LOCUS       NZ_KL573547            10945 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces baarnensis strain NRRL B-2842 P144_Doro1_scaffold9,
            whole genome shotgun sequence.
ACCESSION   NZ_KL573547
VERSION     NZ_KL573547.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces baarnensis
  ORGANISM  Streptomyces baarnensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 194689)
  AUTHORS   Doroghazi,J.R., Albright,J.C., Goering,A.W., Ju,K.-S., Haines,R.R.,
            Tchalukov,K.A., Labeda,D.P., Kelleher,N.L. and Metcalf,W.W.
  TITLE     A Roadmap for Natural Product Discovery Based on Large-Scale
            Genomics and Metabolomics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 194689)
  AUTHORS   Doroghazi,J.R., Ju,K.-S. and Metcalf,W.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2014) Institute for Genomic Biology, University of
            Illinois at Urbana-Champaign, 3109 IGB, MC-195, Urbana, IL 61801,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: IDBA UD v. 1.0.9
            Genome Coverage                   :: 74x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 02:19:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,395
            CDSs (total)                      :: 7,345
            Genes (coding)                    :: 7,173
            CDSs (with protein)               :: 7,173
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 55 of 173
            Pseudo Genes (incomplete)         :: 138 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 27 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 124768
            Orig. end                         :: 135713
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KL573547.1.
            Bacteria available from NRRL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10945
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10945
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10945
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5945](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5945
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(607..1281)
                     /locus_tag="BG84_RS0117280"
     CDS             complement(607..1281)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971207.1"
                     /locus_tag="BG84_RS0117280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_030082193.1"
                     /transl_table=11
                     /translation="MRVLLVEDDDDLREVIASGLRDGGFAVDCAADWPEADVLLHVTAY
                     DCVVLDRMVPSGDTLVPLEVRRRAGWSVPVLCLTALDSLPERLRGLESGADDYLAKPFA
                     MPELVLRVRGLSRRAAARLPSFLSCADVVMDVARYEVRRGGVLLSLSPKEYAVLQQLLV
                     HRDAVVTRTDLLEHCWDEMADPVSNVVDAVVAGLRRKLGSPGLVHTVRGQGFLLSAEPG
                     PS"
     gene            complement(1364..2434)
                     /locus_tag="BG84_RS0117285"
     CDS             complement(1364..2434)
                     /GO_function="GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511040.1"
                     /locus_tag="BG84_RS0117285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methyltransferase"
                     /protein_id="WP_030082195.1"
                     /transl_table=11
                     /translation="MTGSQSQEAGNGRETAVDWQARGTVARLVLGQMATQALGTAVRLG
                     VFDRIGPGELSGDTLAGSLGTHPQATLRLLRALAGLQLLSETEPGVFRTTTAGDALRTD
                     TPGTMVAMARMFTDPVMLRGWDLLDESVRTGETTFDTVFGTDFFGHLKEQPELSAAFNE
                     AMSQGTRLTASNVPHHYDFGRFRTLVDIGGGDGTLLASVLRAHPELRGVLFDTAEGLAQ
                     APRRLAQQDVTGRVVLETGDFFASAPAGGDLYLLKSISHDWDDERCAAILRRIRAVIPD
                     HGSLLIVEPVLPATVPADPAGGVYLSDLNMLVNVGGRERTEDDFAALCAAGGFALRSVT
                     PLPAPDIFRIIEASPV"
     misc_feature    2451..2469
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.1"
                     /tool="antismash"
     misc_feature    complement(2456..2474)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.4"
                     /tool="antismash"
     gene            complement(2533..3465)
                     /locus_tag="BG84_RS0117290"
     CDS             complement(2533..3465)
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971209.1"
                     /locus_tag="BG84_RS0117290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_030082197.1"
                     /transl_table=11
                     /translation="MMFELPLDQVRTLLAVVDEGTFDAAAGVLQLTPSAVSQRVKALEQ
                     RTGRVLLMRTKPVRPTESGEVVVRFARQLSRLERDARTALGMAGPDGPTPLSIAVNSDS
                     LATWFLPALRRVPEELDLCYELRREDQDHTAALLREGLVMAAVTSSPEAVTGCSVRRLG
                     RMRYLPVASPAFADRWLERRAGTALHELIGDAPVIGFDRRDDLQDAFVRRLGLGPRASA
                     RRHLVPTSEGFVNAVAAGMGWGMVPEVQAEPLLGDGRLVRLAPDDAPGRSVVVPLYWQQ
                     WKLDSPALAAVADAVAAEAAEALDGDRPD"
     gene            3533..4144
                     /locus_tag="BG84_RS0117295"
     CDS             3533..4144
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="BG84_RS0117295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysE family transporter"
                     /protein_id="WP_030082199.1"
                     /transl_table=11
                     /translation="MMNALTAAAAGFGTGLSLIVAIGAQNALVLRQGVRRDGVLAVVGI
                     CALSDAVLITLGVAGVGAVVVAWPSALTVVGLLGGLFLIGYGLLAARRVLRPSALYAAD
                     APATSRRGVVLTCLAMTWLNPHVYLDTVFLLGSVAADRGPLRWTFGLGAVLASLCWFVA
                     LGYGARLLSGVLARPAAWRVLDALVAVMMIGLGAKLALDA"
     gene            complement(4206..4844)
                     /locus_tag="BG84_RS0117300"
     CDS             complement(4206..4844)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060641.1"
                     /locus_tag="BG84_RS0117300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_030082200.1"
                     /transl_table=11
                     /translation="MAKQDRAIRTRQTILMAAAQAFEKYGYQAATITEILKSSGVTKGA
                     LYFHFPSKEDLALGVLDAQEPPQTVPDQALKLQELIDLGMLFSHRLQTSLLARAGVRLS
                     MDQQAQGLDRSGPFLRWQEATHHLLTRAKHNGELLPHVNPTETADLYVAAFAGTQAVSQ
                     TLTNYQDLQRRHITLQQHILPSIATPSILTALDLTPERAERLGLLDPVG"
     gene            5001..5945
                     /locus_tag="BG84_RS0117305"
     CDS             5001..5945
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971211.1"
                     /locus_tag="BG84_RS0117305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_030082201.1"
                     /sec_met_domain="AfsA (E-value: 2.7e-26, bitscore: 85.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDAAVLIDPVPAVESEAEVVHPVGIDMVHRSRPEDAFPRHWVRL
                     ARDRFSVAAVLPHDHPFFAPVSGDRHDPLLVAEAMRQAAMLSFHAGYGVPVGYHFLMAD
                     LDYACDLEHLGVGGAPAEIDVEVFCSDLKWRGGLPVQGRVGWAVHRDGELAATGTGATR
                     FTNPKVYRRMRGDTPAEGISIPRATPVSTAGAGRTRAEDVVLSEAGRDGVWELRVDTRH
                     PTLFQRPNDHVPGMLLLEAARQAACLAVGPGGIVPAEARSRFHRYAEFDSPCSIGAVVR
                     PGAAEDTVTVQVTGHQDGELVFSTVLSGPRVNG"
     gene            5957..6802
                     /locus_tag="BG84_RS0117310"
     CDS             5957..6802
                     /NRPS_PKS="Domain: PKS_KR (1-126). E-value: 1.2e-06. Score:
                     20.5. Matches aSDomain:
                     nrpspksdomains_BG84_RS0117310_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060637.1"
                     /locus_tag="BG84_RS0117310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_030082202.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGREVAGQLEAAGPVRILARSPERLTVRGSGVEVV
                     EGEYGDRSALDRAMRGVSSVFLVTKSPTEPHDEWVAAAAAAAGVRHLVKLSMMAVEEPG
                     ADDFITRRQRENEQAVRESGVAWTFVRPRTFMSNTLSWAPGIRSAGVVRALYGDAPVAC
                     VDPRDVAAVAVTALTGTGHEGRAYAVSGPEAITAREQTAELSRVLERPLRFEELGVDAA
                     RTALLARYPRPVAEAFLESAERQRAGAKAAVVPTVEELTGRPARPFRIWSADHADAFSA
                     "
     aSDomain        5960..6334
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BG84_RS0117310_PKS_KR.1"
                     /evalue="1.20E-06"
                     /label="BG84_RS0117310_PKS_KR.1"
                     /locus_tag="BG84_RS0117310"
                     /protein_end="126"
                     /protein_start="1"
                     /score="20.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="ILVTGATGAVGREVAGQLEAAGPVRILARSPERLTVRGSGVEVVE
                     GEYGDRSALDRAMRGVSSVFLVTKSPTEPHDEWVAAAAAAAGVRHLVKLSMMAVEEPGA
                     DDFITRRQRENEQAVRESGVA"
     CDS_motif       5963..6016
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BG84_RS0117310_0001"
                     /evalue="3.90E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="BG84_RS0117310"
                     /protein_end="20"
                     /protein_start="2"
                     /score="13.1"
                     /tool="antismash"
                     /translation="LVTGATGAVGREVAGQLE"
     gene            7070..7798
                     /locus_tag="BG84_RS0117315"
     CDS             7070..7798
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515159.1"
                     /locus_tag="BG84_RS0117315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_078611174.1"
                     /transl_table=11
                     /translation="MTKQERATRTRRSLIRSAAVVFEQHGYAQARLTLISSGAGVSTGA
                     LHFHFENKAAVADAVVVEASRELREMSGAIRRRTDTALQALVDSSHALAERLREDPVSR
                     AGFRLSCDAAGTAAPDLRVQWHHRVRELLDDAAAAGTLADDVSREDAAAATVAATAGFE
                     VLGRHDPGWLSPYTLTGFWQLLLPRLAAAETLPLLDPAGTGATSSPRIPAPAAGATDEA
                     EAAAGVASGEVASAAVAEAD"
     gene            7867..8610
                     /locus_tag="BG84_RS0117320"
     CDS             7867..8610
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060632.1"
                     /locus_tag="BG84_RS0117320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_030082204.1"
                     /transl_table=11
                     /translation="MADEMFVDPRLAALYDALDPDRGDLDPYVRIAEEFGARRVLDIGC
                     GTGVFALLLADRGFEVVGVDPARASVDVARAKPGGDRVRWISGDASALPPLRVDLVTMT
                     ANVAQEITGPRDWRATLRGAYEALRPGGHLVLETRNPARRAWEEWNRERSHRVTEIAGL
                     GPVERWVEVTEVAGPLVSFRWTYVFAADGQVLTSDSTLRFRERQEAEADLTAQGFVVRE
                     VRDAPDRPGREFVLIARRPDPAPPR"
     gene            complement(8573..9502)
                     /locus_tag="BG84_RS0117325"
     CDS             complement(8573..9502)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459219.1"
                     /locus_tag="BG84_RS0117325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_030082205.1"
                     /transl_table=11
                     /translation="MDDQPEAAAPEPLDRRAELSEFLRTRRAKLQPQDVGLPEFGRARR
                     VPGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLTDAEHAHLNHLARP
                     ARHKKKRRPAKVQRVRTGLLYLLDNMEGIPAYVAGARSDILAWNPMAAAVFGDWAALPP
                     AERNWARLVFLSPAYRDLFLNWDSKASDMVSYLRLYAGCHPDDPQLSALVGELSLKSEE
                     FRRLWATHNVKEKGHGIKLIRHPLVGDLTLSYETLNLPDDEDQQLVTYHAEPGSESAQA
                     LRLLASWGADAGRADVISGAGRGPDDER"
     gene            9665..10705
                     /locus_tag="BG84_RS0117330"
     CDS             9665..10705
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-269). E-value: 5.5e-13.
                     Score: 40.6. Matches aSDomain:
                     nrpspksdomains_BG84_RS0117330_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="BG84_RS0117330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_030082206.1"
                     /sec_met_domain="ADH_N (E-value: 2.4e-29, bitscore: 95.0,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1e-24, bitscore:
                     80.5, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLERTTIERRPVGEFDILIDIKFAGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVAETGSGVTRFKVGDRVGVGCMVDSCGTCDACLMGR
                     EQHCAEGNTQTYNALDRSGEPTYGGYSTHIVVTEKFAVTIPEGIALDEAAPLLCAGITT
                     YSPLKRWGAGPGKKVAVVGLGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHY
                     YATSDPKTFEELAGTFDVILSTVSAPLDFGAYLGLLRTDGTLVNVGAPEEPVSLNLFSL
                     IMGNRSIAGSAIGGIAETQEMLDFCAVHGLGAEIEVIGASQVNEAYERVLASDVRYRFV
                     IDTATI"
     aSDomain        9722..10471
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BG84_RS0117330_PKS_ER.1"
                     /evalue="5.50E-13"
                     /label="BG84_RS0117330_PKS_ER.1"
                     /locus_tag="BG84_RS0117330"
                     /protein_end="269"
                     /protein_start="19"
                     /score="40.6"
                     /tool="antismash"
                     /translation="TIERRPVGEFDILIDIKFAGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVAETGSGVTRFKVGDRVGVGCMVDSCGTCDACLMGREQHCAEGNTQTYNALDRSG
                     EPTYGGYSTHIVVTEKFAVTIPEGIALDEAAPLLCAGITTYSPLKRWGAGPGKKVAVVG
                     LGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHYYATSDPKTFEELAGTFDVI
                     LSTVSAPLDFGAYLGLLRTDGTLVNVGA"
ORIGIN
        1 catgcccggt cagcgcggcc agcgccgtca gcgccccggt cccgacgacg agggagagga
       61 ccagactgcg gtgctcggcc gcgccgtact caggatcgcc gaccgcgacg gccgcgcccg
      121 ccacctcgtc ggtggcgtcg tggtagaagg gcacggccag cagatgcacg ggctctcccg
      181 tgcggtcgcg cgcctcggcc cgcgccggct cctcctcgtc catcgccgta cgggccaccg
      241 cgcgaagagc cccggccggt acggcgaact gttcggtgcg gccccggaac acggtcaccg
      301 gatcaccgcc gggccgtccc tccagcacca ggatctgcgg ggtgcccgag gtcgcatcgt
      361 cgtcgtcgag gccgtccagc cggatgcggt cgtcctcgta gtagacgagc gaggccgcga
      421 cggacgcccg gcggcgcatc tcgtcgtact ccgcgtcctg ccgcgcccgg tcgtcggtac
      481 ggatcaccag ccaggacagc gcggccaggc cgacgacggt gatcagggtg tacgcggcgg
      541 tcagccgcag ctgcagccgg cgcagccgcc tccgggccgg ggaacgacgc cgtacgggct
      601 tcgccatcag gacggccccg gctccgccga cagcaggaac ccctgaccgc gcacggtgtg
      661 cacgaggccg ggggagccga gcttgcgccg cagccccgcg acgacggcgt ccaccacatt
      721 ggacaccggg tcggccatct cgtcccagca gtgctccagc agatcggtac gggtgacgac
      781 ggcgtcccgg tggacgagga gctgctggag gaccgcgtac tccttcgggc tcagcgacag
      841 cagcacgccg ccgcgccgca cctcgtaccg cgccacgtcc atgaccacat ccgcacagct
      901 caggaaggac ggcagccggg ccgccgcccg ccggctcagg ccccggaccc gcagcaccag
      961 ctccggcatc gcgaacggtt tggccagata gtcgtccgcg ccgctctcca gcccccgcaa
     1021 ccgctcgggc agcgagtcca gggcggtgag acacagcacg ggcaccgacc agccggcccg
     1081 gcgcctgacc tccagcggga cgagggtgtc gccggagggc accatccggt ccagcaccac
     1141 acagtcgtac gcggtgacgt gcagcagcac atccgcctcc ggccagtccg ccgcgcagtc
     1201 cacggcgaag cccccgtccc gcagcccgga cgcgatgacc tcccgcaggt cgtcgtcgtc
     1261 ctccaccagc agcacacgca tggggaggac gcctccgatg tcgttccaag ggggagagac
     1321 ccgccatcct aaggcgtccc gcgcgcccgc cccgaggcgg ccgtcagacc ggtgaggcct
     1381 cgatgatccg gaagatgtcg ggcgcgggca acggcgtcac gctccggagg gcgaagccgc
     1441 ccgccgcgca cagggcggcg aagtcgtcct cggtccgctc ccggccgccg acattgacga
     1501 gcatgttgag atcgctgagg tagacgcctc ccgccgggtc cgccggcacc gtcgcgggca
     1561 gcaccggctc gacgatcagc agcgaaccgt ggtcggggat gacggcccgg atgcgccgca
     1621 ggatggccgc gcaccgctcg tcgtcccagt cgtggctgat gctcttcagc agatagaggt
     1681 cgccgccggc cggggccgag gcgaagaagt caccggtctc caggacgacg cggccggtca
     1741 catcctgctg ggccagccgt cgcggagcct gcgcgagtcc ctcggccgtg tcgaacagga
     1801 cgccccgcag ctccgggtgc gcgcgcagga ccgaggccag cagggtgccg tcgccgccgc
     1861 cgatgtccac gagcgtgcgg aagcgcccga agtcgtagtg gtgcggcacg ttcgaggcgg
     1921 tcagccgggt gccctggctc atcgcctcgt tgaacgcggc tgacagctcg ggctgctcct
     1981 tgagatggcc gaagaagtcc gtcccgaaca ccgtgtcgaa cgtggtctct cccgtacgga
     2041 cgctctcgtc gagcaggtcc cagccgcgga gcatgaccgg atcggtgaac atccgggcca
     2101 tggcgaccat cgtgccgggc gtgtcggtgc gcagggcgtc gcccgccgtc gtggtccgga
     2161 agacgcccgg ctccgtctcg gacagcaact ggagtccggc gagcgcgcgc agcagacgca
     2221 gtgtcgcctg cggatgggtg ccgagagaac cggcgagcgt gtcgccgctc agctcgccag
     2281 gaccgatccg gtcgaacacc cccaaccgca ccgccgtgcc gagtgcttgg gtggccatct
     2341 ggccgagcac gagccgggcg accgtacccc gcgcctgcca atcgaccgcc gtctcccggc
     2401 cgttgccggc ttcctggctc tgactgcccg tcatcgcacg cccctctctg gttggttagg
     2461 taaacctaac ttcgtccgga gggtgccgca atcacccata cgcgggggtg ggtggaggcg
     2521 aggtcgaacc ggtcagtccg gccggtcgcc gtcgagcgcc tcggccgcct cggccgccac
     2581 cgcgtcggcc acggccgcca gagccggcga gtccagcttc cactgctgcc agtacagcgg
     2641 gacgacgacg ctccggccgg gggcgtcgtc cggggcgagc cgcaccagcc ggccgtcgcc
     2701 cagcaacggc tccgcctgga cctcgggaac catgccccag cccatgcccg ccgccacggc
     2761 gttcacgaac ccctccgagg tgggcaccag atgacgccgg gcgctcgccc ggggcccgag
     2821 gccgagccgg cgcacgaacg cgtcctggag atcgtcgcgc cggtcgaagc cgatcaccgg
     2881 ggcatcgccg atcagctcgt gcagcgccgt gccggcccgc cgctccagcc accggtcggc
     2941 gaaagcggga ctggccaccg gcagataccg catccggccc agtctgcgca ccgaacagcc
     3001 cgtcaccgct tccggggacg aggtcaccgc cgccatcacc agcccctccc gcagcagggc
     3061 ggccgtatgg tcctggtcct cgcggcgcag ctcgtagcag aggtccaact cctccggcac
     3121 ccgccgcagg gcgggcagga accaggtcgc cagggaatcg gagttcaccg cgatcgacag
     3181 gggtgtcggc ccgtccggac ccgccatccc gagcgccgtc cgcgcatcgc gctccagccg
     3241 ggacagctgg cgcgcgaagc gcaccaccac ctcgccggac tcggtgggcc gcaccggctt
     3301 cgtgcgcatc agcagcaccc ggcccgtgcg ctgctccagt gccttcacgc gctggctgac
     3361 cgccgacggc gtcagctgca ggacgcccgc cgccgcgtcg aaggtgcctt cgtcgaccac
     3421 ggccagcagc gtccgtacct ggtcgagagg aagctcgaac atcatgaacg ctaatgatac
     3481 gtaagaattc ttagctggac atcacattgt cccgctttgt agcgtcggcc gcatgatgaa
     3541 cgccctgacc gccgcggccg ccggattcgg caccggcctc tccctcatcg tcgccatcgg
     3601 cgcccagaac gccctggtgc tgcgccaggg cgtccgccgt gacggtgtgc tcgcggtggt
     3661 cgggatctgc gccctgtccg acgcggtgct catcaccctg ggggtcgccg gggtcggcgc
     3721 ggtcgtcgtg gcctggccgt cggcgctgac ggtggtgggg ctgctcggcg gcctgttcct
     3781 gatcggctac ggtctgctcg ccgcacgccg cgtcctgcgc ccctcggccc tgtacgccgc
     3841 cgacgcgccg gccacgtcgc gccggggcgt cgtgctgacc tgcctggcga tgacctggct
     3901 caatccgcac gtgtacctcg acaccgtgtt cctgctcggt tccgtcgccg ccgaccgggg
     3961 tccgctgcgc tggaccttcg gcctcggcgc ggtcctcgcc agcctgtgct ggttcgtcgc
     4021 gctcggatac ggcgcccggc tgctgagcgg ggtcctggcg cggcctgccg cctggcgggt
     4081 gctggacgcg ctggtcgccg tgatgatgat cggcctcggc gcgaaactgg cgctggacgc
     4141 ctgaacgccc ggcacccgtc ccgcgtacga acctgaaagc cgggcggccg ttcccccggc
     4201 ccggttcagc cgaccggatc cagcaggccg aggcgctcgg cgcgctccgg tgtcaggtcg
     4261 agggcggtga ggatggaggg cgtggcgata ctggggagta tgtgctgctg cagcgtgatg
     4321 tggcggcgct gcaggtcctg gtagttggtg agagtctggg agaccgcctg ggttcccgcg
     4381 aaggcggcca cgtacaggtc cgccgtctcg gtggggttga cgtgggggag cagctcgccg
     4441 ttgtgtttgg cacgggtgag gaggtgatgg gtggcttcct gccagcggag aaaggggccg
     4501 ctgcggtcga ggccctgtgc ctgctggtcc atggagaggc ggacgcccgc ccgggcgagg
     4561 aggctggtct gcagacgatg gctgaacagc atgcccaggt cgatgagttc ctggagtttg
     4621 agagcctggt ccggaacggt ctgaggtggc tcctgcgcat cgagaacacc gagcgcgaga
     4681 tcttccttgg acgggaaatg gaagtagagg gcgcctttgg tgacgccgga gcttttgagt
     4741 atctcggtga tcgtcgcggc ttgatagccg tatttctcga aggcctgcgc cgctgccatc
     4801 aggattgtct ggcgggtgcg gatcgcccgg tcctgcttgg ccatcctgtc tcctttgctg
     4861 ggcgagggcc cgggaaacgg ctgggacggt gtggggggac ggccctgccg gggatagaaa
     4921 aaaaaccgct tagtctgtat cttatcagcg cacgtgcatc gcaggaacgg ctcgccatca
     4981 catgacccct ggggggagtt atgaccgacg cagcagtctt gatcgatccg gtgccggcag
     5041 tggaatcgga ggccgaggtg gtgcatcccg tggggatcga catggtgcac cggtccaggc
     5101 cggaggacgc gtttccgcgt cactgggtgc gcctggcacg cgatcggttc tcggtcgcgg
     5161 cggtcctgcc gcacgatcat ccgttcttcg ccccggtgag cggcgaccgg cacgatccgc
     5221 tgctggtggc ggaagcgatg cgacaggcgg ccatgctctc cttccacgcc gggtacggcg
     5281 tcccggtggg ctaccacttc ctgatggcgg acctcgacta cgcgtgcgac ctggagcacc
     5341 tgggcgtcgg cggcgcgccc gccgagatcg acgtggaggt gttctgctcc gacctgaagt
     5401 ggcggggcgg actgcccgtt caggggcgcg tgggctgggc ggtccaccgc gacggggaac
     5461 tggccgcgac ggggacgggc gcgacccgct tcacgaaccc caaggtgtac cggcggatgc
     5521 gcggtgacac acccgccgag gggatatcga taccgcgggc tacgccggtc tccaccgccg
     5581 gggcgggccg caccagggcc gaggacgtgg tgctctcgga ggccggccgg gacggtgtgt
     5641 gggaactgcg cgtggacacc cggcacccga ccctgttcca gcggcccaac gaccatgtcc
     5701 cgggcatgct gctgctggag gcggcccgtc aggcggcctg cctggcggtg ggacccggcg
     5761 gaatcgttcc ggcggaggcg cgcagccggt tccaccggta cgccgagttc gacagcccgt
     5821 gctcgatcgg ggcggtggtc cggccggggg cggccgagga tacggtgacg gtccaggtga
     5881 caggccatca ggacggcgaa ctggtgttct ccaccgttct gtccggcccg cgggtcaacg
     5941 gctgagaggt ttccgagtga ttctcgtgac gggtgcgacc ggagcggtgg gccgtgaggt
     6001 cgccggacag ctggaggcgg caggcccggt gcggattctc gcacgcagtc ccgagcggct
     6061 gacggtacgg gggagcgggg tcgaggtcgt cgagggcgag tacggggacc gctccgccct
     6121 cgaccgggcc atgcggggcg tcagctcggt gttcctggtg acgaagagcc ccacggagcc
     6181 ccacgacgag tgggtcgccg cggcggcggc agcggcgggc gtccgccacc tggtcaagct
     6241 gtccatgatg gcggtggagg agccgggggc cgacgacttc atcacccggc gtcagcggga
     6301 gaacgaacag gccgtgcggg agtcgggggt cgcctggacg ttcgtccgcc ccaggacgtt
     6361 catgtccaac acgctgtcct gggcgcccgg catccggtcg gccggagtgg tgcgcgcgct
     6421 gtacggcgac gcgccggtcg cgtgcgtcga cccgcgcgac gtcgcggcgg tggccgtcac
     6481 cgcactcacc ggtacgggtc acgagggcag ggcgtacgcc gtgtcgggcc cggaggcgat
     6541 caccgcgcgg gagcagacgg ccgagctctc ccgggtgctg gagcggccgt tgcgcttcga
     6601 ggaactgggc gtggacgccg cgcgcacggc cctgctggcg aggtacccgc ggcccgtcgc
     6661 ggaggccttc ctggagagcg cggagcggca gcgggcgggc gcgaaggcgg cggtggtgcc
     6721 caccgtcgag gaattgacag ggcgaccggc ccggccgttc cggatctggt cggccgatca
     6781 cgcggacgcg ttctcggcgt aatacggcgt gacagatctc caataagtgc gatgatccgc
     6841 cctttttctt ttccctttcg gtccggtttg acggccctga agggaaggag tggccggaaa
     6901 gggatcccga agcctccacc gtgttcttcc gttcgctgga aatgaaaccg gtaagttggt
     6961 ttgataactc catgaagccg tcccactgcc ccggcgggga gccctccacc ggggcagccg
     7021 ccttttccgc ccccctgccc ggccgccgat tcttcgaacg gaggccccgg tgaccaaaca
     7081 ggagcgggcc acccggaccc gccggtcgct gatccgatcc gccgccgtcg tcttcgagca
     7141 gcacggctac gcgcaggcgc ggctgacgct gatcagttcc ggcgcgggcg tcagcacggg
     7201 cgcgctgcac ttccacttcg agaacaaggc cgcggtagcg gatgccgtgg tggtggaggc
     7261 gtcccgggag ctgcgcgaga tgtccggcgc gatccggcgc aggacggaca ccgccctgca
     7321 ggcgctcgtc gacagctcgc acgctctcgc ggaacggctg cgcgaggacc cggtgagccg
     7381 ggccggattc cggctgagct gcgacgccgc gggaaccgcc gccccggacc tgagagtgca
     7441 gtggcaccac cgtgtgcggg aactgctgga cgacgcggcc gccgccggaa cactcgccga
     7501 cgacgtctcc cgcgaggacg cggccgccgc cacggtcgcc gcgacggccg gattcgaggt
     7561 gctcggccgg cacgatcccg ggtggctgtc cccgtacacg ctcaccggct tctggcagtt
     7621 gctgctgccc cgcctcgccg ccgcggagac cctgccgctc ctggacccgg ccggcacggg
     7681 tgcgacgtcg tcgccccgga ttcccgcgcc agcggcgggc gcgaccgatg aggcggaggc
     7741 ggcggcagga gtggcgtccg gggaggtggc gtcggcggcg gtggccgaag ccgactgaac
     7801 tgaccggtcg tccgcccggc gagcgccgct gctccgccac cctgagcctt ctgtaccttc
     7861 cgcagcatgg ctgacgagat gttcgtggat ccccgactgg ccgctctcta cgacgcgctc
     7921 gaccccgacc gaggcgacct ggacccgtat gtccgcatcg ccgaggagtt cggtgcacgg
     7981 cgggtgctgg acatcggctg cggcaccggt gtgttcgccc tgctgctggc ggaccgcggg
     8041 ttcgaggtcg tcggcgtcga ccccgcccgg gcctcggtcg acgtggcgcg cgccaagccg
     8101 ggcggtgacc gggtccgctg gatcagcggt gacgcttcgg ctcttccgcc gctgcgggtc
     8161 gacctggtca ccatgacggc caacgtggcg caggagatca ccggcccgcg cgactggcgg
     8221 gcgacgctgc gcggcgccta cgaggcgctg agaccgggcg ggcatctggt cctcgagacg
     8281 cggaacccgg cccggcgcgc ctgggaggag tggaaccggg agcgctcgca ccgtgtcacc
     8341 gagatcgccg gcctcggccc cgtcgagagg tgggtcgaag tgaccgaggt cgccgggccg
     8401 ctcgtgtcgt tccgctggac gtacgtcttc gcggccgacg gccaggtcct cacctccgac
     8461 tccacgctgc ggttccgcga gcgtcaggag gccgaggcgg acctgaccgc ccagggcttc
     8521 gtcgtgcgcg aggtgcggga cgcacccgac cggccgggcc gggagttcgt cctcatcgct
     8581 cgtcgtccgg accccgcccc gccccgctga tcacatccgc ccggcccgcg tcggcgcccc
     8641 agctcgccag caggcgcagg gcctgggccg actccgaccc cggctccgcg tggtaggtca
     8701 ccagctgctg gtcctcgtcg tccgggaggt tcagcgtctc gtacgacagg gtcagatcgc
     8761 cgacgagcgg atgccggatc agcttgatgc cgtggccctt ctccttgacg ttgtgcgtcg
     8821 cccacagccg ccggaactcc tcgctcttca gcgagagttc gcccaccagt gccgacagct
     8881 gcggatcgtc ggggtggcag cccgcgtaca gccggagata gctgaccatg tccgaggcct
     8941 tggagtccca gttcaggaac aggtcccggt aggcggggga gaggaacacc aggcgcgccc
     9001 agttccgctc ggccggcggc agtgcggccc agtcgccgaa caccgccgcc gccatcggat
     9061 tccaggcgag gatgtcggac cgcgccccgg ccacgtacgc gggaatgccc tccatgttgt
     9121 ccaggagata cagcagcccg gtccgcaccc gctgcacctt cgccgggcgg cgcttcttct
     9181 tgtgccgggc cggcctcgcc agatggttga gatgcgcgtg ctcggcgtcg gtgaggcgca
     9241 gcgcgcgggc gatcgcgtcg agcacctcgg ccgacacatt gcgcccgttg ccctgctcca
     9301 gacgcgtgta gtacgccacg gacaccccgg ccagctgcgc cagctcctcc cggcgcagcc
     9361 cgggcacccg acgggcccgg ccgaactccg gcagtcccac gtcctgcggc tgaagcttgg
     9421 ccctgcgggt gcgcaggaac tcgctgagct cggcacgccg gtccagcggc tccggcgcgg
     9481 cggcctccgg ctgatcgtcc atgggttcca ttattcctgc gatcagtggt cgtaggcggc
     9541 cgagcctgtc cccgtcagta gtaggaccac cggacgtagg caaagcggtg ggctgggtaa
     9601 acgtcggacc cggagtgagg ctgggacacg ttcctaccgc atcacccaga ggagaacctc
     9661 cggcatgacc accgttgccg cctacgccgc tccccgtgcc aaggccccgc tggagcgcac
     9721 cacgatcgag cgtcgtccgg tgggcgagtt cgacatcctg atcgacatca agttcgccgg
     9781 catctgccac tccgacatcc accaggcccg cgacggctgg ggcgagggca ttttcccgat
     9841 ggtgcccggc cacgagatcg ccggcatcgt cgccgagacc ggctccgggg tcacccggtt
     9901 caaggtcggc gaccgcgtcg gcgtcggctg catggtcgac tcctgcggaa cgtgcgacgc
     9961 ctgcctgatg ggccgcgagc agcactgcgc cgagggcaac acccagacgt acaacgccct
    10021 cgaccgcagc ggcgagccca cctacggcgg ctactcgacg cacatcgtcg tgaccgagaa
    10081 gttcgccgtc accatccccg agggcatcgc cctcgacgag gccgcgccgc tcctgtgcgc
    10141 cggcatcacc acgtactcgc cgctgaagcg ctggggcgcg ggccccggca agaaggtcgc
    10201 cgtcgtcggc ctcggcggtc tcggccacat ggccgtcaag atcgcgcacg cgctcggcgc
    10261 cgaggtcacg gtcctctccc agtccctgcg gaagaaggac gacgggctca agctgggcgc
    10321 cgaccactac tacgcgacca gcgaccccaa gaccttcgag gagctggccg gaaccttcga
    10381 cgtcatcctc tccacggtct cggccccgct ggacttcggc gcctacctcg gcctcctgcg
    10441 cacggacggc accctcgtca acgtgggcgc tcccgaggag ccggtctcgc tgaacctctt
    10501 ctcgctgatc atgggcaacc ggtcgatcgc gggttcggcg atcggcggta tcgcggagac
    10561 ccaggagatg ctggacttct gcgccgtgca cgggctcggc gcagagatcg aggtgatcgg
    10621 cgcgagccag gtcaacgagg cgtacgagcg ggttctcgcg agcgacgtcc gctaccgctt
    10681 cgtgatcgac acggcgacca tctgagccac cacggcccgc cctcaccatg cgggcgaccg
    10741 cccggccgtg tgatctccgc aggcgtccgc cccgagcacc aggtgctcgg ggcggacgcc
    10801 tttccgcccc agcgcaaagg gcccgaccgc ccaccgggcc gcacggcccg atctcgctcc
    10861 gcgcggtcac ttcgccatcg cgcgcgggcg gcccggaccc ggccgacaat ggccagaggc
    10921 agtcctacgg gcgtcaccgg gggcc
//
