LOCUS       NZ_KL573564            17991 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces baarnensis strain NRRL B-2842 P144_Doro1_scaffold26,
            whole genome shotgun sequence.
ACCESSION   NZ_KL573564
VERSION     NZ_KL573564.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces baarnensis
  ORGANISM  Streptomyces baarnensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 103308)
  AUTHORS   Doroghazi,J.R., Albright,J.C., Goering,A.W., Ju,K.-S., Haines,R.R.,
            Tchalukov,K.A., Labeda,D.P., Kelleher,N.L. and Metcalf,W.W.
  TITLE     A Roadmap for Natural Product Discovery Based on Large-Scale
            Genomics and Metabolomics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 103308)
  AUTHORS   Doroghazi,J.R., Ju,K.-S. and Metcalf,W.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2014) Institute for Genomic Biology, University of
            Illinois at Urbana-Champaign, 3109 IGB, MC-195, Urbana, IL 61801,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: IDBA UD v. 1.0.9
            Genome Coverage                   :: 74x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 02:19:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,395
            CDSs (total)                      :: 7,345
            Genes (coding)                    :: 7,173
            CDSs (with protein)               :: 7,173
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 55 of 173
            Pseudo Genes (incomplete)         :: 138 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 27 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 85317
            Orig. end                         :: 103308
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KL573564.1.
            Bacteria available from NRRL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..17991
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..17991
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..17991
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:12797](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12797
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    complement(87..101)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.9"
                     /tool="antismash"
     misc_feature    200..218
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.25"
                     /tool="antismash"
     gene            218..1069
                     /gene="otsB"
                     /locus_tag="BG84_RS0130645"
     CDS             218..1069
                     /EC_number="3.1.3.12"
                     /GO_function="GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="otsB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958814.1"
                     /locus_tag="BG84_RS0130645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="trehalose-phosphatase"
                     /protein_id="WP_030087110.1"
                     /transl_table=11
                     /translation="MGSHPESLPNPTTPAGREGLDAVLARPDRAVVALDFDGTLADIVP
                     DPEQARAHPGTVAALAALAPKVAAVAVITGRPAGVAVRYGGFAGVPGLEHLVVLGHYGA
                     ERWDAVTGTVHAPAPHPGVTAVRAELPGVLHEFGAWNGTWIEEKGQAVAVHTRRAEDPQ
                     AAFESLRGPLGELAARHGLIVEPGRLVLELRPPGMDKGVALAGFVAELDAESVIYAGDD
                     LGDLAAFAAVEKLRSEGPDGIPGLLVCSGSTEVPELAERADLSVPGPAAVVAFLAALAE
                     RI"
     gene            complement(1089..2480)
                     /locus_tag="BG84_RS0130650"
     CDS             complement(1089..2480)
                     /EC_number="2.4.1.15"
                     /GO_function="GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012379742.1"
                     /locus_tag="BG84_RS0130650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="WP_030087112.1"
                     /transl_table=11
                     /translation="MVSEHTPSSNAQVLVASNRGPVTYTLGEDGTLDSKRGGGGLVSGL
                     SAVDDKLWVCAALGDGDREAVRRGVGEPGVRMLDIDAEVHADAYNGIANSVLWFVHHLL
                     YQTPVEPVFDTEFRRQWAAYETYNRAFAEALAQEAGPGAAVLVQDYHLALVPGMLRELR
                     PDLRIGHFSHTPWAPVDYFRLLPDDIAEQLLRGILGADRAAFLTRRWADAFIGCCTEIL
                     GGTGRTRIGVHGLGADADFLRRRSHEADVDERMAALREQVGEGRKTIVRVDRTELSKNI
                     VRGLHAYRTLLDTRPEWRERVVHIAFAYPSRQDLAVYRDYTAAVQTLATEINAAYGTES
                     WTPVVLHVKDDFARSLAAYRLADVALVNPIRDGMNLVAKEVPVVSDHGCALVLSREAGA
                     YEELGEDAIVVNPYDVTGTAEALHEALTMSGDERSGRTKRLAEAATALPPQQWFLDQLE
                     ALREG"
     gene            complement(2545..3495)
                     /locus_tag="BG84_RS0130655"
     CDS             complement(2545..3495)
                     /EC_number="2.4.1.266"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511020.1"
                     /locus_tag="BG84_RS0130655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="WP_030087114.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 3.4e-11,
                     bitscore: 35.9, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLEEVERWLTRRSWSSGDRPLNRLTDARDADPRRTSVSVVLPALN
                     EEATVGAIVATIRRELMEKVRLVDELVVIDSGSTDATAAVARAAGARVVHRDAILPRIP
                     ALPGKGEVLWRSLLVTSGEIVCFVDADLKDFSADFVSGTIGPLLTDPTVQFVKAMYDRP
                     LGDRAGQGGRVTELVARPLLNLHWPQLAGFVQPLGGEYAVRRSLLERLPFPVGYGVELG
                     LLVDALHTVGLDALGQVDVGVRIHRHQDGQALGRMAAAIYRTAQLRLSRGHLVRPALTQ
                     FERGEDGFVPRTHAVDTEERPPMREIEEYAERHAA"
     regulatory      3649..3776
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /regulatory_class="riboswitch"
     gene            3795..5090
                     /gene="thrC"
                     /locus_tag="BG84_RS0130660"
     CDS             3795..5090
                     /EC_number="4.2.3.1"
                     /GO_function="GO:0004795 - threonine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thrC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010056010.1"
                     /locus_tag="BG84_RS0130660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="threonine synthase"
                     /protein_id="WP_030087116.1"
                     /sec_met_domain="PALP (E-value: 5.6e-63, bitscore: 205.6,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVQTVAGNATTAPVDLGPAAALSCRECGERFELGPLFACASCFG
                     PLEVAYDLPTGSPEELRKRIEAGPDNIWRYAPLLPVPSGVAEKPNINPGFTKLVKADNL
                     ARELGVTGGLYVKDDSGNPTHSFKDRVVAIAVEAARAFGFTTLSCSSTGNLAGAVGAAA
                     ARAGLRSCVFIPHDLEQGKVVMAAVYGGELVGIEGNYDDVNRFCSELIGDPLGEGWGFV
                     NVNLRPYYGEGSKTLAYEICEQLGWRLPDQIVIPIASGSQLTKIDKGLQELIKLGLVED
                     KPYKIFGAQAEGCSPVSAAFKAGHDVVRPQKPNTIAKSLAIGNPADGPYVLDIARRTGG
                     AVEDVTDEQVVDAIKLLARTEGIFGETAGGVTLGVTKKLIEAGVIDPTLTTVVVNTGDG
                     LKTLDAVSATSQATATIKPSLDAFRAAGLGTA"
     gene            5126..5404
                     /locus_tag="BG84_RS0130665"
     CDS             5126..5404
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007265553.1"
                     /locus_tag="BG84_RS0130665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MoaD/ThiS family protein"
                     /protein_id="WP_003967347.1"
                     /transl_table=11
                     /translation="MSVKVRIPTILRTYTGGQAEVPAEGAKLSEVIESLEKDHPGIAAR
                     VLDDQGKLRRFVNVYVNDDDVRFEGGLDAATPDGAGVSIIPAVAGGC"
     gene            5850..6056
                     /locus_tag="BG84_RS0130670"
     CDS             5850..6056
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1255: cold-shock
                     DNA-binding domain protein"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967346.1"
                     /locus_tag="BG84_RS0130670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cold-shock protein"
                     /protein_id="WP_003967346.1"
                     /transl_table=11
                     /translation="MAQGTVKWFNAEKGYGFIAVDGGADVFVHYSAIQMDGYRTLEEGQ
                     RVEFEISQGQKGPQADMVKLAVG"
     misc_feature    complement(5898..5911)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     misc_feature    6284..6299
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.7"
                     /tool="antismash"
     misc_feature    complement(6287..6302)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.11"
                     /tool="antismash"
     gene            6454..>7204
                     /gene="groEL"
                     /locus_tag="BG84_RS0130675"
     CDS             6454..>7204
                     /codon_start=1
                     /gene="groEL"
                     /gene_functions="other (smcogs) SMCOG1299: chaperonin
                     GroEL"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /locus_tag="BG84_RS0130675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaperonin GroEL"
                     /protein_id="WP_030087120.1"
                     /transl_table=11
                     /translation="MAKIIAFDEEARRGLERGMNQLADAVKVTLGPKGRNVVLEKKWGA
                     PTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREGLR
                     NVAAGANPMALKRGIEKAVEAVSAALLEQAKDVETKEQIASTASISAADTEIGAKIAEA
                     MDKVGKEGVITVEESQTFGLELELTEGMRFDKGYISAYFATDMERMEASLDDPYILIVN
                     SKIGSVKDLIPLLEKVMQSGKPLLIIAE"
     assembly_gap    7205..7464
                     /estimated_length=260
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7465..>7873
                     /gene="groEL"
                     /locus_tag="BG84_RS0130680"
                     /pseudo=""
     CDS             7467..>7873
                     /codon_start=2
                     /gene="groEL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713461.1"
                     /locus_tag="BG84_RS0130680"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES; misfolded
                     proteins enter the barrel where they are refolded when
                     GroES binds; many bacteria have multiple copies of the
                     groEL gene which are active under different environmental
                     conditions; the B.japonicum protein in this cluster is
                     expressed constitutively; in Rhodobacter, Corynebacterium
                     and Rhizobium this protein is essential for growth;
                     incomplete; too short partial abutting assembly gap;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="chaperonin GroEL"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEALSTLVVNKIKGTFKSVAVKAPGFGDRRKAMLADIAILTGGTV
                     ISEEVGLKLENAGLDLLGRARKVVITKDETTIVDGAGDSDQVQGRVNQIRAEIENSDSD
                     YDREKLQERLAKLAGGVAVIKAGAATEVELKE"
     assembly_gap    7874..8103
                     /estimated_length=230
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8104..8593
                     /locus_tag="BG84_RS0130685"
     CDS             <8106..8593
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713461.1"
                     /locus_tag="BG84_RS0130685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="WP_324605518.1"
                     /transl_table=11
                     /translation="AGAATEVELKERKHRIEDAVRNAKAAVEEGIVAGGGVALLQASAV
                     FEKLDLTGDEATGANAVKLALEAPLKQIAVNGGLEGGVVVEKVRNLPIGHGLNAATGEY
                     VDMIAEGILDPAKVTRSALQNAASIAALFLTTEAVIADKPEKAGAAAPGGMPGGDMDF"
     gene            8802..9281
                     /locus_tag="BG84_RS0130690"
     CDS             8802..9281
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010056007.1"
                     /locus_tag="BG84_RS0130690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_032787684.1"
                     /transl_table=11
                     /translation="MTKDGKGASFVLGGAVLFVLGLFVVVTGTGLSGALGMRVETFGRI
                     DCDDTRTKKGQTVWHCFGESPVQQRANEAERERVAREALRAHVDGVPRPAETGRTRILF
                     ADHDGRTDPETITATQVFDGGRWYAHSSMVVGYGMIPLLLGAGAAAWGGYRLRRR"
     misc_feature    9537..9552
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 23.0"
                     /tool="antismash"
     gene            9828..9950
                     /locus_tag="BG84_RS1000000139430"
     CDS             9828..9950
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489031.1"
                     /locus_tag="BG84_RS1000000139430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="keywimysin-related RiPP"
                     /protein_id="WP_018511015.1"
                     /transl_table=11
                     /translation="MKQQKKAYVKPSLFKQGDFSKKTAGYFYGSYKEYWVRRII"
     CDS_motif       9828..9899
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MKQQKKAYVKPSLFKQGDFSKKTA"
                     /locus_tag="BG84_RS1000000139430_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       9900..9950
                     /prepeptide="core"
                     /locus_tag="BG84_RS1000000139430_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="0.20"
                     /molecular_weight="2082.4"
                     /monoisotopic_mass="2081.0"
                     /core_sequence="GYFYGSYKEYWVRRII"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MKQQKKAYVKPSLFKQGDFSKKTA"
                     /leader_location="[9827:9899](+)"
     gene            10001..12043
                     /locus_tag="BG84_RS0130700"
     CDS             10001..12043
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610346.1"
                     /locus_tag="BG84_RS0130700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_032790983.1"
                     /sec_met_domain="Asn_synthase (E-value: 6.3e-25, bitscore:
                     81.1, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGQLRDYFVVLPDSVAGAAVAGRLPPGAGGGTVVAHASGRPWVL
                     ARPLVRRVSHLRRGDDALVLIGPDRVSDAVLSGLLEGVRDRAGLERRLARLPGLHHVIA
                     RLSGQTWIRGTASGLRRIYHARHPEAGTIASDRPAVLARLIGAPLDDGALALRMLDFLP
                     HPLSRRVVWRGVHETVAGYGLALPAAAPGTAAVPGPTEHRWWEPPPAELPLAEGAQALG
                     DAIAASVRAHVDGLDRISCELSGGMDSTALTFAARATGPAELALLTVAARDRYSEDETW
                     ARRAVEAARGGGGGARGGEATRSGGAARGSGGKPDSNAARTRDTGRKGGDVTGLDHHII
                     PADDAPYFYADLASTAAELNDEPLPVAPGRARAHLLLSRAHATGSRYHLTGYGGDELFL
                     GLPHAYQDLFHGNPLTAWNHLSGLRHQLGWPFLPTLKALLNRSTFPRWLAGAITAEPQP
                     VTRTPLLSWGVRQSLRPWFTEHANALIAEEFRAAAEHAEPIDPWRGRHVDIDAVRMGAR
                     HFQAMEDIGMTIGLPVAAPFYDDRVLEATLAVRLAERISPWRYKPLLVEAMRGVVPDAL
                     LARTTKDHMSSDEHQGLRQHASDLADLWTGSRLAEHGLVDSRHLLRLAAEPFSPVLVEH
                     SVSSTVAGETWLRTAENAWPTPTPTPSQSPSPPPHRTSTTSEATL"
     aSDomain        12040..12285
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_BG84_RS0130705_Stand_Alone_Lasso_RRE.
                     1"
                     /evalue="1.30E-26"
                     /identifier="RREFam002.1"
                     /label="BG84_RS0130705"
                     /locus_tag="BG84_RS0130705"
                     /protein_end="82"
                     /protein_start="0"
                     /score="83.4"
                     /tool="antismash"
                     /translation="MKLRKGIAVTTTEYGGVLLDEKDGSYWQLNDTSIIVVDTLAAGHT
                     PDIAVERILAAFDVERAEAESDVAELTRQLVDAKILR"
     gene            12040..12291
                     /locus_tag="BG84_RS0130705"
     CDS             12040..12291
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006126488.1"
                     /locus_tag="BG84_RS0130705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_030087127.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 4.2e-26,
                     bitscore: 83.4, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.2e-13, bitscore: 43.7,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLRKGIAVTTTEYGGVLLDEKDGSYWQLNDTSIIVVDTLAAGHT
                     PDIAVERILAAFDVERAEAESDVAELTRQLVDAKILRP"
     gene            12288..12797
                     /locus_tag="BG84_RS0130710"
     CDS             12288..12797
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010056001.1"
                     /locus_tag="BG84_RS0130710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_030087128.1"
                     /sec_met_domain="PF13471 (E-value: 1.9e-25, bitscore: 81.7,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEMTMPRRGAGAGGVRLRTAIVAAFVLARLKPGRLRRVLARCA
                     RGARPASYAETMEVLEAVVATSRRCASRYGCLPRSVAVALACRMSGVWPDWCAGVRSAP
                     PFAPHAWLQAEGRTVGEQAEPADLRPLMVVAAAAVTAGGGVRDERGVGDDGGGSESRGG
                     GGRQRA"
     gene            12727..14694
                     /locus_tag="BG84_RS0130715"
     CDS             12727..14694
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958803.1"
                     /locus_tag="BG84_RS0130715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_030087129.1"
                     /transl_table=11
                     /translation="MSAVSGTTGAEARAGAEAAGSGPEDAGIPAAGPAGEPVGPGQTAG
                     EAAGPERPTGEAPAPETPAGGSPADTTGNPAQGKSGPSPLRLLLARVRPHRGVLIRAGL
                     LSLTGSAAGLAMPLMAKYAVDAFAADRSPVAPLVALTALVLAGACISAYGRYLMARTGE
                     GVVLRARDQLVARIMRLKVPAVDRLTPGDLQSRVTSDTTLLRTVLSSGLVESFNSVLML
                     LGTVAFMAYMDVTLLGVTLVVIVGIGAVTALLMPRIQRAQLRAQESVGAMGAALDRVLQ
                     AFRTVKASGAEERETAAVAEAARHAHDRGVSVAKWSSVSDVTMMMSIQLAFLAVLGVGG
                     ARVASGSLEISSLIAFLLYLFYLMGPIGGLVEGWTGLQSGLAAVRRIDEVESLPGETST
                     GSGAVEPEGGPLGVAFQDVTFGYGDERAPAHKGVTFDVPAGGLVALVGPSGAGKSTVFS
                     LLERFYDHDGGTITVGGRDIRDWPLAELRASLAYVEQDAPVLAGTLRENLLFAAPEAEE
                     RELTAAVARTRLDALVDRLPDGLDTAVGHRGVTLSGGERQRIAIARALLRRPRLLLLDE
                     VTSQLDAVNEQALRDVILEMAEQATVLVIAHRLSTVRHADRIVVLEEGRVRTAGTHEEL
                     IAGDDLYRELATTQLAAEVA"
     gene            14694..15065
                     /locus_tag="BG84_RS1000000139435"
     CDS             14694..15065
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BG84_RS1000000139435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158688328.1"
                     /transl_table=11
                     /translation="MAGRRRRDDGSAANAMMGDKGTGTGTIESPETPATPATPATPATP
                     ATPATLYGPVSLGFGVIALIAAFFLGFFALLAGSLAITFGVLGLISAYNRPQCLAGLVL
                     GAVGVLYPLSFLFAFTGGF"
     gene            complement(15091..15678)
                     /locus_tag="BG84_RS0130725"
     CDS             complement(15091..15678)
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762767.1"
                     /locus_tag="BG84_RS0130725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Uma2 family endonuclease"
                     /protein_id="WP_030087132.1"
                     /transl_table=11
                     /translation="MTPSSADHAPHAQMSVEEFEQLARTAPETVTLELINGKLEVKPVP
                     DGDHDEIIMWVAMQCMQQQPELRLYRERGLRIGGYRNGRARPDGTLARAGRFAGDGEWS
                     SPDRVLMTVEVTSHDHDTDHRDRTEKRDGYAAAGIPVYLLIDRDHDALVVYSEPENGRY
                     RQRTTYEYGKAAALPHPVDITLDTEELKDYAR"
     gene            complement(15735..16553)
                     /locus_tag="BG84_RS0130730"
     CDS             complement(15735..16553)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958801.1"
                     /locus_tag="BG84_RS0130730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_030087134.1"
                     /transl_table=11
                     /translation="MANPGYGKRSAPGERPRTDRDFAHLLPRDASVAAYIDRLPDGSDI
                     SVKTLAKHLPYGQCALRTTLNRLQDAGHLRRGSECVITDEVMLWVTRTFFSRTAREDAW
                     WNAYTRGDVPPDEPQRPTRSRAFILLAALGRTAPALTLSAADCVRLEPQVGEWIARGAT
                     EAELLHALTSGLPSPVHCAARLVERRLRDKLPPEPVLPGPRTVLRTLECGECGVPGPPE
                     ALLGGLCRPCRGPSPEPPVGPPGTVQPPGVRDRMNAVRAGLVPRSERTYR"
     misc_feature    complement(16332..16334)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(16914..16929)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
     gene            17011..17511
                     /locus_tag="BG84_RS0130735"
     CDS             17011..17511
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489040.1"
                     /locus_tag="BG84_RS0130735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="WP_030087136.1"
                     /transl_table=11
                     /translation="MTVEEFEEFYARAVARLTGQLYVMTGDLQEAQDVVQEAFVKAWVR
                     RGRLERDGQPEAWIRTVAWRLAVSRWRFRRRSADAWGRESGAPEHVEPPDPGHVVLVDA
                     LRELPAQQRRTLTLHYLCDLTVEQIAGETGLSTSTIKTHLVRGRAALAHRLHDPRIEEA
                     PDV"
     gene            17504..17950
                     /locus_tag="BG84_RS0130740"
     CDS             17504..17950
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958798.1"
                     /locus_tag="BG84_RS0130740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_030087138.1"
                     /transl_table=11
                     /translation="MSEPRDPLRSLFREAGDFGQVRSVADPASSITERGMRAQRRRMAV
                     VAVGVCLVVGGGGAVAVGLLPVRPEPVAPATSPSPSYPSPVPSEGLSTEQSSPPPSPST
                     TLPGGLPTGTGTGAPTGTSPTATKPPPTSESTTSTATDGATSTP"
ORIGIN
        1 gctcgtgtgt gccacgcgcc tcacctcggt tccgtgaatc cgtgatcgtg attccatgaa
       61 tcaattaatg gaatcatgga gtcgtgaggg aatgactgtc aagtgaggca ggccggggag
      121 ggagggggcg ggaagagggg aggtgggttt gccggggtgg cgccccggtc gcggccgaac
      181 ccggcggcag tggccccgta cccgttaggc tcgccccatg ggcagccacc cggaaagtct
      241 tccgaacccg accaccccgg ccggccgcga ggggctcgac gccgtgctcg cccggcccga
      301 ccgcgcggtc gtcgccctcg acttcgacgg cacgctcgcc gacatcgtcc cggaccccga
      361 gcaggcccgc gcccaccccg gcaccgtggc cgcgctcgcc gcgctcgcgc cgaaggtcgc
      421 cgccgtcgcc gtgatcaccg gccgcccggc gggcgtcgcc gtgcgctacg gaggcttcgc
      481 gggcgtgccc ggcctggaac acctcgtggt gctcgggcat tacggggccg agcgctggga
      541 cgccgtcacc ggcaccgtcc acgcgcccgc cccgcatccg ggcgtcaccg ccgtacgcgc
      601 cgaactcccc ggcgtactgc acgagttcgg tgcgtggaat ggcacctgga tcgaggagaa
      661 gggccaggcc gtcgccgtgc acacccggcg cgccgaggac ccgcaggcgg ccttcgagtc
      721 gctgcgcgga cccctcggcg agctggccgc gcggcacggg ctgatcgtcg aaccgggccg
      781 cctggtcctg gagttgcgtc cgcccggcat ggacaagggc gtcgcgctcg ccgggttcgt
      841 cgccgagctg gacgcggagt ccgtgatcta cgcgggcgac gacctcgggg acctcgccgc
      901 gttcgccgcg gtggagaagc tgcgcagcga gggcccggac ggcatccccg gtctcctggt
      961 gtgcagcggc agcaccgagg tgcccgaact ggccgagcgc gccgacctgt cggtgccggg
     1021 accggccgcc gtcgtcgcgt tcctggcggc cctggccgag cggatctgag ccgggccagg
     1081 accccggctc acccctcccg cagcgcctcc agctggtcca ggaaccactg ctgcggcggg
     1141 agcgcggtcg ccgcctccgc gagccgcttc gtgcggcccg accgctcgtc cccgctcatc
     1201 gtcagcgcct cgtgcagggc ctccgccgtg cccgtcacgt cgtacgggtt caccacgatc
     1261 gcgtcctcgc ccagctcctc gtacgccccc gcctcccgcg acagcaccag cgcgcagccg
     1321 tggtcggaga cgacggggac ctccttggcg accaggttca tgccgtcgcg gatcgggttg
     1381 accagcgcca cgtccgccag gcggtacgcg gccagcgagc gggcgaagtc gtccttgacg
     1441 tggaggacca ccggggtcca gctctccgtg ccgtacgccg cgttgatctc cgtggccagg
     1501 gtctgcaccg ccgccgtgta gtcccggtag acggcgaggt cctggcggga ggggtaggcg
     1561 aaggcgatgt ggacgacgcg ctcgcgccac tcggggcggg tgtccagcag tgtgcggtac
     1621 gcgtgcaggc cgcgcacgat gttcttggac agctcggtgc ggtccacccg gacgatcgtc
     1681 ttcctgccct cgcccacctg ctcgcgcagg gccgccatcc gctcgtccac gtccgcctcg
     1741 tgcgagcgcc ggcgcaggaa gtccgcgtcg gccccgaggc cgtgcacccc gatcctggtc
     1801 cggcccgtgc cgccgaggat ctccgtacag cagccgatga acgcgtccgc ccagcgccgg
     1861 gtcaggaacg cggcccggtc cgcgccgagg atgccgcgca gcagctgctc ggcgatgtcg
     1921 tcgggcagca gccggaagta gtccaccggg gcccacgggg tgtgcgagaa gtggccgatc
     1981 cgcaggtccg ggcgcagttc gcggagcatg ccggggacca gggccaggtg gtagtcctgg
     2041 accaggaccg ccgccccggg ccccgcctcc tgtgccagcg cctccgcgaa ggcccggttg
     2101 tacgtctcgt acgcggccca ctgccgccgg aactccgtgt cgaagaccgg ctcgacgggg
     2161 gtctggtaca gcaggtggtg gacgaaccac agcaccgagt tcgcgatgcc gttgtacgcg
     2221 tcggcgtgca cctcggcgtc gatgtccagc atccggacgc ccggctcgcc gactccgcgc
     2281 cgcaccgcct cccggtcgcc gtcaccgagg gccgcgcaga cccacagctt gtcgtcgacg
     2341 gcgctcagcc cggagacgag accgccgccg ccgcgcttgg agtccagcgt cccgtcctcg
     2401 ccgagcgtgt acgtgaccgg gccgcggttg gacgcgacga ggacctgggc attcgaggaa
     2461 ggcgtgtgct cggagaccat gtagcggaac ctagcccgtt cgggaagccg ccaaacgcct
     2521 cggaggggca cgcgccccac ccgctcacgc cgcgtggcgc tccgcgtact cctcgatctc
     2581 ccgcatcggc ggccgctcct ccgtgtccac cgcatgggtg cgcggcacga agccgtcctc
     2641 gccgcgttcg aactgggtca gcgcgggccg caccagatgg ccacgggaga gccgcagctg
     2701 cgccgtgcgg tagatcgccg ccgccatccg gcccagcgcc tgcccgtcct gatggcggtg
     2761 gatgcgtaca ccgacgtcga cctggcccag cgcgtccagg cccaccgtgt gcagcgcgtc
     2821 caccagcaga cccagctcca ctccgtaacc gaccgggaac ggcagccgct ccagcaggga
     2881 ccgccgcacc gcgtactcgc cgcccagcgg ctgtacgaat ccggccagct gcggccagtg
     2941 cagattgagc agcggccgtg ccaccagttc ggtgacgcga ccgccctgac ctgctctgtc
     3001 gccgagcgga cggtcgtaca tcgccttgac gaactggacc gtggggtcgg tgagcagcgg
     3061 gccgatcgtg cccgagacga agtccgccga gaagtccttg aggtcggcgt ccacgaagca
     3121 gacgatctcg ccgctggtca ccaggagcga ccgccacagc acctcgccct tgccgggcag
     3181 ggccgggatc cggggcagga tcgcgtcccg gtggaccacc cgcgcgcccg ccgcccgggc
     3241 cacggcggcg gtggcgtcgg tggagccgga gtcgatcacc acgagctcgt cgaccagccg
     3301 gaccttctcc atcagctccc ggcggatcgt cgccacgatc gcgccgaccg tggcctcctc
     3361 gttcagcgcg ggcaggacca cgctcaccga cgtacggcgc ggatcggcgt cccgggcgtc
     3421 ggtgagccgg ttcagcgggc ggtcgccgga cgaccaggaa cgcctggtca gccagcgttc
     3481 cacctcttcg agcacggtcg gcactccctg tgtgtgatcc atctcgcggt tcggacgact
     3541 atctcaacgg tccgatgctt cggttacagt cttgaacaac gcgtatggcc gtcgcatgtc
     3601 ggggtcggac cgcacataaa cgcctggatc gaagatccag cgccatagag ctcatccaga
     3661 gggactgagg gaacggcccg ttgaagtccc ggcaaccctc ctgccgaccg cgaggtcacg
     3721 gtggggaagg tgccaattcc gtcttgtggc gaaatgcgtc gcaaggaaga tgaggagaaa
     3781 gggcctccgc catcatggct gttcagaccg ttgcaggtaa tgccaccacc gcccccgtgg
     3841 acctcggtcc cgccgcggcg ctttcctgcc gcgagtgcgg cgagcgtttc gagctcggcc
     3901 cccttttcgc ctgtgcgtcc tgtttcggac cgctggaagt ggcctacgac ctgcccaccg
     3961 gctccccgga ggagctgcgg aagcgcatcg aggccggtcc ggacaacatc tggcgctacg
     4021 caccgctgct gccggtgccc tccggcgtcg cggagaagcc caacatcaac cccggcttca
     4081 ccaagctggt caaggccgac aacctcgccc gtgagctggg tgtcaccggt ggcctgtacg
     4141 tcaaggacga ctccggcaac ccgacgcact ccttcaagga ccgcgtcgtc gcgatcgccg
     4201 tcgaggccgc ccgcgccttc ggtttcacca ccctctcctg ctcctccacg ggcaacctgg
     4261 ccggagccgt cggcgccgcc gccgcccgcg ccggactgcg ctcctgcgtg ttcatcccgc
     4321 acgacctgga gcagggcaag gtcgtcatgg ccgcggtgta cggcggtgaa ctggtcggca
     4381 tcgagggcaa ctacgacgac gtcaaccgct tctgctcgga gctcatcggc gacccgctcg
     4441 gcgagggctg gggcttcgtc aacgtcaacc tccgcccgta ctacggcgag ggctccaaga
     4501 cgctggcgta cgagatctgc gagcagctcg gctggcggct gcccgaccag atcgtcatcc
     4561 cgatcgcgtc cggttcccag ctcacgaaga tcgacaaggg gctgcaggag ctgatcaagc
     4621 tcggcctcgt cgaggataag ccgtacaaga tcttcggcgc ccaggccgag ggctgctccc
     4681 cggtctccgc cgccttcaag gccggtcacg acgtcgtacg gccccagaag ccgaacacca
     4741 tcgccaagtc gctggccatc ggcaacccgg ccgacggccc gtacgtcctg gacatcgccc
     4801 gccgcaccgg cggcgcggtg gaggacgtca ccgacgagca ggtcgtcgac gcgatcaagc
     4861 tgctggcccg caccgagggc atcttcggcg agacggcggg cggggtgacg ctcggcgtga
     4921 cgaagaagct catcgaggcc ggcgtcatcg acccgacgct caccaccgtc gtcgtcaaca
     4981 ccggcgacgg cctgaagacg ctcgacgcgg tctccgccac ctcgcaggcc acggccacca
     5041 tcaagccgag cctggacgcc ttccgcgccg cgggcctcgg caccgcctga cgaccacccc
     5101 gtaccgaagg aagaagggca ccaccatgag cgtcaaggtc cgcatcccca ccatcctccg
     5161 tacgtacacg ggcggccagg ccgaggtccc ggcggagggc gcgaagctct ccgaggtcat
     5221 cgagtccctg gagaaggacc acccgggcat cgccgcccgc gtcctggacg accagggcaa
     5281 gctgcgccgc ttcgtgaacg tgtacgtcaa cgacgacgac gtgcgcttcg agggcggcct
     5341 ggacgcggcc acgccggacg gcgccggcgt ctcgatcatc cccgcggtcg ccggcggttg
     5401 ctgaaccgcc ccgggccgct ccacggcggc ccccaccgca cctctgtgtg accacgattg
     5461 ccccctccgc gagagaagcg gagggggcaa ttctgcatgg ttgagcacgg tacagttggg
     5521 gaagccccat ccgctgcccg tgccgcccgc atatgagaat gcgcccggcc ccgataagaa
     5581 gcagccaaag tgcgtgaacc tcttgcggca taagtgccct ttgcctggcc cgacttgccc
     5641 gtaaatctca gcaaacccac atattcgcgt gttcttccgc gcccagattt ctcgtccgat
     5701 tgacctgttg cagagggcag ttgggcggat acattcggcc gcggtcgacg cgctgcggcg
     5761 ccgcacaccc tctcctgtcg gagggtgagt tccgacccgg gtccgcgaag tgcggtcccg
     5821 tgcaagggcc agtaataggg gagttaggca tggctcaggg caccgtcaag tggttcaacg
     5881 cggagaaggg gtacggcttc atcgcggtcg acggtggtgc ggatgttttc gtccactaca
     5941 gcgcgatcca gatggacggg taccgcaccc tcgaagaggg tcagcgagtt gaattcgaga
     6001 tctcgcaggg ccagaagggg ccccaggccg acatggtcaa gctcgccgtc ggctgagctc
     6061 ggcgcggtcg tccgacgaca ccactcacgc acgtagggcc cgaaccccgg atcagggggt
     6121 tcgggcccta cgcgcgtccg gtggcccggg cccggtccgg cgatccggtc cggtgacccg
     6181 gtcgcggggg cgcgccgccg tccacatccg acgccgccgc cgtccacatc cgacgccgcc
     6241 gctgtccaca tccgacgagg gacgcttgca ctcgcagggg tcgagtgcta atcattggcg
     6301 ttagcactct ccaggtgaga gtgacagaac ttggaccggg ccggtgaggc ccgcaggcgc
     6361 ggtggggcaa ggaaccacca cgcacgctgg ccgtccgtcg cgggcgcctc tcggtccgga
     6421 gaaatccacc cctgtccggg aggaccactt cacatggcca agatcatcgc gttcgacgag
     6481 gaggcacggc gcggtctcga gcgcgggatg aaccagctcg ccgacgccgt caaggtcacc
     6541 ctcggcccca agggccgtaa cgtcgtcctc gagaagaagt ggggcgcgcc cacgatcacc
     6601 aacgatggtg tttccatcgc caaggagatc gagctcgagg acccgtacga gaagatcggt
     6661 gcggagctgg tcaaggaggt cgccaagaag acggacgacg tcgccggcga cggtacgacc
     6721 accgccaccg ttctggctca ggctctcgtc cgcgagggtc tgcgcaacgt cgccgcaggc
     6781 gccaacccga tggccctcaa gcgcggcatc gagaaggccg tcgaggccgt ctccgccgct
     6841 ctgctggagc aggccaagga cgtggagacc aaggagcaga tcgcttcgac cgcctccatc
     6901 tccgccgccg acaccgagat cggcgccaag atcgccgagg cgatggacaa ggtcggcaag
     6961 gaaggcgtca tcaccgtcga ggagtcccag accttcggtc tggagctgga gctcaccgag
     7021 ggtatgcgct tcgacaaggg ctacatctcg gcgtacttcg ccaccgacat ggagcgtatg
     7081 gaggcgtcgc tcgacgaccc gtacatcctg atcgtcaact cgaagatcgg cagcgtcaag
     7141 gacctgatcc cgctgctgga gaaggtcatg cagtcgggca agcccctgct gatcatcgcc
     7201 gaggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnngggcga ggcgctctcc accctggtcg tcaacaagat
     7501 caagggcacg ttcaagtccg tcgccgtcaa ggctccgggc ttcggcgacc gccgcaaggc
     7561 catgctcgcg gacatcgcca tcctcaccgg tggcaccgtg atctccgagg aggtcggtct
     7621 caagctggag aacgccggcc tggacctgct cggccgcgcc cgcaaggtcg tcatcaccaa
     7681 ggacgagacg acgatcgtcg acggcgccgg tgacagcgac caggtgcagg gtcgcgtcaa
     7741 ccagatccgt gccgagatcg agaactccga ctcggactac gaccgcgaga agctccagga
     7801 gcgcctcgcg aagctggccg gcggcgtggc cgtcatcaag gccggcgccg ccaccgaggt
     7861 ggagctcaag gagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnggccggc gccgccaccg aggtggagct caaggagcgc aagcaccgca tcgaggacgc
     8161 ggtgcgcaac gccaaggccg ccgtcgagga gggcatcgtc gccggtggtg gcgtggctct
     8221 gctccaggcc tcggccgtct tcgagaagct cgacctcacg ggcgacgagg cgaccggcgc
     8281 caacgccgtg aagctcgcgc tggaggcccc gctcaagcag atcgccgtca acggtggtct
     8341 cgagggtggc gtcgtcgtgg agaaggtccg caacctgccg atcggtcacg gcctcaacgc
     8401 cgcgaccggc gagtacgtcg acatgatcgc cgagggcatt ctcgacccgg cgaaggtcac
     8461 gcgctccgct ctgcagaacg ccgcgtccat cgccgcgctc ttcctcacca ccgaggccgt
     8521 catcgccgac aagcccgaga aggccggcgc ggccgctccg ggcggcatgc cgggcggtga
     8581 catggacttc tgatcagcca cggaggcttc tgcctccccg cctgatccga agttccgcgt
     8641 acgtccccgt acgtgcgcag tacgtccgca gtacgtccca caggggcggt atctcctcac
     8701 ggaggtgccg cccctgtggc gttcccggcc gcactggaac aatgcggccg ggggccggtg
     8761 gaccggtcgt cgcgacaacg ggggacgggg acggggacgg gatgaccaag gacgggaagg
     8821 gggcctcgtt cgtgctgggc ggggccgtgc tgttcgtgct ggggctgttc gtcgtggtga
     8881 ccgggacggg gctctccggc gccctcggca tgcgggtcga gaccttcggc cggatcgact
     8941 gcgacgacac gcggacgaag aaggggcaga ccgtctggca ctgcttcggc gagagcccgg
     9001 tccagcagcg cgccaacgag gcggagcgcg aacgggtggc ccgggaggcg ctgcgcgccc
     9061 atgtggacgg cgtgccccgg ccggcggaga ccgggcgcac caggatcctc ttcgccgacc
     9121 acgacggccg tacggacccc gagaccatca ccgcgaccca ggtgttcgac ggcggacggt
     9181 ggtacgccca ctcctcgatg gtcgtcggct acggcatgat cccgctgctg ctgggcgcgg
     9241 gagcggcggc ctggggcggc taccggctgc gccgacggta ggtccgggtc cggcgggtcc
     9301 ggcgggcccg gcggacccgc tccgggcctg tggccgaatg cctacttgtc gtgtccggtg
     9361 ccgtgctgca ccgcgcgttc ctccgttttc ggcgatgtcg gtggcgcgcc ggcgacccgt
     9421 gaggtacggc cggcgcgcgg gctgccgggc cctcgcccgc catggttccc gcgttccgcc
     9481 gcacgtcgtc ggctctcttg gcagctcact gcccggtccg gccgacacgt acagccattg
     9541 gtccagtcca ctacttcgcc gcgctccggt gctgtgcgac tgcacatgcc gaccggtcgg
     9601 tgccccgcga tgccgtggag agcccgagag cggccctgcg cgccggcccc ggccctcgcg
     9661 ctatccagcc aacttcgcac ggtaagtcgg aatcatgcgt accggatcgg cagcgggaca
     9721 tgatggctct ggtgttcacg tggtgaacac cagtcgcacg aataacttca gttatgcagc
     9781 tgtcagttgg gcataacggt cgtcaaccgc acagaaggga aggtgtcatg aagcagcaga
     9841 agaaggctta cgtgaagccg tcgctgttca agcagggcga cttttcgaag aagaccgccg
     9901 gttacttcta cggctcgtac aaggagtact gggtccgccg catcatctga tccggccccc
     9961 ggtttcccgc gcgtcgtacg agaacttccg gaaggtgaac atgcccggcc aactgcgtga
    10021 ctacttcgtc gtcctgccgg acagcgtggc gggagcggcg gtggccgggc ggcttcctcc
    10081 gggggcgggc ggggggacgg tggtcgccca cgcatccggg cggccctggg tcctcgcccg
    10141 cccgctggtc cgccgggtga gccacctccg ccggggcgac gacgccctcg tgctcatcgg
    10201 cccggaccgg gtctccgacg ccgtgctctc cggtctcctg gagggggtcc gcgaccgggc
    10261 gggcctggaa cgccgcctcg cccggctgcc cggcctccac catgtgatcg cccggctctc
    10321 cgggcagacc tggatccggg gcaccgcctc cgggctgcgc cggatctacc acgcccggca
    10381 cccggaggcc ggaaccatcg cctccgaccg ccccgccgtc ctcgcccgcc tgatcggcgc
    10441 acccctggac gacggcgcac tggccctgcg catgctcgac ttcctgccgc accccctgag
    10501 ccgccgcgtc gtgtggcgcg gcgtgcacga gaccgtcgcc gggtacggcc tggccctgcc
    10561 cgccgcagca ccgggcacgg ccgccgtccc cggccccacg gagcaccgct ggtgggagcc
    10621 gccacccgcc gaactcccgc tggccgaagg ggcacaggcg ctcggcgacg ccatcgccgc
    10681 ctccgtacgg gcccatgtag acggcctgga ccggatcagc tgcgagctgt ccggcggcat
    10741 ggactccacg gccctcacct tcgccgcccg cgcgaccggc cccgccgaac tcgccctgct
    10801 caccgtcgcc gcgcgcgacc gctacagcga ggacgagacg tgggcgcgca gagcggtgga
    10861 ggcggcacga ggaggaggtg gtggggcgcg agggggcgag gcgacgcgga gtggcggggc
    10921 ggcgcgaggc tctggcggga agccagacag taacgcggcg cgtacgcgcg atacggggcg
    10981 gaagggcggc gacgtcaccg gcctggacca ccacatcatc cccgccgacg acgcccccta
    11041 cttctacgcc gatctcgcct ccaccgccgc cgagttgaac gacgaaccgc tccccgtggc
    11101 ccccggccgg gcccgcgcgc acctgctcct gagccgcgcc cacgccaccg gctcgcgcta
    11161 ccacctcacc ggctacggcg gcgacgaact cttcctcggc ctgccccacg cctaccagga
    11221 cctcttccac ggcaacccgc tcaccgcctg gaaccacctc agcggcctgc gccaccagct
    11281 cggctggccg ttcctgccga cgctgaaggc cctgctgaac cgctccacgt tcccccgctg
    11341 gctcgccggc gcgatcaccg ccgagcctca gccggtcacc cgtacaccgc tgctctcctg
    11401 gggcgtacgg cagtcgctgc gcccctggtt cacggagcac gccaacgccc tgatcgccga
    11461 ggagttccgg gccgccgccg agcacgccga gccgatcgac ccgtggcgcg gccggcacgt
    11521 cgacatcgac gccgtacgga tgggggcccg gcacttccag gcgatggagg acatcggcat
    11581 gacgatcggg ctgccggtcg ccgcgccctt ctacgacgac cgggtcctgg aggcgaccct
    11641 cgccgtacgc ctggcggagc gcatcagccc gtggcgctac aaaccgctgc tcgtcgaggc
    11701 gatgcgcggg gtggtcccgg acgcgctgct ggcccgcacg acgaaggacc acatgtcctc
    11761 cgacgaacac cagggactgc gtcaacacgc gtcggatctg gccgacttgt ggaccggctc
    11821 ccggctcgcc gagcacggcc tggtcgactc ccgccacctg ctccggctcg ccgccgaacc
    11881 cttctcaccg gtcctggtcg agcactccgt cagctccacc gtggccggcg agacctggct
    11941 ccgcaccgcc gagaacgcct ggccgacgcc gacgccgacg ccgtcacagt cgccgtcacc
    12001 gccaccccac cgcacctcca cgaccagcga ggcaaccctg tgaagctcag aaaaggcatc
    12061 gccgtcacga ccaccgagta tggcggcgtc ctcctcgacg aaaaggacgg cagctactgg
    12121 cagttgaacg acaccagcat catcgtcgtc gacaccctcg ccgccggaca cacccccgac
    12181 atcgccgtgg agcggatcct cgccgcgttc gacgtcgagc gcgccgaggc ggagtcggac
    12241 gtcgcggaac tgacccggca gctcgtcgac gcgaagatcc tgcgcccatg accaccgaga
    12301 tgacgatgcc gcgccggggc gcgggagcgg gcggggtgcg gctgcgcacg gccatcgtcg
    12361 ccgcgttcgt cctggcccgc ctgaagcccg gacggctgcg ccgggtgctg gccaggtgcg
    12421 cccggggcgc gcgcccggcc tcgtacgcgg agacgatgga ggtgctggag gcggtcgtcg
    12481 ccaccagccg ccgctgcgcg agccgttacg gctgcctgcc ccgctcggtg gccgtcgccc
    12541 tggcctgccg catgtccggc gtctggcccg actggtgcgc cggagtgcgg tcggccccac
    12601 cgttcgcccc gcacgcgtgg ctccaggcgg agggccgtac ggtcggggaa caggcggaac
    12661 cggcggatct gcgtcccttg atggtggtgg cggcagcggc ggtcacggca ggcggagggg
    12721 tacgggatga gcgcggtgtc ggggacgacg ggggcggaag cgagagccgg ggcggaggcg
    12781 gccggcagcg ggcctgagga cgcggggata ccggcggcgg ggcccgcagg tgagccggtg
    12841 gggcccgggc agaccgcagg tgaggcggca gggcccgagc ggcccaccgg ggaggcaccg
    12901 gcccccgaga cgccggccgg tgggtccccc gcggatacca ccgggaaccc cgcccagggc
    12961 aagtccggcc cgtcccccct ccgcctcctc ctcgcccgcg tccgcccgca ccggggcgta
    13021 ctgatccgcg cgggcctgct ctcgctcacc ggctccgcgg ccggcctggc gatgcccctg
    13081 atggcgaagt acgccgtcga cgcgttcgcc gccgaccgct cgcccgtggc cccgctcgtc
    13141 gcgctgaccg cgctcgtact cgccggagcc tgcatctccg cgtacgggcg ctacctcatg
    13201 gcccgtacgg gagagggcgt cgtgctccgt gcccgcgacc aactcgtggc ccgcatcatg
    13261 cggttgaagg taccggcggt ggaccgcctc acccccggcg acctgcagtc ccgggtgacc
    13321 agcgacacca ccctcctccg gacggtcctc tccagcggtc tggtcgagtc gttcaacagt
    13381 gtgctgatgc tgctgggaac ggtcgcgttc atggcgtaca tggacgtgac gctgctgggc
    13441 gtgacactgg tcgtcatcgt cggcatcgga gccgtaacgg ccttgctgat gccgcgtatt
    13501 cagcgcgcgc agctccgggc gcaggagtcc gtcggggcga tgggcgcggc cctggaccgg
    13561 gtgctccagg cgttccgtac ggtcaaggcg agcggtgccg aggagcggga gacggcagcc
    13621 gtggcggagg cggcccggca cgcgcacgac cggggcgtct cggtggcgaa atggtcctcg
    13681 gtctcggacg tcacgatgat gatgtcgatc caactcgcct tcctggccgt gctgggcgtc
    13741 ggcggagcgc gggtcgcgtc gggctcgctg gagatctcct cgctgatcgc gttcctgctc
    13801 tacctcttct acctgatggg cccgatcggc ggtctggtcg agggctggac ggggctgcag
    13861 agcggcctcg cggcggtgcg ccggatcgac gaggtggagt cgctgccggg cgagacctcc
    13921 accggatccg gggccgtgga accggagggc ggaccgctcg gggtcgcctt ccaggacgtg
    13981 accttcgggt acggggacga gcgcgccccc gcccacaagg gggtgacgtt cgacgttccg
    14041 gccggtggac tcgtcgcgct ggtgggcccg tccggggcgg gcaagtcgac ggtgttcagc
    14101 ctgctggaac gcttctacga ccacgacggc ggcacgatca ccgtgggcgg ccgggacatc
    14161 cgcgactggc cgctggccga actgcgcgcc tcgctcgcgt acgtggaaca ggacgccccg
    14221 gtcctggccg ggacgctccg cgagaacctc ctcttcgccg ccccggaggc ggaggagagg
    14281 gagctgacgg cggcggtggc ccgtacccgc ctggacgccc tggtcgaccg gctgccggac
    14341 ggcctggaca cggcggtcgg ccaccggggc gtcacgctct ccggcggcga acgccagcgg
    14401 atcgccatcg cccgcgccct gctgcgccgc ccccggctgc tgctcctcga cgaggtgacc
    14461 tcgcaactgg acgcggtcaa cgagcaggcg ctgcgcgacg tcatcctcga aatggccgag
    14521 caggcaacgg tgttggtcat cgcacaccgt ctgtccacgg tccgccacgc cgaccggatc
    14581 gtggtcctgg aggagggccg ggtccgcacg gcgggcacgc acgaggagct gatcgccggg
    14641 gacgacctgt accgcgagct ggcgacgacg cagctggcgg cggaggtggc gtgatggcgg
    14701 ggcggaggcg gcgcgatgac ggttcggcgg cgaatgccat gatgggggac aagggaaccg
    14761 gaaccggcac catcgaaagc cccgaaaccc cagcgacgcc tgcgacaccc gcaacacccg
    14821 caacacccgc gacgcccgca acgctttacg ggcccgtctc gctcggcttc ggcgtcatcg
    14881 cgctgatcgc cgcgttcttc ctcggctttt tcgccctcct ggccgggagt ctggcgatca
    14941 ccttcggtgt gctgggcctg atcagcgcgt acaaccggcc ccagtgcctc gcgggcctcg
    15001 tcctgggagc cgtgggcgtg ctctacccgc tctcgttcct cttcgccttc acgggcggct
    15061 tctgaccgcg tcagaaccga gggcggcggg tcagcgggcg tagtccttga gctcctcggt
    15121 gtccagggtg atgtcgacgg ggtgggggag ggcggcggcc ttgccgtact cgtacgtcgt
    15181 ccgctgccgg taccggccgt tctccggctc gctgtacacg acgagggcat cgtggtcgcg
    15241 gtcgatcagg aggtagaccg ggatgcctgc cgcggcgtac ccgtcacgct tctccgtgcg
    15301 atcacgatgg tccgtgtcgt ggtcgtgcga cgtgacttcg acggtcatga ggacgcggtc
    15361 cgggctcgac cactcgccgt cacccgcgaa gcgccccgcg cgggccaggg tgccgtccgg
    15421 gcgggcgcgg ccgttgcggt atccgccgat ccgcagtccg cgttcgcggt acagccgcag
    15481 ttccggctgc tgctgcatgc actgcatcgc gacccacatg atgatctcgt cgtggtcccc
    15541 gtccggcacg ggcttgacct ccagctttcc gttgatgagc tcaagcgtca cggtctcggg
    15601 ggccgtccgg gcaagctgct cgaactcctc gacggacatc tgggcgtgcg gtgcgtgatc
    15661 ggcggagctg ggggtcatgg cgtcgcctcc tcccctccat cgtgccccgt cggatctccg
    15721 ggcgagccgc acggtcatcg gtatgtcctt tcggatcgag gaacgagccc ggcccggacc
    15781 gcgttcatcc ggtcccgtac gccgggtggc tgcacggttc ccggcggccc caccggcggc
    15841 tcgggggacg gcccccggca cggccggcag aggccgccga ggagcgcttc gggcgggccg
    15901 ggcactccgc actcgccgca ttcgagggtg cgcaggacgg tccggggccc cggcaggacc
    15961 ggctcgggcg gcagcttgtc gcgcagccgc cgttcgacga ggcgggcggc gcagtggacg
    16021 ggggagggca gcccgctggt gagggcgtgc agcagctccg cctcggtcgc gccccgcgcg
    16081 atccactcac ctacctgcgg ctccaggcgt acgcagtcgg cggcggacag cgtcagggcg
    16141 ggggcggtcc gccccagggc cgccagcagg atgaacgccc gcgagcgggt gggccgttgc
    16201 ggttcgtccg gcgggacgtc gccccgggtg taggcgttcc accaggcgtc ctcgcgtgcg
    16261 gtgcgggaga agaaggtccg ggtcacccag agcatgacct cgtcggtgat cacgcactcg
    16321 ctgccgcgcc gtaagtgccc ggcgtcctgg agccggttga gggtggtgcg cagggcgcac
    16381 tgcccgtacg ggaggtgctt ggccagcgtc ttcacggaga tgtcggagcc gtcgggcagc
    16441 cggtcgatgt aggcggcgac ggaggcgtca cgggggagga ggtgggcgaa gtcgcggtcg
    16501 gtgcgcggac gttcgccggg cgcggagcgc ttgccgtagc cggggttggc catggggtgc
    16561 gggagtgtgg gcggggtctc gcgcggagcg cggggcgcat cactaaactg gctgtcagcc
    16621 atggggatcg ctttcttcgc tttctcgatc ttcggtggct agacccctgt cggtgttgga
    16681 gcaccggtag gggtcgtctt ttgcctggtc gtggtcttcc cgcacgctag agcctggcga
    16741 ccgtgcgtcg caactcgatc acaaactgtc aacttcgcag ccagggtggg cgggtgggac
    16801 tactgaccca tccaccccat cactgttaag tgcgctcgga tctcgccgct tgagcttcgg
    16861 gagatgcgtg gtgagcggcg ggggtgaggg gagggcctgg gccgttcggg tcctgtgacg
    16921 accgtcacct cggattcatg gcgggtggtg tcgaccggat ggagcgcgcc gggcgtatac
    16981 ccggtgaagc gaaagatccg ggagcaactt ttgaccgtcg aggagttcga agagttctac
    17041 gcccgggcgg tcgcgcgtct cacgggccag ctgtacgtga tgaccggcga cctccaggag
    17101 gcgcaggacg tcgtgcagga ggccttcgtc aaggcctggg tccggcgcgg ccggctggag
    17161 cgggacgggc agccggaggc gtggatccgt acggtcgcct ggcggctggc ggtgagccgt
    17221 tggcgcttcc ggcggcgttc ggcggacgcc tggggccggg agagcggcgc gcccgagcat
    17281 gtggagccgc cggacccggg ccatgtggtg ctggtcgacg cgctccggga actgcccgcc
    17341 cagcagcggc ggacgctgac cctgcactac ctgtgcgacc tcacggtcga gcagatcgcc
    17401 ggggagaccg ggctttccac cagcacgatc aagacgcatc tggtccgtgg acgggccgcg
    17461 ctcgcgcacc gtctgcacga cccccgcatc gaggaggctc ctgatgtctg aaccacggga
    17521 tccattgcgg tctctgttcc gggaggccgg tgacttcggg caggtccgct cggtggcgga
    17581 cccggcgtcg agcatcaccg aacgcggcat gcgcgcgcag cgccgccgga tggccgtggt
    17641 cgcggtgggc gtctgcctgg tcgtcggcgg gggcggggcg gtcgcggtgg gcctgctgcc
    17701 ggtacggccg gaaccggtcg ccccggcgac gagtccgtca ccgagctacc cctcgcccgt
    17761 accgtcggag ggcctgtcga ccgagcagtc gtcgccgccg ccctcgccga gcaccacgct
    17821 gccgggcggt ctgcccacgg gtacgggcac gggtgcgccc acgggcacga gtccgaccgc
    17881 gacgaagccg ccgccgacgt ccgagtcgac gacgtcgacg gcgaccgacg gagccacgtc
    17941 gacgccctga ccccgtagcc gtagccgtag tccatagccc gtaccccacc c
//
