LOCUS       NZ_KL573553            10375 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces baarnensis strain NRRL B-2842 P144_Doro1_scaffold15,
            whole genome shotgun sequence.
ACCESSION   NZ_KL573553
VERSION     NZ_KL573553.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces baarnensis
  ORGANISM  Streptomyces baarnensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 292394)
  AUTHORS   Doroghazi,J.R., Albright,J.C., Goering,A.W., Ju,K.-S., Haines,R.R.,
            Tchalukov,K.A., Labeda,D.P., Kelleher,N.L. and Metcalf,W.W.
  TITLE     A Roadmap for Natural Product Discovery Based on Large-Scale
            Genomics and Metabolomics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 292394)
  AUTHORS   Doroghazi,J.R., Ju,K.-S. and Metcalf,W.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2014) Institute for Genomic Biology, University of
            Illinois at Urbana-Champaign, 3109 IGB, MC-195, Urbana, IL 61801,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: IDBA UD v. 1.0.9
            Genome Coverage                   :: 74x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 02:19:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,395
            CDSs (total)                      :: 7,345
            Genes (coding)                    :: 7,173
            CDSs (with protein)               :: 7,173
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 55 of 173
            Pseudo Genes (incomplete)         :: 138 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 27 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 226824
            Orig. end                         :: 237199
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KL573553.1.
            Bacteria available from NRRL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10375
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10375
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10375
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5375](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5375
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(195..209)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.07"
                     /tool="antismash"
     misc_feature    266..281
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            456..1304
                     /locus_tag="BG84_RS1000000140810"
     CDS             456..1304
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /locus_tag="BG84_RS1000000140810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="WP_050495704.1"
                     /transl_table=11
                     /translation="MTTPPASAPAPSAPLLDADAAHRHRTVTRRALDRPAPGRAAAGPL
                     AAFVLTHPLAERTVAGLAAGHGPYSLVHLAQEIELHHPPRPGAPLDASAALDAVRVEAK
                     GTRLVLTTTLTDRATGAEQARLTAHILLAGIRAAEPHGTLVPTASVRQEAPGDTVSRDL
                     AIDREWIADYGHAAGDLNPVHLDPAAARAAGFADVIAHGMSLVALAVEEAAERYADGDV
                     DRVRAIGARFARPVLPGEATTLDLTPSAAGDTVAFTVRSGGAAAVKGGWLRLGPGTGAG
                     R"
     gene            1301..2800
                     /locus_tag="BG84_RS0122520"
     CDS             1301..2800
                     /NRPS_PKS="Domain: AMP-binding (15-397). E-value: 5.7e-70.
                     Score: 227.9. Matches aSDomain:
                     nrpspksdomains_BG84_RS0122520_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /locus_tag="BG84_RS0122520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AMP-binding protein"
                     /protein_id="WP_032790634.1"
                     /sec_met_domain="AMP-binding (E-value: 1.1e-64, bitscore:
                     212.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRTDRHGETAESVLRWADKTPDAPALWWNGSPTSYRELADATAA
                     ARARLAEHVPAGAPVAVLDEKSPGTVATVLACLATGRPALLPSPTLPPDHVAAVMAEAG
                     CRYALAGPAVTARDPEHGERGGGAVPPGTALVLTTSGSTGIPKAVPLPHRAVEAFTGWA
                     ADAFGIAETTPVLNYAPLNFDLCLLDVWTTLAHGGQVVLVDPATAASGRGLLRLLRTHR
                     VEVVQAVPLFYALVGTQVRVEGGGPLETVRHAAFTGDVMPEQALAELMDLAPGARLHNI
                     YGCTETNDSLIHEVDRSLPQSAPMPLGRPLPGVRLRLRNSDGRLLTGPGRGELEVSTPF
                     QSTGYADPRRAAEKFVLDPAADGGPGTRWFRTGDLVEQDEDGTLRLVGRLDHQVKIRGV
                     AVNTGEVEQVLLEHPEVLEAGVTTVPDPVEGRRLIAVVRLKDGSTANSLDLKRHCTARL
                     ARGWVPGLLVPVTEPLPRTSTGKVDRRAITRTAAAPAATAS"
     aSDomain        1346..2491
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BG84_RS0122520_AMP-binding.1"
                     /evalue="5.70E-70"
                     /label="BG84_RS0122520_AMP-binding.1"
                     /locus_tag="BG84_RS0122520"
                     /protein_end="397"
                     /protein_start="15"
                     /score="227.9"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="RWADKTPDAPALWWNGSPTSYRELADATAAARARLAEHVPAGAPV
                     AVLDEKSPGTVATVLACLATGRPALLPSPTLPPDHVAAVMAEAGCRYALAGPAVTARDP
                     EHGERGGGAVPPGTALVLTTSGSTGIPKAVPLPHRAVEAFTGWAADAFGIAETTPVLNY
                     APLNFDLCLLDVWTTLAHGGQVVLVDPATAASGRGLLRLLRTHRVEVVQAVPLFYALVG
                     TQVRVEGGGPLETVRHAAFTGDVMPEQALAELMDLAPGARLHNIYGCTETNDSLIHEVD
                     RSLPQSAPMPLGRPLPGVRLRLRNSDGRLLTGPGRGELEVSTPFQSTGYADPRRAAEKF
                     VLDPAADGGPGTRWFRTGDLVEQDEDGTLRLVGRLDHQVKIR"
     CDS_motif       1700..1759
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0122520_0001"
                     /evalue="1.70E-05"
                     /label="NRPS-A_a3"
                     /locus_tag="BG84_RS0122520"
                     /protein_end="153"
                     /protein_start="133"
                     /score="17.5"
                     /tool="antismash"
                     /translation="ALVLTTSGSTGIPKAVPLPH"
     CDS_motif       2459..2521
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0122520_0002"
                     /evalue="8.10E-08"
                     /label="NRPS-A_a8"
                     /locus_tag="BG84_RS0122520"
                     /protein_end="407"
                     /protein_start="386"
                     /score="24.6"
                     /tool="antismash"
                     /translation="VGRLDHQVKIRGVAVNTGEVE"
     gene            2896..3159
                     /locus_tag="BG84_RS0122525"
     CDS             2896..3159
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1147: putative acyl carrier protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064088.1"
                     /locus_tag="BG84_RS0122525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl carrier protein"
                     /protein_id="WP_030084126.1"
                     /sec_met_domain="PP-binding (E-value: 1.5e-06, bitscore:
                     22.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNADQISEYIVREFLPDLTPSQLPHDMDLLADGALDSLGVLKLI
                     AWVEHHFRLAVGDTDLDPENFRSVNAIDAYIARSRSTAATGG"
     gene            3163..4185
                     /locus_tag="BG84_RS0122530"
     CDS             3163..4185
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064086.1"
                     /locus_tag="BG84_RS0122530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="WP_030084129.1"
                     /transl_table=11
                     /translation="MHPALVPMVDRRAPAGPEQWDALWQALADGALDRGDALALLASLT
                     AALPEEDSLLALLDALDRRRPARPATPWPGTVNVVGTGGGPPTVNLSTAAALVAAACGV
                     RVVKSGSRAHTARTGSVDLLDRLGVPFAASLDQASGQLDAHGIVFTGPFVYPVQLARLA
                     LLAVPTPMRVFGRFLNTLGPFLAAVPVAAQVTGVSGGRVPPALRALAARPGAPATWLVT
                     NPLGADELLSICDNTVHLPDGGTLTLRAGDLVPADGTPDDLAPVPREAAAEHFRTVLAG
                     RAGGPLRATLQLNAAALALAAGHRDDWAKAVAEAGAALDDGAALDLLDRLKGKGAGCAG
                     "
     gene            4175..5014
                     /gene="trpA"
                     /locus_tag="BG84_RS0122535"
     CDS             4175..5014
                     /EC_number="4.2.1.20"
                     /GO_function="GO:0004834 - tryptophan synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064084.1"
                     /locus_tag="BG84_RS0122535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="WP_030084131.1"
                     /transl_table=11
                     /translation="MPDEFFAEARAREELGLAVFLNAGDPPLPVLTDLVAQLDASGVDC
                     LELAVPFPDSPTDGPVVRRSADRALAHGVGLRETLAFVAAVRPGLKRLRIAVLADWSHS
                     LRTAAPADYARAVADSGADALLAHGLPPRLRPAHHEALHAAGLPEVTTCYPASAPATVA
                     EAAAHATGYLYLVARYGRSGAGTPDGGHAGLAPTVAALRDLTASPIAVGFGVSTAEDLA
                     SVARSGADAAIVGSAGVAAVERSLEAGSDPVDGYRSLVAALGRPHTARPKEDTRAHP"
     gene            5001..5375
                     /locus_tag="BG84_RS0122540"
     CDS             5001..5375
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105936.1"
                     /locus_tag="BG84_RS0122540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_030084133.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.1e-31, bitscore:
                     102.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLIRNIETVKTVDWGNGLSRRFVLDADGLGYSVTDTTVRAGTKSR
                     LEYRNHLETCYCIEGSGEVVELDGTSHPLTPGVLYSLDNNDPHFLIASPHEDLRLVCVF
                     APALQGDEVHSLAEGVSSAY"
     gene            5517..6662
                     /locus_tag="BG84_RS0122545"
     CDS             5517..6662
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064082.1"
                     /locus_tag="BG84_RS0122545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_030084135.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 4e-19, bitscore:
                     63.5, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.1e-25, bitscore:
                     83.8, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.9e-29, bitscore:
                     96.9, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRNRDADQQALCAGMAQWGKILSEGHIEADARSAFEPDRWRSLAD
                     SGVLGLPFPQQWGGAGQSLLTTLHVLETLGETCRESGLNFCATTSMASTGVPLTAFGSD
                     ALRERYLPGICSGELIGAHAITEPDSGSDAMAMTTRAERDGTHYVLTGGKAFVSNGPIA
                     DVFVVYARTRPEGGPLGTTAFLVDRDTPGLSVGGTTAKMGLRTAPLSTLHLDGCRIPAD
                     RVLGRVGGGLLVLDHVMKREILFGFTVQLGEMRHRLERTVGYARERRAFGHPIGDYQAV
                     SHRVVDMKIRTDTAAKWLYDTAERLAAGEDVTADLAITKILASEAALSTSLSAVRVFGG
                     AGYLAEVGLEKEVRAAVAGTLYSGTNDIQYNHVASALGLGG"
     gene            6659..7351
                     /locus_tag="BG84_RS0122550"
     CDS             6659..7351
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105934.1"
                     /locus_tag="BG84_RS0122550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_030084136.1"
                     /transl_table=11
                     /translation="MSTPPRPLLKVCGATAPEEAAAVAPRADLVGLWYGVENGARDLTG
                     DRLTELADAVRTAGRAEPVLVTFLHDAERIAAAARAARVRWIQLHAYQPPAAVAALRAA
                     LPDAVLVKAVHVLDGACVERPFLTAYARAGADLFLVDAATRGGAVGSTGHRVPAEALAA
                     LLPVLPRPFLLAGGLGEADAPGHAFAAHPGFRGVDIDHAARADAGLLDPARVAALDRAW
                     TGTPAPAP"
     gene            7400..8212
                     /locus_tag="BG84_RS0122555"
     CDS             7400..8212
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064080.1"
                     /locus_tag="BG84_RS0122555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="WP_032790635.1"
                     /transl_table=11
                     /translation="MTQPFLSALLGADRPVIMEVKSRDAHGADLLRGRAPGDIAAAYQR
                     AGAPCVSVVTGRWFGGSAAMLHEVTARVDVPVLLKDFVTRHDQITAAARAGVSAVLLTA
                     ALLPRESLHDLARACRAHGMTPFVEITDAEEAIGLPDPQDCVVAVNNKDITARERGAGD
                     LDRSRALLPAVRAAGTLCPVSASAVGDPATAAGLLAEGYRGLLIGTALLRAPSVDAWLA
                     EFDAHRAAGTEEAGRVTVPLTVEAPPVLPHPAPVTGPAVAGEAEDVPA"
     gene            8209..9150
                     /locus_tag="BG84_RS0122560"
     CDS             8209..9150
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (52-151). E-value:
                     5.9e-09. Score: 27.8. Matches aSDomain:
                     nrpspksdomains_BG84_RS0122560_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014050076.1"
                     /locus_tag="BG84_RS0122560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_234024634.1"
                     /sec_met_domain="PF00561 (E-value: 5.6e-06, bitscore: 20.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAAPTESVATADVTAAGHDVAPGGVEQIVLGHGRHRLGAWRAEP
                     ATGAPRSVLVAVHGRAMSPGYFAGPVAPHTSLLALATSLGHTVLAVERPGYGLSRAALP
                     HGLPLAEQARLLRRSLADYAELHPVGAGFFLLGHSDGGKTALHLAGEPWTGATPADRLL
                     GLDLNGCGWHYAPAAAHFPDTLAGGAGELNWGPLRLYPGGTFRASRALLREVPAAEEAE
                     TGRWPARFPAAAARVRCPVRLTFGAYEGWWRLDRDELAAVAASFTGTRRPAVERLPEAG
                     HNLSLGLAAPLYHARALAFLEECLAASSGGRG"
     aSDomain        8365..8661
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BG84_RS0122560_Abhydrolase_1.1"
                     /evalue="5.90E-09"
                     /label="BG84_RS0122560_Abhydrolase_1.1"
                     /locus_tag="BG84_RS0122560"
                     /protein_end="151"
                     /protein_start="52"
                     /score="27.8"
                     /tool="antismash"
                     /translation="VLVAVHGRAMSPGYFAGPVAPHTSLLALATSLGHTVLAVERPGYG
                     LSRAALPHGLPLAEQARLLRRSLADYAELHPVGAGFFLLGHSDGGKTALHLAGE"
     gene            9301..9888
                     /locus_tag="BG84_RS0122565"
     CDS             9301..9888
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064078.1"
                     /locus_tag="BG84_RS0122565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1772 domain-containing protein"
                     /protein_id="WP_030084141.1"
                     /transl_table=11
                     /translation="MSHYPPHATPYQAGPVRPGGHPPAGDRITAPLLVASVLCTGLMAG
                     LFFAYDISVMPGLAELDDAAYAAAMQRFNAAIDGSALFGLVFLATLGLTVAAAIVAFRR
                     KRRAVALPLAAAALCYLLVLVVTVAVSLPLNADLAALGGSASAQDVHAVIDDFKSVWVP
                     VNIFRTLFCVLSLGALCAALLRYGKPETPVLR"
ORIGIN
        1 gccggatacg ccgccacctc ggacgcccac cacctctcgg ccccgcaccc cgacggggcg
       61 tacgccgccg aagccatgac cggcgcgctc gccgacgccg gtctcgcccc ctcggacatc
      121 gcccacgtca acgcccacgg caccgcaacc gtgcacaacg accgcgccga agccaccgcc
      181 ctggccaagg tgttcgggcc ggacggcccg ccggtcaccg ccagcaaggg cgtgctgggc
      241 cacctcatcg gagcggccgg agccgtcgaa ctcgtggtcg ccgtgctgtc gatgaacgcc
      301 ggtgcggtgc cgcccaccgc caaccacaca cgaacggaac ccgagatgga gatcgacgtg
      361 gtccacggca ccccgcggcc tgtccccacc ggccccgcgc tgaccaactc cttcggcttc
      421 ggcggccaca acagcagcct ggtggtgacc cccgcatgac gaccccgccc gcctccgcgc
      481 ccgccccgtc cgcaccgctc ctcgacgcgg acgccgccca ccgccaccgc accgtcaccc
      541 gccgggcgct ggaccgcccg gcacccggcc gggccgcggc ggggcccctc gccgccttcg
      601 tcctcaccca tccgctcgcc gagcgcacgg tcgccggcct cgccgccggc cacggcccgt
      661 actcgctggt ccacctcgcc caggagatcg agctgcacca cccgccgcgc cccggcgccc
      721 cgctcgacgc gtccgccgca ctcgacgccg tacgcgtcga ggccaagggc acccggctcg
      781 tcctgaccac cacgctcacc gaccgggcga ccggcgccga acaggcccgg ctcaccgccc
      841 acatcctgct cgccggcatc cgagcggccg aaccgcacgg caccctcgtc cccacagcat
      901 cggtgcgtca ggaggcgccg ggcgacacgg tcagccgcga cctcgcgatc gaccgcgagt
      961 ggatagccga ctacgggcac gcggccggcg acctcaaccc cgtccacctc gacccggcgg
     1021 ccgcccgggc ggccgggttc gccgatgtca tcgcccacgg catgagcctc gtggcacttg
     1081 ccgtggagga ggccgccgaa cggtacgcgg acggagacgt cgaccgggtc cgggcgatcg
     1141 gcgcgcgctt cgcccgcccg gtcctgcccg gcgaggcaac gaccctcgac ctcacgccct
     1201 ccgccgccgg ggacaccgtc gccttcaccg tacggtccgg gggcgccgcg gccgtcaagg
     1261 gcggctggct ccggctgggc cccgggacgg gagcgggccg atgacccgga ccgaccgcca
     1321 cggcgagacc gccgaatccg tactccgctg ggccgacaag accccggacg cccccgccct
     1381 gtggtggaac ggcagcccca ccagctaccg ggaactggcc gacgcgaccg ccgccgcccg
     1441 cgcccggctc gccgaacacg tccccgccgg ggccccggtc gccgtgctcg acgagaagtc
     1501 gcccggcacc gtcgccaccg tcctggcctg cctggccacc ggccgccccg cactgctgcc
     1561 ctcgcccacc ctgccgcccg accacgtcgc ggcggtgatg gcggaggcgg gctgccggta
     1621 cgcgctcgcc ggtcctgccg tcacggcgag ggatccggag cacggcgagc gcggcggggg
     1681 agcggtgccg ccgggcaccg ccctcgtcct caccacctcc ggctccaccg gcatccccaa
     1741 ggcggtcccg ctgccgcacc gggccgtgga ggccttcacc ggctgggccg cggacgcctt
     1801 cggcatcgcc gagaccaccc cggtgctcaa ctacgccccg ctcaacttcg acctctgcct
     1861 gctcgacgtc tggaccaccc tcgcccacgg cggccaggtg gtcctggtgg acccggcgac
     1921 ggcggccagc ggccgggggc tcctccgcct gctgcgcacc caccgcgtcg aggtggtcca
     1981 ggcggtcccg ctcttctacg ccctggtcgg cacccaggtc cgggtggagg gcggcggccc
     2041 gctggagacc gtccggcacg cggcgttcac cggcgacgtg atgcccgagc aggcgctcgc
     2101 cgaactcatg gacctcgccc ccggcgcccg gctgcacaac atctacggct gcaccgagac
     2161 caacgacagc ctgatccacg aggtcgaccg gagcctcccg cagtccgccc cgatgccgct
     2221 gggccgcccg ctccccggcg tacgcctgag gctgcggaac tccgacggcc ggctgctgac
     2281 cgggcccgga cgcggcgaac tggaggtctc cacccccttc cagagcaccg gttacgccga
     2341 tccgcgccgg gccgccgaga agttcgtcct ggacccggcg gcggacggcg gtccgggcac
     2401 ccggtggttc cgcaccggcg acctggtcga gcaggacgag gacggcactc tccggctcgt
     2461 cggccggctg gaccaccagg tgaagatccg cggcgtcgcc gtcaacaccg gcgaggtcga
     2521 acaggtgctg ctggaacacc ccgaggtgct ggaggccggg gtgaccaccg tccccgaccc
     2581 ggtcgagggc cgccgtctga tcgccgtcgt acggctgaag gacggctcca ccgccaacag
     2641 cctcgacctc aaacggcact gcaccgcccg gctggcccgc ggctgggtac cgggcctcct
     2701 cgtccccgtg accgagcccc tgccgcgcac cagcaccggc aaggtggacc gccgcgcgat
     2761 cacgcggacc gcggccgcac ccgccgccac cgcgtcctga cccggaccga ccgtcctccg
     2821 cggacccccg gcagccgccg gcgggccccg cgccacccga gaaccccgta cgagcaaccc
     2881 gccaaggagc accccgtgag caacgccgac cagatcagcg agtacatcgt ccgggagttc
     2941 cttcccgacc tcaccccctc ccaactcccg cacgacatgg acctcctggc cgacggcgcg
     3001 ctcgacagcc tcggcgtcct caagctgatc gcctgggtcg agcaccactt ccggctggcg
     3061 gtcggcgaca ccgacctcga ccccgagaac ttccgcagcg tcaacgccat cgacgcctac
     3121 atcgcgcgct cccggagcac cgccgccacg ggaggctgag ccgtgcaccc ggccctcgtc
     3181 ccgatggtcg accggcgcgc cccggccggc cccgagcagt gggacgcact ctggcaggcg
     3241 ctggccgacg gagcactgga ccgcggtgac gcgctggcgc tcctcgcctc cctcaccgcg
     3301 gccctgcccg aggaggacag cctgctcgcc ctgctcgacg cgctggaccg gcggcggccc
     3361 gcgcggcccg cgacgccctg gcccggcacc gtgaacgtgg tgggcaccgg cggcggtccg
     3421 cccaccgtga acctgtccac ggccgccgcg ctggtcgccg ccgcctgcgg ggtccgggtc
     3481 gtcaagtccg gctcccgcgc ccacaccgcg cgcaccggtt cggtcgacct cctggaccgg
     3541 ctgggcgtcc cgttcgccgc ctcactcgac caggcgtccg gccagctgga cgcccacggc
     3601 atcgtcttca ccgggccgtt cgtctacccc gtgcagctgg cccggctcgc cctgctggcc
     3661 gtgcccacgc ccatgcgggt gttcggccgg ttcctcaaca ccctgggccc gttcctcgcg
     3721 gccgtcccgg tggccgccca ggtcaccggc gtcagcgggg gccgcgtacc gcccgccctg
     3781 cgggccctgg ccgcccggcc cggcgccccg gcgacctggc tggtgaccaa cccgctcggc
     3841 gccgacgaac tcctcagcat ctgcgacaac acggtccacc tccccgacgg cggcaccctg
     3901 acactccgcg ccggcgacct cgtccccgcg gacggaacac ccgacgatct cgcccccgtc
     3961 ccccgggagg ccgccgccga gcacttccgt accgtcctcg ccggccgggc gggcggtccc
     4021 ctgcgcgcca ccctccagct caacgcggcc gcgctcgccc tggcggccgg tcaccgcgac
     4081 gactgggcga aggcggtcgc cgaggcgggc gccgcgctcg acgacggggc tgcgctcgac
     4141 ctgctggacc ggctgaaggg gaagggtgcg ggctgtgccg gatgaattct tcgccgaggc
     4201 ccgtgcccgc gaggagctgg gccttgcggt cttcctcaac gcgggcgacc cgccgctgcc
     4261 cgtcctcacc gacctcgtcg cgcagctgga cgcctcgggc gtcgactgcc tcgaactggc
     4321 cgtccccttc cccgactcgc ccaccgacgg gcccgtcgtg cgccggtccg ccgaccgtgc
     4381 gctggcgcac ggcgtcgggc tgcgcgagac cctggccttc gtcgccgccg tacggccggg
     4441 gctgaagcgg ctgcggatcg cggtgctcgc cgactggagc cacagcctgc gcaccgccgc
     4501 gcccgccgac tacgcccgtg ccgtcgcgga cagcggagcc gacgccctgc tggcccacgg
     4561 cctgccgccc cggctgcggc cggcccacca cgaggcgctg cacgcggcgg gcctgcccga
     4621 ggtcaccacc tgctatccgg ccagcgcacc ggccaccgtc gccgaggcgg cggcccacgc
     4681 caccggatac ctctacctgg tcgcgcgcta cgggcgcagc ggcgccggaa cgcccgacgg
     4741 cggccacgcc ggtctggcgc cgaccgtcgc cgccctgcgc gacctcaccg cctccccgat
     4801 agccgtcggc ttcggggtgt ccaccgccga ggatctcgcc tccgtcgccc gcagcggcgc
     4861 ggacgcggcg atcgtcggct ccgccggagt ggccgcggtg gaacggagtc tggaggccgg
     4921 gtccgacccg gtcgacggct accgctccct cgtcgccgcc ctcggccgtc cccacaccgc
     4981 ccgtccgaag gaggacaccc gtgctcatcc gtaacatcga gaccgtcaag accgtcgact
     5041 ggggcaacgg actcagccgc agattcgtcc tggacgccga cggcctgggc tacagcgtca
     5101 ccgacaccac cgtgcgggcc gggaccaagt cccgcctgga gtaccgcaac cacctggaga
     5161 cctgctactg catcgagggc tccggcgagg tggtggaact cgacggcacc agtcatccgc
     5221 tgacgccggg cgtcctctac tcgctcgaca acaacgaccc gcacttcctg atcgcctcgc
     5281 cgcacgagga cctgcgtctg gtctgcgtct tcgccccggc gctccagggc gacgaggtgc
     5341 acagcctggc cgagggcgtc tcctcggcct actaggcctg tccgggccgc acagccgctc
     5401 acccgacggg cgcctgaacc acgggcgccc gccgatccga cgacgggaca ccgccgtcac
     5461 ctctccgccc gtcatcgacc gaggccaccc gcacggcccg tacggaaggc tccgccatga
     5521 gaaaccgcga cgcggatcag caggcgctct gcgccggcat ggcccagtgg ggcaagatcc
     5581 tgagcgaagg acacatcgag gcggacgccc ggtccgcgtt cgaaccggac cgctggcggt
     5641 ccctcgccga cagcggagtc ctcggcctgc cgttccccca acagtggggc ggcgcgggcc
     5701 agtccctcct gaccaccctg cacgtcctgg agaccctcgg cgagacctgc cgggagtccg
     5761 ggctgaactt ctgcgccacc acgtcgatgg ccagcaccgg cgtccccctg accgccttcg
     5821 gcagcgacgc cctgcgcgag cgctatctgc ccggcatctg ctcgggcgag ctgatcggcg
     5881 cgcacgccat caccgagccc gacagcggat ccgacgccat ggcgatgacc acccgtgccg
     5941 agcgcgacgg cacgcactac gtcctcaccg gcggcaaggc cttcgtcagc aacggcccca
     6001 tcgccgacgt cttcgtcgtc tacgcgcgta cccgaccgga gggcggaccg ctcggcacca
     6061 ccgccttcct cgtcgaccgg gacacccccg ggctctccgt cggcgggacg accgccaaga
     6121 tgggcctgcg caccgccccg ttgtccaccc tccacctcga cggctgccgg atacccgccg
     6181 accgggtgct gggccgggtc ggcggcggac tgctcgtcct ggaccatgtg atgaagcgcg
     6241 agatcctgtt cggcttcacc gtccagctcg gcgagatgcg ccaccggctg gagcgcaccg
     6301 tcggctacgc ccgggaacgc cgggccttcg gccaccccat cggcgactac caggcggtct
     6361 cgcaccgcgt ggtcgacatg aagatccgca ccgacaccgc cgccaagtgg ctctacgaca
     6421 ccgccgagcg gctcgccgcg ggcgaggacg tcaccgccga cctcgccatc accaagatcc
     6481 tcgccagcga ggccgccctg tccaccagcc tctcggcggt ccgcgtcttc ggcggcgcgg
     6541 gctacctcgc cgaggtcggt ctggagaagg aggtacgcgc cgcggtcgcg ggcaccctct
     6601 actccgggac caacgacatc cagtacaacc acgtcgcctc ggccctgggg ctgggcggat
     6661 gagcaccccg ccccggccgc tgctcaaggt gtgcggcgcc accgcccccg aggaggccgc
     6721 cgccgtggcg ccacgggccg atctcgtcgg cctctggtac ggggtggaga acggggcccg
     6781 cgatctgacc ggcgatcgac tgaccgaact ggccgacgcc gtccgcacgg cgggccgggc
     6841 cgagccggtg ctcgtcacct tcctgcacga cgccgaacgg atcgccgcgg ccgcccgcgc
     6901 cgcccgggtg cgctggatcc agctccacgc ctaccagccg cccgcggccg tggccgccct
     6961 gcgcgcggcc cttcccgacg cggtgctggt caaggccgtg cacgttcttg acggggcgtg
     7021 cgtcgagcgg ccgttcctga cggcgtacgc ccgcgccgga gcggacctgt tcctcgtcga
     7081 cgcggcgacc cggggcgggg cggtcggcag taccggccac cgcgtgccgg ccgaggcgct
     7141 ggcggccctg ctgcccgtcc tgccccggcc gttcctcctc gcgggcggcc tcggcgaggc
     7201 cgacgcaccg ggtcacgcct tcgccgcgca cccggggttc cggggcgtcg acatcgacca
     7261 cgccgcccgt gcggacgccg gcctcctcga cccggcccgg gtcgccgcgc tggaccgcgc
     7321 ctggaccggc acacccgccc ccgcgccgtg acgtcccccg ccccgcaaga gaagcccgcc
     7381 acttcgtgag gaaagcccga tgacgcagcc cttcctctcc gcgctcctcg gcgcggaccg
     7441 tcccgtgatc atggaggtga agtcccgcga cgcgcacggc gccgacctgc tgcggggccg
     7501 cgcccccggc gacatcgccg ccgcctacca gcgggccgga gccccgtgcg tgtccgtcgt
     7561 gaccggccgc tggttcggcg gttcggccgc catgctgcac gaggtgacgg cccgcgtgga
     7621 cgtcccggtg ctgctcaagg acttcgtgac ccggcacgac cagatcaccg ccgccgcccg
     7681 agcgggcgtc tcggcggtcc tgctgaccgc ggcgctgctg ccccgggagt ccctgcacga
     7741 cctggcccgc gcatgccggg cgcacgggat gaccccgttc gtggagatca ccgacgccga
     7801 ggaggcgatc ggtctgccgg acccgcagga ctgcgtcgtc gccgtcaaca acaaggacat
     7861 caccgctcgc gaacggggtg cgggagacct ggaccgcagc cgtgcgctgc tgcccgccgt
     7921 acgggccgcg ggcacgctct gcccggtcag cgccagcgcg gtgggcgacc ccgccaccgc
     7981 cgccggtctg ctggccgagg gctaccgggg cctgctcatc gggaccgcgc tgctgcgggc
     8041 gccgagcgtg gacgcctggc tggccgagtt cgacgcacac cgcgccgcgg gcaccgagga
     8101 ggccgggcgg gtcacggtgc cgctcaccgt cgaagcgccc ccggtcctgc cgcaccccgc
     8161 ccccgtgacc ggccccgcgg tcgcgggcga ggccgaggac gtgcccgcgt gagcgccgcg
     8221 ccgacggagt cggtggccac ggcggacgtc accgcggccg ggcacgacgt cgctccgggc
     8281 ggggtggaac agatcgtgct gggccacggc cgccaccgcc tcggcgcctg gcgggccgaa
     8341 ccggccaccg gcgcgcccag gtcggtactc gtcgccgtcc acggccgggc catgtcaccc
     8401 ggctatttcg ccggaccggt cgccccgcac acctcgctcc tcgccctcgc cacctcgctc
     8461 ggccacacgg tcctcgcggt ggaacggccc ggctacggtc tctcccgcgc cgccctcccg
     8521 cacggtctgc cgctcgccga acaggcccgg ctgctgcgcc gatcgctcgc cgactacgcc
     8581 gaactccacc cggtcggggc cgggttcttc ctgctcggac actccgacgg cggcaagacc
     8641 gccctgcacc tggcagggga gccctggacc ggagcgaccc ccgccgaccg cctgctcggg
     8701 ctcgacctca acggctgcgg ctggcactac gcaccggccg ccgcccactt cccggacacg
     8761 ctggccggcg gggcgggaga gctcaactgg gggccgctgc gcctctatcc gggcggcacc
     8821 ttccgggcca gccgtgccct cctccgtgag gttccggccg cggaggaggc ggagacgggg
     8881 cggtggcccg cccggttccc ggcggccgcc gcccgggtgc gctgcccggt acgcctcacc
     8941 ttcggcgcgt acgagggctg gtggcgcctg gaccgggacg aactggccgc ggtggccgcg
     9001 agcttcacgg gcacgcgccg cccggccgtc gaacgcctgc cggaggccgg acacaacctc
     9061 agcctcgggc tggccgcccc gctctaccac gcacgggcgc tggcgttcct ggaggagtgc
     9121 ctggcggcct cctccggcgg ccgggggtga cgtacgcgta tgcgcgtgag tgcgtgaggt
     9181 atggaccttt gggccctgaa cctgttggga tgatcgcgcg caccgcgtac acgctccgtt
     9241 ctgccgcgtg caccgtacgc caccgcccgg gagagtccat ccgatcgaca ggactgcgac
     9301 atgagccatt accctcccca tgccaccccg taccaggccg gcccggtccg gcccggtggg
     9361 catccgcccg ccggtgaccg gatcaccgcg ccgctcctcg tggcatccgt gctctgcacg
     9421 gggctgatgg cggggctgtt cttcgcctac gacatctcgg tgatgccggg cctggccgag
     9481 ctggacgacg ccgcctacgc ggcggccatg cagcggttca acgcggcgat cgacggcagc
     9541 gcgctgttcg gcctggtctt cctcgccacg ctggggctca cggtcgccgc cgcgatcgtg
     9601 gccttccgcc gcaagcgccg cgcggtggcc ctgccgctgg ccgccgcggc actctgctat
     9661 ctgctggtgc tcgtcgtcac cgtcgcggtc agccttcccc tcaacgcgga cctcgcggcg
     9721 ctcggcggct ccgcgtccgc ccaggacgtg cacgcggtga tcgacgactt caaatcggtc
     9781 tgggtgccgg tgaacatctt ccggaccctt ttctgtgttc tgagtctcgg tgctctgtgc
     9841 gccgctctgc tccggtacgg aaagccggaa acccctgtgc tccggtaggg aaaagcccgg
     9901 agaggcaatt ccccgggctt cgttccgtat cggcacgccc ctgccttccg tattctcgcg
     9961 tgcccatcct tcatttgcgt tcgcataggg cgagccctct ttctgcgccg gtcggcgaaa
    10021 gggggccacc tgtgcgtgcc gtgctccgcc tccgtaccgc ccgacgcggt gcggccgctc
    10081 tccggccccc gccggtcggc ggcccggaga gatgtgggag cgtgggaacg gtgagtgaca
    10141 atccgacgga atccatgcag taccgcttcg acgggcccga ggacgccccg gtcctggtga
    10201 tcggcccttc gctcgggacg acgtggcaca tgtgggatcg ccagattccc gagctgtccc
    10261 agcactggcg gatcttccgc tacgacctgc ccggccacgg cggcgcgccc gcccacgccg
    10321 ccgcctccgt cgccgagctg ggcgaccggc tgctcgcgac cctggacggt ctcgg
//
