LOCUS       NZ_KL503830            11311 bp    DNA     linear   CON 02-MAY-2024
DEFINITION  Streptomyces griseorubens strain JSD-1 scaffold1, whole genome
            shotgun sequence.
ACCESSION   NZ_KL503830
VERSION     NZ_KL503830.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces griseorubens
  ORGANISM  Streptomyces griseorubens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces althioticus group.
REFERENCE   1  (bases 1 to 7563100)
  AUTHORS   Feng,H., Zhi,Y., Sun,Y., Wei,X., Luo,Y. and Zhou,P.
  TITLE     Draft Genome Sequence of a Novel Streptomyces griseorubens Strain,
            JSD-1, Active in Carbon and Nitrogen Recycling
  JOURNAL   Genome Announc 2 (4), e00650-14 (2014)
   PUBMED   24994798
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563100)
  AUTHORS   Feng,H., Sun,Y., Zhi,Y., Mao,L., Luo,Y., Wei,X. and Zhou,P.
  CONSRTM   Shanghai Jiao Tong University
  TITLE     Draft genome sequence of the novel Streptomyces griseorubens JSD-1
            playing a role in carbon and nitrogen cycle
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7563100)
  AUTHORS   Feng,H., Sun,Y., Zhi,Y., Mao,L., Luo,Y., Wei,X. and Zhou,P.
  CONSRTM   Shanghai Jiao Tong University
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2014) School of Agriculture and Biology, Shanghai
            Jiao Tong University, Shanghai Jiao Tong University, Shanghai,
            China, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2013-12-24
            Genome Coverage                   :: 246x
            Sequencing Technology             :: Illumina Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000709915.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 03:19:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,036
            CDSs (total)                      :: 6,948
            Genes (coding)                    :: 6,620
            CDSs (with protein)               :: 6,620
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 329
            CDSs (without protein)            :: 328
            Pseudo Genes (ambiguous residues) :: 0 of 329
            Pseudo Genes (frameshifted)       :: 72 of 329
            Pseudo Genes (incomplete)         :: 272 of 329
            Pseudo Genes (internal stop)      :: 8 of 329
            Pseudo Genes (multiple problems)  :: 23 of 329
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:40:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5991671
            Orig. end                         :: 6002982
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KL503830.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11311
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="14"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11311
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11311
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6311](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6311)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     assembly_gap    511..535
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <536..1159
                     /locus_tag="DJ64_RS38365"
                     /old_locus_tag="DJ64_15480"
                     /pseudo=""
     CDS             <536..1159
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445289.1"
                     /locus_tag="DJ64_RS38365"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DJ64_15480"
                     /product="protoporphyrinogen oxidase"
                     /pseudo=""
                     /transl_table=11
                     /translation="AAPAPAAADLLRAEAPGAAAELSAVEYASMALITLAYRRDEAHLP
                     EGSGFLVPPVEGRSIKASTFASRKWGWIAEENPDLAVVRTSVGRHGETELLHRDDDELV
                     ELSRHDLKAATGLDASPVAARVTRWDDGLPQYPVGHHARVARVRAHLAGLSGLAVCGAA
                     YDGVGVPACIASAYAAADQIQGDLRAVQQLTAHPVQSLHGGAGE"
     gene            1165..1896
                     /gene="hemQ"
                     /locus_tag="DJ64_RS26795"
                     /old_locus_tag="DJ64_15485"
     CDS             1165..1896
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="DJ64_RS26795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DJ64_15485"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_033274023.1"
                     /transl_table=11
                     /translation="MSDDATTPAPDRIPNKGKLAKDLNEVIRYTLWSVFKLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDVSGLRADADLMIWWHAETADQLQEAYNLFRRTR
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARMHVR
                     EEVPFFTGRRKDVADLVAGLA"
     gene            complement(1901..3457)
                     /locus_tag="DJ64_RS26800"
                     /old_locus_tag="DJ64_15490"
     CDS             complement(1901..3457)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (103-476). E-value:
                     8.4e-20. Score: 63.3. Matches aSDomain:
                     nrpspksdomains_DJ64_RS26800_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523451.1"
                     /locus_tag="DJ64_RS26800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DJ64_15490"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_037642267.1"
                     /sec_met_domain="PF00561 (E-value: 1.9e-23, bitscore: 82.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAALVAAGSLLLTTPAPASPSTSASEARGTAVAAARAASEGID
                     FGPCTEEQELPPTVECGTLSVPLDYARPDGERIRLTVSRSRATGKDPATGRAVPRQGSL
                     VNNPGGPGVSGMYFPMLGVVPGWKRLAAAYDLVGYAPRGVGRSAPLSCTDPATFHQGPA
                     PAPTHPTAAVKRERAARAAAYARGCAERAGDALQHYTSPNNARDLDVLRAALGETRLTF
                     LGSSYGTYVGALYATLFPSHVRRMVFDSAVNPAPDRIWYRNGLARSAAFEDRWADFRAW
                     AARHHAVYGLGDTARKVGDSYDVVRERLAREPAGGKVGPAQLHASMLKAAYYDDHWPQR
                     AHALSAYLKGDPAPLVEQAEEQPETAAEAENTRAVYLAVQCNDAPWPTDFAVWDRDNTR
                     LARRAPFGTWDNVWTNLPCASWPAPRQRPLDVRAGAGELPPTLILAAERDAATPYDGAL
                     ELHRRLAGAALVTERDAGSHGLAGGANACVNAHLTAYLLEGRVPGRRASCAPHPAPAPR
                     A"
     aSDomain        complement(2030..3148)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DJ64_RS26800_Abhydrolase_1.1"
                     /evalue="8.40E-20"
                     /label="DJ64_RS26800_Abhydrolase_1.1"
                     /locus_tag="DJ64_RS26800"
                     /protein_end="476"
                     /protein_start="103"
                     /score="63.3"
                     /tool="antismash"
                     /translation="LVNNPGGPGVSGMYFPMLGVVPGWKRLAAAYDLVGYAPRGVGRSA
                     PLSCTDPATFHQGPAPAPTHPTAAVKRERAARAAAYARGCAERAGDALQHYTSPNNARD
                     LDVLRAALGETRLTFLGSSYGTYVGALYATLFPSHVRRMVFDSAVNPAPDRIWYRNGLA
                     RSAAFEDRWADFRAWAARHHAVYGLGDTARKVGDSYDVVRERLAREPAGGKVGPAQLHA
                     SMLKAAYYDDHWPQRAHALSAYLKGDPAPLVEQAEEQPETAAEAENTRAVYLAVQCNDA
                     PWPTDFAVWDRDNTRLARRAPFGTWDNVWTNLPCASWPAPRQRPLDVRAGAGELPPTLI
                     LAAERDAATPYDGALELHRRLAGAALVTERDAG"
     gene            complement(3557..4339)
                     /locus_tag="DJ64_RS26805"
                     /old_locus_tag="DJ64_15495"
     CDS             complement(3557..4339)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523450.1"
                     /locus_tag="DJ64_RS26805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DJ64_15495"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_037642270.1"
                     /transl_table=11
                     /translation="MFWVPLLLLAWAIAGVVGVRLCRAAVREAAAAGDGAGERRGLTLY
                     EAAFLSGGPARVADLTLVAMARQRRLLLAHTGWATVVDPRGRDDMERTVIGAIGPEGQS
                     RIAPVRKAAATAEAVRTLAERLVRAGLAVPEGDGRTVARALRRTRLAVPAITGLAAVAL
                     LMPVPSQMPRLLVALWFALPLAFTLGCLAIARFELSPASPWASPAGRRLLASARRAAAG
                     DTDDDRAYLTSVAVSGVHAVGEPQVRAAFAHREPRVEW"
     misc_feature    4333..4346
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.76"
                     /tool="antismash"
     misc_feature    complement(4446..4461)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.49"
                     /tool="antismash"
     gene            4720..4950
                     /locus_tag="DJ64_RS26810"
     CDS             4720..4950
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DJ64_RS26810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_051724431.1"
                     /transl_table=11
                     /translation="MEDPYATVEIRNPNAHEAVLEVKIHFVDAYGSPLLDTSNQVVVAA
                     KDELAYRVPAKFQYTDRIAHCEVEPVVRAVR"
     gene            complement(5001..6311)
                     /locus_tag="DJ64_RS26815"
                     /old_locus_tag="DJ64_15505"
     CDS             complement(5001..6311)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130505.1"
                     /locus_tag="DJ64_RS26815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DJ64_15505"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_033274020.1"
                     /sec_met_domain="DUF692 (E-value: 2.4e-97, bitscore: 324.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVRLGTGIGWRPEIADAVEAMPGIDWVEAVAENVCPGHLPASL
                     LRLRERGTTVVPHGVSLGLGGAERPDEGRLTALAERASALGSPLVTEHIAFVRAGGPLT
                     ASPGIEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALFSWPGEEMTEGQF
                     LYELADRTGVQLLIDVANLHTNHVNRGEDPAKALAELPLEAIAYVHVAGGFERDGVWHD
                     SHAHPVPRPVLDILTDLASRVSPPGVLLERDENFPPPGELERELDAIRGAVETGDRARV
                     PSAPEPVREPAAAEPVAGATRERLALAQTAVLSSLVAGTPVPEGFDRTRMRVQARALAA
                     KRADVVAKVAPELPEILGDGYRPAFLAYAQTRPMTGGYRHDAMAFVAHLLHPGRLDDPR
                     TRRALREWHRDRSGPHPRSHRPTTRLARATRRALLRR"
     gene            complement(6554..8617)
                     /locus_tag="DJ64_RS26820"
                     /old_locus_tag="DJ64_15510"
     CDS             complement(6554..8617)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523447.1"
                     /locus_tag="DJ64_RS26820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DJ64_15510"
                     /product="neutral/alkaline ceramidase"
                     /protein_id="WP_037642272.1"
                     /transl_table=11
                     /translation="MTAERPPLRSRRTVLRAGAVTGLGLAGAHLVSPGTAAAAAGSGGG
                     YLVGRGISDATGEVAEVGMMGYGRFDQQAAGLHTRLRARAFVVAEQASGRRVLLIVADS
                     PMIFESVHRAVLRRLKQAYGDLYTARNVLITATHTHAGPGGYSHHLLYNTTTFGFHKAT
                     FEAVADGLFEAARNAHDDLAPSELLLSHGTLTGASVNRSKTAFDRNPTADRDHFPDGVD
                     TLTTLLRVEREGRTVGAVNWFPVHGTSMSGDNRLISADNKGYAAYHWEREVHGVDYLSD
                     GTPEFVSAFAQTNSGDMSPNLDLVPPTTPADFDATRTSGLRQYEAAAAQAEAAGTRLSG
                     PVDSRLVYVDLSDVTVRPEFTGDGKTHRTSKPCVGAAMAAGSLEDGPAFPGFDEGENPF
                     WDAISNSIVYTVSPELKQAQAPKDVFVPIGEMNRIYPWVQERVPVMLVRIGGLYLIGIP
                     GEVTVCAGLRLRRTVAGIVGADLDDVLVAGYSNSYFHYVTTPEEYDAQQYEGGSTLFGR
                     WQLPALQQTAADLAVSLRDGTDLPLGAEPPDLTGKALSLQPGVVLDAPPLFRKFGDVLV
                     PPRENYRTGERVEVVFAGAHPSNDLHRGDTYLRIERRDSGGAWRTVADDGDWATRFHWA
                     RDGIAASKVTITWDIPQGTPSGTYRVVYRGDAKSLGGKISSIEGVSTAFQVR"
     misc_feature    complement(8628..8647)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.35"
                     /tool="antismash"
     misc_feature    complement(8630..8644)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.9"
                     /tool="antismash"
     gene            complement(8803..10398)
                     /locus_tag="DJ64_RS26825"
                     /old_locus_tag="DJ64_15515"
     CDS             complement(8803..10398)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (116-294). E-value:
                     2.8e-15. Score: 48.5. Matches aSDomain:
                     nrpspksdomains_DJ64_RS26825_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130508.1"
                     /locus_tag="DJ64_RS26825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DJ64_15515"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_037642273.1"
                     /sec_met_domain="PF00561 (E-value: 4.8e-25, bitscore: 87.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTRRTTAAALLSGAVLLSASLAACGPAGEGGAGGGGGEEDDRGR
                     IWAQSLNWKDCPAPSPLQGGGEAPGPLPDGTRWQCATMRAPLDWDRPGGETVDVALIRA
                     ASRADAADRIGSLLFNFGGPGESGVATLPALAPDYEQLRSRYDLVSFDPRGVGASAGVT
                     CLDAEAAQAMAQQDDTPDDGDDEVTELLQARQRYADACERESGRVLPHVGTEDAARDMD
                     LLRHLLGDDELHYFGISYGTELGGVYAHLFPRHVGRAVLDGVVDPTLDQMEGALAQAGG
                     FQLAFDHFAQWCVDTGCALGDTPEEITRLVMELEETLDDEPLSVGGDRVFTGSQLIDAV
                     VQALYLETAWPALEVGLQLLAADEGETLLGLADALSGRHAGGWSDQQDAQTAITCADRS
                     DRYTVEDVLEQAAAFEEASPLFGDTMVWGALECTDWPVRGKAPHPDVSAPGAPPILLVG
                     NTGDPATPYEGAARMAERLGKGVGVELTYEGEGHGAYDSGNACVRKAVNAYLLDGTVPA
                     AGTVCAREPLLGKE"
     aSDomain        complement(9517..10050)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DJ64_RS26825_Abhydrolase_1.1"
                     /evalue="2.80E-15"
                     /label="DJ64_RS26825_Abhydrolase_1.1"
                     /locus_tag="DJ64_RS26825"
                     /protein_end="294"
                     /protein_start="116"
                     /score="48.5"
                     /tool="antismash"
                     /translation="LLFNFGGPGESGVATLPALAPDYEQLRSRYDLVSFDPRGVGASAG
                     VTCLDAEAAQAMAQQDDTPDDGDDEVTELLQARQRYADACERESGRVLPHVGTEDAARD
                     MDLLRHLLGDDELHYFGISYGTELGGVYAHLFPRHVGRAVLDGVVDPTLDQMEGALAQA
                     GGFQLAFDHFAQWCV"
     gene            complement(10466..11185)
                     /locus_tag="DJ64_RS26830"
                     /old_locus_tag="DJ64_15520"
     CDS             complement(10466..11185)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130509.1"
                     /locus_tag="DJ64_RS26830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DJ64_15520"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_033274017.1"
                     /transl_table=11
                     /translation="MNDDRTTPAPDSPFRPDPGDRDAAPQFVLPLVVRLERGSPPARTD
                     ALETAARAVLVMLTDERSVGDGEWARAIRDWQDARIRKVVRRARGAEWRRAEALPGITV
                     TGKSAEVRVFPPVPLDGWPKDLARLQVSGTDLDDPDPVPPADPGVPTLWMNPELEMSAG
                     KAMAQAGHGAQLAWWELPHAARAAWHAAGFPLAVRTADPADWPALTGAGLPTVRDAGFT
                     EIAPGSCTVVADHPALR"
ORIGIN
        1 accgccgccg ccctcgccgg cgtgctctcc gacgagggcc tcgcccggat cgagcgggac
       61 gccgaactgc cgcccaccga gatcggcgac gacgtcgcca tcggcgagta cgtcgccgag
      121 aggttcggcc gtgaggtcgt cgaccggctc gtggagcccc tgctcggcgg ggtgtacgcg
      181 ggggacgcgt accgcatctc gatgcgctcg gccgtcccgg gcctcttcga cgccgcccgc
      241 gcgcacgcca ccctgaccga ggcggtccgc gccgtgcagg cgaagaccgc cgccgcgcag
      301 cggacaggac ccgtcttcac gggcgtcgcg ggcggtgtgg gcaccctgcc gctcgcggtc
      361 gccgacttcc tgcgcgcccg gggcgtccgc atcctcaccg gcaccccggt caccgagctg
      421 cgccgcgaac ccgacgacgg ctggcggctc gtcgccggcg gcgaggtgct gcacgccgac
      481 gcggtcgtcg tcgccgcgcc cgccccggcc nnnnnnnnnn nnnnnnnnnn nnnnngccgc
      541 gcccgccccg gccgccgcgg acctgctgcg cgccgaggcg ccgggcgccg ccgccgagct
      601 gtccgccgtc gagtacgcct cgatggcgct gatcaccctc gcctaccggc gcgacgaggc
      661 acacctgccc gagggcagcg ggttcctggt gccgcccgtc gagggccgct cgatcaaggc
      721 gtccacgttc gcctcccgca aatggggctg gatcgccgag gagaacccgg acctggccgt
      781 cgtccgcacc tccgtgggcc gccacggcga gacggagctc ctgcaccgcg acgacgacga
      841 gctggtggag ctgtcccggc acgacctgaa ggcggccacc ggcctggacg cctccccggt
      901 cgccgcccgc gtcacccgct gggacgacgg cctgccgcag tacccggtcg gccaccacgc
      961 gcgcgtcgcc cgcgtccgcg cccacctcgc cggcctgtcc gggctggccg tctgcggcgc
     1021 cgcgtacgac ggcgtcggcg tccccgcgtg catcgcgagc gcctacgccg ccgccgacca
     1081 gatccagggt gacctgcgcg ctgtgcagca gctcaccgcg cacccggtgc aaagccttca
     1141 cggaggagca ggagaataag ggacatgagt gacgacgcca ccacccccgc gcccgaccgg
     1201 atccccaaca agggcaagct ggccaaggac ctcaacgagg tcatccgcta caccctgtgg
     1261 tccgtgttca agctgaagga cgtcctgccg gaggaccgcg cgggctacgc cgacgaggtc
     1321 caggagctgt tcgaccagct cgccgccaag gacgtcacca tccgcggcac ctacgacgtg
     1381 tcgggcctgc gcgcggacgc cgacctcatg atctggtggc acgcggagac cgccgaccag
     1441 ctccaggagg cctacaacct cttccgccgc accaggctgg gccgcgcgct ggagccggtc
     1501 tggtcgaaca tggcgctgca ccgccctgcc gagttcaacc gctcgcacat cccggcgttc
     1561 ctcgccgacg agacgccccg cgactacgtg agcgtctacc cgttcgtgcg ctcctacgac
     1621 tggtacctgc tgccggacga ggaccgccgc cgcatgctcg cggaccacgg caagatggcc
     1681 cgcggctacc cggacgtgcg cgccaacacg gtcgcttcgt tctccctcgg cgactacgag
     1741 tggatcctcg ccttcgaggc cgacgagctg taccgcatcg tcgacctgat gcgtcacctg
     1801 cgcgcctccg aggcccgtat gcacgtccgt gaggaggtcc cgttcttcac gggccgccgc
     1861 aaggacgtcg cggacctggt cgccggtctg gcctgacctc tcaggcccgg ggagcgggcg
     1921 ccgggtgcgg tgcgcaggac gcgcgccggc ccggcacccg cccctccagc agatacgccg
     1981 tcaggtgcgc gttcacgcac gcgttggcgc cgcccgcgag gccgtggctg ccggcgtccc
     2041 gctccgtcac cagcgccgcc ccggcgagcc gccggtgcag ctccagcgcc ccgtcgtagg
     2101 gggtcgccgc gtcccgttcg gccgccagga tcagggtcgg cggcagctcg cccgcaccgg
     2161 cgcgcacgtc gagcggccgc tgccggggcg ccggccagga ggcgcagggc aggttcgtcc
     2221 acacgttgtc ccacgtcccg aacggcgccc ggcgcgccag ccgcgtgttg tcccggtccc
     2281 acaccgcgaa gtccgtcggc cagggggcgt cgttgcactg aacggccagg tacaccgcac
     2341 gcgtgttctc cgcctcggcc gccgtctcgg gctgctcctc cgcctgctcc accagcggag
     2401 cggggtcgcc cttcagatac gccgacaggg cgtgcgcgcg ctgcggccag tggtcgtcgt
     2461 agtacgccgc cttcagcatc gacgcgtgca gctgcgccgg accgaccttc ccgcccgccg
     2521 gctcccgcgc cagccgctcc cgcacgacgt cgtagctgtc gcccaccttc cgcgcggtgt
     2581 cgcccagccc gtacacggcg tggtggcggg cggcccacgc ccggaagtcc gcccagcggt
     2641 cctcgaacgc ggccgaccgg gcgaggccgt tgcggtacca gatccggtcc ggcgccgggt
     2701 tcaccgccga gtcgaacacc atccgccgca cgtgcgaggg gaacagcgtc gcgtacagcg
     2761 ccccgacgta cgtgccgtac gacgagccga ggaacgtcag ccgggtctcg cccagcgcgg
     2821 cgcgcagcac gtccaggtcg cgtgcgttgt tcggcgaggt gtagtgctgg agggcgtcgc
     2881 cggcccgctc ggcgcacccc cgcgcgtacg ccgccgcgcg ggccgcgcgc tcccgcttca
     2941 ccgctgccgt cgggtgcgtg ggcgcgggcg cggggccctg gtggaaggtg gccgggtcgg
     3001 tgcacgacag cggggccgaa cggcccaccc cacgcggcgc gtagccgacc aggtcgtacg
     3061 ccgcggccag ccgcttccag cccgggacca cgcccagcat cgggaagtac atgccgctga
     3121 cgcccggccc gcccgggttg ttcaccaggg agccctggcg gggcaccgcg cgcccggtcg
     3181 cggggtcctt cccggtggcc cgtgagcggc tcacggtgag ccggatgcgc tcgccgtccg
     3241 ggcgggcgta gtcgagcggg acgctcagcg tgccgcactc caccgtgggc ggcagctcct
     3301 gctcctcggt gcacgggccg aagtcgatcc cctccgaggc cgcccgcgcg gcggcgaccg
     3361 cggtgccgcg cgcctcggac gcggacgtgg agggcgacgc gggtgcgggg gtggtgagga
     3421 gcaaggaccc ggcggccacg agggcggcgg ttctcatcgg tgaggtgatc ccttcggtgc
     3481 gctgcggcga caaaagggat gttttcggtg tgcgtggtgc cgggcaagga ccgcccgccg
     3541 ggtgtcggcg acgggttcac cactccacgc gcggctcccg gtgcgcgaag gcggcgcgta
     3601 cctgcggctc gcccaccgcg tgcaccccgc tcaccgcgac ggaggtgagg tacgcgcggt
     3661 cgtcgtcggt gtcgccggcc gccgcccgtc gcgcggaggc gagcagacgc cggcccgccg
     3721 gggacgccca gggtgaggcg gggctcagct cgaaacgggc gatggcgaga cagcccagcg
     3781 tgaacgccag cggcagcgcg aaccacaggg cgaccagcag acgcggcatc tgcgacggca
     3841 ccggcatgag cagcgccacg gcggcgaggc cggtgatcgc cggcaccgcg agacgcgtcc
     3901 ggcgcaacgc ccgcgccacg gtccgcccgt cgccctccgg caccgcgagc ccggcccgca
     3961 ccagccgttc cgccagcgtc cgcaccgcct ccgcggtcgc cgcggccttc cgcaccggcg
     4021 ctatgcgcga ctggccctcc ggcccgatcg cccctatcac cgtgcgctcc atgtcgtccc
     4081 gcccgcgcgg gtcgacgacg gtggcccagc cggtgtgcgc gagcagcagc cgccgctgac
     4141 gggccatcgc gacgagcgtg aggtcggcga cccgcgcggg cccgccggac aggaacgccg
     4201 cctcgtacag cgtgagcccc cgccgctctc ccgcaccgtc accggcagcc gccgcctcac
     4261 gcaccgcggc ccggcacagc cgcaccccga cgaccccggc gatcgcccag gccagcagca
     4321 ggagaggtac ccagaacatg agttgtttgt aagggacacg gtcgcgaaaa caccatgcct
     4381 tgttcacgat ccggacacgg cgttgcggat atgtgacgtt ccgcggaacc gccctgtgcc
     4441 attctgctga cgatcatcag tgggaggggc ggcacgatgc gggtcaaagg aagtctgcgc
     4501 cgatggagct ggggggtgac ggtgacagcc gtggtgctgg cggccggttg ctcgcccggc
     4561 agtgactccg gtgcccccgt gaccgtcgcg ggtgggaacg gcgtcagtgc acagcccacg
     4621 cccgcagccg cagccacagc cacagccacg gtgtccgccg ccaccagccc agccccgccc
     4681 ctgaaggacg gaaccgccgt actcgtccgc tgcgcggaca tggaggaccc gtacgcgacc
     4741 gtggagatca ggaatcccaa cgcacatgag gccgtgctgg aggtgaagat ccacttcgtc
     4801 gacgcgtacg gctcaccgct gctggacacc agcaatcagg tggtcgtcgc cgcgaaggac
     4861 gagcttgcgt accgggtgcc cgccaagttc cagtacacgg acaggatcgc gcactgcgag
     4921 gtggaacccg tggtacgggc cgtccgttga agtgcggtgt gtccgagggc tgacggacga
     4981 acacccggtg cgggctggtg tcaacggcgc agcagggccc ggcgggtcgc tcgggccagg
     5041 cgggtcgtcg ggcggtggga gcgggggtgg gggcccgagc ggtcgcggtg ccactcgcgc
     5101 agggcgcggc gcgtgcgggg gtcgtcgagg cggccggggt gcaggaggtg tgccacgaag
     5161 gccatcgcgt cgtgccggta gccgcccgtc atcgggcggg tctgcgcgta cgcgaggaag
     5221 gcgggccggt agccgtcgcc gaggatctcc ggcagctccg gggccacctt cgcgacgacg
     5281 tccgcccgtt tggcggccag cgcccgcgcc tgcacccgca tccgggtccg gtcgaaccct
     5341 tcgggcaccg gcgtccccgc gaccagcgag gacagcaccg cggtctgggc gagcgccagc
     5401 cgctcccggg tggccccggc aacgggctcg gcggcggccg gctcccgtac gggctccggc
     5461 gccgaaggca cccgggcgcg gtcgcccgtc tccaccgccc cgcggatcgc gtccagttcg
     5521 cgctccagct cccccggcgg cgggaagttc tcgtcccgtt ccagcagcac gcccggcggc
     5581 gagacgcggg aggcgaggtc cgtgaggatg tcgaggaccg gccgcggcac cgggtgcgcg
     5641 tggctgtcgt gccacacgcc gtcccgttcg aacccgcccg cgacatggac gtacgcgatg
     5701 gcctccaggg gcagttcggc gagcgccttg gccgggtcct cgccgcggtt gacgtggttg
     5761 gtgtgcaggt tggcgacgtc gatgaggagt tggacgccgg tgcggtcggc cagctcgtac
     5821 aggaactgtc cctcggtcat ctcctcgccc ggccaggaga acagcgcggc gatgttctcc
     5881 acggcgagcg gcaccgggag ggcgtcctgg gcgatgcgga cgttctcgca cagcacgtcg
     5941 agggcgtcgc gggtgcgcgg caccggcagc aggtgcccgg cctcgatgcc gggggacgcg
     6001 gtcagcggac cgcccgcccg cacgaacgcg atgtgctcgg tgaccagggg cgaccccagc
     6061 gccgatgccc gctcggcgag cgcggtgagc cgcccctcgt cggggcgttc ggcgccgccc
     6121 agccccaggg agaccccgtg cggcacgacg gtcgtgccac gctcgcgcag ccgcagcagc
     6181 gacgcgggca gatggccggg gcagacgttc tccgccaccg cctcgaccca gtcgatgccc
     6241 ggcatcgcct ccaccgcgtc cgcgatctcc ggacgccacc cgatcccggt ccccagccgc
     6301 accgtcgtca tccgcgtccc ctccttcacc ccggacgctc ccgcccggcc cccgatccgt
     6361 ccgcacgggc gtgccggacg tgccggggat atgacccgcg gctcggcgca cgaaccgtcc
     6421 tgcggccgcg ttcagaggaa catttgaggt tggggaaggg ccgtcgggga acgccggccc
     6481 ccccacgcgt gggtggtggc gggcggcggg acgagcgtcg tgagccgtgt gatcatccgt
     6541 cggccggaca ggctcaccgc acctggaacg ccgtcgacac accctcgatc gacgagatct
     6601 tcccgcccag actcttcgcg tcgccgcggt agacgacgcg gtaggtgccc gagggcgtgc
     6661 cctgggggat gtcccacgtg atggtcacct tggacgcggc gatgccgtcg cgggcccagt
     6721 ggaagcgggt cgcccagtcg ccgtcgtcgg cgacggtgcg ccacgcgccg cccgagtccc
     6781 gccgttcgat ccgcaggtag gtgtcgccgc ggtgcaggtc gttgctcgga tgggcgccgg
     6841 cgaagacgac ctcgacgcgt tccccggtcc ggtagttctc ccggggcggc accagtacgt
     6901 cgccgaactt ccggaagagg ggcggcgcgt cgagcaccac gcccggctgg agcgacagcg
     6961 ccttgccggt caggtccggc ggctccgccc cgagcggcag gtccgtgccg tcgcgcaggg
     7021 agaccgcgag gtcggccgcg gtctgctgga gcgcggggag ctgccagcgc ccgaacaggg
     7081 tgctgccgcc ctcgtactgc tgggcgtcgt actcctccgg cgtggtcacg tagtggaagt
     7141 aggagttgct gtacccggcg accaggacgt cgtcgaggtc cgcgccgacg atcccggcga
     7201 ccgtgcggcg cagccgcagg cccgcgcaga cggtgacctc gccaggaatg ccgatgaggt
     7261 acaggccgcc gatgcggacc agcatcaccg gcacccgctc ctgcacccac gggtagattc
     7321 ggttcatctc cccgatcggc acgaagacgt ccttcggcgc ctgcgcctgc ttcagctcgg
     7381 gggacaccgt gtagacgatt gagttcgaga tcgcgtccca gaaggggttc tcgccctcgt
     7441 cgaagccggg gaaggcgggc ccgtcctcca ggctgcccgc cgccatcgcc gcgccgacgc
     7501 acggcttgga cgtgcggtgc gtcttcccgt cgccggtgaa ctccgggcgc accgtgacgt
     7561 cggagaggtc cacgtacacc agccgcgagt cgaccggccc ggacagccgc gtgcccgccg
     7621 cctccgcctg ggccgcggcc gcctcgtact gccgcagtcc gctggtgcgc gtggcgtcga
     7681 agtcggcggg cgtggtgggc ggcaccaggt ccaggttggg cgacatgtcg ccgctgttgg
     7741 tctgggcgaa cgccgacacg aactccggtg tgccgtcgga gagatagtcc acgccgtgga
     7801 cctcgcgctc ccagtggtag gccgcgtagc ccttgttgtc cgcgctgatc aggcggttgt
     7861 cgccggacat gctggtgccg tgtaccggga accagttgac cgccccgacc gtacggccct
     7921 cccgctccac ccgcagcagg gtggtcaggg tgtccacgcc gtccgggaag tggtcgcggt
     7981 cggccgtcgg gttgcggtcg aaggcggtct tcgagcggtt gacgctcgcg ccggtcaacg
     8041 tgccgtgact gagcagcagt tcggacggcg cgagatcgtc gtgagcgttg cgcgccgcct
     8101 cgaacaggcc gtccgccacc gcttcgaagg tcgccttgtg gaagccgaac gtcgtggtgt
     8161 tgtacaggag atggtgcgag tagccgccgg gacccgcgtg ggtgtgggtg gcggtgatca
     8221 ggacgttccg cgcggtgtac aggtcgccgt acgcctgctt cagccggcgc agcaccgccc
     8281 ggtgcacgct ctcgaagatc atgggggagt cggcgacgat cagcagcaca cggcgcccgc
     8341 tcgcctgctc ggccacgacg aacgcccgtg cccgcagccg ggtgtggagc cccgccgcct
     8401 gctggtcgaa acggccgtac cccatcatgc cgacctcggc gacctcaccc gtcgcgtcgg
     8461 agatgccccg gccgacgaga tacccgccgc cgcttcccgc ggcggcggca gcggtgcccg
     8521 gtgagaccag atgcgcgccc gcgaggccga gtccggtcac ggcgcccgcc ctcaggacgg
     8581 tccggcggct gcgcaaggga ggacgttccg cggtcatacg ctgctcctca gggtcggtcg
     8641 gccccccggc aacgaaacat gaacaacatt caagttccgc gcctcattgg tacaccggcc
     8701 gccatggaca cggcaagagt cgtgcgcgca tgacgcggcc ggcccggtgg gtccgtgtct
     8761 ccacggaccc accgggccgg ctcccgcctc aggacagcgg cgtcactcct tgcccaggag
     8821 cggctcccgc gcgcacacgg tccccgccgc cggcaccgtg ccgtccagca gatacgcgtt
     8881 cacggccttc cgcacacagg cgttgccgga gtcgtacgcg ccgtggccct caccctcgta
     8941 ggtgagttcc accccgacgc ccttgcccag ccgctccgcc atgcgcgccg cgccctcgta
     9001 cggcgtcgcg gggtcgccgg tgttgccgac gagcaggatc ggcggcgcgc ccggcgcgct
     9061 cacgtccgga tgcggggcct tcccccggac cggccagtcg gtgcactcca gcgcgcccca
     9121 caccatggtg tcgccgaaga gcggggacgc ctcctcgaac gcggcggcct gttccagcac
     9181 gtcctccacg gtgtagcggt cgctgcggtc ggcgcaggtg atggccgtct gggcgtcctg
     9241 ctggtcgctc cagccgcccg cgtgacggcc gctgagcgcg tccgcgaggc cgagcagggt
     9301 ctcgccctcg tcggcggcga gaagctgcag gcccacctcc agcgcgggcc aggcggtctc
     9361 caggtagagc gcctggacga cggcgtcgat cagctgggag ccggtgaaca ccctgtcccc
     9421 gccgacggag aggggttcgt cgtccagggt ctcctccagc tccatgacga gccgggtgat
     9481 ctcctccggg gtgtcgccga gggcgcagcc ggtgtccacg caccactgcg cgaagtggtc
     9541 gaaggcgagc tggaaaccgc ccgcctgcgc gagcgccccc tccatctggt ccagcgtcgg
     9601 gtcgacgacg ccgtccagga cggcgcggcc gacgtgccgg gggaacaagt gggcgtacac
     9661 gccgccgagt tcggtgccgt aggagatccc gaagtagtgc agctcgtcgt cgccgagcag
     9721 atggcgcagc aggtccatgt cgcgggcggc gtcctcggtg cccacgtgcg gcagcacgcg
     9781 gcccgactcc cgctcgcagg cgtccgcgta ccgctgccgc gcctggagca gctcggtcac
     9841 ctcgtcgtcg ccgtcgtcgg gggtgtcgtc ctgctgggcc atcgcctgcg ccgcctcggc
     9901 gtccaggcag gtgaccccgg cgctcgcccc gaccccgcgc gggtcgaagc tcaccaggtc
     9961 gtagcgggaa cggagttgct cgtagtcggg ggcgagggcg ggcagggtgg ccacgccgga
    10021 ctcgccgggc ccgccgaagt tgaacagcag cgagccgatg cggtcggcgg cgtccgcgcg
    10081 ggacgccgcc cggatcaggg cgacgtcgac ggtctcaccg ccgggtctgt cccagtccag
    10141 gggcgcccgc atggtggcgc actgccagcg ggtgccgtcc ggcaacggtc cgggcgcctc
    10201 accgccgcct tgcagggggg acggtgcggg gcagtccttc cagttcaggg actgcgccca
    10261 tatccgtccg cggtcgtcct cctcgccgcc tccgcctccg gccccgccct cgccggccgg
    10321 accgcaggcg gccagggagg cggagagcag gacggcgccg gacagcaggg cagcggctgt
    10381 cgtacgtcgt gtcggcatgc gcccagcctc cggcgggccg acggcagccg cacggccgca
    10441 caacccatcc gggggacgcc ccgggtcagc gcagcgcggg gtggtccgcg accacggtgc
    10501 acgagccggg ggcgatctcc gtgaagccag cgtcgcgcac cgtcggcagg ccggcaccgg
    10561 tcagcgcggg ccagtcggcg gggtcggcgg tgcgcacggc gagcgggaag cccgccgcgt
    10621 gccaggccgc gcgggccgcg tgcggcagtt cccaccaggc gagttgggcg ccgtgcccgg
    10681 cctgggccat cgccttgccg gcggacatct ccagctccgg gttcatccag agcgtgggca
    10741 cgccggggtc cgcgggcggc accgggtccg ggtcgtcgag gtcggtgccg gacacctgga
    10801 gccgggccag gtccttgggc cagccgtcca gcggcaccgg cgggaagacg cgtacctcgg
    10861 ccgacttccc ggtcacggtg atccccggga gcgcctccgc gcgccgccac tccgcgccgc
    10921 gcgcccgccg caccaccttg cggatccggg cgtcctgcca gtcgcggatc gcccgcgccc
    10981 actcgccgtc gcccaccgac cgttcgtcgg tcagcatcac cagcaccgcg cgggccgccg
    11041 tctccagcgc gtccgtccgc gcgggcggcg atccgcgctc gagacggacc accagcggca
    11101 gcacgaactg cggtgccgcg tcgcggtcac ccgggtcggg gcggaagggg ctgtcggggg
    11161 cgggtgtcgt ccggtcgtcg ttcacccgcc cagtgtgcca agcgggacgg gcagggcgac
    11221 gggcggcacc ggcggacatc aaggggcgcg gagggggcat ccgggtgggg gagaggatgg
    11281 tgcgcgatga ctcaggcggt gccgcagcgt g
//
