LOCUS       NZ_KL573576            10798 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces baarnensis strain NRRL B-2842 P144_Doro1_scaffold42,
            whole genome shotgun sequence.
ACCESSION   NZ_KL573576
VERSION     NZ_KL573576.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces baarnensis
  ORGANISM  Streptomyces baarnensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 69963)
  AUTHORS   Doroghazi,J.R., Albright,J.C., Goering,A.W., Ju,K.-S., Haines,R.R.,
            Tchalukov,K.A., Labeda,D.P., Kelleher,N.L. and Metcalf,W.W.
  TITLE     A Roadmap for Natural Product Discovery Based on Large-Scale
            Genomics and Metabolomics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 69963)
  AUTHORS   Doroghazi,J.R., Ju,K.-S. and Metcalf,W.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2014) Institute for Genomic Biology, University of
            Illinois at Urbana-Champaign, 3109 IGB, MC-195, Urbana, IL 61801,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: IDBA UD v. 1.0.9
            Genome Coverage                   :: 74x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 02:19:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,395
            CDSs (total)                      :: 7,345
            Genes (coding)                    :: 7,173
            CDSs (with protein)               :: 7,173
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 55 of 173
            Pseudo Genes (incomplete)         :: 138 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 27 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 59134
            Orig. end                         :: 69932
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KL573576.1.
            Bacteria available from NRRL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5798](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5798
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1050..1592
                     /locus_tag="BG84_RS0135995"
     CDS             1050..1592
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955650.1"
                     /locus_tag="BG84_RS0135995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5709 domain-containing protein"
                     /protein_id="WP_030089068.1"
                     /transl_table=11
                     /translation="MSDTDWGDDVYQPQEPEASDPDEQLGAEDTLMDPSGTGDPLDEGY
                     SPPDRPWVVDGLGTTADERNTGESLETRLTREVPEPDGSAGDGVGDLVDGDGEPWDDEV
                     GVARAGRLTQQADGRVPATLTASDVGIDGAAASAEEAAMHVIPEPGDDDALLEAGPPPA
                     APGSGPAGGGTAAPPPS"
     gene            1739..3178
                     /gene="gndA"
                     /locus_tag="BG84_RS0136000"
     CDS             1739..3178
                     /EC_number="1.1.1.44"
                     /GO_function="GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_process="GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="gndA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141148.1"
                     /locus_tag="BG84_RS0136000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="WP_030089070.1"
                     /transl_table=11
                     /translation="MSGTAQIGVTGLAVMGRNLARNFARNGFTVAVHNRTTSRTDALVE
                     EFGDEGTFVAAHTPEEFVAALERPRRLVIMVKAGDPTDAVIQEFAPLLEEGDVIIDGGN
                     AHFEDTRRREKELRERGIHFVGVGISGGEEGALNGPSIMPGGSAESYASLGPMLEKIAA
                     QAKDGTPCTTHIGPDGAGHFVKMVHNGIEYADMQLIAEAYDLLRAVAGYSPAQIADVFR
                     TWNTGRLDSYLIEITAEVLAHTDAATGKPFVDIVQDRAEQKGTGRWTVQIALDLGVPVS
                     GIAEAVFARSLSGHADLREAARGLPGPTPKPLDGAAAAAFADQVEQALYASKVVSYTQG
                     FHQIRAGGETYDWDIDLGSVAAIWRAGCIIRAAFLDRIRAAYDSRPDLVSLLSDKEFGQ
                     EIGAAQDDWRAVVAEAVRQGVPTPGFAAALSYYDGLRADRLPAALTQGQRDFFGAHTYR
                     RTDREGSFHTLWGGDRTEVES"
     gene            complement(3209..3625)
                     /locus_tag="BG84_RS01000000137830"
     CDS             complement(3209..3625)
                     /GO_function="GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /locus_tag="BG84_RS01000000137830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="WP_030089072.1"
                     /transl_table=11
                     /translation="MALTAPRRRTAIVFASLVLLGGKATGGVAAAAETGAAAAAAYPCD
                     VKMSAAGRLSAGYYDGTTVVPSTSQVTAAGKEAQCLLQYQGHNPGTIDGIFGRNSQAAA
                     KKFQKGVNYACRTTLAEDGKVGQQTWPYLRRLYC"
     gene            3895..5001
                     /locus_tag="BG84_RS0136010"
     CDS             3895..5001
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456253.1"
                     /locus_tag="BG84_RS0136010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_030089074.1"
                     /transl_table=11
                     /translation="MPDAVPDPVSVQPVVAPLTSAALFLVGTIEPGGEGAVRDVLPDLA
                     AVARSLGFRYPDSGLACVAGFGSSAWDRLFAGPRPSHLHPFPELRGPLHHAPATPGDVL
                     LHIRAERMDVCYAWAAQLLDKLGGALRIVDETHGFRYLDHRDLLGFVDGTENPVGDDAR
                     SAALVGADDDPDFEGGSYVVVQKYLHDLTSWNALTVEEQERVIGRTKLDDVEFPDEDKP
                     ADSHLALNTITDPDGTERDILRANMPFGSFGKGEFGTYFIGYAADPDVTERMLRNMFLG
                     DPPGTHDRILDFSTAVTGSLFFAPRADFLDAPPPLPAPARSGDRPEPVPVPVLQEPGPA
                     PASARQEPVAAGPDHGSLRIGSLQESAQ"
     gene            5001..5798
                     /locus_tag="BG84_RS0136015"
     CDS             5001..5798
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955648.1"
                     /locus_tag="BG84_RS0136015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_030089076.1"
                     /sec_met_domain="Linocin_M18 (E-value: 4.9e-90, bitscore:
                     293.2, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVTPSAWDEIEEEARRTFRRHVAGRRVVDVSDPDGP
                     TLSAVGDGHLRDIDPPTPDVVARARTSMPVIEWRVPFTVTRQAVDDVERGSADSDWQPV
                     KDAARTCAFAEDMAIIDGYGAAGITGLRDGSSHEPLPLPADARDYPVAVSQAVTRLRLA
                     GVDGPYRLLLGADAFTEAAETSDHGYPVKTHLSRLVDDEILWAPAVKGGVLLSTRGGDF
                     ELCLGQDLSIGYADHDATSIHLYFQQAFTFRMLTPEAVVGLIA"
     gene            5831..7207
                     /locus_tag="BG84_RS0136020"
     CDS             5831..7207
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456256.1"
                     /locus_tag="BG84_RS0136020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_030089078.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 1.6e-39, bitscore:
                     127.9, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 1.8e-09,
                     bitscore: 30.0, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQTTSAPHHEDGTAPPAARFDAVVEAAVQQGLLGEGCPVAGFID
                     TEGVRASVGTLHDAFAATPGVLHTFAAKACSLIPVLRLLADCGMGCEVASPGELRLALE
                     AGFPASRIVLDSPAKTREEIRLALALGVAVNADSLDELRRVDALRNPGTASVLGLRVNP
                     QVGGGAIGAMSTATDTSKFGVALRDAGAREAIVTAFRERPWLTRLHAHVGSQGCPLELI
                     AAGVAQTYELAEEINATLGVRQVTSLDIGGGLPVNFADETVHPTYADHVAALTAAAPGL
                     FSGHYGLVTEFGRSLLAKNGFIGARVEYTKDAGGRRIALTHAGAQTATRTVLMPDAWPL
                     RIGAFGPDGTPKGGPVLAQDIAGPCCFAGDVVAYGRELPELAQDDVVVLYDTGAYYFST
                     PWAYNSLPRPAVYGFRVAAGVGRDRAAVTFATVRDAQSMDAIAAESGLAHADALVGRSD
                     "
     gene            complement(7394..7600)
                     /locus_tag="BG84_RS1000000138960"
     CDS             complement(7394..7600)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BG84_RS1000000138960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_078611318.1"
                     /transl_table=11
                     /translation="MTHPYPDPVPPSPTPPPVPGPPTPVPGPPPVPPGPGPVPPGPAPD
                     PIPQPPGPDPVPDPEPEPQPAGT"
     gene            7815..8585
                     /locus_tag="BG84_RS0136025"
     CDS             7815..8585
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955646.1"
                     /locus_tag="BG84_RS0136025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PIG-L family deacetylase"
                     /protein_id="WP_030089080.1"
                     /sec_met_domain="PIG-L (E-value: 1.8e-23, bitscore: 75.9,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLPSILAVHAHPDDESLFCGGVLARHAAAGARTAVVTATWAPGT
                     HRAAELARALDALGAGAPRLLGYADARVPESAPGRPRFLDAPLDETVEAVVGHIRDVRP
                     EVVITGDAYGGMTGHPDHVHAHRITALAVRAAGLPQYGPHAGDPWQPSALYLATHPRSA
                     AVPVGGRLARAGTPAGALYCSEDAWITTTVDVGPWLPQKLAAILAHRSEVERGAAPGRI
                     AALPPAVQREVLGTEWYIRQDLTPRSGSATELSA"
     gene            complement(8613..8966)
                     /locus_tag="BG84_RS0136030"
     CDS             complement(8613..8966)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955645.1"
                     /locus_tag="BG84_RS0136030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_030089082.1"
                     /transl_table=11
                     /translation="MAENAAVEIRDDREHGNLVAYEDGTSAGTIAYFVMDPAPGALVAV
                     HTVVRPEHEGKGIAGALVREFYAMAAREGVPVVPLCPYAAKWAQRHPDEAPAAPGGLVR
                     EAERQLRHHPELF"
     gene            complement(9033..9458)
                     /gene="panD"
                     /locus_tag="BG84_RS0136035"
     CDS             complement(9033..9458)
                     /EC_number="4.1.1.11"
                     /GO_function="GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="panD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964661.1"
                     /locus_tag="BG84_RS0136035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aspartate 1-decarboxylase"
                     /protein_id="WP_030089084.1"
                     /transl_table=11
                     /translation="MLRTLFKSKIHRATVTQADLHYVGSVTIDAALMEAADLLPGELVH
                     IVDIDNGARLETYVIEGERGSGVIGINGAAAHLVHPGDLVILISYAQVDDAEARALVPR
                     VVHVDADNRIVALGDDASAPVPGSRTERSPQAVVPGG"
     misc_feature    9614..9633
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.72"
                     /tool="antismash"
     gene            complement(9956..10102)
                     /locus_tag="BG84_RS1000000138965"
     CDS             complement(9956..10102)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BG84_RS1000000138965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_234024708.1"
                     /transl_table=11
                     /translation="MDDDARECFNRTLRRDLEAGVWDERFGHLRTRPTCEGSLVVIRAT
                     LEA"
     gene            10199..10780
                     /locus_tag="BG84_RS0136045"
     CDS             10199..10780
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BG84_RS0136045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_234024709.1"
                     /transl_table=11
                     /translation="MLKSTDDADTSTDHSRLVINDTVYAPTAAVDLPMSRVSSQVVART
                     IDLGISPATGYLRPVIEIPPEPMLVTTYPAVIAKPASVTAVTGFTAPAPGAPVDVTNAT
                     IPGDDQASLTLGSYTRPAPATTGPLDHVVLQVAHHEDGDVGSVRLLVDFPSSTCPSVDS
                     LTLPVRGRPEGRPPPRGVWWPCMTPSALRP"
ORIGIN
        1 cgcgtcgtga acggcttcgc cgccccggtg gtgaccttct ccttcgacgg ctgggcgtag
       61 ctgccgtaga cccacaggcg ggcgttctcc cgggccgctt cggcggtgct tcgggcgtcc
      121 ggctcgcccc gggagcccgc ggaggccagc ggagtgtcct cctgggtgcc gttgccgtcg
      181 tcgtcggacc ggcaccactt ctcctggagg atcgccgggg ccgagaaccc gacgagcggc
      241 atctcctcgg ggtcgtcgtt ctcggcgacg taactcaccc agccggtcga cttgagcgcc
      301 cactggacgg gcacgtcgaa ggcgatgccg cgcttggggt tgaccacggt cttccagccg
      361 ggtacgaggg gcgcgggccc gttctcggca ccgccccggg cctcgcccgg ggacgccgac
      421 ggtgacacgg tgtccgtccc ggtgggtccc gcgaccggct tcccgtcgtc ctcgtcgccg
      481 ccgagcacca ggaatccggt gattccggcg gcgatgacga cggccgcggc cgcgaccacg
      541 gcgatgaccg agctacggcg gctgccgccg ggcgggggcg gcccggagga agcggtccgc
      601 gcgctccagg gggcggacgc gggctgcccg gcaggttcgg tgcgcgcgtc ccacggagcg
      661 gtcccggctc ccgcgtacgg cggctgcccg ccggcctgct gtccctgcgg aggccacccg
      721 tacggctgct gcccctggcc ctgtccgtac ggctgctgcg gctggccctg ctggtggtga
      781 tcgcccccgg gcgactgctg tccgttcccc atgggagatc accctatgcc cggccgttcg
      841 acgattcgaa cagcctgtgc ccggactccc acgaactcct cgcttccgcg gcgaaccggc
      901 cgcgacctgc gtacggaccg gttcgcaccg cccgagcccg gctccggaaa ccgatcttcc
      961 ctttgcggcc ggagcgcgac gggagtggaa ttgcgtacag gggacaggag aacccgtcac
     1021 tcccggagga ccggaggaaa ggacgcgcca tgagcgacac cgactggggg gacgacgtct
     1081 accagcccca ggaacccgag gcttccgatc ccgacgaaca gctcggcgcc gaggacacgc
     1141 tcatggatcc gtccggcacc ggcgaccccc tggacgaggg gtattcgccg ccggaccgcc
     1201 cgtgggtggt cgacggactc ggcaccacgg ccgacgagcg gaacaccggg gagtccctcg
     1261 aaacgcggct gaccagggaa gtgcccgagc cggacggttc cgccggcgac ggtgtgggcg
     1321 atctggtgga cggcgacgga gagccctggg acgacgaggt gggcgtcgcc cgcgccgggc
     1381 ggctcaccca gcaggccgac gggcgcgtcc cggccaccct caccgccagt gacgtgggca
     1441 tcgacggcgc ggcggcgtcg gccgaggagg ccgcgatgca tgtgatcccc gagccggggg
     1501 acgacgacgc gctcctggag gccggcccgc cgcccgccgc ccccggttcc ggccccgccg
     1561 gcggcggcac ggcagccccg ccgccgtcct gagctgaccg gtcggcgagc gcccgcccct
     1621 gaacgcggcg gtccccgggc ccgtactccc ctgaccgaag cgcacaccgg cccccacgca
     1681 ctcccccgta ccgcacccac cgcacgcacg gacgtactcg caccccagga gcgcaccaat
     1741 gagcggcacc gcccagatcg gcgtcaccgg actcgcggtc atgggccgca acctggcccg
     1801 gaacttcgcc cgcaacggct tcaccgtcgc cgtgcacaac cgcaccacgt cccggacgga
     1861 cgccctggtc gaggagttcg gcgacgaggg cacgttcgtg gccgcgcaca cgcccgagga
     1921 gttcgtcgcc gcgctggagc ggccccggcg tctggtgatc atggtgaagg cgggcgaccc
     1981 caccgacgcg gtgatccagg agttcgcgcc gctgctggag gagggcgacg tcatcatcga
     2041 cggcgggaac gcccacttcg aggacacccg ccgccgtgag aaggagctgc gcgagcgcgg
     2101 gatccacttc gtcggggtgg ggatctccgg tggcgaggag ggcgcgctga acggaccgag
     2161 catcatgccg ggcggctcgg cggagtccta cgcgtcgctg gggccgatgc tggagaagat
     2221 cgccgcgcag gcgaaggacg gcacgccgtg caccacgcac atcggtccgg acggcgcggg
     2281 ccacttcgtg aagatggtgc acaacggcat cgagtacgcg gacatgcagc tgatcgccga
     2341 ggcgtacgac ctgctgcgcg cggtggccgg gtactccccc gcgcagatcg ccgacgtctt
     2401 ccgtacgtgg aacaccggcc ggctggattc gtatctgatc gagatcacgg cggaggtcct
     2461 cgcccatacg gacgcggcga ccgggaagcc gttcgtcgac atcgtgcagg accgggcgga
     2521 gcagaagggc accgggcgct ggaccgtaca gatcgcgctc gacctcgggg tcccggtgtc
     2581 gggcatcgcg gaggcggtct tcgcccgctc gctgtccggc cacgccgatc tgcgggaggc
     2641 ggcccgcggt ctcccggggc ccaccccgaa gccgctggac ggggcggcgg cagcggcgtt
     2701 cgcggaccag gtggaacagg ccctgtacgc ctccaaggtg gtctcgtaca cgcagggttt
     2761 ccaccagatc agggcgggcg gcgagaccta cgactgggac atcgatctcg gctcggtggc
     2821 ggcgatctgg cgggcgggct gcatcatccg ggcggcgttc ctcgaccgca tccgcgccgc
     2881 ctacgactcc cggcccgacc tggtcagcct gctctccgac aaggagttcg ggcaggagat
     2941 cggcgcggcg caggacgact ggcgggcggt ggtggccgag gccgtacgcc agggcgtgcc
     3001 gaccccgggc ttcgccgccg cgctctcgta ctacgacggg ctgcgcgcgg accggctacc
     3061 cgcggcgctc acccagggcc agcgggactt cttcggggcg cacacctacc ggcgcacgga
     3121 ccgggagggc tcgttccaca cgctctgggg cggcgacagg accgaagtgg aaagctgacc
     3181 tgtccgcgcc cgggtccggc ccgcgagctc agcagtagag ccgtcgcaga tagggccacg
     3241 tctgctgacc gaccttgccg tcctcggcca gggtggtgcg gcaggcgtag ttgacgccct
     3301 tctggaactt cttcgccgcg gcctgggagt tgcggccgaa gatgccgtcg atcgtccccg
     3361 ggttgtggcc ctggtactgg aggaggcact gggcctcctt gccggcggcg gtcacctgcg
     3421 aggtggaggg gacgaccgtg gttccgtcgt agtagccggc cgagagccgg ccggcggcgc
     3481 tcatcttcac atcgcacggg taggccgcgg cagcggcggc gcctgtctcg gccgcggcgg
     3541 cgacgccgcc ggtggccttg cctcccagca ggacgaggga ggcgaacacg atcgcggtgc
     3601 gcctgcgggg tgcggtgagt gccatggaag ggctcctgaa agggtgcgtg gtcggcatcc
     3661 ccggccatgg gtgccttgcc cggtcgggca acccgactgt cctgtgaaca cggctgtccc
     3721 gggtacctcg aacagtccag gttgcgcgtc cgccgatcgt cggccggcgg gccgtcgccg
     3781 ctgacccgca cggtccaccc cgggtgcggc cggacccagg gcgtggctta gtggctgcga
     3841 gtcctgttcg tcgttcgtcg aagccccgtt tcaccgtcgt agtggagtgc gcgcatgccc
     3901 gacgccgtgc ctgaccccgt ctccgtccag cccgtcgtgg cgccgttgac cagcgcggcg
     3961 ctgttcctgg tcggcacgat cgagccgggc ggcgaaggcg cggtgcggga cgtgctgccg
     4021 gatctcgcgg ccgtcgcccg gtcgctcggg ttccggtacc cggactccgg cctcgcctgt
     4081 gtggcgggct tcggctccag cgcctgggac cggttgttcg cggggccccg cccctcgcac
     4141 ctccacccgt tcccggagct gcgagggcct ctgcaccacg ccccggcgac cccgggtgac
     4201 gtgctgctgc acatccgggc cgagcggatg gacgtctgct acgcgtgggc ggcccagttg
     4261 ctcgacaagc tcggcggggc cctgcggatc gtggacgaga cgcacggctt ccgctacctc
     4321 gaccaccggg atctgctcgg cttcgtggac ggtacggaga acccggtcgg cgacgacgcc
     4381 cgttcggcgg cgctggtcgg cgcggacgac gacccggact tcgagggcgg cagctacgtc
     4441 gtcgtgcaga agtatctgca cgacctgacg tcctggaacg cgctcaccgt cgaggagcag
     4501 gaacgcgtca tcggccgcac caagctcgac gacgtggagt tccccgacga ggacaagccc
     4561 gccgactccc acctcgcgct caacaccatc accgacccgg acggcaccga acgcgacatc
     4621 ctgcgggcga acatgccgtt cgggagcttc gggaagggcg agttcggtac gtacttcatc
     4681 gggtacgccg ccgatcccga cgtgaccgag cggatgcttc gcaacatgtt cctcggcgat
     4741 ccgcccggga cgcacgaccg catcctcgac ttctccaccg cggtcacggg ctcgctcttc
     4801 ttcgcccctc gcgccgactt cctcgacgcc ccgccgcccc tgcccgcccc ggcccgctcc
     4861 ggggaccggc cggaaccggt gcccgtgccg gtcctgcagg agccgggacc cgcccctgcc
     4921 tccgcccggc aggagccggt cgcggccggc cccgaccacg gttcgctgcg catcggcagc
     4981 ctgcaagaaa gtgcccagtg atgaacaatc tgcaccgcga actcgcaccg gtcaccccgt
     5041 ccgcctggga cgagatcgag gaggaggccc gccgcacctt ccgccgccat gtcgccggcc
     5101 gccgggtcgt ggacgtgtcc gatccggacg gccccacgct gtccgccgtg ggcgacggcc
     5161 atctgcgcga catcgatccg cccaccccgg acgtggtggc gcgggcccgc acctcgatgc
     5221 cggtcatcga gtggcgggtt ccgttcacgg tgacccgtca ggccgtggac gacgtggagc
     5281 gcggctccgc cgacagcgac tggcagcccg tcaaggacgc ggcgcgcacc tgtgcgttcg
     5341 ccgaggacat ggcgatcatc gacggatacg gggcggccgg gatcaccggc ctgcgggacg
     5401 gttcctcgca cgagccactg ccgctgcccg ccgacgcgcg ggactatccg gtggcggtca
     5461 gccaggccgt gacgcggctg cggctggccg gtgtcgacgg accgtaccgg ctgctgctcg
     5521 gcgcggacgc gttcaccgag gcggccgaga cctcggacca cgggtatccg gtcaagacgc
     5581 atctgagccg cctggtggac gacgagatcc tgtgggcgcc cgcggtcaag ggcggggtac
     5641 tgctctccac ccggggcgga gacttcgaac tctgcctggg ccaggacctg tcgatcggct
     5701 acgcggacca cgacgccacc agcatccacc tctacttcca gcaggccttc accttccgga
     5761 tgctgacgcc ggaggccgtg gtcggtctga tcgcctgacc ccgggggcag ccatcaggga
     5821 cgctgaccgt atgacgcaga cgacttccgc accccaccac gaggacggca cggcaccacc
     5881 cgccgcccgc ttcgacgccg tcgtcgaagc ggccgtccag caaggactgc tgggcgaggg
     5941 ctgcccggtc gccggattca tcgacacgga gggggtgcgt gcctccgtcg gaaccctgca
     6001 cgacgccttc gccgccacac ccggggtact gcacaccttc gcggccaagg cctgttccct
     6061 gatcccggtg ctgcggctgc tcgccgactg cggcatgggg tgcgaggtgg ccagccccgg
     6121 tgaactgcgc ctcgccctgg aggccgggtt ccccgcgtcc cgcatcgtcc tggactcccc
     6181 cgccaagacc cgcgaggaga tccggctggc gctcgccctc ggcgtggccg tcaacgccga
     6241 cagcctcgac gaactgcgcc gcgtcgacgc cctccggaac ccgggcaccg cctcggtgct
     6301 cgggctgcgg gtcaaccctc aggtcggcgg tggcgcgatc ggggcgatga gcacggcgac
     6361 ggacacctcg aagttcgggg tggcgctgcg cgatgcgggg gcccgcgagg cgatcgtgac
     6421 ggccttccgg gaacggccgt ggctgacccg gctgcatgcc catgtgggct cccagggctg
     6481 cccgttggag ctgatcgcgg ccggggtggc acagacgtac gaactggccg aggagatcaa
     6541 cgcgacgctg ggcgtccggc aggtcaccag tctcgacatc ggcggcggac tcccggtcaa
     6601 cttcgccgac gagaccgtgc accccaccta cgcggaccat gtggcggccc tgacggcggc
     6661 cgcccccggc ctcttctccg ggcactatgg gctggtcacg gagttcggcc ggtcgctgct
     6721 ggcgaagaac gggttcatcg gggcccgggt ggagtacacg aaggacgccg ggggccgccg
     6781 tatcgcgctc acccacgcgg gagcgcagac cgcgacccgt accgtcctga tgcccgacgc
     6841 ctggccgttg cggatcgggg cgttcggccc ggacggcacg ccgaagggcg gcccggtgct
     6901 ggcgcaggac atcgcgggac cgtgctgctt cgcgggcgac gtggtggcgt acggcagaga
     6961 gctgccggaa ctggcccagg acgatgtggt ggtgctgtac gacaccgggg cgtactactt
     7021 ctccaccccg tgggcgtaca acagcctccc gcggccggcg gtgtacgggt tccgggtggc
     7081 ggcgggggtg gggcgcgaca gggctgcggt gacgttcgcg acggtgcggg acgcgcagtc
     7141 gatggacgcc atcgcggcgg agagcggtct cgcccacgcg gacgcgctgg tcgggcggag
     7201 cgactgaccg gggcgcctga ccaggacgga tgcctgggaa ggatgcctgg ggcggacttc
     7261 tgggaaacgt acgccgcccg gcaccccggc acccctcggc ttcaccgggg cgggccgggg
     7321 agggccggga agcgccgggc gacggtcggt catcgccacc ggggccggca ccgtctccgg
     7381 taccggcccc cgctcacgtc ccggcgggct ggggctcggg ttccggatcc ggtacggggt
     7441 cggggcccgg cggctgcggg atcgggtcgg gtgcggggcc gggcggcacg ggcccgggac
     7501 ccggcggcac gggcggcgga ccgggcaccg gagtgggcgg gcccggtacg ggcggaggcg
     7561 tcggcgaggg cggtacgggg tcggggtacg ggtgggtcat cgcgatcctc cacaacggca
     7621 cgaaaaggac agatggagct ttcctccacc gtctcccggt gcggctgccc gcgcctgccg
     7681 agtccacaca tgcgcccggc gacggcggtg cgtgcggccg tgtgccgacc cggccggacc
     7741 gggcagaact ccggcagggc tcccggcccc gtccgggatc cacgaccggc accgtcgaca
     7801 cgaaaggcgt ccccatgcct ctgccgagca tcctcgccgt ccacgcccat ccggacgacg
     7861 agtccttgtt ctgcggaggc gtactggccc ggcacgcggc ggccggcgcc cgcacggcgg
     7921 tggtcaccgc gacctgggct ccgggcacgc accgggccgc cgagctggcc cgggccctgg
     7981 acgccctggg cgcgggcgcc ccgcgcctgc tcggctacgc ggacgcccgg gtgccggagt
     8041 cggccccggg caggccccgg ttcctcgacg ctccgctgga cgaaaccgtg gaggcggtgg
     8101 tcggccacat ccgggacgtc cggccggagg tcgtgatcac gggggacgcc tacggaggca
     8161 tgaccggcca cccggaccac gtacacgccc accggatcac cgcgctggcg gtccgggccg
     8221 ccgggctccc ccagtacggt ccgcatgccg gtgatccctg gcagccgagc gccctctacc
     8281 tggccacgca cccccgctcg gcggccgttc cggtgggtgg acggctggcc cgggcgggca
     8341 ccccggccgg cgcgctgtac tgctcggagg acgcgtggat caccacgacg gtcgacgtcg
     8401 gcccctggct cccgcagaag ctcgccgcga tcctcgccca ccgcagcgag gtggaacggg
     8461 gggcggcacc gggccgaatc gccgctctgc ctccggcggt tcagcgggag gtgctgggca
     8521 ccgagtggta catccgccag gacctcacgc cccggagcgg gtccgccacg gagctgagcg
     8581 cctgacgggc gcgtgcgatg cggacggagg gctcagaaca gctcaggatg gtgcctgagc
     8641 tgccgctccg cctcccgcac cagcccgcct ggcgccgcgg gcgcctcgtc ggggtggcgc
     8701 tgcgcccact tcgccgcgta cgggcacagc ggcacgacgg gcacgccctc acgggcggcc
     8761 atggcgtaga actccctcac gagcgcgccc gcgatgccct tgccctcgtg ttcgggccgg
     8821 accacggtgt gcacggccac gagcgcgccg ggagccgggt ccatgacgaa gtacgcgatg
     8881 gtcccggccg acgtgccgtc ctcgtacgcc acgaggttgc cgtgctcccg gtcgtccctg
     8941 atctccactg ccgcgttctc cgccatggtt cctgccctct cgttcgtgga ccgcgcacca
     9001 ccgtgctcgt cacggaagcg cggacgacgg cctcagccgc ccgggacgac ggcctgcggg
     9061 ctccgctccg tacggctccc gggaaccggc gccgaggcgt cgtcaccgag tgccacgatc
     9121 cggttgtccg cgtccacgtg cacgacccgc ggcacgagtg cacgggcctc ggcgtcgtcg
     9181 acctgcgcgt agctgatcag gatgaccagg tcaccggggt gcacgaggtg tgcggccgcg
     9241 ccgttgatcc cgatgacacc ggagccgcgc tcgccctcga tgacgtacgt ctccaggcgg
     9301 gcgccgttgt cgatgtcaac gatatggacc aactcgccgg gcagcaggtc ggcggcctcc
     9361 atcagggcgg cgtcgatcgt cacggaaccg acatagtgca ggtcggcctg ggtgacggtg
     9421 gcccggtgga tcttggactt gaacagagtg cgcagcactg tggactcctc tgagacggct
     9481 ccctgcctgc tttctgcagg tcaagggcgt ccccgacttt acaccgacac ccccgtccga
     9541 tgtttgtgag gaacatcgct ccggccgggc gatacgggtt ccccacctgg gctttccctt
     9601 ggcaccggcg tctgttggga cgatcgtccc ggaaggctca gggactcgtg ctgaccgaat
     9661 gctgacttac ctggcgatgc gtcaggtcct cacaagacca ccccatcgcc cgggacgact
     9721 acatgattcc ggcctggact ctcgaccctg cgggttggcg tggcgatcct gtccctgttc
     9781 ctggtgtgac ggtgcgagag gagccagtcg gtggcgtcct cctgttcccg tcggtggacg
     9841 ggaaccaggc accccccgcc cagccgcaac gaagcggtaa ccgacgtcgg ttcctcatgt
     9901 acgcgaagca ggcaagggga tccgccgtga gcccccggtt cgaggagagc gttggtcaag
     9961 cctccagagt ggcacggatg acgacgagtg agccttcgca ggtcgggcgg gtacgcaggt
    10021 ggccgaagcg ttcgtcccag actcccgctt cgaggtcgcg gcggagggtg cggttgaagc
    10081 attcgcgggc gtcgtcgtcc acgaagctcc aggcggagca cgctgccggg cggcggggtc
    10141 gaggagcatt tcggggcggc cgtagtccgg ctgcctcatc atgggcaacc gttgcgcggt
    10201 gctgaagtcg accgatgacg ccgacacgag caccgatcac agccgtctgg tgatcaacga
    10261 taccgtctac gcgccgaccg ctgccgtcga cctgccgatg agccgggtca gctcgcaggt
    10321 cgtcgcccgg accatcgatc tcggcatcag cccggccacc gggtacctcc gcccggtgat
    10381 cgagatccca ccggagccga tgctggtcac gacctatccg gccgtaatcg cgaaaccggc
    10441 gtcggtcacc gcggtcaccg ggttcacggc cccggctccc ggcgcgcccg tcgacgtcac
    10501 gaacgccacc atccccggcg atgatcaggc atccctcacg ctcggcagct acacccggcc
    10561 cgcaccggcg acgaccggac cgctcgacca cgtcgtcctg caggtggcgc accacgagga
    10621 cggcgatgtg gggtcggtaa ggctgttggt ggacttcccc agcagcacct gccccagcgt
    10681 cgactcgctg accttgccgg tccgtggccg tcccgaagga cgaccgccac cacgcggggt
    10741 gtggtggccc tgtatgacgc cctcggcatt gcgaccctga gtagccgcgg gggacgac
//
