RS0136675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="WP_078611331.1"
                     /transl_table=11
                     /translation="MPPPSEPTTRVPRFTAAQRWVHRTTAWLTLTCVATAACLYVPQLA
                     ELVGRRHLVRDVHIWSGLLVPAPLLLGLASRALRADLGRLNRFGPHDRRWLRAALRRDR
                     RAASRPADKFNAGQKTYAAWIAGAVLVLLGTGLMMWFTGLTPLVWRTAATFVHDWLALA
                     VGIVLAGHIGKALADPESRRGMRTGTVDPVWAAVEHPLWHPDEARPHALHSHGRGRDAD
                     APFDAGSR"
     gene            complement(<5471..>6578)
                     /locus_tag="BG84_RS01000000137880"
                     /pseudo=""
     CDS             complement(<5471..6578)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611324.1"
                     /locus_tag="BG84_RS01000000137880"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="ISAzo13 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDRDAQFRYVNEQARGHMGAGQSVISVDMKKKELVGDCKNAGRQW
                     RLAGEPALAKTHDFLDRQGPGKAVLYGIYDIAANTGRVCVGTNHDTAAFAVASIRRWWK
                     ARGRHDYPVATRVLITADAGGSNGYRTRAWKT"
     misc_feature    complement(6551..6567)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.6"
                     /tool="antismash"
     gene            complement(6886..7665)
                     /locus_tag="BG84_RS0136690"
     CDS             complement(6886..7665)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /locus_tag="BG84_RS0136690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thioredoxin domain-containing protein"
                     /protein_id="WP_030089314.1"
                     /transl_table=11
                     /translation="MSKSSIRAQSRKARERLRAERERAAARSRRRRRAAISAGVAAAII
                     SAVGLTVVVQSSRAADNRPVVPPAGAVGADKLVISVGQADAPATLTLYEDPRCPGCAQV
                     ERLLHTTINRLQDEGKLRVDYHVLSFVDNIVSGTGSKHAANALAAAQNAGRFREYHDAL
                     FAAQPKSETDDAFGDKQVLLDLAAKVKGLDTDRFAAAVKDGTHDTWVNKVQEEFDKQTE
                     IQGTPAVFFKGKDLIRDTEHPLTPERLAKLVEAEAEH"
ORIGIN
        1 cctagcccgg ctgggcaccc agctccaggc ccgcatggac cggtgggtcc gcgaggcggg
       61 ctctccgccg caaccacgct ccacaatcac cgccatcctc tccagcgtcc tgccgacgga
      121 cgaggagagc cgccggatca cccggatcta cgccgcctac tacgtgctcg tcctcggtga
      181 cagctcgctg gaagaacacg ccacacccca acccgcacta ctggaaggct tcctcgccaa
      241 gcagctccgc ctggcgcaag aggccggcac tgtcggcccc gagaaagatc ccgccatggt
      301 ggcggccgga ctgatggcca tggtcaacgg cctcggctcc agcgtgctgg gcggccagcg
      361 caccggtaag gcagccctgc ttgtcctgcg ccaccacctg gacgaactgt tcggccccgc
      421 tactcctgca tccgacggct cgtgacgaca taccacccga cgctgaatcc accacgcaag
      481 atcatttgaa gacaccgtct aaagatcaac ccccgggctt cgagccggcc gagctggtgg
      541 gccaccgaac agtacgacga tgtggagtgg ccccggcggt tccgcggggc tcctgcgccc
      601 gaaggtccgc tcaccccccg aggagaccgc ccgcgcgagc gcctggtcgc tcagcgcgcc
      661 gaactcaccg ccgccgaggt ctttcccccg gccgtggagc agggcctgtc aacgaccgtt
      721 tcgggcatga ctaattggac gaacagcggc tccctcagtg acctgttgac catcagcgac
      781 gtgcccctgc agccggaccc gccgcagaag ggcggcgcgt tgaaggtcac catcagcggt
      841 cgcctgaaca ccctgccggg tgaaggcgcg acgggccgcg tgctggtcaa gtacggattc
      901 atcaccctgt cgaacacgac acccaagtgg tccgagctgc tcgcgggcga gcccgacccg
      961 accgagcccg gccccgtcag cctcgtcttc cccgtctcgc tcgacgccca acgcaccgag
     1021 cggcaagtac acgctcaccg cctccgtgac cgccgcggac ggccgccagg tcgcctccgt
     1081 cgccctcgaa gtcggcctgt aggacgccgc tgacgcacaa gccgtcactc tcggcgggtt
     1141 ccgcctggcc atggccggcc gcgccaggcg tgctccattg ctcagcgctc tggagcatcg
     1201 gatcccgacg gacgcgcagc ggcgcgcccg cgcggccatg ctcaccacgc actgcaccac
     1261 cagcaccgcg gttgcggcct acgcccacca cctgccgctc gtacacttcc agctctgctg
     1321 gggaaggggc ggtgttccga ccgtgcgcag ttcctggcgg agttggcgtc tgggtgaact
     1381 gaacggcgac actggcgagc aacgattttc tgatcatagc gaaacgcgat tcggggtatt
     1441 ttgcttgcgg caactcgcag acaagggcaa ctctaaaagc gagactggct catcccagcc
     1501 gaacaacagc tgggatgagc cagtctcgat cagcaggcgg ccttgctcaa gaggcgacat
     1561 ttactggcat taggctcgcg gaggttgccg acgccaggtc tccacgaccg cgacgcacaa
     1621 gagtgccagc atcacggtca cagccagagc gcgggttacg ctccaagtcg cacccaccaa
     1681 cggtccttgc agatgggcct caacggacag ccgcatagtg gtttcaccga taaccacaac
     1741 tgggattaga agccgggaag cgagaccgca gggtccgact cgcgcgagat gtccggcgat
     1801 tccgaagatc gggcccgcag cgagagcaaa ggcccaccac agaacgagga tcgataggaa
     1861 atcgccccac gcgaagactg tagctttcgc cgtcgggtca ctgtagttcg gtgccacgaa
     1921 ttcaccttga gtcgctttcg tcagatagta ggcaaacacg gccgctatca gcgaaatcga
     1981 tcccataatg gccgcgcgct tttgggagcc ggccaagaac ccggcaataa aggcgactag
     2041 cgcgtacatc caacctgcga caagagtgat gcttgccgca tggctcacgc gcccgcttac
     2101 tgattcgagg agtggaccag agatcccaac gatcaaaccg ccaacaatgg gaaatgtggc
     2161 ggctgagaga tatgggcgca cgtgcacaac cttgtcgcgt gggggaatgg atccggctcc
     2221 ctaccgcatc acgagcggaa attgggcagg caaagctttt accgcctcat cgtgaaaacg
     2281 agtggccgag cattcgacca gcggaggcgc gggcgggcta agctctaata gacaccccaa
     2341 taggtgttgc aggtgtatgc agcaccagcg gtgatcttgg ccttggccca gacgtccttc
     2401 ctccgaatgt cctttttggt accgccagcg aaagcgatgc tggcagagtg cttcttggtc
     2461 ggatggatgt aattcgagta gcacgtctta cccgtcaggg cggccgtggt gccagagagc
     2521 caggttccgc caccgacgct ggccttcgcc accgggctgc cgctgcgcaa catgtcgaag
     2581 gacgctacgc cccactcagt gggcttctcg ccattggggc ctatgagctg ggccggcggg
     2641 gcaacgccag cagccgccgt gaagacctgg acctgcgggg cgacctcggc ggccggagcg
     2701 gccgcgacgg ccggcccagc aaccaggagg gatccggcag cggtgagcat agacaccgcg
     2761 ccgagagcga gctttccaag cgtcttcatc aaaatctcgt ttcatgtgtg aagatcatgc
     2821 atttcctctt gctcgcgttg gcttgaggtg caagacgaaa agggctcgag agtagctgtg
     2881 ccaggcccgg aagaagccgg cgcagggtcc ccatcagggc gcggtggaac cgcgttggct
     2941 ggggtggcct ttcgccctcc gattctcacc tcacgtaagg aaattcggca cctctatcac
     3001 ccctatgagc ccataactct ccgctctacc tctgagtaga ggcggagtgt gatctcacat
     3061 gcatcaggct ctcttcgcgc agttctgcgg cgcgaacgtc cccggaccgt ccgacgccgc
     3121 cgcggcccga gcccgatcga cagcggttct ggaaggagtt acggcccaag gtgcgacaag
     3181 ctcggccatc gccagtgacc tgcgggtcag tggccactcg gtgcagcggt ggcggcggat
     3241 gtgggacgaa ggtggtccgc gggctctgcg gtcacggggg ccagcgtcac tgccgaggct
     3301 gagcgagaag cagttcgccc ggcttcgacc gcgagagata tcggctccac cacttcaaac
     3361 gcctcgccgt ccgatggaaa cgccgcacgg aactccacga cgcccttcgt ctccctcgcc
     3421 tgcagcctct tctgctggag gcgcctcaag aactgtgtta tgggctctaa acgcctctta
     3481 tggactccta agagagcact ggctaccggg ttttcgggct gccagggatg gtgtacgtgt
     3541 aaatgctctt cactggagat cggggacgca gtgcagctga agaagaggcc ggccttccgg
     3601 ccaccgcggg tgtcgaatcg aggcgtggat gaagaacgac cggaacagcg cggcgccccc
     3661 gtaggccgcc gtatcctcct cagcatgctc ggactcggcg ccgtcggggt ggccgccgcg
     3721 cccatgctcc aaggcggcct ggagagattc ctgggggccg cggcccggaa ggaccccacc
     3781 ggcctgaccg gcctgctccc caacggcggc ggattccggt actactccgt ggtctcgtcc
     3841 gtccccgaga agaccgccgc gaccttccgc ctcaccgtgg acggcctggt cgaccggccc
     3901 ggctcgtaca ccctcgacga gctgcgggcc ctgccgcaga cccgggtggt gcgcgacgtc
     3961 cagtgcgtca ccggctggcg cgtcccgcag accgcgttcg agggcgtcac gctctcccgc
     4021 atcctcgatg cggccggcgt ccaggcgagg ggccgggcca tccgtttcac ctgcttcgac
     4081 ggcgcctaca gcgagagcct caccctcgac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccct cgaccaggcc cgccgcgagg
     4381 acgtcatggt cgctctgcgg atgcgcgacg aacccctcag tcacacccat ggcggccccg
     4441 cccgcctcta cgtcgcaccc atgtacttct acaagtccgc gaaatggctc tccggcatca
     4501 ccgtcacccc cgaggtggtg cccggctact gggaggagcg cggctacgac gtcgacgcct
     4561 gggtcggccg ctcgaacgga cgtgacgatg cccccaccgt ctgaacccac cacccgtgtc
     4621 ccgcggttca ccgccgccca gcgctgggtg caccgcacca cggcctggct caccctgacc
     4681 tgcgtcgcca ccgcggcctg cctgtacgtt ccccagctcg ccgaactcgt cggccgccgc
     4741 cacctggtca gagacgtcca catctggtcg gggctcctcg tcccggcgcc gctgctgctc
     4801 ggcctcgcct cccgcgcact gcgtgccgac ctgggccggc tcaaccggtt cggcccgcac
     4861 gaccgccggt ggctgcgcgc cgccctgcgc cgtgaccgca gggccgcgtc ccggcccgcg
     4921 gacaagttca acgccgggca gaagacgtac gcggcctgga tcgccggcgc ggtcctggtc
     4981 ctgctcggca ccggcctgat gatgtggttc accggcctca ccccgctggt ctggcggacg
     5041 gcggcgacct tcgtgcacga ctggctcgcc ctcgcggtcg gcatcgtgct cgccgggcac
     5101 atcggcaagg ccctcgccga cccggagtcc cgccgcggca tgcgcacggg aacggtcgac
     5161 ccggtctggg cggccgtcga gcacccgctg tggcacccgg acgaggcccg cccgcacgca
     5221 ctccattccc acggccgcgg aagggatgcc gacgcccctt ttgacgccgg ctcccggtga
     5281 cccttgtagc cgatctgctc ggcgagcaac tggacctggg tgcggcccac cacccgctcc
     5341 ccttcccgcc gccaccccgg ccccgccctc ccgcggagcg atccccagtt cctccgcaat
     5401 cctcggatgg gcctccacgg ggtgcgacct gagcctgagt acccgggatc acctcgactg
     5461 cgatgcacgg gatggtgtag ccgtgcttgg ccaggaaggg ctggacttgg tgaacggccc
     5521 tggtgagggt gctggtgttt actccgacga gctgggcgag gaaatcgcgg gtgccgagtt
     5581 tacgcaggta gaacacggtg gccaggattc tgttggcggt ggtcagcttg tccttggcct
     5641 cagcgccccg agcgcggatg cgttcgcctc ctagctgccg gggccatcct ttctgacgta
     5701 cattgttcag attcgaggac aattggccga tgagttcgtc cagcgctggt ccggacatgc
     5761 cggtcagctc cggtttgcgc aggctcccgg tgcagggctg cagagagggg ccaccggcca
     5821 gcggtggggt cgtggtttgt gcttgtggcg tcgcaggtga agggtgtagt tccagtcgcc
     5881 gtggaaacgg tgccggtgca tgggcagagc gtcgatatcg ctatcggtga ccttgatgcc
     5941 ggtgtcgtag gtgcctgggt cgagttcggc gtggacggtc agcccgttgc gggttgtggt
     6001 cgccgcgata ctgttcacga tgacgtcgtg gctgatcagc gggcggccgc gccagttcat
     6061 ggagatgtgg gagaacagcc ggtgctcgat cttgttccac ttcaggtgcc cggcggcctg
     6121 tggcagaccg tgaagtccca acccgtctcg acggccaagg cggcgagtta ggtcttccag
     6181 gcgcgggtgc ggtagccgtt ggacccgcct gcgtccgcgg tgatcagtac acgggtggcg
     6241 accgggtagt cgtgccggcc ccgggccttc caccagcggc ggatggaggc gacggcgaac
     6301 gcggcggtgt cgtgattggt gccgacacag acccggccgg tgttcgcagc gatgtcgtag
     6361 atcccgtaga ggaccgcctt gcccggcccc tgccggtcaa ggaagtcgtg cgtcttggcc
     6421 agggccggtt caccggccag ccgccactgg cggcccgcgt tcttgcagtc gccgacgagt
     6481 tccttcttct tcatgtccac gctgatcacc gactggccag cgcccatgtg tcctctggcc
     6541 tgctcgttga cgtagcggaa ttgggcgtcg cggtccggat caagatctgt ggatctcttc
     6601 cggccaccac cgggcctgcg ctccggctcc ggttcggggg ccggttcggc ggctgcgggc
     6661 gcgagccggc tgctccccga ggggcgcatg agtgcttggc acctggagct cgccaacttc
     6721 caggagctgc tgctgcccgc ccccgtgcga ggcggggcaa atcggcccct ggaagctggc
     6781 catcgatctg ctgggtcagg tgtcccaaca gctgcccggc ttccggccgc gatccgcgca
     6841 cgggctaaag agccgttcac cctttggagt ggccgctgaa gaagttcagt gctccgcctc
     6901 cgcctccacc agcttcgcca atcgctctgg cgtcaggggg tgttcagtgt cccttatgag
     6961 atccttgccc ttgaagaaca ccgctggcgt gccctggatc tcggtctgct tgtcgaactc
     7021 ttcctggacc ttgttcaccc aggtgtcgtg ggttccgtcc ttgaccgcgg cggcgaagcg
     7081 atccgtgtcc agccctttga ccttcgctgc gagatccagc aggacctgct tgtcgccgaa
     7141 ggcatcatcg gtctcggact tcggctgagc cgcaaaaagg gcgtcgtggt actcgcggaa
     7201 cctgcccgcg ttctgtgctg ctgcaagagc gttagccgcg tgcttcgatc ccgtgccgga
     7261 gacgatgttg tcgacgaagg acaggacgtg gtagtcgact cggagcttgc cctcgtcctg
     7321 gagcctgttg atcgtcgtgt gcagcagtcg ttcgacctgt gcgcatcccg gacagcgggg
     7381 gtcctcgtac agcgtcaatg tggccggggc gtccgcctgg ccgacggata tgacgagttt
     7441 atcggctccc acggcacccg ccggagggac gactggcctg ttgtcggcgg cccggctcga
     7501 ctgcaccacg acggtcagcc ccacggcgga gatgatggcg gcggccacgc ccgcactgat
     7561 ggcagccctg cgcctgcgtc gggagcgggc ggccgcacgt tcccgctcgg ccctgaggcg
     7621 ttcgcgggcc ttgcgggact gtgcgcggat ggaggactta ctcatgggag acctacttcg
     7681 tgcgtgttcg tcgtgatgcg gtgcgaattc gcgatggggt acggaatctt cgcgggtact
     7741 gcggcccgcg ctgtcagagc cttgcgtcga gcgagaagcg gctcgcggg
//
