LOCUS       NZ_JMLS01000003        11932 bp    DNA     linear   CON 10-FEB-2024
DEFINITION  Paenibacillus sp. UNC451MF BP97DRAFT_scaffold00003.3_C, whole genome
            shotgun sequence.
ACCESSION   NZ_JMLS01000003
VERSION     NZ_JMLS01000003.1
KEYWORDS    WGS; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Paenibacillus sp. UNC451MF
  ORGANISM  Paenibacillus sp. UNC451MF
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 411338)
  AUTHORS   Dangl,J., Huntemann,M., Han,J., Chen,A., Kyrpides,N., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2014) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Paenibacillus sp. UNC451MF
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi0054671
            Gene Calling Method               :: Prodigal 2.5
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Unknown program v. before 2014-04-01
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/10/2024 01:27:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,772
            CDSs (total)                      :: 6,708
            Genes (coding)                    :: 6,634
            CDSs (with protein)               :: 6,634
            Genes (RNA)                       :: 64
            rRNAs                             :: 4, 3 (5S, 16S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 18 of 74
            Pseudo Genes (incomplete)         :: 59 of 74
            Pseudo Genes (internal stop)      :: 15 of 74
            Pseudo Genes (multiple problems)  :: 15 of 74
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:46:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 373002
            Orig. end                         :: 384934
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JMLS01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..11932
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11932
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11932
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6932](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6932)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(276..292)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.7"
                     /tool="antismash"
     misc_feature    complement(325..339)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: medium, score: 22.9"
                     /tool="antismash"
     gene            complement(624..908)
                     /locus_tag="BP97_RS40130"
     CDS             complement(624..908)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BP97_RS40130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_156043161.1"
                     /transl_table=11
                     /translation="MKLSKPVKGQNLYLTINRKVQLTAQQAILDQIATTQLIKQPAGEI
                     LQAVQLPRNDSVSSLKLMNSQEYALHLVTHWSQIPQLKKSVEEHLDFAV"
     gene            complement(859..1515)
                     /locus_tag="BP97_RS35575"
     CDS             complement(859..1515)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /locus_tag="BP97_RS35575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_028548474.1"
                     /transl_table=11
                     /translation="MIMKGVSIPPIRGNIYDSAGQQIAYSISTQSQYLTFKTSFKQEAA
                     EALAAKLVQLFNEKGEANSKPLTIEEVIEAMDIKGRTHLPFQQRRIKSGLSKEEIAYLS
                     EHRDEYPDMEIVEERISQYSPDRVAVQLVGYLNKMKGAKENLDFYKEINADQSDPTLKY
                     LDSEEVGYDGIELMYQKEMRGLNGYKSYQIGQHEPDRRRHEAEQAGQGAESVSND"
     gene            1947..2363
                     /locus_tag="BP97_RS0107130"
     CDS             1947..2363
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016742433.1"
                     /locus_tag="BP97_RS0107130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="VOC family protein"
                     /protein_id="WP_007729208.1"
                     /transl_table=11
                     /translation="MALTSAFTSFNLPVKNVEQSKAFFTGLGFELNPQFPENENSVAIV
                     IGDNLQVMLINQAFFNTLTEKESVDTSKYAQMTIALAFESREKVDEIVNTAVSLGGKLH
                     AEPEDHGFMYHWGFVDLDGHLWAINYMNMDAAQG"
     gene            complement(2443..2868)
                     /locus_tag="BP97_RS0107135"
     CDS             complement(2443..2868)
                     /NRPS_PKS="Domain: Polyketide_cyc2 (6-115). E-value:
                     5.6e-08. Score: 25.2. Matches aSDomain:
                     nrpspksdomains_BP97_RS0107135_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493207.1"
                     /locus_tag="BP97_RS0107135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SRPBCC family protein"
                     /protein_id="WP_028548475.1"
                     /transl_table=11
                     /translation="METSQPTKVTVQAVIQAPVEKVWRYWTEPDHITKWNQASEDWHAP
                     RAENDLRVGGKFLTRMEAKDGSMGFDFGGVYDVVNRHEAIGYTMEDGRRVDIAFVDQGN
                     ETKVIETFDAESSNPVEFQQAGWQAIMDNFKAYTEQN"
     aSDomain        complement(2524..2850)
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BP97_RS0107135_Polyketide_cyc2.1
                     "
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="5.60E-08"
                     /label="BP97_RS0107135_Polyketide_cyc2.1"
                     /locus_tag="BP97_RS0107135"
                     /protein_end="115"
                     /protein_start="6"
                     /score="25.2"
                     /tool="antismash"
                     /translation="TKVTVQAVIQAPVEKVWRYWTEPDHITKWNQASEDWHAPRAENDL
                     RVGGKFLTRMEAKDGSMGFDFGGVYDVVNRHEAIGYTMEDGRRVDIAFVDQGNETKVIE
                     TFDAE"
     gene            complement(2945..3901)
                     /locus_tag="BP97_RS0107140"
     CDS             complement(2945..3901)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493206.1"
                     /locus_tag="BP97_RS0107140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_028548476.1"
                     /transl_table=11
                     /translation="MNEKAFDEPLFASLKPGLTSFCYRMLGSMDDADDAVQETSIRVWQ
                     SWSTFRQDSSFKTWVYRIASNLCLDKLRQSKRRALPVDLFDPAVAIVEPRDTLPDSAWI
                     WPSPDFGGNPEDRLVRRDTLQLCFIALLQTLPPRQRAVLILKDVFEWSSKQIAETLAMS
                     PAAVNSALQRARETMDRAQLRSDELSSMDVQPEQQLLSRYVEAFEQFDIAALVALFHEE
                     GCMSMPPFEMWIRGKEDLSAFYSLTRWHCEGSRFVPITVNGGYPALAQYMPGKEGSGLV
                     PWGIHVIETKENQILHVQNFIHTPLFSRFGLPERIDQ"
     misc_feature    4019..4033
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 17.12"
                     /tool="antismash"
     misc_feature    complement(4883..4898)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.54"
                     /tool="antismash"
     gene            complement(5001..6932)
                     /locus_tag="BP97_RS0107145"
     CDS             complement(5001..6932)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677777.1"
                     /locus_tag="BP97_RS0107145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="WP_028548477.1"
                     /sec_met_domain="YcaO (E-value: 8.7e-74, bitscore: 243.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 2.8e-116, bitscore:
                     382.7, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTVVIIGEGLLADTVCRYLSEFPIVRRSAFGEDLPPAGLVLVLG
                     GNSSVHLQAEEMLQPLGIPWLRSYVSDGEGVVGPIVRPGTAGCSQCAENRYSMAREIKE
                     DMDDLIMRLISPDPLPRQVPEVSPLALRHIANIVSVEAAKVLQGDKPYSENHIYLVNLI
                     DLNTTIHYILPDQSCPVCGQWPDDSKEAAQITFRSCRKLSRDNYRSRSMNDLQKVLVKD
                     YWNSRTGLFNVREVSFLSTFACAAVNLPLPMSNELTGGHSHSFADSELAAILEGLERYC
                     GVAPRGKRTVVHNSFVNLKDDAIDPLKVGLHTQEHMDRPDFPYLRFDPDADIPWVWGYS
                     LLYERPILVPELLAYYSPSYGGGFVYETSNGCALGGSREEAILYGILEIVERDSFLMTW
                     YAKLPVPRLDYRSSGDKELMTMIERIRAVTGYEVLLYNTTMENGIPSIWAIAKGGATQA
                     LNLVCAAGAHLDPIRAAKSALYELAGTITRVEANWEERRNDAEAMFYDSFLVENMEDHS
                     ILYSLPQAEERLCFLLDEQRPLRTFGEEFGPVATNDDLTDDLKQVLEVFRSLGLDVIVV
                     DQSTSETLRNELYCFKVLIPGMLPMTFGHTLTRLAGLNRVLEVPMQLGYVDHKLSPEEL
                     NPYPHPFP"
     gene            complement(6929..8467)
                     /locus_tag="BP97_RS0107150"
     CDS             complement(6929..8467)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677776.1"
                     /locus_tag="BP97_RS0107150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_028548478.1"
                     /transl_table=11
                     /translation="MFSQGEGVLAVGSGTVLVALVKAWYESRLSKLSVLAINIPLAEAE
                     QLKVVWEQALPSDPETSLDIILAEAEGGKVNWDTAIRPFSFILYASQHNDLEELEKLQS
                     TCIAEKKPLLPAMDLRGIGMIGPLLYPDGDGCGKSAWRRIHASVFQPGWELYPFYRTTA
                     TLLSNLIVYEYHKWLVGEIEPSSNNHCYIFNPLTLEGSWHPVLPHPFLSGHEAVRITTE
                     LERNLESDQEPDVEQWFSWFEGLTSKVSGIFHVWEEGALEQLPLAQCLVQSVDPLSEGF
                     AQLLPAIFCSALTHVEARRESGLTGVESYAARMTPLWIPWLSSSQQEDISIGAGLTFAE
                     AVGRGLIACLTKVLDRRTLHQRRVLTRMEIDRIEDVHCRYYLQALNIKDSETLIASGKP
                     LFGFPVVWVLSDNSWYGCIGLDMTLALRRALQNALMKTEIAAVSSVIWSDDKPQRITIS
                     SAASINQASRMLSALETLNQHHKRLEIFDMRCEPFLKKGPLEVVGVLLAEEVSL"
     misc_feature    8774..8787
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 21.22"
                     /tool="antismash"
     misc_feature    complement(8779..8792)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 21.22"
                     /tool="antismash"
     misc_feature    complement(8779..8795)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.36"
                     /tool="antismash"
     gene            complement(8920..9585)
                     /locus_tag="BP97_RS0107160"
     CDS             complement(8920..9585)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /locus_tag="BP97_RS0107160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_028548479.1"
                     /transl_table=11
                     /translation="MRLVDRIDLFSKLKEEDKRTLEGAMRTKTYAKGNNIFLEYDPADA
                     LYFVLDGIVSIYLDRTCYVSHKGEFFGVEGVFSDGLLHLVAAKAEENTKLILLSSAVFL
                     KLFDDKKEEKEKNGRNVHLLKTILLHTNRRMYQSFHKIHARAGGNIGAVANMLNIYIEK
                     GEYTVTEKGKKIKHKLSERWIAEYCVCHRSTVQRALSQLYEEGNIEKPDRYITILDSE"
     misc_feature    9729..9739
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            complement(9773..10240)
                     /locus_tag="BP97_RS0107165"
     CDS             complement(9773..10240)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /locus_tag="BP97_RS0107165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2269 family protein"
                     /protein_id="WP_028548480.1"
                     /transl_table=11
                     /translation="MTKILLIIHLIAVCCWLGGAIYERIFIVGGIHRAKGTELEASLTK
                     LLLSTVTFFLTSVLTILITGIIMTVSSGYGFLEWTWIGVKQYILLAIILVFFLYIGPRM
                     GRIGKQLKASLEKNEGVDEETRSLTRHNAVIFDIVHLGVLINLILGVTKFF"
     gene            complement(10532..10648)
                     /locus_tag="BP97_RS40935"
     CDS             complement(10532..10648)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362355.1"
                     /locus_tag="BP97_RS40935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YjcZ family sporulation protein"
                     /protein_id="WP_231506236.1"
                     /transl_table=11
                     /translation="MGFGLGGVGGSCCGGIFTSTGVILVLFILLVIVSRSFI"
     misc_feature    10785..10801
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.78"
                     /tool="antismash"
     misc_feature    complement(10823..10841)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.47"
                     /tool="antismash"
     misc_feature    10826..10844
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 23.91"
                     /tool="antismash"
     misc_feature    complement(11385..11398)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.31"
                     /tool="antismash"
ORIGIN
        1 tggtaatgcg gctattccga tcattgaatt ataagttccc atcgcagttc cgcgttgtcc
       61 ttcaggtaca atatcggcaa tgtatgcctt ttgtatacct tctgtaaacg cataataaag
      121 tccataaaat atgaacaata cccaaatcca catgacactc tttgccatcc caaaaccaaa
      181 ataaatcaat gcaaacataa taaatccata aattaatacc ggtcgacgac cgatccggtc
      241 agataacatt cctataggca tggaaaagat gctgctgctc atatttaatg caaaaaaggc
      301 tagaggaatc agtgcaggaa gcattcctac atcttgtgcg cgtaaagcca aaaaagcatc
      361 agaaaagttc ccaacagcaa ataaagtaga tattaaagtg aatataataa agcggcggtt
      421 taaattacga aatcctatct tcggaagcga tgttttcttt tgattttcgt catgtctttt
      481 ctcttttaag aaaaatacag taagagcaac cgcaacgagt cctggtataa ctgacagcca
      541 aaatacaagg tgaaaattgt ttgcatatat agcaagtatc ccaaatgcaa ctaacggtcc
      601 aagtgcggct ccgagagcat ccattacacg gcgaaatcca aatgctcttc cacgctcttc
      661 tttagttgtg gaatctgcga ccaatgcgtc acgaggtgaa gtgcgtattc ctgcgaattc
      721 atgagcttga gtgaggaaac tgaatcgttt cgagggagct gaacagcctg caggatctcg
      781 ccggcaggct gtttaattaa ctgggttgtc gcgatctgat cgagaatggc ttgctgcgcg
      841 gtgagctgca ccttgcgatt aatcgttaga tacagattct gccccttgac cggcttgctc
      901 agcttcatgt cgccgacgat ccggctcatg ctgtccgatt tgataggatt tataaccgtt
      961 aaggccccgc atctccttct gatacatcaa ctcgatgccg tcgtagccga cctcctcgga
     1021 atccaaatac ttaagcgtgg gatccgattg atccgcgttg atttctttgt aaaagtccaa
     1081 attctccttg gctcccttca tcttgttcag atagccgacc aattgaacgg cgacccggtc
     1141 gggactgtac tggctgatgc gctcctccac gatctccatg tccggatact cgtcgcggtg
     1201 ctcgctaaga taggcgatct cttcttttga cagtcccgat ttgatccgtc gttgttggaa
     1261 tggcagatgc gttctgcctt tgatgtccat agcctcgata acttcctcaa tcgtcaacgg
     1321 cttggaattc gcttcgcctt tttcgttaaa cagctgcacg agttttgcag ccagcgcctc
     1381 cgccgcttcc tgcttaaagc ttgttttgaa agtaaggtat tgggactgag ttgagatcga
     1441 atatgcgatc tgctgtcccg ccgaatcgta aatattgccg cggattggcg ggatggatac
     1501 gcccttcatg atcatggttg tctggcgttc ctagagactc ggaccctcga cgaactgtag
     1561 ataagcaaaa cgtacgatta gtgaagagaa caggagaaat gtaagcatga aaagacattt
     1621 aacctaatac tgaaatggcg acggtttcga acttttcttt cgtacttcgg ttttatcatt
     1681 aatttccata ccgttccctc attccgtcac tcgataccca ccctttaatt gtcatatatt
     1741 cagacgaatt cctttttaga gaggttcaat ctttatccct gcacaataag aataagcaac
     1801 cttacgggaa tggcgggtat ccgaactcaa taccaagtta tttttcaaaa tttacgcttc
     1861 cgaatccata agaccgatga attcccggat cggctgtcgt ctatatatat gaacacaaac
     1921 taatttgacg aagaggtgtt ttgaaaatgg cattaacgtc cgcattcacg agcttcaacc
     1981 tgcctgtaaa aaacgtagaa caatcgaaag cgttcttcac cggactcgga ttcgagctca
     2041 acccgcaatt ccccgagaac gagaattcgg tagccatcgt gatcggcgac aacctacagg
     2101 tcatgctgat caatcaagca ttctttaata cgctcacgga gaaggaatcc gtcgatacga
     2161 gcaagtatgc gcagatgacg atcgcattgg ccttcgagag ccgggaaaag gtcgatgaaa
     2221 tcgtgaatac cgcggtctcc ttgggcggga aattgcacgc tgaacctgaa gatcatgggt
     2281 tcatgtatca ttggggcttc gtggacttgg acggccatct gtgggccatt aactacatga
     2341 acatggatgc ggcacaaggt taaggctaac ggagagcacg ctcgtactag ggttcaaaaa
     2401 gaaaaacgcc gggcatcttc cccggcgtct tcttcgtgat gattagtttt gttcggtata
     2461 ggctttgaaa ttgtccatga tcgcttgcca gcctgcttgc tggaactcga cgggattgga
     2521 gctttccgcg tcgaacgttt caatgacctt cgtctcattc ccttgatcga cgaaagcaat
     2581 atccactctt cttccgtctt ccatggtgta gccgatcgcc tcatgccgat tcacgacatc
     2641 gtagacgcca ccaaaatcaa agcccatgct gccatccttc gcttccatcc gtgtcagaaa
     2701 cttgccgccg acccgcagat cgttctcggc tctcggcgca tgccagtcct cggacgcttg
     2761 attccacttc gtgatgtgat ccggctcggt ccaatagcgc cataccttct cgacaggggc
     2821 ttgaatgacg gcttgcacgg ttactttcgt cggttgactc gtttccattt taaatagaca
     2881 cctctctcat gatattcgtc gtttggttat tctcaatgat atagacgtaa gccaatgacg
     2941 aaattcattg gtcaattcgc tcaggcagcc caaatcgcga aaataatggc gtatgaatga
     3001 agttttgaac gtgcaggatt tgattttctt tggtttcgat gacgtggatg ccccagggta
     3061 ccaggccaga gccctctttg cccggcatat actgggccaa cgccggataa ccgccattca
     3121 ccgtaatggg cacaaatcgc gaaccttcgc aatgccagcg agtaagcgaa tagaaggcgg
     3181 acaaatcttc cttgccgcgg atccacatct cgaagggcgg catcgacatg cagccttcct
     3241 cgtggaacaa cgcgacgagc gcagcaatat cgaattgctc gaaggcctcc acataccggg
     3301 acagcagctg ctgctccggt tgaacgtcca tgctgctgag ctcatccgag cgaagctgcg
     3361 cccggtccat cgtctctcgg gctctttgca aggcgctgtt caccgctgcc ggcgacattg
     3421 ccaacgtttc cgcgatctgc ttggaggacc attcaaatac atccttcaaa atgagtaccg
     3481 cgcgctgccg cggaggcaag gtctgcagga gagcgatgaa gcacagttga agggtatctc
     3541 tgcggacgag ccgatcctcc ggattgcccc caaagtcggg agacggccag atccaggcag
     3601 agtccggcag cgtgtcgcgc ggctcgacga tggcgacggc cggatcgaac aggtcaacgg
     3661 gaagcgcgcg gcgtttggat tgtctcagct tgtccaagca caggttggag gcaatccgat
     3721 agacccaagt tttgaacgag gaatcctgtc tgaacgtgct ccagctctgc cagacccgaa
     3781 tactcgtctc ctgaacggca tcatccgcat cgtccatgga gcctagcatt cggtaacaga
     3841 acgaggttaa gcctggcttc aagcttgcaa ataatggttc gtcaaatgct ttctcgttca
     3901 tcgcggcctc ccttaacgat ctccccgaag ggtagtacct tcattatatg caatgaagcg
     3961 gacagcctca atagaataaa taggcaggat tatgggttca tttatcatgg gccttcgagg
     4021 acttggacgg ccatatgtgg gcgataaact tcatgaacaa tactgcgtcg gaaggccagg
     4081 gataacgaag agcatcaacg ttaaaagtta aaattagcta gtgattggat tggactcttc
     4141 agttctgtcc tgaaccgtca gttctgctgt gattgatcgc tatctcccgc ttccttcagt
     4201 gtggatggtg gggggaggtt cacactttga atcgatgctc ctcctttcca tcttcttctg
     4261 cctttatgag ttcaagctat taacagattg gacgtatttt aaaatgtacg aattatcagg
     4321 gtgattccat cgttccggag tcaattagat tttcataaac taggtatgta gggacctact
     4381 ccaccctgct ccactcatag tatgcctttc ttaagaagta gacggtcgct aacgcaccgt
     4441 ctatctttac ttacataact atgggacata tgtatcaaat atcttatttt gtgatgatgt
     4501 atccacgatt accataaaag cgattaaacg attaaaaggc ccttgtcatc ctttttcatt
     4561 tttctttccg aagtgacttc gataaaggaa aagaaaatcg attatagggc cttttaatgc
     4621 ttctaatgac tttttattta cttggtagta cgctcataat cataaagtcc tggtgccatc
     4681 aaccacttgc cattgacata ttcaattttt acaatcaatg tatcgtacgg ttcgtcaaat
     4741 aatattgtat ctaacgcaat atctacaaac tttatctttt tgacttctta tcttggcggt
     4801 ttcagtcaga attttggttg cccatccgtg tcctccattt aaaatagagt tgcccgttat
     4861 atataaagag gaaggccttt atagttgttt ataccagtta tgggaaaagc agattctggg
     4921 aatggatgat caaccagagg ttcacttctt aatgaggaca gcgcatcttg ctttataccg
     4981 ataagactta cgcaacatgc ctacggaaac ggatgcggat agggattcag ctcctccggc
     5041 gacagcttgt gatcgacata tcccagctgc atcggtactt ccaacactct gttcagaccc
     5101 gccaagcggg taagcgtatg tccgaatgtc atcggcagca ttcccggtat cagcactttg
     5161 aagcagtaca gttcgttgcg aagcgtctcg ctcgttgact ggtcgactac aataacatcc
     5221 aaccccaaac tgcgaaacac ctcaagaacc tgcttcagat catctgttaa atcgtcatta
     5281 gtcgctactg ggccgaattc ttcgccgaaa gtccgtagag gacgctgttc gtctagcaaa
     5341 aaacatagtc gttcctccgc ttgtggcaaa ctatacaata tagaatgatc ctccatgttt
     5401 tctaccagga atgaatcata aaacatagct tcagcatcat ttcggcgttc ttcccagttt
     5461 gcctcaaccc tggtgatcgt cccagccagc tcatacaagg cgctcttggc tgcacgaata
     5521 ggatccagat gggctcctgc ggcgcacaca aggttcaatg cctgagtcgc tccccccttc
     5581 gctatagccc agatgcttgg aatgccgttt tccatcgtcg tattgtacaa cagcacttca
     5641 tatcccgtta cggctcttat gcgttcgatc atcgtcatca gttctttgtc gccagacgaa
     5701 cggtaatcga gccgtggaac aggtaacttg gcgtaccagg tcatcaagaa cgaatcgcgc
     5761 tcaacaattt ccaaaatgcc gtacaagatt gcttcttccc gactcccgcc taacgcgcag
     5821 ccgttagaag tctcatatac aaagcctcct ccataactcg ggctgtaata agccagcagc
     5881 tcggggacta gaatcggacg ttcatacagt aatgagtatc cccacaccca aggaatatcc
     5941 gcatcaggat cgaaccgtag atagggaaaa tccggccgat ccatgtgctc ctgcgtgtgg
     6001 agccccacct tcaacggatc tatggcatca tccttcaagt taacgaagct gttatgcaca
     6061 accgttcgtt ttcctcgagg agcgaccccg caatatctct ccaatccttc gaggatggca
     6121 gccagctcac tatctgcaaa cgaatgcgaa tggcctcctg tcaattcatt cgacataggc
     6181 aaaggaagat tcacagccgc gcatgcaaaa gtggacagga agctcacctc cctgacgttg
     6241 aagagcccgg tccggctgtt ccaataatcc ttaaccagaa ccttctgtaa atcgttcatc
     6301 gaacggctgc ggtaattgtc cctgctcagc ttcctgcaag atctaaatgt tatctgggct
     6361 gcttccttcg aatcgtccgg ccattggccg caaaccggac aagattggtc cggcaggatg
     6421 tagtgaattg tcgtattcag atcaatcaag ttaactaaat agatgtggtt ttcggaatac
     6481 ggtttatctc cttgaagcac cttagccgct tcaacagaga ctatatttgc aatatgtcgc
     6541 aatgccaaag gagatacctc tggaacctgt ctagggagag gatcggggga gatgagtctc
     6601 atgatcagat cgtccatatc ttccttgatt tccctcgcca tggagtacct attttccgca
     6661 cactgggaac agccggccgt tccggggcgg actatcggcc caaccacccc ttctccatcg
     6721 gatacatagc tgcgtagcca tgggatgcca agcggctgca gcatctcttc tgcttggaga
     6781 tgaacggagg agttccctcc cagcaccaat accagccccg caggaggtaa atcctcaccg
     6841 aaagcggatc tgcgtactat cgggaattct gacaaatacc tgcacactgt atccgccaac
     6901 agcccctctc cgataataac gacggtattc atagggatac ctcctcagcg agcagtacgc
     6961 caacgacttc aagcggccct tttttcagaa aaggctcgca acgcatgtcg aagatctcca
     7021 gccgtttatg atgctgattc aaggtttcta gcgctgaaag cattcttgag gcctgattga
     7081 tggaagctgc agaagaaatg gtgatgcgtt gcggcttatc gtcgctccaa ataacggaac
     7141 taaccgcggc tatttccgtt ttcatcaaag cattttgcag cgctcggcga agcgctaagg
     7201 tcatatcaag gcctatacag ccataccagg aattatccga gaggacccac acgaccggaa
     7261 atccgaatag tggctttccg gatgcaatga gagtctcgct atctttaata ttcagagcct
     7321 gcaaataata acggcaatgg acatcttcga tacggtcaat ttccatgcga gtgagaactc
     7381 tcctttgatg aagggtccgt ctgtctagca ctttggtcaa acaagcgatc agcccgcgcc
     7441 caaccgcttc agcgaacgtg agtcctgctc cgatactgat gtcctcctgt tggctcgagg
     7501 atagccaagg tatccataac ggggtcatgc gagcggcata ggattctacc cctgtaagac
     7561 cggactcccg gcgggcctct acatgcgtta gagcgctgca gaatatggca ggcaaaagtt
     7621 gcgcaaagcc ttcggatagc gggtctacgg actgcacgag acattgtgcc agcggaagct
     7681 gctccaatgc tccttcctcc caaacatgga aaatgcccga gaccttggag gttagccctt
     7741 cgaaccaaga aaaccattgc tccacatcag gttcctgatc cgattcaaga tttcgctcca
     7801 gctccgtcgt tatgcggacc gcttcgtgac ccgataagaa aggatgcggg agaaccggat
     7861 gccagcttcc ctccagcgtg agcggattga agatataaca atgattattg ctactcggct
     7921 cgatctctcc gacaagccac ttgtggtatt catatacgat aagattggat aagagagttg
     7981 cggtcgtcct atagaaggga tacaattccc atccaggttg gaatacggac gcatgaatgc
     8041 gtcgccacgc actcttccca cacccgtctc cgtccgggta aagcaggggc ccgatcatgc
     8101 ctattcctcg tagatccatt gcagggagca gcggtttctt ttcggctatg catgtgctct
     8161 gaagcttttc aagttcctct aggtcgttat gctgagaagc atacaaaata aatgagaacg
     8221 gcctgattgc agtgtcccaa ttcaccttgc cgccctccgc ttcggctagg ataatatcca
     8281 gagatgtttc cggatcgctc ggaagcgcct gttcccagac caccttaagc tgctccgctt
     8341 ctgcaagtgg tatgttgatt gccaatacgg aaagcttcga taaacgcgat tcataccatg
     8401 ctttaaccag cgcaaccaag acggttccgg aaccgactgc cagtacccct tcaccttgag
     8461 aaaacatttg tctacctcca atccatgaca tcacacagtt tgaaactcgg tcatgccatt
     8521 atcaacaaac cctaaatcta ctgtaaaaat atgtcggaat ccttaatttt attaacgtaa
     8581 ccaaagcaag gctgcattaa attcttcagc agcaagtccg tgaaaaggtt gagttcactg
     8641 ctgaattgag aagcattttt tacgcaagcg aatccatgat atagtgtgca acttctagtt
     8701 tgatctcctg attatcaatc tgcgctgcga ggtttcctac cgtgctggat cagttctcgt
     8761 agtctaagag cagtattatt ataataaaga tacaaatgca aaataacccg atccgcgtaa
     8821 tgttatttaa gatgtaatct aacagatccg ctccgatctc tcgaatttta ttcatcattt
     8881 atgttcctcc taaatttctt cgtattgttt ctttcgtaat cattccgaat ccagaatggt
     8941 aatatatcga tcgggctttt cgatattccc ttcctcatac aattgtgaca aagctctttg
     9001 gacagtagat ctatgacata cgcaatactc cgctatccac ctttcactta atttgtgctt
     9061 tattttcttt cctttttccg taaccgtgta ttctcccttt tcgatataga tattaagcat
     9121 attggccaca gcaccgatat tcccgcctgc ccgcgcgtgg atcttatgga atgattgata
     9181 catacgacga tttgtatgta gcagaatagt tttaagaaga tgtacgttcc ggccgttctt
     9241 ttccttttcc tctttcttgt cgtcgaacag cttcaggaat acagcacttg aaaggaggat
     9301 caattttgtg ttttcttctg cctttgccgc aaccagatgt aagagcccat cggaaaaaac
     9361 accctctact ccaaaaaatt cgcccttgtg agagacatag catgtccgat cgaggtatat
     9421 cgatacaatc ccatctaaga cgaaatacaa tgcatcggcc ggatcgtatt caagaaagat
     9481 gttatttcct ttagcataag ttttggttct cattgcgcct tcgagagttc tcttgtcttc
     9541 ctccttcaat ttcgagaaca aatcaatgcg gtctactagc ctcacgctgt acaccccctt
     9601 atccgcatat gatgtgtttg ttttgtttag atgtaaaaga caaaaaaact gccaacaacg
     9661 gcagcccctt tttccaggtt tagctttatg aagctgtttc cttaacccac ttctgattaa
     9721 ctaaaccatg caatgtctca tagaaagctc tagctgctgg tcttgctaag catcaaaaga
     9781 acttcgtaac gccaagaatg agattgatga gaacgcccaa atggactata tcaaaaataa
     9841 cggcattatg acgcgtcaac gagcgggttt cctcatcgac accttcgttc ttttccaagc
     9901 ttgcttttaa ctgtttgcct atgcgcccca ttcgcggtcc aatatagagg aagaaaacca
     9961 agatgatggc caatagaata tactgtttga ccccgatcca cgtccattca agaaacccat
    10021 agccgctcga caccgtcatg ataataccag taattagaat ggtcaacaca gacgttaaaa
    10081 agaacgttac cgtagataac aagagcttgg tcagggaagc ctccaattca gttcccttcg
    10141 ccctgtgaat gccgccaact ataaaaattc gctcatatat cgccccgccg agccaacaac
    10201 aaacggcaat taggtgaata attaataata tttttgtcac ggaatcaact ccttcattgt
    10261 ttgtgtaaat cattcctata tgtatgggtt aattatggag atatgccgag agacataacc
    10321 ggaagaagcg gctgcgacgc cattaagaag cagggtgcta agatcattaa agagatcgag
    10381 tccaagccat ggggtgtccg ctagtttacg tttcgcgata ttaacggtcg tatggaagag
    10441 aagagcttgg catgaggctg gtatgtggga gacacaaata aaaccaatcc ccatctatgg
    10501 acaaggattg gttatttgca ttattggacg attagataaa agaacggctt acgatgacca
    10561 gaagtatgaa cagtaccaag atgacgcctg tgcttgtaaa gatacctccg cagcagctgc
    10621 caccaactcc acctaaacca aaacccatag tcgattctcc tctcaacact cttttttaag
    10681 ggaatgctta ttcccaaaat caatatatgt gaagggagtt caaaattctt ggacgggtat
    10741 cattgaggga taaatggggc tggtgcttgg atatccctcc gtcatgggaa ttttattaca
    10801 attgaagtga ttatttctat aaggtatagt atatacagtt taagtattcg attcggtgaa
    10861 gggggtgtca aaaaacgcgt aaaccgcggc ttcttatgca tatatgttct tatcctccta
    10921 catttaccac tatttgtggt acggttcccc gtatcagctc taaagcgaaa tatcagcgcg
    10981 ccgcggcgtt ctgatatttc gcatgttaaa tagagtgagc gctaaaattt ttaggttgct
    11041 gcgcaccaag ccaactcggg ctcgcaaacg ttgcagtccg attattggcc taacgtgttt
    11101 ttgttaaagt gtcagcactt ccccttaacc gctcttccaa aatcaagcgc agctcatcag
    11161 gaatccgttc aaactcgacc cctttcccca aaaacaaaag ccaatccgca taatattcta
    11221 gcgcctcaac atcggtcaca tccagctgag cattgaaaat cccactcgac tggaacatcc
    11281 ctgtaaagga caaaataatc cccggtgggt gcaagcgctt aaagcgctga atccctgtcg
    11341 tatcaagctt tacaatcaga ttggactcac gtgcgccaag ccgttttttg cttaaaatct
    11401 cttgctcaga ccgcctcatc ttctgctcag aaatggccga atcccgcagc atctcaacag
    11461 gcaaataacg aaactcatcg gtatcaagat cataaacctc gaccagccaa cgatcgtttg
    11521 agttaaaaat atgaatcaaa aaaacatcca tcaactgcgg ccagccagga ctcggctcat
    11581 acgtcaagcg caagtgctta tcccgcgcgg caagcgaaat cagctggttc aactccgcag
    11641 gcgccgcatc atcgagttcc aaaagattcg gattagcagg atttgtattt tcaaaaagca
    11701 agatattatt cagctcgatc agctcgtcgt gctgggtttg cgacgcgatg ccgatgagtt
    11761 tttcagtgat ggagcggcga ttttgcaaat aaggcagctg cgaatttttc gaggcgataa
    11821 agctgataaa tatcgctttc agctcttctg gcgtgaaacg gacggcggac agatactgat
    11881 tttgcaggac attgtagccg cccccgcgcc caagctcggt cgtcagcgga aa
//
